##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_7765_flu.3510000003b9bd.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	311929
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.77539760650661	34.0	34.0	34.0	34.0	34.0
2	33.87125595888808	34.0	34.0	34.0	34.0	34.0
3	33.892286385683924	34.0	34.0	34.0	34.0	34.0
4	33.896912438407455	34.0	34.0	34.0	34.0	34.0
5	33.894280429200236	34.0	34.0	34.0	34.0	34.0
6	37.66610671018084	38.0	38.0	38.0	38.0	38.0
7	37.676615511863275	38.0	38.0	38.0	38.0	38.0
8	37.67121364156587	38.0	38.0	38.0	38.0	38.0
9	37.59196483815227	38.0	38.0	38.0	38.0	38.0
10-14	37.66485578448942	38.0	38.0	38.0	38.0	38.0
15-19	37.6310769437917	38.0	38.0	38.0	37.6	38.0
20-24	37.62528844705046	38.0	38.0	38.0	37.4	38.0
25-29	37.53384071375217	38.0	38.0	38.0	37.0	38.0
30-34	37.47016724959847	38.0	38.0	38.0	37.0	38.0
35-39	37.39239698777606	38.0	38.0	38.0	37.0	38.0
40-44	37.38868396333781	38.0	38.0	38.0	37.0	38.0
45-49	37.35533855460698	38.0	38.0	38.0	37.0	38.0
50-54	37.304126900672905	38.0	38.0	38.0	37.0	38.0
55-59	37.253372402053024	38.0	38.0	38.0	37.0	38.0
60-64	37.07342824809492	38.0	38.0	38.0	36.4	38.0
65-69	37.04157163970007	38.0	38.0	38.0	36.4	38.0
70-74	37.12635567709319	38.0	38.0	38.0	37.0	38.0
75-79	37.02592833625601	38.0	38.0	38.0	36.2	38.0
80-84	36.91287376293964	38.0	38.0	38.0	36.0	38.0
85-89	36.95768524247505	38.0	38.0	38.0	36.0	38.0
90-94	36.810258744778466	38.0	38.0	38.0	35.8	38.0
95-99	36.80110217389214	38.0	38.0	38.0	36.0	38.0
100-104	36.811879626453454	38.0	38.0	38.0	36.0	38.0
105-109	36.63767203434115	38.0	38.0	38.0	35.0	38.0
110-114	36.53705683024022	38.0	37.8	38.0	34.2	38.0
115-119	36.61228228218601	38.0	38.0	38.0	34.8	38.0
120-124	36.52239900746645	38.0	37.6	38.0	34.2	38.0
125-129	36.48803926534564	38.0	37.0	38.0	34.0	38.0
130-134	36.351860198955535	38.0	37.0	38.0	34.0	38.0
135-139	36.13034953466975	38.0	37.0	38.0	33.6	38.0
140-144	36.082202039566695	38.0	37.0	38.0	33.2	38.0
145-149	35.644570399033114	38.0	37.0	38.0	30.2	38.0
150-154	35.31632134235675	38.0	36.4	38.0	28.0	38.0
155-159	35.34484898807101	38.0	36.0	38.0	28.6	38.0
160-164	35.3230164556678	38.0	36.0	38.0	28.6	38.0
165-169	35.072964681065244	38.0	35.6	38.0	28.0	38.0
170-174	34.90608055038166	38.0	35.0	38.0	27.2	38.0
175-179	34.630284455757554	38.0	35.0	38.0	25.0	38.0
180-184	34.27098602566609	37.6	34.8	38.0	23.4	38.0
185-189	33.49990029782418	37.0	33.8	37.8	22.2	38.0
190-194	32.48699992626528	36.8	30.6	37.0	20.2	38.0
195-199	32.22506980755236	36.8	30.0	37.2	20.0	37.8
200-204	32.0391473700746	37.0	29.8	37.0	19.6	37.4
205-209	30.915473713569433	35.4	27.4	37.0	16.4	37.2
210-214	30.09142721580872	34.4	25.6	37.0	15.0	37.0
215-219	29.260517617791226	33.2	23.8	37.0	11.8	37.0
220-224	27.75674336147008	31.4	21.2	36.2	9.0	37.0
225-229	26.59546050543553	29.4	19.8	35.8	9.0	37.0
230-234	26.18777670559647	29.0	19.2	35.8	9.0	37.0
235-239	25.2529094761949	27.4	17.6	34.6	8.8	37.0
240-244	24.154248562974267	25.8	15.8	33.6	8.0	37.0
245-249	23.164204674781764	24.0	14.0	32.4	8.0	36.2
250-254	22.35884255712037	23.0	13.0	31.4	8.0	36.0
255-259	21.61682498260822	21.6	12.2	30.8	8.0	36.0
260-264	20.473502623994563	20.2	10.8	29.2	7.4	35.2
265-269	19.646675365227345	18.8	9.0	28.2	7.0	34.2
270-274	18.53765119626582	18.0	8.2	26.2	7.0	33.0
275-279	17.479954733288668	16.0	8.0	24.6	7.0	31.4
280-284	17.19082227045257	15.6	8.0	24.0	7.0	30.8
285-289	16.015899772063516	13.4	8.0	21.6	7.0	29.4
290-294	15.2718426308551	12.8	8.0	20.6	7.0	28.0
295-299	14.6239080047062	12.6	8.0	19.2	7.0	27.0
300-301	12.96353336817032	10.5	8.0	16.5	7.0	22.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.0382269619815645
1101	2	0.04024249085095022
1101	3	0.03294877207537894
1101	4	0.04014360095550984
1101	5	0.009033081114438346
1101	6	0.0534885758519863
1101	7	0.05249935355983126
1101	8	-0.0121104969714807
1101	9	-0.0010533532856911165
1101	10-14	-0.008055900687779172
1101	15-19	0.06102062602634817
1101	20-24	-0.018325092426643153
1101	25-29	0.05262223273717126
1101	30-34	0.03958082277707575
1101	35-39	0.09771754341764449
1101	40-44	0.040864474034329135
1101	45-49	0.08468539414363363
1101	50-54	0.004407244128806553
1101	55-59	0.12631573802051577
1101	60-64	0.29119324813478187
1101	65-69	0.22122899527163753
1101	70-74	0.2345853468338035
1101	75-79	0.10702087785285386
1101	80-84	0.1824709398157225
1101	85-89	0.11843454527686248
1101	90-94	0.32151143858906295
1101	95-99	0.16099988233885654
1101	100-104	0.22004523590430125
1101	105-109	0.14957572588316026
1101	110-114	0.3361049041965529
1101	115-119	0.1972002007010758
1101	120-124	0.11831624155171028
1101	125-129	0.2453680768417783
1101	130-134	0.27868432916435637
1101	135-139	0.31708220329238657
1101	140-144	0.1992811992108301
1101	145-149	0.5600357713997042
1101	150-154	0.8912484494072643
1101	155-159	0.5860531502829218
1101	160-164	0.40585107978323265
1101	165-169	0.3734001998351104
1101	170-174	0.35712275802509197
1101	175-179	0.5500033047944584
1101	180-184	0.1928901272628636
1101	185-189	0.3099999042213142
1101	190-194	0.668005372194159
1101	195-199	0.23795130507853912
1101	200-204	0.16604123087235223
1101	205-209	0.1147157534431571
1101	210-214	-0.2340744048832306
1101	215-219	-0.08050193460485389
1101	220-224	0.3478250345316276
1101	225-229	0.5920597370673129
1101	230-234	0.42556856526624287
1101	235-239	0.5717102658885445
1101	240-244	0.5150818316157633
1101	245-249	0.5131982860103577
1101	250-254	0.02185268004035379
1101	255-259	-0.13670776855173372
1101	260-264	0.2075494922575416
1101	265-269	0.019558849652117516
1101	270-274	0.06599222007250205
1101	275-279	0.617310460536391
1101	280-284	-0.09074498993029323
1101	285-289	-0.1622047521467298
1101	290-294	-0.1638019298200195
1101	295-299	-0.013595040200367947
1101	300-301	-0.15953568227934412
1102	1	-0.06586149317416812
1102	2	-0.02510407054339936
1102	3	0.04676501366154184
1102	4	0.025586131812481483
1102	5	-0.016674311054956092
1102	6	0.05478947522809108
1102	7	0.07444659136183418
1102	8	0.05778246016635791
1102	9	-0.007974978224545737
1102	10-14	0.061132440132851684
1102	15-19	0.0032477897656093546
1102	20-24	0.018550828481075143
1102	25-29	0.0435111356303679
1102	30-34	0.0846936724219205
1102	35-39	0.0420876650449884
1102	40-44	0.01952882398976641
1102	45-49	0.08116291274184562
1102	50-54	0.11381843336390318
1102	55-59	0.003990384136876912
1102	60-64	0.18269073786412804
1102	65-69	0.30222806198512586
1102	70-74	0.17795616167395423
1102	75-79	-0.055100893530955375
1102	80-84	0.02881296681801615
1102	85-89	0.14606162679088186
1102	90-94	0.29154483884294535
1102	95-99	0.0895824571893371
1102	100-104	0.043504938032846496
1102	105-109	0.1757140503487662
1102	110-114	0.3145555885566509
1102	115-119	0.09957542989643287
1102	120-124	0.21597132166130706
1102	125-129	0.08156759009235515
1102	130-134	0.20895252149939836
1102	135-139	0.1681965954107696
1102	140-144	0.2861236598462469
1102	145-149	0.26007088217870944
1102	150-154	0.7439833390183779
1102	155-159	0.49399748919496034
1102	160-164	0.488637495212771
1102	165-169	0.5189616872117355
1102	170-174	0.4187346728829482
1102	175-179	0.3898108257033783
1102	180-184	0.5094375073085402
1102	185-189	0.546345262246227
1102	190-194	0.47041481189214096
1102	195-199	0.4388023663543521
1102	200-204	0.035689913017776576
1102	205-209	-0.1996019420893731
1102	210-214	-0.6871000837666479
1102	215-219	-0.4754477829803001
1102	220-224	0.5757888194135852
1102	225-229	0.7287420985522921
1102	230-234	0.3377772133211927
1102	235-239	0.7297603872758103
1102	240-244	0.8401047136405424
1102	245-249	0.8247872438204098
1102	250-254	0.7421007962120179
1102	255-259	0.34283014958913327
1102	260-264	0.407770960248218
1102	265-269	0.542778177985916
1102	270-274	0.5169577664952527
1102	275-279	0.7110793475847146
1102	280-284	0.3537841805250359
1102	285-289	0.6139220187719392
1102	290-294	0.6348599593799644
1102	295-299	0.458866105371321
1102	300-301	0.27284005707263503
1103	1	-0.11262638264336289
1103	2	-0.03150131805340095
1103	3	0.02779189024276718
1103	4	0.05679287915211262
1103	5	0.058173334040283464
1103	6	0.0461155735252774
1103	7	0.1080973495932227
1103	8	0.0104144834937685
1103	9	0.14575044220262612
1103	10-14	0.08001298966591008
1103	15-19	0.04840605492496053
1103	20-24	0.07118405798288308
1103	25-29	0.041446466589540876
1103	30-34	0.06363627962821283
1103	35-39	-0.5122170206410459
1103	40-44	-0.2936576777124458
1103	45-49	-0.32548431441396275
1103	50-54	0.0061414348082990955
1103	55-59	-0.3378888958976276
1103	60-64	0.06055170817112554
1103	65-69	0.20559479629806532
1103	70-74	0.0658880851904371
1103	75-79	0.2912394225470507
1103	80-84	0.19046265155418496
1103	85-89	0.1742752523400668
1103	90-94	0.28399599631806893
1103	95-99	0.23668236160001044
1103	100-104	0.1932620446616795
1103	105-109	0.04250203805784736
1103	110-114	0.22292764511416863
1103	115-119	-0.040892626082118966
1103	120-124	0.2569676820300373
1103	125-129	0.32929952989218236
1103	130-134	0.31693226983111344
1103	135-139	0.4433789539038955
1103	140-144	0.38597138032739053
1103	145-149	0.4933932594278616
1103	150-154	0.7753152269527135
1103	155-159	-0.03238968675874787
1103	160-164	-0.6674517255081014
1103	165-169	0.36525948564190713
1103	170-174	0.7147865625059282
1103	175-179	0.3594559390385399
1103	180-184	0.4761109084489803
1103	185-189	0.5238428725131925
1103	190-194	0.018108757198163516
1103	195-199	0.2413341264342037
1103	200-204	0.22944584965743076
1103	205-209	0.6016802213050418
1103	210-214	0.7584908964611401
1103	215-219	0.5436574165360248
1103	220-224	1.0864860786837909
1103	225-229	1.8416647496030158
1103	230-234	0.968760215308361
1103	235-239	1.4490160570493806
1103	240-244	1.4494383927438719
1103	245-249	1.3277743347388196
1103	250-254	1.5689050069668689
1103	255-259	0.8385729570203715
1103	260-264	1.651443520082001
1103	265-269	0.6163352164658953
1103	270-274	0.6509773125515181
1103	275-279	1.5456184532667052
1103	280-284	0.4943381764300767
1103	285-289	0.797483972412623
1103	290-294	1.2725115981186672
1103	295-299	1.2381105577229121
1103	300-301	-0.801279360224509
2116	1	0.02595835839451155
2116	2	0.004712107574185609
2116	3	0.030870617914388276
2116	4	0.026049046382347285
2116	5	0.0370103395938699
2116	6	-0.30687373202324153
2116	7	-0.1031647694576705
2116	8	0.0518078756695175
2116	9	0.06313019297918032
2116	10-14	0.1142998210816728
2116	15-19	0.10848439884171768
2116	20-24	-0.18673714917956374
2116	25-29	-0.19044081085073827
2116	30-34	-0.027357935086257612
2116	35-39	-0.06801193625118174
2116	40-44	-0.1705728678883247
2116	45-49	0.025296590523439022
2116	50-54	0.12867769240193638
2116	55-59	0.09146212394902165
2116	60-64	0.2985472044820128
2116	65-69	0.2910846629975623
2116	70-74	-0.7961779640894804
2116	75-79	0.16672298987430878
2116	80-84	-0.13346815672044698
2116	85-89	0.028158779119635824
2116	90-94	0.14142194249101436
2116	95-99	0.09873711545385788
2116	100-104	0.059026911542183313
2116	105-109	0.18783912687899829
2116	110-114	0.31550321947934634
2116	115-119	0.2148083057905339
2116	120-124	-0.30331817913759096
2116	125-129	-0.31536403294585824
2116	130-134	0.04535373403691523
2116	135-139	0.2983427868184805
2116	140-144	0.3566551156379134
2116	145-149	0.5927463790652467
2116	150-154	0.8999631499700342
2116	155-159	-0.6110100518327073
2116	160-164	-0.7670573992875092
2116	165-169	0.47751082335341977
2116	170-174	0.6495922594554386
2116	175-179	0.32435380956109583
2116	180-184	0.5641193655372234
2116	185-189	0.8486248925118858
2116	190-194	0.7478430974656334
2116	195-199	0.8714871215322688
2116	200-204	0.665878812027735
2116	205-209	0.6228359750937145
2116	210-214	0.840354931390074
2116	215-219	1.1949713786963478
2116	220-224	1.30606134170068
2116	225-229	0.9376605210588984
2116	230-234	1.227221865164644
2116	235-239	1.1679595002717633
2116	240-244	1.7173418602948658
2116	245-249	1.1555283551140292
2116	250-254	0.8479457646292978
2116	255-259	1.0187926385762474
2116	260-264	0.4395251078134308
2116	265-269	0.8313743522209869
2116	270-274	0.90721057758131
2116	275-279	1.5211299695328364
2116	280-284	-0.2481866409243345
2116	285-289	-0.7606030458121094
2116	290-294	0.39256778562274164
2116	295-299	0.7644163017366985
2116	300-301	1.2970176370747275
1117	1	-0.0258706711976302
1117	2	0.01068418279420058
1117	3	0.04707597958812926
1117	4	0.022844801119845215
1117	5	0.0544915998336819
1117	6	-0.3089470671930954
1117	7	-0.16211998251823445
1117	8	-0.05292744787226411
1117	9	0.16360575517198583
1117	10-14	0.12764774992133
1117	15-19	0.12210983561949718
1117	20-24	0.09623917420272221
1117	25-29	0.1673281065203298
1117	30-34	0.09214417449602763
1117	35-39	-0.11380770548751684
1117	40-44	-0.054307770963532676
1117	45-49	0.1544639217206054
1117	50-54	0.17649286109602969
1117	55-59	0.26466738413083846
1117	60-64	0.2657989479684133
1117	65-69	0.2327763885053855
1117	70-74	-0.05081513043474928
1117	75-79	0.24489729501704716
1117	80-84	0.0741312691252034
1117	85-89	0.21513591443738278
1117	90-94	0.3722601208558274
1117	95-99	0.32393785199529646
1117	100-104	0.30299060642844466
1117	105-109	0.3176902237168022
1117	110-114	0.35874777153745185
1117	115-119	0.320349765244508
1117	120-124	0.46012123742878686
1117	125-129	0.3680849528597676
1117	130-134	0.2555472936489309
1117	135-139	0.3624929368815515
1117	140-144	0.47275695554163377
1117	145-149	0.9369223370765809
1117	150-154	0.8507569618619897
1117	155-159	0.08421866748410878
1117	160-164	-0.44557358725055707
1117	165-169	0.42319988107332307
1117	170-174	0.7516566053399458
1117	175-179	0.8413128213196615
1117	180-184	0.6119330843465676
1117	185-189	1.063341512540596
1117	190-194	1.2386099415015224
1117	195-199	1.007075992217061
1117	200-204	0.8743275803506521
1117	205-209	1.3718580278405206
1117	210-214	0.9017178230401903
1117	215-219	0.5632931371165526
1117	220-224	1.3257800640622541
1117	225-229	0.6846495454314585
1117	230-234	1.3455133209938985
1117	235-239	1.0968493235308152
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2108	180-184	-0.3077495131927819
2108	185-189	-0.7242560123450659
2108	190-194	-0.40284677726519647
2108	195-199	-1.1797265800967232
2108	200-204	-0.6992555786890406
2108	205-209	-0.24690840710961837
2108	210-214	-0.10370075651726296
2108	215-219	-0.697192107950432
2108	220-224	-0.502723382757214
2108	225-229	-0.02196059513166304
2108	230-234	0.01585620937943233
2108	235-239	-0.6079765130063386
2108	240-244	-0.12394960694985357
2108	245-249	-0.04747266792949034
2108	250-254	-0.36658030867213043
2108	255-259	-0.619546167682202
2108	260-264	0.008683851258442843
2108	265-269	-0.634564308092795
2108	270-274	-0.5535705125681396
2108	275-279	-0.15564780109078669
2108	280-284	-0.3648138882218497
2108	285-289	0.28475598522975254
2108	290-294	-0.10149240939940185
2108	295-299	0.09902049836136761
2108	300-301	-0.20901831968558682
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	44.0
14	179.0
15	334.0
16	409.0
17	509.0
18	953.0
19	871.0
20	860.0
21	1149.0
22	1704.0
23	2593.0
24	3687.0
25	5531.0
26	7581.0
27	10566.0
28	14489.0
29	20288.0
30	27325.0
31	37148.0
32	48480.0
33	55918.0
34	48320.0
35	21064.0
36	1919.0
37	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.004263790798547	22.544553407987074	14.800483443347684	32.65069935786669
2	44.36842999528739	23.472008053114653	17.255208717368376	14.904353234229584
3	43.747134764641956	22.187100269612635	19.166541103905054	14.899223861840355
4	44.10779376075966	22.065277675368435	18.57794562224096	15.248982941630947
5	28.27374178098221	22.213708888881765	34.66237509176768	14.850174238368346
6	27.052951152345567	22.023280938931617	35.02720170295163	15.896566205771185
7	17.608494240676563	47.17451727797031	19.971531983239778	15.245456498113352
8	26.53456395525905	20.669767799723655	36.30858304293606	16.487085202081243
9	16.131876164127092	19.42781850998144	37.852844717868486	26.58746060802298
10-14	26.426718900775498	34.0317187565119	23.32524388562782	16.216318457084785
15-19	21.841059984804232	35.47288004642082	21.359988971849365	21.326070996925584
20-24	26.6920356876084	30.394544912464056	25.670905879222516	17.24251352070503
25-29	19.05581077745256	27.241840290578946	35.996396615896565	17.705952316071926
30-34	19.277912601906202	28.858361999044657	30.26791353160495	21.595811867444194
35-39	21.289331867187723	29.983425715467305	28.10261309464654	20.624629322698436
40-44	19.836244786473845	30.88837523923713	27.927829730483538	21.347550243805482
45-49	21.574332620564295	28.999740325522794	26.67716050767963	22.748766546233277
50-54	23.193867835308676	31.073737933952916	24.62406509173562	21.108329139002784
55-59	21.920565256837293	27.750802265900255	28.33773070153785	21.990901775724602
60-64	20.794411548781934	27.325192591903928	29.27980405797473	22.600591801339405
65-69	19.06440247620452	28.604714534397253	28.613434467458944	23.71744852193929
70-74	20.523324218011147	26.48237259119864	30.722952979684482	22.271350211105734
75-79	21.71866033616624	25.49535310920113	28.26547066800458	24.520515886628047
80-84	22.611299366201923	28.071580391691697	25.5117029836918	23.805417258414575
85-89	21.674868319393195	26.707616156240682	26.791737863424046	24.825777660942073
90-94	22.357587784399655	29.640463054092436	27.53895918622507	20.462989975282834
95-99	24.074645191694263	27.81408589775237	27.71188315289697	20.39938575765639
100-104	22.90854649615778	26.85694500992213	27.80062129523064	22.433887198689444
105-109	24.265009024489547	28.24822315334579	24.927852171487743	22.558915650676916
110-114	21.916012938841853	27.11136188042792	29.496520041419682	21.476105139310548
115-119	25.030247267807738	27.028009579102935	27.414315437166792	20.527427715922535
120-124	20.69265762401059	27.750096977196737	26.915355738004482	24.64188966078819
125-129	19.983329539735003	25.193040724010913	30.06831682850905	24.75531290774503
130-134	23.68833933363041	28.976081095377477	26.93010268362352	20.4054768873686
135-139	23.060375918878975	26.518983486626762	25.519974096669433	24.900666497824826
140-144	21.600941239833425	28.03734183099359	26.820013528719677	23.541703400453308
145-149	22.565840303402375	25.818952389806654	29.001663840168757	22.613543466622215
150-154	21.7339202190242	28.177309580064698	25.44630348572912	24.64246671518198
155-159	21.311388168461413	26.96164832381728	30.497132360248646	21.229831147472662
160-164	22.574047299225146	30.882091758060326	24.840332255096513	21.703528687618014
165-169	21.2114295240263	29.13002638420923	26.553093813015145	23.10545027874933
170-174	21.42025909742281	30.8091905529784	26.063687569927772	21.706862779671017
175-179	19.23597998262425	28.805465346280723	28.03997063434307	23.91858403675195
180-184	20.933738126304384	29.309554417832263	27.071801595875982	22.68490585998737
185-189	20.57455382474858	27.962388876955973	28.034584793334382	23.428472504961064
190-194	19.851184083557477	26.87701367939499	27.850504441715902	25.421297795331633
195-199	19.336451564298287	32.11121761682947	26.38125983797595	22.171070980896292
200-204	20.9033465948982	27.92257212378458	25.537542197102546	25.636539084214675
205-209	20.58051672015106	27.50029654184125	28.565154249845314	23.35403248816237
210-214	22.59379538292368	26.921190399097227	28.15589445033966	22.32911976763943
215-219	21.14994117251041	28.52078517867848	28.414478935911696	21.91479471289941
220-224	19.786938694382375	28.227833898098602	26.959468340551858	25.02575906696716
225-229	22.17876503948014	28.102356626027074	27.028843102116184	22.690035232376598
230-234	21.374671800313532	28.111910082102014	27.307496257161084	23.205921860423366
235-239	21.06767886281814	28.296182785185092	25.71283849850447	24.9232998534923
240-244	21.53611879626453	30.823873381442574	25.327494397763594	22.3125134245293
245-249	22.013406897082348	29.12124233399267	24.78237034709822	24.08298042182676
250-254	19.483985137643504	28.754299856698157	24.768841627421626	26.992873378236716
255-259	20.44452423468161	30.294073330790024	24.25359617092351	25.00780626360486
260-264	18.486771028022403	29.495365932632104	26.34509776263188	25.672765276713612
265-269	19.17231164784294	29.81973461909601	25.16053332649417	25.84742040656688
270-274	17.966460316289925	31.989138553965805	24.633362079191098	25.411039050553168
275-279	18.893209672713983	30.455905029670216	24.482173828018556	26.16871146959725
280-284	16.398026473973243	31.537369080784412	26.74140589685473	25.323198548387616
285-289	18.064623680388806	30.716092444113883	25.436621795344454	25.782662080152857
290-294	19.101141605942377	31.513581616329354	24.642017895097922	24.743258882630343
295-299	17.73448444998702	31.54192139877985	23.948142045144888	26.775452106088242
300-301	14.471562438888336	34.378015509939765	26.441754373591426	24.708667677580475
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.75
31	4.0
32	9.0
33	34.0
34	125.5
35	268.25
36	525.75
37	1046.75
38	1990.75
39	3943.25
40	6033.75
41	6839.25
42	7862.75
43	10380.25
44	12077.25
45	11117.75
46	10658.75
47	10464.25
48	7884.75
49	5704.25
50	4593.75
51	1734.25
52	377.5
53	95.75
54	49.25
55	34.25
56	23.75
57	19.5
58	17.5
59	15.25
60	12.5
61	10.0
62	3.25
63	2.0
64	1.5
65	1.25
66	1.25
67	0.5
68	1.25
69	0.25
70	0.5
71	0.25
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	311929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.060469107374706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	35.51787289985795	3.928443359685401
2	8.357358811765575	1.8487261791355785
3	7.012942871684968	2.326993139521657
4	6.292559144536394	2.7839462409789197
5	5.697828837385355	3.1510329917504727
6	4.781743621042208	3.173299655995404
7	3.986800382278229	3.0867117725820616
8	3.481008522755582	3.080126978275694
9	3.1819153612622815	3.1674128899970246
>10	20.796635516291207	42.89994441058752
>50	0.7517127251331731	5.3632613245589145
>100	0.08102337904742904	1.5069581046244391
>500	0.008656846708527384	0.7497129911457351
>1k	0.03462738683410136	8.07258011283347
>5k	0.011542462278033787	7.516917993810695
>10k+	0.005771231139016893	7.343931854516988
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	11840	3.7957355680299045	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	11170	3.5809430992309146	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	6663	2.136063014339802	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	6261	2.007187533060408	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	5411	1.7346896248825856	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	5217	1.6724959846631766	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	4377	1.4032039342286224	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	2852	0.9143106283801763	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	2700	0.8655815906824951	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA	2677	0.8582081178729776	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	2616	0.8386523856390395	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	2285	0.7325384943368523	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	1614	0.5174254397635359	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	1551	0.49722853598094435	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	1294	0.4148379919789439	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	1212	0.38854995848414225	No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	1105	0.3542472806311693	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT	1010	0.3237916320701185	TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	948	0.30391531406185385	No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	832	0.26672736423993926	No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	569	0.18241330559197766	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	446	0.1429812553497751	No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	429	0.13753129718621865	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	3.2058577432685E-4	0.0	0.0
6	3.2058577432685E-4	0.0	0.0
7	3.2058577432685E-4	0.0	0.0
8	6.411715486537E-4	0.0	0.0
9	9.6175732298055E-4	0.0	0.0
10-14	0.0012823430973074	0.0	0.0
15-19	0.00134646025217277	0.0	0.0
20-24	0.0019235146459611	0.0	0.0
25-29	0.0019235146459611	0.0	0.0
30-34	0.0051293723892296	0.0	0.0
35-39	0.007052887035190701	0.0	0.0
40-44	0.01102815063684364	0.0	0.0
45-49	0.01487517992876584	0.0	0.0
50-54	0.02173571549936043	0.0	0.0
55-59	0.02898095399914724	0.0	0.0
60-64	0.039047347313010336	0.0	0.0
65-69	0.054435464480699136	0.0	0.0
70-74	0.07732528876763621	0.0	0.0
75-79	0.11278207540818583	0.0	0.0
80-84	0.16285757335803983	0.0	0.0
85-89	0.23614348136915772	0.0	0.0
90-94	0.3381538747599614	0.0	0.0
95-99	0.45600120540251143	0.0	0.0
100-104	0.5850049209916359	0.0	0.0
105-109	0.7520301094159247	0.0	0.0
110-114	0.9616932058256846	0.0	0.0
115-119	1.2105318838581858	0.0	0.0
120-124	1.5049578589999648	0.0	0.0
125-129	1.8450352484058872	0.0	0.0
130-134	2.2483962696639295	0.0	0.0
135-139	2.7000375085355963	0.0	0.0
140-144	3.204575400171193	0.0	0.0
145-149	3.776051601486236	0.0	0.0
150-154	4.433829493250066	0.0	0.0
155-159	5.159443334861459	0.0	0.0
160-164	5.998288071965095	0.0	0.0
165-169	6.912598700345271	0.0	0.0
170-174	7.905196374816063	0.0	0.0
175-179	8.930942618352253	0.0	0.0
180-184	10.057416912181939	0.0	0.0
185-189	11.260126503146548	0.0	0.0
190-194	12.583568696722653	0.0	0.0
195-199	14.004468965694116	0.0	0.0
200-204	15.436269150992695	0.0	0.0
205-209	16.946484616691617	0.0	0.0
210-214	18.577560919311768	0.0	0.0
215-219	20.079377037723326	0.0	0.0
220-224	21.635820972080182	0.0	0.0
225-229	23.196176052883832	0.0	0.0
230-234	24.78884617973962	0.0	0.0
235-239	26.334197846304768	0.0	0.0
240-244	27.78177085170023	0.0	0.0
245-249	29.18176892818558	0.0	0.0
250-254	30.579458787095785	0.0	0.0
255-259	31.874432963911666	0.0	0.0
260-264	33.16370071394452	0.0	0.0
265-269	34.34704692413978	0.0	0.0
270-274	35.4438349752668	0.0	0.0
275-279	36.380586607849864	0.0	0.0
280-284	37.24180823200152	0.0	0.0
285-289	38.028397487889876	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCCGC	10	8.4620767E-4	294.99997	295
GGACCGG	20	6.177652E-8	294.99997	295
CGGGTTA	4880	0.0	292.58197	1
GGGTTAT	4920	0.0	290.80286	2
GGGGGGA	3285	0.0	290.50992	1
ATTAGTA	5045	0.0	283.89	7
GGTTATT	5110	0.0	279.99023	3
TATTAGT	5125	0.0	279.17075	6
TTATTAG	5125	0.0	278.88293	5
GTTATTA	5360	0.0	266.6558	4
TTAGTAG	5455	0.0	262.8231	8
GGAGCGA	2325	0.0	262.64514	5
GCGAAAG	2330	0.0	262.08154	8
CGAAAGC	2330	0.0	261.4485	9
GGGGGAG	3665	0.0	259.98636	2
GAGCGAA	2380	0.0	257.19537	6
TAGTAGA	5660	0.0	252.78268	9
GGGGAGC	4300	0.0	220.90698	3
AGCGAAA	2885	0.0	211.66379	7
GGGAGCG	2895	0.0	210.42314	4
>>END_MODULE