FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_7765_flu.3510000003b9bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_7765_flu.3510000003b9bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311929
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA118403.7957355680299045No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC111703.5809430992309146No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC66632.136063014339802No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG62612.007187533060408No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA54111.7346896248825856No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG52171.6724959846631766No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG43771.4032039342286224No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG28520.9143106283801763No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG27000.8655815906824951No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA26770.8582081178729776No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG26160.8386523856390395No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC22850.7325384943368523No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA16140.5174254397635359No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA15510.49722853598094435No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA12940.4148379919789439No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT12120.38854995848414225No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC11050.3542472806311693No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT10100.3237916320701185TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA9480.30391531406185385No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA8320.26672736423993926No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA5690.18241330559197766No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT4460.1429812553497751No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA4290.13753129718621865No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCGC108.4620767E-4294.99997295
GGACCGG206.177652E-8294.99997295
CGGGTTA48800.0292.581971
GGGTTAT49200.0290.802862
GGGGGGA32850.0290.509921
ATTAGTA50450.0283.897
GGTTATT51100.0279.990233
TATTAGT51250.0279.170756
TTATTAG51250.0278.882935
GTTATTA53600.0266.65584
TTAGTAG54550.0262.82318
GGAGCGA23250.0262.645145
GCGAAAG23300.0262.081548
CGAAAGC23300.0261.44859
GGGGGAG36650.0259.986362
GAGCGAA23800.0257.195376
TAGTAGA56600.0252.782689
GGGGAGC43000.0220.906983
AGCGAAA28850.0211.663797
GGGAGCG28950.0210.423144
Produced by FastQC (version 0.11.2)