##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_7637_flu.3510000003b9ca.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329597
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.817756229577334	34.0	34.0	34.0	34.0	34.0
2	33.883758043914234	34.0	34.0	34.0	34.0	34.0
3	33.89170714539271	34.0	34.0	34.0	34.0	34.0
4	33.899016071141425	34.0	34.0	34.0	34.0	34.0
5	33.88455295406208	34.0	34.0	34.0	34.0	34.0
6	37.65841011902414	38.0	38.0	38.0	38.0	38.0
7	37.66258491430444	38.0	38.0	38.0	38.0	38.0
8	37.66936288861852	38.0	38.0	38.0	38.0	38.0
9	37.58106111402713	38.0	38.0	38.0	37.0	38.0
10-14	37.66692597323398	38.0	38.0	38.0	38.0	38.0
15-19	37.63382251658844	38.0	38.0	38.0	37.6	38.0
20-24	37.63136436314651	38.0	38.0	38.0	37.2	38.0
25-29	37.545963707193934	38.0	38.0	38.0	37.0	38.0
30-34	37.48475198499987	38.0	38.0	38.0	37.0	38.0
35-39	37.407176642991296	38.0	38.0	38.0	37.0	38.0
40-44	37.41384357260533	38.0	38.0	38.0	37.0	38.0
45-49	37.38399499995449	38.0	38.0	38.0	37.0	38.0
50-54	37.34778350531103	38.0	38.0	38.0	37.0	38.0
55-59	37.30530799734221	38.0	38.0	38.0	37.0	38.0
60-64	37.13032703574365	38.0	38.0	38.0	36.6	38.0
65-69	37.06398419888531	38.0	38.0	38.0	36.4	38.0
70-74	37.150707682412154	38.0	38.0	38.0	37.0	38.0
75-79	37.060815480723434	38.0	38.0	38.0	36.8	38.0
80-84	36.95589219562071	38.0	38.0	38.0	36.2	38.0
85-89	37.00241264331897	38.0	38.0	38.0	36.0	38.0
90-94	36.84724678926083	38.0	38.0	38.0	35.8	38.0
95-99	36.859824573645994	38.0	38.0	38.0	36.0	38.0
100-104	36.84767458441673	38.0	38.0	38.0	36.0	38.0
105-109	36.708195766344964	38.0	38.0	38.0	35.2	38.0
110-114	36.603328913794726	38.0	38.0	38.0	34.8	38.0
115-119	36.703426912259516	38.0	38.0	38.0	35.0	38.0
120-124	36.61476287708929	38.0	37.8	38.0	34.8	38.0
125-129	36.531191728080046	38.0	37.4	38.0	34.2	38.0
130-134	36.4161918949505	38.0	37.0	38.0	34.0	38.0
135-139	36.18247617545063	38.0	37.0	38.0	33.6	38.0
140-144	36.1461730537596	38.0	37.0	38.0	33.8	38.0
145-149	35.724961088844864	38.0	37.0	38.0	30.6	38.0
150-154	35.35096375270406	38.0	36.4	38.0	28.6	38.0
155-159	35.45584213448545	38.0	36.2	38.0	29.4	38.0
160-164	35.413467962390435	38.0	36.0	38.0	28.6	38.0
165-169	35.194594004193	38.0	36.0	38.0	28.2	38.0
170-174	35.048258327594	38.0	35.4	38.0	28.0	38.0
175-179	34.68989523569693	38.0	35.2	38.0	25.4	38.0
180-184	34.39216497722977	37.6	35.0	38.0	23.6	38.0
185-189	33.63028243582314	37.0	33.8	37.8	22.4	38.0
190-194	32.623029335825265	36.8	31.0	37.0	20.4	38.0
195-199	32.369576786196475	36.8	30.4	37.2	20.2	37.8
200-204	32.16340318631541	37.0	30.0	37.0	19.8	37.4
205-209	31.000850129097046	35.4	27.6	37.0	16.6	37.2
210-214	30.28631267881686	34.4	26.0	37.0	15.2	37.0
215-219	29.40806500059163	33.2	24.2	37.0	12.6	37.0
220-224	27.847823857620064	31.4	21.4	36.2	9.0	37.0
225-229	26.743552277478255	29.8	20.0	36.2	9.0	37.0
230-234	26.304442091402528	29.0	19.2	35.8	9.0	37.0
235-239	25.257601252438583	27.4	17.6	34.6	8.6	37.0
240-244	24.092861282111187	25.8	15.6	33.2	8.0	37.0
245-249	23.153251394885267	24.0	14.0	32.2	8.0	36.2
250-254	22.20788417370304	22.6	13.0	31.2	8.0	36.0
255-259	21.526801518217702	21.4	12.2	30.4	7.8	36.0
260-264	20.231914125431967	19.8	10.2	29.0	7.4	35.0
265-269	19.362585824506898	18.6	8.8	27.6	7.0	33.8
270-274	18.373239440892966	17.6	8.0	26.0	7.0	32.8
275-279	17.117519880338715	15.4	8.0	24.0	7.0	31.0
280-284	16.765994229316412	14.6	8.0	23.4	7.0	30.0
285-289	15.673887201643216	13.2	8.0	21.2	7.0	28.6
290-294	15.000123180732833	12.8	8.0	20.4	7.0	27.2
295-299	14.24906658737792	12.0	8.0	19.0	7.0	26.0
300-301	12.643352336338012	10.0	8.0	16.0	7.0	22.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.035604151723688915
1101	2	0.07483101556265837
1101	3	0.05679981020450242
1101	4	0.02929928325752229
1101	5	0.06937351753461485
1101	6	0.10826754134978245
1101	7	0.06427094386122434
1101	8	0.0820767819856627
1101	9	0.07258835536973152
1101	10-14	-0.009903532873195786
1101	15-19	-0.04125716303347815
1101	20-24	-0.0071712682619491375
1101	25-29	0.056103520165777354
1101	30-34	0.10307796200840613
1101	35-39	0.17009875544877673
1101	40-44	0.15700011577793305
1101	45-49	0.20528690341902234
1101	50-54	0.18283660824179293
1101	55-59	0.14163011573643303
1101	60-64	0.347088724634979
1101	65-69	0.27329804739314056
1101	70-74	0.24210900957596948
1101	75-79	0.07804183528692477
1101	80-84	0.24544732523116153
1101	85-89	0.13277360546465644
1101	90-94	0.29869185415513755
1101	95-99	0.15097799241955556
1101	100-104	0.1304153296926387
1101	105-109	0.1291829009089085
1101	110-114	0.3307754224710422
1101	115-119	0.18536195986757775
1101	120-124	0.3000231367882904
1101	125-129	0.10918535291130382
1101	130-134	0.10554674638331818
1101	135-139	0.2363539082052597
1101	140-144	0.27382773355936507
1101	145-149	0.4600987632361324
1101	150-154	0.5899661732138313
1101	155-159	0.24175527451303225
1101	160-164	0.02123139549325259
1101	165-169	0.18053847655883004
1101	170-174	0.3252065997471263
1101	175-179	0.35484291644084465
1101	180-184	0.12948550015752858
1101	185-189	0.26741266402322594
1101	190-194	0.4770789809473328
1101	195-199	0.08243584525153125
1101	200-204	-0.039443961521577364
1101	205-209	-0.29566208919487735
1101	210-214	-0.16637060909256718
1101	215-219	-0.16440144902282938
1101	220-224	0.369573318125088
1101	225-229	0.8393894314986277
1101	230-234	0.3750779955390655
1101	235-239	0.7730348727568561
1101	240-244	0.3201453690989986
1101	245-249	0.5823233810956481
1101	250-254	0.5923370501170027
1101	255-259	-0.12999894239012733
1101	260-264	0.49305849589955386
1101	265-269	0.46737927360847564
1101	270-274	0.1658699087941926
1101	275-279	0.5083213415776804
1101	280-284	0.21853425917159086
1101	285-289	0.5383119675079584
1101	290-294	0.40887260906482226
1101	295-299	0.10890297172649888
1101	300-301	0.11903050613318022
1102	1	-0.052926504944949215
1102	2	0.011515014502158749
1102	3	0.0616369752145971
1102	4	0.03844877546340797
1102	5	0.05955039328873113
1102	6	0.05443920118995038
1102	7	0.10988313741275135
1102	8	0.017267427969031246
1102	9	0.14433693100318834
1102	10-14	0.11435479554541672
1102	15-19	0.015962062262616428
1102	20-24	0.08573638733623312
1102	25-29	0.06709427328098627
1102	30-34	0.07081829173640841
1102	35-39	0.08846807894907016
1102	40-44	0.009713851919784133
1102	45-49	0.0020020679854297896
1102	50-54	0.041080619950541575
1102	55-59	0.0402758177475846
1102	60-64	0.21961860522084464
1102	65-69	0.20238650691299398
1102	70-74	0.10936886922243616
1102	75-79	-0.06873149429498682
1102	80-84	0.1352530270258967
1102	85-89	0.1439732115657648
1102	90-94	0.23402426045535663
1102	95-99	-0.06816235570482831
1102	100-104	0.07069582033483357
1102	105-109	0.17404192514342043
1102	110-114	0.19167121781676144
1102	115-119	0.045151267243973336
1102	120-124	0.14155403999630067
1102	125-129	-0.008386974609138065
1102	130-134	0.10767531541572595
1102	135-139	0.14199077454475884
1102	140-144	0.1582503308031562
1102	145-149	0.48401567654049416
1102	150-154	0.7199268374312666
1102	155-159	0.26246688966082843
1102	160-164	0.3087229412758816
1102	165-169	0.2880907303855409
1102	170-174	0.4688612023583403
1102	175-179	0.5285133724004609
1102	180-184	0.5358425305494308
1102	185-189	0.8829419115572961
1102	190-194	0.6238588033681864
1102	195-199	0.5331113710362132
1102	200-204	0.2728204701656409
1102	205-209	0.5764457568599148
1102	210-214	0.38702433406803394
1102	215-219	0.38742547353383117
1102	220-224	1.0485484234984526
1102	225-229	1.3050996669181494
1102	230-234	0.7089170488831478
1102	235-239	1.5716984300750987
1102	240-244	1.4335682314670457
1102	245-249	1.7370985936008587
1102	250-254	1.2919934707231064
1102	255-259	1.3267354842651393
1102	260-264	1.399867319667674
1102	265-269	1.3928333665318249
1102	270-274	1.377233459078525
1102	275-279	1.4405534172841818
1102	280-284	0.8380885257783817
1102	285-289	1.3044555999267047
1102	290-294	0.928162292484819
1102	295-299	0.7474147048275341
1102	300-301	0.4541759727789234
1103	1	0.02521641509397199
1103	2	0.04949258778577814
1103	3	0.014220722095998894
1103	4	0.030151542657129937
1103	5	0.05662611471043277
1103	6	0.17585927000289558
1103	7	0.11110611637207768
1103	8	0.07183403140315647
1103	9	0.17853957589901626
1103	10-14	0.11072010207829663
1103	15-19	0.06466434570021562
1103	20-24	0.07233906049646066
1103	25-29	0.10510608997546456
1103	30-34	0.23616178576011748
1103	35-39	-0.29930539273488677
1103	40-44	-0.09982864524019419
1103	45-49	-0.07144406930346747
1103	50-54	0.11317495782974163
1103	55-59	-0.23278385819016734
1103	60-64	0.2570645903168085
1103	65-69	0.3746319506369318
1103	70-74	0.23799418651754678
1103	75-79	0.3472786591481025
1103	80-84	0.31158213338157736
1103	85-89	0.3006645371118921
1103	90-94	0.4400788216233238
1103	95-99	0.3931447642069088
1103	100-104	0.33584581190986285
1103	105-109	0.292565979780683
1103	110-114	0.45493945243304523
1103	115-119	0.14942788877818458
1103	120-124	0.43370849408566414
1103	125-129	0.33939182382762567
1103	130-134	0.5473204884536571
1103	135-139	0.6092176561658249
1103	140-144	0.5455139561476301
1103	145-149	0.8032131849247008
1103	150-154	1.0118867019795417
1103	155-159	0.1426247354785133
1103	160-164	-0.4302982407196936
1103	165-169	0.5331648429104874
1103	170-174	0.9089031641252916
1103	175-179	0.6244628660871641
1103	180-184	0.7306744932404143
1103	185-189	0.8754822834516105
1103	190-194	0.4464393433269933
1103	195-199	0.8155999429772649
1103	200-204	0.5872184655672399
1103	205-209	1.04828843284659
1103	210-214	1.0302933296490053
1103	215-219	0.7906758798346196
1103	220-224	1.35593195513556
1103	225-229	2.045838454918819
1103	230-234	0.9814462237256549
1103	235-239	1.3545343280406037
1103	240-244	1.251838993573184
1103	245-249	1.5851355764845891
1103	250-254	1.7166074170316143
1103	255-259	0.16791237050635388
1103	260-264	1.5718447951129733
1103	265-269	0.7338466453655919
1103	270-274	0.7450674818096275
1103	275-279	1.2820232749294291
1103	280-284	0.6839632818620203
1103	285-289	0.516432096988833
1103	290-294	0.5784685807708527
1103	295-299	0.8324253311558252
1103	300-301	-0.7568667119044861
2116	1	0.049251658481942684
2116	2	0.023975897509359356
2116	3	0.03436684309207294
2116	4	0.03865883573454454
2116	5	-0.008697650188359773
2116	6	-0.19453877479854498
2116	7	-0.06150449919375234
2116	8	0.08769148040869368
2116	9	0.12065936813322509
2116	10-14	0.12294787648325212
2116	15-19	0.11273898364083124
2116	20-24	-0.16600576637402753
2116	25-29	-0.15345231431683715
2116	30-34	-0.008163831595901172
2116	35-39	-0.16475473036468458
2116	40-44	-0.1413351028957095
2116	45-49	0.02553959067463296
2116	50-54	0.18950813030544822
2116	55-59	0.1756865933008811
2116	60-64	0.285502662723502
2116	65-69	0.23501479179781626
2116	70-74	-0.7565345784590889
2116	75-79	0.17224480121133467
2116	80-84	-0.13958875450065733
2116	85-89	0.1240018659412172
2116	90-94	0.26901814716284633
2116	95-99	0.2534113727572631
2116	100-104	0.17061233484245975
2116	105-109	0.19222506538289963
2116	110-114	0.37644507985564246
2116	115-119	0.26813563121917383
2116	120-124	-0.12179205853813357
2116	125-129	0.03752950758152451
2116	130-134	0.22787072532948827
2116	135-139	0.49521681555498986
2116	140-144	0.49848333611929974
2116	145-149	0.7228175789324922
2116	150-154	1.052598211602458
2116	155-159	-0.1926663908881352
2116	160-164	-0.44720749107124647
2116	165-169	0.6258951157976682
2116	170-174	1.0080288250685356
2116	175-179	0.7291650978570772
2116	180-184	0.8035907383044147
2116	185-189	0.9904819980590389
2116	190-194	0.9802298236686298
2116	195-199	0.9962845738090067
2116	200-204	0.9182624315899517
2116	205-209	0.8938926089182289
2116	210-214	0.7775247622678165
2116	215-219	1.1336284994789665
2116	220-224	1.4753811993641435
2116	225-229	1.3645181769983417
2116	230-234	1.313211973503826
2116	235-239	1.120165798019901
2116	240-244	1.692367333055433
2116	245-249	0.670466398363299
2116	250-254	0.9827992942401629
2116	255-259	0.6591456296376172
2116	260-264	0.07825211776743757
2116	265-269	0.7158004947924717
2116	270-274	0.7778668715884223
2116	275-279	0.8815464098889869
2116	280-284	-0.2966414390499885
2116	285-289	-1.1606415394832972
2116	290-294	0.012923766353857502
2116	295-299	0.2851404522919978
2116	300-301	1.0192960033723182
1117	1	-0.01866192120382948
1117	2	0.04780309728310783
1117	3	0.04574258863898706
1117	4	0.04519641735962665
1117	5	0.022275689407315724
1117	6	-0.304177730603584
1117	7	-0.1257844056626496
1117	8	-0.07297798411060086
1117	9	0.10042038909691797
1117	10-14	0.14407721908889215
1117	15-19	0.12480409179247687
1117	20-24	0.1232740138379782
1117	25-29	0.19002197220549277
1117	30-34	0.13539832972000454
1117	35-39	-0.0756739310070671
1117	40-44	0.03087727128929174
1117	45-49	0.17467927624448265
1117	50-54	0.20802749053654424
1117	55-59	0.2912838641319979
1117	60-64	0.2980970397467715
1117	65-69	0.2995462019862316
1117	70-74	0.05589717969332497
1117	75-79	0.1954528372096931
1117	80-84	0.14588261296244553
1117	85-89	0.3055092721702195
1117	90-94	0.46454814832441826
1117	95-99	0.39076775554794807
1117	100-104	0.384166769236856
1117	105-109	0.2815391874682547
1117	110-114	0.34703732566264733
1117	115-119	0.3629030407813687
1117	120-124	0.536320451499293
1117	125-129	0.4543943975612734
1117	130-134	0.35214891470472054
1117	135-139	0.4595002572167317
1117	140-144	0.47286025838040757
1117	145-149	0.840933927179286
1117	150-154	0.9619119296657814
1117	155-159	-0.019045999895801913
1117	160-164	-0.4409239383034418
1117	165-169	0.42488455567161054
1117	170-174	0.7838324501562965
1117	175-179	0.7759574153779951
1117	180-184	0.6328308233510569
1117	185-189	0.8822631797224929
1117	190-194	0.7537997119060336
1117	195-199	0.6437642338244629
1117	200-204	0.6927221783691451
1117	205-209	1.1443559716946723
1117	210-214	0.8302647892362884
1117	215-219	0.3677946232410143
1117	220-224	0.9364026680852646
1117	225-229	0.34007736875225447
1117	230-234	0.6697003149428546
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2108	165-169	-0.1916559046104993
2108	170-174	-0.7883684230962658
2108	175-179	-0.7882460247840726
2108	180-184	-0.30505525116481635
2108	185-189	-0.9592455087628906
2108	190-194	-0.5097421199110599
2108	195-199	-1.5086087733603648
2108	200-204	-0.602924393422537
2108	205-209	-0.3466477654235902
2108	210-214	0.26956442559077587
2108	215-219	-0.8001537365792935
2108	220-224	-0.2940826539738062
2108	225-229	0.008808887632881124
2108	230-234	0.07000431756009107
2108	235-239	-0.4999633383804216
2108	240-244	-0.25044830167304255
2108	245-249	-0.1462091079974357
2108	250-254	-0.6518115670535067
2108	255-259	-0.5005699628140476
2108	260-264	-0.041634887882281646
2108	265-269	-0.8604307208576287
2108	270-274	-0.7765964052477372
2108	275-279	0.02354494941778995
2108	280-284	-0.34623827213350467
2108	285-289	0.04612582282885924
2108	290-294	-0.1344008810192161
2108	295-299	0.04881545901785245
2108	300-301	-0.34898102495313665
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	27.0
14	133.0
15	275.0
16	377.0
17	495.0
18	1066.0
19	929.0
20	955.0
21	1196.0
22	1753.0
23	2681.0
24	3933.0
25	5589.0
26	7974.0
27	11109.0
28	14884.0
29	21124.0
30	29297.0
31	39861.0
32	52649.0
33	60465.0
34	50651.0
35	20463.0
36	1705.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.712042281938245	23.87643091411633	15.670955742922418	30.740571061023008
2	41.59594899225418	24.571825593072752	18.66370142932126	15.16852398535181
3	40.72124442880244	23.068171130198394	20.37063444145426	15.8399499995449
4	41.26251149130605	22.693167716939172	20.02233030033647	16.021990491418308
5	28.48205535851358	22.819685858791193	32.92111275284666	15.777146029848574
6	27.137989726848243	22.703483344811996	33.46511042272836	16.693416505611395
7	18.03055862328426	44.921358268911035	20.846430175123484	16.20165293268122
8	26.6586164315816	21.934058865827055	34.365907456681946	17.0414172459094
9	16.713744360537262	20.286895815192494	36.06343504340149	26.93592478086876
10-14	25.70065868317976	33.61292730212957	23.812170620485016	16.874243394205653
15-19	21.880174880232524	33.85273531009081	22.71604413875126	21.551045670925404
20-24	24.62273625063335	30.709442136912653	26.717233469964835	17.95058814248916
25-29	19.84829959010549	26.16516533827674	35.60803041289818	18.37850465871959
30-34	20.096602821020824	28.250863933834346	30.5954847889999	21.057048456144926
35-39	20.87397640148424	29.548812640891757	27.60286046292897	21.974350494695035
40-44	20.459227480832652	28.77501919010185	29.113007703346817	21.65274562571868
45-49	21.664456897362534	29.668473924216542	26.232823721089694	22.434245457331226
50-54	22.08230050637597	29.410219146412132	27.531986031426257	20.975494315785642
55-59	21.785999265770016	26.969177510717635	29.799118317217694	21.445704906294658
60-64	22.311550165808548	25.703935412033484	29.541955782364525	22.442558639793443
65-69	20.64047913057461	27.33544298036693	28.590672851998043	23.43340503706041
70-74	21.036356520235316	26.549331456293597	29.16592080631802	23.24839121715307
75-79	20.41905721229259	26.19319957402525	30.28996016347236	23.097783050209802
80-84	22.97988149163979	26.2674720947096	27.628831573102914	23.1238148405477
85-89	21.16669751241668	27.643334132288828	26.350239838348045	24.83972851694645
90-94	20.380221907359594	29.739712436703	28.615551719220743	21.264513936716657
95-99	22.703301304320124	29.432731487240478	26.756493536045532	21.107473672393862
100-104	22.768107719427057	28.01882298685971	26.949092376447602	22.26397691726563
105-109	23.320054490787236	27.568454809964898	24.3793481129986	24.732142586249267
110-114	22.104752167040356	28.180717664299127	28.562274535265797	21.15225563339472
115-119	23.495116763805495	28.227198669890807	26.735376838988216	21.542307727315478
120-124	19.51971650227399	28.313607223366716	27.902802513372393	24.2638737609869
125-129	20.999705701204803	25.279659705640523	30.357679226449267	23.3629553667054
130-134	24.631352833915358	28.630236318898532	26.46268018216185	20.27573066502426
135-139	23.92285123954405	27.921734724527226	25.04810419997755	23.107309835951177
140-144	21.08933030337048	29.33048541097158	26.63319144288328	22.946992842774662
145-149	21.06147810811385	27.36687530529707	28.686486830887414	22.885159755701658
150-154	23.04620491084567	26.048598743313807	26.172871719099383	24.732324626741143
155-159	21.91737182073866	26.95546379366317	29.454090904953624	21.673073480644543
160-164	24.229225387366995	29.722236549483156	25.869349538982455	20.179188524167394
165-169	20.479069894446855	26.397145605087424	28.102379572629605	25.021404927836116
170-174	22.034302496685346	29.652393680767723	25.685488642190315	22.627815180356617
175-179	20.073240957897067	29.615378780753467	28.045097497853437	22.266282763496026
180-184	19.88877325946535	29.941413301698745	26.410434560994183	23.759378877841726
185-189	21.420583318416124	28.473924216543235	27.273427852802058	22.832064612238582
190-194	19.945448532601933	28.56536922362764	25.97523642508882	25.513945818681606
195-199	20.777555620955287	30.50452522322715	26.620812689435887	22.09710646638167
200-204	21.61433508193339	28.656025388580602	26.437497914119362	23.292141615366646
205-209	20.786536285221043	28.668464822191947	27.16760164685965	23.37739724572736
210-214	21.89218955269617	28.723380370573757	26.613591749924908	22.77083832680516
215-219	21.927384047791698	28.247951285964373	26.914079921843946	22.91058474439998
220-224	19.099991808177865	28.575806211828386	26.93786654611541	25.386335433878344
225-229	20.58501746071718	29.851060537565573	25.50818120310561	24.055740798611637
230-234	20.700673853220753	28.11779233427489	26.837926316076903	24.343607496427456
235-239	20.67755471075283	28.805359272080754	24.74069848936732	25.7763875277991
240-244	21.14096912289857	31.94598251804476	23.12521048431873	23.78783787473794
245-249	19.658006595933823	30.842817137291906	23.77309259489619	25.72608367187808
250-254	20.15521985940406	28.796864049126658	24.74179073231856	26.306125359150723
255-259	19.88804509749785	29.722297229647115	25.55872777968246	24.83092989317257
260-264	19.329059427118573	30.901434175675142	25.026259340952738	24.743247056253544
265-269	18.53645512550175	30.602827088838797	25.292948661547282	25.56776912411217
270-274	17.576555611853266	32.33579189130969	23.922123077576558	26.16552941926049
275-279	18.28171979720689	31.421281140301645	23.455007175429387	26.84199188706208
280-284	16.151057200156554	32.62208090486261	24.90489901303713	26.321962881943705
285-289	17.659990837295243	31.432810371453623	25.748474652378512	25.158724138872625
290-294	17.308834728471435	33.652248048374226	23.757983234070696	25.280933989083636
295-299	16.367928106141743	34.60334893824883	21.89140071056472	27.137322245044704
300-301	12.63163803068596	38.146433371662965	25.591707448793528	23.630221148857544
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.25
29	0.25
30	1.5
31	1.0
32	4.5
33	12.25
34	38.5
35	105.5
36	275.75
37	754.0
38	1681.0
39	2544.25
40	4584.25
41	6926.25
42	10320.0
43	14020.5
44	16800.75
45	13940.5
46	10550.5
47	8659.25
48	7238.25
49	4937.0
50	4036.25
51	1641.0
52	338.75
53	86.0
54	41.25
55	33.0
56	24.0
57	14.5
58	11.75
59	9.75
60	5.25
61	4.0
62	2.5
63	2.5
64	1.75
65	1.5
66	1.5
67	1.5
68	1.5
69	0.75
70	0.25
71	0.5
72	0.25
73	0.25
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	3.034008197890151E-4
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	329597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.09797702566118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	35.29334134143621	3.9168469136607706
2	7.297011950091857	1.6196414195618907
3	5.638067119156942	1.8771341807261837
4	6.145625247098465	2.728160312024883
5	5.355613435559757	2.9718237433082257
6	5.399636099551275	3.595502242785048
7	4.153886409824035	3.2269815160403335
8	4.127461836261072	3.6645181306494954
9	3.2732009224289738	3.2693317773740764
>10	22.426608710326942	44.62286293628725
>50	0.7092734278280703	5.090061279084248
>100	0.11765193806354161	2.1681953719501323
>500	0.008168029874824116	0.6242667463734551
>1k	0.04356282599846057	10.757874622888295
>5k	0.008168029874711357	6.142349670928664
>10k+	0.0027226766249037857	3.7244491363570797
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	12326	3.7397185047194	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	7845	2.380179431244823	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	7443	2.2582123016896394	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5040	1.529140131736636	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	4328	1.3131187480468571	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3833	1.1629353422512947	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	3360	1.0194267544910907	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3277	0.9942444864486024	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2842	0.8622651298403808	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	2479	0.7521306322569684	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2287	0.6938776748574775	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2049	0.6216682797476919	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1955	0.5931486026875245	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	1531	0.4645066550969821	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1431	0.4341665731180806	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	1396	0.42354754442546505	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1348	0.4089843050755923	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT	1286	0.3901734542486734	TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1106	0.3355613066866507	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1095	0.33222389766897154	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	894	0.27124033289137944	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	621	0.18841190908897837	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	551	0.1671738517037473	No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	463	0.140474579562314	No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC	458	0.1389575754633689	No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG	423	0.12833854677075338	No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT	343	0.10406648118763218	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	3.0340081978901505E-4	0.0	0.0
9	6.068016395780301E-4	0.0	0.0
10-14	0.0017597247547762873	0.0	0.0
15-19	0.0025485668862277266	0.0	0.0
20-24	0.003276728853721363	0.0	0.0
25-29	0.004308291641004014	0.0	0.0
30-34	0.010801069184488936	0.0	0.0
35-39	0.015170040989450757	0.0	0.0
40-44	0.021359417713146662	0.0	0.0
45-49	0.032281847225551205	0.0	0.0
50-54	0.04508536182064764	0.0	0.0
55-59	0.06741566215711914	0.0	0.0
60-64	0.08525563036071324	0.0	0.0
65-69	0.11432142889650088	0.0	0.0
70-74	0.1569795841588364	0.0	0.0
75-79	0.21517186139436947	0.0	0.0
80-84	0.284954049945843	0.0	0.0
85-89	0.37943306522814224	0.0	0.0
90-94	0.49660646183065993	0.0	0.0
95-99	0.6384766851640032	0.0	0.0
100-104	0.7977014353892784	0.0	0.0
105-109	0.9960648913673366	0.0	0.0
110-114	1.2285305994896798	0.0	0.0
115-119	1.500135013364806	0.0	0.0
120-124	1.8119096957799978	0.0	0.0
125-129	2.177447003461803	0.0	0.0
130-134	2.6124024187113357	0.0	0.0
135-139	3.075513430037288	0.0	0.0
140-144	3.5952997144998284	0.0	0.0
145-149	4.192453208008568	0.0	0.0
150-154	4.908539822874601	0.0	0.0
155-159	5.733183251061144	0.0	0.0
160-164	6.637499734524283	0.0	0.0
165-169	7.612387248670345	0.0	0.0
170-174	8.738853812383002	0.0	0.0
175-179	9.976122355482605	0.0	0.0
180-184	11.298039727303344	0.0	0.0
185-189	12.81200981805053	0.0	0.0
190-194	14.491879477058344	0.0	0.0
195-199	16.196870723944695	0.0	0.0
200-204	17.991911334144426	0.0	0.0
205-209	19.93567902620473	0.0	0.0
210-214	21.98945985552053	0.0	0.0
215-219	24.007196667445395	0.0	0.0
220-224	26.110613870878677	0.0	0.0
225-229	28.303837716969515	0.0	0.0
230-234	30.44390573943331	0.0	0.0
235-239	32.50539294957175	0.0	0.0
240-244	34.44260718392461	0.0	0.0
245-249	36.22806032821901	0.0	0.0
250-254	38.08068641401469	0.0	0.0
255-259	39.80922156451667	0.0	0.0
260-264	41.48053532040644	0.0	0.0
265-269	42.92441982178236	0.0	0.0
270-274	44.20471060112804	0.0	0.0
275-279	45.30138320433742	0.0	0.0
280-284	46.307521002921746	0.0	0.0
285-289	47.232226021474716	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	4260	0.0	292.23004	1
GGGTTAT	4285	0.0	290.8693	2
GGTTATT	4390	0.0	284.2483	3
GTTATTA	4380	0.0	284.22375	4
GGGGGGA	3330	0.0	282.15466	1
TTATTAG	4435	0.0	280.69897	5
TTAGTAG	4490	0.0	276.2751	8
TAGTAGA	4530	0.0	273.83554	9
GGGGAGC	3420	0.0	273.0044	3
GGGAGCG	2105	0.0	269.77435	4
TATTAGT	4625	0.0	268.84866	6
ATTAGTA	4635	0.0	268.26862	7
GGAGCGA	2130	0.0	265.9155	5
AAACGTT	130	0.0	249.61539	295
AACCGTT	30	1.5788828E-9	245.83334	295
CGAAAGC	2415	0.0	233.92339	9
GAGCGAA	2425	0.0	232.95876	6
AGCGAAA	2435	0.0	232.00206	7
GGGGGAG	4180	0.0	224.7787	2
GCGAAAG	2515	0.0	224.62227	8
>>END_MODULE