FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_7637_flu.3510000003b9ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_7637_flu.3510000003b9ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329597
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC123263.7397185047194No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT78452.380179431244823No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG74432.2582123016896394No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA50401.529140131736636No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA43281.3131187480468571No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA38331.1629353422512947No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG33601.0194267544910907No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT32770.9942444864486024No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG28420.8622651298403808No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT24790.7521306322569684No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT22870.6938776748574775No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA20490.6216682797476919No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG19550.5931486026875245No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA15310.4645066550969821No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT14310.4341665731180806No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT13960.42354754442546505No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT13480.4089843050755923No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT12860.3901734542486734TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT11060.3355613066866507No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG10950.33222389766897154No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA8940.27124033289137944No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA6210.18841190908897837No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5510.1671738517037473No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA4630.140474579562314No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC4580.1389575754633689No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG4230.12833854677075338No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT3430.10406648118763218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA42600.0292.230041
GGGTTAT42850.0290.86932
GGTTATT43900.0284.24833
GTTATTA43800.0284.223754
GGGGGGA33300.0282.154661
TTATTAG44350.0280.698975
TTAGTAG44900.0276.27518
TAGTAGA45300.0273.835549
GGGGAGC34200.0273.00443
GGGAGCG21050.0269.774354
TATTAGT46250.0268.848666
ATTAGTA46350.0268.268627
GGAGCGA21300.0265.91555
AAACGTT1300.0249.61539295
AACCGTT301.5788828E-9245.83334295
CGAAAGC24150.0233.923399
GAGCGAA24250.0232.958766
AGCGAAA24350.0232.002067
GGGGGAG41800.0224.77872
GCGAAAG25150.0224.622278
Produced by FastQC (version 0.11.2)