##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_7331_flu.3510000003babc.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	505680
Sequences flagged as poor quality	0
Sequence length	301
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.78374663819016	34.0	34.0	34.0	34.0	34.0
2	33.874740942888785	34.0	34.0	34.0	34.0	34.0
3	33.89357301059959	34.0	34.0	34.0	34.0	34.0
4	33.90220099667774	34.0	34.0	34.0	34.0	34.0
5	33.894263170384434	34.0	34.0	34.0	34.0	34.0
6	37.66433713020092	38.0	38.0	38.0	38.0	38.0
7	37.67865844012024	38.0	38.0	38.0	38.0	38.0
8	37.67403298528714	38.0	38.0	38.0	38.0	38.0
9	37.59610030058535	38.0	38.0	38.0	38.0	38.0
10-14	37.67499723145072	38.0	38.0	38.0	38.0	38.0
15-19	37.64011311501345	38.0	38.0	38.0	37.6	38.0
20-24	37.632564467647526	38.0	38.0	38.0	37.6	38.0
25-29	37.53683831672204	38.0	38.0	38.0	37.0	38.0
30-34	37.47204833096029	38.0	38.0	38.0	37.0	38.0
35-39	37.400633206771076	38.0	38.0	38.0	37.0	38.0
40-44	37.39076570162949	38.0	38.0	38.0	37.0	38.0
45-49	37.36101328903654	38.0	38.0	38.0	37.0	38.0
50-54	37.31666429362442	38.0	38.0	38.0	37.0	38.0
55-59	37.262065733270056	38.0	38.0	38.0	37.0	38.0
60-64	37.092125454833095	38.0	38.0	38.0	36.4	38.0
65-69	37.05243434583136	38.0	38.0	38.0	36.4	38.0
70-74	37.142854374307866	38.0	38.0	38.0	37.0	38.0
75-79	37.04522978959025	38.0	38.0	38.0	37.0	38.0
80-84	36.93490270526816	38.0	38.0	38.0	36.0	38.0
85-89	36.97975913621262	38.0	38.0	38.0	36.0	38.0
90-94	36.83359990507831	38.0	38.0	38.0	35.8	38.0
95-99	36.83206454674893	38.0	38.0	38.0	36.0	38.0
100-104	36.81885540262616	38.0	38.0	38.0	36.0	38.0
105-109	36.655009887676	38.0	38.0	38.0	35.0	38.0
110-114	36.57666745768075	38.0	38.0	38.0	34.8	38.0
115-119	36.63428215472236	38.0	38.0	38.0	34.8	38.0
120-124	36.541419079259605	38.0	37.6	38.0	34.6	38.0
125-129	36.49913186204715	38.0	37.0	38.0	34.0	38.0
130-134	36.3840365448505	38.0	37.0	38.0	34.0	38.0
135-139	36.15131664293625	38.0	37.0	38.0	33.6	38.0
140-144	36.103116199968355	38.0	37.0	38.0	33.4	38.0
145-149	35.66189803828508	38.0	37.0	38.0	30.2	38.0
150-154	35.32919909824395	38.0	36.4	38.0	28.0	38.0
155-159	35.38619126720456	38.0	36.0	38.0	28.8	38.0
160-164	35.33590452460054	38.0	36.0	38.0	28.6	38.0
165-169	35.0572737699731	38.0	35.6	38.0	27.4	38.0
170-174	34.954904287296316	38.0	35.0	38.0	27.6	38.0
175-179	34.68062885619364	38.0	35.0	38.0	25.2	38.0
180-184	34.348349944629014	37.6	34.8	38.0	23.6	38.0
185-189	33.56041330485682	37.0	33.8	37.8	22.2	38.0
190-194	32.61663858566683	36.8	30.8	37.0	20.8	38.0
195-199	32.349555845594054	36.8	30.0	37.2	20.2	37.8
200-204	32.23793861730739	37.0	30.0	37.0	20.0	37.4
205-209	31.108254231925326	35.4	28.0	37.0	18.0	37.2
210-214	30.29903179876602	34.4	26.0	37.0	16.0	37.0
215-219	29.55668327796235	33.2	24.6	37.0	14.4	37.0
220-224	28.07584559405158	31.6	22.0	36.2	9.6	37.0
225-229	26.947526498971683	29.8	20.0	36.0	9.0	37.0
230-234	26.561067077993982	29.0	20.0	35.8	9.0	37.0
235-239	25.73213652903022	28.4	18.4	34.6	9.0	37.0
240-244	24.696393371302012	26.6	17.0	33.8	8.0	37.0
245-249	23.717963534250913	25.0	15.6	33.0	8.0	36.2
250-254	22.929404761904763	24.0	14.2	32.0	8.0	36.0
255-259	22.2774841797184	22.6	13.2	31.2	8.0	36.0
260-264	21.155323129251702	21.2	11.8	29.6	7.8	35.8
265-269	20.251041370036386	19.6	10.4	28.6	7.0	34.6
270-274	19.09548568264515	18.6	9.0	26.8	7.0	33.6
275-279	18.03344328429046	17.0	8.0	25.2	7.0	32.0
280-284	17.75843537414966	16.8	8.0	24.8	7.0	31.0
285-289	16.519767441860466	14.6	8.0	22.6	7.0	29.6
290-294	15.766835152665717	13.4	8.0	21.2	7.0	28.4
295-299	15.080023730422402	13.0	8.0	20.2	7.0	27.0
300-301	13.35420720613827	12.0	8.0	17.0	7.0	23.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.08066495747656433
1101	2	0.001185770715437684
1101	3	0.029773756762267567
1101	4	0.05401649616025139
1101	5	0.06474377944144294
1101	6	0.05673984134220689
1101	7	0.08671301640213613
1101	8	0.05668166681039821
1101	9	0.09133635843703303
1101	10-14	0.04832720816256142
1101	15-19	0.04119884074346203
1101	20-24	0.03630148504245767
1101	25-29	0.07182579605746753
1101	30-34	0.0755463780430432
1101	35-39	0.07673629449002561
1101	40-44	0.057633483854786505
1101	45-49	0.06777780790838506
1101	50-54	0.0780329569509206
1101	55-59	0.08329175829376823
1101	60-64	0.28318264812804017
1101	65-69	0.2663861533953096
1101	70-74	0.2249911866128329
1101	75-79	0.21479105930315257
1101	80-84	0.25004889579466294
1101	85-89	0.1255184106029006
1101	90-94	0.3095409367039039
1101	95-99	0.1686749996820609
1101	100-104	0.18365314796964327
1101	105-109	0.24323027429176847
1101	110-114	0.22595095026547085
1101	115-119	0.24760682146210655
1101	120-124	0.18264506227992428
1101	125-129	0.2668170954410485
1101	130-134	0.2454517160015115
1101	135-139	0.24620932643685478
1101	140-144	0.20289670310594943
1101	145-149	0.4228892491107459
1101	150-154	0.7660739265724672
1101	155-159	0.46487176408342634
1101	160-164	0.3112923956948066
1101	165-169	0.15937762731201843
1101	170-174	0.41303807705577356
1101	175-179	0.5237411677236707
1101	180-184	0.17709655009196013
1101	185-189	0.3136092330585072
1101	190-194	0.4451482455449991
1101	195-199	0.2693322101833928
1101	200-204	-0.09552359808981237
1101	205-209	-0.09716976708784131
1101	210-214	-0.33101426028168035
1101	215-219	-0.3601191779666273
1101	220-224	0.3822971830121489
1101	225-229	0.890528463158553
1101	230-234	0.6407746450646883
1101	235-239	0.6932371931196926
1101	240-244	0.8848966057701801
1101	245-249	0.7793174014595756
1101	250-254	0.5138967701517707
1101	255-259	0.025493541752968696
1101	260-264	0.5869018136092485
1101	265-269	0.35906393178794005
1101	270-274	-0.036340425205878546
1101	275-279	0.5612485070355113
1101	280-284	0.4443958980364897
1101	285-289	0.4788303244390981
1101	290-294	0.4340226212479692
1101	295-299	0.12818386801180282
1101	300-301	0.3893807454481024
1102	1	-0.00957413637600979
1102	2	-0.021882174159919998
1102	3	0.015649825672127804
1102	4	0.021197571916836466
1102	5	0.005925342049835081
1102	6	-0.0015353902755066429
1102	7	0.06750096106834746
1102	8	-0.007545008601780978
1102	9	-0.08468438911868503
1102	10-14	0.04619365855393909
1102	15-19	-0.0030363516704809967
1102	20-24	-0.010176711491133972
1102	25-29	-0.036900412546330585
1102	30-34	0.044312568915678696
1102	35-39	-0.06328077437161284
1102	40-44	-0.08328954766334817
1102	45-49	0.014772543750609657
1102	50-54	-0.07150165803818709
1102	55-59	-0.05995689109915503
1102	60-64	0.05721485587317687
1102	65-69	0.1513000799713211
1102	70-74	0.025851833707434935
1102	75-79	-0.02539315426560762
1102	80-84	0.08217135470999892
1102	85-89	0.10285958391575178
1102	90-94	0.07050204491340395
1102	95-99	0.014093160648748437
1102	100-104	0.016883979051243614
1102	105-109	-0.027959195301193063
1102	110-114	-0.019680891004568934
1102	115-119	-0.06784444891318486
1102	120-124	-0.0183784884499687
1102	125-129	-0.10361252513407493
1102	130-134	0.1133450013390842
1102	135-139	0.06963967961356587
1102	140-144	0.06762580028632925
1102	145-149	0.2532662455372048
1102	150-154	0.609269765906717
1102	155-159	0.45571674245105953
1102	160-164	0.44414089124720135
1102	165-169	0.367756242317391
1102	170-174	0.13751585877768946
1102	175-179	0.3333765343120376
1102	180-184	0.5012216837873922
1102	185-189	0.5363919222814815
1102	190-194	0.6368628179258664
1102	195-199	0.5346320510560503
1102	200-204	0.04039624681476539
1102	205-209	-0.04648140627371333
1102	210-214	-0.5449398486432528
1102	215-219	-0.2022918151522255
1102	220-224	0.5152259684447067
1102	225-229	1.2349152303173199
1102	230-234	0.7313667277388696
1102	235-239	0.870903127959398
1102	240-244	0.9619093574530986
1102	245-249	1.015439249195797
1102	250-254	0.521354782088352
1102	255-259	0.3483968981836867
1102	260-264	0.8886914878194077
1102	265-269	0.7751898811634845
1102	270-274	0.986905877073319
1102	275-279	1.2101926834919894
1102	280-284	0.5232531092819528
1102	285-289	0.7291899731389222
1102	290-294	0.6529008519425261
1102	295-299	0.5646663811431498
1102	300-301	0.45569542251953266
1103	1	-0.02867471941629418
1103	2	-0.001671065467007793
1103	3	0.0017748414222396036
1103	4	0.03222951885902603
1103	5	0.01599452796980927
1103	6	0.04793884255115444
1103	7	0.10929315130671569
1103	8	0.032762089827791385
1103	9	0.09480840019644177
1103	10-14	0.06433136602578315
1103	15-19	0.026386678993546298
1103	20-24	0.028451597462940015
1103	25-29	-0.006642912632230491
1103	30-34	0.09505116297530947
1103	35-39	-0.45778267011164075
1103	40-44	-0.28105509444974786
1103	45-49	-0.10413548708523024
1103	50-54	0.07463446869575563
1103	55-59	-0.34931989954120723
1103	60-64	0.14531716856145493
1103	65-69	0.27626119152788675
1103	70-74	0.10791378030598509
1103	75-79	0.2182919123430267
1103	80-84	0.15986416464345155
1103	85-89	0.19924828507610215
1103	90-94	0.33618628678921425
1103	95-99	0.23256316671970012
1103	100-104	0.24117820248510924
1103	105-109	0.061913429296460265
1103	110-114	0.3059885744082038
1103	115-119	0.08398439611723063
1103	120-124	0.3274186960038179
1103	125-129	0.25210424786968133
1103	130-134	0.395524930549513
1103	135-139	0.4044570356576003
1103	140-144	0.3586720655066671
1103	145-149	0.7007315666675566
1103	150-154	0.8972845652790085
1103	155-159	-0.1103002562087525
1103	160-164	-0.7663083590477484
1103	165-169	0.394467620193943
1103	170-174	0.697370728710446
1103	175-179	0.4629972717606421
1103	180-184	0.5437397534544033
1103	185-189	0.7771983277063939
1103	190-194	0.7228315926248854
1103	195-199	0.5083360177918266
1103	200-204	0.3802157219639639
1103	205-209	0.7854632449903001
1103	210-214	0.48583299473769515
1103	215-219	0.40431979296222664
1103	220-224	1.121338727748725
1103	225-229	1.7506535380113881
1103	230-234	0.937292547603473
1103	235-239	1.1076204622396517
1103	240-244	1.1706260960929775
1103	245-249	1.1421752545107466
1103	250-254	1.3391605345997775
1103	255-259	0.531873376116355
1103	260-264	1.4466361849019158
1103	265-269	0.34961823822697724
1103	270-274	0.5414991807009955
1103	275-279	1.5233915013749417
1103	280-284	0.41682259874942673
1103	285-289	0.49923854073837504
1103	290-294	0.9140322250081248
1103	295-299	0.9391458258230472
1103	300-301	-1.1961811310136454
2116	1	-0.012196481861550978
2116	2	0.007708682499746544
2116	3	0.053104272113095874
2116	4	0.050749940701905416
2116	5	0.006011508933255527
2116	6	-0.2672792287688921
2116	7	-0.12334604790679293
2116	8	0.04164134624033977
2116	9	0.0445268736972011
2116	10-14	0.07361414375391462
2116	15-19	0.09920685952799602
2116	20-24	-0.11519484100341515
2116	25-29	-0.22733198225418505
2116	30-34	-0.0256651655616551
2116	35-39	-0.12959963320037815
2116	40-44	-0.1477415722404345
2116	45-49	0.041247031459761274
2116	50-54	0.17207213068520844
2116	55-59	0.07349447171627332
2116	60-64	0.3256701938541582
2116	65-69	0.22127459755166257
2116	70-74	-0.6953939326263523
2116	75-79	0.17255055922835538
2116	80-84	-0.08324424540213471
2116	85-89	0.18487747445853842
2116	90-94	0.3298675809173659
2116	95-99	0.2285927493454878
2116	100-104	0.08346616387576944
2116	105-109	0.21353957998017137
2116	110-114	0.31882662964100916
2116	115-119	0.2931862908352443
2116	120-124	-0.13371366613756663
2116	125-129	-0.07523018773672874
2116	130-134	0.10177347823027105
2116	135-139	0.3564826858497554
2116	140-144	0.369674389217451
2116	145-149	0.5448570470500727
2116	150-154	0.970262936893981
2116	155-159	-0.42307172819015904
2116	160-164	-0.8113508114563714
2116	165-169	0.5329321836044301
2116	170-174	0.8551073450300422
2116	175-179	0.5699768946045083
2116	180-184	0.6197268836715892
2116	185-189	0.8715646696493664
2116	190-194	0.678903180791842
2116	195-199	0.7449773619691413
2116	200-204	0.5842022265667026
2116	205-209	0.828898762015946
2116	210-214	0.7089963694807473
2116	215-219	1.1793711827200255
2116	220-224	1.232617662416704
2116	225-229	0.7032181103592343
2116	230-234	0.9262777116038308
2116	235-239	1.2116386944319792
2116	240-244	1.5919153087125153
2116	245-249	1.0193206651819402
2116	250-254	1.1023462527157832
2116	255-259	1.0545210658115955
2116	260-264	0.27997070988180894
2116	265-269	0.8078088718850367
2116	270-274	0.8917471921408087
2116	275-279	1.62194320393996
2116	280-284	0.17616302237977877
2116	285-289	-1.0014956624969429
2116	290-294	0.3604957159367146
2116	295-299	0.8940417057050745
2116	300-301	1.465972546705542
1117	1	-0.059443099736917304
1117	2	0.0466042247485845
1117	3	0.021819064750040695
1117	4	0.00529751222552477
1117	5	-0.005603039747157368
1117	6	-0.455151262479248
1117	7	-0.22551060767614928
1117	8	-0.09253918159288332
1117	9	0.05254195464641498
1117	10-14	0.08321693816509423
1117	15-19	0.06333526937496714
1117	20-24	0.012658341520435101
1117	25-29	0.15054337813614183
1117	30-34	0.1024530424667418
1117	35-39	-0.07336144291429747
1117	40-44	-0.05206780865649563
1117	45-49	0.10980702258861896
1117	50-54	0.13897388273665712
1117	55-59	0.21586528564397867
1117	60-64	0.28474005413359293
1117	65-69	0.3167465425505469
1117	70-74	-0.07360639658732282
1117	75-79	0.2229408896785614
1117	80-84	-0.03288984309563148
1117	85-89	0.1324323646781025
1117	90-94	0.2500414553906438
1117	95-99	0.25320108822329246
1117	100-104	0.22077687578528327
1117	105-109	0.24411243427158524
1117	110-114	0.33941145998034017
1117	115-119	0.2855885973333656
1117	120-124	0.3668128364128833
1117	125-129	0.35247240707365535
1117	130-134	0.20510259776897755
1117	135-139	0.36196394555256006
1117	140-144	0.34997002017775003
1117	145-149	0.8188951929804276
1117	150-154	0.9142862236537965
1117	155-159	-0.17255454034363282
1117	160-164	-0.7168699953108799
1117	165-169	0.2750812188672427
1117	170-174	0.6817853224086221
1117	175-179	0.7142476863543337
1117	180-184	0.5343345572133842
1117	185-189	0.9238030817141336
1117	190-194	0.9523285522710978
1117	195-199	0.7706455810864696
1117	200-204	0.8181305920579334
1117	205-209	1.1988894473120233
1117	210-214	0.637784349077112
1117	215-219	0.38545191015404967
1117	220-224	1.1341004745701753
1117	225-229	0.766975262721175
1117	230-234	1.1030625862988828
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2108	160-164	-0.13023834547922775
2108	165-169	-0.09615110436507734
2108	170-174	-0.6128409402176445
2108	175-179	-0.4742694853628322
2108	180-184	-0.2922890118101833
2108	185-189	-0.8743598812488358
2108	190-194	-0.5104970369544901
2108	195-199	-1.452745664704782
2108	200-204	-0.7655677244403769
2108	205-209	-0.3921085324859561
2108	210-214	-0.038479623986862777
2108	215-219	-1.0067716588695212
2108	220-224	-0.5013527240830236
2108	225-229	0.04455910110501193
2108	230-234	-0.18869768306138113
2108	235-239	-0.7250145445446172
2108	240-244	-0.3724436461051255
2108	245-249	-0.3515919096683575
2108	250-254	-0.6822874979646691
2108	255-259	-0.7116913457289655
2108	260-264	0.053741205254699054
2108	265-269	-0.8386352217458821
2108	270-274	-0.6109366401945238
2108	275-279	-0.181968064432386
2108	280-284	-0.32567889378788806
2108	285-289	0.15820787588084428
2108	290-294	-0.36902829059567566
2108	295-299	-0.14367805477396622
2108	300-301	-0.37005494376805714
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	9.0
13	56.0
14	283.0
15	529.0
16	643.0
17	829.0
18	1605.0
19	1458.0
20	1528.0
21	1844.0
22	2668.0
23	3977.0
24	5605.0
25	8065.0
26	11233.0
27	14980.0
28	20806.0
29	29117.0
30	41864.0
31	58836.0
32	78785.0
33	94328.0
34	85008.0
35	38083.0
36	3538.0
37	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.09662236987818	19.916943521594686	15.282787533618098	31.703646574909033
2	46.103069134630594	22.883839582344564	15.850537889574435	15.162553393450404
3	45.95969783262142	21.511627906976745	18.236434108527135	14.292240151874703
4	46.50826609713653	21.408202816010125	17.644360069609238	14.439171017244107
5	30.48014554659073	21.635026103464643	33.6531007751938	14.23172757475083
6	28.597532036070245	22.22611137478247	34.09863945578231	15.077717133364974
7	17.303235247587406	48.49173390286347	19.44510362284449	14.759927226704637
8	28.121341559879763	20.079496915045087	35.643292200601174	16.155869324473976
9	15.863787375415283	18.727060591678534	37.18497864261984	28.224173390286346
10-14	26.953211517165006	34.42540737225123	22.6515187470337	15.969862363550073
15-19	22.271831988609396	35.70016611295681	20.4546748932131	21.573327005220694
20-24	26.72231450719823	29.699335548172755	25.64780098085746	17.930548963771557
25-29	19.533815851922164	26.501305173232083	35.811659547539946	18.153219427305807
30-34	19.966816959341877	28.145902547065337	29.720653377630125	22.166627115962665
35-39	21.382138902072455	29.652705268153774	27.875098876760006	21.090056953013764
40-44	20.907609555450087	30.507356430944473	27.55386805885145	21.031165954753995
45-49	22.153456731529822	28.521792437905397	26.42295522860307	22.901795601961712
50-54	23.330406581237146	30.299912988451194	24.98200442967885	21.387676000632812
55-59	22.46547223540579	27.299477930707166	27.713613352317672	22.52143648156937
60-64	21.258463850656543	27.251937984496124	28.69079259610821	22.798805568739123
65-69	19.00739598164847	29.394360069609238	28.090729314981804	23.507514633760483
70-74	20.96998101566208	26.555212782787535	30.168050941306756	22.306755260243634
75-79	21.82562094605284	25.372211675367822	27.563241575700047	25.23892580287929
80-84	23.69767441860465	27.72721879449454	25.223975636766333	23.35113115013447
85-89	22.055568739123558	27.660575858250276	26.057269419395666	24.2265859832305
90-94	22.83966144597374	29.082186362917263	27.60140800506249	20.47674418604651
95-99	23.84563360227812	27.445024521436483	27.645388387913307	21.063953488372093
100-104	22.915717449770607	27.025272899857615	27.297342192691033	22.761667457680744
105-109	24.813557981332067	28.053077044771396	24.89080050624901	22.242564467647526
110-114	21.0984021515583	27.26483151400095	29.901637399145702	21.735128935295048
115-119	25.384472393608604	26.188380003164056	26.9734614776143	21.453686125613036
120-124	20.877748773928175	27.238648947951273	26.922559721563044	24.961042556557505
125-129	20.47013921847809	24.846820123398196	30.34931181775036	24.333728840373357
130-134	24.0249960449296	28.084124347413386	26.636766334440754	21.254113273216262
135-139	23.461991773453565	25.975597215630437	25.88332542319253	24.679085587723463
140-144	21.985682645151083	27.142540737225122	27.081395348837212	23.790381268786582
145-149	22.895467489321312	25.103148236038603	29.079417813637082	22.921966461003006
150-154	21.74292042398355	27.511825660496758	25.85457206138269	24.890681854137004
155-159	21.840847967093815	26.34420977693403	30.359318145862996	21.45562411010916
160-164	23.323089700996675	29.87529663028002	24.899462110425567	21.90215155829774
165-169	21.85421610504667	28.99418604651163	26.559365606707804	22.592232241733903
170-174	21.91061540895428	30.126008542952064	26.319925644676474	21.64345040341718
175-179	20.0703211517165	28.41769498497073	27.840610662869796	23.671373200442968
180-184	21.739439962031323	28.78729631387439	27.309563360227813	22.163700363866475
185-189	20.653970890681855	27.311026736275906	28.75027685492802	23.28472551811422
190-194	20.646060749881347	25.859476348679006	27.831474450245214	25.66298845119443
195-199	19.379330802088276	31.71733111849391	26.91049675684227	21.992841322575543
200-204	21.083570637557347	27.78785002373042	25.133918683752572	25.994660654959656
205-209	21.482083531086854	26.54856826451511	28.319253282708434	23.650094921689607
210-214	23.059167853187788	26.444945420028475	27.930825818699574	22.565060908084163
215-219	22.18719348204398	27.361335231767125	28.54761904761905	21.903852238569847
220-224	20.127788324632178	27.47749564942256	27.352159468438536	25.042556557506725
225-229	23.066642936244264	27.370550545799716	26.644834678057272	22.91797183989875
230-234	22.35813162474292	27.578033538996994	27.13059642461636	22.93323841164373
235-239	21.433080208827718	27.828508147445024	25.431142224331595	25.307269419395666
240-244	21.956217370669197	29.482716342350894	25.42778041449138	23.13328587248853
245-249	22.330960291093184	27.92572377788325	24.991971207087484	24.751344723936086
250-254	19.902270210409746	27.38882297104889	24.92548647365923	27.78342034488214
255-259	21.187312134155988	28.737739281759218	24.43327796234773	25.641670621737067
260-264	19.366951431735487	28.043980382850815	26.302958392659388	26.28610979275431
265-269	20.2312134155988	28.60239677266255	24.684464483467806	26.481925328270844
270-274	19.188696408796076	30.5124980224648	24.462703686125614	25.83610188261351
275-279	20.032708432210093	29.23947951273533	24.167418130042716	26.560393925011866
280-284	17.382929916152506	30.44973105521278	26.20281601012498	25.964523018509727
285-289	18.682447397563674	29.465867742445816	25.00185888308812	26.849825976902387
290-294	19.85813162474292	30.39712861888942	24.23421926910299	25.51052048726467
295-299	18.624782471127986	30.42596108210726	23.464720772029743	27.48453567473501
300-301	14.7562687865844	32.799497706059164	25.453349944629018	26.99088356272742
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.25
28	0.25
29	1.25
30	1.5
31	2.0
32	7.25
33	34.75
34	152.5
35	346.25
36	643.75
37	1282.5
38	3052.5
39	6910.75
40	9680.75
41	9850.5
42	12017.0
43	15524.5
44	18532.25
45	16986.75
46	16830.5
47	16028.0
48	11835.5
49	9013.75
50	6529.75
51	2701.25
52	970.25
53	692.75
54	697.0
55	754.5
56	831.25
57	840.25
58	1027.75
59	1522.75
60	1711.5
61	984.5
62	353.0
63	91.5
64	32.0
65	12.0
66	12.5
67	12.5
68	13.75
69	9.75
70	8.5
71	7.75
72	9.75
73	10.75
74	10.75
75	9.5
76	5.5
77	3.25
78	0.75
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.25
87	0.0
88	0.0
89	0.25
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	505680.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.38260123765004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.972189968859276	4.2195612526165185
2	7.295880627523715	1.3690867721110191
3	4.748972659197605	1.3367315024926096
4	4.285396936464105	1.6083268239955921
5	3.319083222570385	1.5570817175976188
6	3.3545516122351917	1.8884652065231304
7	3.4160052738914324	2.243571071744297
8	2.75546065967791	2.0682710876631965
9	2.581155621477876	2.1796158535781114
>10	20.952130670919654	38.801517340710234
>50	1.8280409459410494	11.608444599928967
>100	0.4257398358643532	6.194683513580749
>500	0.016875346238147806	1.1077487493708527
>1k	0.03164127335174483	7.287153900240652
>5k	0.012656509340697916	9.195284233824397
>10k+	0.004218836446899305	7.334456374022051
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	20139	3.9825581395348837	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	16919	3.345791805094131	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	8775	1.7352871381110584	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	8679	1.7163028001898433	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	8244	1.630280018984338	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	7833	1.5490033222591362	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	6861	1.3567869008068345	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	6068	1.1999683594367978	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA	4320	0.8542952064546749	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	3903	0.7718319886093973	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	3694	0.7305015029267521	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	3643	0.7204160734061067	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	2634	0.5208827717133365	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	2585	0.5111928492327164	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	2440	0.4825185888308812	No Hit
GGGGGGAGCGAAAGCAGGGGAAAAACTAAAGCAACAAAAATGAAGGCAAT	2232	0.4413858566682487	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	2108	0.41686442018667935	No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	1881	0.3719743711438064	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT	1815	0.35892263882297104	TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	1729	0.34191583610188264	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	1588	0.31403258978009807	No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	1236	0.24442335073564309	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	1011	0.19992880873279545	No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA	977	0.19320518905236514	No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	911	0.18015345673152983	No Hit
GGGGGGAGCAAAAGCAGGGGAAAAACTAAAGCAACAAAAATGAAGGCAAT	787	0.15563202024996045	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTCAACTGTCATACTCCT	667	0.1319015978484417	No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG	650	0.12853978800822655	No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT	559	0.11054421768707484	No Hit
GGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGT	528	0.10441385856668249	No Hit
GGGGGGAGCAATTGGGACAGAAATTTGAGGAAATAAGGTGGTTAATTGAA	518	0.10243632336655592	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	8.305647840531562E-4	0.0	0.0
15-19	0.0015820281601012498	0.0	0.0
20-24	0.002135738016136687	0.0	0.0
25-29	0.002293940832146812	0.0	0.0
30-34	0.0075937351684859985	0.0	0.0
35-39	0.008503401360544218	0.0	0.0
40-44	0.009492168960607499	0.0	0.0
45-49	0.010560037968675842	0.0	0.0
50-54	0.012260718240784684	0.0	0.0
55-59	0.01752096187312134	0.0	0.0
60-64	0.025312450561619997	0.0	0.0
65-69	0.035872488530295836	0.0	0.0
70-74	0.05216737857933871	0.0	0.0
75-79	0.07621420661287771	0.0	0.0
80-84	0.11006960923904445	0.0	0.0
85-89	0.14768232874545167	0.0	0.0
90-94	0.19739756367663344	0.0	0.0
95-99	0.2625375731688024	0.0	0.0
100-104	0.3426672994779307	0.0	0.0
105-109	0.4343062806517956	0.0	0.0
110-114	0.5333807941781364	0.0	0.0
115-119	0.6440436639772188	0.0	0.0
120-124	0.7692611928492327	0.0	0.0
125-129	0.9258424299952539	0.0	0.0
130-134	1.1033064388546117	0.0	0.0
135-139	1.2983309602910933	0.0	0.0
140-144	1.5170859041290934	0.0	0.0
145-149	1.7920819490586932	0.0	0.0
150-154	2.0989558614143333	0.0	0.0
155-159	2.47733744660655	0.0	0.0
160-164	2.904564151241892	0.0	0.0
165-169	3.430351210251542	0.0	0.0
170-174	4.000988767600063	0.0	0.0
175-179	4.649897168169593	0.0	0.0
180-184	5.362719506407214	0.0	0.0
185-189	6.1730738807150765	0.0	0.0
190-194	7.108368928966935	0.0	0.0
195-199	8.127155513368137	0.0	0.0
200-204	9.282747982914096	0.0	0.0
205-209	10.60844803037494	0.0	0.0
210-214	12.141433317513052	0.0	0.0
215-219	13.481964878974846	0.0	0.0
220-224	14.924102199019142	0.0	0.0
225-229	16.44565733270052	0.0	0.0
230-234	18.126206296472077	0.0	0.0
235-239	19.68371302009176	0.0	0.0
240-244	21.208274007277332	0.0	0.0
245-249	22.66077361177029	0.0	0.0
250-254	24.12169751621579	0.0	0.0
255-259	25.506288561936405	0.0	0.0
260-264	26.898591994937505	0.0	0.0
265-269	28.224133839582343	0.0	1.1865211200759373E-4
270-274	29.55457997152349	0.0	1.9775352001265623E-4
275-279	30.593181458629964	0.0	1.9775352001265623E-4
280-284	31.524442335073566	0.0	1.9775352001265623E-4
285-289	32.40060908084164	0.0	3.9550704002531246E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	7795	0.0	291.9724	1
GGGTTAT	7920	0.0	287.36426	2
GGGGGGA	6280	0.0	282.082	1
ATTAGTA	8155	0.0	278.72165	7
TATTAGT	8315	0.0	273.53577	6
GGTTATT	8310	0.0	273.34537	3
TTATTAG	8340	0.0	272.53897	5
GTTATTA	8645	0.0	262.92368	4
TAGTAGA	8655	0.0	262.61987	9
TTAGTAG	8735	0.0	260.72125	8
GGAGCGA	5175	0.0	259.37198	5
GGGGGAG	6900	0.0	256.30798	2
GAGCGAA	5250	0.0	255.94762	6
GGGAGCG	5680	0.0	236.5713	4
GGGGAGC	7570	0.0	233.0383	3
CGAAAGC	5760	0.0	233.02953	9
GCGAAAG	5775	0.0	232.42426	8
AGCGAAA	6240	0.0	215.81331	7
TCGTAAA	20	0.006736589	147.49998	295
TCGAACC	1755	0.0	137.83475	295
>>END_MODULE