FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_7331_flu.3510000003babc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_7331_flu.3510000003babc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505680
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA201393.9825581395348837No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC169193.345791805094131No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC87751.7352871381110584No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG86791.7163028001898433No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG82441.630280018984338No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA78331.5490033222591362No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG68611.3567869008068345No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG60681.1999683594367978No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA43200.8542952064546749No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG39030.7718319886093973No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC36940.7305015029267521No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA36430.7204160734061067No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC26340.5208827717133365No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG25850.5111928492327164No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT24400.4825185888308812No Hit
GGGGGGAGCGAAAGCAGGGGAAAAACTAAAGCAACAAAAATGAAGGCAAT22320.4413858566682487No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG21080.41686442018667935No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA18810.3719743711438064No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT18150.35892263882297104TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC17290.34191583610188264No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA15880.31403258978009807No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC12360.24442335073564309No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT10110.19992880873279545No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA9770.19320518905236514No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA9110.18015345673152983No Hit
GGGGGGAGCAAAAGCAGGGGAAAAACTAAAGCAACAAAAATGAAGGCAAT7870.15563202024996045No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTCAACTGTCATACTCCT6670.1319015978484417No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG6500.12853978800822655No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT5590.11054421768707484No Hit
GGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGT5280.10441385856668249No Hit
GGGGGGAGCAATTGGGACAGAAATTTGAGGAAATAAGGTGGTTAATTGAA5180.10243632336655592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA77950.0291.97241
GGGTTAT79200.0287.364262
GGGGGGA62800.0282.0821
ATTAGTA81550.0278.721657
TATTAGT83150.0273.535776
GGTTATT83100.0273.345373
TTATTAG83400.0272.538975
GTTATTA86450.0262.923684
TAGTAGA86550.0262.619879
TTAGTAG87350.0260.721258
GGAGCGA51750.0259.371985
GGGGGAG69000.0256.307982
GAGCGAA52500.0255.947626
GGGAGCG56800.0236.57134
GGGGAGC75700.0233.03833
CGAAAGC57600.0233.029539
GCGAAAG57750.0232.424268
AGCGAAA62400.0215.813317
TCGTAAA200.006736589147.49998295
TCGAACC17550.0137.83475295
Produced by FastQC (version 0.11.2)