##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_7153_flu.3510000003ba29.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	279927
Sequences flagged as poor quality	0
Sequence length	301
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.80557431044522	34.0	34.0	34.0	34.0	34.0
2	33.881594129898154	34.0	34.0	34.0	34.0	34.0
3	33.8910215877711	34.0	34.0	34.0	34.0	34.0
4	33.89192896719502	34.0	34.0	34.0	34.0	34.0
5	33.89014635958661	34.0	34.0	34.0	34.0	34.0
6	37.645425414483064	38.0	38.0	38.0	38.0	38.0
7	37.63917021223391	38.0	38.0	38.0	38.0	38.0
8	37.654781425157275	38.0	38.0	38.0	38.0	38.0
9	37.57971542580744	38.0	38.0	38.0	37.0	38.0
10-14	37.66698603564501	38.0	38.0	38.0	38.0	38.0
15-19	37.6383292787048	38.0	38.0	38.0	37.6	38.0
20-24	37.63567358632786	38.0	38.0	38.0	37.6	38.0
25-29	37.53704072847563	38.0	38.0	38.0	37.0	38.0
30-34	37.46408099254448	38.0	38.0	38.0	37.0	38.0
35-39	37.38725453421785	38.0	38.0	38.0	37.0	38.0
40-44	37.39567601553262	38.0	38.0	38.0	37.0	38.0
45-49	37.36074119323966	38.0	38.0	38.0	37.0	38.0
50-54	37.32658800330086	38.0	38.0	38.0	37.0	38.0
55-59	37.26796986357157	38.0	38.0	38.0	37.0	38.0
60-64	37.10987293115706	38.0	38.0	38.0	36.6	38.0
65-69	37.036120845791935	38.0	38.0	38.0	36.4	38.0
70-74	37.12045068892961	38.0	38.0	38.0	37.0	38.0
75-79	37.056298963658385	38.0	38.0	38.0	36.8	38.0
80-84	36.96292676304894	38.0	38.0	38.0	36.0	38.0
85-89	37.011145048530516	38.0	38.0	38.0	36.0	38.0
90-94	36.852950947925706	38.0	38.0	38.0	35.8	38.0
95-99	36.86265919328968	38.0	38.0	38.0	36.0	38.0
100-104	36.81707945285736	38.0	38.0	38.0	35.6	38.0
105-109	36.65969270559825	38.0	38.0	38.0	35.0	38.0
110-114	36.58972660729405	38.0	38.0	38.0	34.6	38.0
115-119	36.660722259732	38.0	38.0	38.0	34.8	38.0
120-124	36.56608687264894	38.0	37.8	38.0	34.6	38.0
125-129	36.4768678977019	38.0	37.0	38.0	34.0	38.0
130-134	36.35757822575171	38.0	37.0	38.0	34.0	38.0
135-139	36.080925384118004	38.0	37.0	38.0	33.2	38.0
140-144	36.04173016536454	38.0	37.0	38.0	32.8	38.0
145-149	35.604578336494875	38.0	36.8	38.0	29.8	38.0
150-154	35.22680770343697	38.0	36.2	38.0	28.0	38.0
155-159	35.327416790806176	38.0	36.0	38.0	28.6	38.0
160-164	35.232898577129035	38.0	35.8	38.0	28.2	38.0
165-169	34.92282059251162	38.0	35.2	38.0	26.2	38.0
170-174	34.86098661436732	38.0	35.0	38.0	26.0	38.0
175-179	34.50857902238798	38.0	35.0	38.0	24.0	38.0
180-184	34.22488648826301	37.6	34.6	38.0	23.0	38.0
185-189	33.37277361597845	37.0	33.4	37.8	21.8	38.0
190-194	32.3802991494211	36.8	30.2	37.0	20.2	38.0
195-199	32.09045858384508	36.8	29.8	37.2	20.0	37.8
200-204	31.947913563178975	36.8	29.8	37.0	19.8	37.4
205-209	30.752701954438123	35.0	26.8	37.0	16.6	37.2
210-214	29.933432644939575	33.8	25.2	37.0	15.4	37.0
215-219	29.096867397571508	32.8	23.6	36.8	13.2	37.0
220-224	27.5583462831381	31.0	21.0	36.2	9.4	37.0
225-229	26.512167815180383	29.2	19.6	35.8	9.0	37.0
230-234	26.12335144519821	28.8	19.2	35.6	9.0	37.0
235-239	25.06137600160042	27.2	17.8	33.8	8.6	37.0
240-244	24.072695381295837	25.6	16.2	33.0	8.0	37.0
245-249	23.09403808850164	24.0	15.0	32.0	8.0	36.2
250-254	22.15470176153068	22.4	13.0	31.2	8.0	36.0
255-259	21.56880472408878	21.4	12.4	30.2	8.0	36.0
260-264	20.315006412386087	19.8	11.0	28.4	7.2	34.8
265-269	19.45679695063356	19.0	9.8	27.2	7.0	33.8
270-274	18.544625563093234	17.8	8.2	26.2	7.0	32.6
275-279	17.334948754496708	16.0	8.0	24.0	7.0	31.0
280-284	17.093808028521725	15.8	8.0	23.8	7.0	30.4
285-289	15.93372915081432	13.4	8.0	21.6	7.0	29.0
290-294	15.334038517184837	12.8	8.0	20.6	7.0	28.0
295-299	14.554666752403303	12.2	8.0	19.2	7.0	27.0
300-301	12.89567280040868	10.0	8.0	16.0	7.0	22.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.06147198674500487
1101	2	0.08153162929637148
1101	3	0.07784420101319256
1101	4	0.06401040516315248
1101	5	0.05986901922133825
1101	6	0.15731052489967823
1101	7	0.17614925754116229
1101	8	0.0368939333935856
1101	9	0.11131179548721093
1101	10-14	0.05732780512120428
1101	15-19	0.06943975783990908
1101	20-24	0.06091109511769588
1101	25-29	0.05641589493074406
1101	30-34	0.12821047975340605
1101	35-39	0.16544792303054834
1101	40-44	0.19577473490083008
1101	45-49	0.2206494327097417
1101	50-54	0.13975998481500795
1101	55-59	0.19728305140814228
1101	60-64	0.3181893563935674
1101	65-69	0.3138537369245924
1101	70-74	0.2695290608344578
1101	75-79	0.22088350618382435
1101	80-84	0.41500586335050826
1101	85-89	0.22194207211178707
1101	90-94	0.43651843970129534
1101	95-99	0.2873360469857573
1101	100-104	0.2196786469801424
1101	105-109	0.342441753344751
1101	110-114	0.44417025327301474
1101	115-119	0.27536404513485735
1101	120-124	0.322712650277424
1101	125-129	0.3802987264181894
1101	130-134	0.3967681642266072
1101	135-139	0.27158617204764823
1101	140-144	0.31181339562193955
1101	145-149	0.4927657183974219
1101	150-154	0.9224652953532129
1101	155-159	0.6285489062814946
1101	160-164	0.2755108567548774
1101	165-169	0.27366097569534276
1101	170-174	0.5110173168903955
1101	175-179	0.6299296638849299
1101	180-184	0.31245938976731935
1101	185-189	0.27762867737109076
1101	190-194	0.2930596271440038
1101	195-199	-0.12306107369587949
1101	200-204	-0.04637587576398161
1101	205-209	-0.2623461657237556
1101	210-214	-0.5170712623899654
1101	215-219	-0.16423221988369718
1101	220-224	0.22514402187719185
1101	225-229	0.5681982048185397
1101	230-234	0.18095674905839587
1101	235-239	0.36073209370621484
1101	240-244	0.5887369483592124
1101	245-249	0.03672826868976031
1101	250-254	-0.16616542650028165
1101	255-259	-0.4310276951628538
1101	260-264	-0.07528439579797563
1101	265-269	-0.009378604345293695
1101	270-274	0.2147104824819408
1101	275-279	0.1595346561962856
1101	280-284	0.008455361033576025
1101	285-289	-0.08557760315387064
1101	290-294	-0.21590941962269206
1101	295-299	0.29769533731077225
1101	300-301	-0.07036377734774035
1102	1	-0.16440034851348884
1102	2	-0.065935578555397
1102	3	0.005254309250211975
1102	4	0.02043086318309406
1102	5	-0.03617987089056385
1102	6	0.04086973421907203
1102	7	0.13627889736260101
1102	8	0.039827263669380386
1102	9	-0.04964299874014699
1102	10-14	0.03566243791028967
1102	15-19	0.004520859166881053
1102	20-24	0.034143070901954786
1102	25-29	0.006598200929637699
1102	30-34	0.07847763256939544
1102	35-39	0.021383760491573867
1102	40-44	0.02276825290975637
1102	45-49	0.025582013887508026
1102	50-54	0.12778779316820277
1102	55-59	-0.09035721337507141
1102	60-64	0.10462686021549672
1102	65-69	0.1972852938254661
1102	70-74	0.05471602958637334
1102	75-79	-0.062297332194454214
1102	80-84	-0.033148527281177564
1102	85-89	0.003441644055776294
1102	90-94	0.1518367299232679
1102	95-99	-0.12999295992428728
1102	100-104	-0.06637054888606286
1102	105-109	-0.10244106632137573
1102	110-114	0.12092757608839833
1102	115-119	0.05258955421577838
1102	120-124	0.06618059193951353
1102	125-129	0.010830586015813992
1102	130-134	0.020233277661453997
1102	135-139	0.15897074980096448
1102	140-144	0.25036480820678975
1102	145-149	0.2815613522260847
1102	150-154	0.6232766908158212
1102	155-159	0.4094323833193414
1102	160-164	0.5032756399750866
1102	165-169	0.5239713163056408
1102	170-174	0.6361526131663098
1102	175-179	0.3852088939956104
1102	180-184	0.3938997218164815
1102	185-189	0.4103615857059566
1102	190-194	0.8193067224781956
1102	195-199	0.6422725042408928
1102	200-204	0.041277001383942746
1102	205-209	-0.09158124491411712
1102	210-214	-0.28619542920950636
1102	215-219	-0.026946247890794695
1102	220-224	0.7866885397026699
1102	225-229	1.0529181338835372
1102	230-234	0.6929304694766714
1102	235-239	1.0750333687016287
1102	240-244	1.1134537434426797
1102	245-249	1.24600752937587
1102	250-254	0.6513860166599983
1102	255-259	0.46180733510681904
1102	260-264	0.7149169434058358
1102	265-269	0.7774590554113239
1102	270-274	0.5207800874473882
1102	275-279	0.8078423061687694
1102	280-284	0.5717017837083169
1102	285-289	0.8805272399941799
1102	290-294	0.39333582589800464
1102	295-299	0.7554500306392136
1102	300-301	0.4187179278439661
1103	1	-0.025779674545312048
1103	2	-0.052964591259112126
1103	3	-0.006863697732846674
1103	4	-0.025967474832157222
1103	5	0.00907357799316344
1103	6	0.12516693530633916
1103	7	0.08860677541294848
1103	8	0.04214926563312815
1103	9	0.0719389195936202
1103	10-14	0.018892465850136375
1103	15-19	-0.020094257160678808
1103	20-24	0.04202119707480989
1103	25-29	0.016530303696704607
1103	30-34	-0.014799745255388075
1103	35-39	-0.4290330625548293
1103	40-44	-0.31972684719222144
1103	45-49	-0.2225226638565232
1103	50-54	0.040896015519329865
1103	55-59	-0.3495593545452067
1103	60-64	0.1468501439243326
1103	65-69	0.21716807537531224
1103	70-74	0.06546396063523474
1103	75-79	0.2105142046486037
1103	80-84	0.2339506951798782
1103	85-89	0.16985165842375238
1103	90-94	0.26862906902275085
1103	95-99	0.06532664232151575
1103	100-104	0.18592586366828812
1103	105-109	-0.0020612057552185092
1103	110-114	0.2611704290611385
1103	115-119	0.009507224555939331
1103	120-124	0.2786784594887237
1103	125-129	0.2652556583295933
1103	130-134	0.34868718450083946
1103	135-139	0.20960328209100965
1103	140-144	0.31479666645283544
1103	145-149	0.5871375688603351
1103	150-154	0.764143925377823
1103	155-159	-0.20482300277745935
1103	160-164	-0.6274672869226023
1103	165-169	0.44301817714853087
1103	170-174	0.6945738034719042
1103	175-179	0.5587479282702645
1103	180-184	0.7034553759386526
1103	185-189	0.7557613681012043
1103	190-194	0.3487485700714643
1103	195-199	0.36217272449936644
1103	200-204	0.37073856230000146
1103	205-209	0.9454229365849294
1103	210-214	0.7001515783460022
1103	215-219	0.3166054104924889
1103	220-224	1.2878637570231
1103	225-229	1.9883698912125354
1103	230-234	0.95592818348943
1103	235-239	1.2118798436183233
1103	240-244	1.0436026169400172
1103	245-249	1.234421782108253
1103	250-254	1.3013130394554224
1103	255-259	0.42547719760639424
1103	260-264	1.5992277921781195
1103	265-269	0.8675675165969281
1103	270-274	0.6801717797982398
1103	275-279	1.1529103739977629
1103	280-284	0.47793195191603033
1103	285-289	0.9046324636456156
1103	290-294	0.8171665794397711
1103	295-299	1.0506559021765884
1103	300-301	-0.8371790093880538
2116	1	-0.10239313140056083
2116	2	-0.029273097361745215
2116	3	-0.019683191456792315
2116	4	0.014940729210245252
2116	5	-0.023889285942431115
2116	6	-0.22752089484620797
2116	7	-0.11910659978487104
2116	8	0.09877833134206782
2116	9	0.09519322106128669
2116	10-14	0.04982328827774296
2116	15-19	0.08089141304447622
2116	20-24	-0.11874999591855584
2116	25-29	-0.2752085659621386
2116	30-34	-0.0016232712665313898
2116	35-39	-0.21030027023206088
2116	40-44	-0.2737118019915812
2116	45-49	0.03576628782106184
2116	50-54	0.16711238602727008
2116	55-59	0.16324135074373203
2116	60-64	0.30877538040568453
2116	65-69	0.14043827474952053
2116	70-74	-0.7221963733653283
2116	75-79	0.16803716628407273
2116	80-84	-0.03392830729874419
2116	85-89	0.15542936666826535
2116	90-94	0.23362765989012502
2116	95-99	0.2597869445262617
2116	100-104	0.20894458706639796
2116	105-109	0.164914506579386
2116	110-114	0.4188220192130956
2116	115-119	0.14501158564075212
2116	120-124	-0.18576225938570445
2116	125-129	-0.04329813801822979
2116	130-134	0.13457851508335494
2116	135-139	0.30341971908109855
2116	140-144	0.398349559510514
2116	145-149	0.47601842850350096
2116	150-154	0.9031747311848974
2116	155-159	-0.3660935345624239
2116	160-164	-0.6682107308427447
2116	165-169	0.5974836823045067
2116	170-174	0.706367882224356
2116	175-179	0.4819921128883138
2116	180-184	0.48189420151692985
2116	185-189	0.7276162924777481
2116	190-194	0.6487370079576138
2116	195-199	0.7221656427046312
2116	200-204	0.5697521694627277
2116	205-209	0.469925223715574
2116	210-214	0.5246162886066514
2116	215-219	0.9779070154584169
2116	220-224	1.3319761118643747
2116	225-229	0.7863559155074284
2116	230-234	1.2017538990758858
2116	235-239	0.9699180419519351
2116	240-244	1.5008690441745784
2116	245-249	0.647292582712371
2116	250-254	0.6998511546863426
2116	255-259	0.47282983485229835
2116	260-264	0.10507316335809946
2116	265-269	0.9533873066000353
2116	270-274	1.0907879682028891
2116	275-279	1.4715203188609358
2116	280-284	0.33774854788519093
2116	285-289	-0.9314760178875225
2116	290-294	0.4132737602350982
2116	295-299	0.7275808134734962
2116	300-301	1.4848710256596025
1117	1	-0.09338400892183785
1117	2	0.0403312353658265
1117	3	0.020421528121453036
1117	4	0.01347969317016151
1117	5	-0.020044170157127894
1117	6	-0.1759456029088966
1117	7	-0.07812561844366428
1117	8	0.0648794163232651
1117	9	0.11624869364887047
1117	10-14	0.050510982827653095
1117	15-19	0.09044464549675268
1117	20-24	0.04356283133012795
1117	25-29	0.1114593002415063
1117	30-34	0.08972674762618027
1117	35-39	-0.14456192523323352
1117	40-44	-0.09798803136705203
1117	45-49	0.06125273005436327
1117	50-54	0.041591397128975416
1117	55-59	0.1801882892254838
1117	60-64	0.14127587521469565
1117	65-69	0.13075153383149996
1117	70-74	-0.0974836324833035
1117	75-79	0.12861366375604888
1117	80-84	0.03579190070095706
1117	85-89	0.009892903742958481
1117	90-94	0.1999266684728127
1117	95-99	0.14210588941354274
1117	100-104	0.046837240502156874
1117	105-109	0.23152659431352163
1117	110-114	0.16701749575915414
1117	115-119	0.18771486217601563
1117	120-124	0.31571928873672306
1117	125-129	0.23594579382407943
1117	130-134	0.17929953277052846
1117	135-139	0.2787177740318896
1117	140-144	0.3380152485545409
1117	145-149	0.7548272882836287
1117	150-154	0.9377516245040738
1117	155-159	-0.3068107377220173
1117	160-164	-0.8406162223230282
1117	165-169	0.3098952914233877
1117	170-174	0.6225587525484073
1117	175-179	0.898889030678049
1117	180-184	0.6074641315239475
1117	185-189	0.8373087911732569
1117	190-194	0.9837734438933481
1117	195-199	0.7793083456213097
1117	200-204	0.6754340162698647
1117	205-209	1.2379853924904296
1117	210-214	0.6847663506826898
1117	215-219	0.281905072208513
1117	220-224	0.9834085389475185
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2108	165-169	-0.20908521778580536
2108	170-174	-0.4414496836306441
2108	175-179	-0.7123368069076363
2108	180-184	-0.30525358713305195
2108	185-189	-0.8537479653661251
2108	190-194	-0.17817474152386126
2108	195-199	-1.4893610794852634
2108	200-204	-0.6409866845406853
2108	205-209	-0.33862887573359757
2108	210-214	-0.01301218174387131
2108	215-219	-0.8580354098333274
2108	220-224	-0.5822526376492334
2108	225-229	-0.10954017299655305
2108	230-234	-0.32067838959730466
2108	235-239	-0.7675247956586908
2108	240-244	-0.24578260527941254
2108	245-249	0.12344127453703635
2108	250-254	-0.2760555006043681
2108	255-259	-0.6170242649538586
2108	260-264	-0.034778259052764326
2108	265-269	-0.7045383189287477
2108	270-274	-0.5194696686209355
2108	275-279	-0.23445741234999673
2108	280-284	-0.21194533658698944
2108	285-289	0.24376949926030278
2108	290-294	-0.2480585830569524
2108	295-299	-0.08413975213518299
2108	300-301	-0.234734290576478
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	39.0
14	137.0
15	267.0
16	336.0
17	427.0
18	691.0
19	722.0
20	869.0
21	1121.0
22	1636.0
23	2451.0
24	3469.0
25	5123.0
26	6976.0
27	9408.0
28	12864.0
29	17785.0
30	25147.0
31	34801.0
32	44661.0
33	51022.0
34	41895.0
35	16675.0
36	1399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.98447809607505	20.896876685707344	13.782164635780042	30.336480582437563
2	48.29294780424897	21.53418569841423	16.274242927620417	13.89862356971639
3	47.47487737874517	20.41996663415819	17.74784140150825	14.357314585588385
4	47.79889042500366	20.136678491178056	17.438475030990222	14.625956052828059
5	33.313328117687824	20.252422953127066	32.004772672875426	14.42947625630968
6	32.09193825533085	20.575721527398212	32.354506710678145	14.977833506592791
7	16.964780103384097	49.74475488252295	18.55519474720195	14.73527026689101
8	31.556441500819854	19.280741050345267	33.26367231456773	15.899145134267147
9	15.369006919661196	18.090787955431235	34.755847060126385	31.784358064781177
10-14	27.265608533653417	35.734459341185385	21.320487127000966	15.679444998160236
15-19	22.902471001368212	34.622669481686295	20.190406784625992	22.284452732319497
20-24	24.561403508771928	30.080199480578866	26.017068735777542	19.341328274871664
25-29	19.82945553662205	25.068321383789343	36.803952459033965	18.298270620554643
30-34	21.83204906993609	26.963101094213847	28.807653423928382	22.39719641192168
35-39	21.526112164957294	29.010777809929017	27.199376980427044	22.263733044686653
40-44	22.794871520074878	27.37970971003154	28.60267141076066	21.222747359132917
45-49	22.791584948933114	28.832874285081466	25.567022830952357	22.808517935033063
50-54	23.205907254391324	27.918493035684307	27.36970710220879	21.50589260771558
55-59	22.28702483147392	26.634086744043984	27.33862757075952	23.740260853722578
60-64	23.021359140061513	26.159320108456846	27.49016707927424	23.329153672207397
65-69	19.874252930228238	29.19739789302211	27.593622623041007	23.334726553708645
70-74	22.076755725599888	26.20633236522379	28.28801794753632	23.42889396164
75-79	21.114147617057306	24.83668956549386	27.648136835675015	26.401025981773817
80-84	26.66302285953123	23.792774544792035	26.733898480675318	22.81030411500141
85-89	21.698085572309925	28.736563461188098	25.085182922690556	24.480168043811425
90-94	22.01052417237351	29.543773912484326	27.417576725360544	21.028125189781623
95-99	22.039103051867095	29.39151993198227	26.329936019033536	22.239440997117104
100-104	22.649047787458873	28.782575457172765	24.52003558070497	24.048341174663395
105-109	25.410910701718663	25.60017433116491	23.836214441622282	25.152700525494147
110-114	20.813926487977223	28.005944406934663	29.15260049941592	22.02752860567219
115-119	25.086969102658905	26.87086276064831	24.80989686596863	23.23227127072415
120-124	19.559956702997567	27.170512312138523	28.046597863014284	25.22293312184963
125-129	22.34361101287121	24.134506496336545	30.446652162885325	23.07523032790692
130-134	25.63611227212809	27.87383853647558	24.383785772719317	22.106263418677013
135-139	24.825186566497695	27.106209833277962	24.26246842926906	23.806135170955283
140-144	22.436278029629154	26.972103441254326	26.297070307615915	24.294548221500605
145-149	20.31593951280155	26.544706298427805	28.71498640717044	24.424367781600203
150-154	23.05901181379431	25.03231199562743	26.369946450324548	25.538729740253707
155-159	23.63987753950137	25.279447856048183	28.69290922276165	22.387765381688798
160-164	25.847524533181865	27.538751174413328	24.918782396839177	21.69494189556563
165-169	22.041389362226578	25.287307047908918	27.79617543145177	24.87512815841273
170-174	23.995970377991405	28.196851321951794	25.665334176410276	22.14184412364652
175-179	22.207289757686826	29.17453478942724	26.096946703962104	22.52122874892383
180-184	21.61670721295195	28.37925602031958	26.645875531835085	23.35816123489338
185-189	22.5948193636198	26.488191564229247	27.756665130551895	23.16032394159906
190-194	21.059276168429626	26.723324295262696	26.01749741896995	26.199902117337736
195-199	20.932743179471792	29.147456301107074	28.140479482150702	21.77932103727043
200-204	22.653263172184175	28.23378952369725	25.02788226930593	24.085065034812647
205-209	22.257159902403124	27.050623912662946	27.34134256431141	23.35087362062252
210-214	23.88151196561961	27.440439828955405	25.796153997292155	22.881894208132834
215-219	23.543352374011796	26.443036934629387	27.737017150900055	22.276593540458762
220-224	19.694491778213603	27.813894336737793	27.29926016425711	25.192353720791495
225-229	22.3690462156205	27.965791081246184	25.522011095749964	24.143151607383352
230-234	21.660361451378396	27.202592104370066	27.26024999374837	23.876796450503168
235-239	21.493818031129543	27.289543345229294	24.90020612516835	26.316432498472814
240-244	22.544520535711097	29.569923587220952	23.937097886234625	23.948457990833326
245-249	21.145155701307843	29.04135721098715	23.439253805456424	26.37423328224859
250-254	20.96575178528688	26.9040857080596	24.71151407331197	27.41864843334155
255-259	21.80961464953363	27.681359783086307	25.704058558124082	24.804967009255986
260-264	21.847267323266422	29.05800440829216	24.313838965158773	24.780889303282642
265-269	20.66853143855362	28.828658900356164	25.2929513766089	25.209858284481314
270-274	19.70192228688194	30.145216431426764	24.170444437299725	25.982416844391576
275-279	20.614588803509488	29.71224640709899	22.554594590732584	27.118570198658936
280-284	18.294841155015416	29.999964276400632	25.124979012385374	26.58021555619858
285-289	19.875110296613048	28.591168411764496	25.526798058065136	26.006923233557323
290-294	19.447141576196653	31.179557527498243	24.388144051842087	24.98515684446302
295-299	18.828265940763128	31.98005194211348	22.19721570266534	26.994466414458056
300-301	14.39178785897752	35.12308566161892	25.607211880240204	24.877914599163354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.75
32	1.25
33	3.75
34	13.0
35	30.0
36	81.75
37	300.75
38	975.5
39	1867.75
40	3311.25
41	4889.75
42	8229.75
43	10882.75
44	13080.75
45	10160.0
46	6818.5
47	5797.5
48	5325.25
49	4498.25
50	4041.75
51	2073.25
52	682.5
53	379.5
54	455.75
55	654.75
56	806.0
57	851.5
58	1219.25
59	2093.75
60	2094.0
61	1108.5
62	363.0
63	108.5
64	44.0
65	17.25
66	11.25
67	6.75
68	6.75
69	5.25
70	2.25
71	0.75
72	0.5
73	0.25
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.25
80	0.25
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	279927.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.55325506370794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	38.6459486424931	4.8513245048817355
2	10.581343540695617	2.656606087661411
3	7.6959529969452225	2.898277827868828
4	7.347816877056217	3.6895607767641843
5	6.003396753534185	3.768108534787541
6	4.8048936363607595	3.6190233222734256
7	3.817815503298314	3.3548208259357573
8	3.1638759814320614	3.177355374788476
9	2.444813979315504	2.762133611909968
>10	14.625481684902264	33.683568089007146
>50	0.6557981847005491	5.318216872000294
>100	0.15044770093348908	3.571493624328575
>500	0.01134809424230142	1.1097285125676146
>1k	0.03404428272690426	10.004295136886029
>5k	0.014185117802876774	11.565607652642262
>10k+	0.002837023560575355	3.969879245696791
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	11147	3.9821096214370173	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	7799	2.7860835146306004	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	6802	2.429919228941831	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	6647	2.3745476499230156	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5838	2.085543731044165	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	5389	1.9251447698864346	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	4066	1.4525215502613182	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	3567	1.2742607894200988	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	3250	1.161016979426779	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	2716	0.9702529588071176	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	2575	0.9198826836996788	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2351	0.8398618211176486	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1983	0.7083989754471702	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	1946	0.6951812436813883	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	1742	0.6223051009727536	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1640	0.5858670296184363	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1215	0.43404173230878046	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	1040	0.37152543341656935	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	847	0.3025788866383021	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	838	0.29936376269527415	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	792	0.28293090698646434	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	639	0.22827379995498825	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT	483	0.17254498494250287	TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	465	0.16611473705644686	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	452	0.16147066913873975	No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	416	0.14861017336662774	No Hit
GGGGGGAGCAAAAGCAGAAGAAGTGTCATTCCGGGGGAGGGGAGTTTTCG	412	0.1471812293919486	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	329	0.11753064191735703	No Hit
GGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGT	326	0.1164589339363477	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	297	0.10609909011992412	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTG	284	0.10145502220221701	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	5.715775898716451E-4	0.0	0.0
15-19	0.0010002607822753788	0.0	0.0
20-24	0.0017147327696149354	0.0	0.0
25-29	0.0017861799683488908	0.0	0.0
30-34	0.002572099154422403	0.0	0.0
35-39	0.003072229545560092	0.0	0.0
40-44	0.0038581487316336044	0.0	0.0
45-49	0.00721616707212952	0.0	0.0
50-54	0.017361669292351216	0.0	0.0
55-59	0.02329178678726954	0.0	0.0
60-64	0.027078488320169188	0.0	0.0
65-69	0.03579504656571177	0.0	0.0
70-74	0.047512387158080496	0.0	0.0
75-79	0.061658932507403716	0.0	0.0
80-84	0.08209283134531503	0.0	0.0
85-89	0.10852829487687861	0.0	0.0
90-94	0.14775280698182022	0.0	0.0
95-99	0.19833742368546087	0.0	0.0
100-104	0.26428318811690193	0.0	0.0
105-109	0.3452328642824737	0.0	0.0
110-114	0.4450446009138097	0.0	0.0
115-119	0.5647901059919194	0.0	0.0
120-124	0.715043564929428	0.0	0.0
125-129	0.8960907665212716	0.0	0.0
130-134	1.1106467043193404	0.0	0.0
135-139	1.3709288493071408	0.0	0.0
140-144	1.642999782086044	0.0	0.0
145-149	1.9639405987989726	0.0	0.0
150-154	2.35439953988004	0.0	0.0
155-159	2.818663437253284	0.0	0.0
160-164	3.344943503127601	0.0	0.0
165-169	3.9185216145637964	0.0	0.0
170-174	4.5775505756858035	0.0	0.0
175-179	5.33374772708599	0.0	0.0
180-184	6.220836146566784	0.0	0.0
185-189	7.438296412993386	0.0	0.0
190-194	8.690980148395832	0.0	0.0
195-199	9.869930374704833	0.0	0.0
200-204	11.127043836428783	0.0	0.0
205-209	12.566847785315456	0.0	0.0
210-214	14.096460863010714	0.0	0.0
215-219	15.577918528759284	0.0	0.0
220-224	17.196269027282113	0.0	0.0
225-229	18.944367638705806	0.0	0.0
230-234	20.819213580683538	0.0	0.0
235-239	22.59560528280587	0.0	0.0
240-244	24.352920582866247	0.0	0.0
245-249	26.05393549032426	0.0	0.0
250-254	27.770097203913878	0.0	3.572359936697782E-4
255-259	29.638227109210618	0.0	3.572359936697782E-4
260-264	31.249289993462583	0.0	3.572359936697782E-4
265-269	32.67030332908222	0.0	3.572359936697782E-4
270-274	33.966641302911114	0.0	3.572359936697782E-4
275-279	35.10208018519114	0.0	3.572359936697782E-4
280-284	36.11362962486648	0.0	3.572359936697782E-4
285-289	37.05223147463446	0.0	3.572359936697782E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	4015	0.0	294.26526	1
GGGTTAT	4035	0.0	292.80667	2
GTTATTA	4090	0.0	288.14792	4
GGGGGGA	4470	0.0	287.74048	1
GGTTATT	4130	0.0	285.71426	3
TTATTAG	4180	0.0	281.5909	5
TTAGTAG	4185	0.0	280.5496	8
GGGGAGC	4645	0.0	276.8999	3
TATTAGT	4265	0.0	275.97888	6
GGGAGCG	3555	0.0	275.0844	4
GGAGCGA	3560	0.0	274.69806	5
ATTAGTA	4300	0.0	273.73254	7
CGAAAGC	3590	0.0	271.58078	9
GCGAAAG	3710	0.0	262.7965	8
AGCGAAA	3715	0.0	262.4428	7
GAGCGAA	3780	0.0	258.3201	6
TAGTAGA	4610	0.0	254.36551	9
GGGGGAG	5270	0.0	244.6205	2
GGGAGCA	1285	0.0	241.05058	4
AAACGTT	125	0.0	200.59999	295
>>END_MODULE