FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_7153_flu.3510000003ba29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_7153_flu.3510000003ba29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279927
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC111473.9821096214370173No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG77992.7860835146306004No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT68022.429919228941831No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG66472.3745476499230156No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA58382.085543731044165No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG53891.9251447698864346No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC40661.4525215502613182No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA35671.2742607894200988No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA32501.161016979426779No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT27160.9702529588071176No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT25750.9198826836996788No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA23510.8398618211176486No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG19830.7083989754471702No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC19460.6951812436813883No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA17420.6223051009727536No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT16400.5858670296184363No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT12150.43404173230878046No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA10400.37152543341656935No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG8470.3025788866383021No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT8380.29936376269527415No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA7920.28293090698646434No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT6390.22827379995498825No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT4830.17254498494250287TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4650.16611473705644686No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT4520.16147066913873975No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG4160.14861017336662774No Hit
GGGGGGAGCAAAAGCAGAAGAAGTGTCATTCCGGGGGAGGGGAGTTTTCG4120.1471812293919486No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG3290.11753064191735703No Hit
GGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGT3260.1164589339363477No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT2970.10609909011992412No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTG2840.10145502220221701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA40150.0294.265261
GGGTTAT40350.0292.806672
GTTATTA40900.0288.147924
GGGGGGA44700.0287.740481
GGTTATT41300.0285.714263
TTATTAG41800.0281.59095
TTAGTAG41850.0280.54968
GGGGAGC46450.0276.89993
TATTAGT42650.0275.978886
GGGAGCG35550.0275.08444
GGAGCGA35600.0274.698065
ATTAGTA43000.0273.732547
CGAAAGC35900.0271.580789
GCGAAAG37100.0262.79658
AGCGAAA37150.0262.44287
GAGCGAA37800.0258.32016
TAGTAGA46100.0254.365519
GGGGGAG52700.0244.62052
GGGAGCA12850.0241.050584
AAACGTT1250.0200.59999295
Produced by FastQC (version 0.11.2)