##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_7098_flu.3510000003ba6c.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	498865
Sequences flagged as poor quality	0
Sequence length	301
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.80865564832169	34.0	34.0	34.0	34.0	34.0
2	33.86697403105048	34.0	34.0	34.0	34.0	34.0
3	33.88780932717268	34.0	34.0	34.0	34.0	34.0
4	33.89870004911148	34.0	34.0	34.0	34.0	34.0
5	33.89169214116043	34.0	34.0	34.0	34.0	34.0
6	37.627179697914265	38.0	38.0	38.0	37.0	38.0
7	37.634380042696925	38.0	38.0	38.0	38.0	38.0
8	37.64874865945697	38.0	38.0	38.0	38.0	38.0
9	37.590696881921964	38.0	38.0	38.0	37.0	38.0
10-14	37.6675585579265	38.0	38.0	38.0	38.0	38.0
15-19	37.62429595181061	38.0	38.0	38.0	37.2	38.0
20-24	37.63419802952703	38.0	38.0	38.0	37.6	38.0
25-29	37.544144808715785	38.0	38.0	38.0	37.0	38.0
30-34	37.50038948412897	38.0	38.0	38.0	37.0	38.0
35-39	37.44269451655258	38.0	38.0	38.0	37.0	38.0
40-44	37.4229424794283	38.0	38.0	38.0	37.0	38.0
45-49	37.408700149339	38.0	38.0	38.0	37.0	38.0
50-54	37.36956852054163	38.0	38.0	38.0	37.0	38.0
55-59	37.331460816052434	38.0	38.0	38.0	37.0	38.0
60-64	37.16223507361711	38.0	38.0	38.0	36.6	38.0
65-69	37.10109628857507	38.0	38.0	38.0	36.8	38.0
70-74	37.16697543423572	38.0	38.0	38.0	37.0	38.0
75-79	37.071251741453096	38.0	38.0	38.0	36.8	38.0
80-84	36.95363875998517	38.0	38.0	38.0	36.0	38.0
85-89	37.0129638278893	38.0	38.0	38.0	36.2	38.0
90-94	36.88469666142143	38.0	38.0	38.0	35.8	38.0
95-99	36.9021266274443	38.0	38.0	38.0	36.0	38.0
100-104	36.89281388752469	38.0	38.0	38.0	36.0	38.0
105-109	36.761317791386446	38.0	38.0	38.0	35.8	38.0
110-114	36.6647786475299	38.0	38.0	38.0	35.0	38.0
115-119	36.74097681737545	38.0	38.0	38.0	35.0	38.0
120-124	36.60535976667034	38.0	37.8	38.0	34.8	38.0
125-129	36.542489851963964	38.0	37.0	38.0	34.4	38.0
130-134	36.461732131939506	38.0	37.0	38.0	34.0	38.0
135-139	36.29201447285338	38.0	37.0	38.0	34.0	38.0
140-144	36.19806039710143	38.0	37.0	38.0	33.8	38.0
145-149	35.757403305503495	38.0	37.0	38.0	30.4	38.0
150-154	35.48393453138625	38.0	36.6	38.0	29.0	38.0
155-159	35.51900053120583	38.0	36.4	38.0	29.6	38.0
160-164	35.478155412787025	38.0	36.0	38.0	29.4	38.0
165-169	35.23506559890952	38.0	36.0	38.0	28.2	38.0
170-174	35.13790905355156	38.0	35.4	38.0	28.0	38.0
175-179	34.80352480129895	38.0	35.2	38.0	26.4	38.0
180-184	34.402382608521336	37.6	35.0	38.0	24.0	38.0
185-189	33.72400428973771	37.0	33.8	37.8	22.8	38.0
190-194	32.73640443807443	36.8	31.6	37.0	21.0	38.0
195-199	32.471388852695625	36.8	30.6	37.2	20.4	37.8
200-204	32.25698214947932	37.0	30.0	37.0	20.0	37.4
205-209	31.329806661120745	35.6	28.4	37.0	18.0	37.2
210-214	30.529856373968908	35.0	26.6	37.0	15.4	37.0
215-219	29.674282621550923	34.2	24.8	37.0	12.8	37.0
220-224	28.296948473033787	32.4	22.0	36.2	9.4	37.0
225-229	27.322426307718523	31.0	20.4	36.0	9.0	37.0
230-234	26.87479257915468	30.8	20.0	36.0	9.0	37.0
235-239	25.89173984945827	28.6	18.6	35.6	8.8	37.0
240-244	24.873275134555442	27.4	16.4	34.0	8.0	37.0
245-249	24.02258807493009	25.6	15.0	34.0	8.0	36.4
250-254	23.028565644011913	24.4	13.2	32.8	8.0	36.0
255-259	22.472072003447824	23.2	12.8	32.0	8.0	36.0
260-264	21.473670030970304	21.8	11.6	30.8	8.0	36.0
265-269	20.60400388882764	20.4	9.0	29.6	8.0	35.8
270-274	19.709525823619614	19.2	9.0	28.2	7.2	34.2
275-279	18.445504094294048	17.6	8.6	26.0	7.0	33.4
280-284	18.036568610746393	17.0	8.2	25.4	7.0	32.4
285-289	16.91268699948884	15.0	8.0	23.4	7.0	30.4
290-294	16.531256351918856	14.4	8.0	22.8	7.0	30.0
295-299	15.649085023002215	13.0	8.0	21.2	7.0	29.0
300-301	13.947537911058102	12.0	8.0	18.5	7.0	25.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.09602672087032005
1101	2	0.05347875915137479
1101	3	0.011014673917188134
1101	4	0.04332662973638435
1101	5	0.05922566667152296
1101	6	0.1265695409720493
1101	7	0.07491300900758802
1101	8	0.07591700841981464
1101	9	0.07618701219480783
1101	10-14	0.0637104992795372
1101	15-19	0.053205079565934454
1101	20-24	0.08963439940079354
1101	25-29	0.04352151635696799
1101	30-34	0.0759976921074994
1101	35-39	0.11208178391697743
1101	40-44	0.07908886218665145
1101	45-49	0.07272758046184435
1101	50-54	0.07056444360269865
1101	55-59	0.1293305638813962
1101	60-64	0.2863533878435405
1101	65-69	0.1770254103146982
1101	70-74	0.16301868264333308
1101	75-79	0.16751873984318166
1101	80-84	0.14482888171413322
1101	85-89	0.15031758631386793
1101	90-94	0.31505527011420753
1101	95-99	0.1480388118070337
1101	100-104	0.1264604543191865
1101	105-109	0.18525001350625558
1101	110-114	0.20423389531703862
1101	115-119	0.08149202294038105
1101	120-124	0.2197043012785258
1101	125-129	0.08130330331286473
1101	130-134	0.19021181192132275
1101	135-139	0.16791348463845424
1101	140-144	0.26202211560608646
1101	145-149	0.5012517190518437
1101	150-154	0.727774187559433
1101	155-159	0.347217156803481
1101	160-164	0.3432110838896989
1101	165-169	0.32580580288914973
1101	170-174	0.4413494561105509
1101	175-179	0.4089626620683049
1101	180-184	0.3067661943310682
1101	185-189	0.321004397125634
1101	190-194	0.49663722324063286
1101	195-199	0.006236054841174621
1101	200-204	-0.04311950896832428
1101	205-209	0.0799376301068726
1101	210-214	-0.0475851789074504
1101	215-219	-0.0699147098834274
1101	220-224	0.2741437142292682
1101	225-229	0.8398051407974982
1101	230-234	0.3061237743153242
1101	235-239	0.422438766064289
1101	240-244	0.6482504440015298
1101	245-249	0.6960916975931752
1101	250-254	0.3043211376657382
1101	255-259	-0.18037202538595665
1101	260-264	0.6178026181427647
1101	265-269	-0.006046936780194301
1101	270-274	0.22504363802353566
1101	275-279	0.41636958490111553
1101	280-284	0.07431822005104394
1101	285-289	0.37130924333416004
1101	290-294	0.3607186040130763
1101	295-299	0.44015494995183246
1101	300-301	0.46597998071865376
1102	1	-0.06487477721793056
1102	2	0.01335278388206973
1102	3	5.189692765412701E-4
1102	4	-0.03426285006107577
1102	5	-0.0066115246747457945
1102	6	0.09295135353630002
1102	7	0.11155747927706727
1102	8	0.007833566867113007
1102	9	0.10800381178187735
1102	10-14	0.06977427791603219
1102	15-19	0.006393005879665736
1102	20-24	0.023724567516104855
1102	25-29	-0.002632161039826997
1102	30-34	0.022165202895415348
1102	35-39	0.008303196178232497
1102	40-44	-0.017354493733101606
1102	45-49	0.05188254917827351
1102	50-54	0.07806019151076526
1102	55-59	0.013514488262117652
1102	60-64	0.13952492128407812
1102	65-69	0.1787033336240782
1102	70-74	0.14061297670466644
1102	75-79	-0.003877219953452027
1102	80-84	0.11765254497892386
1102	85-89	0.1582135884638376
1102	90-94	0.29383253240688134
1102	95-99	0.13683230243476174
1102	100-104	0.16204907729956375
1102	105-109	0.16130886209278117
1102	110-114	0.3126745186849931
1102	115-119	0.18136471917799923
1102	120-124	0.27352033667737885
1102	125-129	0.17584082013992486
1102	130-134	0.25216602522490916
1102	135-139	0.149841225584737
1102	140-144	0.3153841476398327
1102	145-149	0.45829093458278436
1102	150-154	0.9126801905808364
1102	155-159	0.49661412454852183
1102	160-164	0.50112759236616
1102	165-169	0.6054722414369067
1102	170-174	0.5112419639581063
1102	175-179	0.5034909160079266
1102	180-184	0.7254498039564297
1102	185-189	0.8461822049536281
1102	190-194	0.9780098612805119
1102	195-199	0.9337035021904967
1102	200-204	0.5208200701918813
1102	205-209	0.3787922618857671
1102	210-214	0.14843616717292463
1102	215-219	0.5512565944225258
1102	220-224	1.3191268551647113
1102	225-229	1.8384392305390307
1102	230-234	1.300966417967775
1102	235-239	1.494822381235192
1102	240-244	1.698698201011613
1102	245-249	1.9451973109639127
1102	250-254	1.3048983618099435
1102	255-259	1.1024119841889402
1102	260-264	1.7038543550255731
1102	265-269	1.372248655365123
1102	270-274	1.4789496209347277
1102	275-279	1.6422481423526492
1102	280-284	0.8452397006260952
1102	285-289	1.3015952898301997
1102	290-294	0.9721559542909901
1102	295-299	1.1256957654637532
1102	300-301	0.687880981171439
1103	1	0.011657814795505317
1103	2	-0.04031472256104962
1103	3	0.002942158689563712
1103	4	-0.030770538359227828
1103	5	0.029186118159643115
1103	6	0.024873862440827565
1103	7	0.056362158858917155
1103	8	0.06115300045237859
1103	9	0.09535793682169214
1103	10-14	0.06540987285420385
1103	15-19	0.015217492602509708
1103	20-24	0.06208092230716744
1103	25-29	0.05634504916491068
1103	30-34	0.0735287102651796
1103	35-39	-0.39708515699119573
1103	40-44	-0.2712221565483759
1103	45-49	-0.16792551846716464
1103	50-54	0.05283608703100384
1103	55-59	-0.3258866642586682
1103	60-64	0.11297779245079909
1103	65-69	0.1759909136200264
1103	70-74	0.10383574912524551
1103	75-79	0.2093768848158959
1103	80-84	0.1830551453901208
1103	85-89	0.16149682478851446
1103	90-94	0.33109972610565563
1103	95-99	0.27509796136969555
1103	100-104	0.22959084893827253
1103	105-109	0.15821095574232658
1103	110-114	0.29908531415598816
1103	115-119	0.055789993553240436
1103	120-124	0.3186195750870269
1103	125-129	0.2141174830420809
1103	130-134	0.359685441435488
1103	135-139	0.34451831706245173
1103	140-144	0.3824753357787145
1103	145-149	0.6733699604421091
1103	150-154	0.7837834251680462
1103	155-159	-0.08112994634019088
1103	160-164	-0.5951110199756613
1103	165-169	0.35423985502391275
1103	170-174	0.7303311829660117
1103	175-179	0.49970161302237415
1103	180-184	0.5389783129188643
1103	185-189	0.6280670107915185
1103	190-194	0.4612176914719228
1103	195-199	0.5174827278586989
1103	200-204	0.15875561011022654
1103	205-209	0.6242802299163479
1103	210-214	0.4874042267101757
1103	215-219	0.35730147902566856
1103	220-224	0.9267103260476368
1103	225-229	1.7469993903861365
1103	230-234	0.8237069849616638
1103	235-239	1.2302222596123968
1103	240-244	1.3626912511375835
1103	245-249	1.2085718223008932
1103	250-254	1.365383347186249
1103	255-259	0.5837372093359043
1103	260-264	1.6089819720875127
1103	265-269	0.8853448821626806
1103	270-274	0.9073463429108095
1103	275-279	1.8513304694021429
1103	280-284	0.6495822135847114
1103	285-289	0.7304940243739715
1103	290-294	1.0574777045009363
1103	295-299	1.0443310418660374
1103	300-301	-0.6479760251703226
2116	1	0.0409162367442093
2116	2	0.05086560622289937
2116	3	0.059744190563186805
2116	4	0.043030014265738714
2116	5	0.036063228880010456
2116	6	-0.2781220347374358
2116	7	-0.15232968294520788
2116	8	0.0456728028470863
2116	9	0.05235087550265405
2116	10-14	0.09286550934977811
2116	15-19	0.11695720066022375
2116	20-24	-0.17013543874114134
2116	25-29	-0.17627042991628628
2116	30-34	0.059094369368146715
2116	35-39	-0.13625208880620931
2116	40-44	-0.11363037335598847
2116	45-49	0.09614034996343435
2116	50-54	0.2075217327369927
2116	55-59	0.12482142137029939
2116	60-64	0.36195979027379366
2116	65-69	0.2241944359064405
2116	70-74	-0.7177455397748105
2116	75-79	0.23546882151521942
2116	80-84	-0.04541687448205067
2116	85-89	0.15612854769453577
2116	90-94	0.24073929182058862
2116	95-99	0.25735635540803514
2116	100-104	0.23595831663204336
2116	105-109	0.26198555173998983
2116	110-114	0.43268066987342735
2116	115-119	0.34697132710984846
2116	120-124	-0.0831877876638174
2116	125-129	0.08036259175290184
2116	130-134	0.27564223621067185
2116	135-139	0.506917714198849
2116	140-144	0.4279976780154513
2116	145-149	0.7113611965915894
2116	150-154	1.0477119746670596
2116	155-159	-0.26458837387096423
2116	160-164	-0.42312452858600835
2116	165-169	0.6736376612702415
2116	170-174	0.8599495368134598
2116	175-179	0.6412776547762604
2116	180-184	0.5569095976323766
2116	185-189	0.936282032142941
2116	190-194	0.9235915227259355
2116	195-199	0.758655409206213
2116	200-204	0.6597697606586692
2116	205-209	0.6240327209152632
2116	210-214	0.6052977766278325
2116	215-219	1.016551695330893
2116	220-224	1.2465116043417552
2116	225-229	0.8007825269514939
2116	230-234	0.8351055726978593
2116	235-239	0.6860671896969635
2116	240-244	1.3641890322646013
2116	245-249	0.877653962466681
2116	250-254	1.0245012684232329
2116	255-259	0.7821839424292065
2116	260-264	0.1546060196467529
2116	265-269	0.6252713104749361
2116	270-274	0.9290351424865726
2116	275-279	1.3406458284688192
2116	280-284	-0.07466015991161612
2116	285-289	-1.041798811038685
2116	290-294	-0.03620082406531111
2116	295-299	0.47702478157409445
2116	300-301	1.04026394803574
1117	1	-0.037278954673581666
1117	2	0.03337912337614313
1117	3	0.014354635805460703
1117	4	0.021193973681327805
1117	5	0.0019223524928335678
1117	6	-0.4970188287294306
1117	7	-0.18892442049610025
1117	8	-0.10611358171914276
1117	9	-0.04894568238686503
1117	10-14	0.09531157683955627
1117	15-19	0.10398921489514379
1117	20-24	0.06650846772330965
1117	25-29	0.13678724040558166
1117	30-34	0.0492403001820918
1117	35-39	-0.12589349384516169
1117	40-44	-0.06752070604844818
1117	45-49	0.1565955349056054
1117	50-54	0.0981716735681104
1117	55-59	0.1775663705251631
1117	60-64	0.2120939732703988
1117	65-69	0.22784783518036278
1117	70-74	-0.1396009499312214
1117	75-79	0.17019817759156552
1117	80-84	0.06259943492680264
1117	85-89	0.05481044258882406
1117	90-94	0.25296481552968686
1117	95-99	0.21805022161472465
1117	100-104	0.19220738182097108
1117	105-109	0.21107956579725595
1117	110-114	0.29551567607684603
1117	115-119	0.2812658420252845
1117	120-124	0.4383767923803603
1117	125-129	0.3829703206705872
1117	130-134	0.2428996366192564
1117	135-139	0.3281532980510491
1117	140-144	0.29639450209332097
1117	145-149	0.8154530473312818
1117	150-154	0.8508477823008462
1117	155-159	-0.04839483860888549
1117	160-164	-0.5297940223696855
1117	165-169	0.37755713428034454
1117	170-174	0.5432961174822708
1117	175-179	0.6273557462430475
1117	180-184	0.6888417124400661
1117	185-189	0.8958946407027994
1117	190-194	1.1088008487737113
1117	195-199	0.8081775595725418
1117	200-204	0.7414966325014376
1117	205-209	1.1892299553071375
1117	210-214	0.7211673586634184
1117	215-219	0.13630218315184806
1117	220-224	0.8013927651407187
1117	225-229	0.40571844404055213
1117	230-234	1.1400173637815492
1117	235-239	0.6094888417839179
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2108	175-179	-0.45022829895237493
2108	180-184	-0.34108479952092097
2108	185-189	-0.501385904006689
2108	190-194	-0.14745063604842557
2108	195-199	-1.1358599062552308
2108	200-204	-0.671544340064159
2108	205-209	-0.3304003879212196
2108	210-214	0.09409613835275721
2108	215-219	-0.4602165412367505
2108	220-224	-0.009888245618199676
2108	225-229	0.3051797590956582
2108	230-234	0.06856664282581093
2108	235-239	-0.1416541728739027
2108	240-244	-0.0032718779107483442
2108	245-249	0.25787526026800833
2108	250-254	-0.12822834522365056
2108	255-259	-0.4120688029544155
2108	260-264	0.28646677527635234
2108	265-269	-0.5721910753194663
2108	270-274	-0.5542195099375569
2108	275-279	0.10986227475008548
2108	280-284	-0.42042609068367653
2108	285-289	0.1043488657624394
2108	290-294	-0.3022784821790161
2108	295-299	0.2751757520470708
2108	300-301	-0.14269451047176673
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	18.0
14	102.0
15	248.0
16	441.0
17	612.0
18	1398.0
19	1291.0
20	1104.0
21	1379.0
22	2175.0
23	3325.0
24	5222.0
25	7882.0
26	11399.0
27	15890.0
28	22399.0
29	32378.0
30	41011.0
31	51053.0
32	68278.0
33	85846.0
34	88604.0
35	50178.0
36	6620.0
37	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.60466258406583	23.571306866587154	18.431439367363918	30.392591181983104
2	25.83825283393303	27.005101580587937	18.835957623806042	28.320687961672995
3	38.1720505547593	26.806651097992447	21.533481001874254	13.487817345373998
4	39.43491726218516	25.843464664789074	21.103705411283613	13.617912661742155
5	38.20272017479679	25.882954306275245	21.76039609914506	14.153929419782907
6	23.633247471760896	26.314533992162207	35.09266033896946	14.959558197107434
7	17.37023042306035	32.57213875497379	35.67137401902318	14.386256802942679
8	23.178014091988814	36.07208362983974	24.477564070439897	16.27233820773155
9	14.89461076643982	22.31966564100508	39.272348230483196	23.513375362071905
10-14	22.18135166828701	33.51403686368056	28.29258416605695	16.012027301975486
15-19	21.70166277449811	32.90549547472763	24.373728363384885	21.019113387389375
20-24	21.10554959758652	30.514948934080362	28.58156014152125	19.797941326811863
25-29	17.494111633407837	28.60822066090024	34.09816282962325	19.799504876068678
30-34	17.32965832439638	30.43316328064707	31.968207831778134	20.268970563178414
35-39	19.175327994547622	31.3394605755064	30.940154149920318	18.54505728002566
40-44	18.9066380684153	29.043288264360097	33.11533180319325	18.934741864031352
45-49	20.044701472342215	29.73277339560803	31.455804676615916	18.766720455433834
50-54	18.88683311116234	31.935934571477254	31.239834424142803	17.937397893217604
55-59	20.075050364327023	31.841399977949948	27.866136129012858	20.21741352871017
60-64	18.632616038407185	31.0600663506159	29.51636214206248	20.790955468914436
65-69	18.374149318954025	31.759053050424463	29.758672185861908	20.108125444759605
70-74	19.54753289968228	31.805197799003736	27.805358163030082	20.841911138283905
75-79	19.859841840979023	33.64216772072604	28.20566686378078	18.292323574514146
80-84	20.308420113658006	31.866055946999687	26.950096719553386	20.875427219788918
85-89	20.075731911438965	31.913002515710666	26.896976135828332	21.11428943702204
90-94	19.232297314904834	32.00256582442144	29.25845669670151	19.506680163972216
95-99	19.859841840979023	30.078077235324187	28.769627053411245	21.29245387028555
100-104	19.023583534623594	28.53577621200124	30.891323303899853	21.54931694947531
105-109	19.73367544325619	29.027773044811724	31.716035400358816	19.52251611157327
110-114	18.808134465236087	29.135056578433044	30.71784951840678	21.33895943792409
115-119	18.23078387940625	30.60242751044872	30.31697954356389	20.84980906658114
120-124	21.248313672035522	29.354715203511972	28.515690617702184	20.881280506750326
125-129	20.70656390005312	30.16415262646207	29.705992603209285	19.423290870275526
130-134	20.01992523027272	29.4558648131258	30.511942108586492	20.012267848014993
135-139	19.3184127970493	27.612159602297215	31.578322792739517	21.491104807913967
140-144	19.51221272288094	29.557535605825226	30.245898188888777	20.68435348240506
145-149	19.261202930652583	30.916420274022027	28.66919908191595	21.15317771340944
150-154	19.055736521904723	28.858268268970562	29.414130075270865	22.67186513385385
155-159	19.16310023753921	30.874805809186856	29.839174927084482	20.12291902618945
160-164	20.178725707355696	29.231154721217163	28.351598127749995	22.238521443677147
165-169	19.812935363274633	29.2531646838323	28.955889870004913	21.978010082888154
170-174	18.41243623024265	30.823729866797635	31.436841630501238	19.326992272458483
175-179	19.37261583795215	30.377116053441313	28.9418179266936	21.308450181912942
180-184	21.299149068385233	28.89643490723943	28.67236627143616	21.13204975293917
185-189	18.611447986930333	30.18407785673479	28.713259098152804	22.491215058182075
190-194	19.95136960901246	29.67861044571177	28.231445381014904	22.13857456426087
195-199	19.362031812213726	30.975995509807262	29.69428602928648	19.96768664869253
200-204	20.724324215970253	28.556463171399077	29.308851091978795	21.41036152065188
205-209	20.645665661050586	29.081013901556535	29.635031521553927	20.638288915838952
210-214	19.91793370952061	28.547522876930632	29.46528619967326	22.0692572138755
215-219	19.167630521283314	30.071502310244252	28.238220761127756	22.522646407344673
220-224	19.4864742966534	29.711805799164104	29.749611618373706	21.052108285808785
225-229	18.086977438786043	31.399356539344314	28.90886311928077	21.604802902588876
230-234	17.067222595291312	31.385565233079088	27.45183566696401	24.09537650466559
235-239	18.213143836508873	30.46623836107965	27.188958936786506	24.13165886562497
240-244	16.932637086185643	31.228408487266098	28.582241688633196	23.256712737915066
245-249	19.20002405460395	30.876890541529274	28.0799013761238	21.843184027742975
250-254	19.650246058552913	31.42268950517675	26.47393583434396	22.453128601926373
255-259	18.433323644673408	31.364958455694424	26.229300512162606	23.972417387469555
260-264	17.0131799184148	32.576147855632286	27.441893097330944	22.96877912862197
265-269	17.50016537540216	32.59158289316749	26.57893418058994	23.329317550840408
270-274	17.012738917342368	32.243873593056236	27.345514317500726	23.39787317210067
275-279	17.35615847974903	32.63937137301674	25.11372816292985	24.89074198430437
280-284	17.90444308580478	31.401802090745996	26.833311617371436	23.860443206077797
285-289	18.129954997845108	32.97172581760597	25.659968127649762	23.238351056899162
290-294	15.828971765908612	34.580096819780906	24.83734076353322	24.753590650777266
295-299	16.94727030358915	32.136189149369066	25.483287061629902	25.433253485411882
300-301	12.535455483948562	35.75837150331252	32.44695458691229	19.259218425826628
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.25
25	0.0
26	0.25
27	0.75
28	3.25
29	8.5
30	22.75
31	84.75
32	546.25
33	1811.75
34	4566.25
35	7466.0
36	10311.5
37	10908.25
38	12170.5
39	12967.5
40	15728.0
41	18148.0
42	16441.25
43	14544.5
44	13498.75
45	10957.25
46	7142.75
47	4505.5
48	2638.25
49	1159.0
50	469.5
51	206.25
52	85.25
53	48.75
54	30.75
55	20.0
56	16.75
57	16.25
58	13.5
59	12.0
60	8.5
61	5.75
62	3.5
63	2.75
64	2.75
65	1.5
66	1.25
67	0.75
68	0.5
69	0.25
70	0.25
71	0.0
72	0.75
73	0.25
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	498865.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.835229052363552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	38.01795135013128	3.3589730828002393
2	4.226240024894925	0.7467959730042659
3	3.938017084304691	1.0437984885585843
4	2.6371153722772003	0.9319807340631215
5	2.612611311057284	1.1541509678993471
6	2.767143969869433	1.4669010476797812
7	2.9174962704190883	1.8043723465998325
8	2.9673448291601416	2.0973736994381156
9	3.1370054057073937	2.4944545168134726
>10	35.84334402783358	60.34573954967817
>50	0.6903842272871847	3.825739860263914
>100	0.15303349671470312	2.4918385213293606
>500	0.02026375705027705	1.34594462820992
>1k	0.06079123684084016	10.494823038853921
>5k	0.01125763645200544	6.397113544807924
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA	7370	1.4773535926553276	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT	7329	1.4691349363054134	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG	6286	1.2600603369649104	No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT	6037	1.2101470337666504	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA	5136	1.0295370491014604	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA	4377	0.8773916791115832	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT	3751	0.7519068285006967	No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT	2504	0.5019394024435468	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG	2499	0.5009371272789231	No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	2480	0.4971284816533531	No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	2378	0.4766820682950297	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG	2289	0.45884157036472795	No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	2205	0.4420033475990498	No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	2156	0.43218105098573767	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT	2008	0.40251370611287624	No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAACATAAATCCGTATTTTCT	1966	0.3940945947300372	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT	1932	0.3872791236105961	No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA	1822	0.3652290699888748	No Hit
GGGGGGAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA	1811	0.3630240646267026	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT	1770	0.3548054082767883	TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT	1756	0.35199903781584196	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTGACAAGA	1750	0.3507963076182935	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA	1557	0.3121084862638189	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1514	0.3034889198480551	No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT	1513	0.30328846481513033	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG	1470	0.29466889839936655	No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAACATAAATCCGTATTTTCT	1447	0.2900584326420976	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	1326	0.2658033736582041	No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	1199	0.24034558447676227	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA	1130	0.22651418720495525	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT	1094	0.21929780601966462	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC	1053	0.21107914966975033	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA	953	0.19103364637727643	No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	881	0.17660088400669519	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA	864	0.17319314844697464	No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAACATAAATCCGTATTTTCT	862	0.17279223838112515	No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA	824	0.16517494712998507	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG	789	0.1581590209776192	No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG	548	0.10984935804275707	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG	531	0.1064416224830365	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA	514	0.10303388692331591	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.0045503292473915E-4	0.0	0.0
2	2.0045503292473915E-4	0.0	0.0
3	2.0045503292473915E-4	0.0	0.0
4	2.0045503292473915E-4	0.0	0.0
5	4.009100658494783E-4	0.0	0.0
6	6.013650987742175E-4	0.0	0.0
7	8.018201316989566E-4	0.0	0.0
8	0.0010022751646236957	0.0	0.0
9	0.0010022751646236957	0.0	0.0
10-14	0.0022050053621721307	0.0	0.0
15-19	0.0029667344872861398	0.0	0.0
20-24	0.0033275535465506698	0.0	0.0
25-29	0.0036883726058152	0.0	0.0
30-34	0.008419111382839045	0.0	0.0
35-39	0.00942138654746274	0.0	0.0
40-44	0.012307939021578984	0.0	0.0
45-49	0.017680133903961993	0.0	0.0
50-54	0.023172601806099848	0.0	0.0
55-59	0.032593988353562586	0.0	0.0
60-64	0.051156124402393435	0.0	0.0
65-69	0.07200344782656631	0.0	0.0
70-74	0.10247261283112666	0.0	0.0
75-79	0.14565062692311545	0.0	0.0
80-84	0.1977288444769627	0.0	0.0
85-89	0.268208834053301	0.0	0.0
90-94	0.3674340753510469	0.0	0.0
95-99	0.49444238421216163	0.0	0.0
100-104	0.651679312038327	0.0	0.0
105-109	0.8526154370420855	0.0	0.0
110-114	1.1012999508885168	0.0	0.0
115-119	1.3740791596925022	0.0	0.0
120-124	1.7002996802742225	0.0	0.0
125-129	2.0891022621350466	0.0	0.0
130-134	2.5350746193860063	0.0	0.0
135-139	3.0455534062321465	0.0	0.0
140-144	3.8905114610165077	0.0	0.0
145-149	4.869413568801178	0.0	0.0
150-154	5.749491345353954	0.0	0.0
155-159	7.40428773315426	0.0	0.0
160-164	8.221422629368666	0.0	0.0
165-169	9.106351417718221	0.0	0.0
170-174	9.977408717789382	0.0	0.0
175-179	10.879817185009973	0.0	0.0
180-184	11.821915748749662	0.0	0.0
185-189	12.84471750874485	0.0	0.0
190-194	13.888166137131288	0.0	0.0
195-199	14.987822356749822	0.0	0.0
200-204	16.086596574223485	0.0	0.0
205-209	17.18801679813176	0.0	0.0
210-214	18.347729345614546	0.0	0.0
215-219	19.417918675393146	0.0	0.0
220-224	20.542832229160194	0.0	0.0
225-229	21.624567768835256	0.0	0.0
230-234	22.849027291952734	0.0	0.0
235-239	23.970212382107384	0.0	0.0
240-244	25.09873412646708	0.0	0.0
245-249	26.206127910356507	0.0	0.0
250-254	27.420604772834338	0.0	0.0
255-259	28.51721407595241	0.0	0.0
260-264	29.633427881290526	0.0	0.0
265-269	30.626722660439196	0.0	0.0
270-274	31.59965120824271	0.0	0.0
275-279	32.536978942198786	0.0	0.0
280-284	33.42667856033195	0.0	0.0
285-289	34.272037525182164	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTT	6215	0.0	292.3894	1
CGGGTTA	6290	0.0	289.1375	2
GTTATTA	7005	0.0	258.99356	5
TTATTAG	7105	0.0	255.14076	6
GGGGGGA	4455	0.0	254.60718	1
GGGTTAT	7155	0.0	253.97626	3
TATTAGT	7455	0.0	243.95372	7
ATTAGTA	7520	0.0	242.43353	8
GAGCAGA	4865	0.0	232.84685	6
GGGGAGC	4875	0.0	232.36923	3
CAGAAGC	4920	0.0	229.9441	9
GGAGCAG	4985	0.0	226.94583	5
GGGAGCA	5005	0.0	226.33366	4
TTAGTAG	8290	0.0	220.09349	9
GGGGGAG	5210	0.0	217.42802	2
GGTTATT	8385	0.0	216.89624	4
CGGGCTA	15	0.0028492154	196.66666	2
GCAGAAG	6040	0.0	187.54967	8
CCGGGTA	25	5.5546247E-5	177.0	1
AGCAGAA	8425	0.0	134.63205	7
>>END_MODULE