##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_6957_flu.3510000003b994.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	281933
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.80383637247147	34.0	34.0	34.0	34.0	34.0
2	33.88757257930076	34.0	34.0	34.0	34.0	34.0
3	33.890392398193896	34.0	34.0	34.0	34.0	34.0
4	33.89648604455669	34.0	34.0	34.0	34.0	34.0
5	33.88659362330767	34.0	34.0	34.0	34.0	34.0
6	37.65123273969348	38.0	38.0	38.0	38.0	38.0
7	37.662398513121914	38.0	38.0	38.0	38.0	38.0
8	37.67070190435316	38.0	38.0	38.0	38.0	38.0
9	37.58447929117911	38.0	38.0	38.0	37.0	38.0
10-14	37.665432567312095	38.0	38.0	38.0	38.0	38.0
15-19	37.63530838887253	38.0	38.0	38.0	37.6	38.0
20-24	37.63118329532194	38.0	38.0	38.0	37.2	38.0
25-29	37.55217516218392	38.0	38.0	38.0	37.0	38.0
30-34	37.502777610283296	38.0	38.0	38.0	37.0	38.0
35-39	37.432104081466164	38.0	38.0	38.0	37.0	38.0
40-44	37.43477067246474	38.0	38.0	38.0	37.0	38.0
45-49	37.414507702184565	38.0	38.0	38.0	37.0	38.0
50-54	37.37974128605023	38.0	38.0	38.0	37.0	38.0
55-59	37.330939620406276	38.0	38.0	38.0	37.0	38.0
60-64	37.16282166330298	38.0	38.0	38.0	36.6	38.0
65-69	37.09699538542846	38.0	38.0	38.0	36.4	38.0
70-74	37.17699311538557	38.0	38.0	38.0	37.0	38.0
75-79	37.10659979498675	38.0	38.0	38.0	36.8	38.0
80-84	37.008436756250596	38.0	38.0	38.0	36.4	38.0
85-89	37.04381892151682	38.0	38.0	38.0	36.0	38.0
90-94	36.89535244189223	38.0	38.0	38.0	35.8	38.0
95-99	36.90280669520773	38.0	38.0	38.0	36.0	38.0
100-104	36.88407103815446	38.0	38.0	38.0	36.0	38.0
105-109	36.745433844211206	38.0	38.0	38.0	35.2	38.0
110-114	36.656928419163414	38.0	38.0	38.0	34.8	38.0
115-119	36.748859480798636	38.0	38.0	38.0	35.2	38.0
120-124	36.664298255259226	38.0	37.8	38.0	34.8	38.0
125-129	36.572641726935124	38.0	37.4	38.0	34.4	38.0
130-134	36.46349948391993	38.0	37.0	38.0	34.0	38.0
135-139	36.22912535957125	38.0	37.0	38.0	33.8	38.0
140-144	36.18652942365739	38.0	37.0	38.0	34.0	38.0
145-149	35.7777365544296	38.0	37.0	38.0	30.6	38.0
150-154	35.39271458112388	38.0	36.4	38.0	28.6	38.0
155-159	35.506847371538626	38.0	36.2	38.0	29.6	38.0
160-164	35.464848031269845	38.0	36.0	38.0	29.4	38.0
165-169	35.270831013042105	38.0	36.0	38.0	28.4	38.0
170-174	35.13388641982315	38.0	35.6	38.0	28.0	38.0
175-179	34.77033905218616	38.0	35.2	38.0	25.8	38.0
180-184	34.500318870086154	37.6	35.0	38.0	24.4	38.0
185-189	33.728898709977194	37.0	33.8	37.8	22.8	38.0
190-194	32.72272490272512	36.8	31.2	37.0	21.2	38.0
195-199	32.48534013400347	36.8	30.6	37.2	20.6	37.8
200-204	32.256460222818895	37.0	30.2	37.0	20.0	37.4
205-209	31.180781249445793	35.4	28.2	37.0	18.0	37.2
210-214	30.42475765518758	34.4	26.4	37.0	15.8	37.0
215-219	29.519854007867117	33.2	24.6	37.0	13.8	37.0
220-224	27.9819552872491	31.6	21.8	36.2	9.4	37.0
225-229	26.955163815516453	30.0	20.0	36.2	9.0	37.0
230-234	26.49816871384336	29.4	19.8	35.8	9.0	37.0
235-239	25.407079696239883	27.6	18.0	34.6	8.8	37.0
240-244	24.266665484352664	26.0	16.2	33.4	8.0	37.0
245-249	23.35236669705214	24.6	15.0	32.8	8.0	36.2
250-254	22.417617660933626	23.0	13.2	31.6	8.0	36.0
255-259	21.682225918923997	21.8	12.4	30.8	8.0	36.0
260-264	20.378361525610696	19.8	10.6	29.2	7.4	35.0
265-269	19.536814775141615	18.8	9.0	27.8	7.0	34.0
270-274	18.5341978413311	17.6	8.2	26.2	7.0	33.0
275-279	17.262006930724674	15.8	8.0	24.2	7.0	31.0
280-284	16.929163311850687	15.2	8.0	23.8	7.0	30.4
285-289	15.812244043797639	13.4	8.0	21.4	7.0	29.0
290-294	15.143656826267232	12.8	8.0	20.4	7.0	27.6
295-299	14.370931391500818	12.2	8.0	19.0	7.0	26.2
300-301	12.74044010456385	10.0	8.0	16.0	7.0	22.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.009871342167713237
1101	2	0.04606407834259585
1101	3	0.027600856133702223
1101	4	0.034158420999233385
1101	5	0.04270280405064142
1101	6	0.07007978440277185
1101	7	0.07325976603452489
1101	8	0.10025500630706574
1101	9	0.06733110544984555
1101	10-14	0.03435905492944613
1101	15-19	0.07431805469447283
1101	20-24	0.05096735548099218
1101	25-29	0.09016361756219027
1101	30-34	0.11686007979576374
1101	35-39	0.1451574781749656
1101	40-44	0.10927726324649001
1101	45-49	0.1622941974951715
1101	50-54	0.09886289309476837
1101	55-59	0.17835301501988
1101	60-64	0.20286692979252052
1101	65-69	0.2840566359572776
1101	70-74	0.17142789649624746
1101	75-79	0.17090918324363713
1101	80-84	0.3290459478702914
1101	85-89	0.18132302477273754
1101	90-94	0.43937115627660006
1101	95-99	0.2992570640439709
1101	100-104	0.23480041341693436
1101	105-109	0.35837308251898037
1101	110-114	0.3127681192162939
1101	115-119	0.3022139225567031
1101	120-124	0.3449215652091624
1101	125-129	0.43888015262027835
1101	130-134	0.36192160801495277
1101	135-139	0.3385281933215225
1101	140-144	0.38333867315449055
1101	145-149	0.6625124240523448
1101	150-154	0.9256244753277514
1101	155-159	0.6224275864458875
1101	160-164	0.6746637243168934
1101	165-169	0.5203782507341401
1101	170-174	0.6382874592915897
1101	175-179	0.6349065454240872
1101	180-184	0.4267189271737024
1101	185-189	0.4165953970469971
1101	190-194	0.8170865379012682
1101	195-199	0.4483339579527623
1101	200-204	0.10142988999283631
1101	205-209	-0.22849714310175884
1101	210-214	-0.24818165769545786
1101	215-219	-0.22548277790197346
1101	220-224	0.5627402621994158
1101	225-229	0.6617462702685977
1101	230-234	0.45976488197844745
1101	235-239	0.18941979727643599
1101	240-244	0.3326353570744942
1101	245-249	0.574467474874325
1101	250-254	0.6101351004185105
1101	255-259	-0.16451783837474565
1101	260-264	0.13399583260848047
1101	265-269	0.061055812513245655
1101	270-274	0.08275343087080245
1101	275-279	0.2418392832211005
1101	280-284	0.05226615448470895
1101	285-289	0.2741566364355492
1101	290-294	0.13735381321069617
1101	295-299	0.06536899748706837
1101	300-301	-0.2874208228061885
1102	1	0.0454935056182606
1102	2	0.01129083904393724
1102	3	0.00755318577747488
1102	4	-0.007357235121183692
1102	5	0.037788839248342754
1102	6	0.14808285359008977
1102	7	0.09716024600318462
1102	8	-0.021875793314180214
1102	9	0.09554150803579375
1102	10-14	0.11000799214171053
1102	15-19	0.09508731057979958
1102	20-24	0.09574157430751029
1102	25-29	0.12554742923163076
1102	30-34	0.15578651725290626
1102	35-39	0.1285936619343886
1102	40-44	0.14464474945147288
1102	45-49	0.13204964203757186
1102	50-54	0.1717528268133819
1102	55-59	0.14319122966708875
1102	60-64	0.2744051150538809
1102	65-69	0.30734654355515545
1102	70-74	0.3082940603386035
1102	75-79	0.028263804982628926
1102	80-84	0.1743458466524146
1102	85-89	0.21759494170876792
1102	90-94	0.45030268727865774
1102	95-99	0.23927730761990773
1102	100-104	0.28686231957816943
1102	105-109	0.22504954446431924
1102	110-114	0.26327257606809695
1102	115-119	0.23222339132608738
1102	120-124	0.36874368294840565
1102	125-129	0.21140766102789144
1102	130-134	0.2886333329635278
1102	135-139	0.23754213526817125
1102	140-144	0.30537478508187377
1102	145-149	0.6370838806102839
1102	150-154	0.9689783787463071
1102	155-159	0.7090080546602096
1102	160-164	0.8484287029305335
1102	165-169	0.5528234788008817
1102	170-174	0.5834469590793532
1102	175-179	0.8157241246842162
1102	180-184	0.7549619806885772
1102	185-189	1.0219468842968098
1102	190-194	1.1690864072975486
1102	195-199	1.2657695539954688
1102	200-204	0.6911938237767572
1102	205-209	0.41948339694460657
1102	210-214	0.1698514503464672
1102	215-219	0.6005498077250913
1102	220-224	1.448762399529226
1102	225-229	1.8242727991483463
1102	230-234	1.1529408378343966
1102	235-239	1.08998139325384
1102	240-244	1.0825488321128631
1102	245-249	1.6584913753542914
1102	250-254	0.9945312214881525
1102	255-259	0.9563330448328813
1102	260-264	1.4495278794952178
1102	265-269	1.3215514536142372
1102	270-274	1.432869341668468
1102	275-279	1.5161854483694945
1102	280-284	0.9601154377968228
1102	285-289	1.231850827835295
1102	290-294	1.3201402434846372
1102	295-299	0.8510613604347945
1102	300-301	0.657856220325689
1103	1	0.08594145924589469
1103	2	0.014842643893572927
1103	3	0.007173142658565723
1103	4	-0.01677839748484189
1103	5	0.045473169040526784
1103	6	-0.022545851386347238
1103	7	0.04206464917565711
1103	8	0.10915561529610329
1103	9	0.15731094617578378
1103	10-14	0.04160200664017566
1103	15-19	0.05407000263477357
1103	20-24	0.05864261747260713
1103	25-29	0.04801827779333223
1103	30-34	0.06256513680843057
1103	35-39	-0.5971775236935812
1103	40-44	-0.3370048507928871
1103	45-49	-0.1297506968046065
1103	50-54	0.003117394718564981
1103	55-59	-0.2668792026230804
1103	60-64	0.12831867122745422
1103	65-69	0.3351404820449986
1103	70-74	0.15367405018044877
1103	75-79	0.20197902837338688
1103	80-84	0.17965011201486192
1103	85-89	0.23105379582548835
1103	90-94	0.27678494423753364
1103	95-99	0.2836812614890647
1103	100-104	0.2824854304619606
1103	105-109	0.06920522717448563
1103	110-114	0.3132695570339692
1103	115-119	-0.014190884087959432
1103	120-124	0.3292390886888299
1103	125-129	0.2849257592495036
1103	130-134	0.4646721470869153
1103	135-139	0.23857819674280734
1103	140-144	0.416627968386976
1103	145-149	0.7348963240033939
1103	150-154	0.7355090112872276
1103	155-159	-0.0721904663682551
1103	160-164	-0.5940150098421171
1103	165-169	0.36559257241579957
1103	170-174	0.7235876815875031
1103	175-179	0.6984431733688083
1103	180-184	0.7348456235617036
1103	185-189	0.7056604383393008
1103	190-194	0.4822366095728441
1103	195-199	0.5760637499911709
1103	200-204	0.38581426015149134
1103	205-209	0.8747860639948684
1103	210-214	0.5935345299033408
1103	215-219	0.6652420260758483
1103	220-224	1.265732923100778
1103	225-229	2.248448869218354
1103	230-234	1.0165113962136338
1103	235-239	1.3800370500358277
1103	240-244	1.3225613344659308
1103	245-249	0.9909296538655141
1103	250-254	1.4914880350929884
1103	255-259	0.6068514307506874
1103	260-264	1.6634552166716041
1103	265-269	0.6406491531102319
1103	270-274	0.8697100822908155
1103	275-279	1.4595647065395383
1103	280-284	0.5346770562832539
1103	285-289	0.8758964045951245
1103	290-294	0.75632247948786
1103	295-299	0.8946313505252572
1103	300-301	-1.0252002498196155
2116	1	0.08007045217824071
2116	2	0.034052924372304005
2116	3	0.031632794162497646
2116	4	0.043185305877351254
2116	5	0.04775595472977301
2116	6	-0.2840111511444263
2116	7	-0.0899173164720466
2116	8	0.10136617033074913
2116	9	0.09268151896159793
2116	10-14	0.12302147800780006
2116	15-19	0.1072074515440562
2116	20-24	-0.09877945785932951
2116	25-29	-0.22290314223060648
2116	30-34	0.029415704137662146
2116	35-39	-0.17170840432738288
2116	40-44	-0.15203359282602236
2116	45-49	0.08986640992857531
2116	50-54	0.19979785825184138
2116	55-59	0.08436864539381617
2116	60-64	0.3701129891286783
2116	65-69	0.29417881108731336
2116	70-74	-0.8472936299256375
2116	75-79	0.24288509744060605
2116	80-84	-0.18378905485470654
2116	85-89	0.1329810402337941
2116	90-94	0.2961696281615076
2116	95-99	0.2793450047395609
2116	100-104	0.2079726808794078
2116	105-109	0.23521589513968166
2116	110-114	0.3731593547777763
2116	115-119	0.24995746590003876
2116	120-124	-0.1965465470289942
2116	125-129	-0.02581075858713433
2116	130-134	0.11218378981196508
2116	135-139	0.5484143783779558
2116	140-144	0.4975017814506515
2116	145-149	0.671763658238099
2116	150-154	0.9633141007596535
2116	155-159	-0.32223009566596517
2116	160-164	-0.695627982695612
2116	165-169	0.6561804847029578
2116	170-174	0.8008211249158705
2116	175-179	0.6494109398132366
2116	180-184	0.7285311827837688
2116	185-189	0.9442205220529516
2116	190-194	0.8268785385785478
2116	195-199	0.8249391931226882
2116	200-204	0.8491755498390674
2116	205-209	0.8084644053317618
2116	210-214	0.7485536365778138
2116	215-219	1.0144609231208292
2116	220-224	1.2961889449409192
2116	225-229	0.9030381836401276
2116	230-234	0.9986953072367974
2116	235-239	1.1588278643272432
2116	240-244	1.3398621562342399
2116	245-249	0.7437961730663005
2116	250-254	0.9713396022201835
2116	255-259	0.6579012186173898
2116	260-264	0.04332802303024863
2116	265-269	0.7483710814943088
2116	270-274	0.6565162026430045
2116	275-279	1.3760202759667877
2116	280-284	0.19641587590001564
2116	285-289	-0.9678781694546785
2116	290-294	0.25699125626353947
2116	295-299	0.6626619902750814
2116	300-301	1.0731598397650455
1117	1	-0.050705190953152623
1117	2	0.008431869435476358
1117	3	0.03284435021727461
1117	4	0.036744278280011144
1117	5	0.04663093839319288
1117	6	-0.48792345285367134
1117	7	-0.21529570697457956
1117	8	-0.052877631482701304
1117	9	-0.04172172786734052
1117	10-14	0.11464337361617538
1117	15-19	0.10528732947007313
1117	20-24	0.04999079508390736
1117	25-29	0.15187247264292125
1117	30-34	0.18604800217338635
1117	35-39	-0.04442013522771049
1117	40-44	-0.03154769880008246
1117	45-49	0.21667005660633265
1117	50-54	0.12790560549935037
1117	55-59	0.2916164350987742
1117	60-64	0.3164795572460335
1117	65-69	0.28214837736437204
1117	70-74	-0.0707515451308467
1117	75-79	0.18576287863288599
1117	80-84	0.10939020245790942
1117	85-89	0.11274522833227962
1117	90-94	0.3516242795830564
1117	95-99	0.2810168108058235
1117	100-104	0.17087751897948777
1117	105-109	0.2810134204569579
1117	110-114	0.34328231182228564
1117	115-119	0.3902012606490217
1117	120-124	0.47038786292907986
1117	125-129	0.36353390615093417
1117	130-134	0.2501422295538305
1117	135-139	0.31968283871273684
1117	140-144	0.2572830655587879
1117	145-149	0.6958209934505817
1117	150-154	0.9348284045387913
1117	155-159	-0.19111182589517028
1117	160-164	-0.7683153411855059
1117	165-169	0.30669707383709266
1117	170-174	0.5637160727624391
1117	175-179	0.48984300260222113
1117	180-184	0.6099544609082841
1117	185-189	0.8454529182847708
1117	190-194	0.8647147630449155
1117	195-199	0.8725918474194785
1117	200-204	0.6734653060090068
1117	205-209	1.4255406344606811
1117	210-214	1.1801335692534423
1117	215-219	0.7391600651413412
1117	220-224	1.5094909826863692
1117	225-229	0.6834240448162952
1117	230-234	1.3670874699173368
1117	235-239	1.0480457218863997
1117	240-244	1.0351727631779077
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2108	200-204	-0.90121810034249
2108	205-209	-0.5479962778521497
2108	210-214	0.13001405197247706
2108	215-219	-0.88915208929388
2108	220-224	-0.20679315602632897
2108	225-229	0.07711832760362825
2108	230-234	-0.24462013777536384
2108	235-239	-0.5837313438735343
2108	240-244	-0.3805947993234575
2108	245-249	0.4376241666822054
2108	250-254	-0.6352790766148289
2108	255-259	-0.5135856543541095
2108	260-264	0.19830836730009338
2108	265-269	-0.761104372944029
2108	270-274	-0.5004639916648692
2108	275-279	0.03678165433155911
2108	280-284	-0.5019441827993845
2108	285-289	-0.11096760034898878
2108	290-294	-0.38771883510614913
2108	295-299	0.0708445582667725
2108	300-301	-0.25339039214043524
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	22.0
15	72.0
16	160.0
17	332.0
18	741.0
19	716.0
20	716.0
21	1023.0
22	1455.0
23	2110.0
24	3283.0
25	4674.0
26	6662.0
27	9285.0
28	12793.0
29	17718.0
30	25002.0
31	34302.0
32	45220.0
33	52282.0
34	43820.0
35	17971.0
36	1564.0
37	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.03916888054963	24.096150503843113	15.477790822642259	31.386889792964993
2	41.8439842090142	24.670400414282824	18.44161556114396	15.043999815559017
3	40.81253347426516	23.432517654903826	20.05263661933864	15.702312251492376
4	41.25732000156066	23.08314386751462	19.82598702528615	15.833549105638573
5	27.771137114137044	23.20622275505173	33.77220829062224	15.25043184018898
6	26.227507954017444	23.225730936073465	34.29715570720703	16.24960540270206
7	17.76734188619282	45.311829406277376	21.131261682740227	15.789567024789578
8	25.79087939333104	21.973660408678658	35.41266896744971	16.82279123054059
9	16.317706689177925	20.33426381445237	37.03362146325546	26.31440803311425
10-14	25.786906818286614	33.653101978129556	23.877304182199317	16.682687021384513
15-19	21.405865932686137	34.521960891417464	22.65595017255873	21.416223003337674
20-24	24.71509188353261	30.572866603058173	26.721951669368256	17.99008984404096
25-29	19.63863754863744	26.23091301834124	36.220094845229184	17.910354587792135
30-34	20.01234335817375	28.31573458942373	30.836546271631914	20.835375780770608
35-39	21.040885600479548	29.89887668346735	27.654584599887205	21.405653116165897
40-44	20.71719167319895	29.064139352257452	28.78740693710917	21.431262037434426
45-49	21.378199785055315	30.19788389440044	26.544320813810373	21.87959550673387
50-54	22.30317132084573	29.389252056339625	27.755459630479585	20.55211699233506
55-59	21.727502633604438	27.127438079259964	29.387833279538043	21.75722600759755
60-64	22.203998822415254	26.25120152660384	28.414694271333968	23.13010537964694
65-69	20.820407685513935	27.4077174364122	28.972131676674955	22.799743201398915
70-74	21.07841224688135	26.604476950197387	29.254326382509323	23.06278442041194
75-79	20.717759183919586	26.301426225379785	30.07799725466689	22.90281733603374
80-84	23.875176016996946	25.566357964480922	27.62025020128896	22.938215817233175
85-89	21.297897018085855	28.32424725023321	26.111735767008476	24.26611996467246
90-94	20.827714386042075	30.28996250882302	28.310556054097958	20.57176705103695
95-99	22.18307186459194	30.0657957741733	26.309371375468636	21.441760985766123
100-104	22.702982623531124	28.8813299613738	26.28376245420011	22.131924960894963
105-109	23.367679555071593	27.822000262473708	24.477092075067482	24.333228107387217
110-114	21.787375014631134	28.50854635675852	28.661135801768506	21.042942826841838
115-119	23.19622037860059	28.12625694757265	26.762670563573614	21.914852110253143
120-124	19.468029638247387	28.886011924819016	27.746237581269305	23.899720855664288
125-129	21.192765657088742	25.762858551499825	30.08523301635495	22.959142775056485
130-134	23.881773329124297	29.464730982183713	26.005185629209777	20.648310059482217
135-139	23.705703128048153	28.208829757424635	25.030202211163644	23.055264903363565
140-144	21.31591548346593	29.842834999804918	26.377401723104427	22.463847793624726
145-149	20.694349366693505	27.721905559122202	28.518974366250138	23.064770707934155
150-154	22.978580017238137	26.390738225039286	25.994828558558243	24.63585319916434
155-159	21.698559586852195	27.73226262977374	29.148769388471727	21.420408394902335
160-164	24.10267687713038	29.32647118286969	26.296602384254413	20.274249555745513
165-169	20.318657269634986	26.38584344507383	27.914575448776834	25.380923836514352
170-174	21.861009530633165	29.38095221205038	26.245597358237593	22.512440899078857
175-179	20.801325137532675	29.294548704834128	27.933232363717618	21.970893793915575
180-184	19.71241394231963	29.90639620051573	26.52779206407196	23.853397793092686
185-189	21.70473126593907	28.16484767657564	27.42658716787322	22.70383388961207
190-194	20.270702613741562	28.553450642528546	26.690100130172773	24.485746613557122
195-199	20.702294516782356	30.419922463847794	27.014006873973607	21.863776145396248
200-204	21.665714903895605	28.603462524784256	27.004572008243095	22.726250563077045
205-209	20.91227348341627	28.39724331667453	27.94259629060805	22.747886909301148
210-214	22.46434436550528	28.37440101016908	26.806581705582534	22.354672918743105
215-219	21.900593403397263	28.166833964097854	27.020675124941036	22.911897507563854
220-224	19.393047284283853	28.49967900174864	27.16439721494114	24.942876499026365
225-229	20.513526263332068	29.507861796951758	25.99255851567571	23.986053424040463
230-234	20.408111146974637	28.360922630554068	27.255766440962926	23.975199781508373
235-239	20.436415744166165	28.86182178035207	25.415045418592356	25.2867170568894
240-244	21.44105159736533	32.02867347915994	23.337885242238404	23.19238968123632
245-249	19.652257805932617	30.649977122224076	24.239234144282506	25.4585309275608
250-254	19.990848889629806	28.9293555561073	25.006295822057012	26.073499732205878
255-259	19.912816165542875	29.856171501739777	25.896649203888867	24.334363128828482
260-264	19.60558004916062	30.79965807479082	25.285794851968372	24.30896702408019
265-269	18.800495153103753	30.31330138720902	25.470803346894478	25.415400112792756
270-274	17.93121060677537	32.34477695055208	23.98569872984007	25.738313712832483
275-279	18.706288373478806	31.47542146538362	23.046539426033842	26.771750735103726
280-284	16.78278172473602	32.27433468235361	25.184919821376	25.75796377153437
285-289	17.9798037122295	31.264662171508835	25.491872182397945	25.26366193386372
290-294	17.53813849389749	33.37168759953606	24.082884940748333	25.00728896581812
295-299	16.569184877258074	34.549272344847886	21.977562044883005	26.903980733011036
300-301	12.573200015606545	38.14665186409537	25.965211592825245	23.31493652747284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.25
24	0.25
25	0.0
26	0.0
27	0.0
28	0.25
29	0.5
30	0.75
31	0.5
32	2.75
33	7.0
34	18.25
35	52.5
36	158.5
37	465.5
38	1368.75
39	2663.75
40	4755.25
41	6474.75
42	9012.5
43	12645.75
44	14687.75
45	11806.75
46	8895.75
47	6858.25
48	5563.0
49	3813.5
50	2980.75
51	1230.5
52	249.75
53	63.75
54	24.75
55	10.75
56	9.0
57	6.75
58	4.25
59	3.5
60	3.0
61	3.75
62	2.5
63	0.75
64	1.75
65	0.75
66	0.5
67	0.25
68	0.0
69	0.0
70	0.0
71	0.0
72	0.25
73	0.0
74	0.0
75	0.25
76	0.25
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.25
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	281933.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.44111549708488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	31.488604302297922	3.6026475866459435
2	6.669592497120899	1.5261515615610188
3	6.2092275239582575	2.131214677478544
4	6.346573743615232	2.904475328458729
5	6.117903448467983	3.4997819976968025
6	5.743786012955938	3.942919149886173
7	5.156713566160013	4.1298988847074805
8	4.527154270617526	4.143655590660494
9	3.9671644183238612	4.084990767537196
>10	23.308473629632648	44.91511719435856
>50	0.3469536839308577	2.5710208176700613
>100	0.04345648800243369	0.8303203951927021
>500	0.024798804972165425	1.909121267836741
>1k	0.03719820745821222	9.889269446836357
>5k	0.01239940248607074	9.919415333473177
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	9017	3.198277604962881	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	7645	2.711637162020764	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	5678	2.0139536698435445	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	5629	1.9965736540241832	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	4298	1.5244756732982658	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4246	1.5060315748777193	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3043	1.0793344518023784	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	2744	0.9732808858842349	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	2722	0.9654776134755421	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2034	0.7214480036036931	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	1991	0.7061961529867026	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1824	0.6469622215207159	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1373	0.4869951371425126	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1251	0.4437224446943068	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1244	0.4412395852915409	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1114	0.3951293392401741	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT	830	0.29439618632795733	TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	824	0.2922680211255866	No Hit
GGGGGGAGCGAAAGCAGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGG	760	0.2695675923002983	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	705	0.2500594112785662	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	612	0.21707285064181914	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	609	0.2160087680406338	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA	531	0.18834262040981367	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	512	0.18160343060230622	No Hit
GGGGGGAGCAAAAGCAGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGG	291	0.10321601231498263	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	3.546942003951293E-4	0.0	0.0
8	3.546942003951293E-4	0.0	0.0
9	3.546942003951293E-4	0.0	0.0
10-14	9.931437611063621E-4	0.0	0.0
15-19	0.0017734710019756468	0.0	0.0
20-24	0.0024828594027659053	0.0	0.0
25-29	0.0033341254837142157	0.0	0.0
30-34	0.006100740246796224	0.0	0.0
35-39	0.008157966609087975	0.0	0.0
40-44	0.00893829384995726	0.0	0.0
45-49	0.010215192971379725	0.0	0.0
50-54	0.01440058453604225	0.0	0.0
55-59	0.021991040424498016	0.0	0.0
60-64	0.03199341687564067	0.0	0.0
65-69	0.04639400141168292	0.0	0.0
70-74	0.06597312127349406	0.0	0.0
75-79	0.09292988050352388	0.0	0.0
80-84	0.13563506223109748	0.0	0.0
85-89	0.19203144009392303	0.0	0.0
90-94	0.2648146900150036	0.0	0.0
95-99	0.3634906165649286	0.0	0.0
100-104	0.48564729918101107	0.0	0.0
105-109	0.6356829459481508	0.0	0.0
110-114	0.8188470310321957	0.0	0.0
115-119	1.0468444630461846	0.0	0.0
120-124	1.3122266637818203	0.0	0.0
125-129	1.6310258110969627	0.0	0.0
130-134	2.030624297262116	0.0	0.0
135-139	2.4676784909889937	0.0	0.0
140-144	2.9658819648639927	0.0	0.0
145-149	3.527008189889087	0.0	0.0
150-154	4.162974891197554	0.0	0.0
155-159	4.92641868812803	0.0	0.0
160-164	5.79222723129254	0.0	0.0
165-169	6.735004415942795	0.0	0.0
170-174	7.811146619941617	0.0	0.0
175-179	8.987525404972104	0.0	0.0
180-184	10.2592459910688	0.0	0.0
185-189	11.726403081583214	0.0	0.0
190-194	13.310751135908177	0.0	0.0
195-199	14.928227628550044	0.0	0.0
200-204	16.68020416198175	0.0	0.0
205-209	18.54561190069981	0.0	0.0
210-214	20.508773361046774	0.0	0.0
215-219	22.50634015883206	0.0	0.0
220-224	24.578108983340012	0.0	0.0
225-229	26.700811895024707	0.0	0.0
230-234	28.844016131492232	0.0	0.0
235-239	30.914720873399	0.0	0.0
240-244	32.88455058471339	0.0	0.0
245-249	34.68675181692104	0.0	0.0
250-254	36.57436341258384	0.0	0.0
255-259	38.29030301525539	0.0	0.0
260-264	39.94793089138199	0.0	0.0
265-269	41.39749514955681	0.0	0.0
270-274	42.690639265357376	0.0	0.0
275-279	43.86652147850731	0.0	0.0
280-284	44.89378682169168	0.0	0.0
285-289	45.77960011775848	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	3900	0.0	293.10898	1
GGGTTAT	3915	0.0	291.98596	2
GGTTATT	3965	0.0	287.93192	3
GTTATTA	3985	0.0	286.48682	4
GGGGGGA	2765	0.0	285.9313	1
TTATTAG	4115	0.0	277.4362	5
TTAGTAG	4150	0.0	275.09637	8
TATTAGT	4170	0.0	274.1307	6
TAGTAGA	4210	0.0	270.8254	9
GGGGAGC	2920	0.0	270.2483	3
ATTAGTA	4230	0.0	270.2423	7
GGAGCGA	2095	0.0	269.65393	5
GGGAGCG	2120	0.0	266.47406	4
CGAAAGC	2200	0.0	256.11365	9
AGCGAAA	2345	0.0	240.90619	7
GAGCGAA	2350	0.0	240.39362	6
AAACGTT	130	0.0	238.26921	295
GCGAAAG	2385	0.0	236.86581	8
GGGGGAG	3555	0.0	222.39099	2
GGGAGCA	1030	0.0	216.23787	4
>>END_MODULE