FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_6957_flu.3510000003b994.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_6957_flu.3510000003b994.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281933
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC90173.198277604962881No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT76452.711637162020764No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG56782.0139536698435445No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA56291.9965736540241832No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG42981.5244756732982658No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA42461.5060315748777193No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT30431.0793344518023784No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT27440.9732808858842349No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA27220.9654776134755421No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG20340.7214480036036931No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA19910.7061961529867026No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT18240.6469622215207159No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT13730.4869951371425126No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA12510.4437224446943068No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG12440.4412395852915409No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT11140.3951293392401741No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT8300.29439618632795733TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT8240.2922680211255866No Hit
GGGGGGAGCGAAAGCAGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGG7600.2695675923002983No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA7050.2500594112785662No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6120.21707285064181914No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6090.2160087680406338No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA5310.18834262040981367No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA5120.18160343060230622No Hit
GGGGGGAGCAAAAGCAGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGG2910.10321601231498263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA39000.0293.108981
GGGTTAT39150.0291.985962
GGTTATT39650.0287.931923
GTTATTA39850.0286.486824
GGGGGGA27650.0285.93131
TTATTAG41150.0277.43625
TTAGTAG41500.0275.096378
TATTAGT41700.0274.13076
TAGTAGA42100.0270.82549
GGGGAGC29200.0270.24833
ATTAGTA42300.0270.24237
GGAGCGA20950.0269.653935
GGGAGCG21200.0266.474064
CGAAAGC22000.0256.113659
AGCGAAA23450.0240.906197
GAGCGAA23500.0240.393626
AAACGTT1300.0238.26921295
GCGAAAG23850.0236.865818
GGGGGAG35550.0222.390992
GGGAGCA10300.0216.237874
Produced by FastQC (version 0.11.2)