##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_6955_flu.3510000003bb42.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406625
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.808624654165385	34.0	34.0	34.0	34.0	34.0
2	33.878905625576394	34.0	34.0	34.0	34.0	34.0
3	33.88494804795573	34.0	34.0	34.0	34.0	34.0
4	33.89255210574854	34.0	34.0	34.0	34.0	34.0
5	33.880029511220414	34.0	34.0	34.0	34.0	34.0
6	37.6446922840455	38.0	38.0	38.0	38.0	38.0
7	37.65025023055641	38.0	38.0	38.0	38.0	38.0
8	37.65978235474946	38.0	38.0	38.0	38.0	38.0
9	37.574603135567166	38.0	38.0	38.0	37.0	38.0
10-14	37.64977706732247	38.0	38.0	38.0	37.6	38.0
15-19	37.61556618505994	38.0	38.0	38.0	37.2	38.0
20-24	37.60790359667999	38.0	38.0	38.0	37.0	38.0
25-29	37.51850378112512	38.0	38.0	38.0	37.0	38.0
30-34	37.44978690439594	38.0	38.0	38.0	37.0	38.0
35-39	37.37513581309561	38.0	38.0	38.0	37.0	38.0
40-44	37.36661789117737	38.0	38.0	38.0	37.0	38.0
45-49	37.34055161389487	38.0	38.0	38.0	37.0	38.0
50-54	37.2951377805103	38.0	38.0	38.0	37.0	38.0
55-59	37.24646295727021	38.0	38.0	38.0	37.0	38.0
60-64	37.06260756225023	38.0	38.0	38.0	36.4	38.0
65-69	37.03079495849985	38.0	38.0	38.0	36.2	38.0
70-74	37.11418235474946	38.0	38.0	38.0	36.8	38.0
75-79	36.9966922840455	38.0	38.0	38.0	36.2	38.0
80-84	36.89163774976945	38.0	38.0	38.0	36.0	38.0
85-89	36.93546437134952	38.0	38.0	38.0	36.0	38.0
90-94	36.78125545650169	38.0	38.0	38.0	35.4	38.0
95-99	36.76932554565017	38.0	38.0	38.0	35.4	38.0
100-104	36.76369086996618	38.0	38.0	38.0	35.4	38.0
105-109	36.584823363049495	38.0	38.0	38.0	34.8	38.0
110-114	36.49798118659699	38.0	37.0	38.0	34.0	38.0
115-119	36.55546609283738	38.0	37.2	38.0	34.0	38.0
120-124	36.468570304334456	38.0	37.0	38.0	34.0	38.0
125-129	36.40299145404242	38.0	37.0	38.0	34.0	38.0
130-134	36.28184051644636	38.0	37.0	38.0	34.0	38.0
135-139	36.03861002151859	38.0	37.0	38.0	32.4	38.0
140-144	35.96760553335383	38.0	37.0	38.0	32.0	38.0
145-149	35.544864678758074	38.0	36.2	38.0	29.4	38.0
150-154	35.231396495542576	38.0	36.2	38.0	28.0	38.0
155-159	35.200055579465115	38.0	35.8	38.0	28.2	38.0
160-164	35.17980055333538	38.0	35.8	38.0	28.0	38.0
165-169	34.95492997233323	38.0	35.2	38.0	27.4	38.0
170-174	34.820944113126345	38.0	35.0	38.0	26.6	38.0
175-179	34.512658100215184	38.0	35.0	38.0	24.2	38.0
180-184	34.17230913003382	37.6	34.6	38.0	23.4	38.0
185-189	33.45900719335998	37.0	33.4	37.8	22.2	38.0
190-194	32.49844254534276	36.8	30.4	37.0	20.6	38.0
195-199	32.27776059022441	36.8	30.0	37.2	20.2	37.8
200-204	32.14822551490932	37.0	30.0	37.0	20.0	37.4
205-209	31.014444266830623	35.2	27.8	37.0	18.0	37.2
210-214	30.226452382416234	34.2	25.8	37.0	16.0	37.0
215-219	29.458886689209965	33.0	24.4	36.8	14.2	37.0
220-224	28.028284537350135	31.6	22.0	36.2	9.4	37.0
225-229	26.999466338764215	29.8	20.2	36.0	9.2	37.0
230-234	26.6487786043652	29.2	20.0	35.8	9.0	37.0
235-239	25.77063043344605	28.4	18.8	34.6	9.0	37.0
240-244	24.65340448816477	26.4	17.0	33.6	8.6	37.0
245-249	23.803248201660004	25.0	16.0	33.0	8.0	36.2
250-254	23.054755610205966	24.0	14.8	32.0	8.0	36.0
255-259	22.291119335997543	22.6	13.6	31.0	8.0	36.0
260-264	21.200622194897015	21.2	12.2	29.4	8.0	35.6
265-269	20.424685644020904	20.4	11.0	28.6	7.6	34.6
270-274	19.231758007992617	18.6	9.0	26.8	7.0	33.6
275-279	18.193260129111586	17.2	8.0	25.4	7.0	32.0
280-284	18.010345158315403	17.4	8.0	25.0	7.0	31.6
285-289	16.68545023055641	15.0	8.0	22.6	7.0	30.0
290-294	15.97049960036889	13.6	8.0	21.6	7.0	28.8
295-299	15.27252923455272	13.0	8.0	21.0	7.0	27.0
300-301	13.439163848754994	12.0	8.0	17.5	7.0	24.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.0664916558293811
1101	2	0.04310793375517363
1101	3	0.041861225251494716
1101	4	0.04104990161035715
1101	5	0.02105290058528908
1101	6	0.16832096519306106
1101	7	0.07029155531819953
1101	8	0.04983547104728103
1101	9	0.0559633314840724
1101	10-14	0.04640397762114645
1101	15-19	0.05795068392440328
1101	20-24	0.04761352073960268
1101	25-29	0.12222941861014647
1101	30-34	0.12897029582116204
1101	35-39	0.18563545473787002
1101	40-44	0.16379298160463662
1101	45-49	0.16247542624103772
1101	50-54	0.11396844764803404
1101	55-59	0.16408749348863694
1101	60-64	0.30330144318379126
1101	65-69	0.3217884676077958
1101	70-74	0.18055312493380882
1101	75-79	0.1969284165157461
1101	80-84	0.2524202203430619
1101	85-89	0.18624951030040648
1101	90-94	0.3795613942498903
1101	95-99	0.24982147352599782
1101	100-104	0.2946960811170527
1101	105-109	0.21521528571456372
1101	110-114	0.3018875925135589
1101	115-119	0.21292063085947888
1101	120-124	0.20555243684301416
1101	125-129	0.24358512117627384
1101	130-134	0.3437180636028998
1101	135-139	0.35992220413587717
1101	140-144	0.21396137662016912
1101	145-149	0.5360689235451233
1101	150-154	0.782465849717525
1101	155-159	0.4026444639429769
1101	160-164	0.451396428384534
1101	165-169	0.40223773090630743
1101	170-174	0.4386958500442262
1101	175-179	0.467741590763211
1101	180-184	0.3315713342861386
1101	185-189	0.25382949162817425
1101	190-194	0.7818035955775215
1101	195-199	0.3189581004809554
1101	200-204	0.054624004699846296
1101	205-209	-0.04253686879984642
1101	210-214	-0.33284333950973277
1101	215-219	-0.21384629711720393
1101	220-224	0.3520569228380275
1101	225-229	0.6181826393322112
1101	230-234	0.6904973424191212
1101	235-239	0.4422924895834335
1101	240-244	0.37060969565296276
1101	245-249	0.6835075649730875
1101	250-254	0.3106034266286386
1101	255-259	0.11880483731337321
1101	260-264	0.4108032386347702
1101	265-269	0.44502249893785617
1101	270-274	0.16651645290970052
1101	275-279	0.5720507293857473
1101	280-284	0.29866506421668504
1101	285-289	0.13110669526660246
1101	290-294	0.13348494322824855
1101	295-299	0.046298784445346186
1101	300-301	0.16328534798613603
1102	1	-0.012253804418818959
1102	2	-0.0014518403292456128
1102	3	0.06690307449574817
1102	4	0.045428246912337045
1102	5	0.016840721400186
1102	6	0.13167034659792876
1102	7	0.10468266639805535
1102	8	0.06351032703664572
1102	9	0.09464926521456363
1102	10-14	0.07166615082778094
1102	15-19	0.015411806328174293
1102	20-24	0.07382943470599201
1102	25-29	0.018669595291321173
1102	30-34	0.12963689449745175
1102	35-39	0.08546753024937459
1102	40-44	0.06202282890764366
1102	45-49	0.10660053086414933
1102	50-54	0.13378617484722355
1102	55-59	0.12434249253911389
1102	60-64	0.33152225245674316
1102	65-69	0.27159680510261097
1102	70-74	0.1958652007266508
1102	75-79	0.00931772702306688
1102	80-84	0.04805893124566296
1102	85-89	0.19362834091295156
1102	90-94	0.2807497017820566
1102	95-99	0.17174027895865862
1102	100-104	0.1641855203371705
1102	105-109	0.13860268311357515
1102	110-114	0.23702038473733467
1102	115-119	0.09399176050774116
1102	120-124	0.21716032700809507
1102	125-129	0.08947809261326967
1102	130-134	0.11242115904027372
1102	135-139	0.22979602338933347
1102	140-144	0.2987182289943249
1102	145-149	0.41886571908870707
1102	150-154	0.8549932275634546
1102	155-159	0.5672217162411641
1102	160-164	0.5526614009187654
1102	165-169	0.682665824057807
1102	170-174	0.3623856873596836
1102	175-179	0.5663897546715759
1102	180-184	0.7850861432918066
1102	185-189	0.8769907624385098
1102	190-194	0.9862185150089431
1102	195-199	0.7962561163344404
1102	200-204	0.40853638152592
1102	205-209	0.5285577878605068
1102	210-214	0.05255883524427318
1102	215-219	0.303020179176432
1102	220-224	1.071359798830624
1102	225-229	1.5740529629429183
1102	230-234	1.1000641148225334
1102	235-239	1.6131045759265135
1102	240-244	1.577169476770866
1102	245-249	1.5947896288793437
1102	250-254	1.1076358228671275
1102	255-259	1.0178982209126914
1102	260-264	1.745511735789723
1102	265-269	1.5876102592647747
1102	270-274	1.1732269264743458
1102	275-279	1.5998628930971748
1102	280-284	1.0990114634233095
1102	285-289	1.7697011936735194
1102	290-294	1.4734158040566516
1102	295-299	1.2124295580585205
1102	300-301	0.7336580172645686
1103	1	-0.0011439015625001048
1103	2	0.028090852490961993
1103	3	0.03122935162106444
1103	4	0.011082741274968555
1103	5	0.06873612796066197
1103	6	-0.021775423474636568
1103	7	0.08648203396297305
1103	8	0.09648360044054272
1103	9	0.1536241818449824
1103	10-14	0.06888716479519985
1103	15-19	0.03632117340978169
1103	20-24	0.05737024264870172
1103	25-29	0.036417304785445026
1103	30-34	0.1307728266142547
1103	35-39	-0.4972711691161109
1103	40-44	-0.338379865155332
1103	45-49	-0.1750787016524029
1103	50-54	0.09675609965924536
1103	55-59	-0.3428411345727369
1103	60-64	0.11128329417950766
1103	65-69	0.29590758204496126
1103	70-74	0.10363099952115817
1103	75-79	0.2678844261400002
1103	80-84	0.22942816010291978
1103	85-89	0.22793518239682697
1103	90-94	0.3260651253271405
1103	95-99	0.2780249299801554
1103	100-104	0.19832596787475865
1103	105-109	0.04080136162804848
1103	110-114	0.2486964412698498
1103	115-119	-0.03009013845454689
1103	120-124	0.28194988354405126
1103	125-129	0.2912247112259294
1103	130-134	0.40909880356480954
1103	135-139	0.44588825120404607
1103	140-144	0.3646269746877593
1103	145-149	0.6152395738357654
1103	150-154	0.9043423234436503
1103	155-159	0.015095533014502394
1103	160-164	-0.6749430785995898
1103	165-169	0.4383240812710838
1103	170-174	0.7903726658251315
1103	175-179	0.6491496847999301
1103	180-184	0.5920313644714739
1103	185-189	0.7148263983602661
1103	190-194	0.31184042101362053
1103	195-199	0.43963729655772
1103	200-204	0.4903897753410362
1103	205-209	0.8871427834072243
1103	210-214	0.6579081244452354
1103	215-219	0.3795570792779266
1103	220-224	1.1164710273150575
1103	225-229	1.8150751119794286
1103	230-234	0.8644953375073463
1103	235-239	1.0421306535171801
1103	240-244	1.1293622866191733
1103	245-249	1.3114079653541388
1103	250-254	1.325874429024399
1103	255-259	0.8051763335496567
1103	260-264	1.494256531663705
1103	265-269	0.3726134007272428
1103	270-274	0.5294551347360432
1103	275-279	1.233236616662289
1103	280-284	0.7426739944064131
1103	285-289	0.6507961486919172
1103	290-294	1.2490535899815196
1103	295-299	1.053964007128851
1103	300-301	-0.8674824026557548
2116	1	0.018796367740556263
2116	2	0.04879234490018547
2116	3	0.038189350934509037
2116	4	0.05793615218954784
2116	5	-0.015305649199206073
2116	6	-0.24083861271624585
2116	7	-0.13692192920309765
2116	8	0.10618125669724776
2116	9	0.07219476447674111
2116	10-14	0.12425837906191362
2116	15-19	0.11246682705756683
2116	20-24	-0.12085259747727406
2116	25-29	-0.2086513940980339
2116	30-34	0.019267630215253462
2116	35-39	-0.14479843841058937
2116	40-44	-0.1516680571861002
2116	45-49	0.05321391010664911
2116	50-54	0.22441965106111894
2116	55-59	0.12649659274290315
2116	60-64	0.33959547289099845
2116	65-69	0.25061163188919977
2116	70-74	-0.8604734262145328
2116	75-79	0.1950625798092389
2116	80-84	-0.16304886160094867
2116	85-89	0.06893155221222003
2116	90-94	0.32893254206032196
2116	95-99	0.19914453726798342
2116	100-104	0.2865957515226114
2116	105-109	0.3026761846243673
2116	110-114	0.3878723979479304
2116	115-119	0.29668517629018964
2116	120-124	-0.15301780523841302
2116	125-129	0.07550472461384317
2116	130-134	0.23379281247292738
2116	135-139	0.43320337354041527
2116	140-144	0.5797592661666897
2116	145-149	0.7690603645809446
2116	150-154	1.0510697506468603
2116	155-159	-0.31490884380381345
2116	160-164	-0.5643317829755503
2116	165-169	0.7254572652776758
2116	170-174	0.7961900580996186
2116	175-179	0.6694310026513435
2116	180-184	0.6897882021773469
2116	185-189	1.1738961099193617
2116	190-194	1.0561405162737785
2116	195-199	1.0196599530289774
2116	200-204	0.9349674123740641
2116	205-209	1.003473464903582
2116	210-214	0.8804897606887252
2116	215-219	1.332481566289072
2116	220-224	1.7273955658181848
2116	225-229	1.4968282422627155
2116	230-234	1.6834732217018846
2116	235-239	1.3585272447481032
2116	240-244	2.277678927968953
2116	245-249	1.6195067956279914
2116	250-254	1.6611749276384025
2116	255-259	1.5319197232667037
2116	260-264	0.6181060720977847
2116	265-269	0.9032816808092861
2116	270-274	1.2600230054568442
2116	275-279	1.6739961233691183
2116	280-284	0.09280178033709063
2116	285-289	-1.0036717246034836
2116	290-294	0.44348747857004334
2116	295-299	0.7402122156370439
2116	300-301	1.4328411386193594
1117	1	-0.13046658497355423
1117	2	0.03511128536207764
1117	3	0.0049482572851218265
1117	4	-0.01636030267391675
1117	5	-0.018535778508805834
1117	6	-0.5104534685264781
1117	7	-0.2151759886193787
1117	8	-0.04192434903901443
1117	9	0.05767484592612249
1117	10-14	0.092512960589616
1117	15-19	0.06117355707375083
1117	20-24	0.022759021242158894
1117	25-29	0.13469464394648156
1117	30-34	0.05773262264465018
1117	35-39	-0.1925966945584463
1117	40-44	-0.1191833289450841
1117	45-49	0.07406843246172201
1117	50-54	0.06621606241530031
1117	55-59	0.22059464526730466
1117	60-64	0.2729225408873148
1117	65-69	0.23817946286433767
1117	70-74	-0.10035194829114147
1117	75-79	0.18372336402059375
1117	80-84	0.036256730212350874
1117	85-89	0.040764337328575095
1117	90-94	0.24225752161095215
1117	95-99	0.221961502408341
1117	100-104	0.16421492345390476
1117	105-109	0.24660803168053036
1117	110-114	0.3149403942583362
1117	115-119	0.30671980968094914
1117	120-124	0.381389685320066
1117	125-129	0.41897606939881626
1117	130-134	0.2860829671919376
1117	135-139	0.39181629753839786
1117	140-144	0.33017991393293045
1117	145-149	0.8877488206974817
1117	150-154	1.017503903695129
1117	155-159	-0.08218812120160379
1117	160-164	-0.693804624479931
1117	165-169	0.29071492726274073
1117	170-174	0.6027498659626147
1117	175-179	0.8068834470029671
1117	180-184	0.5254821752711933
1117	185-189	0.7271594803226051
1117	190-194	0.9902789454145591
1117	195-199	0.7269235670842136
1117	200-204	0.8479547598740282
1117	205-209	1.3201775495552504
1117	210-214	0.8242628858736438
1117	215-219	0.11022639303510218
1117	220-224	0.9040697160818496
1117	225-229	0.7397412759040947
1117	230-234	1.211187243874214
1117	235-239	0.6842440167072361
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2108	185-189	-0.8934893051337411
2108	190-194	-0.48505774193175455
2108	195-199	-1.4057502808740523
2108	200-204	-0.9970826383995899
2108	205-209	-0.35973026652960627
2108	210-214	-0.3450633278983908
2108	215-219	-0.850398934907119
2108	220-224	-0.5041033756802342
2108	225-229	-0.5603039929828064
2108	230-234	-0.1735622854322436
2108	235-239	-0.5798896499697186
2108	240-244	-0.6732306949291065
2108	245-249	-0.20947033041114693
2108	250-254	-0.36776157947851473
2108	255-259	-0.9727607469489534
2108	260-264	0.015995265054872476
2108	265-269	-0.8625884419080485
2108	270-274	-1.034879319804336
2108	275-279	-0.19646978943788795
2108	280-284	-0.68628257277215
2108	285-289	-0.20924271305462838
2108	290-294	-0.6188666977780439
2108	295-299	0.16276776545546312
2108	300-301	-0.28586766157340904
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	20.0
14	108.0
15	294.0
16	393.0
17	638.0
18	1244.0
19	1192.0
20	1120.0
21	1411.0
22	2188.0
23	3143.0
24	4584.0
25	6521.0
26	8886.0
27	12318.0
28	17120.0
29	24014.0
30	34163.0
31	48688.0
32	65455.0
33	76610.0
34	65734.0
35	28232.0
36	2544.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.30919151552413	20.349707961881343	15.097940362742085	33.243160159852444
2	46.76274208422994	22.742084229941593	16.042299415923765	14.452874269904703
3	46.611497079618815	21.390962188748848	18.14718721180449	13.850353519827852
4	47.05932984936981	21.252505379649556	17.69861666154319	13.989548109437441
5	29.129541961266526	21.262342453120198	35.772517675991395	13.835597909621889
6	27.687918844143866	21.85551798339994	35.91589302182601	14.54067015063019
7	16.505625576391022	49.78936366430987	19.44789425146019	14.25711650783892
8	27.19631109744851	20.034183830310482	37.36071318782662	15.408791884414386
9	15.463141715339686	18.73396864432831	38.637565324316014	27.165324316015987
10-14	27.192031970488777	34.635302797417765	22.88782047340916	15.28484475868429
15-19	21.500891484783278	36.88843529050108	20.33992007377805	21.270753150937598
20-24	26.805976022133414	30.63869658776514	25.388109437442363	17.167217952659083
25-29	18.26159237626806	26.80179526590839	37.7952167230249	17.14139563479865
30-34	19.090267445434982	29.444820166000614	29.67151552413157	21.793396864432832
35-39	21.041696895173686	31.4740608668921	27.510802336304952	19.973439901629263
40-44	20.090107592991085	31.455468798032587	27.639225330464186	20.815198278512142
45-49	20.624900092222564	29.912228711958193	26.88629572702121	22.576575468798033
50-54	22.822354749462033	30.555327390101443	25.509302182600674	21.113015677835843
55-59	21.74529357516139	26.95963110974485	29.615050722410086	21.68002459268368
60-64	20.507199508146325	28.15281893636643	28.373857977251767	22.966123578235475
65-69	19.13905932984937	28.924488164770978	28.442766676913617	23.49368582846603
70-74	20.821936673839534	26.88968951736858	30.10778973255456	22.18058407623732
75-79	21.660645557946513	25.895456501690745	28.31281893636643	24.131079003996312
80-84	23.171620043037198	28.32354134644943	25.360122963418384	23.14471564709499
85-89	21.447722102674454	28.386203504457423	26.174288349216106	23.991786043652013
90-94	22.422723639717184	30.23636028281586	27.49535813095604	19.845557946510915
95-99	24.401106670765447	27.818014140793114	27.457928066400246	20.322951122041193
100-104	23.007340916077467	27.51326160467261	27.706855210574854	21.772542268675068
105-109	25.25653857977252	28.255911466338762	24.23869658776514	22.248853366123576
110-114	21.1031786043652	27.36172148785736	29.94311712265601	21.591982785121427
115-119	24.254091607746695	26.567377805102982	27.964438979403628	21.214091607746695
120-124	20.140768521364894	28.4953458346142	27.896858284660315	23.46702735936059
125-129	20.390039963110976	25.68268060252075	29.370845373501382	24.55643406086689
130-134	23.64861973562865	29.00229941592376	26.226916692284046	21.122164156163542
135-139	22.378260067629878	27.022588379956964	25.841992007377808	24.75715954503535
140-144	22.222490009222255	28.501051337227175	26.50215800799262	22.774300645557947
145-149	22.76485705502613	24.909093144789427	28.88868121733784	23.437368582846606
150-154	21.520122963418384	27.64960344297571	26.219538887181066	24.61073470642484
155-159	22.29110359667999	27.735038426068243	29.464740239778664	20.5091177374731
160-164	23.295763910236705	29.777755917614513	24.725680909929295	22.20079926221949
165-169	20.316261912081156	28.646394097755916	27.47735628650477	23.559987703658162
170-174	21.38609283738088	29.72571779895481	27.245398094067014	21.642791269597296
175-179	20.03260989855518	28.26865047648325	28.19324930833077	23.5054903166308
180-184	21.287918844143867	28.282078081770674	28.387383953273904	22.04261912081156
185-189	21.081881340301262	27.382576083615124	28.486344912388567	23.04919766369505
190-194	20.1898555179834	26.779514294497385	29.12614817091915	23.904482016600063
195-199	18.857423916384874	32.34592068859514	27.068970181371043	21.72768521364894
200-204	20.47985244389794	28.036298801106668	26.209505072241008	25.27434368275438
205-209	22.209947740547186	25.752474638794958	28.92340608668921	23.114171533968644
210-214	22.744395942207195	26.98392868121734	28.78303104826314	21.488644328312326
215-219	21.896932062711343	27.625896095911468	29.29141100522595	21.185760836151246
220-224	20.391171226560097	27.051460190593296	27.79204426683062	24.765324316015985
225-229	22.75644635720873	27.200983707347064	28.50395327390102	21.53861666154319
230-234	21.365582539194587	27.849099292960343	27.887857362434676	22.89746080541039
235-239	21.29042729787888	26.905674761758373	26.79279434368275	25.01110359667999
240-244	22.14492468490624	29.765213648939444	26.11369197663695	21.97616968951737
245-249	22.51268367660621	27.06341223486013	26.526209652628342	23.89769443590532
250-254	19.958733476790655	26.927414694128498	25.50753150937596	27.606320319704885
255-259	21.715683984014756	28.416452505379645	25.246062096526284	24.62180141407931
260-264	19.625158315401166	26.698948662772825	28.3920073778051	25.283885644020902
265-269	20.796507838917922	27.036606209652632	26.49600983707347	25.670876114355977
270-274	19.408595142944975	30.348404549646478	25.22520750076852	25.017792806640028
275-279	20.47754073163234	28.22861358745773	25.70412542268675	25.58972025822318
280-284	17.430851521672302	29.02182600676299	28.519446664617277	25.02787580694743
285-289	19.68447586842914	28.19723332308638	26.197257915770056	25.92103289271442
290-294	20.402287119581924	28.25886258837996	26.477417768213957	24.861432523824163
295-299	18.884820166000615	28.25689517368583	25.802791269597297	27.05549339071626
300-301	15.494251460190595	30.598217030433446	28.35671687672917	25.55081463264679
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	0.25
29	1.0
30	0.5
31	1.25
32	5.5
33	25.75
34	122.5
35	268.25
36	455.0
37	1052.75
38	2605.75
39	5056.75
40	7584.25
41	8533.5
42	10426.0
43	13639.5
44	16385.0
45	16268.75
46	14856.0
47	13558.25
48	9440.25
49	6873.0
50	5166.25
51	2105.5
52	553.75
53	130.0
54	58.25
55	55.25
56	62.0
57	85.5
58	95.5
59	146.5
60	152.5
61	82.0
62	28.25
63	10.0
64	2.75
65	2.5
66	1.25
67	1.5
68	1.0
69	0.5
70	0.0
71	0.25
72	0.25
73	0.0
74	0.0
75	0.0
76	0.0
77	0.25
78	0.25
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.25
97	0.25
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	406625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.210994506075181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	34.09490949699832	3.1404802406198202
2	8.097630217833094	1.4917445489737802
3	6.4518091745041195	1.7828273658180869
4	5.7873588667523	2.1322932290536367
5	4.869494911324111	2.242644543778372
6	4.662521731615297	2.576787723261878
7	3.823885844942379	2.465525405673603
8	3.2477713194167306	2.393216302410884
9	3.0400801870047354	2.520194571047576
>10	24.761051588576716	43.91101062828218
>50	0.9647412347557138	5.682444370851441
>100	0.1292801155030737	1.8854232585519597
>500	0.016030456336910148	0.9733020681935255
>1k	0.024045684496399877	5.5553276563528495
>5k	0.021373941774577666	12.353922581240056
>10k+	0.008015228165466626	8.892855505890253
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	13135	3.230249000922226	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	12350	3.0371964340608666	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	10651	2.6193667383953274	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	8591	2.1127574546572396	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	6225	1.5308945588687366	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	6217	1.5289271441746082	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	6039	1.485152167230249	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	6006	1.477036581616969	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	5874	1.4445742391638488	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTAAATGAGTGACATCGAAGC	5664	1.3929296034429757	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA	5584	1.3732554565016908	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	4973	1.2229941592376268	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	3614	0.8887795880725484	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	3579	0.8801721487857362	No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	2732	0.6718721180448817	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	2689	0.661297264063941	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT	1373	0.33765754687980326	TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	1334	0.32806640024592687	No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTAAATGAGTGACATCGAAGC	1184	0.29117737473101757	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	1096	0.26953581309560404	No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	840	0.20657854288349217	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	765	0.1881340301260375	No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	633	0.1556716876729173	No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG	626	0.15395019981555486	No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	574	0.14116200430371964	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	517	0.1271441746080541	No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	420	0.10328927144174609	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	2.459268367660621E-4	0.0	0.0
4	2.459268367660621E-4	0.0	0.0
5	2.459268367660621E-4	0.0	0.0
6	2.459268367660621E-4	0.0	0.0
7	2.459268367660621E-4	0.0	0.0
8	2.459268367660621E-4	0.0	0.0
9	2.459268367660621E-4	0.0	0.0
10-14	0.00142637565324316	0.0	0.0
15-19	0.0018198585920688594	0.0	0.0
20-24	0.0025576391023670454	0.0	0.0
25-29	0.002705195204426683	0.0	0.0
30-34	0.004918536735321242	0.0	0.0
35-39	0.004967722102674455	0.0	0.0
40-44	0.005557946510913003	0.0	0.0
45-49	0.008705810021518597	0.0	0.0
50-54	0.011460190593298494	0.0	0.0
55-59	0.013378419920073778	0.0	0.0
60-64	0.016772210267445437	0.0	0.0
65-69	0.020608668920996002	0.0	0.0
70-74	0.027592991085152164	0.0	0.0
75-79	0.03836458653550569	0.0	0.0
80-84	0.05405471872118045	0.0	0.0
85-89	0.07003996311097449	0.0	0.0
90-94	0.08986166615431909	0.0	0.0
95-99	0.10978173993237013	0.0	0.0
100-104	0.1374239163848755	0.0	0.0
105-109	0.16959114663387642	0.0	0.0
110-114	0.209775591761451	0.0	0.0
115-119	0.26097755917614507	0.0	0.0
120-124	0.3228035659391331	0.0	0.0
125-129	0.39899169996925915	0.0	0.0
130-134	0.48998462957270217	0.0	0.0
135-139	0.5963233937903474	0.0	0.0
140-144	0.7216477098063325	0.0	0.0
145-149	0.867334767906548	0.0	0.0
150-154	1.0508453735013834	0.0	0.0
155-159	1.2805902244082386	0.0	0.0
160-164	1.5345834614202274	0.0	0.0
165-169	1.8576329541961265	0.0	0.0
170-174	2.2381309560405778	0.0	0.0
175-179	2.6743559790962186	0.0	0.0
180-184	3.1551921303412236	0.0	0.0
185-189	3.7214140793114048	0.0	0.0
190-194	4.361512450046112	0.0	0.0
195-199	5.078094067015064	0.0	0.0
200-204	5.867076544727944	0.0	0.0
205-209	6.781629265293576	0.0	0.0
210-214	7.805668613587457	0.0	0.0
215-219	8.760897632954196	0.0	0.0
220-224	9.829744850906854	0.0	0.0
225-229	10.980338149400554	0.0	0.0
230-234	12.205644020903781	0.0	0.0
235-239	13.348416845988316	0.0	0.0
240-244	14.561032892714417	0.0	0.0
245-249	15.69101752228712	0.0	0.0
250-254	16.8673839532739	0.0	0.0
255-259	18.339108515216726	0.0	0.0
260-264	19.680836151245007	0.0	0.0
265-269	20.749289886258833	0.0	0.0
270-274	21.77195204426683	0.0	0.0
275-279	22.663289271441744	0.0	0.0
280-284	23.48753765754688	0.0	0.0
285-289	24.262797417768212	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCCGC	10	8.463019E-4	295.0	295
CGGGTTA	7185	0.0	293.35767	1
GGGTTAT	7280	0.0	289.52954	2
GGGGGGA	4795	0.0	287.30972	1
ATTAGTA	7585	0.0	277.69284	7
GGTTATT	7615	0.0	276.7925	3
TTATTAG	7700	0.0	273.73703	5
GGAGCGA	4145	0.0	271.5139	5
TTAGTAG	7795	0.0	270.21167	8
CGAAAGC	4195	0.0	268.2777	9
GAGCGAA	4195	0.0	268.2777	6
GCGAAAG	4220	0.0	267.0379	8
TATTAGT	7970	0.0	264.46362	6
GTTATTA	8015	0.0	262.97882	4
TAGTAGA	8015	0.0	262.24268	9
GGGGGAG	5360	0.0	257.02426	2
GGGAGCG	4875	0.0	231.15897	4
GGGGAGC	6130	0.0	224.49835	3
AGCGAAA	5200	0.0	216.9952	7
GGCCGGC	25	5.5538723E-5	177.00002	295
>>END_MODULE