FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_6955_flu.3510000003bb42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_6955_flu.3510000003bb42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406625
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA131353.230249000922226No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC123503.0371964340608666No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC106512.6193667383953274No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG85912.1127574546572396No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG62251.5308945588687366No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA62171.5289271441746082No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG60391.485152167230249No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG60061.477036581616969No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT58741.4445742391638488No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTAAATGAGTGACATCGAAGC56641.3929296034429757No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA55841.3732554565016908No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA49731.2229941592376268No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA36140.8887795880725484No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT35790.8801721487857362No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA27320.6718721180448817No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA26890.661297264063941No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT13730.33765754687980326TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG13340.32806640024592687No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTAAATGAGTGACATCGAAGC11840.29117737473101757No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG10960.26953581309560404No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA8400.20657854288349217No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT7650.1881340301260375No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA6330.1556716876729173No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG6260.15395019981555486No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA5740.14116200430371964No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG5170.1271441746080541No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA4200.10328927144174609No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCGC108.463019E-4295.0295
CGGGTTA71850.0293.357671
GGGTTAT72800.0289.529542
GGGGGGA47950.0287.309721
ATTAGTA75850.0277.692847
GGTTATT76150.0276.79253
TTATTAG77000.0273.737035
GGAGCGA41450.0271.51395
TTAGTAG77950.0270.211678
CGAAAGC41950.0268.27779
GAGCGAA41950.0268.27776
GCGAAAG42200.0267.03798
TATTAGT79700.0264.463626
GTTATTA80150.0262.978824
TAGTAGA80150.0262.242689
GGGGGAG53600.0257.024262
GGGAGCG48750.0231.158974
GGGGAGC61300.0224.498353
AGCGAAA52000.0216.99527
GGCCGGC255.5538723E-5177.00002295
Produced by FastQC (version 0.11.2)