##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_6929_flu.3510000003bb85.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	397083
Sequences flagged as poor quality	0
Sequence length	301
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.830944663961944	34.0	34.0	34.0	34.0	34.0
2	33.89230714989058	34.0	34.0	34.0	34.0	34.0
3	33.89330190413591	34.0	34.0	34.0	34.0	34.0
4	33.89860306283573	34.0	34.0	34.0	34.0	34.0
5	33.88638395499178	34.0	34.0	34.0	34.0	34.0
6	37.62946033952599	38.0	38.0	38.0	38.0	38.0
7	37.646947867322446	38.0	38.0	38.0	38.0	38.0
8	37.66271031497193	38.0	38.0	38.0	38.0	38.0
9	37.58583218118127	38.0	38.0	38.0	37.0	38.0
10-14	37.65446317268682	38.0	38.0	38.0	38.0	38.0
15-19	37.62682260383849	38.0	38.0	38.0	37.6	38.0
20-24	37.59832075409927	38.0	38.0	38.0	37.0	38.0
25-29	37.5082257361811	38.0	38.0	38.0	37.0	38.0
30-34	37.45642497916053	38.0	38.0	38.0	37.0	38.0
35-39	37.399354794841386	38.0	38.0	38.0	37.0	38.0
40-44	37.38005253309762	38.0	38.0	38.0	37.0	38.0
45-49	37.36708043406542	38.0	38.0	38.0	37.0	38.0
50-54	37.32794453552532	38.0	38.0	38.0	37.0	38.0
55-59	37.267698692716635	38.0	38.0	38.0	37.0	38.0
60-64	37.1036292160581	38.0	38.0	38.0	36.4	38.0
65-69	37.021334582442464	38.0	38.0	38.0	36.2	38.0
70-74	37.11528773581342	38.0	38.0	38.0	36.6	38.0
75-79	37.0120161276106	38.0	38.0	38.0	36.0	38.0
80-84	36.9017792249983	38.0	38.0	38.0	35.8	38.0
85-89	36.93586227564514	38.0	38.0	38.0	36.0	38.0
90-94	36.77201290410317	38.0	38.0	38.0	35.2	38.0
95-99	36.780992386982064	38.0	38.0	38.0	35.2	38.0
100-104	36.73765484797889	38.0	38.0	38.0	35.2	38.0
105-109	36.57467280140425	38.0	38.0	38.0	34.2	38.0
110-114	36.50003097589169	38.0	37.2	38.0	34.0	38.0
115-119	36.562945278443046	38.0	37.4	38.0	34.0	38.0
120-124	36.43204619689082	38.0	37.0	38.0	34.0	38.0
125-129	36.34183634152053	38.0	37.0	38.0	34.0	38.0
130-134	36.21950322728498	38.0	37.0	38.0	33.6	38.0
135-139	35.96061730167245	38.0	37.0	38.0	31.8	38.0
140-144	35.857569324297444	38.0	37.0	38.0	31.4	38.0
145-149	35.3679160276315	38.0	36.2	38.0	28.2	38.0
150-154	34.97291196047174	38.0	35.6	38.0	27.0	38.0
155-159	35.102948753787	38.0	35.6	38.0	27.6	38.0
160-164	35.00125918258903	38.0	35.8	38.0	27.2	38.0
165-169	34.69614513842195	38.0	35.2	38.0	25.2	38.0
170-174	34.6059161434763	38.0	35.0	38.0	24.2	38.0
175-179	34.21111656756899	37.8	34.4	38.0	23.4	38.0
180-184	33.90291500769361	37.2	34.0	38.0	22.8	38.0
185-189	33.03779763928448	37.0	32.4	37.8	21.2	38.0
190-194	31.962236106808906	36.6	29.4	37.0	19.4	38.0
195-199	31.704904516184275	35.8	28.8	37.2	19.2	37.8
200-204	31.569370635358347	36.6	28.8	37.0	18.8	37.4
205-209	30.34757015535795	34.4	25.8	37.0	16.0	37.2
210-214	29.53063263851638	33.4	24.4	37.0	14.6	37.0
215-219	28.65693066688828	32.2	22.8	36.6	12.6	37.0
220-224	27.102957316228593	29.8	20.2	36.2	9.2	37.0
225-229	26.12381089092205	28.4	19.0	35.4	9.0	37.0
230-234	25.7960073838467	28.2	18.8	34.6	9.0	37.0
235-239	24.712140786686916	26.4	17.2	33.6	8.4	37.0
240-244	23.701121428013792	24.8	15.6	32.8	8.0	37.0
245-249	22.882287078520108	24.0	15.0	32.0	8.0	36.2
250-254	21.937241332416647	22.0	13.0	30.8	8.0	36.0
255-259	21.48626861386662	21.2	12.4	30.2	8.0	36.0
260-264	20.270665830569424	19.8	11.4	28.4	7.4	34.6
265-269	19.556490708491676	19.0	10.2	27.4	7.0	33.8
270-274	18.657018809669516	18.2	8.6	26.2	7.0	32.6
275-279	17.478971902599703	16.2	8.0	24.4	7.0	30.8
280-284	17.22710969746879	16.2	8.0	24.0	7.0	30.0
285-289	16.16385037888804	13.8	8.0	21.6	7.0	29.2
290-294	15.5818244548369	13.0	8.0	20.8	7.0	28.4
295-299	14.86388135477973	13.0	8.0	20.0	7.0	27.0
300-301	13.093088850441847	11.0	8.0	16.5	7.0	22.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.023115503977592766
1101	2	0.0732037285863143
1101	3	0.016939691155535286
1101	4	-0.02155367883167969
1101	5	0.014891854717802744
1101	6	0.14848873488822534
1101	7	0.0752678345181721
1101	8	0.10247838375933327
1101	9	0.007273883560912964
1101	10-14	0.05153750783503597
1101	15-19	0.03820708565034181
1101	20-24	0.058791153976358146
1101	25-29	0.11519245909702391
1101	30-34	0.16296254308504388
1101	35-39	0.17154294502689993
1101	40-44	0.09178919013433529
1101	45-49	0.12254127290897543
1101	50-54	0.14373386819430323
1101	55-59	0.18493765605418844
1101	60-64	0.3338018097398958
1101	65-69	0.33681481043824846
1101	70-74	0.21401405097439863
1101	75-79	0.10432034115807198
1101	80-84	0.19318837724084403
1101	85-89	0.08995568974607693
1101	90-94	0.20961252093020732
1101	95-99	0.061648151051869604
1101	100-104	0.1410688660331374
1101	105-109	0.20361157633178095
1101	110-114	0.2016838415130664
1101	115-119	0.2056248029068044
1101	120-124	0.1214193110842885
1101	125-129	0.17384202691998496
1101	130-134	0.10376899824450447
1101	135-139	0.12876589577148678
1101	140-144	0.11951152828775236
1101	145-149	0.461351393288048
1101	150-154	0.6578397609944204
1101	155-159	0.25191234035344934
1101	160-164	0.34695986914323385
1101	165-169	0.293682023668957
1101	170-174	0.21210626652206344
1101	175-179	0.26560686284393853
1101	180-184	0.061604864744623455
1101	185-189	0.14650421946438996
1101	190-194	0.4577650156367419
1101	195-199	0.24861462064527373
1101	200-204	-0.27929707494795153
1101	205-209	-0.43901640870994996
1101	210-214	-0.34479405916658834
1101	215-219	-0.5207518273997422
1101	220-224	0.25801177805874076
1101	225-229	0.5758553821079886
1101	230-234	0.06342961628763888
1101	235-239	0.4639138661696407
1101	240-244	0.41110013902967424
1101	245-249	0.2955715035301054
1101	250-254	0.13541519764232035
1101	255-259	0.10611787817660812
1101	260-264	0.4384548215882482
1101	265-269	0.18846867063217232
1101	270-274	-0.08963176978186382
1101	275-279	0.47257561403456094
1101	280-284	0.21924232329770277
1101	285-289	0.1895073284487232
1101	290-294	0.2718830443608091
1101	295-299	0.21533761259238737
1101	300-301	0.13979381586413986
1102	1	-0.08999443201158641
1102	2	-0.0768167414499743
1102	3	-0.026576655367136937
1102	4	-0.03095237612594559
1102	5	-0.01799390403821377
1102	6	0.053650687914100104
1102	7	0.15411988963604983
1102	8	-0.006339222684850654
1102	9	0.00859429310916937
1102	10-14	0.04545440317598093
1102	15-19	0.04732491929349436
1102	20-24	0.035868770452701426
1102	25-29	0.0034498504398570162
1102	30-34	0.058187861016051556
1102	35-39	0.007061224216066364
1102	40-44	-0.05157501422669952
1102	45-49	0.10423128648190527
1102	50-54	0.08740456985551504
1102	55-59	-0.035994523580512805
1102	60-64	0.1394703500603356
1102	65-69	0.1796735339931672
1102	70-74	0.18084355585770595
1102	75-79	-0.1384343724827204
1102	80-84	-0.009750824907222011
1102	85-89	0.079551563281818
1102	90-94	0.10196207538420055
1102	95-99	-0.018580560110386557
1102	100-104	0.028201143038828036
1102	105-109	-0.05792780728039304
1102	110-114	0.13782876199343264
1102	115-119	-0.014776815516952979
1102	120-124	0.13990320061247985
1102	125-129	0.019177071622117126
1102	130-134	0.11347161103255843
1102	135-139	0.11560163387304812
1102	140-144	0.13818665590275003
1102	145-149	0.2723777350897052
1102	150-154	0.8230545304022243
1102	155-159	0.4363110376477124
1102	160-164	0.5117622871893275
1102	165-169	0.4063970512574073
1102	170-174	0.4165563786462201
1102	175-179	0.4391951679987045
1102	180-184	0.4020654590026851
1102	185-189	0.7057795432966714
1102	190-194	0.7443628587219315
1102	195-199	0.5271387863529888
1102	200-204	-0.15248815074979305
1102	205-209	-0.08316548221028697
1102	210-214	-0.4503639376003896
1102	215-219	-0.6672769004279786
1102	220-224	0.5051428379271492
1102	225-229	1.0057145261285179
1102	230-234	0.7869513477207626
1102	235-239	0.7016397742278322
1102	240-244	0.9032199127159011
1102	245-249	0.9485278562491182
1102	250-254	0.3274593612845891
1102	255-259	0.20404671621620452
1102	260-264	0.9625493452080889
1102	265-269	1.1939485546721862
1102	270-274	0.8217528898622888
1102	275-279	0.9382270775499464
1102	280-284	0.32411603608280615
1102	285-289	0.9433721081837554
1102	290-294	0.5545731390762185
1102	295-299	0.7597149915425483
1102	300-301	0.36548887502438987
1103	1	-0.01907786524167676
1103	2	0.005755304456855015
1103	3	0.03611737197114451
1103	4	0.0335638407254919
1103	5	0.06393747165135011
1103	6	0.07929110857853061
1103	7	0.1747093606838348
1103	8	0.1061691827087543
1103	9	0.010839361264316949
1103	10-14	0.07665576872916091
1103	15-19	0.0735579145356482
1103	20-24	0.007558769554307787
1103	25-29	0.005134105288668422
1103	30-34	0.013226423736853121
1103	35-39	-0.5986891993431698
1103	40-44	-0.42219366027092065
1103	45-49	-0.19995530282035645
1103	50-54	-0.05695857581710584
1103	55-59	-0.363056850992038
1103	60-64	0.12466959759044016
1103	65-69	0.19945354635283508
1103	70-74	0.05021688782378675
1103	75-79	0.14927501369895424
1103	80-84	0.1949467578785402
1103	85-89	0.09437917671267115
1103	90-94	0.2534235929740447
1103	95-99	0.2206516882881715
1103	100-104	0.18641891827973467
1103	105-109	0.05993500399141283
1103	110-114	0.286706364035588
1103	115-119	-0.03105637278299156
1103	120-124	0.2875243267582732
1103	125-129	0.21029462371740948
1103	130-134	0.3655680818108351
1103	135-139	0.4508803299548845
1103	140-144	0.3381171440916404
1103	145-149	0.6643430408917652
1103	150-154	0.7681882952653751
1103	155-159	-0.13817722021024537
1103	160-164	-0.6453300500863293
1103	165-169	0.3210274725661364
1103	170-174	0.6977541597130639
1103	175-179	0.4844493534536767
1103	180-184	0.42492068840527253
1103	185-189	0.616678468948983
1103	190-194	0.3924980637318676
1103	195-199	0.3134424186627349
1103	200-204	0.16020682942664166
1103	205-209	0.923164992911115
1103	210-214	0.8236587000189317
1103	215-219	0.601155208946949
1103	220-224	1.4351477110136415
1103	225-229	1.9976469817013118
1103	230-234	0.6899885419844942
1103	235-239	1.0434602032590838
1103	240-244	1.5435705208535033
1103	245-249	1.2520594373887306
1103	250-254	1.0613367500724955
1103	255-259	0.7991300714043206
1103	260-264	1.890581752594489
1103	265-269	0.8037248555004268
1103	270-274	0.9227580887286848
1103	275-279	1.5925290510138552
1103	280-284	0.9568972980216444
1103	285-289	0.8484935678228922
1103	290-294	1.3684898392434661
1103	295-299	1.032116715362866
1103	300-301	-0.8389448169693203
2116	1	-0.011328294273717177
2116	2	-0.008572905273652509
2116	3	-0.008965730583220477
2116	4	0.060708580513910704
2116	5	0.05640473998068529
2116	6	-0.22345991831043222
2116	7	-0.19522556097522425
2116	8	0.04483463861559045
2116	9	0.1533284462821385
2116	10-14	0.14681399061151268
2116	15-19	0.10869508530500838
2116	20-24	-0.164977515625651
2116	25-29	-0.21330997982208544
2116	30-34	-0.08463253159337825
2116	35-39	-0.23804438122708405
2116	40-44	-0.24642036265854728
2116	45-49	-0.057620926003224326
2116	50-54	0.037182780393209214
2116	55-59	0.055950131233331035
2116	60-64	0.25204082381223714
2116	65-69	0.13231036426713416
2116	70-74	-0.9123440816337336
2116	75-79	0.13184343534783238
2116	80-84	-0.20408888253641777
2116	85-89	0.0023159375229937496
2116	90-94	0.21185665025767264
2116	95-99	0.1496861161731715
2116	100-104	0.12211004707431528
2116	105-109	0.21344872991893027
2116	110-114	0.36988184721106876
2116	115-119	0.34249295019162673
2116	120-124	-0.22022037638873826
2116	125-129	-0.18801155160026184
2116	130-134	0.09899098304982346
2116	135-139	0.3370064206786765
2116	140-144	0.38345815265103766
2116	145-149	0.6678409685276208
2116	150-154	1.018589890494546
2116	155-159	-0.4078665381754263
2116	160-164	-0.6168756009422438
2116	165-169	0.6201540084742803
2116	170-174	0.7910403725395057
2116	175-179	0.5047078081948868
2116	180-184	0.5329464860862387
2116	185-189	1.0116686846288587
2116	190-194	0.7657195610912915
2116	195-199	0.8732120910760806
2116	200-204	0.5654846094170658
2116	205-209	0.4616672440570433
2116	210-214	0.5217134302575666
2116	215-219	1.0671695876050045
2116	220-224	1.40285281664978
2116	225-229	1.0178895053921089
2116	230-234	0.9776638388551895
2116	235-239	1.1370852164243246
2116	240-244	1.6046299888095241
2116	245-249	0.6299251899671283
2116	250-254	0.7824102550540495
2116	255-259	0.6439087138841124
2116	260-264	0.20264695661371235
2116	265-269	0.47384324975675085
2116	270-274	0.7743488483654168
2116	275-279	1.2859977628733716
2116	280-284	0.21264429169966803
2116	285-289	-1.1205801920554652
2116	290-294	0.16409145323588348
2116	295-299	0.6051475711523437
2116	300-301	1.4699463022666261
1117	1	-0.024048057389926214
1117	2	0.06541623182997824
1117	3	0.04028506208653937
1117	4	0.03112973234959071
1117	5	0.025854538165020813
1117	6	-0.33971604122687893
1117	7	-0.2666756248134945
1117	8	-0.03944813643788336
1117	9	0.0928994061512256
1117	10-14	0.1500565949958954
1117	15-19	0.10744886115995911
1117	20-24	0.10606611577319569
1117	25-29	0.20115334050866096
1117	30-34	0.10448234385198418
1117	35-39	-0.08721927277432684
1117	40-44	0.029983514443074455
1117	45-49	0.13841378249581027
1117	50-54	0.17526210248776408
1117	55-59	0.2445889982102898
1117	60-64	0.24338899942936365
1117	65-69	0.4172728158010983
1117	70-74	-0.0541355107705499
1117	75-79	0.374954369356324
1117	80-84	0.10874030469810947
1117	85-89	0.23228892937355994
1117	90-94	0.3469469138604637
1117	95-99	0.4994914388297502
1117	100-104	0.4369717579398298
1117	105-109	0.5044021293404555
1117	110-114	0.5184496201366002
1117	115-119	0.4456535994353743
1117	120-124	0.6391697219483987
1117	125-129	0.5453107579986138
1117	130-134	0.26743653709639403
1117	135-139	0.5307279521103254
1117	140-144	0.585061473505867
1117	145-149	0.9645444201311619
1117	150-154	1.153668949645926
1117	155-159	0.0684249329780755
1117	160-164	-0.5283949516623423
1117	165-169	0.6120058422544332
1117	170-174	0.8258354349907719
1117	175-179	0.9614097417759879
1117	180-184	0.793927616525032
1117	185-189	1.068321824631191
1117	190-194	1.2114537824677534
1117	195-199	0.994794665624088
1117	200-204	1.073502026917513
1117	205-209	1.6008502046164672
1117	210-214	1.0014672576168557
1117	215-219	0.48026506127770574
1117	220-224	1.2188706642565919
1117	225-229	0.9781102692284769
1117	230-234	1.3471096612664475
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2108	175-179	-0.6947370852328092
2108	180-184	-0.08971492339465215
2108	185-189	-0.7031093951922003
2108	190-194	-0.23599963661644452
2108	195-199	-1.6376285416365661
2108	200-204	-0.881717952780722
2108	205-209	0.08233952165386427
2108	210-214	0.4713445837091612
2108	215-219	-0.5029449983644021
2108	220-224	-0.39208101954479346
2108	225-229	0.15311020107125728
2108	230-234	-0.11683217063604445
2108	235-239	-0.39573437121856614
2108	240-244	-0.10985421106149573
2108	245-249	-0.26734798505198754
2108	250-254	-0.5630973273207722
2108	255-259	-0.8773766299418249
2108	260-264	0.15142803509871072
2108	265-269	-0.6125835782173539
2108	270-274	-0.5565708598226742
2108	275-279	-0.28941047175167256
2108	280-284	-0.3712478217053352
2108	285-289	0.11408566916244922
2108	290-294	-0.27416627403808036
2108	295-299	0.17274893932678026
2108	300-301	-0.3946362657543361
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	9.0
14	43.0
15	144.0
16	245.0
17	520.0
18	1335.0
19	1333.0
20	1403.0
21	1876.0
22	2684.0
23	3866.0
24	5382.0
25	7517.0
26	10150.0
27	13891.0
28	19251.0
29	26830.0
30	37838.0
31	50582.0
32	63811.0
33	68584.0
34	55765.0
35	22171.0
36	1851.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.68175167408325	18.53139016276194	14.132561706242775	30.654296456912032
2	50.10816378439772	20.852567347380774	14.96160752286046	14.077661345361046
3	49.96537247880166	19.31611275224575	16.799006756773775	13.919508012178813
4	50.424218614244374	19.125472508266533	16.48144090782028	13.96886796966881
5	33.89467693152313	19.463940788197934	32.83469702807725	13.806685252201682
6	32.24892528766026	20.299786190796382	33.0306258389304	14.420662682612956
7	16.15077955994087	51.36306515262552	18.031494674916832	14.45466061251678
8	31.789071806146325	18.57646889944923	34.280238640284274	15.354220654120171
9	15.035143786059841	17.51094859261162	35.61849789590589	31.835409725422643
10-14	27.669278211356318	36.08676271711456	20.95637436002045	15.287584711508678
15-19	23.0058199419265	35.23087112769874	19.089762090041628	22.673546840333128
20-24	26.009020784067815	29.405288063200892	24.68058315264063	19.90510800009066
25-29	22.380258031696144	24.693930488084355	33.87956674045476	19.046244739764735
30-34	21.09584142358147	27.61160764877872	29.047478738701983	22.24507218893783
35-39	21.312571930805397	28.339264083327663	27.543208850542584	22.804955135324352
40-44	23.38035121120773	27.519888788993736	26.03531251652677	23.06444748327176
45-49	22.812560598162097	28.074735005024138	25.330472470491056	23.78223192632271
50-54	23.360002820569	28.172044635504417	26.988060430690812	21.47989211323577
55-59	22.671783984708487	25.426220714560937	27.754398954374775	24.147596346355797
60-64	23.529891735480994	25.572386629495597	27.34707857047519	23.55064306454822
65-69	21.214909729200194	28.90690359446262	26.82033730983195	23.05784936650524
70-74	21.228962206893772	25.18536426893118	28.939541607170288	24.64613191700476
75-79	21.967850550136873	25.115706288106015	28.02351145730238	24.892931704454735
80-84	26.11207228715407	26.01587073735214	25.623761279128043	22.248295696365748
85-89	24.26449885792139	27.47334940050317	24.070282535389325	24.191869206186112
90-94	22.824296179891864	28.133261811762278	27.377097483397677	21.665344524948186
95-99	22.95298464049078	27.77348816242448	26.114439550421448	23.15908764666329
100-104	22.08621371350574	28.796951770788475	24.3011158876104	24.815718628095386
105-109	25.276025415341373	27.122944069627756	23.17303938974975	24.42799112528111
110-114	21.41532123007029	27.902831397969695	28.371247321089044	22.310600050870978
115-119	24.857926428479686	27.218742681001203	24.384725611522022	23.538605278997085
120-124	20.47672652820695	26.218145828454	28.724573955571003	24.58055368776805
125-129	23.362067880015008	24.642001798112737	28.895218380036415	23.10071194183584
130-134	25.20853322856934	26.24086148236011	26.141587527040944	22.409017762029602
135-139	24.110727480148984	26.527451439623455	24.03416917873593	25.327651901491627
140-144	22.80571064487777	25.80659459105527	26.299035717973318	25.088659046093635
145-149	23.139444398274417	25.55450623673136	28.0334841834075	23.27256518158672
150-154	23.48758320048957	25.605377213328197	25.22052064681691	25.68651893936532
155-159	22.885442086415182	25.813041605911106	28.772976934293332	22.528539373380376
160-164	25.98862202612552	27.29061682318306	25.466010884374302	21.254750266317117
165-169	22.215204377926025	25.037586600282562	27.118159175789447	25.629049846001966
170-174	23.81451736790545	27.89547777164976	25.623056136878187	22.66694872356661
175-179	23.10700785478099	27.779078933119777	25.75547177794063	23.358441434158603
180-184	21.568034894467907	27.5994187613169	27.19592629248797	23.63662005172722
185-189	22.714999131164014	25.780957633542613	27.149034332872475	24.355008902420906
190-194	21.85054510014279	26.55485125276076	25.819387886159824	25.775215760936632
195-199	21.30718262932435	29.807470982137236	27.539985343114665	21.345361045423754
200-204	24.487827481911843	27.434717678671717	24.19181883888255	23.885636000533893
205-209	22.85809264058144	26.933764477451817	27.86974007952997	22.33840280243677
210-214	24.336725571228182	26.598066399216286	25.903199079285695	23.162008950269843
215-219	23.598139431806448	25.842959784226473	26.299791227526736	24.259109556440343
220-224	19.829154106320342	26.91613592120539	27.54225187177492	25.712458100699347
225-229	21.78521870742389	28.26517377978911	24.85394741149835	25.095660101288647
230-234	22.02929866048156	25.522422264362866	27.048803398785644	25.399475676369924
235-239	22.460845717394097	26.269268641568637	25.170808118201986	26.09907752283528
240-244	22.939234366618567	29.967689374765477	23.668502554881474	23.424573703734485
245-249	22.435057657970752	27.97123019620583	23.250907241055398	26.34280490476802
250-254	22.177982940594283	26.28201156936963	25.726661680303614	25.813343809732476
255-259	22.50113956024307	26.700563861963367	26.67799930996794	24.12029726782562
260-264	22.864086349705225	28.479637758352787	24.900436432685357	23.755839459256627
265-269	21.448311813902887	28.210021582389576	24.680784621854876	25.66088198185266
270-274	19.623806609701248	30.132088253589302	24.62769748390135	25.616407652808103
275-279	21.498125077124932	29.043147150595722	23.300821238884566	26.15790653339478
280-284	19.284330983698624	29.708549597943	25.31072848749506	25.696390930863323
285-289	20.25410304646636	27.695267739993906	26.914524167491432	25.136105046048307
290-294	21.28955407307792	30.69529544201086	24.080910036440745	23.93424044847047
295-299	20.228365354346572	31.147744929901304	22.57794969817393	26.04594001757819
300-301	16.39732751087304	33.80842292417454	24.928289551554712	24.865960013397704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.75
10	1.5
11	1.5
12	1.75
13	2.5
14	3.5
15	3.0
16	8.5
17	9.0
18	9.0
19	9.0
20	8.5
21	7.25
22	5.0
23	4.5
24	4.25
25	6.0
26	4.25
27	5.0
28	4.75
29	6.0
30	9.0
31	9.5
32	11.75
33	15.75
34	33.0
35	79.5
36	196.0
37	605.5
38	1992.5
39	3839.25
40	6017.25
41	8112.75
42	10467.75
43	14795.0
44	15366.5
45	12318.75
46	9760.75
47	7054.5
48	5779.25
49	4151.5
50	3252.25
51	2693.25
52	1575.5
53	1409.0
54	1687.75
55	2005.5
56	1991.25
57	2240.25
58	2978.5
59	3585.5
60	2872.0
61	2458.75
62	1314.25
63	437.75
64	315.25
65	349.75
66	253.0
67	133.5
68	67.75
69	31.75
70	13.5
71	7.5
72	4.75
73	3.25
74	2.5
75	1.25
76	1.25
77	0.25
78	0.75
79	1.0
80	1.0
81	1.0
82	0.25
83	0.0
84	0.25
85	0.0
86	0.25
87	0.25
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	397083.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.015161698208919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.64613169235296	6.537530782642744
2	10.563770479655197	2.9610590283026714
3	6.262955711967442	2.633290110358346
4	4.6653366183526055	2.6154178833114785
5	4.579475333316363	3.2091043644694013
6	3.4596028633734384	2.909213612505919
7	3.318954993587659	3.2560983632946296
8	2.702574347471023	3.0301613184990273
9	2.4703716924675136	3.1160392852149212
>10	14.835951219305993	35.155690108511415
>50	0.34262252111673525	3.135529819797246
>100	0.07811657662576772	2.484694092879194
>500	0.016273745215742253	1.5762804902678693
>1k	0.04520484780586524	13.941466010834791
>5k	0.010849163473407659	10.417135983156252
>10k+	0.0018081939122346097	3.0212887459540902
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	11922	3.0023949652843362	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	8451	2.1282704119793596	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	8361	2.1056051253768104	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	7264	1.8293404653435177	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	6229	1.5686896694142032	No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	5757	1.4498228330097234	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	5044	1.2702633958139733	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4464	1.1241982154864347	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3761	0.9471571434687458	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3608	0.9086261562444125	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3602	0.9071151371375757	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	3440	0.8663176212529874	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	3011	0.75827975511417	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC	2652	0.6678704452217799	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2292	0.5772092988115834	No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA	2262	0.5696542032774005	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	2213	0.5573142139049015	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2131	0.5366636194448012	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2066	0.5202942457874047	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1843	0.46413470231664417	No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	1807	0.45506858767562447	No Hit
GGGGGGAGCGAAAGCAGGCAGTATTCCGTATGGCATTTAAAATGCACAAT	1744	0.4392028870538401	No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTACCGAAAGGAAAACTCATCTTG	1737	0.4374400314291974	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	1669	0.42031514821838256	No Hit
GGGGGGAGCGAAAGCAGGGGTAGCAAACAGGATTAGATACCCTGGTAGTC	1617	0.40721964929246524	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT	1499	0.3775029401913454	TruSeq Adapter, Index 1 (97% over 36bp)
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1407	0.3543339805531841	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1359	0.34224582769849127	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGGCGTGCT	1310	0.3299058383259923	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	1265	0.31857319502471776	No Hit
GGGGGGAGCGAAAGCAGGCTTGACTGCGAGGCCTACAAGCCGAGCAGATG	1153	0.29036750503043446	No Hit
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG	1101	0.2772720061045172	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	887	0.22337899129401156	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	765	0.19265493612166726	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	755	0.19013657094360625	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	727	0.18308514844503543	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	683	0.17200434166156697	No Hit
GGGGGGAGCGAAAGCAGGGTCCGGTCGGTCTGCAGGCGGTCAGCCTCCTC	681	0.17150066862595478	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	617	0.15538313148636432	No Hit
CGGGTTATTAGTAGAAACAAGGGCTCCACGGTGACGACCACCAATTTCTC	570	0.14354681514947756	No Hit
GGGGGGAGCGAAAGCAGGCCCTGTCCCGTGGGGACTCCCTCCTCAAGCAG	535	0.13473253702626403	No Hit
GGGGGGAGCGAAAGCAGGAAGTATTTCGAAGAAAATGGAAAGAAATCAAA	459	0.11559296167300036	No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAACAACGCGAA	429	0.10803786613881733	No Hit
GGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGT	406	0.102245626229277	No Hit
GGGGGGAGCGAAAGCAGGTGACTTGATTGCCGTTTCAGGTATGGAAGACA	401	0.1009864436402465	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	4.533057320509818E-4	0.0	0.0
15-19	8.058768569795231E-4	0.0	0.0
20-24	0.001259182589030505	0.0	0.0
25-29	0.001259182589030505	0.0	0.0
30-34	0.0040293842848976155	0.0	0.0
35-39	0.0040293842848976155	0.0	0.0
40-44	0.004130118892020055	0.0	0.0
45-49	0.005490036088173001	0.0	0.0
50-54	0.0067492186772035055	0.0	0.0
55-59	0.008915012730335976	0.0	0.0
60-64	0.011836316336886747	0.0	0.0
65-69	0.015664231407539482	0.0	0.0
70-74	0.02080169637078394	0.0	0.0
75-79	0.02865899572633429	0.0	0.0
80-84	0.0419055965629352	0.0	0.0
85-89	0.05605880886363808	0.0	0.0
90-94	0.0750472823062181	0.0	0.0
95-99	0.09947542453340989	0.0	0.0
100-104	0.13100535656273374	0.0	0.0
105-109	0.16883120153721012	0.0	0.0
110-114	0.21778822059871614	0.0	0.0
115-119	0.28109992117516985	0.0	0.0
120-124	0.3535281036962045	0.0	0.0
125-129	0.4592490738712057	0.0	0.0
130-134	0.5817423561320934	0.0	0.0
135-139	0.7237781521747342	0.0	0.0
140-144	0.8870689503202103	0.0	0.0
145-149	1.0942296698675087	0.0	0.0
150-154	1.4143642513026244	0.0	0.0
155-159	1.7003498009232327	0.0	0.0
160-164	2.0131811233419716	0.0	0.0
165-169	2.370537142108829	0.0	0.0
170-174	2.78586592727465	0.0	0.0
175-179	3.2838978248879953	0.0	0.0
180-184	3.830836374259286	0.0	0.0
185-189	4.519407781244728	0.0	0.0
190-194	5.348101026737483	0.0	0.0
195-199	6.148135276503905	0.0	0.0
200-204	7.015057305399627	0.0	0.0
205-209	7.9832679817569625	0.0	0.0
210-214	9.071806146322054	0.0	0.0
215-219	10.119597162306118	0.0	0.0
220-224	11.301969613405763	0.0	0.0
225-229	12.519246605873331	0.0	0.0
230-234	13.751432320195022	0.0	0.0
235-239	14.968759679966155	0.0	0.0
240-244	16.18795062996905	0.0	0.0
245-249	17.33295054182627	0.0	0.0
250-254	18.531994570404674	0.0	0.0
255-259	19.66636698121048	0.0	0.0
260-264	20.75389779970434	0.0	0.0
265-269	21.839867231787814	0.0	0.0
270-274	23.194344759156145	0.0	0.0
275-279	24.055424180838767	0.0	0.0
280-284	24.841254851000922	0.0	0.0
285-289	25.54785775266128	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	6375	0.0	292.22354	1
GGGTTAT	6445	0.0	289.27853	2
GTTATTA	6505	0.0	286.6103	4
GGTTATT	6615	0.0	281.84427	3
GGGGGGA	6575	0.0	280.86694	1
TTAGTAG	6675	0.0	278.64795	8
TAGTAGA	6705	0.0	277.4012	9
TTATTAG	6735	0.0	276.60355	5
TATTAGT	6900	0.0	269.34784	6
GGGGAGC	6970	0.0	264.73816	3
GGGAGCG	5485	0.0	262.9991	4
ATTAGTA	7110	0.0	261.59988	7
GGAGCGA	5585	0.0	258.55417	5
CGAAAGC	5605	0.0	256.57895	9
GGGGGAG	7415	0.0	250.04382	2
GCGAAAG	5795	0.0	247.91199	8
AGCGAAA	5985	0.0	240.53467	7
GAGCGAA	6325	0.0	228.07115	6
GGGAGCA	1820	0.0	217.1978	4
GCAAAAG	2350	0.0	161.3085	8
>>END_MODULE