FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_6929_flu.3510000003bb85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_6929_flu.3510000003bb85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397083
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC119223.0023949652843362No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT84512.1282704119793596No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT83612.1056051253768104No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG72641.8293404653435177No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG62291.5686896694142032No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA57571.4498228330097234No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA50441.2702633958139733No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA44641.1241982154864347No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT37610.9471571434687458No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA36080.9086261562444125No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT36020.9071151371375757No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC34400.8663176212529874No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG30110.75827975511417No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC26520.6678704452217799No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT22920.5772092988115834No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA22620.5696542032774005No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA22130.5573142139049015No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG21310.5366636194448012No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA20660.5202942457874047No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT18430.46413470231664417No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA18070.45506858767562447No Hit
GGGGGGAGCGAAAGCAGGCAGTATTCCGTATGGCATTTAAAATGCACAAT17440.4392028870538401No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTACCGAAAGGAAAACTCATCTTG17370.4374400314291974No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC16690.42031514821838256No Hit
GGGGGGAGCGAAAGCAGGGGTAGCAAACAGGATTAGATACCCTGGTAGTC16170.40721964929246524No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT14990.3775029401913454TruSeq Adapter, Index 1 (97% over 36bp)
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG14070.3543339805531841No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT13590.34224582769849127No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGGCGTGCT13100.3299058383259923No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG12650.31857319502471776No Hit
GGGGGGAGCGAAAGCAGGCTTGACTGCGAGGCCTACAAGCCGAGCAGATG11530.29036750503043446No Hit
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG11010.2772720061045172No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT8870.22337899129401156No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG7650.19265493612166726No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA7550.19013657094360625No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA7270.18308514844503543No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6830.17200434166156697No Hit
GGGGGGAGCGAAAGCAGGGTCCGGTCGGTCTGCAGGCGGTCAGCCTCCTC6810.17150066862595478No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT6170.15538313148636432No Hit
CGGGTTATTAGTAGAAACAAGGGCTCCACGGTGACGACCACCAATTTCTC5700.14354681514947756No Hit
GGGGGGAGCGAAAGCAGGCCCTGTCCCGTGGGGACTCCCTCCTCAAGCAG5350.13473253702626403No Hit
GGGGGGAGCGAAAGCAGGAAGTATTTCGAAGAAAATGGAAAGAAATCAAA4590.11559296167300036No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAACAACGCGAA4290.10803786613881733No Hit
GGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGT4060.102245626229277No Hit
GGGGGGAGCGAAAGCAGGTGACTTGATTGCCGTTTCAGGTATGGAAGACA4010.1009864436402465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA63750.0292.223541
GGGTTAT64450.0289.278532
GTTATTA65050.0286.61034
GGTTATT66150.0281.844273
GGGGGGA65750.0280.866941
TTAGTAG66750.0278.647958
TAGTAGA67050.0277.40129
TTATTAG67350.0276.603555
TATTAGT69000.0269.347846
GGGGAGC69700.0264.738163
GGGAGCG54850.0262.99914
ATTAGTA71100.0261.599887
GGAGCGA55850.0258.554175
CGAAAGC56050.0256.578959
GGGGGAG74150.0250.043822
GCGAAAG57950.0247.911998
AGCGAAA59850.0240.534677
GAGCGAA63250.0228.071156
GGGAGCA18200.0217.19784
GCAAAAG23500.0161.30858
Produced by FastQC (version 0.11.2)