##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_6777_flu.3510000003b9f1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	294389
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.77168304522248	34.0	34.0	34.0	34.0	34.0
2	33.84623746131819	34.0	34.0	34.0	34.0	34.0
3	33.86941427838676	34.0	34.0	34.0	34.0	34.0
4	33.88890209892353	34.0	34.0	34.0	34.0	34.0
5	33.88044050558954	34.0	34.0	34.0	34.0	34.0
6	37.65406995505946	38.0	38.0	38.0	38.0	38.0
7	37.663472480289684	38.0	38.0	38.0	38.0	38.0
8	37.65830245015948	38.0	38.0	38.0	38.0	38.0
9	37.56814622828978	38.0	38.0	38.0	37.0	38.0
10-14	37.66194389056657	38.0	38.0	38.0	37.8	38.0
15-19	37.63363237077472	38.0	38.0	38.0	37.4	38.0
20-24	37.627880117803315	38.0	38.0	38.0	37.2	38.0
25-29	37.53044237386587	38.0	38.0	38.0	37.0	38.0
30-34	37.45638118272082	38.0	38.0	38.0	37.0	38.0
35-39	37.361268933282155	38.0	38.0	38.0	37.0	38.0
40-44	37.36755857046288	38.0	38.0	38.0	37.0	38.0
45-49	37.334818896086475	38.0	38.0	38.0	37.0	38.0
50-54	37.29531877889459	38.0	38.0	38.0	37.0	38.0
55-59	37.257473614842944	38.0	38.0	38.0	37.0	38.0
60-64	37.08029308160292	38.0	38.0	38.0	36.6	38.0
65-69	37.021560588201325	38.0	38.0	38.0	36.4	38.0
70-74	37.101144404172715	38.0	38.0	38.0	37.0	38.0
75-79	37.038463393673	38.0	38.0	38.0	36.8	38.0
80-84	36.94982489155505	38.0	38.0	38.0	36.2	38.0
85-89	36.98627258491316	38.0	38.0	38.0	36.0	38.0
90-94	36.841283471868856	38.0	38.0	38.0	35.8	38.0
95-99	36.841880640920685	38.0	38.0	38.0	36.0	38.0
100-104	36.826080458169294	38.0	38.0	38.0	36.0	38.0
105-109	36.68023533488004	38.0	38.0	38.0	35.0	38.0
110-114	36.59619618939566	38.0	38.0	38.0	34.8	38.0
115-119	36.68703246384886	38.0	38.0	38.0	35.0	38.0
120-124	36.60759946873015	38.0	37.8	38.0	34.8	38.0
125-129	36.51835156884259	38.0	37.2	38.0	34.0	38.0
130-134	36.405736627387576	38.0	37.0	38.0	34.0	38.0
135-139	36.16789010458951	38.0	37.0	38.0	33.6	38.0
140-144	36.13357564311166	38.0	37.0	38.0	33.4	38.0
145-149	35.72333409196675	38.0	37.0	38.0	30.6	38.0
150-154	35.36595592905985	38.0	36.4	38.0	28.6	38.0
155-159	35.4614044682376	38.0	36.4	38.0	29.4	38.0
160-164	35.42646022779384	38.0	36.0	38.0	28.8	38.0
165-169	35.21715417355948	38.0	36.0	38.0	28.2	38.0
170-174	35.06606632720652	38.0	35.4	38.0	28.0	38.0
175-179	34.709934134767266	38.0	35.2	38.0	25.4	38.0
180-184	34.4241768544341	37.6	35.0	38.0	23.8	38.0
185-189	33.64592019402899	37.0	33.8	37.8	22.6	38.0
190-194	32.638532010367236	36.8	31.0	37.0	20.8	38.0
195-199	32.37807322963833	36.8	30.4	37.2	20.2	37.8
200-204	32.1683391702815	37.0	30.0	37.0	19.8	37.4
205-209	31.069978837524502	35.4	28.0	37.0	17.2	37.2
210-214	30.302679787627937	34.4	26.2	37.0	15.4	37.0
215-219	29.385066697464918	33.2	24.2	37.0	12.6	37.0
220-224	27.858242665316983	31.4	21.4	36.2	9.2	37.0
225-229	26.785138711025205	29.8	20.0	36.0	9.0	37.0
230-234	26.30471926600518	29.0	19.2	35.6	9.0	37.0
235-239	25.250170352832477	27.4	17.6	34.6	8.8	37.0
240-244	24.12161052213228	25.8	15.6	33.2	8.0	37.0
245-249	23.194031026974514	24.2	14.0	32.2	8.0	36.2
250-254	22.238555788429597	22.6	13.0	31.2	8.0	36.0
255-259	21.57097853520342	21.4	12.0	30.4	8.0	36.0
260-264	20.257394128177342	19.8	10.6	29.2	7.4	34.8
265-269	19.429129485137015	18.6	8.8	27.6	7.0	33.8
270-274	18.4288509421208	17.6	8.0	26.2	7.0	33.0
275-279	17.179397328025164	15.8	8.0	24.0	7.0	31.0
280-284	16.896353464293846	15.0	8.0	23.8	7.0	30.4
285-289	15.821565343813798	13.4	8.0	21.4	7.0	29.0
290-294	15.162082822388067	12.8	8.0	20.6	7.0	28.0
295-299	14.376711765724943	12.0	8.0	19.0	7.0	26.4
300-301	12.779302215775726	10.0	8.0	16.0	7.0	22.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.028800735124619337
1101	2	0.03002141272488501
1101	3	0.0695605814599034
1101	4	0.056680124492700656
1101	5	0.036124294295568404
1101	6	0.07811581707973403
1101	7	0.04992154695621309
1101	8	0.06664334304560526
1101	9	0.02186569736901589
1101	10-14	0.06192552776900584
1101	15-19	0.08391475443928442
1101	20-24	0.04042615126397919
1101	25-29	0.048628455870066034
1101	30-34	0.050974212786456974
1101	35-39	0.11934471282045678
1101	40-44	0.12957093880507387
1101	45-49	0.16794526093310935
1101	50-54	0.09819546162302828
1101	55-59	0.10633075617712962
1101	60-64	0.2873381099681396
1101	65-69	0.2500938571219322
1101	70-74	0.249929754668905
1101	75-79	0.2037692618827407
1101	80-84	0.22564847527781495
1101	85-89	0.1945015464673574
1101	90-94	0.3447597191775458
1101	95-99	0.18833009515961407
1101	100-104	0.10591587598286623
1101	105-109	0.16997304216409503
1101	110-114	0.3380114029912775
1101	115-119	0.2900910178648033
1101	120-124	0.23516923560865166
1101	125-129	0.1290220401673139
1101	130-134	0.17041126287922737
1101	135-139	0.2408847037177324
1101	140-144	0.20095991461379015
1101	145-149	0.5647178833430644
1101	150-154	0.7531034777696135
1101	155-159	0.3985695663576152
1101	160-164	0.47196877938172577
1101	165-169	0.36596310406538635
1101	170-174	0.33899415021074475
1101	175-179	0.3925178677958385
1101	180-184	0.1858293737125507
1101	185-189	0.3163406905292092
1101	190-194	0.5766690417351796
1101	195-199	0.2492513262617848
1101	200-204	-0.0036019594486802475
1101	205-209	0.16577352329267114
1101	210-214	0.14896192126738583
1101	215-219	0.0036199049481311363
1101	220-224	0.6094465583326389
1101	225-229	0.9479994721956508
1101	230-234	0.22507511766172783
1101	235-239	0.5254501052164997
1101	240-244	0.2003532568596249
1101	245-249	0.3462050652822555
1101	250-254	0.4644213876479917
1101	255-259	-0.16396444481283368
1101	260-264	0.45780303088002583
1101	265-269	0.3465813622575631
1101	270-274	0.11008263917685923
1101	275-279	0.5618162782548914
1101	280-284	0.16477503826445172
1101	285-289	0.32188809040790467
1101	290-294	0.12199393587657426
1101	295-299	0.5119133072213167
1101	300-301	0.4244220408863626
1102	1	-0.14226149423144108
1102	2	-0.11948130786188926
1102	3	-0.020507782002518127
1102	4	-0.024415949757063515
1102	5	-0.012999072189728622
1102	6	0.06859542430539989
1102	7	0.08860437105150964
1102	8	-0.1440543097424154
1102	9	0.06690652277976739
1102	10-14	0.05496494471969271
1102	15-19	0.0244717605315401
1102	20-24	0.05418388284604703
1102	25-29	0.03565069226547024
1102	30-34	0.06779733810618893
1102	35-39	0.029898862130515624
1102	40-44	-0.05812230922341399
1102	45-49	0.10936195915724767
1102	50-54	0.10222485326130482
1102	55-59	0.057892353051940404
1102	60-64	0.16687225515910598
1102	65-69	0.21591146154396768
1102	70-74	0.0710686142148731
1102	75-79	-0.03583703366798119
1102	80-84	0.12159848823299768
1102	85-89	0.20925516561178625
1102	90-94	0.249691322496723
1102	95-99	0.03569554403981812
1102	100-104	0.2276739264615486
1102	105-109	0.11556197113651479
1102	110-114	0.22697957312262673
1102	115-119	0.018218363914137115
1102	120-124	0.15843872318164642
1102	125-129	0.07150438458894115
1102	130-134	0.30746837859219056
1102	135-139	0.3332796837534815
1102	140-144	0.14112295050958323
1102	145-149	0.38041224750971026
1102	150-154	0.8599916447119895
1102	155-159	0.583550200031766
1102	160-164	0.4707955055757651
1102	165-169	0.6643254370598868
1102	170-174	0.5116122250052868
1102	175-179	0.6759910505833986
1102	180-184	0.5808592370520671
1102	185-189	0.741285049713106
1102	190-194	0.7390777741875638
1102	195-199	0.7444512677974018
1102	200-204	0.4623644633949482
1102	205-209	0.5024047791474118
1102	210-214	0.27014330036238476
1102	215-219	0.3581797943973051
1102	220-224	1.1986791469094946
1102	225-229	1.2588339847820968
1102	230-234	1.0009671977313488
1102	235-239	1.2782201873988193
1102	240-244	1.3577034917136963
1102	245-249	1.463129838898876
1102	250-254	1.06447387271632
1102	255-259	0.8236078882481088
1102	260-264	1.4327238050015687
1102	265-269	1.2309474555746895
1102	270-274	0.88603185489049
1102	275-279	1.0968437498697696
1102	280-284	0.4704573773711438
1102	285-289	0.9703357946844395
1102	290-294	0.7405444178755225
1102	295-299	0.8069983131009728
1102	300-301	0.4004250704042285
1103	1	0.011126926150765826
1103	2	0.06019017058513754
1103	3	0.05787622007824922
1103	4	0.10244265459321156
1103	5	0.06135510552269352
1103	6	0.07582476582842901
1103	7	0.07571293455333716
1103	8	0.06944135031847054
1103	9	0.13518011734308288
1103	10-14	0.028171030971606115
1103	15-19	-0.014169536748454448
1103	20-24	0.08742584904020845
1103	25-29	0.1108875298953933
1103	30-34	0.08330240831548963
1103	35-39	-0.5485318238899808
1103	40-44	-0.22563462211251561
1103	45-49	-0.12827596687537834
1103	50-54	0.019795687803409123
1103	55-59	-0.268733831969854
1103	60-64	0.22130806307470152
1103	65-69	0.27842392448859243
1103	70-74	0.16519010583344595
1103	75-79	0.36123955133513874
1103	80-84	0.1713632345224525
1103	85-89	0.22888876337628972
1103	90-94	0.3239891460247577
1103	95-99	0.31694093816898317
1103	100-104	0.36466427955885194
1103	105-109	0.07410863340155771
1103	110-114	0.24935551888922447
1103	115-119	0.09821596302580815
1103	120-124	0.18832844904688528
1103	125-129	0.25793803170504503
1103	130-134	0.33909307973161873
1103	135-139	0.3215950270971888
1103	140-144	0.3958318594725654
1103	145-149	0.7359481291192509
1103	150-154	0.7021602470948736
1103	155-159	-0.014145280629421109
1103	160-164	-0.6483055618191642
1103	165-169	0.32632304284069136
1103	170-174	0.5921280158478908
1103	175-179	0.32311948810527724
1103	180-184	0.42100042262406134
1103	185-189	0.5837814400441914
1103	190-194	0.057896070290446744
1103	195-199	0.3625316241959382
1103	200-204	0.4485813636480387
1103	205-209	0.6301910600713541
1103	210-214	0.4565145910732795
1103	215-219	0.43291611350204207
1103	220-224	0.7936519535146083
1103	225-229	1.5340338087169556
1103	230-234	0.8315885276234098
1103	235-239	1.020690568106346
1103	240-244	1.1891904752309337
1103	245-249	1.1731458781667392
1103	250-254	1.3676347088923748
1103	255-259	0.30593601632216405
1103	260-264	1.5280564113992696
1103	265-269	0.6007622870623344
1103	270-274	0.7494779966295972
1103	275-279	1.474197704166194
1103	280-284	0.6950724849609529
1103	285-289	0.5886015530150228
1103	290-294	0.6962103476028574
1103	295-299	0.9528012601913947
1103	300-301	-0.7854211927131232
2116	1	-0.04260850004294525
2116	2	-0.011766324285702012
2116	3	0.06498535368348968
2116	4	0.06878880051666414
2116	5	0.049162133908140504
2116	6	-0.1540273332116584
2116	7	-0.0687273900872114
2116	8	0.0705569932138701
2116	9	0.1745440196213366
2116	10-14	0.15477392307393956
2116	15-19	0.13122658239627327
2116	20-24	-0.09591432316759807
2116	25-29	-0.2148393271062119
2116	30-34	0.09168077110543038
2116	35-39	-0.0732663025287934
2116	40-44	-0.06190669396189463
2116	45-49	0.17794378346450657
2116	50-54	0.2763213747807143
2116	55-59	0.20584417651694764
2116	60-64	0.41950292979905157
2116	65-69	0.2839892851774266
2116	70-74	-0.6509143723933946
2116	75-79	0.33869038147562236
2116	80-84	-0.09665637574409658
2116	85-89	0.1242121267841938
2116	90-94	0.3023954410851104
2116	95-99	0.2602979191766863
2116	100-104	0.28587286523018207
2116	105-109	0.2715299657461401
2116	110-114	0.41207887405752075
2116	115-119	0.3255233415945966
2116	120-124	0.013560764788664414
2116	125-129	-0.10514852537483677
2116	130-134	0.1442850213951914
2116	135-139	0.29635051673587753
2116	140-144	0.5635543494820396
2116	145-149	0.7647205099539107
2116	150-154	1.0725124903280943
2116	155-159	-0.3165758001366896
2116	160-164	-0.5274819306240133
2116	165-169	0.728363169730649
2116	170-174	0.7271412404184048
2116	175-179	0.7189143218711891
2116	180-184	0.638959801357629
2116	185-189	0.9931007660442788
2116	190-194	0.8011887823573574
2116	195-199	0.9370290493184967
2116	200-204	0.8404646908016673
2116	205-209	0.5613125530882819
2116	210-214	0.7879086503160586
2116	215-219	1.3710729132383683
2116	220-224	1.4699029319680328
2116	225-229	1.3896828014249003
2116	230-234	1.5851181284144111
2116	235-239	1.6115172479563853
2116	240-244	1.733968294206747
2116	245-249	1.183986235765719
2116	250-254	1.4378305643696514
2116	255-259	0.8998861666950084
2116	260-264	0.15607931750893655
2116	265-269	0.9896180527278915
2116	270-274	1.2301309031512488
2116	275-279	1.744844760566309
2116	280-284	0.2441091924136778
2116	285-289	-0.7932073704802214
2116	290-294	0.6138330559619387
2116	295-299	0.7186091949337019
2116	300-301	1.2941511716423335
1117	1	-0.06458358460692892
1117	2	0.04344364188870031
1117	3	0.03477992508849326
1117	4	-0.03823537240636199
1117	5	0.05233183937798458
1117	6	-0.4782250066709395
1117	7	-0.26219327574020923
1117	8	-0.07156494392003054
1117	9	0.06444914296099569
1117	10-14	0.06997365906931918
1117	15-19	0.07257630601643683
1117	20-24	0.03207280660716805
1117	25-29	0.1557023773736077
1117	30-34	0.13283777135292496
1117	35-39	-0.06999429526867118
1117	40-44	-0.015230347125829269
1117	45-49	0.1898698621708803
1117	50-54	0.16113318898745632
1117	55-59	0.27269806646002337
1117	60-64	0.33064326475491157
1117	65-69	0.3706095310317252
1117	70-74	-0.13805173729728182
1117	75-79	0.23488475487715732
1117	80-84	0.09810313254730119
1117	85-89	0.17237910970639803
1117	90-94	0.3587181867331637
1117	95-99	0.21126665112949183
1117	100-104	0.24163625108943165
1117	105-109	0.3057584947453975
1117	110-114	0.3135995406691805
1117	115-119	0.30537120893227154
1117	120-124	0.46300157000275277
1117	125-129	0.40343162477779515
1117	130-134	0.2034510968015084
1117	135-139	0.3526429018526116
1117	140-144	0.3537898086025777
1117	145-149	0.7516288508498832
1117	150-154	0.9531230010120311
1117	155-159	-0.16196875451338855
1117	160-164	-0.8199746292462322
1117	165-169	0.42267552019679044
1117	170-174	0.5837576912062943
1117	175-179	0.9430908749608662
1117	180-184	0.7880270400343079
1117	185-189	0.9395395087222695
1117	190-194	1.1477700875302048
1117	195-199	0.9713288595651832
1117	200-204	0.6704978563820632
1117	205-209	1.0284997488642063
1117	210-214	0.6257969433611841
1117	215-219	0.37717306999111955
1117	220-224	0.9185391831024283
1117	225-229	0.12040336903483961
1117	230-234	1.0461322361612986
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2108	170-174	-0.41924328854965864
2108	175-179	-0.6288333911764781
2108	180-184	-0.6565009593549362
2108	185-189	-0.7971377189501041
2108	190-194	-0.804431182335307
2108	195-199	-1.8512347927614208
2108	200-204	-1.0756880936631035
2108	205-209	-0.5889946207200936
2108	210-214	-0.046663007523775946
2108	215-219	-0.735476597273582
2108	220-224	-0.46841034037990426
2108	225-229	-0.27238524543755815
2108	230-234	-0.16298458383081282
2108	235-239	-0.37808185331147115
2108	240-244	-0.0804393450164902
2108	245-249	-0.15698888861239269
2108	250-254	-0.6364445118620914
2108	255-259	-0.7664425124897143
2108	260-264	0.01022792928874594
2108	265-269	-0.7421524495991818
2108	270-274	-0.6208618748940076
2108	275-279	-0.07893986469448322
2108	280-284	-0.53738591270589
2108	285-289	-0.24952963014961504
2108	290-294	-0.6616736042852125
2108	295-299	0.05234663675684459
2108	300-301	-0.19393180059326198
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	21.0
13	154.0
14	373.0
15	500.0
16	447.0
17	451.0
18	755.0
19	688.0
20	692.0
21	880.0
22	1442.0
23	2164.0
24	3309.0
25	4685.0
26	6831.0
27	9816.0
28	13594.0
29	18992.0
30	26295.0
31	35953.0
32	46900.0
33	53758.0
34	45716.0
35	18447.0
36	1520.0
37	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.651546083583284	24.137111101297943	15.887821895519194	31.32352091959958
2	41.09596486281756	24.99923570513844	18.636905590901833	15.267893841142163
3	40.139067696143535	23.84769811372028	20.493972261191825	15.519261928944356
4	40.84018084914858	23.439394814344286	20.084649902000415	15.635774434506725
5	27.49559256629833	23.47777940072489	33.660564762949704	15.366063270027071
6	26.16979574644433	23.469626922201577	34.0315025357605	16.329074795593584
7	18.06181616840303	44.67762042739369	21.287140484189287	15.973422920013993
8	25.689818573384194	22.270533206064087	35.11578217936132	16.923866041190397
9	16.43199983695043	20.751794394491643	36.70993141727442	26.106274351283503
10-14	25.536687851787942	33.670687423782816	24.03269143887849	16.75993328555075
15-19	21.50562690861411	34.44666750456029	22.696092584981095	21.3516130018445
20-24	24.719198067862592	30.61676896894925	26.635234332804554	18.02879863038361
25-29	19.780154829154622	26.175910105336815	36.04258311282011	18.00135195268845
30-34	20.066986198533233	28.765612845588663	30.38333633389835	20.784064621979763
35-39	21.093111495334405	29.819116882763964	27.90437142692152	21.183400194980113
40-44	20.755395072506104	29.297697943876976	28.5190003702584	21.427906613358516
45-49	21.126468719958964	30.43089245861768	26.578778419030602	21.863860402392753
50-54	22.096206040307212	29.482555394393135	27.77977438015687	20.641464185142787
55-59	21.529880532220975	27.312025924881706	29.340158769519242	21.81793477337808
60-64	22.098719721185233	26.478706745156916	28.339849654708566	23.08272387894928
65-69	20.719048605756328	27.34490758825907	29.201226948017762	22.734816857966837
70-74	21.274300330515068	26.500514625206783	29.305374861153098	22.91981018312505
75-79	20.749824212181842	26.137457581635182	30.312817394671676	22.7999008115113
80-84	23.021512352703397	26.250844970430283	27.60612658761027	23.12151608925605
85-89	21.20113183576832	28.325107256045573	26.627013916960212	23.846746991225896
90-94	20.729714765157667	29.90736746277884	28.627292459976427	20.73562531208707
95-99	22.047630855772464	29.935765262968385	26.865202164483048	21.151401716776103
100-104	22.544932045694644	28.754471124940128	26.686594947501437	22.01400188186379
105-109	23.44849841536199	27.798525080760488	24.59562008091335	24.157356422964174
110-114	21.587763129736505	28.101253783259565	29.03851706415661	21.272466022847322
115-119	22.806830418256116	28.337879472398765	27.188719687216572	21.666570422128544
120-124	18.9310062536304	29.189609666122035	27.96171052586883	23.91767355437873
125-129	20.944328762283916	26.080526106614037	29.831617349833046	23.143527781269
130-134	24.167818770402427	28.782936862450704	26.38474943017572	20.66449493697115
135-139	23.696333762470744	27.833173114484577	25.3683391702815	23.102153952763178
140-144	21.11097901076467	29.373380119501746	26.748349972315545	22.76729089741804
145-149	20.851866068365325	27.5102670276403	28.569545737102946	23.068321166891426
150-154	22.76022541603117	26.370958154007113	25.930452564464023	24.938363865497692
155-159	21.487555581220764	27.47453199677977	29.65701843479206	21.380893987207404
160-164	24.349075542904117	28.773969136075056	26.372860398995883	20.50409492202494
165-169	20.245729290156902	26.273264286369397	28.00994602379844	25.47106039967526
170-174	21.8514278726447	29.05706395279715	26.5708976897914	22.520610484766753
175-179	20.461769971024733	29.39050032440071	27.863880783589064	22.2838489209855
180-184	19.923366701880845	29.421343868147247	26.736732690419817	23.918556739552088
185-189	21.787023292310515	27.981955847535062	27.515022640112228	22.715998220042188
190-194	20.465370649039198	28.47450142498531	26.580884475982458	24.47924344999304
195-199	20.990594077903726	29.97224760436022	26.826613766139364	22.210544551596698
200-204	21.678867077234543	28.465669573251716	26.93259598694245	22.922867362571292
205-209	21.186457374426354	28.087598381733013	27.41318459589183	23.312759647948802
210-214	22.17331489967356	28.080668774988194	27.075604047705585	22.670412277632657
215-219	21.901769427526162	28.092421931525973	26.946726949716194	23.05908169123167
220-224	19.738169564759552	28.100778222012373	27.125266229376777	25.035785983851298
225-229	20.882437862827754	29.377184609479297	26.076585741994435	23.663791785698514
230-234	20.446619948435576	28.28271436772434	27.122209049930536	24.148456633909554
235-239	20.747446405945873	28.4710366216129	25.588320215768935	25.193196756672293
240-244	21.517448002472918	31.428416143266226	23.560798807020642	23.493337047240217
245-249	19.705491713345268	30.34685399250651	24.405327644714987	25.542326649433235
250-254	19.907333494118326	28.574505161537967	25.479416690161656	26.03874465418205
255-259	20.009579162264895	29.70905842269922	25.916525413653364	24.364837001382526
260-264	19.870579403442385	30.514591238123707	25.430637693663826	24.184191664770083
265-269	19.117562137172246	30.02836383152903	25.465489539350994	25.38858449194773
270-274	18.25713596635744	31.70308673218089	24.347377109878426	25.692400191583246
275-279	18.937867923054192	30.882471831488267	23.8137973905275	26.36586285493004
280-284	16.896079676890103	32.129053735024065	25.42771638885964	25.54715019922619
285-289	18.43553937137597	30.819697746858747	26.017480272700404	24.727282609064876
290-294	17.992927724881024	32.73172570986008	24.421428789798533	24.85391777546036
295-299	17.371437112120358	33.67856815302202	22.316187085794645	26.633807649062973
300-301	13.257288825329749	37.44891962675236	26.532581040731824	22.76121050718607
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.25
30	0.5
31	0.25
32	1.75
33	5.75
34	19.0
35	56.0
36	171.5
37	531.5
38	1680.25
39	2860.5
40	5014.25
41	6899.75
42	9794.0
43	13677.75
44	14146.75
45	11919.0
46	9213.25
47	6925.0
48	5692.5
49	3866.75
50	2669.5
51	1980.0
52	617.5
53	141.5
54	73.25
55	52.75
56	44.0
57	36.25
58	24.0
59	30.0
60	26.0
61	15.5
62	8.75
63	4.75
64	2.0
65	2.25
66	2.5
67	1.25
68	1.5
69	1.0
70	1.0
71	0.0
72	0.0
73	0.0
74	0.25
75	0.25
76	0.0
77	0.25
78	0.25
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.25
89	0.0
90	0.0
91	0.0
92	0.0
93	0.25
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	294389.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.068368516048125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	39.00181417020251	5.486918945408366
2	9.762994947960358	2.746988214964448
3	6.427620593587016	2.7127840557576643
4	5.698877281654719	3.2069562290421265
5	4.838432865492153	3.403442829595115
6	4.667375999924409	3.939741934193712
7	4.4070711148582316	4.3400210364179665
8	3.5591193798922394	4.005680242314616
9	2.8500952728368087	3.6086571543703454
>10	18.448226731106075	42.82931050845906
>50	0.2320717335350118	2.055282442970528
>100	0.048388352370101105	1.133244199935585
>500	0.016890870669427214	1.747237333797316
>1k	0.03619472286260368	13.371270903505328
>5k	0.004825963048347158	5.4124639692677885
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	8949	3.0398554293808533	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	6995	2.3761078029410068	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	4680	1.589733312046306	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	4660	1.5829395799435442	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4533	1.5397993810910056	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	4351	1.4779764189558713	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3153	1.071031866000428	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	2993	1.016682009178332	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	2495	0.8475180798195585	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2087	0.7089259449232138	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1943	0.6600110737833275	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1832	0.6223058606129985	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	1672	0.5679560037909025	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1420	0.48235497929610144	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1332	0.45246255804394864	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1164	0.39539520838074793	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1074	0.36482341391831896	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	923	0.31353073654246594	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	855	0.29043204739307515	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	806	0.2737874037413083	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	778	0.2642761787974415	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	646	0.21943754691921233	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT	629	0.21366287463186465	TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	510	0.1732401686204308	No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG	401	0.13621432866037794	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	6.793732102761992E-4	0.0	0.0
9	6.793732102761992E-4	0.0	0.0
10-14	0.0019022449887733579	0.0	0.0
15-19	0.002717492841104797	0.0	0.0
20-24	0.003328928730353376	0.0	0.0
25-29	0.004551800508850535	0.0	0.0
30-34	0.006114358892485792	0.0	0.0
35-39	0.006861669423789611	0.0	0.0
40-44	0.008220415844342011	0.0	0.0
45-49	0.010802034043391567	0.0	0.0
50-54	0.014606524020938283	0.0	0.0
55-59	0.020652945592396454	0.0	0.0
60-64	0.030096233215235624	0.0	0.0
65-69	0.04483863187822915	0.0	0.0
70-74	0.06848081959584087	0.0	0.0
75-79	0.10462347438253468	0.0	0.0
80-84	0.1500735421500124	0.0	0.0
85-89	0.21760323925146657	0.0	0.0
90-94	0.30279663982010196	0.0	0.0
95-99	0.40830329937599574	0.0	0.0
100-104	0.5500205510396109	0.0	0.0
105-109	0.7202714775348263	0.0	0.0
110-114	0.9272085573849566	0.0	0.0
115-119	1.1804109528548963	0.0	0.0
120-124	1.494145501360445	0.0	0.0
125-129	1.8591727272418468	0.0	0.0
130-134	2.2927487100401174	0.0	0.0
135-139	2.7827806066123397	0.0	0.0
140-144	3.3228143714608906	0.0	0.0
145-149	3.9392776224655135	0.0	0.0
150-154	4.663693276583025	0.0	0.0
155-159	5.483560866744342	0.0	0.0
160-164	6.376868700936516	0.0	0.0
165-169	7.376974003784109	0.0	0.0
170-174	8.512478387439748	0.0	0.0
175-179	9.761981595779734	0.0	0.0
180-184	11.126366813977427	0.0	0.0
185-189	12.654005414604487	0.0	0.0
190-194	14.308211244306003	0.0	0.0
195-199	15.901952858292939	0.0	0.0
200-204	17.513697862352196	0.0	0.0
205-209	19.229726654188845	0.0	0.0
210-214	21.039712761006694	0.0	0.0
215-219	22.979051527061138	0.0	0.0
220-224	24.83822425430298	0.0	0.0
225-229	26.68496445179677	0.0	0.0
230-234	28.549164540794663	0.0	0.0
235-239	30.363906260084445	0.0	0.0
240-244	32.045558767481126	0.0	0.0
245-249	33.62815866081952	0.0	0.0
250-254	35.28548960728831	0.0	0.0
255-259	36.83636277170682	0.0	0.0
260-264	38.30183872359361	0.0	0.0
265-269	39.62335549222287	0.0	0.0
270-274	40.79975814313714	0.0	0.0
275-279	41.86046353634138	0.0	0.0
280-284	42.81573020731073	0.0	0.0
285-289	43.67697162597788	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCGTT	10	8.4618333E-4	295.0	295
GGTCTGG	10	8.4618333E-4	295.0	295
CGGGTTA	3940	0.0	293.8769	1
GGGTTAT	3975	0.0	290.91824	2
GTTATTA	4010	0.0	287.6434	4
GGGGGGA	2760	0.0	283.7772	1
GGTTATT	4115	0.0	281.02066	3
TTATTAG	4165	0.0	276.58463	5
TTAGTAG	4175	0.0	275.92215	8
GGGGAGC	2855	0.0	273.81787	3
TAGTAGA	4255	0.0	270.73444	9
TATTAGT	4265	0.0	270.09964	6
GGGAGCG	1930	0.0	268.25128	4
GGAGCGA	1950	0.0	265.5	5
CGAAAGC	2095	0.0	247.82816	9
GCGAAAG	2095	0.0	247.82816	8
ATTAGTA	4665	0.0	246.93996	7
GAGCGAA	2155	0.0	240.2436	6
AGCGAAA	2160	0.0	239.68748	7
GGGGGAG	3615	0.0	216.65973	2
>>END_MODULE