FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_6777_flu.3510000003b9f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_6777_flu.3510000003b9f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294389
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC89493.0398554293808533No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT69952.3761078029410068No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG46801.589733312046306No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA46601.5829395799435442No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA45331.5397993810910056No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG43511.4779764189558713No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA31531.071031866000428No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT29931.016682009178332No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT24950.8475180798195585No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG20870.7089259449232138No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT19430.6600110737833275No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA18320.6223058606129985No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA16720.5679560037909025No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT14200.48235497929610144No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG13320.45246255804394864No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT11640.39539520838074793No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT10740.36482341391831896No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT9230.31353073654246594No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA8550.29043204739307515No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG8060.2737874037413083No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA7780.2642761787974415No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6460.21943754691921233No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT6290.21366287463186465TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG5100.1732401686204308No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG4010.13621432866037794No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT108.4618333E-4295.0295
GGTCTGG108.4618333E-4295.0295
CGGGTTA39400.0293.87691
GGGTTAT39750.0290.918242
GTTATTA40100.0287.64344
GGGGGGA27600.0283.77721
GGTTATT41150.0281.020663
TTATTAG41650.0276.584635
TTAGTAG41750.0275.922158
GGGGAGC28550.0273.817873
TAGTAGA42550.0270.734449
TATTAGT42650.0270.099646
GGGAGCG19300.0268.251284
GGAGCGA19500.0265.55
CGAAAGC20950.0247.828169
GCGAAAG20950.0247.828168
ATTAGTA46650.0246.939967
GAGCGAA21550.0240.24366
AGCGAAA21600.0239.687487
GGGGGAG36150.0216.659732
Produced by FastQC (version 0.11.2)