##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_6667_flu.3510000003ba36.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	348094
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.78561825254098	34.0	34.0	34.0	34.0	34.0
2	33.87744689652795	34.0	34.0	34.0	34.0	34.0
3	33.89636707326182	34.0	34.0	34.0	34.0	34.0
4	33.904451096542886	34.0	34.0	34.0	34.0	34.0
5	33.89265543215338	34.0	34.0	34.0	34.0	34.0
6	37.67012071451964	38.0	38.0	38.0	38.0	38.0
7	37.68357972271858	38.0	38.0	38.0	38.0	38.0
8	37.68486673140014	38.0	38.0	38.0	38.0	38.0
9	37.60804840071935	38.0	38.0	38.0	38.0	38.0
10-14	37.67804098892828	38.0	38.0	38.0	38.0	38.0
15-19	37.64324464081541	38.0	38.0	38.0	37.8	38.0
20-24	37.63834424034887	38.0	38.0	38.0	37.8	38.0
25-29	37.553840054697865	38.0	38.0	38.0	37.0	38.0
30-34	37.494087229311624	38.0	38.0	38.0	37.0	38.0
35-39	37.39675432498119	38.0	38.0	38.0	37.0	38.0
40-44	37.40276706866537	38.0	38.0	38.0	37.0	38.0
45-49	37.37591167902923	38.0	38.0	38.0	37.0	38.0
50-54	37.33897567898326	38.0	38.0	38.0	37.0	38.0
55-59	37.29659919447046	38.0	38.0	38.0	37.0	38.0
60-64	37.12168954362902	38.0	38.0	38.0	36.6	38.0
65-69	37.0559032904905	38.0	38.0	38.0	36.4	38.0
70-74	37.13906990640459	38.0	38.0	38.0	37.0	38.0
75-79	37.05578722988618	38.0	38.0	38.0	36.8	38.0
80-84	36.961439151493565	38.0	38.0	38.0	36.4	38.0
85-89	36.99872505702483	38.0	38.0	38.0	36.0	38.0
90-94	36.85365217441266	38.0	38.0	38.0	35.8	38.0
95-99	36.853087384442134	38.0	38.0	38.0	36.0	38.0
100-104	36.83607358931782	38.0	38.0	38.0	36.0	38.0
105-109	36.690063028951954	38.0	38.0	38.0	35.0	38.0
110-114	36.598972691284544	38.0	38.0	38.0	34.8	38.0
115-119	36.70260446890782	38.0	38.0	38.0	35.0	38.0
120-124	36.61587272403431	38.0	37.8	38.0	34.8	38.0
125-129	36.52161312748855	38.0	37.4	38.0	34.0	38.0
130-134	36.39097140427585	38.0	37.0	38.0	34.0	38.0
135-139	36.147691140898715	38.0	37.0	38.0	33.6	38.0
140-144	36.1213540020799	38.0	37.0	38.0	33.4	38.0
145-149	35.7105471510569	38.0	37.0	38.0	30.6	38.0
150-154	35.35041511775555	38.0	36.4	38.0	28.4	38.0
155-159	35.475456054973655	38.0	36.4	38.0	29.4	38.0
160-164	35.40297505846122	38.0	36.0	38.0	28.6	38.0
165-169	35.21971823702793	38.0	36.0	38.0	28.2	38.0
170-174	35.09953575758272	38.0	35.6	38.0	28.0	38.0
175-179	34.754892643941005	38.0	35.2	38.0	25.6	38.0
180-184	34.49391313840514	37.6	35.0	38.0	24.4	38.0
185-189	33.74081253914172	37.0	33.8	37.8	22.8	38.0
190-194	32.758335392164184	36.8	31.6	37.0	21.2	38.0
195-199	32.52893069113515	36.8	30.6	37.2	20.6	37.8
200-204	32.33889121903853	37.0	30.2	37.0	20.0	37.4
205-209	31.251702126437113	35.4	28.4	37.0	18.2	37.2
210-214	30.553379259625274	34.6	27.0	37.0	16.6	37.0
215-219	29.683825633305947	33.4	24.8	37.0	14.6	37.0
220-224	28.22074209839871	31.6	22.4	36.2	9.6	37.0
225-229	27.209573850741464	30.2	20.2	36.2	9.2	37.0
230-234	26.80199313978408	29.6	20.0	36.0	9.0	37.0
235-239	25.677831850017526	28.2	18.4	34.6	8.8	37.0
240-244	24.59663883893431	26.6	16.6	33.8	8.2	37.0
245-249	23.699132992812288	25.0	15.6	33.0	8.0	36.4
250-254	22.800921015587743	23.8	14.0	31.8	8.0	36.0
255-259	22.18826351502755	22.4	13.2	31.0	8.0	36.0
260-264	20.88623015622217	20.6	11.8	29.4	7.8	35.2
265-269	20.114520790361226	19.6	10.4	28.6	7.0	34.2
270-274	19.03359782127816	18.4	8.8	26.8	7.0	33.2
275-279	17.699432911799683	16.4	8.0	25.0	7.0	31.2
280-284	17.454882876464403	16.4	8.0	24.4	7.0	30.8
285-289	16.361187495331723	14.6	8.0	22.6	7.0	29.4
290-294	15.601041098094191	13.0	8.0	21.0	7.0	28.4
295-299	14.813763523645912	13.0	8.0	20.0	7.0	27.0
300-301	13.08043660620407	10.5	8.0	16.5	7.0	22.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.04744473112626224
1101	2	0.016962994613443527
1101	3	0.023314145759258054
1101	4	-0.03182108520853433
1101	5	0.006286233142930087
1101	6	0.07390206334401483
1101	7	0.0987987435674782
1101	8	0.05413859700912127
1101	9	0.12209516990385794
1101	10-14	0.02691028530078654
1101	15-19	0.03879149732191678
1101	20-24	0.041330165841671374
1101	25-29	0.06971395032411465
1101	30-34	0.13531855887222832
1101	35-39	0.15345203396420004
1101	40-44	0.13938919384148818
1101	45-49	0.1392939501912096
1101	50-54	0.14453064171186725
1101	55-59	0.1640023597008593
1101	60-64	0.25686449900580044
1101	65-69	0.3014213018557541
1101	70-74	0.18801276445348947
1101	75-79	0.1898140793533969
1101	80-84	0.29408798794819546
1101	85-89	0.15883677226493376
1101	90-94	0.3749387899125267
1101	95-99	0.2486047766053261
1101	100-104	0.22989837884858844
1101	105-109	0.2564056575466651
1101	110-114	0.25072124494753467
1101	115-119	0.252939460773014
1101	120-124	0.2735120366070518
1101	125-129	0.2840003520067853
1101	130-134	0.27658819897592224
1101	135-139	0.17306434076871113
1101	140-144	0.3731958156151478
1101	145-149	0.5093185522935784
1101	150-154	0.8915742040743666
1101	155-159	0.3956193171510165
1101	160-164	0.3818080103589381
1101	165-169	0.5308905787311247
1101	170-174	0.6193228427144959
1101	175-179	0.47330693719315775
1101	180-184	0.4288756592842802
1101	185-189	0.5843512666640791
1101	190-194	0.6743773325643332
1101	195-199	0.47127430891748645
1101	200-204	0.18172326036482644
1101	205-209	0.05949959992657128
1101	210-214	-0.2503971051821914
1101	215-219	0.13101720800399264
1101	220-224	0.7869275712111907
1101	225-229	1.177864841136138
1101	230-234	0.6319791902173222
1101	235-239	0.6737105153254284
1101	240-244	0.9077432448062126
1101	245-249	0.640939511208451
1101	250-254	0.4699720707918722
1101	255-259	-0.14632109071726163
1101	260-264	0.7266565192100103
1101	265-269	0.2508511754207028
1101	270-274	0.29523273637155256
1101	275-279	0.31757223195177886
1101	280-284	0.0623774000142312
1101	285-289	0.0778013009923022
1101	290-294	0.27062920393759704
1101	295-299	0.15950583696471377
1101	300-301	-0.19728754411490712
1102	1	-0.04791424295434865
1102	2	-0.01936999762155267
1102	3	-0.013189796936217135
1102	4	0.009935370579107428
1102	5	-0.0043692776016683865
1102	6	0.08755161490002195
1102	7	0.12016995888078696
1102	8	-0.011731504718447638
1102	9	0.05659104662690595
1102	10-14	0.02053840785541894
1102	15-19	0.03455433365490279
1102	20-24	0.007543131591390306
1102	25-29	0.03804236782848136
1102	30-34	0.18702770231411847
1102	35-39	0.08241056041938322
1102	40-44	-0.037789160459361426
1102	45-49	0.08382510049518288
1102	50-54	0.08029973820256231
1102	55-59	-0.0014639618545473354
1102	60-64	0.16859386757258932
1102	65-69	0.15058039413289492
1102	70-74	0.0993214985773676
1102	75-79	-0.054043863221686195
1102	80-84	0.13486087664980317
1102	85-89	0.07246995110077847
1102	90-94	0.2468419982433474
1102	95-99	0.05092368348548604
1102	100-104	0.12951410147545062
1102	105-109	0.04637881110111408
1102	110-114	0.11326527672987652
1102	115-119	0.06652881850492065
1102	120-124	0.11114064071226437
1102	125-129	0.036772759157813084
1102	130-134	0.17261275805616094
1102	135-139	0.22379882581054034
1102	140-144	0.2354711421666451
1102	145-149	0.24348673383813946
1102	150-154	0.7672164119838314
1102	155-159	0.29629685883524104
1102	160-164	0.41737605947709966
1102	165-169	0.4654526686606957
1102	170-174	0.4199900310609763
1102	175-179	0.5605629415271167
1102	180-184	0.620541222397506
1102	185-189	0.9441911510919354
1102	190-194	1.0732225742425356
1102	195-199	0.5306694813727972
1102	200-204	0.366538525518628
1102	205-209	0.512848182362017
1102	210-214	0.30157785418698424
1102	215-219	-0.19247884930053516
1102	220-224	1.0012187889322526
1102	225-229	1.5091039911993427
1102	230-234	0.9044402749100335
1102	235-239	1.088148846752624
1102	240-244	1.2146874451312577
1102	245-249	1.1660502076509935
1102	250-254	0.7680422567907819
1102	255-259	0.8974725648536754
1102	260-264	1.392465945562865
1102	265-269	1.3292483342722043
1102	270-274	0.9063690753831715
1102	275-279	1.6367600968768379
1102	280-284	0.6235179525935415
1102	285-289	1.0633798238358558
1102	290-294	0.6200964885941911
1102	295-299	0.894890896857568
1102	300-301	0.41333666584115036
1103	1	-0.06986607255408472
1103	2	-0.0293682178084822
1103	3	-0.005772305702997471
1103	4	0.01691512400283557
1103	5	0.058224824001612774
1103	6	0.10332941726380795
1103	7	0.06339392189915571
1103	8	0.05226480096685293
1103	9	0.026295741966201547
1103	10-14	0.052929081634921715
1103	15-19	0.014513638469537682
1103	20-24	0.0537668011811121
1103	25-29	0.02670303444062938
1103	30-34	0.09100871122761589
1103	35-39	-0.49336041131044794
1103	40-44	-0.31325034850863176
1103	45-49	-0.20078240261949531
1103	50-54	0.06821192126356124
1103	55-59	-0.3583083051756901
1103	60-64	0.14025250904607844
1103	65-69	0.25870692441135645
1103	70-74	0.15288136523569307
1103	75-79	0.17051900025702338
1103	80-84	0.1328843220792919
1103	85-89	0.14699601574167076
1103	90-94	0.22328015935972445
1103	95-99	0.21241237686216863
1103	100-104	0.21102569190679787
1103	105-109	0.018000894252054422
1103	110-114	0.22106903550265855
1103	115-119	-0.033405761338940465
1103	120-124	0.24791446495331826
1103	125-129	0.34380398285144764
1103	130-134	0.23114263245908262
1103	135-139	0.363842695797608
1103	140-144	0.3510406582980039
1103	145-149	0.6077793543483239
1103	150-154	0.7785701762884898
1103	155-159	-0.10745277437896306
1103	160-164	-0.542819623754653
1103	165-169	0.2866526474953517
1103	170-174	0.7265383000725052
1103	175-179	0.5743406188229585
1103	180-184	0.4548449547136144
1103	185-189	0.4189750481129124
1103	190-194	0.1898575730640104
1103	195-199	0.4096657630065721
1103	200-204	0.6047068573527525
1103	205-209	0.7226342680720421
1103	210-214	1.0194423671194848
1103	215-219	0.4841209380839615
1103	220-224	0.6888620759102686
1103	225-229	1.6683914887340592
1103	230-234	0.9488230405471363
1103	235-239	1.1761836926063793
1103	240-244	1.2387553281638723
1103	245-249	1.6647716284228622
1103	250-254	1.455698531593928
1103	255-259	0.6773919441318412
1103	260-264	1.3682013210312682
1103	265-269	0.6351804031365411
1103	270-274	1.0616034094204139
1103	275-279	1.468766908269565
1103	280-284	0.6920508574889865
1103	285-289	0.5898783542874888
1103	290-294	1.02544795357276
1103	295-299	1.2135904788420095
1103	300-301	-0.6598641596072881
2116	1	-0.002120262272384821
2116	2	-0.05579318402509159
2116	3	0.022949931277402413
2116	4	0.0519193397083626
2116	5	0.04045880143314662
2116	6	-0.1651382604021947
2116	7	-0.03686826033482049
2116	8	0.04199811428040334
2116	9	0.14123666433946624
2116	10-14	0.12544938054575994
2116	15-19	0.11856371874119276
2116	20-24	-0.10031342427249967
2116	25-29	-0.14620164441500805
2116	30-34	0.0753087308624032
2116	35-39	-0.04663699624248352
2116	40-44	-0.05167194216082294
2116	45-49	0.11372493732807243
2116	50-54	0.2235559343842155
2116	55-59	0.13256197814282444
2116	60-64	0.4022901385490911
2116	65-69	0.34213238727272
2116	70-74	-0.6022725368906379
2116	75-79	0.18224998049519314
2116	80-84	-0.019650991673145768
2116	85-89	0.16477751514685224
2116	90-94	0.28000946486555023
2116	95-99	0.2471698871704362
2116	100-104	0.25838920122176745
2116	105-109	0.2841830820299691
2116	110-114	0.5318127321566664
2116	115-119	0.3139482770759514
2116	120-124	-0.06983931474429994
2116	125-129	0.0643015516020995
2116	130-134	0.25773691988935354
2116	135-139	0.5431259103596844
2116	140-144	0.6496057014367977
2116	145-149	0.7597431801299734
2116	150-154	1.1227856508152314
2116	155-159	-0.11138342891054975
2116	160-164	-0.48691059059495956
2116	165-169	0.8305234736581326
2116	170-174	0.9523871583670456
2116	175-179	0.7862180515160304
2116	180-184	0.7247854728411554
2116	185-189	1.1236199471092334
2116	190-194	0.9938928694916385
2116	195-199	0.9141672125162685
2116	200-204	1.1287051941702941
2116	205-209	1.0933252972816732
2116	210-214	1.0938954226397541
2116	215-219	1.337798534785314
2116	220-224	1.667690687268422
2116	225-229	1.1302463991647542
2116	230-234	1.4195450234302207
2116	235-239	1.700557169466201
2116	240-244	2.4065367120702668
2116	245-249	1.3755763084334873
2116	250-254	1.3504287841897558
2116	255-259	1.1454251153818866
2116	260-264	0.28356011799596104
2116	265-269	0.8381117622106977
2116	270-274	1.0100909528768298
2116	275-279	1.577318438050927
2116	280-284	0.31454447618130743
2116	285-289	-0.8659061711857632
2116	290-294	0.5373902965810391
2116	295-299	0.9291569426158173
2116	300-301	1.2257504671583987
1117	1	-0.05282079122545014
1117	2	0.058885699076860476
1117	3	0.048000591162320916
1117	4	0.03710517003694491
1117	5	-0.014756699040063381
1117	6	-0.5341315076862969
1117	7	-0.29410086613994935
1117	8	-0.07947645126390768
1117	9	0.03723206575736526
1117	10-14	0.09599430091249417
1117	15-19	0.04196759850452736
1117	20-24	-0.020629983232481663
1117	25-29	0.14410312777518186
1117	30-34	0.11352016112595464
1117	35-39	-0.0417778278194092
1117	40-44	0.032523683379885426
1117	45-49	0.10394412479577397
1117	50-54	0.0102033241663122
1117	55-59	0.2559010597037883
1117	60-64	0.2396341090066798
1117	65-69	0.24584481331509522
1117	70-74	-0.04464010991925704
1117	75-79	0.16209309452492704
1117	80-84	0.1528267266869321
1117	85-89	0.19777071598063145
1117	90-94	0.37292311951900103
1117	95-99	0.4076239390902927
1117	100-104	0.3209998255721942
1117	105-109	0.24582068821250402
1117	110-114	0.36736813287780734
1117	115-119	0.3815200283112361
1117	120-124	0.4322670913360511
1117	125-129	0.4109138713893614
1117	130-134	0.19051912012508865
1117	135-139	0.4288265264071143
1117	140-144	0.4282041790274249
1117	145-149	0.8371157175369888
1117	150-154	0.984480739274062
1117	155-159	-0.0495717501377797
1117	160-164	-0.5072031115121192
1117	165-169	0.4191178287824755
1117	170-174	0.7318895592681898
1117	175-179	0.88304770075802
1117	180-184	0.7266060933013634
1117	185-189	0.9095993812281478
1117	190-194	1.0410018593712707
1117	195-199	0.7798498290630391
1117	200-204	1.0158912915882397
1117	205-209	1.423179501326345
1117	210-214	0.8937012602842529
1117	215-219	0.6501309797561987
1117	220-224	1.4327848943323573
1117	225-229	0.6585264017195271
1117	230-234	1.347299905068983
1117	235-239	0.8891712659783657
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2108	185-189	-1.0125116367943434
2108	190-194	-0.6342497406134342
2108	195-199	-1.591472929249175
2108	200-204	-1.0978992265831593
2108	205-209	-0.5800388926748852
2108	210-214	-0.09366688510084842
2108	215-219	-1.1100834871415337
2108	220-224	-0.40870079995613295
2108	225-229	0.013864956512989579
2108	230-234	-0.3339024908233732
2108	235-239	-0.5947185711783618
2108	240-244	-0.8856332010099379
2108	245-249	-0.062131474398285036
2108	250-254	-0.46002533322901584
2108	255-259	-1.2721490061460337
2108	260-264	-0.12473532941556087
2108	265-269	-0.8030801176653455
2108	270-274	-0.827594457951907
2108	275-279	-0.3660187571162652
2108	280-284	-0.7391898791112119
2108	285-289	0.015688404744928164
2108	290-294	-0.5938895303783625
2108	295-299	-0.28447651037729393
2108	300-301	-0.250277246731077
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	48.0
14	198.0
15	360.0
16	447.0
17	568.0
18	1116.0
19	1016.0
20	953.0
21	1141.0
22	1641.0
23	2546.0
24	3585.0
25	5222.0
26	7529.0
27	10345.0
28	14429.0
29	20127.0
30	28410.0
31	40945.0
32	55513.0
33	67644.0
34	58368.0
35	24002.0
36	1935.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.85167224944986	22.97454135951783	15.159985521152333	34.01380086987997
2	43.59741908794751	24.14060569846076	17.655575792745637	14.606399420846095
3	42.92691054715106	22.827741931777048	19.520014708670647	14.725332812401248
4	43.565818428355556	22.209518118669095	19.06352881692876	15.161134636046587
5	27.209891581009728	22.46979264221733	35.731153079340636	14.589162697432304
6	25.731267990830066	22.32385505064724	36.299677673272164	15.645199285250536
7	17.265738564870407	47.371686958120506	20.131056553689522	15.231517923319563
8	25.132291852200844	21.15577976063937	37.45539997816682	16.256528408992974
9	15.91610312157061	19.530644021442484	38.96648606410912	25.586766792877786
10-14	26.408958499715595	33.848041046384026	23.584606456876593	16.158393997023794
15-19	20.841324469827114	36.12891919998621	21.511545731900004	21.518210598286668
20-24	25.516555872838946	30.63672456290542	26.160347492344023	17.68637207191161
25-29	19.568047711250408	25.529425959654578	37.53175866288991	17.370767666205104
30-34	19.423029411595717	28.251851511373367	31.03288192269904	21.292237154331875
35-39	20.78427091532747	30.14806345412446	27.743770360879534	21.32389526966854
40-44	20.674530442926336	29.155458008468976	28.560561227714352	21.609450320890332
45-49	21.109355518911556	30.420748418530625	26.596436594712923	21.873459467844892
50-54	22.144708038633244	29.83665331778198	27.387372376427056	20.63126626715772
55-59	21.401632892264733	27.22804759633892	29.936856136560813	21.433463374835533
60-64	22.593494860583636	25.637787494182607	28.340620636954384	23.428097008279373
65-69	20.445282021522925	27.99703528357283	28.284428918625427	23.27325377627882
70-74	21.074996983573403	26.814193867173806	29.22158957063322	22.889219578619567
75-79	20.467804673450274	26.570236775123963	30.006090308939537	22.955868242486225
80-84	23.023436198268286	26.132251633179543	27.81231506432171	23.031997104230467
85-89	21.40220744971186	27.939004981413067	26.55558556022224	24.103202008652836
90-94	20.330198164863514	30.013617011496898	28.967232988790386	20.688951834849206
95-99	22.10322499095072	30.63804604503381	26.796037851844616	20.46269111217085
100-104	22.84802380966061	28.08138031681098	26.791269025033472	22.279326848494946
105-109	23.44464426275661	27.62024050974737	24.1416973576103	24.79341786988572
110-114	21.68000597539745	27.846213953702158	29.68146535131315	20.79231471958724
115-119	23.727039248019214	27.033674811976077	27.598809516969553	21.640476423035157
120-124	18.367452469735188	29.16447856038886	27.864255057541925	24.603813912334026
125-129	20.782604698730804	25.198653237343937	29.981384338713106	24.037357725212154
130-134	24.800772205208936	29.039627227128307	25.844915453871653	20.314685113791104
135-139	24.388584692640496	27.13560130309629	25.043350359385684	23.432463644877533
140-144	20.908777514119752	29.33362827282286	26.98489488471505	22.772699328342345
145-149	21.145265359356955	26.826719219521163	29.04560262457842	22.98241279654346
150-154	23.029986153165524	25.832677380247866	25.50948881623929	25.62784765034732
155-159	21.273104391342567	27.2311502065534	30.105776026015963	21.389969376088068
160-164	24.97526530190121	29.093233436945194	25.78941320447925	20.142088056674346
165-169	20.361798824455462	25.962010261596006	28.13648037599039	25.539710537958136
170-174	22.50139330180928	29.09122248588025	26.307146920084808	22.100237292225664
175-179	20.40098364234948	28.93620688664556	28.97843685900935	21.684372611995613
180-184	19.907840985480934	30.09371031962631	26.314099065195034	23.684349629697728
185-189	21.765902313742842	28.49132705533563	27.38352284153131	22.35924778939022
190-194	20.540486190511757	28.21686096284337	26.268077013680212	24.97457583296466
195-199	21.097059989543055	30.38374691893569	27.310956235959257	21.208236855562003
200-204	21.394221101196802	28.314708096088985	27.309290019362585	22.981780783351624
205-209	21.181060288312928	27.91033456480146	27.60260159612059	23.306003550765023
210-214	22.75167052577752	27.946186949502145	27.251317172947537	22.050825351772797
215-219	22.708636172987756	27.945095290352604	26.915947991059884	22.430320545599752
220-224	19.553166673369837	27.75853648727068	27.766350468551597	24.921946370807884
225-229	21.302694099869576	29.149080420805873	26.262101616230098	23.286123863094453
230-234	20.5897832194752	27.83794032646354	27.80605238814803	23.76622406591323
235-239	21.721833757548247	27.679247559567244	25.720925956781787	24.87799272610272
240-244	22.166541221624044	31.78963153630916	23.069056059570116	22.97477118249668
245-249	20.392078001919025	30.181904887760204	24.08366705545054	25.342350054870238
250-254	20.746522491051266	27.526645101610487	25.686797244422483	26.040035162915764
255-259	20.98140157543652	28.818135331261097	26.35282423713135	23.847638856171034
260-264	20.79363620171563	29.633604715967525	25.75252661637374	23.820232465943107
265-269	19.72047780197303	29.17665917826794	26.132941102116096	24.969921917642935
270-274	18.9632110866605	31.30866949731969	24.23132831936201	25.4967910966578
275-279	20.342838428700293	29.755238527524174	23.862922084264596	26.03900095951094
280-284	17.61466730251024	31.072813665274325	25.840548817273497	25.47197021494194
285-289	19.282090469815625	29.03468603308302	27.363872976839588	24.319350520261768
290-294	19.07335374927462	31.866277499755814	24.62961154171	24.430757209259568
295-299	18.159232850896597	32.696168276385116	22.708004159795916	26.436594712922375
300-301	14.163559268473458	35.79923813682511	27.141231966078127	22.895970628623303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.25
28	0.0
29	0.25
30	0.25
31	0.75
32	2.5
33	6.75
34	22.25
35	47.0
36	151.0
37	520.25
38	1970.0
39	3499.75
40	5091.0
41	7020.5
42	10305.25
43	16220.5
44	17295.0
45	14650.5
46	11517.0
47	8600.5
48	7171.0
49	4982.0
50	3263.5
51	2683.75
52	862.0
53	155.5
54	58.0
55	33.75
56	27.25
57	18.75
58	14.5
59	14.0
60	12.75
61	6.5
62	4.5
63	3.0
64	2.25
65	1.5
66	1.0
67	0.75
68	0.75
69	0.5
70	0.75
71	0.25
72	0.0
73	0.25
74	0.5
75	0.25
76	0.25
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.25
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	348094.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.081296621123832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	36.14046440092217	3.643427416508629
2	6.0516888976300995	1.2201774167154187
3	5.285705805332988	1.5986030422667423
4	4.512900052577423	1.8198353620607337
5	5.158899022544978	2.6004195642350862
6	4.91052730413541	2.9702689391469983
7	4.817940815210886	3.399976332281207
8	4.128767926529734	3.3298667317702915
9	3.798380924969897	3.446334430617621
>10	24.118101220509594	44.26504864221664
>50	0.9025352600354614	5.9161065246301625
>100	0.11431985461964102	1.8405280590608246
>500	0.014230598805714586	1.0624861459687411
>1k	0.02561507784903856	6.387254683936885
>5k	0.014230598805021425	8.82153296157583
>10k+	0.00569223952200857	7.6781337470082685
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	15121	4.343941578998776	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	11639	3.343637063551799	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	7193	2.066395858589921	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	6945	1.9951507351462536	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	5892	1.6926462392342296	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5457	1.5676799944842483	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	5258	1.5105115284951767	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	4270	1.2266801496147592	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	4162	1.1956540474699362	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	2862	0.8221917068378082	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	2489	0.7150367429487438	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2465	0.7081420535832275	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1623	0.46625336834303377	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1605	0.46108235131889663	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1527	0.43867461088096893	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT	1258	0.36139663424247476	TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	978	0.2809585916447856	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	753	0.2163208788430711	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	750	0.2154590426723816	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	713	0.20482972990054413	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	509	0.1462248702936563	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	493	0.1416284107166455	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	405	0.11634788304308608	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCCTTAATTGTCGTACTCT	382	0.10974047240113302	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.872787235631755E-4	0.0	0.0
2	2.872787235631755E-4	0.0	0.0
3	2.872787235631755E-4	0.0	0.0
4	2.872787235631755E-4	0.0	0.0
5	2.872787235631755E-4	0.0	0.0
6	2.872787235631755E-4	0.0	0.0
7	2.872787235631755E-4	0.0	0.0
8	2.872787235631755E-4	0.0	0.0
9	2.872787235631755E-4	0.0	0.0
10-14	0.001321482128390607	0.0	0.0
15-19	0.002010951064942228	0.0	0.0
20-24	0.002470597022643309	0.0	0.0
25-29	0.002642964256781214	0.0	0.0
30-34	0.005630662981838239	0.0	0.0
35-39	0.00643504340781513	0.0	0.0
40-44	0.007813981280918373	0.0	0.0
45-49	0.009652565111722694	0.0	0.0
50-54	0.01534068383827357	0.0	0.0
55-59	0.01976477618114647	0.0	0.0
60-64	0.02642964256781214	0.0	0.0
65-69	0.03573747321125903	0.0	0.0
70-74	0.05084833407068205	0.0	0.0
75-79	0.07124512344366751	0.0	0.0
80-84	0.10129447792837568	0.0	0.0
85-89	0.1414560434825076	0.0	0.0
90-94	0.18925922308342	0.0	0.0
95-99	0.2498175780105374	0.0	0.0
100-104	0.32950869592696225	0.0	0.0
105-109	0.42930932449280945	0.0	0.0
110-114	0.5584698386068132	0.0	0.0
115-119	0.7147494642251806	0.0	0.0
120-124	0.8970565421983718	0.0	0.0
125-129	1.1044143248662717	0.0	0.0
130-134	1.3590007296879576	0.0	0.0
135-139	1.6459347187828577	0.0	0.0
140-144	1.9734899193895898	0.0	0.0
145-149	2.3632696915201064	0.0	0.0
150-154	2.8161933270898087	0.0	0.0
155-159	3.332433193332835	0.0	0.0
160-164	3.9268703281297577	0.0	0.0
165-169	4.596287209776669	0.0	0.0
170-174	5.357116181261384	0.0	0.0
175-179	6.188902997466203	0.0	0.0
180-184	7.10572431584572	0.0	0.0
185-189	8.174228800266595	0.0	0.0
190-194	9.40803346222572	0.0	0.0
195-199	10.67378351824507	0.0	0.0
200-204	12.023361505800157	0.0	0.0
205-209	13.48865536320649	0.0	0.0
210-214	15.032548679379707	0.0	0.0
215-219	16.628324533028437	0.0	0.0
220-224	18.302584933954623	0.0	0.0
225-229	20.074002999189876	0.0	0.0
230-234	21.910058777226844	0.0	0.0
235-239	23.738185662493464	0.0	0.0
240-244	25.537929409872046	0.0	0.0
245-249	27.231954586979384	0.0	0.0
250-254	29.038650479468185	0.0	0.0
255-259	30.77559509787586	0.0	0.0
260-264	32.487891201801816	0.0	0.0
265-269	34.00765310519572	0.0	0.0
270-274	35.38969358851345	0.0	2.872787235631755E-4
275-279	36.59890719173556	0.0	2.872787235631755E-4
280-284	37.673731808074834	0.0	2.872787235631755E-4
285-289	38.62973794434836	0.0	2.872787235631755E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	6060	0.0	294.51318	1
GGGTTAT	6120	0.0	291.6258	2
GGGGGGA	3205	0.0	289.4774	1
GTTATTA	6180	0.0	288.7945	4
GGTTATT	6210	0.0	287.39932	3
TTAGTAG	6305	0.0	282.83505	8
TATTAGT	6325	0.0	282.17392	6
TAGTAGA	6325	0.0	281.9407	9
TTATTAG	6360	0.0	280.62106	5
GGGGAGC	3350	0.0	276.06717	3
GGAGCGA	2615	0.0	271.30975	5
GGGAGCG	2630	0.0	270.3232	4
AAACGTT	255	0.0	266.07843	295
GCGAAAG	2665	0.0	265.11258	8
ATTAGTA	6755	0.0	264.2117	7
CGAAAGC	2700	0.0	261.6759	9
GAGCGAA	2765	0.0	256.05786	6
AGCGAAA	2800	0.0	252.33037	7
AACCGTT	40	3.45608E-11	221.24998	295
GGGGGAG	4525	0.0	205.35912	2
>>END_MODULE