##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_6628_flu.3510000003bad6.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	439231
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.77575580958539	34.0	34.0	34.0	34.0	34.0
2	33.86887992878463	34.0	34.0	34.0	34.0	34.0
3	33.876538768893816	34.0	34.0	34.0	34.0	34.0
4	33.886342266370086	34.0	34.0	34.0	34.0	34.0
5	33.88351459710266	34.0	34.0	34.0	34.0	34.0
6	37.648540289733646	38.0	38.0	38.0	38.0	38.0
7	37.65580981305964	38.0	38.0	38.0	38.0	38.0
8	37.64985622599498	38.0	38.0	38.0	38.0	38.0
9	37.559231930351	38.0	38.0	38.0	37.0	38.0
10-14	37.65135703081067	38.0	38.0	38.0	37.8	38.0
15-19	37.619788676118034	38.0	38.0	38.0	37.2	38.0
20-24	37.61226142963498	38.0	38.0	38.0	37.0	38.0
25-29	37.50610954144858	38.0	38.0	38.0	37.0	38.0
30-34	37.43477668925918	38.0	38.0	38.0	37.0	38.0
35-39	37.339155478552286	38.0	38.0	38.0	37.0	38.0
40-44	37.35465165254729	38.0	38.0	38.0	37.0	38.0
45-49	37.312456543367844	38.0	38.0	38.0	37.0	38.0
50-54	37.270945356771264	38.0	38.0	38.0	37.0	38.0
55-59	37.22220517222145	38.0	38.0	38.0	37.0	38.0
60-64	37.06239723516783	38.0	38.0	38.0	36.4	38.0
65-69	36.9836414096455	38.0	38.0	38.0	36.2	38.0
70-74	37.06768283659395	38.0	38.0	38.0	36.8	38.0
75-79	36.99605036985095	38.0	38.0	38.0	36.0	38.0
80-84	36.88654990198779	38.0	38.0	38.0	35.8	38.0
85-89	36.92943029977393	38.0	38.0	38.0	36.0	38.0
90-94	36.78679009450608	38.0	38.0	38.0	35.4	38.0
95-99	36.78286459744417	38.0	38.0	38.0	35.6	38.0
100-104	36.74880324931529	38.0	38.0	38.0	35.6	38.0
105-109	36.599933520175036	38.0	38.0	38.0	35.0	38.0
110-114	36.503534131243015	38.0	37.6	38.0	34.0	38.0
115-119	36.58852130200282	38.0	38.0	38.0	34.6	38.0
120-124	36.492308147648956	38.0	37.2	38.0	34.2	38.0
125-129	36.41321127151772	38.0	37.0	38.0	34.0	38.0
130-134	36.26211902165375	38.0	37.0	38.0	34.0	38.0
135-139	36.00338591766064	38.0	37.0	38.0	32.4	38.0
140-144	35.9861790265259	38.0	37.0	38.0	32.4	38.0
145-149	35.54678198943153	38.0	36.2	38.0	29.4	38.0
150-154	35.13517078712569	38.0	36.0	38.0	27.6	38.0
155-159	35.28689687203317	38.0	36.0	38.0	28.6	38.0
160-164	35.20012021009446	38.0	35.8	38.0	28.0	38.0
165-169	34.964218827906045	38.0	35.2	38.0	27.0	38.0
170-174	34.86608868681855	38.0	35.0	38.0	26.6	38.0
175-179	34.529481297995815	38.0	35.0	38.0	24.2	38.0
180-184	34.23791626729444	37.6	34.6	38.0	23.4	38.0
185-189	33.43776418331129	37.0	33.4	37.8	22.2	38.0
190-194	32.468006584234715	36.8	30.4	37.0	20.2	38.0
195-199	32.23222814418837	36.8	30.0	37.2	20.2	37.8
200-204	32.09265784974193	37.0	29.8	37.0	19.8	37.4
205-209	30.965372207335093	35.4	27.4	37.0	17.6	37.2
210-214	30.28740548822829	34.2	26.0	37.0	16.0	37.0
215-219	29.446073706090875	33.2	24.2	37.0	14.4	37.0
220-224	28.016397294362196	31.4	22.0	36.2	9.6	37.0
225-229	27.001367389824487	30.0	20.2	36.2	9.2	37.0
230-234	26.6373306984252	29.2	20.0	35.8	9.0	37.0
235-239	25.52910199872049	27.6	18.4	34.4	9.0	37.0
240-244	24.56319294403173	26.4	16.8	33.4	8.4	37.0
245-249	23.73107271572362	25.0	16.0	33.0	8.0	36.2
250-254	22.860594083750918	23.8	14.4	31.8	8.0	36.0
255-259	22.390405504165233	23.0	13.8	31.2	8.0	36.0
260-264	21.111290869724588	21.2	11.8	29.4	7.8	35.6
265-269	20.286032178967332	19.6	11.4	28.6	7.0	34.0
270-274	19.351234771680506	19.0	9.2	27.0	7.0	33.4
275-279	18.050430411332535	17.0	8.0	25.2	7.0	31.8
280-284	17.85714441831292	17.2	8.0	25.0	7.0	31.0
285-289	16.742791378568448	15.4	8.0	22.6	7.0	30.0
290-294	16.02118156505347	13.8	8.0	21.6	7.0	29.0
295-299	15.209328121193632	13.0	8.0	21.0	7.0	27.0
300-301	13.295073662833452	11.0	8.0	17.0	7.0	23.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.1128053374703768
1101	2	-0.004496717589091759
1101	3	-0.03579301972544613
1101	4	-0.01262600374816003
1101	5	0.023229537252802857
1101	6	0.08677512520095831
1101	7	0.1131894078464768
1101	8	0.07175225370437488
1101	9	0.07706570296196702
1101	10-14	0.03248759080571517
1101	15-19	-0.015169465511092994
1101	20-24	0.008389527529715224
1101	25-29	0.08343801639641413
1101	30-34	0.09151680398035467
1101	35-39	0.1289876051244221
1101	40-44	0.10848391577501815
1101	45-49	0.19955009113441236
1101	50-54	0.10354180939711455
1101	55-59	0.05821699315595197
1101	60-64	0.3068299844010838
1101	65-69	0.22986250585309875
1101	70-74	0.2252149781872248
1101	75-79	0.12512887916648907
1101	80-84	0.22001498790864105
1101	85-89	0.13874072351812572
1101	90-94	0.29708489435181207
1101	95-99	0.0954178363874334
1101	100-104	0.191065897844112
1101	105-109	0.23130563255605807
1101	110-114	0.1806693176184524
1101	115-119	0.1569774938436055
1101	120-124	0.1354668054518342
1101	125-129	0.1895924244395033
1101	130-134	0.19470760409272003
1101	135-139	0.2762310768312801
1101	140-144	0.2392359283487835
1101	145-149	0.4508874408468273
1101	150-154	0.6275669438166247
1101	155-159	0.30000530107160017
1101	160-164	0.3406625611349199
1101	165-169	0.2815025496260404
1101	170-174	0.2662233226296209
1101	175-179	0.4006766816039189
1101	180-184	0.05092017486900602
1101	185-189	0.17475107214561802
1101	190-194	0.24889705499947468
1101	195-199	0.010948811248702839
1101	200-204	-0.14695714140292182
1101	205-209	-0.2928425308014475
1101	210-214	-0.4023593613833505
1101	215-219	-0.25275842574044205
1101	220-224	0.25717156110776784
1101	225-229	0.6190820624592668
1101	230-234	0.24982320960305415
1101	235-239	0.22306281749778023
1101	240-244	0.29855931011503856
1101	245-249	0.7133578851303355
1101	250-254	0.30848961783649287
1101	255-259	-0.04089263064178894
1101	260-264	0.3522359241341455
1101	265-269	0.026807756885450118
1101	270-274	0.28614724003540815
1101	275-279	0.327045468119767
1101	280-284	0.05844529244149754
1101	285-289	0.2864664269893815
1101	290-294	0.21455901107681896
1101	295-299	0.2531883955949148
1101	300-301	-0.058506359173049916
1102	1	-0.06337861243389398
1102	2	-0.01400414782823134
1102	3	0.033656636309579824
1102	4	0.012018863579633887
1102	5	0.01279891096852026
1102	6	-0.030737308165129207
1102	7	0.08694085964670961
1102	8	-0.04166684751960048
1102	9	-0.038073223152572666
1102	10-14	0.033399172449016135
1102	15-19	0.024058027862750464
1102	20-24	0.03520777703088385
1102	25-29	0.07040844434244775
1102	30-34	0.12056055156068624
1102	35-39	0.050721129053073355
1102	40-44	0.032551636672010886
1102	45-49	0.11209692099831159
1102	50-54	0.09585962708077744
1102	55-59	-0.018538419453129507
1102	60-64	0.1987535080319205
1102	65-69	0.22569442461185218
1102	70-74	0.12410863195893285
1102	75-79	-0.03054490504388241
1102	80-84	0.22557411712630682
1102	85-89	0.2166876546357841
1102	90-94	0.29092360306857046
1102	95-99	0.08428677232954129
1102	100-104	0.17188784012947167
1102	105-109	0.23315262025669625
1102	110-114	0.3356060340185749
1102	115-119	0.17636997128486342
1102	120-124	0.2077473974885251
1102	125-129	0.16108829983781447
1102	130-134	0.19443809040856053
1102	135-139	0.17166721251132344
1102	140-144	0.2653317024933344
1102	145-149	0.4890419421720651
1102	150-154	0.89985825683965
1102	155-159	0.5801556926854019
1102	160-164	0.4730759742800501
1102	165-169	0.46262855099595157
1102	170-174	0.3894348536460228
1102	175-179	0.5408779979027969
1102	180-184	0.6681416525782922
1102	185-189	0.7253469803025041
1102	190-194	0.7254642547374104
1102	195-199	0.6742085867035996
1102	200-204	0.32177331513241114
1102	205-209	0.25900803056131494
1102	210-214	-0.05079499079458927
1102	215-219	0.07860552196496684
1102	220-224	0.895143210055025
1102	225-229	1.257538836037984
1102	230-234	0.809402113084392
1102	235-239	1.258394721366269
1102	240-244	1.3210039928036252
1102	245-249	1.2752864714491636
1102	250-254	0.8114232966238291
1102	255-259	0.7542317030191334
1102	260-264	1.343593320125688
1102	265-269	1.0517274983905516
1102	270-274	1.009414460619702
1102	275-279	1.310165870752364
1102	280-284	0.6120266974863178
1102	285-289	0.8726789407628708
1102	290-294	0.8276758400473057
1102	295-299	0.6594277118342315
1102	300-301	0.38022171492087686
1103	1	0.04973451105077942
1103	2	0.03452397096430815
1103	3	0.0763913690944591
1103	4	0.03568456418857835
1103	5	0.07304109641015799
1103	6	0.06628118508085379
1103	7	0.13326870549586545
1103	8	0.12525638197485733
1103	9	0.24665156880951145
1103	10-14	0.13309292524282768
1103	15-19	0.11526877745051678
1103	20-24	0.13062975874190386
1103	25-29	0.1283405282148138
1103	30-34	0.189725054711964
1103	35-39	-0.27273675566534905
1103	40-44	-0.12039567815130425
1103	45-49	-0.06901507163635756
1103	50-54	0.13423363055622417
1103	55-59	-0.24657151013722256
1103	60-64	0.2870687487519916
1103	65-69	0.31482020651173315
1103	70-74	0.19450093625823683
1103	75-79	0.3433722764753142
1103	80-84	0.2910170284433917
1103	85-89	0.279994236360416
1103	90-94	0.369569336182046
1103	95-99	0.3713292147287177
1103	100-104	0.3089740955654037
1103	105-109	0.16619238650823576
1103	110-114	0.4117625649733796
1103	115-119	0.2599110711697037
1103	120-124	0.4735046969476713
1103	125-129	0.43136500284759194
1103	130-134	0.511150247638156
1103	135-139	0.5493860630703722
1103	140-144	0.6211813349670905
1103	145-149	0.7889731142596048
1103	150-154	1.0890046131047555
1103	155-159	0.058308825169525846
1103	160-164	-0.4441862141811441
1103	165-169	0.5723294382099411
1103	170-174	1.0734401348931613
1103	175-179	0.8529413847097445
1103	180-184	0.8617029123807214
1103	185-189	0.8846070728427478
1103	190-194	0.7236597792949127
1103	195-199	0.8689021766787022
1103	200-204	0.7730197664244471
1103	205-209	1.302775330050057
1103	210-214	1.0985917747079732
1103	215-219	0.8378015231372622
1103	220-224	1.6083671982847179
1103	225-229	2.2766537534888904
1103	230-234	1.4840482784075597
1103	235-239	1.8587341243840427
1103	240-244	1.5983829962938714
1103	245-249	1.5039866748245458
1103	250-254	1.5964414583116984
1103	255-259	0.8507417578150331
1103	260-264	1.934233760150672
1103	265-269	0.6218624010989693
1103	270-274	0.9916475341338291
1103	275-279	1.870045562521728
1103	280-284	0.9277207936796152
1103	285-289	0.998700776233072
1103	290-294	1.2303539119184457
1103	295-299	1.0287989947569187
1103	300-301	-0.7104187251041072
2116	1	-0.017532445781100137
2116	2	0.023886351749609958
2116	3	-0.006528995922167269
2116	4	0.014034537076206277
2116	5	0.006317836894005779
2116	6	-0.28051433543321025
2116	7	-0.09801650796694616
2116	8	0.07564653916863051
2116	9	0.14851927782850538
2116	10-14	0.09889726380242081
2116	15-19	0.09709913808858062
2116	20-24	-0.08447328277337363
2116	25-29	-0.170133349666429
2116	30-34	0.02664384228145167
2116	35-39	-0.15712394354684278
2116	40-44	-0.1499732470848727
2116	45-49	0.03197300761875965
2116	50-54	0.19252871176285424
2116	55-59	0.14306924367372886
2116	60-64	0.3103260901156162
2116	65-69	0.21023676564715998
2116	70-74	-0.7868645994213566
2116	75-79	0.15003684567855657
2116	80-84	-0.0728008576034469
2116	85-89	0.1488956198163578
2116	90-94	0.24525997675898026
2116	95-99	0.19564886815430782
2116	100-104	0.24257197993040336
2116	105-109	0.2067229190996258
2116	110-114	0.31896691687167333
2116	115-119	0.24499622141865274
2116	120-124	-0.2341405572271924
2116	125-129	-0.05763665289195785
2116	130-134	0.12056157568046899
2116	135-139	0.39795302193206084
2116	140-144	0.438541082807518
2116	145-149	0.8101333788299669
2116	150-154	1.031104763600176
2116	155-159	-0.40576670091200384
2116	160-164	-0.6583298836595688
2116	165-169	0.5469592165844261
2116	170-174	0.7107123982108376
2116	175-179	0.5039227946111282
2116	180-184	0.6167152216879401
2116	185-189	0.8984957120336006
2116	190-194	0.690899913725886
2116	195-199	0.8809905253177277
2116	200-204	0.7502424735441267
2116	205-209	0.91116581201236
2116	210-214	0.6455000942918083
2116	215-219	0.9884139437937023
2116	220-224	1.4340260869384025
2116	225-229	0.8277270844831257
2116	230-234	1.2523056342697814
2116	235-239	1.1245706498247259
2116	240-244	1.431760791985468
2116	245-249	1.2561833569660443
2116	250-254	1.2087868670415922
2116	255-259	0.8166777436180084
2116	260-264	0.27900614670641843
2116	265-269	0.6354326511417376
2116	270-274	0.8471842576253259
2116	275-279	1.5911144859430486
2116	280-284	0.49253531315267907
2116	285-289	-1.2105838340881832
2116	290-294	0.4551222968619477
2116	295-299	0.7191546742915698
2116	300-301	1.4261228815142104
1117	1	-0.1162241408891802
1117	2	-0.0024528677191568704
1117	3	-0.007279674588055229
1117	4	-0.013163743927847804
1117	5	0.020049000824187146
1117	6	-0.49821624035306655
1117	7	-0.266435485589156
1117	8	0.028995351612437048
1117	9	0.09165682369118855
1117	10-14	0.13849629082976378
1117	15-19	0.08932175795712283
1117	20-24	0.03835302240369032
1117	25-29	0.1453489613764134
1117	30-34	0.15396636351431425
1117	35-39	-0.05957514215954518
1117	40-44	-0.060286495399481055
1117	45-49	0.09221802564378123
1117	50-54	0.11406096912342889
1117	55-59	0.16612002504875534
1117	60-64	0.2573382144489571
1117	65-69	0.2856152328078778
1117	70-74	-0.11445756864644352
1117	75-79	0.1799355331547332
1117	80-84	0.012623464967241205
1117	85-89	0.1771484879616878
1117	90-94	0.2665578652570062
1117	95-99	0.2759857424796337
1117	100-104	0.25555668412518173
1117	105-109	0.2544800483647407
1117	110-114	0.29509999066804227
1117	115-119	0.2986969508571775
1117	120-124	0.5863024886660355
1117	125-129	0.3896531934516787
1117	130-134	0.2020829511100004
1117	135-139	0.2842337873915355
1117	140-144	0.36504876853502566
1117	145-149	0.8031929472188324
1117	150-154	0.9764326617872001
1117	155-159	-0.10830431485950243
1117	160-164	-0.6340422282501663
1117	165-169	0.31378969413825786
1117	170-174	0.6849887061520405
1117	175-179	0.7172211544655269
1117	180-184	0.6073291197178534
1117	185-189	0.9181808052428693
1117	190-194	0.952512592630363
1117	195-199	0.5714205361705567
1117	200-204	0.7212243786967534
1117	205-209	1.2279133657600845
1117	210-214	0.874568961785382
1117	215-219	0.4327326447122637
1117	220-224	0.9434548146544479
1117	225-229	0.5618765626450681
1117	230-234	1.2386246610643532
1117	235-239	0.6921598074643356
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2108	180-184	-0.46570986176102735
2108	185-189	-0.8949136221344531
2108	190-194	-0.5233110787471169
2108	195-199	-1.3994672683981442
2108	200-204	-0.9566218356830021
2108	205-209	-0.4161429910249872
2108	210-214	0.09763500322640084
2108	215-219	-0.9322587544715404
2108	220-224	-0.6951718220048484
2108	225-229	0.03426192533143535
2108	230-234	-0.33309216562001254
2108	235-239	-0.6094928319809476
2108	240-244	-0.038525314661889354
2108	245-249	-0.1642884002082603
2108	250-254	-0.6881592034277126
2108	255-259	-0.5967074141488773
2108	260-264	-0.056271636681106685
2108	265-269	-0.6123262714793505
2108	270-274	-0.8670395731514091
2108	275-279	-0.1821871795744201
2108	280-284	-0.7451068818799058
2108	285-289	-0.11837625169945554
2108	290-294	-0.6131577166399111
2108	295-299	-0.12717414784455094
2108	300-301	-0.13867917780740946
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	20.0
13	102.0
14	362.0
15	586.0
16	619.0
17	726.0
18	1259.0
19	1199.0
20	1243.0
21	1677.0
22	2318.0
23	3430.0
24	5070.0
25	6977.0
26	9809.0
27	13253.0
28	18374.0
29	25456.0
30	36164.0
31	51343.0
32	70063.0
33	83937.0
34	72359.0
35	30267.0
36	2613.0
37	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.58991282491445	20.436399070193133	15.554457677167596	31.41923042772482
2	45.96487952808431	23.01499666462522	16.20058693489303	14.81953687239744
3	45.75291816834422	21.868219683947625	18.326347639397035	14.052514508311118
4	46.4452645646596	21.492335468124974	18.07272255373596	13.989677413479468
5	30.359423629024363	21.63280824896239	33.980752724648305	14.02701539736494
6	28.55012510501308	22.32378862147708	34.37621661494749	14.74986965856235
7	16.96537121781299	48.703868132868884	19.848143341757165	14.482617307560957
8	28.136675234671504	20.301618055191913	35.698299983380046	15.863406726756535
9	15.13212865212155	18.822441949680236	37.45705562676587	28.588373771432344
10-14	26.81081253372371	34.86265769037249	22.618257818778726	15.708271957125067
15-19	21.94348759536554	35.65882189554016	20.988227151544404	21.4094633575499
20-24	25.279499853152444	31.025132561226325	26.042150941076564	17.65321664454467
25-29	20.048357242544355	25.59778339871275	36.96378443233743	17.390074926405468
30-34	19.702252345576703	28.33921103018685	31.072123779969996	20.886412844266456
35-39	20.72645145720589	29.71802992047465	27.314556577290766	22.2409620450287
40-44	20.425880686927837	29.186874332640457	28.622387764069472	21.76485721636223
45-49	22.32515464527777	29.44965177776614	25.789208867315832	22.43598470964026
50-54	22.236772905373254	29.936229455571212	27.14999624343455	20.677001395620984
55-59	21.825098865972574	27.163610947314737	29.261003890891125	21.75028629582156
60-64	22.34109158961913	25.874448752478763	28.977189679234844	22.80726997866726
65-69	20.06798245114757	27.65747408539015	28.281382689291057	23.993160774171223
70-74	20.92261247498469	25.835289403525707	29.30958880406893	23.932509317420674
75-79	20.282038380715388	25.776368243589364	30.271451696260055	23.670141679435194
80-84	23.588772195040878	25.911149258590584	26.974416650919448	23.52566189544909
85-89	21.43450712722918	27.777820782230762	25.908781484002724	24.878890606537336
90-94	20.172027930633313	29.599959929968513	29.271431205903042	20.956580933495133
95-99	22.46330518565402	29.561301456409044	26.72575478506754	21.2496385728694
100-104	22.28376412411692	28.176517595524906	26.721929918425612	22.81778836193256
105-109	23.625381632899316	27.880363635535744	23.847178364004364	24.647076367560576
110-114	21.23634260787604	28.6262126307114	28.882478695720476	21.254966065692084
115-119	24.27501701837985	27.501656303858336	26.50090726747429	21.722419410287525
120-124	18.682515578363095	28.61756114664038	28.09842656825224	24.601496706744285
125-129	20.73897334204553	24.335122065610122	31.565212837891675	23.360691754452667
130-134	25.34925813524091	28.381739904514937	26.030084397503817	20.238917562740337
135-139	24.286946959572525	27.689256905819487	24.612197226516344	23.41159890809164
140-144	20.89169480296245	28.643424530600072	27.33295236447336	23.131928301964113
145-149	21.39002028545344	26.86381425719041	29.489312002112783	22.25685345524337
150-154	23.57033087373159	25.51450148099747	25.470788719375452	25.444378925895485
155-159	21.68016374071958	26.106991537482553	30.58222211091658	21.63062261088129
160-164	24.927657656221896	29.83746593478147	25.758792070687182	19.476084338309455
165-169	21.10529539126337	26.05266932434186	28.315897557321772	24.526137727072996
170-174	22.523228096377533	29.308996860421964	25.8305083202233	22.337266722977205
175-179	20.054185610760626	29.272341888436838	28.538604970960613	22.134867529841927
180-184	20.11697717146558	30.312295807900625	26.752164578547504	22.818562442086282
185-189	21.915210902691296	27.852405681748326	27.71311678820484	22.519266627355538
190-194	20.079092778059838	27.480118661934156	26.80329940281993	25.63748915718608
195-199	20.95243732796638	30.596656429077186	27.375253568168002	21.075652674788437
200-204	22.28872734392609	27.681834843169085	26.90320127677691	23.126236536127916
205-209	20.994920668167776	27.8577787086977	28.731350929237692	22.415949693896835
210-214	23.54428535326514	26.986073387351983	27.74034619596522	21.729295063417656
215-219	23.078425703103832	26.692332736077372	28.10088541109348	22.128356149725313
220-224	18.716484036873535	27.028101386286487	28.849466453870516	25.405948122969463
225-229	21.656303858334226	28.6494350353231	26.452276820169796	23.24198428617288
230-234	21.00908178156824	27.084062827988003	28.363526253839094	23.543329136604658
235-239	22.437350733440944	26.380879309520505	25.654200181681162	25.527569775357385
240-244	22.594625607026828	30.76982271287773	23.647055877203567	22.988495802891872
245-249	20.908132622697394	28.90925276221396	24.60472962973925	25.577884985349396
250-254	20.861278006333798	26.008774426213087	26.867730192085716	26.262217375367403
255-259	21.395211175896055	27.15596121403089	27.43850047013986	24.010327139933203
260-264	21.029116797311666	28.35505690627483	26.625579706350415	23.990246590063087
265-269	20.56425889793753	27.667901400402066	26.921050654439238	24.846789047221165
270-274	18.841793953523318	29.688068465112888	25.583394614678834	25.886742966684956
275-279	20.305852728974045	28.079302235042608	24.632733117653352	26.98211191832999
280-284	17.46948644335213	29.458121125330404	26.907481484685736	26.164910946631725
285-289	20.250619833299563	26.547898486217957	28.81367663029249	24.387805050189993
290-294	19.89290373402606	29.94815939676389	25.68152065769493	24.477416211515123
295-299	19.132984693703314	30.516926173243693	23.423301178650867	26.926787954402126
300-301	14.87497467164203	32.69054324489847	26.89541494111299	25.53906714234651
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.25
28	0.25
29	0.5
30	0.5
31	1.75
32	4.25
33	8.25
34	21.0
35	58.0
36	198.5
37	905.0
38	3267.5
39	4896.0
40	7706.5
41	9235.0
42	12374.5
43	19434.75
44	19600.0
45	15881.5
46	12514.5
47	10003.75
48	8559.75
49	6168.5
50	5339.5
51	3830.0
52	992.0
53	350.75
54	282.0
55	303.5
56	379.25
57	387.5
58	559.75
59	919.5
60	991.75
61	736.0
62	312.0
63	82.25
64	20.0
65	8.5
66	6.5
67	2.75
68	2.75
69	4.0
70	3.25
71	3.0
72	3.0
73	2.75
74	2.5
75	1.75
76	1.5
77	0.5
78	0.25
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.2767063344800344E-4
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	439231.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.215312261627142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	42.4067614908615	3.9079154914263365
2	6.268641902015654	1.1553498516678906
3	3.431938782549336	0.9487916263194186
4	4.15078258403151	1.5300303056789586
5	3.3040646395125552	1.5223993692854356
6	3.8203897621304197	2.112365077149315
7	3.56483893871249	2.299577278785016
8	3.0644557961458534	2.2591933658749794
9	3.1193695723014994	2.5871368201358953
>10	25.086717209937632	42.49071124821224
>50	1.385909601428961	8.727388354723521
>100	0.32453861337936063	4.469473020005519
>500	0.01728061203517913	1.120411017373787
>1k	0.03949854178767536	9.943334972587255
>5k	0.00740597658518913	4.843815508289738
>10k+	0.00740597658518913	10.082106692484716
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	21265	4.841416020271793	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	12667	2.8839039138858595	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	10386	2.364587198990964	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	8938	2.0349201217582547	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	7093	1.6148678030466885	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	5261	1.1977752025699462	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	4994	1.1369871434393293	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4921	1.120367187197625	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	4373	0.9956036800681192	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	4255	0.9687385453212547	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	3756	0.855130899230701	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	3136	0.7139751064929388	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	2955	0.6727667218388501	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2836	0.6456739164585378	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2613	0.5949033651996329	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2518	0.5732746550220726	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	1360	0.3096320614892847	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1289	0.2934674465144764	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1265	0.2880033513117244	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1209	0.2752537958386362	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	1159	0.263870264166236	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT	1069	0.2433799071559157	TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	819	0.18646224879391482	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	789	0.17963212979047471	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	764	0.17394036395427462	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	727	0.1655165505166985	No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT	641	0.1459368760401702	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	596	0.13569169753501004	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	589	0.13409800310087402	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	2.2767063344800344E-4	0.0	0.0
7	2.2767063344800344E-4	0.0	0.0
8	4.5534126689600687E-4	0.0	0.0
9	4.5534126689600687E-4	0.0	0.0
10-14	6.830119003440104E-4	0.0	0.0
15-19	6.830119003440104E-4	0.0	0.0
20-24	7.740801537232117E-4	0.0	0.0
25-29	9.106825337920137E-4	0.0	0.0
30-34	0.003187388868272048	0.0	0.0
35-39	0.003187388868272048	0.0	0.0
40-44	0.005145356315924878	0.0	0.0
45-49	0.008059540424059323	0.0	0.0
50-54	0.010472849138608159	0.0	0.0
55-59	0.012248680079502585	0.0	0.0
60-64	0.014844125300809825	0.0	0.0
65-69	0.021537641924181127	0.0	0.0
70-74	0.03114534265568687	0.0	0.0
75-79	0.04334848860849986	0.0	0.0
80-84	0.05869348930289529	0.0	0.0
85-89	0.08050433598721402	0.0	0.0
90-94	0.12016456033385622	0.0	0.0
95-99	0.15909623865346478	0.0	0.0
100-104	0.20071443044775986	0.0	0.0
105-109	0.24843419521846136	0.0	0.0
110-114	0.31081594878321434	0.0	0.0
115-119	0.3842169610068506	0.0	0.0
120-124	0.4652221723876503	0.0	0.0
125-129	0.5584760638479523	0.0	0.0
130-134	0.6716739027982997	0.0	0.0
135-139	0.797894501981873	0.0	0.0
140-144	0.9595861858566448	0.0	0.0
145-149	1.1718207503568736	0.0	0.0
150-154	1.3938451520953667	0.0	0.0
155-159	1.6708747788748972	0.0	0.0
160-164	1.9494525659618744	0.0	0.0
165-169	2.276797402733414	0.0	0.0
170-174	2.654958324890547	0.0	0.0
175-179	3.0860754363876866	0.0	0.0
180-184	3.5828982926979203	0.0	0.0
185-189	4.226341036948667	0.0	0.0
190-194	4.992725923261337	0.0	0.0
195-199	5.730651980393005	0.0	0.0
200-204	6.526770651433983	0.0	0.0
205-209	7.418556522649813	0.0	0.0
210-214	8.387978079871411	0.0	0.0
215-219	9.389000321015594	0.0	0.0
220-224	10.492064540071171	4.5534126689600686E-5	0.0
225-229	11.66998686340445	2.2767063344800344E-4	0.0
230-234	12.901138580837872	2.2767063344800344E-4	0.0
235-239	14.131425149864194	2.2767063344800344E-4	0.0
240-244	15.38966967267793	2.2767063344800344E-4	0.0
245-249	16.52000883362058	2.2767063344800344E-4	0.0
250-254	17.781440745302586	2.2767063344800344E-4	0.0
255-259	19.045149363319073	2.2767063344800344E-4	0.0
260-264	20.316963055886312	2.2767063344800344E-4	0.0
265-269	21.4300447828136	2.2767063344800344E-4	0.0
270-274	22.491900617215087	2.2767063344800344E-4	1.3660238006880208E-4
275-279	23.471612887068535	2.2767063344800344E-4	2.2767063344800344E-4
280-284	24.402512573110734	2.2767063344800344E-4	2.2767063344800344E-4
285-289	25.272988473035827	2.2767063344800344E-4	2.2767063344800344E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	7000	0.0	292.68213	1
GGGTTAT	7085	0.0	289.37897	2
GGGGGGA	5560	0.0	285.44962	1
GTTATTA	7210	0.0	284.77115	4
GGTTATT	7225	0.0	283.9758	3
TTAGTAG	7470	0.0	276.04416	8
TTATTAG	7460	0.0	275.62335	5
TAGTAGA	7535	0.0	273.6629	9
TATTAGT	7660	0.0	268.812	6
GGGGAGC	5950	0.0	266.9874	3
CGAAAGC	4660	0.0	261.765	9
GGGAGCG	4655	0.0	261.0956	4
GCGAAAG	4690	0.0	260.40512	8
GGAGCGA	4680	0.0	260.01602	5
ATTAGTA	7955	0.0	258.84348	7
AGCGAAA	4935	0.0	247.4772	7
GAGCGAA	5010	0.0	243.18362	6
GGGGGAG	6695	0.0	237.27783	2
AGACCCC	190	0.0	217.36844	295
AAACGTT	225	0.0	216.33331	295
>>END_MODULE