##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AJTU1_l01n01_6617_flu.3510000003bae3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 453311 Sequences flagged as poor quality 0 Sequence length 301 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.79196180988328 34.0 34.0 34.0 34.0 34.0 2 33.86981785132062 34.0 34.0 34.0 34.0 34.0 3 33.880788244715 34.0 34.0 34.0 34.0 34.0 4 33.89612870634068 34.0 34.0 34.0 34.0 34.0 5 33.879978646006826 34.0 34.0 34.0 34.0 34.0 6 37.63935576237947 38.0 38.0 38.0 38.0 38.0 7 37.663206937400595 38.0 38.0 38.0 38.0 38.0 8 37.65772504968995 38.0 38.0 38.0 38.0 38.0 9 37.56825887745941 38.0 38.0 38.0 37.0 38.0 10-14 37.656622054174726 38.0 38.0 38.0 37.8 38.0 15-19 37.62481960508349 38.0 38.0 38.0 37.2 38.0 20-24 37.61561312211705 38.0 38.0 38.0 37.0 38.0 25-29 37.52672006635621 38.0 38.0 38.0 37.0 38.0 30-34 37.470399791754446 38.0 38.0 38.0 37.0 38.0 35-39 37.37929942136856 38.0 38.0 38.0 37.0 38.0 40-44 37.39373057349149 38.0 38.0 38.0 37.0 38.0 45-49 37.36064467881873 38.0 38.0 38.0 37.0 38.0 50-54 37.323405344233876 38.0 38.0 38.0 37.0 38.0 55-59 37.26907222635233 38.0 38.0 38.0 37.0 38.0 60-64 37.10188854892116 38.0 38.0 38.0 36.4 38.0 65-69 37.031168447269096 38.0 38.0 38.0 36.4 38.0 70-74 37.11241818530766 38.0 38.0 38.0 37.0 38.0 75-79 37.04878240325075 38.0 38.0 38.0 36.4 38.0 80-84 36.93956025774799 38.0 38.0 38.0 36.0 38.0 85-89 36.974164315447894 38.0 38.0 38.0 36.0 38.0 90-94 36.825623027016775 38.0 38.0 38.0 35.8 38.0 95-99 36.83363298044831 38.0 38.0 38.0 35.8 38.0 100-104 36.80061238311005 38.0 38.0 38.0 35.6 38.0 105-109 36.65314938309461 38.0 38.0 38.0 35.0 38.0 110-114 36.56724500398181 38.0 37.8 38.0 34.4 38.0 115-119 36.64628433900788 38.0 38.0 38.0 34.8 38.0 120-124 36.54429034371547 38.0 37.4 38.0 34.2 38.0 125-129 36.45797388547818 38.0 37.0 38.0 34.0 38.0 130-134 36.31745755121759 38.0 37.0 38.0 34.0 38.0 135-139 36.07102232242324 38.0 37.0 38.0 33.0 38.0 140-144 36.02684867563328 38.0 37.0 38.0 32.4 38.0 145-149 35.596699837418456 38.0 36.4 38.0 29.8 38.0 150-154 35.22128163666886 38.0 36.2 38.0 28.0 38.0 155-159 35.28390266285178 38.0 36.0 38.0 28.6 38.0 160-164 35.25823683960901 38.0 35.8 38.0 28.0 38.0 165-169 35.01809397962988 38.0 35.2 38.0 27.6 38.0 170-174 34.91156270198606 38.0 35.0 38.0 27.2 38.0 175-179 34.5256832505719 38.0 35.0 38.0 24.4 38.0 180-184 34.24768381971759 37.6 34.6 38.0 23.4 38.0 185-189 33.48339969689683 37.0 33.4 37.8 22.2 38.0 190-194 32.507720306809226 36.8 30.6 37.0 20.6 38.0 195-199 32.24054082076102 36.8 29.8 37.2 20.2 37.8 200-204 32.05784108481815 36.8 29.8 37.0 19.8 37.4 205-209 30.97896852271399 35.4 27.4 37.0 17.8 37.2 210-214 30.20317133270536 34.2 25.4 37.0 15.8 37.0 215-219 29.340341619770975 33.0 24.0 36.8 14.0 37.0 220-224 27.843312427891668 31.2 21.4 36.2 9.4 37.0 225-229 26.78993626891913 29.4 20.0 36.0 9.0 37.0 230-234 26.356047834709507 28.8 19.6 35.6 9.0 37.0 235-239 25.291496125176756 27.4 18.0 34.2 8.8 37.0 240-244 24.219073439647396 25.8 16.6 33.0 8.0 37.0 245-249 23.346341474175567 24.4 15.0 32.0 8.0 36.2 250-254 22.43607104173514 22.8 13.4 31.4 8.0 36.0 255-259 21.799037305514318 21.8 13.0 30.4 8.0 36.0 260-264 20.52712949829146 20.2 11.4 29.2 7.8 34.8 265-269 19.697548040969668 19.0 9.8 27.6 7.0 33.8 270-274 18.742589083432787 18.2 8.4 26.6 7.0 33.0 275-279 17.518699524167733 16.0 8.0 24.6 7.0 31.2 280-284 17.266160759390353 16.2 8.0 24.0 7.0 30.6 285-289 16.14543657665488 13.6 8.0 21.6 7.0 29.4 290-294 15.498342197740623 13.0 8.0 20.8 7.0 28.4 295-299 14.705824478117671 12.8 8.0 19.2 7.0 27.0 300-301 12.977151447902212 10.0 8.0 16.0 7.0 22.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.04392301071867166 1101 2 8.0722174261183E-4 1101 3 0.010460615401406415 1101 4 0.002550705030593292 1101 5 0.009848826363246133 1101 6 0.08034371197144452 1101 7 0.01967037666864968 1101 8 0.006187870948437535 1101 9 0.04568232544927042 1101 10-14 0.05166712227865844 1101 15-19 0.044139194191856745 1101 20-24 0.06257933396928195 1101 25-29 0.028132689318319137 1101 30-34 0.08054861822657955 1101 35-39 0.09335164964373632 1101 40-44 0.0703340586799115 1101 45-49 0.13452101164119057 1101 50-54 0.1311621701700858 1101 55-59 0.14262767975115764 1101 60-64 0.25863942097146264 1101 65-69 0.25984722792762227 1101 70-74 0.220519994321549 1101 75-79 0.07852313809851097 1101 80-84 0.15988148512792577 1101 85-89 0.13228417859139796 1101 90-94 0.3022357936254636 1101 95-99 0.16109955107012297 1101 100-104 0.20680054107694446 1101 105-109 0.1526674722991217 1101 110-114 0.18309784473103718 1101 115-119 0.14677947235496447 1101 120-124 0.09660055658071798 1101 125-129 0.16932512774886277 1101 130-134 0.17606202167596052 1101 135-139 0.16806663909444097 1101 140-144 0.15437106003522416 1101 145-149 0.45430154092577624 1101 150-154 0.7013241865213757 1101 155-159 0.3926685892200936 1101 160-164 0.40119071015833185 1101 165-169 0.3123498860421705 1101 170-174 0.3832720084565153 1101 175-179 0.44514010709553986 1101 180-184 0.2546319693225243 1101 185-189 0.41350340057284285 1101 190-194 0.3913384606483632 1101 195-199 0.41863754968353106 1101 200-204 -0.05581020709864504 1101 205-209 -0.2489128561189844 1101 210-214 -0.19805228949963904 1101 215-219 -0.06740621232773591 1101 220-224 0.34531120733191045 1101 225-229 1.0932631980799208 1101 230-234 0.7040736612627114 1101 235-239 0.5804216327327438 1101 240-244 0.5452107894094098 1101 245-249 0.4734985786577077 1101 250-254 0.3311816653372368 1101 255-259 0.19197327361538186 1101 260-264 0.2794421967070235 1101 265-269 0.10689823416593569 1101 270-274 -0.028665431360590077 1101 275-279 0.38311285158269115 1101 280-284 0.2698672127087569 1101 285-289 0.4096652939453662 1101 290-294 0.24742018782433917 1101 295-299 0.09800055165029242 1101 300-301 0.18114168508151707 1102 1 -0.029377159745692438 1102 2 0.018345241620153274 1102 3 -0.026623527993038465 1102 4 -0.029380541221925682 1102 5 -0.015300073467777509 1102 6 0.014485471151630236 1102 7 0.11254317133406033 1102 8 -0.019702348358578092 1102 9 0.11011825037250134 1102 10-14 0.09202949668133442 1102 15-19 0.06709320694691456 1102 20-24 0.034334625863820634 1102 25-29 -0.008051405018221658 1102 30-34 0.04812548469823241 1102 35-39 0.046506654458674745 1102 40-44 -0.006817094180895822 1102 45-49 0.07627990111554084 1102 50-54 0.02824318839418538 1102 55-59 0.011871424868083125 1102 60-64 0.1991233863038815 1102 65-69 0.23335290592605418 1102 70-74 0.1364420104198132 1102 75-79 0.007553281456608829 1102 80-84 0.1851674563836312 1102 85-89 0.09951640418509555 1102 90-94 0.21508932852017182 1102 95-99 0.20105957796112506 1102 100-104 0.19846534838838892 1102 105-109 0.1663629862261189 1102 110-114 0.3660270014801341 1102 115-119 0.1355227541472246 1102 120-124 0.15055713124866799 1102 125-129 0.0024459737252087166 1102 130-134 0.24521942487744042 1102 135-139 0.18547732645021142 1102 140-144 0.27339833259731705 1102 145-149 0.462379016078863 1102 150-154 0.7979092474803764 1102 155-159 0.5501428701583819 1102 160-164 0.5905899870084497 1102 165-169 0.6419424147047081 1102 170-174 0.3764539042720614 1102 175-179 0.6408887125791196 1102 180-184 0.7267004323564876 1102 185-189 0.8280223605452264 1102 190-194 1.0824441531824718 1102 195-199 0.8477674804573709 1102 200-204 0.5051453682415783 1102 205-209 0.32074886236130595 1102 210-214 -0.1896165099331597 1102 215-219 0.13597144270433503 1102 220-224 0.9504662848252394 1102 225-229 1.5380625875815248 1102 230-234 1.0435225411071727 1102 235-239 1.214517095784867 1102 240-244 1.3153145377968585 1102 245-249 1.110713107065866 1102 250-254 1.0799361396000045 1102 255-259 0.4581848767187502 1102 260-264 0.9798912764170353 1102 265-269 0.9255262843866952 1102 270-274 1.129700322586757 1102 275-279 1.4251266241357037 1102 280-284 0.3899028251149552 1102 285-289 1.2483947305062273 1102 290-294 0.9752216304901875 1102 295-299 0.7698607184471733 1102 300-301 0.584321000814569 1103 1 -0.06020614104436106 1103 2 0.03301381905559708 1103 3 0.01446849174971021 1103 4 0.026712971746050584 1103 5 0.07878220848811424 1103 6 -0.022948671320939695 1103 7 0.05024245745349987 1103 8 0.1152383527648766 1103 9 0.15442402653139453 1103 10-14 0.0730103533240225 1103 15-19 -0.035476049678706545 1103 20-24 0.040677091301084545 1103 25-29 0.01271344802673724 1103 30-34 0.017261253492407036 1103 35-39 -0.4674688846236066 1103 40-44 -0.277367825021976 1103 45-49 -0.1567124732944194 1103 50-54 0.017614641984252444 1103 55-59 -0.40837223312108506 1103 60-64 0.15124603919510804 1103 65-69 0.21601904384412052 1103 70-74 0.05453815174396226 1103 75-79 0.2147357298004593 1103 80-84 0.18583272844086451 1103 85-89 0.22357593456401048 1103 90-94 0.27918736802384814 1103 95-99 0.2722201881482107 1103 100-104 0.27375047747369763 1103 105-109 -0.004114377248683354 1103 110-114 0.2638386396845931 1103 115-119 0.043576029066421995 1103 120-124 0.3102727819976252 1103 125-129 0.2722556172325383 1103 130-134 0.437316591526276 1103 135-139 0.38382337842563885 1103 140-144 0.42088967923469767 1103 145-149 0.7372046374458989 1103 150-154 0.8939837087128168 1103 155-159 -0.03618268371628375 1103 160-164 -0.4918030340694628 1103 165-169 0.49567684162445147 1103 170-174 0.7950787242632416 1103 175-179 0.48826165030218505 1103 180-184 0.6384894980311131 1103 185-189 0.5178280734081611 1103 190-194 0.3885744897141805 1103 195-199 0.4990812738719299 1103 200-204 0.6088021964073675 1103 205-209 0.9554691816247534 1103 210-214 0.8921193521613624 1103 215-219 0.6272824105310981 1103 220-224 1.146031231030662 1103 225-229 1.985817679059931 1103 230-234 1.0863108926914329 1103 235-239 1.251063276310557 1103 240-244 1.2915644932099184 1103 245-249 1.3311976394205942 1103 250-254 1.4111963027986825 1103 255-259 0.5554961620744443 1103 260-264 1.5250625636890902 1103 265-269 0.38990016840191544 1103 270-274 0.854600395394602 1103 275-279 1.6639658670740083 1103 280-284 0.5066734580256416 1103 285-289 0.606430275561415 1103 290-294 1.1558246696756331 1103 295-299 0.9235493344756716 1103 300-301 -0.7637102848768365 2116 1 1.4398180576336017E-4 2116 2 0.03884919989097568 2116 3 0.044208458989672295 2116 4 0.05059196437504454 2116 5 -0.011256694050786109 2116 6 -0.15485899073125609 2116 7 -0.097894315683277 2116 8 0.08723876226527949 2116 9 0.106896385253755 2116 10-14 0.0947036375173056 2116 15-19 0.0968181763655167 2116 20-24 -0.11759993572594851 2116 25-29 -0.1505953152820254 2116 30-34 0.07427906676021934 2116 35-39 -0.07098546939019457 2116 40-44 -0.15481458996873698 2116 45-49 0.04710611802416764 2116 50-54 0.19464678776639488 2116 55-59 0.12844697244810277 2116 60-64 0.32030316450223495 2116 65-69 0.27484377766884904 2116 70-74 -0.8058529211471139 2116 75-79 0.24447569704042849 2116 80-84 -0.04596497835684232 2116 85-89 0.14565824414631123 2116 90-94 0.2775391288756026 2116 95-99 0.2927003273783484 2116 100-104 0.2575700925433111 2116 105-109 0.31306928368497466 2116 110-114 0.4305872064331524 2116 115-119 0.444210754701146 2116 120-124 -0.12756505584021482 2116 125-129 0.07336638709331567 2116 130-134 0.27972547768515454 2116 135-139 0.48240548325775023 2116 140-144 0.5537316120766249 2116 145-149 0.8183369060781658 2116 150-154 1.0659124556415662 2116 155-159 -0.25336634464994034 2116 160-164 -0.4059205606869867 2116 165-169 0.5427182011658118 2116 170-174 0.8902517381862509 2116 175-179 0.7718304463709842 2116 180-184 0.8771975242391363 2116 185-189 1.164598138928227 2116 190-194 0.9370388631785573 2116 195-199 0.9871340994247575 2116 200-204 0.8663370039421778 2116 205-209 0.9138690587746332 2116 210-214 0.7615962153882307 2116 215-219 1.0094947364934228 2116 220-224 1.2594730819725157 2116 225-229 1.0532419214841724 2116 230-234 1.1716303030108364 2116 235-239 1.10117077016805 2116 240-244 1.6383857462811733 2116 245-249 1.1051278196007779 2116 250-254 1.315572838425215 2116 255-259 0.563256194834473 2116 260-264 0.45270384133036856 2116 265-269 0.9135027482544942 2116 270-274 1.208408749202956 2116 275-279 1.888479585337727 2116 280-284 0.5073133024154828 2116 285-289 -1.0003367187055794 2116 290-294 0.619358658209622 2116 295-299 0.7977610461873805 2116 300-301 1.3345186258520734 1117 1 -0.057220883059102334 1117 2 0.0340519025936743 1117 3 0.0010102607914745931 1117 4 0.032089712122790104 1117 5 0.051355918561824865 1117 6 -0.30215628802855576 1117 7 -0.2046735949625571 1117 8 0.015549573076093282 1117 9 0.13206530417267714 1117 10-14 0.09008201589567477 1117 15-19 0.07901862681596583 1117 20-24 0.05638429850829141 1117 25-29 0.10869297300626357 1117 30-34 0.04408762531877386 1117 35-39 -0.17194267659739637 1117 40-44 -0.08049927465342677 1117 45-49 0.06310611802416588 1117 50-54 0.10336075173036363 1117 55-59 0.21735463010576694 1117 60-64 0.21045856990763667 1117 65-69 0.22205999388507536 1117 70-74 -0.13848355177774607 1117 75-79 0.1954486700134055 1117 80-84 -0.017086599978462402 1117 85-89 0.06405013603820464 1117 90-94 0.2798634531999298 1117 95-99 0.2556350120630313 1117 100-104 0.15276153398475145 1117 105-109 0.18480577017145805 1117 110-114 0.24092053976649197 1117 115-119 0.2528503943407898 1117 120-124 0.33614665587149517 1117 125-129 0.3516974681743932 1117 130-134 0.2053989011085804 1117 135-139 0.3430879156901838 1117 140-144 0.4078036841486963 1117 145-149 0.8241206898619495 1117 150-154 0.9587142574433614 1117 155-159 -0.059427155460753056 1117 160-164 -0.665614254380678 1117 165-169 0.22763712008472226 1117 170-174 0.75777200845652 1117 175-179 0.8764025184430579 1117 180-184 0.765922749464373 1117 185-189 1.0295778686579453 1117 190-194 0.9934803046199931 1117 195-199 0.7786836489743081 1117 200-204 0.8289451120502846 1117 205-209 1.432119058774628 1117 210-214 0.749039909081926 1117 215-219 0.5396186103672953 1117 220-224 1.1346622711617051 1117 225-229 0.7912419214841719 1117 230-234 1.1384140867946222 1117 235-239 0.8661414908887721 1117 240-244 0.9740051156505452 1117 245-249 1.5134453871683426 1117 250-254 1.5683093249117022 1117 255-259 1.0625264651047424 1117 260-264 0.5044741116006399 1117 265-269 0.990369865371612 1117 270-274 1.4410934338876409 1117 275-279 1.1247476033557469 1117 280-284 0.19144168079386148 1117 285-289 -0.5970439259127893 1117 290-294 0.6798811807321474 1117 295-299 0.01643672186305878 1117 300-301 0.13998033756378447 2117 1 -0.013132935176365379 2117 2 0.019646365134391885 2117 3 0.06228333353848825 2117 4 0.019804152947983766 2117 5 -0.012989901199304654 2117 6 -0.6168549850969569 2117 7 -0.024703453811632414 2117 8 -0.028456941581886497 2117 9 0.11529005987299712 2117 10-14 0.06554944260578566 2117 15-19 0.09601319793431884 2117 20-24 0.07649450480578679 2117 25-29 0.15636832588356953 2117 30-34 0.1113460400853441 2117 35-39 0.0943575405578656 2117 40-44 0.1752265668232269 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-0.9632425166420688 2110 185-189 -1.8790229716781823 2110 190-194 -0.7172479866965311 2110 195-199 -2.7638555667119675 2110 200-204 -1.4411011064371095 2110 205-209 -1.2693977479480587 2110 210-214 -1.1396435643074305 2110 215-219 -1.2078953952349423 2110 220-224 -0.8014007540483838 2110 225-229 -0.485035389440192 2110 230-234 -0.9664766695078981 2110 235-239 -1.1993445035089891 2110 240-244 -0.5239220552178026 2110 245-249 -0.9506344447644288 2110 250-254 -0.4391276498782126 2110 255-259 -0.6051710138868565 2110 260-264 -0.7692107623489406 2110 265-269 -0.5284802746844157 2110 270-274 -0.8432651095297246 2110 275-279 -0.348940071714285 2110 280-284 0.12960974802075143 2110 285-289 -0.646024318069653 2110 290-294 -0.5980487912566517 2110 295-299 -0.40469353023778254 2110 300-301 -0.5523340882065213 2109 1 -0.24019556660340413 2109 2 -0.027751894874675997 2109 3 -0.07387370545325211 2109 4 0.006752159380177147 2109 5 0.018391783540728568 2109 6 -0.5130740095475446 2109 7 -0.26799637977268276 2109 8 -0.1319609754471145 2109 9 -0.34346212198766324 2109 10-14 -0.029481275243554705 2109 15-19 -0.10277884153845918 2109 20-24 -0.04781190402335511 2109 25-29 -0.06652643627643329 2109 30-34 -0.2105748219386001 2109 35-39 -0.16391525043705713 2109 40-44 -0.12784315650996803 2109 45-49 -0.14007742627963182 2109 50-54 -0.13306118919790322 2109 55-59 -0.09299347116004952 2109 60-64 -0.14842117692780477 2109 65-69 -3.100606672781602 2109 70-74 -0.027042623507696817 2109 75-79 -0.3150935240794297 2109 80-84 -0.30629968014724085 2109 85-89 -0.3227979652276076 2109 90-94 -0.2562272640996426 2109 95-99 -0.20091140143907182 2109 100-104 -0.46940091327263644 2109 105-109 -0.4285085758200964 2109 110-114 -0.6593705994740162 2109 115-119 -0.42068547063811934 2109 120-124 -0.5466550318922074 2109 125-129 -0.27224556980029035 2109 130-134 -0.2937762043766483 2109 135-139 -0.32718212650391365 2109 140-144 -0.3865169909567854 2109 145-149 -0.4701261455810908 2109 150-154 -0.2484249830629679 2109 155-159 -0.31450943394176534 2109 160-164 -0.5132956889798379 2109 165-169 -0.5966645676789781 2109 170-174 -1.0462976117966392 2109 175-179 -1.1345679456919697 2109 180-184 -0.6572502463162166 2109 185-189 -1.6540107389369822 2109 190-194 -1.318038682721781 2109 195-199 -1.619656013472948 2109 200-204 -0.955048558835788 2109 205-209 -0.9403767218160866 2109 210-214 -1.1165297111712391 2109 215-219 -1.134906706088401 2109 220-224 -1.1139221170239537 2109 225-229 -0.7882981629040131 2109 230-234 -1.2785732549775268 2109 235-239 -0.6211095639635502 2109 240-244 -0.9847290615646429 2109 245-249 -0.43531621620718397 2109 250-254 -0.8590113501937822 2109 255-259 -0.7267899905914561 2109 260-264 -0.3471102765850169 2109 265-269 -1.0739740164849323 2109 270-274 -0.8064846251840905 2109 275-279 -0.30591273419700116 2109 280-284 -0.2648199225816654 2109 285-289 -0.5295586938452779 2109 290-294 -0.21919476863494758 2109 295-299 0.01270887376179175 2109 300-301 -0.7201726160227118 2108 1 0.018270920219585207 2108 2 -0.025251376094850286 2108 3 -0.06430394685880003 2108 4 -0.07334744634715662 2108 5 -0.04830255138352868 2108 6 -0.017525140487570923 2108 7 0.04263514820684122 2108 8 -0.01608977118620203 2108 9 0.05747514023824607 2108 10-14 0.0031229995022457047 2108 15-19 -0.04745131853921691 2108 20-24 -0.08714302389607553 2108 25-29 -0.0815966444800722 2108 30-34 -0.1611855987249342 2108 35-39 -0.09048092796351881 2108 40-44 -0.040715121647963315 2108 45-49 -0.17801683279549962 2108 50-54 -0.2519288657559784 2108 55-59 -0.06880657208002106 2108 60-64 -0.0867299546825322 2108 65-69 -0.18918590775427901 2108 70-74 -0.28745349713292967 2108 75-79 -0.2043955922816778 2108 80-84 -0.15766856719157119 2108 85-89 -0.12658920822408248 2108 90-94 0.05485525647862488 2108 95-99 -0.025231108155544746 2108 100-104 -0.26815376656170287 2108 105-109 -0.25702209868099857 2108 110-114 -0.2731832853701164 2108 115-119 -0.3201113542930898 2108 120-124 -0.13639979221592569 2108 125-129 -0.24958941707151183 2108 130-134 -0.37534153604988774 2108 135-139 -0.29497765808030607 2108 140-144 -0.3538138021901034 2108 145-149 0.03388298494391506 2108 150-154 0.283588574383252 2108 155-159 -0.24333699152632704 2108 160-164 -0.38511971886155294 2108 165-169 -0.07602681434151037 2108 170-174 -0.6072853685926631 2108 175-179 -0.6287887383875415 2108 180-184 -0.3396400920656859 2108 185-189 -0.8597090165879564 2108 190-194 -0.21657434018874966 2108 195-199 -1.3119365696049279 2108 200-204 -0.8506368551628256 2108 205-209 -0.3990202854876621 2108 210-214 0.1317175047103376 2108 215-219 -0.6876901328020999 2108 220-224 0.0909163695223576 2108 225-229 0.14807798705794184 2108 230-234 -0.11417607713979905 2108 235-239 -0.2193858315156021 2108 240-244 -0.08053040347514084 2108 245-249 0.21501096093883731 2108 250-254 -0.06685460951452882 2108 255-259 -0.48262107587886405 2108 260-264 0.6701872263547379 2108 265-269 -0.5072831400928699 2108 270-274 -0.5551579322325786 2108 275-279 -0.06920594855682438 2108 280-284 -0.2595747126487602 2108 285-289 0.08063913419650248 2108 290-294 -0.3548128083388953 2108 295-299 0.20814983661715658 2108 300-301 -0.42952102855643304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 20.0 14 86.0 15 295.0 16 405.0 17 617.0 18 1115.0 19 1125.0 20 1283.0 21 1721.0 22 2471.0 23 3821.0 24 5452.0 25 7536.0 26 10649.0 27 14558.0 28 20467.0 29 28669.0 30 40917.0 31 56904.0 32 73448.0 33 83666.0 34 68021.0 35 27701.0 36 2362.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.14239230903287 21.1724401128585 15.69805277171743 30.987114806391197 2 44.89434405959705 23.5683669710199 16.557286278073992 14.980002691309057 3 44.786029900002426 22.347130336568053 18.44473220371886 14.422107559710662 4 45.305981985877246 22.048218551943368 18.358919152634726 14.286880309544662 5 29.62513594419725 22.334776786797587 33.70952833705778 14.330558931947381 6 28.03991078972273 22.691264937316767 34.29014517626972 14.978679096690792 7 17.077238363948812 47.68293732117685 20.242614893527843 14.997209421346493 8 27.703938355786644 20.6721213471546 35.56741398289475 16.056526314164007 9 15.4489963843807 19.149766937047634 37.26051209875781 28.14072457981386 10-14 26.42040453463516 34.61327874240863 22.990970878712407 15.975345844243796 15-19 21.950625508756684 35.339843948194506 21.23186951121857 21.477661031830245 20-24 25.292304841488516 30.488472593870437 26.35078345771446 17.868439106926594 25-29 19.79100440977607 25.934027632243644 36.509460392534045 17.76550756544624 30-34 19.647835591900485 28.056720441374683 30.927553048569305 21.367890918155528 35-39 21.075950065187037 29.942225094912768 27.802943233232813 21.17888160666739 40-44 20.911647853239828 29.199291435681022 28.26236292523235 21.6266977858468 45-49 21.545076117720505 29.957975870870108 26.33816518902034 22.158782822389046 50-54 22.243581117599177 29.238558076022862 27.536415396934995 20.981445409442966 55-59 22.03480612647829 26.914414165992 29.481967126321663 21.568812581208043 60-64 21.871165711840217 26.46990697335825 28.582099265184386 23.076828049617152 65-69 20.594161624138838 27.776162502123263 28.347646538469174 23.28202933526872 70-74 21.118966890280625 26.3810055348315 29.259514990812047 23.240512584075834 75-79 20.480111887865064 25.609879310230728 30.39379145884393 23.516217343060283 80-84 23.879764664876873 25.937910176457223 27.12857177522716 23.053753383438742 85-89 21.417658075802265 28.898614858231987 25.844111437842894 23.839615628122857 90-94 20.451257525186904 30.377246526115627 28.615542089205864 20.55595385949161 95-99 22.201137850173502 29.81489529263574 26.631120797862835 21.352846059327923 100-104 22.751841451012663 28.728268230861374 26.149332356814636 22.37055796131133 105-109 23.67853416308009 28.14650427631361 24.163146272647257 24.011815287959042 110-114 21.48780859057027 28.44757793214813 29.02995956418441 21.03465391309719 115-119 23.765494329500054 27.596550712424804 26.646430375614095 21.991524582461047 120-124 19.671042617540717 28.26073049186982 27.788383692431907 24.279843198157554 125-129 21.088568333881156 25.126326076358175 30.962407706850264 22.822697882910408 130-134 24.844312183026666 28.386207261681275 25.986530218768127 20.782950336523932 135-139 24.177220495421466 27.295212337666637 25.20084445336645 23.326722713545447 140-144 21.215192219028438 29.460216054761524 26.663019428163004 22.661572298047037 145-149 20.916501033506798 27.443587294374062 28.900512010518163 22.739399661600977 150-154 22.99639761664729 25.99650129822572 26.06021031918484 24.946890765942147 155-159 21.912638343212496 26.94926882427296 28.95601474484405 22.182078087670494 160-164 24.602667925552215 29.459863096196653 25.923085916732663 20.014383061518473 165-169 20.627957406725184 26.2152473688042 28.269775055094627 24.887020169375994 170-174 21.935580649929076 29.095477497788497 26.360401578607178 22.60854027367525 175-179 20.578013769796012 28.82365528301762 28.453710587212754 22.144620359973615 180-184 20.21208397766655 29.53129308576231 26.627017654546215 23.629605282024922 185-189 21.833355025578467 27.578593945437017 27.663921678494457 22.92412935049006 190-194 19.89292119538242 28.097487155617223 27.22056160119653 24.789030047803827 195-199 20.70084335037094 30.203370313096308 27.248886526027384 21.84689981050537 200-204 22.30790781604682 27.561475455040803 27.081804765381822 23.048811963530554 205-209 21.058478616225944 27.610624935199013 27.635464394201776 23.695432054373267 210-214 22.706530395247412 27.166558940771345 27.549386624193982 22.577524039787253 215-219 22.652704214104666 26.827542239213255 28.020498068654852 22.499255478027226 220-224 18.83713388821361 27.279990999556595 28.40672297826437 25.476152133965424 225-229 21.316844285711134 28.576760766890725 26.026149817674842 24.080245129723302 230-234 20.998916858404055 27.239047806031618 27.92683168950235 23.835203646061977 235-239 21.363434816274037 27.063097961443688 26.14324382157062 25.430223400711654 240-244 22.055057124137733 30.438352477658825 23.83357121269945 23.67301918550399 245-249 20.098210720675212 29.06441714407989 24.95196454531216 25.88540758993274 250-254 20.482935556384028 26.855470085658634 25.976250300566278 26.685344057391063 255-259 20.912177291087133 27.29808012600621 27.058730099203416 24.73101248370324 260-264 20.06856220122609 28.672236058688185 26.62026732199307 24.638934418092656 265-269 19.814696753443002 28.549450597933866 26.043180068429844 25.592672580193288 270-274 18.508264745395543 30.70174780669342 24.900653193944112 25.889334253966922 275-279 19.960865719119987 29.15142143032046 23.5488660103108 27.33884684024875 280-284 17.16106602310555 30.513576771796846 26.245381206280015 26.079975998817588 285-289 19.070483619413604 28.000820628663327 27.279549801350505 25.649145950572567 290-294 18.794205302761238 31.044823531747518 24.983995535074154 25.176975630417086 295-299 18.00626942650851 31.84833370467516 22.79441707790016 27.35097979091617 300-301 13.815018828133443 34.23742199064218 26.80146742523345 25.146091755990916 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 1.0 27 1.5 28 0.75 29 0.75 30 1.5 31 4.25 32 6.25 33 8.25 34 21.5 35 68.25 36 227.5 37 957.0 38 3153.0 39 4718.25 40 7404.5 41 9710.25 42 14370.5 43 18166.75 44 21299.75 45 17734.75 46 12381.5 47 9966.5 48 8749.75 49 7085.75 50 5703.0 51 3070.5 52 1166.25 53 620.75 54 497.75 55 567.25 56 556.25 57 451.25 58 453.75 59 615.0 60 633.25 61 400.25 62 170.25 63 45.25 64 15.0 65 5.25 66 1.5 67 1.5 68 0.75 69 0.25 70 0.5 71 0.75 72 0.0 73 0.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.25 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.25 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-249 0.0 250-254 0.0 255-259 0.0 260-264 0.0 265-269 0.0 270-274 0.0 275-279 0.0 280-284 0.0 285-289 0.0 290-294 0.0 295-299 0.0 300-301 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 301 453311.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.189733018412012 #Duplication Level Percentage of deduplicated Percentage of total 1 39.99531552040423 3.675462716196652 2 6.7862730506793545 1.2472807505157537 3 4.129343555638703 1.138426944528595 4 3.7203379319171748 1.3675564933035966 5 3.4255935024287227 1.5740144858463436 6 3.471516064728491 1.914138348238989 7 3.2949086321249035 2.1195531454502294 8 2.833268009991809 2.0829581265145776 9 2.928361713608865 2.421977609646447 >10 27.974067518044244 48.42183549053128 >50 1.1939554632155343 7.2232079758221515 >100 0.17991953072820044 2.8863348551722066 >500 0.019182716213736375 1.2793814227615123 >1k 0.028774074165615157 6.8301251151799525 >5k 0.014387037082807575 9.547984296062308 >10k+ 0.004795679027602525 6.269762224229422 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC 15686 3.460317530348921 No Hit CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT 12767 2.8163887485633485 No Hit GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG 9353 2.0632634107709715 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA 8465 1.867371407267858 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA 8406 1.8543560601882594 No Hit GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT 6392 1.4100694666575486 No Hit CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT 5458 1.2040299044144087 No Hit CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG 5256 1.1594688855995112 No Hit CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA 4818 1.0628464784662184 No Hit GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT 4335 0.9562971116959439 No Hit GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG 3915 0.863645488417444 No Hit GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA 3318 0.731947823900148 No Hit GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 2788 0.6150302992868031 No Hit GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA 2375 0.5239228697296117 No Hit GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG 2221 0.48995060786082845 No Hit GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG 2149 0.47406747244165703 No Hit GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT 1567 0.3456787944700217 No Hit GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT 1282 0.2828080501024683 No Hit GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG 1193 0.2631747299315481 No Hit GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC 1035 0.22832007165058868 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT 913 0.20140698107921495 TruSeq Adapter, Index 8 (97% over 36bp) CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT 875 0.19302421516354115 No Hit GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 848 0.18706803938135186 No Hit GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA 788 0.17383209319870907 No Hit CGGGTTATTAGTAGAAACAAGGTATCGAATTTGACCGGCTTAGCCAAATA 660 0.14559540800907103 No Hit GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA 602 0.1328006600325163 No Hit CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCGTTGC 587 0.1294916734868556 No Hit GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC 533 0.11757932192247705 No Hit CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT 485 0.1069905649763628 No Hit CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG 478 0.10544637125505446 No Hit GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT 459 0.10125498829721757 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.4119820608809403E-4 0.0 0.0 2 4.4119820608809403E-4 0.0 0.0 3 6.617973091321411E-4 0.0 0.0 4 6.617973091321411E-4 0.0 0.0 5 8.823964121761881E-4 0.0 0.0 6 8.823964121761881E-4 0.0 0.0 7 8.823964121761881E-4 0.0 0.0 8 0.001102995515220235 0.0 0.0 9 0.001102995515220235 0.0 0.0 10-14 0.0020295117480052327 0.0 0.0 15-19 0.0026471892365285644 0.0 0.0 20-24 0.0028677883395726114 0.0 0.0 25-29 0.0028677883395726114 0.0 0.0 30-34 0.00445610188148975 0.0 0.0 35-39 0.005073779370013081 0.0 0.0 40-44 0.005514977576101175 0.0 0.0 45-49 0.006220894705842127 0.0 0.0 50-54 0.009574001072111643 0.0 0.0 55-59 0.012397669591075442 0.0 0.0 60-64 0.01491249936577758 0.0 0.0 65-69 0.02047159676248756 0.0 0.0 70-74 0.030177957296425632 0.0 0.0 75-79 0.042046189040195366 0.0 0.0 80-84 0.0586352415891077 0.0 0.0 85-89 0.08056279243168597 0.0 0.0 90-94 0.10981423349532662 0.0 0.0 95-99 0.145374808906027 0.0 0.0 100-104 0.1913476619804064 0.0 0.0 105-109 0.24795339182150888 0.0 0.0 110-114 0.317133270536122 0.0 0.0 115-119 0.39862257920059296 0.0 0.0 120-124 0.4958185440018001 0.0 0.0 125-129 0.6138390641303653 0.0 0.0 130-134 0.7653906479216255 0.0 0.0 135-139 0.9495908989634048 0.0 0.0 140-144 1.1746902237095505 0.0 0.0 145-149 1.4663663577543895 0.0 0.0 150-154 1.8150011802052013 0.0 0.0 155-159 2.25787593947643 0.0 0.0 160-164 2.7527238474248366 0.0 0.0 165-169 3.3608273348760562 0.0 0.0 170-174 4.049537734579571 0.0 0.0 175-179 4.859842359880965 0.0 0.0 180-184 5.785476196253787 0.0 0.0 185-189 6.964799001127261 0.0 0.0 190-194 8.158901945904688 0.0 0.0 195-199 9.40877234393165 0.0 0.0 200-204 10.717675061933198 0.0 0.0 205-209 12.152981065978985 0.0 0.0 210-214 13.545976162060924 0.0 0.0 215-219 14.900807613316243 0.0 0.0 220-224 16.379395161379275 0.0 0.0 225-229 17.891337293822566 0.0 0.0 230-234 19.377557570850918 0.0 0.0 235-239 20.803598412568853 0.0 0.0 240-244 22.26197908279305 0.0 0.0 245-249 23.50139308333572 0.0 0.0 250-254 24.829973241328798 0.0 0.0 255-259 26.05076867757456 0.0 0.0 260-264 27.249327724233474 0.0 0.0 265-269 28.3502937277057 0.0 0.0 270-274 29.43625899217094 0.0 0.0 275-279 30.31190507179398 0.0 4.41198206088094E-5 280-284 31.11325337351178 0.0 2.2059910304404702E-4 285-289 31.837789067549654 0.0 2.2059910304404702E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTTA 6700 0.0 292.13806 1 GGGTTAT 6785 0.0 289.13043 2 GTTATTA 6885 0.0 285.14523 4 GGGGGGA 5470 0.0 282.32632 1 GGTTATT 6970 0.0 281.45627 3 TTATTAG 7060 0.0 277.86826 5 TTAGTAG 7175 0.0 272.59235 8 TATTAGT 7290 0.0 268.89917 6 ATTAGTA 7325 0.0 267.61435 7 GGGAGCG 4620 0.0 264.9892 4 GGAGCGA 4625 0.0 264.7027 5 GGGGAGC 5895 0.0 261.4716 3 TAGTAGA 7485 0.0 261.3026 9 CGAAAGC 4780 0.0 256.7364 9 GAGCGAA 5005 0.0 244.6054 6 AGCGAAA 5015 0.0 244.11766 7 GCGAAAG 5035 0.0 243.73386 8 GGGGGAG 6910 0.0 224.13171 2 AAACGTT 330 0.0 223.48485 295 GGGAGCA 1850 0.0 171.41891 4 >>END_MODULE