##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_6471_flu.3510000003bb5f.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	294938
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.79073568004123	34.0	34.0	34.0	34.0	34.0
2	33.87524835728187	34.0	34.0	34.0	34.0	34.0
3	33.87928988465372	34.0	34.0	34.0	34.0	34.0
4	33.88903430551506	34.0	34.0	34.0	34.0	34.0
5	33.89386243888546	34.0	34.0	34.0	34.0	34.0
6	37.67103255599482	38.0	38.0	38.0	38.0	38.0
7	37.66869986234395	38.0	38.0	38.0	38.0	38.0
8	37.66932372227383	38.0	38.0	38.0	38.0	38.0
9	37.59421641158481	38.0	38.0	38.0	38.0	38.0
10-14	37.666751656280304	38.0	38.0	38.0	38.0	38.0
15-19	37.63263872407083	38.0	38.0	38.0	37.6	38.0
20-24	37.6203656361676	38.0	38.0	38.0	37.2	38.0
25-29	37.53104110016342	38.0	38.0	38.0	37.0	38.0
30-34	37.46369813316697	38.0	38.0	38.0	37.0	38.0
35-39	37.38551017501983	38.0	38.0	38.0	37.0	38.0
40-44	37.36410364212139	38.0	38.0	38.0	37.0	38.0
45-49	37.33837077623094	38.0	38.0	38.0	37.0	38.0
50-54	37.289736826044795	38.0	38.0	38.0	37.0	38.0
55-59	37.234457411388156	38.0	38.0	38.0	37.0	38.0
60-64	37.05846516895076	38.0	38.0	38.0	36.4	38.0
65-69	37.03460456095857	38.0	38.0	38.0	36.4	38.0
70-74	37.107045548555966	38.0	38.0	38.0	36.8	38.0
75-79	36.983776251279934	38.0	38.0	38.0	36.2	38.0
80-84	36.86595759108694	38.0	38.0	38.0	36.0	38.0
85-89	36.89839830744089	38.0	38.0	38.0	36.0	38.0
90-94	36.75460740901478	38.0	38.0	38.0	35.4	38.0
95-99	36.744822979744896	38.0	38.0	38.0	35.6	38.0
100-104	36.747716469224045	38.0	38.0	38.0	35.4	38.0
105-109	36.55878184567604	38.0	38.0	38.0	34.8	38.0
110-114	36.474249503285435	38.0	37.4	38.0	34.2	38.0
115-119	36.538603367487404	38.0	37.8	38.0	34.0	38.0
120-124	36.4262889149584	38.0	37.2	38.0	34.0	38.0
125-129	36.385659358916115	38.0	37.0	38.0	34.0	38.0
130-134	36.26669333893903	38.0	37.0	38.0	34.0	38.0
135-139	36.02430680346377	38.0	37.0	38.0	32.4	38.0
140-144	35.96050220724355	38.0	37.0	38.0	32.2	38.0
145-149	35.51819975723712	38.0	36.2	38.0	29.2	38.0
150-154	35.17565115380181	38.0	36.2	38.0	27.8	38.0
155-159	35.23050878489717	38.0	35.8	38.0	28.2	38.0
160-164	35.17480419613614	38.0	35.8	38.0	28.0	38.0
165-169	34.89705293993992	38.0	35.2	38.0	26.8	38.0
170-174	34.80575036109284	38.0	35.0	38.0	26.4	38.0
175-179	34.497542534363156	38.0	35.0	38.0	24.0	38.0
180-184	34.13133811173874	37.6	34.6	38.0	23.0	38.0
185-189	33.373242511985566	37.0	33.4	37.8	22.0	38.0
190-194	32.45852687683514	36.8	30.4	37.0	20.2	38.0
195-199	32.212803368843616	36.8	29.8	37.2	20.0	37.8
200-204	32.132597359445036	37.0	29.8	37.0	20.0	37.4
205-209	30.94062955604229	35.4	27.4	37.0	17.6	37.2
210-214	30.230196854932224	34.2	25.8	37.0	16.0	37.0
215-219	29.482769259980063	33.2	24.4	37.0	14.0	37.0
220-224	28.0614176538798	31.6	22.0	36.2	9.8	37.0
225-229	26.926109216174247	29.8	20.0	36.0	9.2	37.0
230-234	26.635816341061513	29.0	20.0	35.8	9.0	37.0
235-239	25.783901701374525	28.4	18.8	34.6	9.0	37.0
240-244	24.734034271609623	26.6	17.0	33.6	8.8	37.0
245-249	23.880206009398584	25.0	16.2	33.0	8.2	36.2
250-254	23.156581383205964	24.0	15.0	32.0	8.0	36.0
255-259	22.49084349931172	23.0	14.0	31.2	8.0	36.0
260-264	21.390113854437203	21.4	12.4	30.0	8.0	36.0
265-269	20.629383802697518	20.6	11.6	28.8	7.8	35.0
270-274	19.477307773159104	19.0	9.2	27.2	7.0	33.8
275-279	18.552441530084288	18.0	8.6	26.0	7.0	32.8
280-284	18.332543110755484	18.0	8.4	25.6	7.0	31.6
285-289	17.09415199126596	15.8	8.0	23.6	7.0	30.4
290-294	16.272202971471973	14.6	8.0	22.0	7.0	29.2
295-299	15.532502424238317	13.0	8.0	21.0	7.0	27.0
300-301	13.72261288813242	12.0	8.0	18.0	7.0	24.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.054231775746117705
1101	2	0.0060688971611284614
1101	3	-0.061483226265245605
1101	4	-0.07354918972735192
1101	5	0.028949478142024532
1101	6	0.1657659535718068
1101	7	0.07786457422881199
1101	8	0.03647807296123773
1101	9	0.17394494927801674
1101	10-14	0.09298170872178702
1101	15-19	0.12439197926979517
1101	20-24	0.02783198677274612
1101	25-29	0.0641718911249427
1101	30-34	0.16275927600279516
1101	35-39	0.024769511839132008
1101	40-44	0.10928209193615146
1101	45-49	0.10156222156083317
1101	50-54	0.045481283066564515
1101	55-59	0.11036667724281557
1101	60-64	0.3342885483872351
1101	65-69	0.22770274141549152
1101	70-74	0.20001196213878814
1101	75-79	0.09739791844030066
1101	80-84	0.1723448557025975
1101	85-89	0.16485000855436027
1101	90-94	0.17302361912065578
1101	95-99	0.11342162186408444
1101	100-104	0.1354553013877151
1101	105-109	0.16681349290824699
1101	110-114	0.32881502740246304
1101	115-119	0.18057540915894066
1101	120-124	0.29210035490304875
1101	125-129	0.42702674764377235
1101	130-134	0.3184620952137607
1101	135-139	0.2540625923552753
1101	140-144	0.24198205940868434
1101	145-149	0.5925344931755561
1101	150-154	0.6806799711147633
1101	155-159	0.5889250182505066
1101	160-164	0.47231805798507764
1101	165-169	0.557130706999466
1101	170-174	0.4476906586125864
1101	175-179	0.6482438503004957
1101	180-184	0.27735644780060653
1101	185-189	0.32980738160718914
1101	190-194	0.5765692887060752
1101	195-199	0.33943518104609893
1101	200-204	0.17343701393902222
1101	205-209	-0.05594282728242206
1101	210-214	-0.23505151116987832
1101	215-219	0.0922845616634369
1101	220-224	0.5805078786309927
1101	225-229	0.8832968637263257
1101	230-234	0.5077619781075491
1101	235-239	0.4880584315379686
1101	240-244	0.90103729026848
1101	245-249	1.0180471596573533
1101	250-254	1.0588346616995494
1101	255-259	-0.14359531187741226
1101	260-264	0.4669317647383515
1101	265-269	0.604996089423981
1101	270-274	0.373798488015435
1101	275-279	1.0362888213486876
1101	280-284	0.4546240966848245
1101	285-289	0.6476651149409101
1101	290-294	0.5036197293586859
1101	295-299	0.5159784494235495
1101	300-301	0.6111454798383456
1102	1	-0.17173447297696498
1102	2	-0.053090365995359434
1102	3	-0.00451171701474351
1102	4	0.027353883117861244
1102	5	-0.0208826941610738
1102	6	0.02592618906336952
1102	7	-0.01077109315267677
1102	8	4.4173693289195626E-4
1102	9	0.1565827744441819
1102	10-14	0.056613713197194215
1102	15-19	0.053524070322971795
1102	20-24	0.006212849087425809
1102	25-29	-0.010746525966844445
1102	30-34	0.18199619276824563
1102	35-39	0.07636986880596908
1102	40-44	0.006102689456035648
1102	45-49	0.07800773749780632
1102	50-54	0.04694444755103433
1102	55-59	-0.03644393053319561
1102	60-64	0.054812144162248444
1102	65-69	0.10568867585613617
1102	70-74	0.06209222640080725
1102	75-79	-0.02417127052569157
1102	80-84	0.056980629641365965
1102	85-89	0.021726282246703477
1102	90-94	0.27736782573519747
1102	95-99	0.06658903665157112
1102	100-104	0.07916908856621063
1102	105-109	0.1697331620479261
1102	110-114	0.12827491525094104
1102	115-119	0.07032659793846108
1102	120-124	0.02068367602670662
1102	125-129	0.021361230907629647
1102	130-134	0.23650024271567105
1102	135-139	0.14333427342936744
1102	140-144	0.21604135852825124
1102	145-149	0.47797277653796755
1102	150-154	0.9266108154479227
1102	155-159	0.5745684243535791
1102	160-164	0.6108498320303966
1102	165-169	0.6739740811353485
1102	170-174	0.5167204546886168
1102	175-179	0.4086984502445503
1102	180-184	0.6368200651534508
1102	185-189	0.8529343046328819
1102	190-194	0.7932455011279771
1102	195-199	0.8327713559065728
1102	200-204	0.3140226793225622
1102	205-209	0.3313189173264455
1102	210-214	-0.21305342920069847
1102	215-219	-0.004095155160616315
1102	220-224	1.3174326358802162
1102	225-229	1.1269467165440972
1102	230-234	0.8597346195290179
1102	235-239	0.8959330136336803
1102	240-244	1.2272483802161318
1102	245-249	1.1019670019525982
1102	250-254	0.6137629353386131
1102	255-259	0.8566684173605665
1102	260-264	1.106690945312959
1102	265-269	1.1509582349545724
1102	270-274	1.185186796019213
1102	275-279	1.3576255822741352
1102	280-284	0.8009432090627797
1102	285-289	1.336026397357628
1102	290-294	1.1698610549311006
1102	295-299	0.9994507511937272
1102	300-301	0.47404623506298194
1103	1	-0.04126808639488644
1103	2	-0.006948280780825655
1103	3	-0.06962321725323051
1103	4	0.0018065094009500626
1103	5	-0.001093280313220646
1103	6	0.04646046240066681
1103	7	0.06218593368186731
1103	8	0.04978214425342742
1103	9	0.05226650971850688
1103	10-14	0.06106522872489961
1103	15-19	0.06357869335220556
1103	20-24	0.032403154153129776
1103	25-29	0.14272491128528486
1103	30-34	0.14494433355975644
1103	35-39	-0.2663889862012425
1103	40-44	-0.12030649975788066
1103	45-49	-0.05548676426307253
1103	50-54	0.1173300756831992
1103	55-59	-0.24443222186473434
1103	60-64	0.26693603912398345
1103	65-69	0.29187316579072586
1103	70-74	0.23996793940775518
1103	75-79	0.4075221740574122
1103	80-84	0.27657304775582503
1103	85-89	0.20170851016064262
1103	90-94	0.2835099762882862
1103	95-99	0.39965981790464156
1103	100-104	0.3555465885825484
1103	105-109	0.22402035111809937
1103	110-114	0.3616554012390907
1103	115-119	0.22582157013690818
1103	120-124	0.4756239239211624
1103	125-129	0.528331220910836
1103	130-134	0.5705256202091462
1103	135-139	0.5292273534233587
1103	140-144	0.5873358459146445
1103	145-149	0.7610138287500021
1103	150-154	0.9783514344653597
1103	155-159	0.05854447124052342
1103	160-164	-0.46357894568131996
1103	165-169	0.5144980309814784
1103	170-174	0.9096988472295706
1103	175-179	0.6668095780532894
1103	180-184	0.8554588671057743
1103	185-189	0.6634329031209489
1103	190-194	0.5173194905039367
1103	195-199	0.7946195221801204
1103	200-204	0.563436592513753
1103	205-209	0.9899396858384897
1103	210-214	0.8082570661308566
1103	215-219	0.5342932041230064
1103	220-224	1.0635283307230452
1103	225-229	2.0007694791887403
1103	230-234	0.6924943892759643
1103	235-239	1.3968882632596227
1103	240-244	1.3426672983403947
1103	245-249	1.082277556088858
1103	250-254	1.0916069591479811
1103	255-259	0.6442874151978621
1103	260-264	1.7153110604394932
1103	265-269	0.8222635032019987
1103	270-274	1.1221220259129119
1103	275-279	1.9733885078731248
1103	280-284	0.7336961182617934
1103	285-289	0.5476181098157298
1103	290-294	1.0619479029340262
1103	295-299	1.2927734779021698
1103	300-301	-0.7733418563325856
2116	1	0.033411186323029085
2116	2	0.04901653894278013
2116	3	0.016345848809514507
2116	4	0.022245233440372658
2116	5	0.010416872297632551
2116	6	-0.06643264566223195
2116	7	-0.04148907982921202
2116	8	0.10002405690751459
2116	9	0.11833729490521705
2116	10-14	0.14129305125365477
2116	15-19	0.19723639981436492
2116	20-24	-0.08302263785345332
2116	25-29	-0.1429237491877302
2116	30-34	0.033357618167421776
2116	35-39	-0.03420430905169525
2116	40-44	-0.0858014556216844
2116	45-49	0.14235821862290265
2116	50-54	0.18740786089563954
2116	55-59	0.21972610224675293
2116	60-64	0.32989541581287085
2116	65-69	0.27055332637955587
2116	70-74	-0.5537201348131049
2116	75-79	0.32042268104456895
2116	80-84	-0.025536289043209592
2116	85-89	0.2379259227246564
2116	90-94	0.3812459054644961
2116	95-99	0.3615781981192683
2116	100-104	0.3656248885027722
2116	105-109	0.38434667584723314
2116	110-114	0.4816427908986114
2116	115-119	0.4526571467382965
2116	120-124	0.035383105021097094
2116	125-129	0.14765656769519353
2116	130-134	0.4211082050715831
2116	135-139	0.642432824766999
2116	140-144	0.4869134638470669
2116	145-149	0.806618224354402
2116	150-154	1.148717456031612
2116	155-159	-0.0885197642897495
2116	160-164	-0.32939945421260575
2116	165-169	0.8515145273656444
2116	170-174	1.0850745839050688
2116	175-179	0.9953472289270096
2116	180-184	0.9172723756113186
2116	185-189	1.300656891076784
2116	190-194	0.9966279162702065
2116	195-199	1.009262314984504
2116	200-204	1.2582174556786967
2116	205-209	1.0553249446078254
2116	210-214	1.1874197652584293
2116	215-219	1.559893160372848
2116	220-224	1.7173625819519138
2116	225-229	1.4035804270231083
2116	230-234	1.9104113669119158
2116	235-239	1.6759676440661693
2116	240-244	2.2951253496567574
2116	245-249	1.5632179271931612
2116	250-254	1.2154614126570031
2116	255-259	1.508696015964773
2116	260-264	0.7484444083562103
2116	265-269	0.9061660175494737
2116	270-274	1.2041661235015013
2116	275-279	2.471656194519035
2116	280-284	0.3130955829657083
2116	285-289	-1.2314355356020812
2116	290-294	0.3016576077491173
2116	295-299	0.7886307237008694
2116	300-301	1.329054430050821
1117	1	-0.07067501337424176
1117	2	0.03477388535960557
1117	3	0.057031688362727095
1117	4	0.04631681859617487
1117	5	0.05364720791013866
1117	6	-0.4344418058480386
1117	7	-0.21759430452779327
1117	8	-0.07322350298600355
1117	9	-0.02306818032835878
1117	10-14	0.10753933237480595
1117	15-19	0.07120359538824061
1117	20-24	0.01912778753409583
1117	25-29	0.12814206844365827
1117	30-34	-0.0012227357132630345
1117	35-39	-0.12615587228567904
1117	40-44	-0.057252862271298
1117	45-49	0.07861598259805902
1117	50-54	0.08936059851242817
1117	55-59	0.1587364890101597
1117	60-64	0.11344948961630763
1117	65-69	0.26384499087144064
1117	70-74	-0.14621648117774555
1117	75-79	0.14057900444350224
1117	80-84	0.034374849325189416
1117	85-89	0.07277116516945625
1117	90-94	0.25157969115268486
1117	95-99	0.2190949004523759
1117	100-104	0.22492926242624378
1117	105-109	0.14579560324427376
1117	110-114	0.32090110291906626
1117	115-119	0.2009608160321008
1117	120-124	0.3782330710944706
1117	125-129	0.4454187160327905
1117	130-134	0.21306955482888412
1117	135-139	0.17399631973020746
1117	140-144	0.29775888897363956
1117	145-149	0.8401403288483777
1117	150-154	0.743289850206672
1117	155-159	0.014571472204849556
1117	160-164	-0.6101598018299157
1117	165-169	0.2844938060336446
1117	170-174	0.5187128218089256
1117	175-179	0.6190273531506136
1117	180-184	0.6991757891506438
1117	185-189	0.7844928167514098
1117	190-194	1.06269543023231
1117	195-199	0.7427126516409004
1117	200-204	0.8256687840361252
1117	205-209	1.555595052651043
1117	210-214	0.8768986002707031
1117	215-219	0.33670497205404004
1117	220-224	1.053222360646
1117	225-229	0.8239637977627083
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2108	170-174	-0.5096148012829715
2108	175-179	-0.6738958079103767
2108	180-184	-0.2599182931090027
2108	185-189	-0.9887497961263989
2108	190-194	-0.5625624957436557
2108	195-199	-1.3154796950270438
2108	200-204	-0.8737424981141366
2108	205-209	-0.4556961053215822
2108	210-214	-0.08383617107836727
2108	215-219	-0.8823302384206322
2108	220-224	-0.53286749120943
2108	225-229	-0.6990219346762885
2108	230-234	-0.5620260608894014
2108	235-239	-0.4991000622616468
2108	240-244	-0.155481440229007
2108	245-249	-0.2615260081168138
2108	250-254	-0.12357176665246072
2108	255-259	-0.7857045744600732
2108	260-264	0.24798009055361803
2108	265-269	-0.9588628619268427
2108	270-274	-0.9863399091002165
2108	275-279	-0.365267499486869
2108	280-284	-0.8393155324238428
2108	285-289	0.1459348276375536
2108	290-294	-0.5562861735369822
2108	295-299	-0.14475330915755968
2108	300-301	-0.5040976043478445
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	8.0
13	55.0
14	181.0
15	328.0
16	421.0
17	501.0
18	1055.0
19	1031.0
20	914.0
21	1086.0
22	1515.0
23	2277.0
24	3273.0
25	4754.0
26	6362.0
27	8597.0
28	11933.0
29	16528.0
30	23568.0
31	33952.0
32	46142.0
33	55541.0
34	50277.0
35	22510.0
36	2128.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.2795367161912	18.968054302938246	14.209427066027436	33.54298191484312
2	50.79304803043352	21.207168964324705	14.723094345252221	13.276688659989558
3	50.63572683072375	19.560043127708195	16.876428266279692	12.927801775288367
4	50.94901301290441	19.589201798344057	16.25968847690023	13.202096711851304
5	31.684964297581185	19.46137832357987	35.876353674331554	12.977303704507387
6	30.08293268415735	20.16661128779608	36.15234388244309	13.598112145603483
7	15.525635896357878	53.14879737436342	17.788484359424693	13.537082369854003
8	29.368884307888436	18.81446270063539	37.369209800025764	14.447443191450407
9	14.674609578962357	17.2975337189511	38.82714333181889	29.20071337026765
10-14	27.989475754226312	35.8708609945141	21.807701957699585	14.331961293560003
15-19	22.017712197139737	37.57637198326428	19.071398056540698	21.334517763055285
20-24	26.992045785893986	30.368212980355192	25.188480290772976	17.451260942977846
25-29	19.005757142179032	26.03638730851908	37.80116499060819	17.15669055869369
30-34	18.512907797571014	30.36563616760133	30.10653086411381	21.014925170713845
35-39	20.737239691053713	31.195573306932307	28.059727807200158	20.007459194813826
40-44	20.072150757108272	32.031070936942676	27.04615885372519	20.850619452223857
45-49	21.090737714367087	29.991320209671184	26.214323010259783	22.703619065701943
50-54	22.998121639124154	31.33539930426056	25.110362177813645	20.556116878801646
55-59	22.09705090561406	27.142585899409365	28.347449294427978	22.41291390054859
60-64	20.416290881473394	27.25630471488923	29.178200164102286	23.14920423953509
65-69	18.188432823169613	28.597061077243353	29.248655649661963	23.965850449925068
70-74	21.050864927544094	25.38147000386522	31.291390054858987	22.2762750137317
75-79	21.803294251673233	24.901301290440703	29.336674148465104	23.958730309420964
80-84	24.0959116831334	27.808149509388414	24.521628274416997	23.574310533061187
85-89	22.807505306199946	27.606073140795694	25.423173683960698	24.163247869043662
90-94	22.348629203425805	30.602431697509306	27.72528463609301	19.323654462971877
95-99	23.974733672839715	27.495541435827192	28.312391078803	20.217333812530093
100-104	21.761251517268036	27.75512141534831	27.544433067288722	22.939194000094933
105-109	25.383775573171313	28.73878577870603	24.30449789447274	21.57294075364992
110-114	20.500037295974067	27.54809485383369	31.11948951983129	20.832378330360957
115-119	24.849765035363365	26.296916640107415	27.682835036516153	21.170483288013074
120-124	20.774942530294503	27.939499148973685	26.45830649153381	24.827251829198
125-129	19.972536600912736	25.670818951779694	30.049434118357077	24.30721032895049
130-134	23.67399250011867	28.08454658267161	27.28186940984207	20.95959150736765
135-139	22.540059266693337	26.229105778163547	25.45918125165289	25.771653703490227
140-144	21.62061178959646	27.526666621459427	26.37422102272342	24.4785005662207
145-149	23.28957272375889	24.343421329228516	29.57713146491805	22.78987448209454
150-154	21.759217191409718	28.244241162549415	25.239880924126428	24.756660721914436
155-159	22.258712000488238	26.038625066963228	31.12457533447708	20.57808759807146
160-164	22.360089239094318	30.581206897720875	25.363364503726203	21.6953393594586
165-169	21.35709878008259	28.855962948145038	25.73476459459276	24.05217367717961
170-174	21.02679207155402	31.0502546297866	26.515335426428603	21.407617872230773
175-179	20.4781343875662	29.06115861638717	26.805023428652802	23.655683567393826
180-184	21.55368246885786	28.386372729183762	27.359512846767796	22.700431955190584
185-189	21.331195030820034	26.882599054716582	29.284798839078043	22.501407075385334
190-194	20.498545457011303	26.895211875038143	28.25570119821793	24.350541469732622
195-199	18.856505435040585	32.19713973784321	27.290752632756714	21.655602194359492
200-204	21.446880361296273	27.527615973526636	25.505428259498608	25.520075405678483
205-209	22.05283822362666	25.186852830086327	29.20396829164095	23.55634065464606
210-214	23.0147353003004	27.029680814272833	28.97110579172572	20.984478093701046
215-219	21.323464592558437	28.200299724009792	29.148159952261153	21.328075731170618
220-224	20.57951162617228	27.041412093389116	28.021685913649648	24.35739036678895
225-229	23.572547450650646	27.58851012755223	27.587832018932794	21.25111040286433
230-234	22.301297221788985	26.964039899911167	28.149441577551894	22.585221300747953
235-239	21.849948124690613	27.243556272843783	26.55208891360218	24.354406688863424
240-244	22.65859265337122	30.90785181970448	24.936562938651512	21.49699258827279
245-249	22.809607442920207	27.94892485878388	25.533637578067253	23.70783012022866
250-254	20.030786131322515	28.068068543219255	24.941445320711473	26.95970000474676
255-259	22.79543497277394	28.874610935179597	24.897164827862127	23.432789264184336
260-264	20.661698390848247	27.641334789006507	28.094107914205697	23.602858905939552
265-269	21.2298856030759	28.451132102340154	25.367026290271177	24.95195600431277
270-274	19.079603170835906	31.002651404702004	25.922600682177272	23.99514474228482
275-279	20.546284303819785	28.345550590293556	25.95243746143257	25.15572764445409
280-284	17.573523927062638	29.89190948606148	29.007520224589573	23.52704636228631
285-289	19.860784300429245	28.901531847371313	26.778170327323032	24.459513524876414
290-294	21.36760946368389	29.33443639002095	26.180960066183403	23.116994080111752
295-299	19.80619655656443	29.638229051529475	25.313523520197467	25.24205087170863
300-301	15.734154296835268	32.692803233221895	26.973296082566506	24.59974638737633
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.5
30	1.0
31	1.5
32	6.5
33	25.75
34	97.25
35	215.75
36	403.5
37	939.75
38	2459.25
39	5205.5
40	5141.5
41	6166.25
42	7215.75
43	10909.25
44	12819.75
45	9971.75
46	8949.75
47	8699.75
48	6039.0
49	4433.75
50	3669.5
51	1336.25
52	275.5
53	116.5
54	123.5
55	173.75
56	170.75
57	196.25
58	437.25
59	632.0
60	511.75
61	450.25
62	241.75
63	55.0
64	13.25
65	5.5
66	2.25
67	0.75
68	0.5
69	0.25
70	1.0
71	0.25
72	0.5
73	0.25
74	0.0
75	0.0
76	0.0
77	0.0
78	0.25
79	0.0
80	0.0
81	0.25
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	294938.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.16145718151902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	39.33295583733751	4.783460582383587
2	12.946564623206964	3.1489818262580087
3	9.006441780561332	3.285943682164217
4	6.890163361146771	3.3517770676103056
5	4.885883361914022	2.970973064990679
6	4.043516822705017	2.950503402124735
7	2.8570113819972875	2.432179511249075
8	2.389732629720592	2.3250104841300665
9	2.064668482952183	2.2598439614509327
>10	14.750270305587554	33.332991053887035
>50	0.646618045219019	5.063426630877046
>100	0.10283535256862271	2.292938546717579
>500	0.01944553685271602	1.4837811317653673
>1k	0.04444694137763629	12.173289053028501
>5k	0.016667603016613606	13.17294942845967
>10k+	0.002777933836102268	4.971950572903185
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	14717	4.989862276139392	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	9524	3.2291532457669065	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	6931	2.349985420664682	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA	5929	2.0102530023259124	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	5706	1.9346438912585018	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	5493	1.8624253232882841	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	5409	1.8339447612718607	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	4376	1.4837016593317918	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	3947	1.338247360462199	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	3562	1.2077114512202565	No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT	3097	1.0500511972007676	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	3017	1.0229268524232211	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	2860	0.9696953257972861	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	2559	0.8676399785717677	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	1836	0.6225037126446915	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	1736	0.5885982816727584	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	1571	0.5326543205690687	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	1535	0.5204483654191728	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT	1446	0.4902725318541524	TruSeq Adapter, Index 1 (97% over 36bp)
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	1238	0.4197492354325316	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	1117	0.37872366395649254	No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	1071	0.36312716570940334	No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	1065	0.36109283985108737	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	724	0.24547532023679552	No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	700	0.23733801680353156	No Hit
GGGGGGAGCAAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT	664	0.2251320616536357	No Hit
GGGGGGAGCGAAAGCAGGTGAAGCATCGACAACCATCTCAATTTCATTAT	645	0.2186900297689684	No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG	577	0.19563433670805389	No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	576	0.19529528239833457	No Hit
GGGGGGAGCGAAAGCAGGCCTTTACTACAAACATGGGGTTGACAAGGTTG	506	0.1715614807179814	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	470	0.1593555255680855	No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	454	0.1539306566125762	No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	440	0.14918389627650558	No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	393	0.13324834371969702	No Hit
GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT	316	0.10714116187130855	No Hit
GGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTGGA	313	0.10612399894215055	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	3.3905430971933084E-4	0.0	0.0
9	6.781086194386617E-4	0.0	0.0
10-14	0.0018308932724843865	0.0	0.0
15-19	0.0021021367202598514	0.0	0.0
20-24	0.0026446236158107804	0.0	0.0
25-29	0.0027124344777546467	0.0	0.0
30-34	0.004272084302463569	0.0	0.0
35-39	0.004543327750239034	0.0	0.0
40-44	0.0057639232652286244	0.0	0.0
45-49	0.007323573089937546	0.0	0.0
50-54	0.011460035668513382	0.0	0.0
55-59	0.01288406376933457	0.0	0.0
60-64	0.016545850314303345	0.0	0.0
65-69	0.021156988926486243	0.0	0.0
70-74	0.030514887874739778	0.0	0.0
75-79	0.045094223192671	0.0	0.0
80-84	0.062453803850300735	0.0	0.0
85-89	0.08205114295207808	0.0	0.0
90-94	0.10815832480046654	0.0	0.0
95-99	0.14016505163797136	0.0	0.0
100-104	0.17664729536377136	0.0	0.0
105-109	0.21536729753371894	0.0	0.0
110-114	0.2610718184838848	0.0	0.0
115-119	0.31504926459120225	0.0	0.0
120-124	0.3754009317212431	0.0	0.0
125-129	0.45534993795306133	0.0	0.0
130-134	0.5489289274355966	0.0	0.0
135-139	0.6490177596647431	0.0	0.0
140-144	0.7589391668757501	0.0	0.0
145-149	0.8957136754165281	0.0	0.0
150-154	1.0590022309773581	0.0	0.0
155-159	1.2484657792485199	0.0	0.0
160-164	1.4778021143426756	0.0	0.0
165-169	1.7620652476113623	0.0	0.0
170-174	2.0527704127647164	0.0	0.0
175-179	2.39853799781649	0.0	0.0
180-184	2.7912306993334193	0.0	0.0
185-189	3.2607531074327483	0.0	0.0
190-194	3.792729319382379	0.0	0.0
195-199	4.366544833151375	0.0	0.0
200-204	5.03929639449647	0.0	0.0
205-209	5.822172795638406	0.0	0.0
210-214	6.656721073581567	0.0	0.0
215-219	7.424679085095851	0.0	0.0
220-224	8.313747296041878	0.0	0.0
225-229	9.292664899063533	0.0	0.0
230-234	10.339596796614881	0.0	0.0
235-239	11.338043927876367	0.0	0.0
240-244	12.401182621432302	0.0	0.0
245-249	13.41705714421336	0.0	0.0
250-254	14.498776013941916	0.0	0.0
255-259	15.553777404064581	0.0	0.0
260-264	16.590469861462406	0.0	0.0
265-269	17.603292895455997	0.0	0.0
270-274	18.576243142626588	0.0	0.0
275-279	19.4212342933091	0.0	2.034325858315985E-4
280-284	20.226895144064176	0.0	3.3905430971933084E-4
285-289	20.986919284731027	0.0	3.3905430971933084E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	5510	0.0	290.1815	1
GGGGGGA	4655	0.0	289.29645	1
GGGTTAT	5565	0.0	288.1087	2
ATTAGTA	5670	0.0	282.25308	7
TTATTAG	5710	0.0	280.27582	5
TATTAGT	5760	0.0	278.09894	6
GGTTATT	5895	0.0	271.98047	3
GGAGCGA	3960	0.0	269.67172	5
CGAAAGC	3970	0.0	269.36398	9
TAGTAGA	5955	0.0	268.24936	9
GCGAAAG	3995	0.0	267.67834	8
TTAGTAG	5980	0.0	267.12793	8
GAGCGAA	4035	0.0	265.02478	6
GTTATTA	6090	0.0	263.2718	4
GGGGGAG	5155	0.0	261.23666	2
AGCGAAA	4295	0.0	248.98137	7
GGGAGCG	4290	0.0	248.92773	4
GGGGAGC	5575	0.0	240.49774	3
GGCTTAT	15	0.0028486268	196.66667	295
GGACCGC	15	0.0028486268	196.66667	295
>>END_MODULE