FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_6471_flu.3510000003bb5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_6471_flu.3510000003bb5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294938
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA147174.989862276139392No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC95243.2291532457669065No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG69312.349985420664682No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA59292.0102530023259124No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG57061.9346438912585018No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC54931.8624253232882841No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG54091.8339447612718607No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG43761.4837016593317918No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA39471.338247360462199No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA35621.2077114512202565No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT30971.0500511972007676No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT30171.0229268524232211No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA28600.9696953257972861No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC25590.8676399785717677No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT18360.6225037126446915No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG17360.5885982816727584No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT15710.5326543205690687No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA15350.5204483654191728No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT14460.4902725318541524TruSeq Adapter, Index 1 (97% over 36bp)
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG12380.4197492354325316No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG11170.37872366395649254No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA10710.36312716570940334No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA10650.36109283985108737No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC7240.24547532023679552No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA7000.23733801680353156No Hit
GGGGGGAGCAAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT6640.2251320616536357No Hit
GGGGGGAGCGAAAGCAGGTGAAGCATCGACAACCATCTCAATTTCATTAT6450.2186900297689684No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG5770.19563433670805389No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC5760.19529528239833457No Hit
GGGGGGAGCGAAAGCAGGCCTTTACTACAAACATGGGGTTGACAAGGTTG5060.1715614807179814No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT4700.1593555255680855No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA4540.1539306566125762No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA4400.14918389627650558No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA3930.13324834371969702No Hit
GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT3160.10714116187130855No Hit
GGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTGGA3130.10612399894215055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA55100.0290.18151
GGGGGGA46550.0289.296451
GGGTTAT55650.0288.10872
ATTAGTA56700.0282.253087
TTATTAG57100.0280.275825
TATTAGT57600.0278.098946
GGTTATT58950.0271.980473
GGAGCGA39600.0269.671725
CGAAAGC39700.0269.363989
TAGTAGA59550.0268.249369
GCGAAAG39950.0267.678348
TTAGTAG59800.0267.127938
GAGCGAA40350.0265.024786
GTTATTA60900.0263.27184
GGGGGAG51550.0261.236662
AGCGAAA42950.0248.981377
GGGAGCG42900.0248.927734
GGGGAGC55750.0240.497743
GGCTTAT150.0028486268196.66667295
GGACCGC150.0028486268196.66667295
Produced by FastQC (version 0.11.2)