FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_6271_flu.3510000003bb78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_6271_flu.3510000003bb78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362286
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA4384912.103421054084343No Hit
GGGGGGAGCGAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAAT219426.056540964873056No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG150074.142307458748061No Hit
CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG149394.123537757462337No Hit
GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG136213.7597367825419696No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT88062.4306763165013274No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC57731.5934924341542318No Hit
GGGGGGAGCAAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAAT52981.4623805501730676No Hit
GGGGGGAGCGAAAGCAGGCAGTATTCCGTATGGCATTTAAAATGCACAAT50461.392822245408324No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTG42211.16510160480946No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC35940.9920339179543233No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAATATCGAAAGAATAAAAGA34020.9390371143240422No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGTATGCT31720.8755513599752681No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTGACCGGCTTGGCCAAATA25590.7063480233848396No Hit
GGGGGGAGCGAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC24970.6892344722125614No Hit
GGGGGGAGCAAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG23710.6544553198301893No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT21650.5975941659352004No Hit
GGGGGGAGCAAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT20750.5727519142335061No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCGTTGC20730.5721998641956907No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT18150.5009854093175006TruSeq Adapter, Index 1 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGGACCAAAACCAAAACAAGAAACTAGCTG15770.4352914548174647No Hit
GGGGGGAGCGAAAGCAGGGGAGCGAACGGGATTAGATACCCCGGTAGTCC13400.3698735253363365No Hit
GGGGGGAGCAAAAGCAGGCAGTATTCCGTATGGCATTTAAAATGCACAAT13080.3610407247312896No Hit
GGGGGGAGCGAAAGCAGGGATACCGTCTTTGTTACGTTTAAAATCTACAA10710.29562279525016144No Hit
GGGGGGAGCGAAAGCAGGTCAAGTACAATACCGTACAGATAAAGCCGGTA10570.29175844498545345No Hit
GGGGGGAGCGAAAGCAGGGGGAGCGAACAGGATTAGATACCCTGGTAGTC10540.29093036992873034No Hit
GGGGGGAGCGAAAGCAGGGTTTGTTGGGGTTTTCGCAGTCTTTGGCCTTG10470.28899819479637634No Hit
GGGGGAGCGAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAATA9760.26940041845392865No Hit
GGGGGGAGCGAAAGCAGGTGTTGAAGCCGGTCGCGGTTTGGTTGAATTTG9570.2641559430946821No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA9350.2580833926787124No Hit
GGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGT9340.25780736765980466No Hit
GGGGAGCGAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAATAC9280.2561512175463584No Hit
GGGGGGAGCGAAAGCAGGTGTTGAAGCCGGCCGTGGTTTGGTTGAATTTG9270.25587519252745067No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC8910.245938291846773No Hit
GGGGGGAGCGAAAGCAGGAAAGGAACATCCAAGACATTCTCTCGCTTTGC8890.24538624180895757No Hit
GGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCT8830.2437300916955113No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAATATCGAAAGAATAAAAGA8000.22082001512617103No Hit
CGGGTTATTAGTAGAAACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAA7410.2045345390106159No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG6980.1926654631975842No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAACATTCT6850.18907713795178396No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGGCGTGCT6640.18328061255472194No Hit
GGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGGC6410.17693203711984454No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTAATATCCGTGGGAAATCTCATC6200.17113551172278255No Hit
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCTGTAAACG6150.16975538662824396No Hit
CGGGTTATTAGTAGAAACAAGGGCACCACGGTGACGTCCACCAATTTCAC6110.16865128655261313No Hit
CGGGTTATTAGTAGAAACAAGGAATGTGGAACAAATTTACTTCTAACAGA6030.16644308640135141No Hit
GGGGGGAGCGAAAGCAGGGGTAGCAAACAGGATTAGATACCCTGGTAGTC5910.16313078617445884No Hit
GGGGGGAGCGAAAGCAGCATAACGCCTGCCGTATCTTTATCCAAGCGATG5670.1565061857206737No Hit
GGGGGGAGCGAAAGCAGGACTTAGTGATCCGGTGGTTCCGCATGGAAGGG5670.1565061857206737No Hit
GGGGGGAGCGAAAGCAGGTCTACATATGGGCCTTTTTTTAATGAACGAGC5600.15457401058831974No Hit
GGGGAGCAAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAATAC5510.1520897854181503No Hit
GGGGGGAGCGAAAGCAGGGACGAGGCGAAAGTGACGGTACCTGCAGAAGA5510.1520897854181503No Hit
CGGGTTATTAGTAGAAACAAGGCCGTCTGAAAAAAACCTAAAAACCGAGT5440.1501576102857963No Hit
GGGGGGAGCGAAAGCAGAAGAGACTAAACCTGTGGTGTGTGATAGCGGTT5400.14905351021016544No Hit
CGGGTTATTAGTAGAAACAAGGCCGCGTGGAGGTTAGACTACGGTCTAGC5140.1418768597185649No Hit
GGGGGGAGCAAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC5110.14104878466184176No Hit
GGGGGGAGCGAAAGCAGGAGGTGAAAGTAAGTTGTTCAGCATTCCATTTC5010.13828853447276462No Hit
GGGGGGAGCGAAAGCAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGA4660.1286276588109946No Hit
GGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAATAC4620.12752355873536378No Hit
GGGGGGAGCAAAAGCAGGTCAAGTACAATACCGTACAGATAAAGCCGGTA4480.12365920847065578No Hit
CGGGTTATTAGTAGAAACAAGGTCGATACAGCCCCAGGTTGTGTAGCCCC4360.12034690824376322No Hit
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG4330.11951883318704007No Hit
GGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGTG4310.11896678314922465No Hit
GGGGGGAGCGAAAGCAGGTGACTTGATTGCCGTTTCAGGTATGGAAGATA4270.11786268307359378No Hit
GGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAATA4060.1120661576765318No Hit
GGGGGGAGCGAAAGCAGGTTTCATGATGTTATTGGTTATCCAAATCAGAT3980.10985795752527008No Hit
GGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATAA3760.10378540710930038No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA133300.0285.041261
CGGGTTA91700.0284.705571
TATTAGT92750.0281.64156
GGGTTAT92950.0281.03552
TTATTAG93000.0280.72585
GTTATTA93500.0279.54014
GGTTATT93750.0278.794683
TAGTAGA94000.0277.896279
ATTAGTA94100.0277.75777
TTAGTAG95650.0273.56518
GGGGGAG143150.0266.149142
CGAAAGC120100.0254.594129
AGCGAAA120700.0253.328497
GCGAAAG120700.0253.206288
GGGGAGC150550.0253.06713
GGGAGCG122050.0251.130694
GGAGCGA123050.0248.850075
GAGCGAA123250.0248.087226
GGGAGCA31250.0239.7764
CCAGTCC27600.0236.21376295
Produced by FastQC (version 0.11.2)