##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_6205_flu.3510000003ba50.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	667174
Sequences flagged as poor quality	0
Sequence length	301
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.78512352100052	34.0	34.0	34.0	34.0	34.0
2	33.864218329850985	34.0	34.0	34.0	34.0	34.0
3	33.878258145551236	34.0	34.0	34.0	34.0	34.0
4	33.88790930102192	34.0	34.0	34.0	34.0	34.0
5	33.891097374897704	34.0	34.0	34.0	34.0	34.0
6	37.634811908137905	38.0	38.0	38.0	38.0	38.0
7	37.63642168309916	38.0	38.0	38.0	38.0	38.0
8	37.630285352846485	38.0	38.0	38.0	38.0	38.0
9	37.581645867494835	38.0	38.0	38.0	37.0	38.0
10-14	37.6626100537491	38.0	38.0	38.0	38.0	38.0
15-19	37.6189590721461	38.0	38.0	38.0	37.2	38.0
20-24	37.6231315968548	38.0	38.0	38.0	37.6	38.0
25-29	37.529584785977875	38.0	38.0	38.0	37.0	38.0
30-34	37.476443626400304	38.0	38.0	38.0	37.0	38.0
35-39	37.40261371096595	38.0	38.0	38.0	37.0	38.0
40-44	37.38709542038509	38.0	38.0	38.0	37.0	38.0
45-49	37.37667984663671	38.0	38.0	38.0	37.0	38.0
50-54	37.327033127789754	38.0	38.0	38.0	37.0	38.0
55-59	37.28688797824856	38.0	38.0	38.0	37.0	38.0
60-64	37.12265256140077	38.0	38.0	38.0	36.6	38.0
65-69	37.05396073588	38.0	38.0	38.0	36.6	38.0
70-74	37.11625213212746	38.0	38.0	38.0	37.0	38.0
75-79	37.00114033220719	38.0	38.0	38.0	36.2	38.0
80-84	36.88701777946982	38.0	38.0	38.0	35.8	38.0
85-89	36.943973835910874	38.0	38.0	38.0	36.0	38.0
90-94	36.82325810058545	38.0	38.0	38.0	35.8	38.0
95-99	36.829359057757046	38.0	38.0	38.0	36.0	38.0
100-104	36.81671617898779	38.0	38.0	38.0	35.8	38.0
105-109	36.68059786502472	38.0	38.0	38.0	35.0	38.0
110-114	36.5827331400804	38.0	38.0	38.0	34.8	38.0
115-119	36.664023777905015	38.0	38.0	38.0	35.0	38.0
120-124	36.523712254973965	38.0	37.8	38.0	34.6	38.0
125-129	36.454085740751296	38.0	37.0	38.0	34.0	38.0
130-134	36.374091316508135	38.0	37.0	38.0	34.0	38.0
135-139	36.209045316514135	38.0	37.0	38.0	33.6	38.0
140-144	36.10360895358632	38.0	37.0	38.0	33.2	38.0
145-149	35.671092998228346	38.0	37.0	38.0	30.4	38.0
150-154	35.39556457535815	38.0	36.4	38.0	28.4	38.0
155-159	35.42164023178361	38.0	36.0	38.0	29.0	38.0
160-164	35.374325138569546	38.0	36.0	38.0	28.6	38.0
165-169	35.11566847628954	38.0	35.6	38.0	28.0	38.0
170-174	34.98262821992464	38.0	35.2	38.0	28.0	38.0
175-179	34.65720396778052	38.0	35.0	38.0	25.0	38.0
180-184	34.22634784928668	37.6	35.0	38.0	23.0	38.0
185-189	33.53212415351918	37.0	33.8	37.8	22.2	38.0
190-194	32.54280232742883	36.8	31.0	37.0	20.2	38.0
195-199	32.29915824057891	36.8	30.4	37.2	20.0	37.8
200-204	32.07742537928637	37.0	30.0	37.0	19.2	37.4
205-209	31.122228384199623	35.6	27.8	37.0	16.4	37.2
210-214	30.369304859002302	35.0	26.2	37.0	14.6	37.0
215-219	29.571606807219705	34.2	24.4	37.0	11.2	37.0
220-224	28.277181065209373	32.4	22.0	36.2	9.2	37.0
225-229	27.39487300164575	31.0	20.6	36.0	9.0	37.0
230-234	27.013924703300784	30.8	20.2	36.0	9.0	37.0
235-239	26.03854286887679	28.8	18.8	35.6	8.8	37.0
240-244	25.092067136908813	27.6	16.8	34.2	8.0	37.0
245-249	24.341147886458405	26.0	15.6	34.0	8.0	36.6
250-254	23.361818356230906	25.0	14.0	33.0	8.0	36.0
255-259	22.85489182731941	23.8	13.6	32.2	8.0	36.0
260-264	21.930359996043013	22.2	12.0	31.2	8.0	36.0
265-269	21.023911903041785	20.8	10.4	29.8	8.0	35.8
270-274	20.146260795534598	19.6	9.0	28.8	7.8	34.6
275-279	18.892791985299187	18.0	9.0	26.8	7.0	33.8
280-284	18.52278536034078	17.8	8.8	26.2	7.0	32.8
285-289	17.392034162002716	16.0	8.0	24.2	7.0	31.2
290-294	17.00817178127445	15.4	8.0	23.4	7.0	30.6
295-299	16.0800858546646	13.6	8.0	22.0	7.0	29.8
300-301	14.226527862296791	12.0	8.0	18.5	7.0	25.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.06971458235222627
1101	2	0.03455061573023244
1101	3	-0.00496163515958159
1101	4	-0.012100628793980661
1101	5	0.030278208421691488
1101	6	0.11737775283572915
1101	7	0.11524575429771033
1101	8	0.02317553832448027
1101	9	0.1092952061453829
1101	10-14	0.02700629343667771
1101	15-19	0.03383978118450415
1101	20-24	0.03886971664520189
1101	25-29	0.017384603222097894
1101	30-34	0.04885810596199747
1101	35-39	0.043256166173968325
1101	40-44	0.056069176856198055
1101	45-49	0.08921761131198025
1101	50-54	0.05325139348767749
1101	55-59	0.07751302806336469
1101	60-64	0.1783151263160363
1101	65-69	0.1625637770197983
1101	70-74	0.1807844046814111
1101	75-79	-0.03690386546427504
1101	80-84	0.0965750793393596
1101	85-89	-0.016026834856070593
1101	90-94	0.19182651966365682
1101	95-99	-0.054860096821713
1101	100-104	-0.014781272167446957
1101	105-109	0.018517003384140196
1101	110-114	0.012500907160891472
1101	115-119	-0.04597425595482463
1101	120-124	-0.008894960730508217
1101	125-129	2.4868184812731897E-4
1101	130-134	0.004193775363638963
1101	135-139	-0.05688963299652272
1101	140-144	0.05354340672244717
1101	145-149	0.24259158104874956
1101	150-154	0.33074462108426417
1101	155-159	0.1430687630948384
1101	160-164	0.100809658181781
1101	165-169	0.0579295148319261
1101	170-174	0.19915051755526036
1101	175-179	0.2771088686125225
1101	180-184	-0.0793708143650349
1101	185-189	0.014331200261644028
1101	190-194	0.15220384224621597
1101	195-199	-0.007165733582567668
1101	200-204	-0.10741217187868202
1101	205-209	-0.23317813572705504
1101	210-214	-0.2383114396597925
1101	215-219	-0.07417711456827902
1101	220-224	0.234786454308896
1101	225-229	0.44907190022076904
1101	230-234	0.342631252644928
1101	235-239	0.40924246639299966
1101	240-244	0.5312767664702065
1101	245-249	0.36576898878216113
1101	250-254	0.30437448393213273
1101	255-259	0.10150575686660801
1101	260-264	0.5762213109323113
1101	265-269	0.34602780476600614
1101	270-274	0.22111571586420098
1101	275-279	0.2836978096218097
1101	280-284	0.016865952445144217
1101	285-289	0.3288892225116875
1101	290-294	0.2506466644954237
1101	295-299	0.3027843946226376
1101	300-301	0.11724143814290144
1102	1	-0.033354692072165903
1102	2	-4.3223319359242396E-4
1102	3	0.00868717887006909
1102	4	0.03784613473502674
1102	5	0.036132264206209186
1102	6	0.030340197814382464
1102	7	0.0938950931939786
1102	8	0.00456722378527985
1102	9	0.0038041885365913686
1102	10-14	0.041737571509706584
1102	15-19	0.019323144216812693
1102	20-24	-0.03458598559047488
1102	25-29	0.03279097060395486
1102	30-34	0.09327231895873922
1102	35-39	0.09479317774646034
1102	40-44	0.0621451110372746
1102	45-49	0.06413635478782709
1102	50-54	0.06054788705268521
1102	55-59	-0.0026238023812439337
1102	60-64	0.16118263237807184
1102	65-69	0.30352058603941856
1102	70-74	0.1345988157582454
1102	75-79	0.05474782131359035
1102	80-84	0.13184676790372407
1102	85-89	0.14029128777755062
1102	90-94	0.29644345505550973
1102	95-99	0.1088800961596732
1102	100-104	0.10101225769420807
1102	105-109	0.1446929165053561
1102	110-114	0.29417341256114327
1102	115-119	0.12621589832988178
1102	120-124	0.23911801162498847
1102	125-129	0.10894177701589314
1102	130-134	0.2754686405120381
1102	135-139	0.2542238470777818
1102	140-144	0.2583339067019139
1102	145-149	0.3467273343757853
1102	150-154	0.7718782547325773
1102	155-159	0.4704213554469092
1102	160-164	0.5980098044924276
1102	165-169	0.5901629103016433
1102	170-174	0.4644405340706399
1102	175-179	0.522034256052109
1102	180-184	0.7795609484109107
1102	185-189	0.756773870809063
1102	190-194	0.8326081264618139
1102	195-199	0.7638264860033672
1102	200-204	0.19022247270837767
1102	205-209	0.09878676275853238
1102	210-214	-0.007560058869387376
1102	215-219	0.1430447696963526
1102	220-224	0.9496974258467787
1102	225-229	1.2330305420354861
1102	230-234	0.8064436229360439
1102	235-239	0.9269912384985233
1102	240-244	0.9128470369818942
1102	245-249	1.2040707551376215
1102	250-254	0.7820649143423175
1102	255-259	0.7493152374573029
1102	260-264	1.1417259685530112
1102	265-269	1.1271381486807783
1102	270-274	1.2637681374553225
1102	275-279	1.3066818165122314
1102	280-284	0.6793799135711076
1102	285-289	1.1813747756396005
1102	290-294	1.0137192010616758
1102	295-299	1.0282554868602816
1102	300-301	0.5391864373376691
1103	1	-0.005339305565222219
1103	2	0.0342225364479134
1103	3	0.009418057466163532
1103	4	0.02991966365888743
1103	5	0.034973982639378676
1103	6	0.08072788548624743
1103	7	0.13049171651535119
1103	8	0.07303561840897999
1103	9	0.12466360028178514
1103	10-14	0.05231003778097687
1103	15-19	-0.01557303979675595
1103	20-24	0.07098781753483507
1103	25-29	-0.0013191042013218635
1103	30-34	0.09664060565831534
1103	35-39	-0.3924776298499921
1103	40-44	-0.2854591879730606
1103	45-49	-0.11960418233475423
1103	50-54	0.058488546346168846
1103	55-59	-0.2699460154308966
1103	60-64	0.2003251332123881
1103	65-69	0.2585064661284875
1103	70-74	0.1581358388087395
1103	75-79	0.25106493480175374
1103	80-84	0.26109615851295587
1103	85-89	0.2741540933755573
1103	90-94	0.2924336735774702
1103	95-99	0.2913891662752306
1103	100-104	0.27082433699990105
1103	105-109	0.16267370093617473
1103	110-114	0.3465355458289068
1103	115-119	0.12759256525026785
1103	120-124	0.4334091569673717
1103	125-129	0.3527634621545701
1103	130-134	0.35933739852584523
1103	135-139	0.4648236359133904
1103	140-144	0.5251955393812509
1103	145-149	0.7849574567223243
1103	150-154	0.9763154391813629
1103	155-159	0.1284185724485809
1103	160-164	-0.3555916022293175
1103	165-169	0.6106751303185476
1103	170-174	0.9084204949213017
1103	175-179	0.6532042277732515
1103	180-184	0.6570122215448322
1103	185-189	0.889574414137762
1103	190-194	0.6925489535938993
1103	195-199	0.8573963156471862
1103	200-204	0.6518627633423932
1103	205-209	1.010732773371398
1103	210-214	1.0450969629320639
1103	215-219	0.7526982219999319
1103	220-224	1.3109797908879486
1103	225-229	1.8371995750313879
1103	230-234	1.1524193019505589
1103	235-239	1.457156325335486
1103	240-244	1.3968738755465218
1103	245-249	1.7698611481336748
1103	250-254	1.6358923177033837
1103	255-259	0.7155802624281726
1103	260-264	1.707518703483153
1103	265-269	0.9105917988777961
1103	270-274	1.1401901305583237
1103	275-279	1.7083498590651764
1103	280-284	0.7190431044172669
1103	285-289	0.8177065652480486
1103	290-294	1.3941665885366206
1103	295-299	1.4449571849576586
1103	300-301	-0.8404269288608042
2116	1	0.02111518587197736
2116	2	0.030990600763047382
2116	3	0.019804679538019343
2116	4	0.06273947618222309
2116	5	-0.0015811103397638249
2116	6	-0.32317964930890497
2116	7	-0.15913857867645476
2116	8	0.029319097818230944
2116	9	-0.02601753626300507
2116	10-14	0.07197463938584292
2116	15-19	0.09486812199230599
2116	20-24	-0.1588742666399483
2116	25-29	-0.216146973451103
2116	30-34	-0.02396779949314265
2116	35-39	-0.20747345189579391
2116	40-44	-0.25503533508070575
2116	45-49	0.02941289896537569
2116	50-54	0.11707813449265103
2116	55-59	0.07474390589260338
2116	60-64	0.33775096770376933
2116	65-69	0.2011982884773218
2116	70-74	-0.7977769374490578
2116	75-79	0.2182435172249555
2116	80-84	-0.13341218311047243
2116	85-89	0.0639547489435941
2116	90-94	0.22260087841856802
2116	95-99	0.12914682952281709
2116	100-104	0.20005297076874484
2116	105-109	0.15353722291786198
2116	110-114	0.2886931421778556
2116	115-119	0.19010888463150621
2116	120-124	-0.31214800685176414
2116	125-129	-0.09993692796972198
2116	130-134	0.11858823660451634
2116	135-139	0.3933136240713111
2116	140-144	0.35444397951003737
2116	145-149	0.5619745675554455
2116	150-154	0.7606870334585096
2116	155-159	-0.4799680245844513
2116	160-164	-0.524689314996003
2116	165-169	0.5548823157577587
2116	170-174	0.7833241034346301
2116	175-179	0.5577854677155898
2116	180-184	0.5249425789164022
2116	185-189	0.820125932983828
2116	190-194	0.573549518251717
2116	195-199	0.7573160308721683
2116	200-204	0.6603832860376926
2116	205-209	0.6246692601211983
2116	210-214	0.617209736557573
2116	215-219	0.7345025011367099
2116	220-224	1.2229069111876747
2116	225-229	0.9500103735743828
2116	230-234	0.772760658304211
2116	235-239	1.0689727932483777
2116	240-244	1.5123177322203851
2116	245-249	1.0061980180418466
2116	250-254	1.0079923793053318
2116	255-259	1.005511280439766
2116	260-264	0.3368525966591456
2116	265-269	0.5814769312995232
2116	270-274	0.9641112697867626
2116	275-279	1.6460630623313897
2116	280-284	-0.06570676886375892
2116	285-289	-1.200433171349836
2116	290-294	0.11018993371845909
2116	295-299	0.8284434317359128
2116	300-301	1.4370126641489946
1117	1	-0.10606472487305751
1117	2	0.030373199441712018
1117	3	0.020119887513388335
1117	4	0.039774369038489965
1117	5	6.929580746515285E-4
1117	6	-0.642176786407866
1117	7	-0.27059554739150826
1117	8	-0.12216103058854344
1117	9	0.07666217592957736
1117	10-14	0.09236113578729288
1117	15-19	0.06972734972960382
1117	20-24	0.02619395382534151
1117	25-29	0.09693282432060357
1117	30-34	0.0521543869265102
1117	35-39	-0.13529158736918845
1117	40-44	-0.04051684938679756
1117	45-49	0.14184101297733775
1117	50-54	0.1088636813340429
1117	55-59	0.23196405723686553
1117	60-64	0.2253549776063153
1117	65-69	0.24155893972938003
1117	70-74	-0.06390909434453818
1117	75-79	0.18719657917046106
1117	80-84	0.011452513945457099
1117	85-89	0.13965900704464218
1117	90-94	0.26079411773125827
1117	95-99	0.24731483950027666
1117	100-104	0.24716656404126525
1117	105-109	0.33092020949596446
1117	110-114	0.2855965491856338
1117	115-119	0.2421483564153064
1117	120-124	0.385545197702335
1117	125-129	0.2657546678492153
1117	130-134	0.2046499176946739
1117	135-139	0.26234391673222035
1117	140-144	0.37882806217388776
1117	145-149	0.6703038986257113
1117	150-154	0.9592902780373649
1117	155-159	-0.020260142702689166
1117	160-164	-0.5883128227179668
1117	165-169	0.21069463337438776
1117	170-174	0.6167824301151654
1117	175-179	0.5901609772303829
1117	180-184	0.37887945669411494
1117	185-189	0.7736381211842556
1117	190-194	0.9316747259699625
1117	195-199	0.7253557832640851
1117	200-204	0.8730489329076292
1117	205-209	1.0943869791582053
1117	210-214	0.7902990381830364
1117	215-219	0.5253208388130126
1117	220-224	1.0976669420545662
1117	225-229	0.7046782608651547
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2108	165-169	-0.20478312463549742
2108	170-174	-0.6653677691389177
2108	175-179	-0.6490701107685624
2108	180-184	-0.47976891827121904
2108	185-189	-0.9659578245637945
2108	190-194	-0.5227305808252467
2108	195-199	-1.4023279152905026
2108	200-204	-1.0096351239615764
2108	205-209	-0.22145538733844816
2108	210-214	0.16173675219812367
2108	215-219	-0.7585556890872311
2108	220-224	-0.340003140544745
2108	225-229	-0.020151882429537693
2108	230-234	-0.18149745688069885
2108	235-239	-0.7170736355615226
2108	240-244	-0.3857655293065321
2108	245-249	-0.21624730044478468
2108	250-254	-0.9001780991956636
2108	255-259	-0.8070646250364959
2108	260-264	-0.006879831966340788
2108	265-269	-0.8394175593730644
2108	270-274	-0.7554069542047372
2108	275-279	-0.35073378857305215
2108	280-284	-0.6403181398204723
2108	285-289	-0.18338226881472508
2108	290-294	-0.48140957407637686
2108	295-299	-0.04661232289917905
2108	300-301	-0.4652119569198021
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	29.0
13	173.0
14	428.0
15	785.0
16	834.0
17	1142.0
18	2205.0
19	1953.0
20	1676.0
21	1991.0
22	2978.0
23	4697.0
24	7420.0
25	11128.0
26	15948.0
27	21336.0
28	28092.0
29	38679.0
30	49351.0
31	64131.0
32	88773.0
33	116868.0
34	123835.0
35	72864.0
36	9841.0
37	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.12070014718799	22.71986018639815	17.980616750652754	31.178822915761106
2	26.473603587669782	26.155845401649348	18.51660886065704	28.85394215002383
3	40.304178520146166	25.51987937179806	21.013109024032712	13.16283308402306
4	41.22912463615189	24.989732813329056	20.49540299831828	13.285739552200775
5	40.171529466076315	24.918986651158466	21.250828119800833	13.658655762964383
6	24.477122909465894	25.592124393336668	35.55054603446777	14.380206662729664
7	16.99331808493736	32.828767308078554	36.185013204951034	13.992901402033054
8	23.93618456354714	36.575765842194095	23.65260037111758	15.83544922314119
9	14.76721215155267	21.70962897235204	39.26981567027492	24.25334320582037
10-14	22.378989588922828	33.980131120217514	28.060176205907304	15.580703084952352
15-19	22.162644227742685	33.14631565378747	23.739024602277667	20.952015516192176
20-24	21.176724512645876	30.223120205523596	28.631781214495767	19.968374067334757
25-29	17.277052163303726	28.539781226486642	34.35724413721159	19.825922472998048
30-34	17.084328825763595	30.514858192915188	32.32194300137595	20.078869979945264
35-39	18.980445880684798	31.342258541250107	31.340609795945284	18.336685782119808
40-44	18.22529055388849	29.67004109872387	33.063488685110634	19.04117966227701
45-49	19.878112756192536	29.06606072778615	32.42617368182813	18.629652834193177
50-54	18.635708226039984	31.58912067916316	31.27714809030328	18.49802300449358
55-59	20.184839337264343	31.818416185283	28.117612496889866	19.879131980562793
60-64	18.260843498097948	31.120097605722048	29.591710708151098	21.02734818802891
65-69	18.206225062727267	31.944080554697873	30.042417720114994	19.80727666245987
70-74	19.65061588131432	31.9172509720103	27.264911402422758	21.167221744252622
75-79	19.56278272234829	34.15324937722393	27.87143983428611	18.412528066141668
80-84	20.13720558654864	31.527607490699577	26.880753746398987	21.454433176352797
85-89	19.897567950789448	32.357166196524446	26.380704284039847	21.36456156864626
90-94	18.967435781370376	31.711487557968386	29.344368935240283	19.976707725420955
95-99	19.7883910344228	30.40040529157311	28.284015863927554	21.52718781007653
100-104	19.077482036170473	28.53501485369634	30.71096895262705	21.67653415750614
105-109	19.470662825589727	28.91224777943985	32.62387323247009	18.99321616250034
110-114	18.60782944179479	28.737151028067643	30.32030624694607	22.33471328319149
115-119	17.86799245773966	30.334635342504356	30.482992442751065	21.31437975700492
120-124	21.01742573901261	29.56590034983378	28.4462823791095	20.970391532044115
125-129	21.01217973122454	30.160677724251844	29.334536417786065	19.492606126737552
130-134	20.435238783285918	29.535563436225033	30.101622665151815	19.92757511533723
135-139	19.178415226013005	27.41713555983896	32.13200754226034	21.272441671887695
140-144	18.994565135931555	30.227436920503497	29.970532424824707	20.80746551874024
145-149	18.83637551823063	31.416691897466027	28.630282355127747	21.116650229175598
150-154	18.61814159424678	28.629952606066784	29.765218668593203	22.986687131093237
155-159	18.938088114944527	30.622086592103408	30.32237467287394	20.11745062007812
160-164	19.62711376642375	29.05212133566356	28.721233141579255	22.59953175633343
165-169	19.38142073881776	29.043787677577367	29.53334512436036	22.041446459244515
170-174	17.904804443818254	31.372115819861087	31.630489197720536	19.092590538600128
175-179	19.068968514960115	30.762619646449053	29.388765149721063	20.77964668886977
180-184	21.603389820346717	28.5036886929047	28.932722198407014	20.96019928834157
185-189	18.36984055134043	30.70038700548882	28.77432274039456	22.15544970277619
190-194	19.38339923318355	29.90779017167935	28.679864623021878	22.02894597211522
195-199	18.77783007131573	31.150224679019267	31.037210682670484	19.03473456699452
200-204	20.275550306216967	28.426077754828576	29.68125256679667	21.617119372157788
205-209	20.511710588242348	29.210370907739208	29.719413526306482	20.558504977711962
210-214	20.30648676357292	28.572366429147415	29.419072086142446	21.702074721137215
215-219	19.029338673269642	30.370428104212692	28.480546304262454	22.11968691825521
220-224	19.63613689981924	29.479266278362164	30.176835428239112	20.707761393579485
225-229	18.048095399401056	30.91409437418125	29.94016553402861	21.097644692389093
230-234	16.819870078869982	31.074112600311167	27.79134078965907	24.314676531159787
235-239	18.83862380728266	30.18094230290749	27.637347978188597	23.343085911621255
240-244	17.063045022737697	30.696310108007808	29.255636460653445	22.985008408601054
245-249	19.43870714386352	30.046284777284487	28.837394742600882	21.677613336251113
250-254	20.434159604540945	30.840050721401013	27.02332524948514	21.702464424572902
255-259	18.93841786400549	30.741755524046198	26.78833407776682	23.53149253418149
260-264	17.01238357609859	32.19358068509864	28.162128620120086	22.631907118682683
265-269	18.517148450029527	31.5322839319278	27.138857329572197	22.811710288470476
270-274	17.376636379715038	31.281794554344145	27.984214013136004	23.357355052804817
275-279	17.811575391127352	32.2297331730553	25.58403055274936	24.374660883067985
280-284	18.57815202630798	30.41107717027342	27.84934664720148	23.16142415621712
285-289	18.64572060661836	31.99021544604556	26.720016067772423	22.644047879563654
290-294	16.572768123458047	33.158096688420116	26.188070878061797	24.081064310060043
295-299	17.815292562360042	31.21341658997503	26.097869521294292	24.873421326370632
300-301	13.021115930776677	33.9490147997374	34.58010953664262	18.449759732843308
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.25
14	0.0
15	0.0
16	0.25
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	0.25
25	0.25
26	1.0
27	1.25
28	4.5
29	10.5
30	25.75
31	91.0
32	476.5
33	1850.25
34	6087.5
35	9581.5
36	13460.5
37	15326.25
38	17408.5
39	18474.75
40	22756.0
41	24893.0
42	22456.25
43	18711.75
44	16925.75
45	14228.0
46	9366.75
47	5105.0
48	2698.25
49	1128.25
50	463.5
51	233.75
52	119.0
53	82.75
54	63.75
55	42.25
56	27.5
57	22.5
58	19.0
59	14.75
60	14.25
61	8.5
62	3.75
63	4.75
64	4.25
65	3.0
66	2.25
67	2.25
68	1.75
69	1.0
70	0.5
71	0.5
72	0.0
73	0.25
74	0.0
75	0.0
76	0.25
77	0.25
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	667174.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.411228131962845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	49.551389892367595	4.167880446405418
2	4.387261145880828	0.7380450874500073
3	2.1677757045899533	0.5470096797069778
4	1.8556595683072556	0.6243350385716807
5	1.9600057853699715	0.8243027900356917
6	1.8430222891331525	0.9301248555714798
7	1.692779649026106	0.9966849063571802
8	1.890762455322274	1.2722907484052683
9	1.663385922870535	1.2592006621883662
>10	31.137219665583554	56.629065142048184
>50	1.5846234535425396	8.470687754351708
>100	0.185945811629236	2.887565637195078
>500	0.0124707593049793	0.6981465101044084
>1k	0.05522723182222316	11.270668586781548
>5k	0.008907618035599443	5.169606663262322
>10k+	0.003563047214239777	3.514385491564719
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT	13386	2.006373150032825	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT	10067	1.5089017257866764	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG	9743	1.4603386822627982	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA	6788	1.0174257390126114	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA	6413	0.9612185127118262	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA	6201	0.9294426941097824	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTCTATAT	5354	0.8024893056384091	No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT	4173	0.6254740142751366	No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	3786	0.5674681567327264	No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	3708	0.5557770536621631	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT	3451	0.517256367904025	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG	3304	0.4952231351941173	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG	3283	0.49207553052127334	No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	3062	0.45895073848801066	No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT	3018	0.4523557572687185	No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT	3008	0.45085689790069755	No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	2987	0.4477092932278536	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT	2987	0.4477092932278536	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT	2979	0.44651020573343686	TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT	2967	0.44471157449181176	No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT	2922	0.43796670733571746	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	2613	0.3916519528638706	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG	2305	0.34548708432882574	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC	2055	0.30801560012830237	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTAACAAGA	2024	0.30336913608743743	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA	1932	0.2895796299016448	No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT	1899	0.28463339398717574	No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	1856	0.27818829870468575	No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA	1692	0.25360700506914235	No Hit
GGGGGGAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA	1680	0.25180837382751725	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	1664	0.24941019883868376	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA	1608	0.2410165863777665	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1577	0.2363701223369016	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTCTATAT	1529	0.22917559737040114	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA	1453	0.217784266173442	No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA	1421	0.212987916195775	No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	1193	0.17881392260489767	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG	1078	0.1615770398726569	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA	864	0.12950144939700886	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG	791	0.11855977601045604	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT	685	0.10267186670943412	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	5.995437472083744E-4	0.0	0.0
2	7.494296840104681E-4	0.0	0.0
3	7.494296840104681E-4	0.0	0.0
4	7.494296840104681E-4	0.0	0.0
5	0.0011990874944167488	0.0	0.0
6	0.0013489734312188424	0.0	0.0
7	0.0014988593680209362	0.0	0.0
8	0.0020984031152293105	0.0	0.0
9	0.002248289052031404	0.0	0.0
10-14	0.003417399359087734	0.0	0.0
15-19	0.005335939350154533	0.0	0.0
20-24	0.006594981219292119	0.0	0.0
25-29	0.007464319652744261	0.0	0.0
30-34	0.01522841117909271	0.0	0.0
35-39	0.01756663179320537	0.0	0.0
40-44	0.02584033550468094	0.0	0.0
45-49	0.0390602751306256	0.0	0.0
50-54	0.04790354540194912	0.0	0.0
55-59	0.061483211276218794	0.0	0.0
60-64	0.08396610179653284	0.0	0.0
65-69	0.11460278727888078	0.0	0.0
70-74	0.16298596767859658	0.0	0.0
75-79	0.22518863145146542	0.0	0.0
80-84	0.31583964602937165	0.0	0.0
85-89	0.43484907985023397	0.0	0.0
90-94	0.5803583472977064	0.0	0.0
95-99	0.7669063842415922	0.0	0.0
100-104	1.0148776780869757	0.0	0.0
105-109	1.3009799542548122	0.0	0.0
110-114	1.6186182315258086	0.0	0.0
115-119	1.968332099272454	0.0	0.0
120-124	2.3581854208946993	0.0	0.0
125-129	2.770131929601573	0.0	0.0
130-134	3.2130148956643994	0.0	0.0
135-139	3.659255306711592	0.0	0.0
140-144	4.295700971560642	0.0	0.0
145-149	5.0942932428421965	0.0	0.0
150-154	5.740181721709779	0.0	0.0
155-159	6.888457883550617	0.0	0.0
160-164	7.433652990074553	0.0	0.0
165-169	8.00753626490241	0.0	0.0
170-174	8.54517712021152	0.0	0.0
175-179	9.096637458893783	0.0	0.0
180-184	9.648967136009498	0.0	0.0
185-189	10.282924694307631	0.0	0.0
190-194	10.932470390033185	0.0	0.0
195-199	11.623954170875965	0.0	0.0
200-204	12.332854697575147	0.0	0.0
205-209	13.096133842146127	0.0	0.0
210-214	13.954140898776032	0.0	0.0
215-219	14.75920824252744	0.0	5.995437472083744E-5
220-224	15.61940363383465	0.0	1.498859368020936E-4
225-229	16.475582082035572	0.0	1.498859368020936E-4
230-234	17.47472173675833	0.0	1.498859368020936E-4
235-239	18.45473594594514	0.0	1.498859368020936E-4
240-244	19.46601636154886	0.0	1.498859368020936E-4
245-249	20.474418967165985	0.0	1.498859368020936E-4
250-254	21.58024743170447	0.0	1.498859368020936E-4
255-259	22.607865414419628	0.0	1.498859368020936E-4
260-264	23.695287885918816	0.0	1.498859368020936E-4
265-269	24.690890232533043	0.0	1.498859368020936E-4
270-274	25.67525113388711	0.0	1.498859368020936E-4
275-279	26.63383165411122	0.0	1.498859368020936E-4
280-284	27.55790843168349	0.0	1.498859368020936E-4
285-289	28.417834028304462	0.0	1.498859368020936E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTT	9400	0.0	292.17554	1
CGGGTTA	9555	0.0	287.12717	2
GGGGGGA	6555	0.0	262.59726	1
GTTATTA	10465	0.0	261.45483	5
TTATTAG	10515	0.0	259.93103	6
TATTAGT	11015	0.0	248.3999	7
ATTAGTA	11075	0.0	247.05417	8
GGGTTAT	11320	0.0	242.48895	3
GGGGAGC	7220	0.0	238.00209	3
GAGCAGA	7295	0.0	235.15079	6
GGAGCAG	7315	0.0	234.50786	5
CAGAAGC	7355	0.0	232.8314	9
GGGAGCA	7455	0.0	230.49966	4
TTAGTAG	12105	0.0	226.27634	9
GGTTATT	12415	0.0	220.86388	4
GGGGGAG	7850	0.0	219.27707	2
GCAGAAG	8905	0.0	192.96744	8
GGCGTGG	80	0.0	165.93748	295
CCGGTTA	45	1.78552E-8	163.8889	1
CCGCCAA	905	0.0	151.57458	295
>>END_MODULE