##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_619_flu.3510000003ba43.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	199758
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.8175091861152	34.0	34.0	34.0	34.0	34.0
2	33.88941118753692	34.0	34.0	34.0	34.0	34.0
3	33.89844712101643	34.0	34.0	34.0	34.0	34.0
4	33.89826690295257	34.0	34.0	34.0	34.0	34.0
5	33.892164519068075	34.0	34.0	34.0	34.0	34.0
6	37.67490163097348	38.0	38.0	38.0	38.0	38.0
7	37.68369727370118	38.0	38.0	38.0	38.0	38.0
8	37.689043742928945	38.0	38.0	38.0	38.0	38.0
9	37.60299962955176	38.0	38.0	38.0	38.0	38.0
10-14	37.67427987865317	38.0	38.0	38.0	38.0	38.0
15-19	37.64013656524394	38.0	38.0	38.0	37.6	38.0
20-24	37.63640705253356	38.0	38.0	38.0	37.6	38.0
25-29	37.5541735499955	38.0	38.0	38.0	37.0	38.0
30-34	37.50201143383494	38.0	38.0	38.0	37.0	38.0
35-39	37.4198680403288	38.0	38.0	38.0	37.0	38.0
40-44	37.41099930916409	38.0	38.0	38.0	37.0	38.0
45-49	37.38824277375624	38.0	38.0	38.0	37.0	38.0
50-54	37.34428758798146	38.0	38.0	38.0	37.0	38.0
55-59	37.30361237096887	38.0	38.0	38.0	37.0	38.0
60-64	37.12862663823226	38.0	38.0	38.0	36.6	38.0
65-69	37.08759999599515	38.0	38.0	38.0	36.6	38.0
70-74	37.17864916549025	38.0	38.0	38.0	37.0	38.0
75-79	37.070619449533936	38.0	38.0	38.0	37.0	38.0
80-84	36.95768379739485	38.0	38.0	38.0	36.2	38.0
85-89	37.00955456101883	38.0	38.0	38.0	36.0	38.0
90-94	36.85978834389611	38.0	38.0	38.0	35.8	38.0
95-99	36.864622192853346	38.0	38.0	38.0	36.0	38.0
100-104	36.85824447581574	38.0	38.0	38.0	36.0	38.0
105-109	36.69272719991189	38.0	38.0	38.0	35.0	38.0
110-114	36.6090509516515	38.0	38.0	38.0	34.8	38.0
115-119	36.68678901470779	38.0	38.0	38.0	35.0	38.0
120-124	36.60631263829233	38.0	37.8	38.0	34.8	38.0
125-129	36.55228025911352	38.0	37.4	38.0	34.2	38.0
130-134	36.42277055236836	38.0	37.0	38.0	34.0	38.0
135-139	36.20477978353808	38.0	37.0	38.0	34.0	38.0
140-144	36.159816377817165	38.0	37.0	38.0	33.6	38.0
145-149	35.736895643728914	38.0	37.0	38.0	30.6	38.0
150-154	35.406546921775345	38.0	36.4	38.0	29.0	38.0
155-159	35.47341983800398	38.0	36.4	38.0	29.2	38.0
160-164	35.42288869532133	38.0	36.0	38.0	29.0	38.0
165-169	35.21485897936503	38.0	36.0	38.0	28.2	38.0
170-174	35.048203326024485	38.0	35.4	38.0	28.0	38.0
175-179	34.78745882517846	38.0	35.2	38.0	25.8	38.0
180-184	34.46875919863034	37.6	35.0	38.0	24.0	38.0
185-189	33.72445759368836	37.0	33.8	37.8	22.8	38.0
190-194	32.755115689984876	36.8	31.4	37.0	21.2	38.0
195-199	32.53320017220837	36.8	30.8	37.2	20.6	37.8
200-204	32.416963525866294	37.0	30.4	37.0	20.2	37.4
205-209	31.292368766207108	35.4	28.4	37.0	18.8	37.2
210-214	30.541481192242617	34.6	27.0	37.0	16.8	37.0
215-219	29.737053835140518	33.4	25.0	37.0	14.6	37.0
220-224	28.280619549655086	31.8	22.4	36.2	9.6	37.0
225-229	27.18116921474985	30.4	20.2	36.2	9.2	37.0
230-234	26.812306891338515	29.8	20.0	36.0	9.0	37.0
235-239	25.890389371139076	28.4	19.0	35.0	9.0	37.0
240-244	24.813997937504382	26.8	17.2	33.8	8.6	37.0
245-249	23.84807717337979	25.0	15.6	33.0	8.0	36.2
250-254	23.006868310655893	24.0	14.2	32.2	8.0	36.0
255-259	22.337857807947618	23.0	13.2	31.2	8.0	36.0
260-264	21.176957118112917	21.2	11.8	29.8	7.8	35.8
265-269	20.289053755043604	19.8	10.4	28.6	7.0	34.6
270-274	19.13174240831406	18.6	9.0	27.0	7.0	33.6
275-279	17.968067361507426	17.0	8.0	25.2	7.0	31.8
280-284	17.72310195336357	16.6	8.0	24.8	7.0	31.0
285-289	16.475414251244004	14.6	8.0	22.6	7.0	29.8
290-294	15.706179477167371	13.2	8.0	21.2	7.0	28.4
295-299	15.004749747194106	13.0	8.0	20.2	7.0	27.0
300-301	13.244716106488852	11.0	8.0	16.5	7.0	23.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.06153223355460824
1101	2	0.04052206313874507
1101	3	0.020473599636993356
1101	4	-0.02031704255196587
1101	5	0.008154470192785368
1101	6	0.09590497615146631
1101	7	0.0909822196286143
1101	8	0.01696125092048817
1101	9	-0.0749008261295927
1101	10-14	0.046461930419717135
1101	15-19	0.09422061803392268
1101	20-24	-0.00641262553957489
1101	25-29	0.08255817362120155
1101	30-34	0.08501808825267432
1101	35-39	0.1191528209474626
1101	40-44	0.0033957586848387677
1101	45-49	0.012609915714897113
1101	50-54	-0.03901673223911217
1101	55-59	-0.014485128101362932
1101	60-64	0.15949984641288495
1101	65-69	0.15313090430881715
1101	70-74	0.06840791279820024
1101	75-79	0.06006909358875134
1101	80-84	0.11675788573356982
1101	85-89	0.07561717632913201
1101	90-94	0.1401723226905034
1101	95-99	-0.08359730982812863
1101	100-104	-0.2104528113430888
1101	105-109	0.04212886363141166
1101	110-114	0.10476665202559587
1101	115-119	-0.11394340283395366
1101	120-124	0.0945814724612859
1101	125-129	0.11880503429354405
1101	130-134	0.015220611179756816
1101	135-139	0.033134083423014715
1101	140-144	0.13405945837311606
1101	145-149	0.2681376908966655
1101	150-154	0.6185283856899062
1101	155-159	0.11967808184898843
1101	160-164	0.13343348729171822
1101	165-169	0.12909895151319262
1101	170-174	0.28075513148577613
1101	175-179	0.4611706995553888
1101	180-184	-0.01081496740810195
1101	185-189	0.023948219657505376
1101	190-194	0.18416053759566609
1101	195-199	-0.277123251830524
1101	200-204	-0.5522326699253419
1101	205-209	-0.3973901523371417
1101	210-214	-0.3273239428924235
1101	215-219	-0.34024546099611896
1101	220-224	0.12955856659593223
1101	225-229	0.6206037026945417
1101	230-234	0.004292432769375409
1101	235-239	0.4687924751713197
1101	240-244	0.484517245819017
1101	245-249	0.6796999555426844
1101	250-254	0.081719812172377
1101	255-259	-0.35251907601892185
1101	260-264	-0.059032400849275746
1101	265-269	-0.5570174182570256
1101	270-274	0.004395025131259445
1101	275-279	0.16863938647126986
1101	280-284	0.1412799401738951
1101	285-289	0.359102978635196
1101	290-294	0.14269274834252776
1101	295-299	0.35359026750007594
1101	300-301	0.2991407811622615
1102	1	0.01644508213536966
1102	2	0.052594263573240596
1102	3	0.07982946680399294
1102	4	0.08568061565981111
1102	5	0.0010268286274595084
1102	6	0.03785049430737786
1102	7	0.19189072060201084
1102	8	0.0298763540438145
1102	9	0.1208994729421562
1102	10-14	0.08421832801585794
1102	15-19	0.1117240742876291
1102	20-24	0.03659092946423925
1102	25-29	0.036331838378700354
1102	30-34	0.020239429561257793
1102	35-39	-0.007147153659651906
1102	40-44	-0.08087476017642814
1102	45-49	0.08282758916239885
1102	50-54	0.0795482876519813
1102	55-59	0.02171242853887634
1102	60-64	0.22322752183539762
1102	65-69	0.2796685845277622
1102	70-74	0.18102112274091553
1102	75-79	0.049760993258651354
1102	80-84	0.12941412250810913
1102	85-89	0.1360734018179457
1102	90-94	0.2751192797765327
1102	95-99	0.20852666387612828
1102	100-104	0.2614718282190296
1102	105-109	0.024716398546630103
1102	110-114	0.24246521027406231
1102	115-119	0.15947730083107814
1102	120-124	0.006591601400714353
1102	125-129	0.04327389979589924
1102	130-134	0.20140476601992674
1102	135-139	0.1436100589329854
1102	140-144	0.22536451155710324
1102	145-149	0.18495483391502887
1102	150-154	0.7324542666819198
1102	155-159	0.4093114368132973
1102	160-164	0.43716694664899336
1102	165-169	0.608542000916465
1102	170-174	0.6393167656589469
1102	175-179	0.4321409898807005
1102	180-184	0.6368636997476216
1102	185-189	0.6240434429738144
1102	190-194	0.7994449379003754
1102	195-199	0.9040041922345949
1102	200-204	0.43236231356927846
1102	205-209	0.36794291766257814
1102	210-214	-0.09184937809824234
1102	215-219	0.11083162051503948
1102	220-224	1.1735665512473403
1102	225-229	1.362401095691748
1102	230-234	1.3244106507531797
1102	235-239	1.316869669539745
1102	240-244	1.3735355286957542
1102	245-249	1.1287808742008103
1102	250-254	1.4164777333415763
1102	255-259	0.8224463614440509
1102	260-264	1.3020713431904483
1102	265-269	1.5504748933418853
1102	270-274	1.2762519503351086
1102	275-279	1.4580942858872348
1102	280-284	1.0988801715312846
1102	285-289	1.2349712742083696
1102	290-294	0.9004100691110839
1102	295-299	0.8833140493469838
1102	300-301	0.44022470464503627
1103	1	-0.07272626340535737
1103	2	-0.009851071189615368
1103	3	0.05965270411460466
1103	4	0.04871280819430268
1103	5	0.0921096940733861
1103	6	0.07911393137534617
1103	7	0.07978818977787228
1103	8	0.07293140017421962
1103	9	0.1545767858107041
1103	10-14	0.12071566176300053
1103	15-19	0.0270564389294492
1103	20-24	0.04358737446042227
1103	25-29	0.033304442277916735
1103	30-34	0.16449570019297255
1103	35-39	-0.4521158357689643
1103	40-44	-0.34660424131515555
1103	45-49	-0.1426139648821234
1103	50-54	0.0908340140295465
1103	55-59	-0.32941050123569227
1103	60-64	0.16808193596511956
1103	65-69	0.16059359087597613
1103	70-74	0.09825865906685749
1103	75-79	0.1481287950812913
1103	80-84	0.1928772887186483
1103	85-89	0.007333594239582908
1103	90-94	0.22226187492931615
1103	95-99	0.1656564215151448
1103	100-104	0.1544725617912377
1103	105-109	0.04809901288514595
1103	110-114	0.19693083113007503
1103	115-119	0.03531032850932547
1103	120-124	0.2878650545508421
1103	125-129	0.2792527954875723
1103	130-134	0.27753404401558157
1103	135-139	0.3823878147662896
1103	140-144	0.2889102046417804
1103	145-149	0.7177645565683051
1103	150-154	0.9136776394212447
1103	155-159	-0.031068186807722498
1103	160-164	-0.5620889007679821
1103	165-169	0.5996213395728986
1103	170-174	0.8826208031275584
1103	175-179	0.6563199532867259
1103	180-184	0.5145581669202599
1103	185-189	0.7329034435381061
1103	190-194	0.5244590450583502
1103	195-199	0.6766080914530619
1103	200-204	0.6119464345522729
1103	205-209	0.6111919372150965
1103	210-214	0.9704372511374295
1103	215-219	0.5026649867650796
1103	220-224	1.0377675218198128
1103	225-229	1.7441111653811063
1103	230-234	1.1016804924708694
1103	235-239	1.203867102036991
1103	240-244	1.159890380147374
1103	245-249	0.7979835376322342
1103	250-254	0.9671675733663996
1103	255-259	-0.00774295661593527
1103	260-264	1.224549688702961
1103	265-269	0.5023109399519328
1103	270-274	0.5614845773700665
1103	275-279	1.2100572969190324
1103	280-284	0.2084441192783757
1103	285-289	0.5333567099784808
1103	290-294	0.9792599125216324
1103	295-299	0.8909037003358975
1103	300-301	-0.8771651889869929
2116	1	0.02723935953560641
2116	2	0.03415872627221006
2116	3	0.023760905907941776
2116	4	0.056483455915390834
2116	5	0.07408322373792231
2116	6	-0.5195768756261145
2116	7	-0.15560156012902837
2116	8	0.06370688933112234
2116	9	0.12023911714825886
2116	10-14	0.09263605022376709
2116	15-19	0.07241911690234559
2116	20-24	-0.1209634319680788
2116	25-29	-0.11530471920831786
2116	30-34	0.10623589556925594
2116	35-39	-0.15931997820457156
2116	40-44	-0.11206532361956789
2116	45-49	0.034649191504847465
2116	50-54	0.17200970944880112
2116	55-59	0.24650407177258415
2116	60-64	0.3608376869606289
2116	65-69	0.23853712655650128
2116	70-74	-0.8556431371344786
2116	75-79	0.2339668791332059
2116	80-84	-0.1814523586300183
2116	85-89	0.2057844780164686
2116	90-94	0.22917810949545014
2116	95-99	0.26309962774885065
2116	100-104	0.1924871433937838
2116	105-109	0.19930793946147674
2116	110-114	0.47554758421684795
2116	115-119	0.35904625540077717
2116	120-124	-0.14929010057564085
2116	125-129	0.0991341946338764
2116	130-134	0.13383077533021748
2116	135-139	0.5365689245723502
2116	140-144	0.5295367265428297
2116	145-149	0.6807354506583181
2116	150-154	1.1212434571655336
2116	155-159	-0.19744813925279203
2116	160-164	-0.6122958793109703
2116	165-169	0.577407313732806
2116	170-174	0.8336442368228063
2116	175-179	0.6478581834411372
2116	180-184	0.4346279237025641
2116	185-189	0.7993679062533161
2116	190-194	0.6837716471152433
2116	195-199	0.7457575457172538
2116	200-204	0.9724070871434378
2116	205-209	0.8528204071494976
2116	210-214	0.5281985884050293
2116	215-219	0.9419307500790239
2116	220-224	0.9105301482271209
2116	225-229	0.6608456312475539
2116	230-234	0.9933075300278063
2116	235-239	0.7502546460826025
2116	240-244	1.5849175953191228
2116	245-249	0.7773300870349367
2116	250-254	0.9194779982095547
2116	255-259	0.8381446876960936
2116	260-264	-0.1688327274313366
2116	265-269	0.22574964852041646
2116	270-274	0.36243854075850024
2116	275-279	1.0907739153623233
2116	280-284	-0.6171632087647119
2116	285-289	-1.4080440119397597
2116	290-294	0.06533791979810921
2116	295-299	0.6652870618393205
2116	300-301	0.8785213495296382
1117	1	0.03097251027840997
1117	2	-0.008413760588915409
1117	3	0.03988083292028932
1117	4	0.01916651688047466
1117	5	-0.00890562779922277
1117	6	-0.40162752264487267
1117	7	-0.19949612866785316
1117	8	-0.03576609904693839
1117	9	0.04768123510125122
1117	10-14	0.09502745195813844
1117	15-19	0.10959984146137458
1117	20-24	0.050876592506810425
1117	25-29	0.07874045009461383
1117	30-34	0.051835330315597616
1117	35-39	-0.0670532454945203
1117	40-44	-0.12254946774468323
1117	45-49	0.13353871573755072
1117	50-54	0.16426005271656408
1117	55-59	0.26389290582766023
1117	60-64	0.1440505275423476
1117	65-69	0.19445535416579673
1117	70-74	-0.05223413313909475
1117	75-79	0.16739442140885075
1117	80-84	-0.08669661595155276
1117	85-89	-0.0035283672999710802
1117	90-94	0.08245545163780577
1117	95-99	0.20759332145567555
1117	100-104	0.05428918078356304
1117	105-109	0.11881611050898044
1117	110-114	0.25434183115839915
1117	115-119	0.23128727489692835
1117	120-124	0.35933620921395715
1117	125-129	0.025770680446079552
1117	130-134	0.015064135493673803
1117	135-139	0.2609400252508678
1117	140-144	0.10974483603887109
1117	145-149	0.5279822064592707
1117	150-154	0.6622261681249739
1117	155-159	-0.501372500155604
1117	160-164	-0.8697448808297281
1117	165-169	-0.03017772015691378
1117	170-174	0.26950870706140506
1117	175-179	0.41297052396276257
1117	180-184	0.42426178356191
1117	185-189	0.8593110166780988
1117	190-194	0.7229431425972237
1117	195-199	0.6298834603391796
1117	200-204	0.7542614250646054
1117	205-209	0.8833229802919398
1117	210-214	0.2706956838316401
1117	215-219	0.21263553251828426
1117	220-224	0.7594100208993666
1117	225-229	0.47164663792351647
1117	230-234	1.1225583139894582
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2108	165-169	0.14973598978551905
2108	170-174	-0.43041249407358606
2108	175-179	-0.5201656355289686
2108	180-184	-0.1877827819665896
2108	185-189	-0.8603961200962402
2108	190-194	-0.5445666365892805
2108	195-199	-1.5461885399152422
2108	200-204	-0.9519269360250107
2108	205-209	-0.3296756522120674
2108	210-214	0.042889931867854614
2108	215-219	-0.5185960266038308
2108	220-224	-0.1891113519676786
2108	225-229	0.03411505653983227
2108	230-234	-0.21468669394342754
2108	235-239	-0.4246453635679437
2108	240-244	-0.23899716536528004
2108	245-249	0.464173537354295
2108	250-254	-0.4849433802454257
2108	255-259	-0.8024676571356792
2108	260-264	-0.08872750076867675
2108	265-269	-0.9702292918499502
2108	270-274	-0.9135119483810641
2108	275-279	-0.2622522447580593
2108	280-284	-0.7304334203975493
2108	285-289	-0.1877271166981913
2108	290-294	-0.3672387394698102
2108	295-299	-0.3241495251567521
2108	300-301	-0.601400159386392
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	13.0
14	58.0
15	127.0
16	209.0
17	294.0
18	630.0
19	589.0
20	553.0
21	640.0
22	976.0
23	1391.0
24	2100.0
25	3047.0
26	4252.0
27	5963.0
28	8100.0
29	11448.0
30	16251.0
31	23024.0
32	31598.0
33	38085.0
34	34163.0
35	14945.0
36	1301.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.11702159613132	22.553289480271125	15.451195947095986	33.87849297650157
2	44.60246898747485	23.530972476696803	17.434095255258864	14.432463280569488
3	43.88960642377276	22.145295807927592	19.319376445499053	14.64572132280059
4	44.33614673755244	21.883979615334557	18.835290701749116	14.94458294536389
5	27.564853472701966	22.075211005316433	35.97653160323992	14.383403918741678
6	26.44349663092342	21.746313038776922	36.34697984561319	15.46321048468647
7	17.040619149170496	48.31345928573574	19.863534877201413	14.782386687892348
8	25.65704502447962	20.88877541825609	37.378728261196045	16.075451296068245
9	15.704001842229099	19.478068462840035	38.79544248540734	26.022487209523526
10-14	26.518287127424184	34.21740305769982	23.443666836872616	15.820642978003384
15-19	21.16941499214049	36.476736851590424	21.149791247409368	21.20405690885972
20-24	26.731545169655284	30.512820512820515	25.611489902782363	17.144144414741838
25-29	19.066270186926182	26.591175322139787	36.992661119955144	17.349893370978883
30-34	19.024619789945834	29.01721082509837	30.83350854533986	21.124660839615935
35-39	21.378367825068334	30.394777680994	28.33138097097488	19.895473522962785
40-44	20.011013326124612	31.00001001211466	28.04243134192373	20.946545319837004
45-49	21.183031468076372	29.637160964767368	27.11741206860301	22.06239549855325
50-54	22.939456742658617	30.915407643248333	25.53179347009882	20.613342143994235
55-59	21.610548764004445	28.169685319236276	28.73907427987865	21.480691636880625
60-64	20.847525505862095	27.35379809569579	29.18391253416634	22.614763864275776
65-69	19.010602829423604	28.62673835340762	29.07127624425555	23.291382572913225
70-74	20.678821373862373	26.43158221447952	30.244295597673183	22.64530081398492
75-79	21.59272719991189	26.264379899678612	28.61172018141952	23.53117271898998
80-84	22.711881376465524	27.96423672643899	25.804523473402817	23.51935842369267
85-89	21.86315441684438	27.525405740946546	27.543327426185684	23.068112416023386
90-94	21.974188768409775	29.856426275793712	27.90596621912514	20.263418736671372
95-99	23.686260375053816	28.452127073759247	27.603500235284695	20.258112315902242
100-104	22.343435557023998	27.66016880425315	27.889446229938226	22.10694940878463
105-109	24.29990288248781	28.492275653540784	24.9563972406612	22.251424223310206
110-114	21.473082429739986	27.264890517526208	29.762212276854992	21.499814775878814
115-119	24.612280859840407	27.092481903102755	27.694009751799676	20.601227485257162
120-124	20.39768119424504	28.28492475895834	27.08377136334965	24.23362268344697
125-129	20.16519989186916	25.581053074219806	29.523923947977053	24.72982308593398
130-134	23.603960792558993	29.089998898667385	26.900850028534528	20.40519028023909
135-139	23.016550025530893	26.98074670351125	25.571942049880352	24.430761221077503
140-144	21.704662641796574	28.157270297059444	27.100491594829744	23.037575466314237
145-149	22.087025300613742	26.106689093803503	29.058560858638955	22.747724746943803
150-154	21.763233512550187	28.12142692658116	25.67756985953003	24.437769701338617
155-159	21.083410927221937	27.720141371058983	30.429519718859822	20.766927982859258
160-164	22.757736861602538	30.5819041039658	25.275983940568086	21.384375093863575
165-169	20.551967881136175	28.614523573523964	27.376926080557475	23.456582464782386
170-174	21.35754262657816	30.558375634517766	26.79442124971215	21.289660489191924
175-179	19.33779873647113	28.77461728691717	28.53933259243685	23.34825138417485
180-184	20.793660328998087	29.50900589713553	27.318455330950446	22.378878442915926
185-189	20.997406862303386	28.140750307872526	28.194815727029702	22.667027102794382
190-194	20.294956897846394	27.15205398532224	28.091791067191302	24.461198049640064
195-199	19.520519828993084	31.694049800258313	26.852691757026005	21.932738613722606
200-204	20.81688843500636	27.954224611780255	26.550325894332143	24.678561058881247
205-209	21.083811411808288	27.30624055106679	28.629741987805247	22.980206049319676
210-214	22.476596681985203	27.073859369837507	28.451926831466075	21.997617116711222
215-219	21.54567026101583	28.297540023428347	28.65046706514883	21.506322650406993
220-224	20.055467115209403	27.699416293715394	27.52520549865337	24.71991109242183
225-229	22.51163908329078	27.875229027122817	27.36260875659548	22.250523132990917
230-234	21.104136004565525	28.222749526927583	27.757286316442897	22.915828152063998
235-239	21.75011764234724	27.886742958980363	25.986443596752068	24.376695801920324
240-244	22.02815406642037	30.60653390602629	25.261166010873154	22.104146016680183
245-249	22.15190380360236	28.755293905625805	24.935171557584678	24.15763073318716
250-254	20.390973077423684	28.187807246768593	24.878803352055986	26.542416323751738
255-259	21.20095315331551	29.482373672143293	24.73162526657255	24.58504790796864
260-264	19.598814565624405	28.899268114418447	27.01729092201564	24.484626397941508
265-269	20.024830044353667	29.01661009821884	25.193884600366445	25.764675257061043
270-274	18.84800608736571	31.183031468076372	24.799607525105376	25.169354919452537
275-279	19.854023368275612	29.55115689984882	24.52647703721503	26.06834269466054
280-284	17.376825959410887	30.723475405240343	26.75247048929204	25.147228146056726
285-289	19.094003744530884	29.71445448993282	25.759569078585088	25.43197268695121
290-294	19.68862323411328	30.842519448532723	25.00405490643679	24.464802410917212
295-299	18.742178035422864	31.026842479400074	23.986824057109104	26.244155428067963
300-301	15.32954875399233	33.896264480020825	26.93158722053685	23.842599545449993
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.25
30	0.0
31	0.75
32	3.5
33	21.25
34	68.5
35	135.75
36	256.5
37	547.5
38	1269.25
39	2895.0
40	3661.75
41	4341.0
42	5899.75
43	8302.25
44	8212.75
45	7069.5
46	6772.5
47	5787.5
48	4251.5
49	3303.5
50	2381.5
51	1000.75
52	220.0
53	45.25
54	17.75
55	13.75
56	10.75
57	8.75
58	4.25
59	4.75
60	3.5
61	3.5
62	1.75
63	1.0
64	0.75
65	1.75
66	0.75
67	0.25
68	0.0
69	0.0
70	0.25
71	0.25
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.25
90	0.25
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	199758.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.71083516575546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.29809248745896	9.617485841526117
2	17.191015252243943	7.464625969469125
3	10.118879658117967	6.590679849585454
4	6.8254803815499425	5.927475179637139
5	5.019994323853939	5.449413464911045
6	3.8870346287098205	5.063446086450144
7	2.4675623942210176	3.7500988281504584
8	1.8977559142025358	3.296149267047105
9	1.4122295080879783	2.7594613859682613
>10	6.708099376062812	23.94718847850904
>50	0.06316187111151512	0.91100773292468
>100	0.05765744012128361	2.5479722425427958
>500	0.023059462720994583	3.6952252729945503
>1k	0.025365408993094044	12.412372333471602
>5k	0.004611892544198917	6.567398066812426
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	7071	3.5397831375964914	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	6047	3.0271628670691535	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATACTCCTC	3639	1.8217042621572102	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	3618	1.8111915417655364	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	3107	1.555382012234804	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	2869	1.4362378477958329	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	2455	1.2289870743599756	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	2210	1.1063386697904465	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA	1966	0.9841908709538543	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	1537	0.769431011523944	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	1259	0.6302626177674987	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	1099	0.5501657004976022	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	1034	0.5176263278567067	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	908	0.4545500055066631	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	892	0.4465403137796734	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	841	0.42100942139989384	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	821	0.4109973067411568	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	761	0.38096096276494557	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT	744	0.3724506653050191	TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	686	0.34341553279468157	No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	622	0.3113767658867229	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	572	0.2863464792398802	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	534	0.26732346138827984	No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	398	0.1992410817088677	No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	374	0.18722654411838324	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	359	0.17971745812433046	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	311	0.15568838294336146	No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG	289	0.1446750568187507	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	288	0.14417445108581384	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	278	0.1391683937564453	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	245	0.12264840456952912	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	219	0.10963265551317093	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	218	0.10913204978023408	No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	214	0.10712962684848668	No Hit
GGGGGGAGCAAAATTAAACAGAGAAAAAATAGATGGGGTAAAGCTGGAAT	214	0.10712962684848668	No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	200	0.10012114658737072	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	5.006057329368536E-4	0.0	0.0
2	5.006057329368536E-4	0.0	0.0
3	5.006057329368536E-4	0.0	0.0
4	5.006057329368536E-4	0.0	0.0
5	0.0010012114658737071	0.0	0.0
6	0.0010012114658737071	0.0	0.0
7	0.0010012114658737071	0.0	0.0
8	0.0010012114658737071	0.0	0.0
9	0.0010012114658737071	0.0	0.0
10-14	0.001101332612461078	0.0	0.0
15-19	0.0016019383453979314	0.0	0.0
20-24	0.002202665224922156	0.0	0.0
25-29	0.002503028664684268	0.0	0.0
30-34	0.005206299622543277	0.0	0.0
35-39	0.005506663062305389	0.0	0.0
40-44	0.007108601407703322	0.0	0.0
45-49	0.009411387779212847	0.0	0.0
50-54	0.01361647593588242	0.0	0.0
55-59	0.020524835050410993	0.0	0.0
60-64	0.029635859389861734	0.0	0.0
65-69	0.04245136615304519	0.0	0.0
70-74	0.0608736571251214	0.0	0.0
75-79	0.08450224771974088	0.0	0.0
80-84	0.12034561819801962	0.0	0.0
85-89	0.1655002553089238	0.0	0.0
90-94	0.23238118122928744	0.0	0.0
95-99	0.3076722834629902	0.0	0.0
100-104	0.39367634838154164	0.0	0.0
105-109	0.4905936182781165	0.0	0.0
110-114	0.6048318465343065	0.0	0.0
115-119	0.7469037535417856	0.0	0.0
120-124	0.9157080066880926	0.0	0.0
125-129	1.1123459385856886	0.0	0.0
130-134	1.356341172819111	0.0	0.0
135-139	1.637481352436448	0.0	0.0
140-144	1.9424503649415794	0.0	0.0
145-149	2.2808598404068925	0.0	0.0
150-154	2.6847485457403457	0.0	0.0
155-159	3.162626778401866	0.0	0.0
160-164	3.707686300423513	0.0	0.0
165-169	4.338349402777361	0.0	0.0
170-174	5.046806636029596	0.0	0.0
175-179	5.8374633306300625	0.0	0.0
180-184	6.68558956337168	0.0	0.0
185-189	7.632435246648446	0.0	0.0
190-194	8.730163497832375	0.0	0.0
195-199	9.901280549464852	0.0	0.0
200-204	11.165410146276994	0.0	0.0
205-209	12.565003654421853	0.0	0.0
210-214	14.068923397310746	0.0	0.0
215-219	15.527488260795565	0.0	0.0
220-224	17.109302255729432	0.0	0.0
225-229	18.816768289630453	0.0	0.0
230-234	20.57289320077294	0.0	0.0
235-239	22.450665305019076	0.0	0.0
240-244	24.22030657095085	0.0	0.0
245-249	25.999158982368662	0.0	0.0
250-254	27.768800248300444	0.0	0.0
255-259	29.41339020214459	0.0	0.0
260-264	31.029946234944283	0.0	0.0
265-269	32.52535568037325	0.0	0.0
270-274	33.85626608195917	0.0	0.0
275-279	34.99003794591455	0.0	0.0
280-284	36.05302415923267	0.0	0.0
285-289	37.017491164308815	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGCC	10	8.4598013E-4	295.0	295
CGGGTTA	3200	0.0	293.6172	1
GGGGGGA	2095	0.0	292.18378	1
GGGTTAT	3260	0.0	288.21323	2
TTATTAG	3320	0.0	281.6717	5
TAGTAGA	3345	0.0	279.5665	9
GGTTATT	3370	0.0	278.36795	3
ATTAGTA	3380	0.0	276.67157	7
GGAGCGA	1555	0.0	276.02896	5
TTAGTAG	3430	0.0	272.6385	8
GTTATTA	3460	0.0	270.27457	4
CGAAAGC	1590	0.0	269.95282	9
GAGCGAA	1600	0.0	268.26562	6
GCGAAAG	1615	0.0	265.774	8
TATTAGT	3525	0.0	265.2908	6
GGGGGAG	2330	0.0	261.4485	2
GGGAGCG	1695	0.0	253.23007	4
GGGGAGC	2435	0.0	249.56879	3
AGCGAAA	1885	0.0	227.70558	7
TCGAACC	385	0.0	180.06493	295
>>END_MODULE