##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_6019_flu.3510000003ba1c.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	322786
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.813678412322716	34.0	34.0	34.0	34.0	34.0
2	33.89331941286177	34.0	34.0	34.0	34.0	34.0
3	33.899754016593036	34.0	34.0	34.0	34.0	34.0
4	33.90113573698983	34.0	34.0	34.0	34.0	34.0
5	33.8910237742653	34.0	34.0	34.0	34.0	34.0
6	37.66842737913044	38.0	38.0	38.0	38.0	38.0
7	37.67711424906904	38.0	38.0	38.0	38.0	38.0
8	37.679902474085	38.0	38.0	38.0	38.0	38.0
9	37.60471333948808	38.0	38.0	38.0	38.0	38.0
10-14	37.67633416567013	38.0	38.0	38.0	38.0	38.0
15-19	37.64852255054433	38.0	38.0	38.0	37.8	38.0
20-24	37.6421523857912	38.0	38.0	38.0	37.8	38.0
25-29	37.56501768973871	38.0	38.0	38.0	37.0	38.0
30-34	37.51747101794997	38.0	38.0	38.0	37.0	38.0
35-39	37.44910126213652	38.0	38.0	38.0	37.0	38.0
40-44	37.46422087698971	38.0	38.0	38.0	37.0	38.0
45-49	37.43398350610001	38.0	38.0	38.0	37.0	38.0
50-54	37.39933826126288	38.0	38.0	38.0	37.0	38.0
55-59	37.35669514786886	38.0	38.0	38.0	37.0	38.0
60-64	37.19168117576351	38.0	38.0	38.0	36.6	38.0
65-69	37.121149616154355	38.0	38.0	38.0	36.6	38.0
70-74	37.207419776570234	38.0	38.0	38.0	37.0	38.0
75-79	37.16093324989312	38.0	38.0	38.0	37.0	38.0
80-84	37.06799365523908	38.0	38.0	38.0	36.4	38.0
85-89	37.1089625944124	38.0	38.0	38.0	36.8	38.0
90-94	36.954853680147224	38.0	38.0	38.0	35.8	38.0
95-99	36.9582627499334	38.0	38.0	38.0	36.0	38.0
100-104	36.93793225232817	38.0	38.0	38.0	36.0	38.0
105-109	36.80288922072209	38.0	38.0	38.0	36.0	38.0
110-114	36.698428680302115	38.0	38.0	38.0	35.0	38.0
115-119	36.795587788813634	38.0	38.0	38.0	35.4	38.0
120-124	36.71822941515431	38.0	37.8	38.0	35.0	38.0
125-129	36.62811893948312	38.0	37.4	38.0	34.6	38.0
130-134	36.49741190757963	38.0	37.0	38.0	34.0	38.0
135-139	36.25514303594332	38.0	37.0	38.0	34.0	38.0
140-144	36.233363900540915	38.0	37.0	38.0	34.0	38.0
145-149	35.81922450168223	38.0	37.0	38.0	30.8	38.0
150-154	35.42275191613019	38.0	36.4	38.0	28.8	38.0
155-159	35.55746469797327	38.0	36.4	38.0	29.6	38.0
160-164	35.495164598216775	38.0	36.0	38.0	29.4	38.0
165-169	35.30352121839237	38.0	36.0	38.0	28.4	38.0
170-174	35.158475894245726	38.0	35.6	38.0	28.0	38.0
175-179	34.82368008525773	38.0	35.2	38.0	26.0	38.0
180-184	34.53516075666231	37.6	35.0	38.0	24.4	38.0
185-189	33.753378399310996	37.0	33.8	37.8	22.8	38.0
190-194	32.773358200169774	36.8	31.2	37.0	21.2	38.0
195-199	32.532783949737606	36.8	30.6	37.2	20.8	37.8
200-204	32.32437342387836	37.0	30.2	37.0	20.0	37.4
205-209	31.23532433252991	35.4	28.2	37.0	18.2	37.2
210-214	30.549908608180033	34.6	26.8	37.0	16.8	37.0
215-219	29.667128685878566	33.4	24.8	37.0	14.6	37.0
220-224	28.16121517042251	31.6	22.2	36.2	9.6	37.0
225-229	27.14697167782989	30.2	20.2	36.2	9.2	37.0
230-234	26.755825841269445	29.6	20.0	35.8	9.0	37.0
235-239	25.610449028148686	28.2	18.4	34.6	8.8	37.0
240-244	24.566284783107072	26.4	16.6	33.8	8.0	37.0
245-249	23.689425811528384	25.0	15.4	33.0	8.0	36.2
250-254	22.75470001796856	23.8	14.0	31.8	8.0	36.0
255-259	22.20882256355604	22.4	13.2	31.2	8.0	36.0
260-264	20.924836888836566	20.6	11.8	29.4	7.8	35.2
265-269	20.14260965469382	19.6	10.4	28.6	7.0	34.0
270-274	19.152284175893623	18.6	9.0	27.0	7.0	33.4
275-279	17.846180441530922	17.0	8.0	25.0	7.0	31.8
280-284	17.655105859609773	16.8	8.0	24.8	7.0	31.0
285-289	16.521259286338314	14.6	8.0	22.6	7.0	29.6
290-294	15.816044066347363	13.4	8.0	21.4	7.0	28.6
295-299	14.99877999665413	13.0	8.0	20.2	7.0	27.0
300-301	13.213437075957446	11.0	8.0	16.5	7.0	23.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.11281947419077909
1101	2	0.021907041777865288
1101	3	0.038115609220746194
1101	4	0.012638149249340813
1101	5	0.014653153341313896
1101	6	0.07161076890938034
1101	7	0.10163835527529841
1101	8	0.058485559895366634
1101	9	0.15169949684619155
1101	10-14	0.02227114452895762
1101	15-19	0.06095267630021084
1101	20-24	0.05924555150804878
1101	25-29	0.05215544166446051
1101	30-34	0.13180430750287542
1101	35-39	0.18114765850545922
1101	40-44	0.15117328922595874
1101	45-49	0.1091884918224153
1101	50-54	0.05853877233303706
1101	55-59	0.09466067611678142
1101	60-64	0.30225985812744227
1101	65-69	0.2955476527206642
1101	70-74	0.18112518460722526
1101	75-79	0.1812063142048217
1101	80-84	0.2359817761331371
1101	85-89	0.20254161964305695
1101	90-94	0.38305984274128235
1101	95-99	0.20778246954220236
1101	100-104	0.18185147108440702
1101	105-109	0.2251691491243335
1101	110-114	0.2576511192192683
1101	115-119	0.1793775177418624
1101	120-124	0.27584794991003037
1101	125-129	0.2022774365376847
1101	130-134	0.17510005953030117
1101	135-139	0.23774067793222287
1101	140-144	0.20217636146038842
1101	145-149	0.4528907241379656
1101	150-154	0.7151113400015561
1101	155-159	0.2674676307473973
1101	160-164	0.19856264212285168
1101	165-169	0.3025270304847325
1101	170-174	0.18673175463780467
1101	175-179	0.3620127460563154
1101	180-184	0.026290475376868017
1101	185-189	0.05252598721907731
1101	190-194	0.25810061220918357
1101	195-199	0.10236961258614485
1101	200-204	-0.17195397360242026
1101	205-209	-0.1380980739591493
1101	210-214	-0.3608703242647451
1101	215-219	-0.324301414202278
1101	220-224	-0.04114254058790223
1101	225-229	0.6483679872838124
1101	230-234	0.2359858124425216
1101	235-239	0.21107415804869945
1101	240-244	0.5601465888430432
1101	245-249	0.3652341332033231
1101	250-254	0.4734841020552949
1101	255-259	-0.2409227713302613
1101	260-264	0.2694196579754262
1101	265-269	-0.13291252368342654
1101	270-274	-0.10270842843943129
1101	275-279	0.5105648436355068
1101	280-284	0.1707732568551492
1101	285-289	0.530050817567119
1101	290-294	-0.09949711785277238
1101	295-299	-0.1229381986403979
1101	300-301	0.21556870358251246
1102	1	-0.016008701689010252
1102	2	-0.0066400879173826866
1102	3	-0.03797227171476436
1102	4	-0.012266173925709722
1102	5	-0.010563005411349025
1102	6	0.07139815374283387
1102	7	0.14097216108677912
1102	8	0.05970455351690873
1102	9	0.011954635046052431
1102	10-14	0.10395789151690593
1102	15-19	0.06470604270700164
1102	20-24	0.019520533790114314
1102	25-29	-0.02647531666296743
1102	30-34	0.066097716432715
1102	35-39	0.048977566372222725
1102	40-44	0.005710496880098503
1102	45-49	0.10660592626607723
1102	50-54	0.04079249309845068
1102	55-59	0.07023544578368046
1102	60-64	0.1515950813733724
1102	65-69	0.2875561573273302
1102	70-74	0.20340158432372846
1102	75-79	-0.009019057871718417
1102	80-84	0.1292376230502157
1102	85-89	0.14677974862959076
1102	90-94	0.295156228283453
1102	95-99	0.15175695572222025
1102	100-104	0.22524764401141084
1102	105-109	0.2150486671966263
1102	110-114	0.38577868831919204
1102	115-119	0.14022230867027474
1102	120-124	0.11558714197240505
1102	125-129	0.1914454025192569
1102	130-134	0.320925715093729
1102	135-139	0.5136783108167009
1102	140-144	0.3388879135510976
1102	145-149	0.5229800225079089
1102	150-154	0.9072615880525845
1102	155-159	0.5924314861690902
1102	160-164	0.5819843288698436
1102	165-169	0.6731833026321468
1102	170-174	0.668146354212567
1102	175-179	0.5286236603015269
1102	180-184	0.5400509289558926
1102	185-189	0.9278541232927822
1102	190-194	0.9751495136974881
1102	195-199	0.9150783298079759
1102	200-204	0.441432277283468
1102	205-209	0.18714926835126633
1102	210-214	-0.2166605439670839
1102	215-219	-0.13254237805769975
1102	220-224	0.8882465451668793
1102	225-229	1.4572893196721886
1102	230-234	1.0415053021661187
1102	235-239	1.342921075128782
1102	240-244	1.2461678503596936
1102	245-249	1.43743540889432
1102	250-254	1.1300482409638661
1102	255-259	0.5641403045024838
1102	260-264	0.9983154765438194
1102	265-269	1.1954560092719255
1102	270-274	1.4542667664578062
1102	275-279	1.4502359988445797
1102	280-284	0.28905532630943753
1102	285-289	1.0242903639880936
1102	290-294	0.7138721238198009
1102	295-299	0.8966535802398319
1102	300-301	0.4335275979836464
1103	1	-0.02788133912476809
1103	2	0.04433325315548586
1103	3	0.019381939489633737
1103	4	-0.002539842041180407
1103	5	0.022992131705862562
1103	6	0.12244369902619923
1103	7	0.12069551985675986
1103	8	0.14456007430747775
1103	9	0.16844381149331866
1103	10-14	0.11381050998136288
1103	15-19	0.06716211847896147
1103	20-24	0.12375623098548516
1103	25-29	0.15153637758759686
1103	30-34	0.08497331282533338
1103	35-39	-0.34205438753034656
1103	40-44	-0.16659791420253356
1103	45-49	-0.03292998471038544
1103	50-54	0.18960395516293005
1103	55-59	-0.15735618985404187
1103	60-64	0.26501260575998487
1103	65-69	0.3580407749989618
1103	70-74	0.24715988426851254
1103	75-79	0.2917284680722432
1103	80-84	0.3152883358898251
1103	85-89	0.31240544766545497
1103	90-94	0.462340688804737
1103	95-99	0.4127355351397739
1103	100-104	0.3215213404422599
1103	105-109	0.1414558706284481
1103	110-114	0.42575065609629803
1103	115-119	0.23954783639810984
1103	120-124	0.4619711267953548
1103	125-129	0.4252616074093254
1103	130-134	0.4991050363741465
1103	135-139	0.48674019407240365
1103	140-144	0.42210170880261444
1103	145-149	0.8313998829131037
1103	150-154	1.0582791011130581
1103	155-159	0.10594471652882476
1103	160-164	-0.46857249301227455
1103	165-169	0.5205855083999822
1103	170-174	0.835652194259012
1103	175-179	0.6085293700972301
1103	180-184	0.772246927993173
1103	185-189	0.7739994094904574
1103	190-194	0.5693961123128659
1103	195-199	0.5861799395371392
1103	200-204	0.4999666733872772
1103	205-209	1.0527976196884588
1103	210-214	0.8285013491990014
1103	215-219	0.5339970581831501
1103	220-224	1.2451680974158315
1103	225-229	1.9131855721320896
1103	230-234	1.169711947785025
1103	235-239	1.1714952543368042
1103	240-244	1.2215215802026869
1103	245-249	1.4730292531314362
1103	250-254	1.4999971317089908
1103	255-259	0.44534031016555886
1103	260-264	1.8872937161768526
1103	265-269	1.2515207382229825
1103	270-274	1.2036787976613574
1103	275-279	1.767478647464916
1103	280-284	1.1855456353717777
1103	285-289	0.7637747409790236
1103	290-294	1.1574772375768276
1103	295-299	1.2395963973366548
1103	300-301	-0.7702659546050867
2116	1	0.04264173241060121
2116	2	0.006496509695416819
2116	3	0.01892914068687901
2116	4	0.011185023179358211
2116	5	0.017992193264618095
2116	6	-0.32730726163229207
2116	7	-0.15102890487672482
2116	8	-0.02854039367581862
2116	9	0.02858506830411045
2116	10-14	0.02960525395852187
2116	15-19	0.06858727193124281
2116	20-24	-0.18186353566581914
2116	25-29	-0.21983688867861417
2116	30-34	-0.041279031537044375
2116	35-39	-0.15180283111995863
2116	40-44	-0.12520581095893846
2116	45-49	0.05414144669470744
2116	50-54	0.11421774240768912
2116	55-59	0.10677106439316475
2116	60-64	0.33779337863692405
2116	65-69	0.2571714286571165
2116	70-74	-0.723228989192684
2116	75-79	0.10331245677826217
2116	80-84	-0.14677847860903626
2116	85-89	0.03845204900688515
2116	90-94	0.17492480199622662
2116	95-99	0.1332021778213246
2116	100-104	0.1559277567791355
2116	105-109	0.2284753190894122
2116	110-114	0.3455141610599881
2116	115-119	0.23181578385155888
2116	120-124	-0.23251361963048112
2116	125-129	-0.08964833213998702
2116	130-134	0.1191677168609857
2116	135-139	0.32631727178280556
2116	140-144	0.3544560300216375
2116	145-149	0.614010220131938
2116	150-154	0.8214231937302401
2116	155-159	-0.3827116474830987
2116	160-164	-0.6440310714976363
2116	165-169	0.654692202024826
2116	170-174	0.7197720888157093
2116	175-179	0.6417226686749515
2116	180-184	0.7176530721035732
2116	185-189	1.1065014717085688
2116	190-194	0.9074745764631089
2116	195-199	0.8948065091142539
2116	200-204	0.6450893736549901
2116	205-209	0.6428915856998252
2116	210-214	0.7131665174801007
2116	215-219	1.0213423576254321
2116	220-224	1.395240668341767
2116	225-229	1.005571575587588
2116	230-234	0.9996743695883126
2116	235-239	1.1715691248363456
2116	240-244	1.558133851734226
2116	245-249	0.9969021466252208
2116	250-254	0.948761510806925
2116	255-259	0.6178536444747174
2116	260-264	0.29770535269569365
2116	265-269	0.8558818336404137
2116	270-274	0.8726347202286533
2116	275-279	1.1740139979188733
2116	280-284	0.3835109135010448
2116	285-289	-0.9222291249104551
2116	290-294	0.01105769965047898
2116	295-299	0.7714185773645337
2116	300-301	1.2900650399517524
1117	1	0.0372149658147336
1117	2	0.0738260388849028
1117	3	0.033701778114533454
1117	4	0.028251870147535385
1117	5	0.03852420996067707
1117	6	-0.401415818774872
1117	7	-0.2907848435124123
1117	8	-0.014116726920995859
1117	9	0.06387472759423218
1117	10-14	0.0716856644352788
1117	15-19	0.09624183458647195
1117	20-24	0.023641749331432038
1117	25-29	0.11971130335339097
1117	30-34	0.12523205237130952
1117	35-39	-0.06707220648971202
1117	40-44	-0.09741356846794247
1117	45-49	0.1242103956659193
1117	50-54	0.13552458305076698
1117	55-59	0.22994707920260993
1117	60-64	0.2179628475199209
1117	65-69	0.26595349374560584
1117	70-74	-0.05941097739857071
1117	75-79	0.1713906371143139
1117	80-84	0.00267911736470694
1117	85-89	0.11633139784941449
1117	90-94	0.25949075609023
1117	95-99	0.2514127216430424
1117	100-104	0.2191304351290455
1117	105-109	0.19032137394869153
1117	110-114	0.3478277275675268
1117	115-119	0.3091430501843391
1117	120-124	0.4395052029751838
1117	125-129	0.40513237523448
1117	130-134	0.14689507261750379
1117	135-139	0.2571720045612409
1117	140-144	0.3965860597659372
1117	145-149	0.6777679799583254
1117	150-154	0.9564966547832086
1117	155-159	-0.16323962920576918
1117	160-164	-0.6367160464017232
1117	165-169	0.2531957176317192
1117	170-174	0.7339509308328758
1117	175-179	0.6153413037088811
1117	180-184	0.3284400827608138
1117	185-189	0.9826144291532373
1117	190-194	0.99328782807914
1117	195-199	0.8429906347655063
1117	200-204	0.7861465912136651
1117	205-209	1.3434112248423382
1117	210-214	0.6637446364736554
1117	215-219	0.36732387853777126
1117	220-224	1.097500798715032
1117	225-229	0.47440903977038573
1117	230-234	0.7758028671332511
1117	235-239	0.8081156238153326
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2108	185-189	-1.1280539024026695
2108	190-194	-0.8772548865120484
2108	195-199	-1.7075364312959422
2108	200-204	-1.203410158822507
2108	205-209	-0.854283832473083
2108	210-214	-0.07079359792203377
2108	215-219	-1.0942236109986112
2108	220-224	-0.3406751829354562
2108	225-229	-0.12298858829835879
2108	230-234	-0.3584963520479896
2108	235-239	-0.5534104293789497
2108	240-244	-0.25092569544824883
2108	245-249	0.003000185161862845
2108	250-254	-0.7634699967467
2108	255-259	-0.918683438984047
2108	260-264	0.024407947112596418
2108	265-269	-0.9726551539162962
2108	270-274	-0.7321675750271268
2108	275-279	-0.1390832920489693
2108	280-284	-0.8309303371682688
2108	285-289	-0.08347617126045037
2108	290-294	-0.9271352925734639
2108	295-299	-0.3920382121011592
2108	300-301	-0.1175972676114565
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	28.0
15	81.0
16	142.0
17	256.0
18	544.0
19	656.0
20	753.0
21	1033.0
22	1512.0
23	2352.0
24	3643.0
25	5127.0
26	7403.0
27	10117.0
28	13745.0
29	19252.0
30	26662.0
31	37445.0
32	50972.0
33	61729.0
34	54441.0
35	22990.0
36	1901.0
37	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.268884028427504	23.624940362964935	15.099167869734128	33.00700773887343
2	43.6419175552843	23.69619500226156	18.06614908948963	14.595738352964501
3	42.885998773180994	22.520183651087716	19.427732305614246	15.166085270117044
4	43.32282069234725	22.188075071409543	19.32673659948077	15.162367636762436
5	27.253350517060838	22.25809049958796	35.55575520623571	14.932803777115488
6	26.171519210870358	22.123945896042578	35.887863785913886	15.816671107173175
7	17.197002320444383	47.24195512155225	20.130861910323656	15.43018064767971
8	25.897033948188586	21.020118592504012	36.818201532904155	16.26464592640325
9	15.952364724616308	19.789891754908826	38.18691021295843	26.070833307516434
10-14	26.324933547303786	33.9649179332437	23.68491818108592	16.025230338366594
15-19	21.021419764178123	35.947160037919865	21.613514836455114	21.417905361446905
20-24	25.490262898638726	30.903446865725282	26.235214662345953	17.371075573290042
25-29	19.694658380474987	25.590700959769013	37.26221087655598	17.452429783200017
30-34	19.52222215337716	28.33654495548134	31.206495944681617	20.934736946459882
35-39	20.572515536609394	30.134640287992664	27.243436828115218	22.04940734728272
40-44	20.28223033217054	28.9296933572088	29.068237160223802	21.719839150396858
45-49	21.454090326098406	30.140092816912755	26.203181054940426	22.202635802048416
50-54	21.9272830915839	30.324301549633503	27.229867466370912	20.51854789241169
55-59	21.757882931725664	27.352797209296565	29.642115829063222	21.247204029914556
60-64	22.510455843809833	25.808430353237128	28.604028675345276	23.077085127607766
65-69	20.447974819230076	27.825246448111134	28.253641731673618	23.473137000985172
70-74	20.45510028315974	26.853519049772917	29.22382011611408	23.467560550953262
75-79	20.496180131728142	26.42846963622958	30.175286412669696	22.900063819372587
80-84	23.174611042610273	25.807438984342568	27.767623131114732	23.250326841932427
85-89	20.95413060045975	28.003754809688154	26.603818009455182	24.43829658039692
90-94	20.161407248145828	29.637902511261334	29.194450812612693	21.006239427980148
95-99	22.409274256008622	30.15558295589028	26.687340838822003	20.747801949279086
100-104	22.490876308142237	28.252154678331777	27.030850160787644	22.226118852738345
105-109	23.191216471594185	27.910752015267082	24.009467572943063	24.88856394019567
110-114	21.821950146536714	28.566914302355123	28.725285483261352	20.88585006784681
115-119	23.785108399992566	27.941546411554402	26.906867088411516	21.366478100041515
120-124	18.586741680246355	29.1896178892517	27.825494290334774	24.39814614016717
125-129	20.757343874889244	25.099477672513675	30.611798529056404	23.531379923540673
130-134	25.16472213788702	29.071335188019308	25.844305515109085	19.91963715898459
135-139	24.55942946720118	27.931818604276515	24.532228783156643	22.97652314536566
140-144	20.746872540940437	29.437274231224404	26.981219755503645	22.834633472331515
145-149	21.08307051730868	27.350876432063348	28.9070157937457	22.659037256882268
150-154	23.266808349804514	25.718091862720193	25.67131164300806	25.343788144467233
155-159	21.532718271548333	26.894227135005856	30.46389868209898	21.109155911346836
160-164	25.109515282571117	29.49105599375438	25.631904729449236	19.767523994225275
165-169	20.53608272973425	25.972873668622555	28.237160223801528	25.253883377841667
170-174	22.401777028743503	29.402514359358833	25.79009002868774	22.40561858320993
175-179	20.067598966497926	29.36539998636868	28.727516063274123	21.839484983859276
180-184	19.86783813424374	30.553927369836366	26.076843481439717	23.501391014480184
185-189	21.89940084142435	28.60768434814397	27.208181271802374	22.284733538629308
190-194	20.335020725805954	28.051836201074398	26.49991015719393	25.11323291592572
195-199	21.100915157410792	30.668988122161434	26.99435539335659	21.235741327071185
200-204	21.725291679316946	28.1848655146134	27.171128859368128	22.91871394670153
205-209	21.054816503813672	28.331897913788083	28.073584356198843	22.5397012261994
210-214	22.990897994336805	27.826671540897063	27.318842824657825	21.863587640108307
215-219	22.57836461308731	27.94043112154802	27.310602070721778	22.170602194642893
220-224	19.420792723352314	27.648534942655505	27.812978258040932	25.117694075951245
225-229	21.17421449505245	29.422961342809167	26.358330286939335	23.04449387519905
230-234	20.619109874653795	27.80027634407936	28.036717825432326	23.543895955834515
235-239	21.740533976070832	27.71588606692979	25.561455577379437	24.982124379619933
240-244	22.28584882863569	32.02827879771738	23.197350566629286	22.48852180701765
245-249	20.458632034846616	30.19300713165999	24.340956547062138	25.007404286431257
250-254	20.88138890782128	27.536572218125944	26.093634792091354	25.488404081961423
255-259	20.92674403474748	28.89561505145824	26.69657296165261	23.481067952141665
260-264	20.918131517475974	29.823598297323922	25.947965525146692	23.31030466005341
265-269	20.031785765181887	29.160620349085768	26.577422812637476	24.230171073094866
270-274	18.971516732448123	31.165230214445483	24.758136970004895	25.105116083101496
275-279	20.033706542415096	29.95501663640926	24.027002410265624	25.984274410910015
280-284	17.511849956317807	30.930399707546176	26.442410761309347	25.115339574826667
285-289	19.98246516267744	28.862218311822694	27.71811664694256	23.437199878557312
290-294	19.392786552080945	31.862348428990106	25.077357754053768	23.66750726487518
295-299	19.053428587361285	32.26757046464221	22.82050646558401	25.858494482412496
300-301	14.791378808250668	35.571245345213235	26.885924420513902	22.751451426022197
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.25
29	0.5
30	0.5
31	0.0
32	2.25
33	5.25
34	16.5
35	58.25
36	167.25
37	591.75
38	1908.75
39	3178.0
40	5493.25
41	7026.0
42	10050.25
43	15394.75
44	15867.75
45	12649.25
46	9714.25
47	7649.25
48	6579.25
49	4458.75
50	3345.75
51	2524.0
52	646.75
53	125.0
54	36.75
55	24.25
56	19.5
57	18.0
58	21.25
59	39.5
60	45.5
61	22.75
62	11.5
63	3.25
64	1.5
65	2.0
66	1.0
67	0.25
68	0.5
69	0.25
70	0.0
71	0.25
72	0.0
73	0.0
74	0.25
75	0.25
76	0.0
77	0.0
78	0.25
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.25
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	9.294083386516143E-4
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	322786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.301204208975873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	34.116606474706764	3.5144213021322286
2	6.032543054016719	1.2428491579773038
3	5.195492631530777	1.6055949169088388
4	5.20336864324049	2.1440385187440807
5	5.340208828735706	2.750529083169119
6	5.1659418769553085	3.192925332373005
7	5.497556045193809	3.964201323028185
8	4.580030986149075	3.774386757740702
9	3.6880175798623958	3.419191999480998
>10	24.26217903043745	44.06771145774368
>50	0.7673108216228471	4.991171357014507
>100	0.09052690231341534	1.862660950350414
>500	0.012043425047148183	0.8647106120771331
>1k	0.03311941887930615	9.09869100276106
>5k	0.012043425047020419	8.58197368579215
>10k+	0.0030108562617551047	4.92494254270667
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	15879	4.919358336482995	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	9412	2.915863761129665	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	7498	2.322901241069935	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	5654	1.7516249155787427	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5106	1.5818529923850477	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	4563	1.4136300830891055	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	4531	1.4037163941434883	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3751	1.1620702260940685	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3508	1.0867881506632877	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2631	0.8150911129974658	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2127	0.6589505121039946	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1993	0.6174369396442225	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	1832	0.5675586921365858	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1782	0.5520685531590589	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1556	0.4820531249806373	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1062	0.3290105518826715	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	888	0.2751048682408779	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	698	0.21624234012627563	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	692	0.21438352344897238	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	510	0.15799941757077446	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	493	0.1527327703184153	No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG	433	0.134144603545383	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT	422	0.1307367729703271	TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	417	0.1291877590725744	No Hit
GGGGGGAGCAAAAGCAGAAGAAGTGTCATTCAGGGGGAGGGGAGTTTTCG	327	0.10130550891302598	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	2.478422236404305E-4	0.0	0.0
15-19	3.7176333546064575E-4	0.0	0.0
20-24	0.0019207772332133365	0.0	0.0
25-29	0.0023545011245840897	0.0	0.0
30-34	0.0030980277955053813	0.0	0.0
35-39	0.0036556727986963495	0.0	0.0
40-44	0.0039035150223367806	0.0	0.0
45-49	0.00495684447280861	0.0	0.0
50-54	0.006691740038291623	0.0	0.0
55-59	0.009294083386516144	0.0	0.0
60-64	0.014003085635684323	0.0	0.0
65-69	0.02081874678579616	0.0	0.0
70-74	0.03197164684961554	0.0	0.0
75-79	0.04925864194853556	0.0	0.0
80-84	0.08203577602498249	0.0	0.0
85-89	0.12881599573711375	0.0	0.0
90-94	0.18922753774946868	0.0	0.0
95-99	0.2741134993463161	0.0	0.0
100-104	0.3767201799334544	0.0	0.0
105-109	0.5142726140538934	0.0	0.0
110-114	0.6837347344680376	0.0	0.0
115-119	0.8750069705625398	0.0	0.0
120-124	1.0999237885162305	0.0	0.0
125-129	1.353962067747672	0.0	0.0
130-134	1.6682260073237376	0.0	0.0
135-139	2.0182411876599358	0.0	0.0
140-144	2.39477548592566	0.0	0.0
145-149	2.8438036346062097	0.0	0.0
150-154	3.3623515270179007	0.0	0.0
155-159	3.956243455416282	0.0	0.0
160-164	4.582230951776099	0.0	0.0
165-169	5.263425303451823	0.0	0.0
170-174	6.047598099050145	0.0	0.0
175-179	6.868947228194531	0.0	0.0
180-184	7.77753682006035	0.0	0.0
185-189	8.799204426462113	0.0	0.0
190-194	9.930108492933398	0.0	0.0
195-199	11.048868290446302	0.0	0.0
200-204	12.217382414355022	0.0	0.0
205-209	13.463842917598658	0.0	0.0
210-214	14.736822538771818	0.0	0.0
215-219	16.04078243790003	0.0	0.0
220-224	17.43123927307876	0.0	0.0
225-229	18.898589158141927	0.0	0.0
230-234	20.34096893917332	0.0	0.0
235-239	21.794005935821254	0.0	0.0
240-244	23.177647109849868	0.0	0.0
245-249	24.54084130042815	0.0	0.0
250-254	26.012714306072752	0.0	0.0
255-259	27.426592231385495	0.0	0.0
260-264	28.816863184896494	0.0	0.0
265-269	30.100438061130284	0.0	0.0
270-274	31.256374192189252	1.8588166773032288E-4	0.0
275-279	32.32463613663542	3.0980277955053813E-4	0.0
280-284	33.335832409088376	3.0980277955053813E-4	0.0
285-289	34.29820376348417	3.0980277955053813E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTTAT	5260	0.0	293.5979	2
CGGGTTA	5260	0.0	293.31747	1
GTTATTA	5305	0.0	290.8294	4
GGTTATT	5330	0.0	289.742	3
GGGGGGA	3310	0.0	287.87012	1
TTATTAG	5440	0.0	284.15442	5
TTAGTAG	5520	0.0	279.769	8
GGGGAGC	3405	0.0	277.67255	3
TAGTAGA	5595	0.0	276.01877	9
TATTAGT	5635	0.0	274.3212	6
GGGAGCG	2595	0.0	270.55878	4
GGAGCGA	2605	0.0	269.52017	5
ATTAGTA	5880	0.0	262.89114	7
CGAAAGC	2660	0.0	262.2838	9
GCGAAAG	2670	0.0	261.8539	8
GAGCGAA	2835	0.0	246.61375	6
AGCGAAA	2840	0.0	246.17958	7
GGGGGAG	4245	0.0	224.81154	2
GGGAGCA	1140	0.0	207.01755	4
GCGTATT	15	0.0028487514	196.66666	295
>>END_MODULE