FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_6019_flu.3510000003ba1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_6019_flu.3510000003ba1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322786
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC158794.919358336482995No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT94122.915863761129665No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG74982.322901241069935No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA56541.7516249155787427No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA51061.5818529923850477No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA45631.4136300830891055No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG45311.4037163941434883No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT37511.1620702260940685No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT35081.0867881506632877No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT26310.8150911129974658No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG21270.6589505121039946No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG19930.6174369396442225No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA18320.5675586921365858No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT17820.5520685531590589No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA15560.4820531249806373No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT10620.3290105518826715No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT8880.2751048682408779No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6980.21624234012627563No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT6920.21438352344897238No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5100.15799941757077446No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA4930.1527327703184153No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG4330.134144603545383No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT4220.1307367729703271TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA4170.1291877590725744No Hit
GGGGGGAGCAAAAGCAGAAGAAGTGTCATTCAGGGGGAGGGGAGTTTTCG3270.10130550891302598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT52600.0293.59792
CGGGTTA52600.0293.317471
GTTATTA53050.0290.82944
GGTTATT53300.0289.7423
GGGGGGA33100.0287.870121
TTATTAG54400.0284.154425
TTAGTAG55200.0279.7698
GGGGAGC34050.0277.672553
TAGTAGA55950.0276.018779
TATTAGT56350.0274.32126
GGGAGCG25950.0270.558784
GGAGCGA26050.0269.520175
ATTAGTA58800.0262.891147
CGAAAGC26600.0262.28389
GCGAAAG26700.0261.85398
GAGCGAA28350.0246.613756
AGCGAAA28400.0246.179587
GGGGGAG42450.0224.811542
GGGAGCA11400.0207.017554
GCGTATT150.0028487514196.66666295
Produced by FastQC (version 0.11.2)