##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_3522_flu.3510000003bb6b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440633
Sequences flagged as poor quality	0
Sequence length	301
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.803162722719364	34.0	34.0	34.0	34.0	34.0
2	33.87059752673994	34.0	34.0	34.0	34.0	34.0
3	33.877734985804516	34.0	34.0	34.0	34.0	34.0
4	33.89837347633972	34.0	34.0	34.0	34.0	34.0
5	33.884516139281445	34.0	34.0	34.0	34.0	34.0
6	37.63986582938636	38.0	38.0	38.0	38.0	38.0
7	37.65288573484056	38.0	38.0	38.0	38.0	38.0
8	37.65862293564032	38.0	38.0	38.0	38.0	38.0
9	37.56744274713878	38.0	38.0	38.0	37.0	38.0
10-14	37.662324882612054	38.0	38.0	38.0	38.0	38.0
15-19	37.633446882099165	38.0	38.0	38.0	37.6	38.0
20-24	37.621024299133296	38.0	38.0	38.0	37.4	38.0
25-29	37.53010101376883	38.0	38.0	38.0	37.0	38.0
30-34	37.453037788817454	38.0	38.0	38.0	37.0	38.0
35-39	37.38060608261297	38.0	38.0	38.0	37.0	38.0
40-44	37.36956560221317	38.0	38.0	38.0	37.0	38.0
45-49	37.34030860148922	38.0	38.0	38.0	37.0	38.0
50-54	37.3238731552108	38.0	38.0	38.0	37.0	38.0
55-59	37.25899558135682	38.0	38.0	38.0	37.0	38.0
60-64	37.09171215047443	38.0	38.0	38.0	36.4	38.0
65-69	37.01391588918669	38.0	38.0	38.0	36.4	38.0
70-74	37.10739277357801	38.0	38.0	38.0	36.8	38.0
75-79	37.0189599961873	38.0	38.0	38.0	36.2	38.0
80-84	36.89608767386918	38.0	38.0	38.0	35.8	38.0
85-89	36.96042375400843	38.0	38.0	38.0	36.0	38.0
90-94	36.79398274754728	38.0	38.0	38.0	35.4	38.0
95-99	36.81180846645621	38.0	38.0	38.0	35.8	38.0
100-104	36.77127632292634	38.0	38.0	38.0	35.6	38.0
105-109	36.60005174374139	38.0	38.0	38.0	35.0	38.0
110-114	36.54741383418854	38.0	37.8	38.0	34.2	38.0
115-119	36.60842742145958	38.0	38.0	38.0	34.6	38.0
120-124	36.51629542045194	38.0	37.8	38.0	34.2	38.0
125-129	36.40842742145958	38.0	37.0	38.0	34.0	38.0
130-134	36.28000308646879	38.0	37.0	38.0	34.0	38.0
135-139	35.99664755022887	38.0	37.0	38.0	32.6	38.0
140-144	35.960270792246604	38.0	37.0	38.0	32.0	38.0
145-149	35.51920033224929	38.0	36.2	38.0	29.2	38.0
150-154	35.101918830409886	38.0	35.8	38.0	27.6	38.0
155-159	35.193025488331564	38.0	35.8	38.0	28.0	38.0
160-164	35.04633561263002	38.0	35.8	38.0	27.4	38.0
165-169	34.716525997825855	38.0	35.2	38.0	25.2	38.0
170-174	34.65087453731336	38.0	35.0	38.0	24.4	38.0
175-179	34.233869002094714	38.0	34.4	38.0	23.2	38.0
180-184	33.94321124382423	37.6	34.0	38.0	22.6	38.0
185-189	33.035507100012936	37.0	32.4	37.8	21.0	38.0
190-194	31.941409290724938	36.6	29.4	37.0	19.2	38.0
195-199	31.592697324076955	36.0	28.6	37.2	18.4	37.8
200-204	31.402565400231033	36.6	28.6	37.0	17.6	37.4
205-209	30.125201698465617	34.4	25.2	37.0	14.8	37.2
210-214	29.149171759718406	33.2	23.4	37.0	11.4	37.0
215-219	28.254578299854984	32.0	21.6	36.6	9.6	37.0
220-224	26.63389169671813	29.8	19.4	36.2	9.0	37.0
225-229	25.513979661078494	28.0	17.8	34.8	8.6	37.0
230-234	25.04104912705131	27.4	17.2	34.2	8.0	37.0
235-239	23.975227456863195	25.6	15.4	33.4	8.0	37.0
240-244	22.95237487886745	24.0	13.8	32.2	8.0	36.8
245-249	22.004029203441412	22.0	13.0	31.0	8.0	36.0
250-254	21.090911938052756	20.8	11.2	30.2	7.2	36.0
255-259	20.482002482791803	20.0	10.0	29.4	7.0	35.8
260-264	19.2467227829055	18.4	8.8	27.6	7.0	33.8
265-269	18.467268225484702	17.6	8.0	26.6	7.0	32.8
270-274	17.663870840359213	16.2	8.0	24.8	7.0	32.0
275-279	16.535889958309976	14.0	8.0	23.0	7.0	30.2
280-284	16.280669854504772	13.6	8.0	22.4	7.0	30.0
285-289	15.256246808568582	13.0	8.0	20.4	7.0	27.6
290-294	14.759304001289056	12.6	8.0	19.8	7.0	26.8
295-299	14.049271389115205	12.0	8.0	19.0	7.0	25.8
300-301	12.641050488728716	10.0	8.0	16.0	7.0	22.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.059401205964348947
1101	2	0.02569874403126704
1101	3	0.059181612180964294
1101	4	0.023301972213815247
1101	5	0.003904331861960486
1101	6	0.09081658282199356
1101	7	0.06981997415033447
1101	8	0.012192886482317533
1101	9	0.035991557727285794
1101	10-14	-0.00570323988857524
1101	15-19	0.05366394003974051
1101	20-24	0.043335899414820744
1101	25-29	0.05191888026627112
1101	30-34	0.07833306753236968
1101	35-39	0.09982101763402795
1101	40-44	0.19528407671413817
1101	45-49	0.1549424000478794
1101	50-54	0.08918179667930559
1101	55-59	0.1913347745025078
1101	60-64	0.26542621076620065
1101	65-69	0.29775281463502523
1101	70-74	0.19233209036773502
1101	75-79	0.14009417972669524
1101	80-84	0.20426017320159673
1101	85-89	0.15444162819079565
1101	90-94	0.33053322527752016
1101	95-99	0.1827961624318135
1101	100-104	0.25166850474252556
1101	105-109	0.2045651829595556
1101	110-114	0.18925891666170003
1101	115-119	0.1535891631759867
1101	120-124	0.2038136114273712
1101	125-129	0.15163237021184273
1101	130-134	0.17478010282795253
1101	135-139	0.2836155074088538
1101	140-144	0.29680661115044416
1101	145-149	0.5755662807612651
1101	150-154	0.6382308726615449
1101	155-159	0.31640267013672485
1101	160-164	0.43171040287391094
1101	165-169	0.2413472063726232
1101	170-174	0.22946457585235436
1101	175-179	0.2614033542899392
1101	180-184	-0.20713976485431118
1101	185-189	0.08179725077814481
1101	190-194	0.3878475261372323
1101	195-199	0.0015484695449465846
1101	200-204	-0.48005457878348423
1101	205-209	-0.3836352991063876
1101	210-214	-0.6301700088629154
1101	215-219	-0.8631224326678577
1101	220-224	0.04921077493934689
1101	225-229	0.3116288342511133
1101	230-234	-0.135270428255037
1101	235-239	-0.14802070772265807
1101	240-244	0.016076769285376713
1101	245-249	0.1485967506185517
1101	250-254	0.2357303340019925
1101	255-259	-0.3224657734048364
1101	260-264	0.14064728293102036
1101	265-269	-0.19700572642484815
1101	270-274	0.03569766067769464
1101	275-279	0.2542490313523871
1101	280-284	-0.20498865451352444
1101	285-289	0.3564775639476654
1101	290-294	0.011669888026357711
1101	295-299	0.11710031439340618
1101	300-301	-0.16991622807669593
1102	1	-0.014682450312328399
1102	2	-0.014172842150685483
1102	3	-0.002646928242256763
1102	4	-0.007309349905916918
1102	5	0.025201130559736384
1102	6	0.03350965571039666
1102	7	0.16244070122575494
1102	8	0.0380655578514677
1102	9	0.033667448485111606
1102	10-14	0.09223671308664194
1102	15-19	0.08883485702291694
1102	20-24	0.05669997971867957
1102	25-29	0.005082200939085624
1102	30-34	0.10251827281361159
1102	35-39	0.05953344302740504
1102	40-44	0.03589720747920211
1102	45-49	0.07021640981457011
1102	50-54	0.07675278600829216
1102	55-59	-0.013376746568212639
1102	60-64	0.20382555965387894
1102	65-69	0.2469316896576359
1102	70-74	0.08163757056306054
1102	75-79	-0.012598893161715807
1102	80-84	0.10056691944866003
1102	85-89	0.11728481864115281
1102	90-94	0.24799750453235703
1102	95-99	0.13803761658265756
1102	100-104	0.14879818461230343
1102	105-109	0.1476579664647204
1102	110-114	0.214657722955792
1102	115-119	0.10381343519624409
1102	120-124	0.17052368558182707
1102	125-129	0.18952168111798073
1102	130-134	0.23882965970985026
1102	135-139	0.24941402431968385
1102	140-144	0.35733473558524764
1102	145-149	0.40371241930349555
1102	150-154	0.8480156438679103
1102	155-159	0.5793381014986352
1102	160-164	0.5569482397341048
1102	165-169	0.6990954429434311
1102	170-174	0.48046294807156187
1102	175-179	0.5002313521195632
1102	180-184	0.6761555869272016
1102	185-189	0.7677133296350789
1102	190-194	0.7798623570288932
1102	195-199	0.6772185726377344
1102	200-204	0.47785824451187153
1102	205-209	0.20705741205296135
1102	210-214	-0.17682220636698176
1102	215-219	-0.0022018321976062794
1102	220-224	0.8855573113835469
1102	225-229	1.1147921549239328
1102	230-234	0.8981967448323154
1102	235-239	1.0716754398628012
1102	240-244	1.3830997390810005
1102	245-249	1.1628615362940806
1102	250-254	0.8573852444740488
1102	255-259	0.7017519874922549
1102	260-264	1.055586693312641
1102	265-269	0.9353256173986289
1102	270-274	1.016489847329897
1102	275-279	1.1716264956316387
1102	280-284	0.6652229569894637
1102	285-289	1.1212867518659184
1102	290-294	0.8108469544036403
1102	295-299	0.6799579740063599
1102	300-301	0.32251006962632545
1103	1	-0.012933991327031436
1103	2	-0.051966227641436546
1103	3	0.005327822824597206
1103	4	0.030549232360563394
1103	5	0.06325233966357757
1103	6	0.056420395397452694
1103	7	0.01858994257236901
1103	8	0.03267793338489611
1103	9	0.17369530528928578
1103	10-14	0.07199133613073627
1103	15-19	0.05438547342879474
1103	20-24	0.06800154444129447
1103	25-29	0.10509583299219116
1103	30-34	0.15042545631290238
1103	35-39	-0.37835477413712226
1103	40-44	-0.34628332831976394
1103	45-49	-0.18611360441018832
1103	50-54	0.09729629003864204
1103	55-59	-0.46425986653028417
1103	60-64	0.1897679494120581
1103	65-69	0.28979679160168814
1103	70-74	0.07577223153983681
1103	75-79	0.24864577255663534
1103	80-84	0.1242280147545003
1103	85-89	0.18504323495220376
1103	90-94	0.2717569876997388
1103	95-99	0.27352303621281493
1103	100-104	0.2809141163334985
1103	105-109	0.04183589439264068
1103	110-114	0.16581111318812702
1103	115-119	-0.037412856885303825
1103	120-124	0.29291166566718374
1103	125-129	0.15526848054899034
1103	130-134	0.38580452931629594
1103	135-139	0.4288503685586633
1103	140-144	0.32560583563265766
1103	145-149	0.6777575828881339
1103	150-154	0.8552109971159041
1103	155-159	-0.08766995919364007
1103	160-164	-0.6384269380752841
1103	165-169	0.4380019152051702
1103	170-174	0.9227513548994466
1103	175-179	0.5472536375629034
1103	180-184	0.6678062507489244
1103	185-189	0.7203780996682667
1103	190-194	0.5191137795039928
1103	195-199	0.6421284825476476
1103	200-204	0.29740838675938264
1103	205-209	0.8326726325366032
1103	210-214	0.6878927059137148
1103	215-219	0.33335174769807807
1103	220-224	1.0225250686144634
1103	225-229	1.708161642832895
1103	230-234	0.7368631744197991
1103	235-239	0.9886532729590556
1103	240-244	1.0194226409302196
1103	245-249	1.269780692292187
1103	250-254	1.3735635280207568
1103	255-259	0.21733698037987637
1103	260-264	1.3114870015155802
1103	265-269	0.37350544196002744
1103	270-274	0.345535010914535
1103	275-279	1.2759478564661606
1103	280-284	0.3372683576532829
1103	285-289	0.5812505972902873
1103	290-294	0.7200722981684571
1103	295-299	0.8499988701656722
1103	300-301	-0.8262126662674643
2116	1	0.03019510193005459
2116	2	-0.028210923702687296
2116	3	0.05192465429770721
2116	4	0.029814175631479145
2116	5	0.007238134331970514
2116	6	-0.1607960744853969
2116	7	-0.1561719566989197
2116	8	6.755812033603092E-4
2116	9	0.12235375469431631
2116	10-14	0.13074398810011445
2116	15-19	0.11194185660378508
2116	20-24	-0.10041695938677009
2116	25-29	-0.18573930675506034
2116	30-34	0.1037110157631318
2116	35-39	-0.1660698142625776
2116	40-44	-0.08216159139512058
2116	45-49	0.06159417162523795
2116	50-54	0.1969659468968672
2116	55-59	0.19320369771656232
2116	60-64	0.3623615754613283
2116	65-69	0.23180744560038846
2116	70-74	-0.6965781051449724
2116	75-79	0.2611607619487515
2116	80-84	-0.030094118812520776
2116	85-89	0.18690999078653192
2116	90-94	0.2866454896829467
2116	95-99	0.22819569094946246
2116	100-104	0.1761486426687071
2116	105-109	0.30652031003695157
2116	110-114	0.40377693737409004
2116	115-119	0.3528907359566773
2116	120-124	-0.13857816460677697
2116	125-129	0.07740204756309765
2116	130-134	0.2442862191972992
2116	135-139	0.4966387414024922
2116	140-144	0.6382736015214121
2116	145-149	0.7385678171838563
2116	150-154	1.1681641379700665
2116	155-159	-0.42322940942885623
2116	160-164	-0.6079287137417353
2116	165-169	0.8487325904196297
2116	170-174	0.9634412128461278
2116	175-179	0.8470507042541655
2116	180-184	0.7884356899150475
2116	185-189	1.1070121740156509
2116	190-194	1.1495947083850915
2116	195-199	0.9563084474955232
2116	200-204	1.061008954050223
2116	205-209	1.3243881108135156
2116	210-214	1.0541256410673014
2116	215-219	1.2807770313434972
2116	220-224	1.5295193495848523
2116	225-229	1.0653813412001973
2116	230-234	1.3409091572627112
2116	235-239	1.1274475493173242
2116	240-244	1.7180090259500496
2116	245-249	0.9062014560593639
2116	250-254	1.1820083209365393
2116	255-259	0.782594452015335
2116	260-264	0.4879081111918069
2116	265-269	0.8991667047586631
2116	270-274	1.0291268151761344
2116	275-279	1.3515157238215707
2116	280-284	0.2785035988671929
2116	285-289	-0.7264810464695106
2116	290-294	0.26421202236364216
2116	295-299	0.6498483997293683
2116	300-301	1.6793824125998
1117	1	-0.012852444262719587
1117	2	0.08224276583479195
1117	3	0.03655452366336931
1117	4	-0.007142985719582384
1117	5	0.028513290269359004
1117	6	-0.40492047133134434
1117	7	-0.3017909987137486
1117	8	-0.03629923343709862
1117	9	0.030294792707735496
1117	10-14	0.08916048804125865
1117	15-19	0.02689595805135525
1117	20-24	-0.03025101846644418
1117	25-29	0.07240827474429068
1117	30-34	0.0032714488571627953
1117	35-39	-0.2304272345285483
1117	40-44	-0.1825752668099767
1117	45-49	0.04339245337173736
1117	50-54	0.055594286310778784
1117	55-59	0.16892512880988875
1117	60-64	0.11507782825642465
1117	65-69	0.1877907338197673
1117	70-74	-0.2855977591405079
1117	75-79	0.04341744077657239
1117	80-84	-0.10381602066022566
1117	85-89	-0.06394325153429037
1117	90-94	0.14839375377616193
1117	95-99	0.24658571095651638
1117	100-104	0.10593974541085771
1117	105-109	0.10633612738425313
1117	110-114	0.20994205447424008
1117	115-119	0.22799371347815622
1117	120-124	0.29583221552738337
1117	125-129	0.25585561941199586
1117	130-134	0.1058744267999856
1117	135-139	0.15513997713274819
1117	140-144	0.08447932118718171
1117	145-149	0.6089120563270853
1117	150-154	0.8112128610487943
1117	155-159	-0.4286625034620499
1117	160-164	-1.0516270778702506
1117	165-169	-0.03307334108561122
1117	170-174	0.47549017130205584
1117	175-179	0.43891253784249784
1117	180-184	0.4308712554087464
1117	185-189	0.6683697119702288
1117	190-194	0.9224304151051221
1117	195-199	0.6344024806837112
1117	200-204	0.6502357399746685
1117	205-209	1.2586908058823596
1117	210-214	0.6601771886723355
1117	215-219	0.08625249327123541
1117	220-224	0.850623857058082
1117	225-229	0.4677504126371801
1117	230-234	1.0395216087161643
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2108	175-179	-0.6224471669205087
2108	180-184	-0.3816734337851102
2108	185-189	-0.9059890701771636
2108	190-194	-0.44704311735942426
2108	195-199	-1.475445864374528
2108	200-204	-0.8834777878767923
2108	205-209	-0.35469404015615424
2108	210-214	0.29606578283679497
2108	215-219	-0.7581051356066304
2108	220-224	-0.43650277193511755
2108	225-229	0.11479919593783805
2108	230-234	0.09995523805534035
2108	235-239	-0.3906668707632619
2108	240-244	-0.35461017114607074
2108	245-249	-0.15972192905826432
2108	250-254	-0.5086248476458834
2108	255-259	-0.4857414115572105
2108	260-264	0.0753954124348617
2108	265-269	-0.5382629526887968
2108	270-274	-0.6990883959371352
2108	275-279	-0.23288620753094946
2108	280-284	-0.44946049076034633
2108	285-289	-0.23943377855713166
2108	290-294	-0.4885662944375273
2108	295-299	0.19362458199790744
2108	300-301	-0.017314198401605196
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	41.0
14	170.0
15	331.0
16	465.0
17	687.0
18	1490.0
19	1503.0
20	1582.0
21	2049.0
22	2983.0
23	4284.0
24	6306.0
25	9175.0
26	13186.0
27	18186.0
28	25096.0
29	33987.0
30	44723.0
31	56871.0
32	66833.0
33	70252.0
34	55804.0
35	22674.0
36	1952.0
37	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.18695603824498	18.53311032083389	14.18209711937145	30.09783652154968
2	49.92159007609507	21.071730896233372	14.652329716566847	14.354349311104706
3	49.617255176076235	19.911581747168277	16.69870391005667	13.772459166698816
4	50.200053105418796	19.597261212846064	16.20055692605865	14.002128755676493
5	34.51307550728157	19.905000306377417	31.747281751480255	13.834642434860758
6	32.540231893662074	20.901521220607624	32.17212510184213	14.386121783888179
7	16.61881883562965	50.696838412011815	18.296178452362852	14.388164299995687
8	32.00509267349472	18.6867529213654	33.5773761838083	15.730778221331585
9	15.138902442622317	17.518887600338605	35.04231412536056	32.29989583167851
10-14	27.88338594703529	35.8478824781621	20.685695351914177	15.583036222888435
15-19	23.114700896210678	35.094738705453295	18.987138956909718	22.803421441426313
20-24	24.799095846203077	30.247757203840838	24.563661822877542	20.389485127078544
25-29	19.938270624306394	23.83643531011068	37.96955743214875	18.25573663343417
30-34	22.74700260761223	26.108711785090993	28.72354090592398	22.420744701372797
35-39	22.56031663538591	29.117428789945375	26.574496236096707	21.74775833857201
40-44	23.15918235810754	27.11331198525757	27.26840704168775	22.45909861494713
45-49	22.722492414322122	28.618782524232184	25.618598697782506	23.040126363663184
50-54	22.743099132384547	26.772484130784573	28.556962370044914	21.927454366785966
55-59	21.651805470765918	24.991319306543087	29.858181298268626	23.498693924422366
60-64	23.912689244791014	26.222184902174828	26.235483951497052	23.629641901537106
65-69	19.917073846035134	29.4577573627032	27.593711773743685	23.03145701751798
70-74	22.54565590865868	25.623137622465862	28.275140536455506	23.55606593241995
75-79	21.303397612071723	25.466953224111677	27.119575701320603	26.110073462495997
80-84	28.136294830391734	24.094110064384648	26.03813150626485	21.73146359895877
85-89	22.044513234369646	30.526265622411398	23.389623564281386	24.039597578937574
90-94	22.339180224812942	28.909818374928797	27.440432287186844	21.310569113071423
95-99	22.004298361675133	28.707427723298075	26.312191778645722	22.97608213638107
100-104	21.60782329058423	28.758672182973132	24.854924619808322	24.778579906634317
105-109	26.288589370292286	26.5724537199892	23.34173790887201	23.79721900084651
110-114	20.03563055876432	27.24317062044831	29.734450211400414	22.98674860938695
115-119	24.076453647366403	26.949048300967014	24.02747865003302	24.947019401633558
120-124	19.951887398356455	27.486184647995042	27.761515819287254	24.80041213436125
125-129	22.385522645829976	23.513218483409094	30.049496973671967	24.051761897088962
130-134	25.360061547818706	27.740636765743826	23.517848186586114	23.38145349985135
135-139	25.444576325422748	27.399945079011335	23.885092582716226	23.270386012849695
140-144	23.05891751185227	27.205361377835978	25.586553889518033	24.14916722079372
145-149	20.724321600969514	25.830248755767272	28.747052535783745	24.698377107479466
150-154	22.688858982418473	25.513068698894546	25.46445681553583	26.333615503151147
155-159	23.214557239244453	26.17670487684763	27.37679656312623	23.231941320781694
160-164	27.0364679903684	27.18897585972907	23.549075988407587	22.22548016149494
165-169	22.993920110386647	25.246043759772874	26.607766554025687	25.152269575814795
170-174	23.130768689589747	28.35842980439504	24.19696209770943	24.31383940830578
175-179	23.20012345875139	28.051462328059863	24.940801074817365	23.80761313837139
180-184	21.111174151731714	28.568582017234295	25.74823038673908	24.572013444294914
185-189	22.42641835722699	26.831263205434002	26.798446779973357	23.943871657365655
190-194	21.194327251930744	27.40412088972002	25.526821640685103	25.874730217664133
195-199	20.35153971672571	29.438285375811617	27.28983076619318	22.920344141269492
200-204	22.610971034852135	28.99274452889366	24.085713053720443	24.310571382533762
205-209	21.924140951767118	27.71821447780806	26.379504031699852	23.978140538724972
210-214	22.243817417215688	29.22577292213702	25.235695011494823	23.29471464915247
215-219	22.974221177260894	27.689528473809272	26.293854522924974	23.04239582600486
220-224	18.907390050223203	29.207208720182106	26.948367462264518	24.93703376733018
225-229	22.32616364464924	29.69928702538713	23.97556423398349	23.998985095980142
230-234	20.48089907020128	29.60409229449451	26.551847001926777	23.363161633377434
235-239	20.798124516320836	29.03091688548066	24.26631686687107	25.904641731327434
240-244	21.259642378124198	31.56141278569694	23.072035004187157	24.1069098319917
245-249	19.667342209957038	30.45105564040823	23.677209832218647	26.20439231741608
250-254	19.617958709402156	28.603350180308784	24.68031218723972	27.09837892304934
255-259	20.6759820530918	29.416180812603688	25.47099286707986	24.436844267224654
260-264	20.98394809285732	30.77173066928714	24.012046306109617	24.23227493174592
265-269	19.916710732060466	30.90562894744606	23.86031005394512	25.31735026654835
270-274	19.107420461018577	32.36729886322631	23.615435067278213	24.909845608476896
275-279	19.540842379031982	32.12183381635057	21.42672019571843	26.910603608899013
280-284	17.573627032019846	32.149884370893695	24.341163734899567	25.9353248621869
285-289	18.720658688750046	30.78044540467918	24.458585716457915	26.04031019011286
290-294	18.8388976767514	32.63268978946198	23.627826331663766	24.900586202122852
295-299	18.172628922481977	33.866959578606235	21.24952057608032	26.71089092283147
300-301	13.775069048391744	37.813895010133145	24.808514114921035	23.60252182655407
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.25
29	0.75
30	1.0
31	1.75
32	5.0
33	10.0
34	30.0
35	79.0
36	203.75
37	611.75
38	1732.75
39	3612.5
40	6534.5
41	9368.25
42	14618.25
43	17463.25
44	18596.75
45	14310.0
46	9115.25
47	7398.0
48	6411.0
49	5486.75
50	5111.25
51	3038.75
52	1905.5
53	2008.5
54	2183.75
55	2546.25
56	2437.75
57	2124.25
58	2235.25
59	2628.25
60	2579.25
61	1525.5
62	549.5
63	161.75
64	62.0
65	45.75
66	59.75
67	71.5
68	82.0
69	63.25
70	27.0
71	11.25
72	2.0
73	2.25
74	1.75
75	0.25
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.25
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	4.538924683353267E-5
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	440633.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.894578901924254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	40.731229443990586	4.030183635059451
2	7.963674483850107	1.575944110593916
3	5.736561760508815	1.702825888953479
4	5.020299611253643	1.986950024593953
5	4.12947912654811	2.0429728520739765
6	4.340436939091219	2.5768077457598797
7	3.6441904366902853	2.524041086662888
8	3.271928218800423	2.5899481537882614
9	2.640447368045462	2.3513503337553647
>10	21.235405086965997	38.705383398993234
>50	1.0047531515703583	6.531179046333293
>100	0.2059163453907328	3.3906160367732987
>500	0.02522600911699596	1.674594039047038
>1k	0.027519282642148847	6.625084297442442
>5k	0.016052914874586828	11.995811435030728
>10k+	0.006879820660537212	9.696307915138899
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	15030	3.4110018995399805	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	14270	3.238522761572556	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	13432	3.048341817340054	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	9144	2.0751963652291137	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	8859	2.0105166884913297	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	8308	1.8854693134649472	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	7453	1.6914302832515948	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	7247	1.6446793590130564	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	6699	1.5203128226891767	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	5156	1.170134783368472	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	4495	1.0201233225836466	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3935	0.8930334314497553	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	3676	0.8342543568003304	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	3292	0.7471070028799477	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	2738	0.6213787891510623	No Hit
GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG	2405	0.5458056931732304	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT	1647	0.37378044767414154	TruSeq Adapter, Index 1 (97% over 36bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1515	0.34382354476400995	No Hit
GGGGGGAGCGAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC	1460	0.33134150188478845	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	1440	0.3268025772014352	No Hit
CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG	1369	0.31068939457553113	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1225	0.2780091368553876	No Hit
GGGGGGAGCGAAAGCAGGAGTGGATAGATTCTATAGGACCTGCAAGTTAG	826	0.18745758942248994	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	799	0.181330041099963	No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTGACCGGCTTAGCCAAATA	758	0.17202524549908882	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	750	0.1702096756257475	No Hit
GGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGT	720	0.1634012886007176	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	688	0.15613900910735237	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	638	0.14479169739896922	No Hit
GGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCT	583	0.13230965451974772	No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	571	0.12958629970973576	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	538	0.12209707398220287	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	509	0.11551563319134063	No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT	500	0.11347311708383168	No Hit
GGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTA	446	0.10121802043877785	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	7.262279493365227E-4	0.0	0.0
15-19	0.001089341924004784	0.0	0.0
20-24	0.0011347311708383167	0.0	0.0
25-29	0.0012255096645053822	0.0	0.0
30-34	0.004402756942852668	0.0	0.0
35-39	0.00544670962002392	0.0	0.0
40-44	0.007625393468033488	0.0	0.0
45-49	0.008714735392038273	0.0	0.0
50-54	0.01053030526537958	0.0	0.0
55-59	0.013389827815892135	0.0	0.0
60-64	0.017247913796742412	0.0	0.0
65-69	0.025145642745777096	0.0	0.0
70-74	0.04094110064384646	0.0	0.0
75-79	0.06549668318078763	0.0	0.0
80-84	0.10171730215394673	0.0	0.0
85-89	0.15414188224667696	0.0	0.0
90-94	0.2338000104395268	0.0	0.0
95-99	0.3462291748461872	0.0	0.0
100-104	0.5043199215673815	0.0	0.0
105-109	0.7085261430714449	0.0	0.0
110-114	0.9758688069209522	0.0	0.0
115-119	1.3049408464640642	0.0	0.0
120-124	1.7047747218206535	0.0	0.0
125-129	2.150724071960112	0.0	0.0
130-134	2.692490122165158	0.0	0.0
135-139	3.292490576057626	0.0	0.0
140-144	3.9412390810493085	0.0	0.0
145-149	4.7527080359392055	0.0	0.0
150-154	5.6077052785424595	0.0	0.0
155-159	6.599823435829817	0.0	0.0
160-164	7.651946177431105	0.0	0.0
165-169	8.811959158755698	0.0	0.0
170-174	10.025849176071697	0.0	0.0
175-179	11.355981054528371	0.0	0.0
180-184	12.682572571732031	0.0	0.0
185-189	14.171566814106068	0.0	0.0
190-194	15.781069506823135	0.0	0.0
195-199	17.324576234644248	0.0	0.0
200-204	18.89840297935016	0.0	0.0
205-209	20.559513245716957	0.0	0.0
210-214	22.198882062850494	0.0	0.0
215-219	23.66195904528258	0.0	0.0
220-224	25.15944107681449	0.0	0.0
225-229	26.63341147848663	0.0	0.0
230-234	28.138428124992906	0.0	0.0
235-239	29.529699318934355	0.0	0.0
240-244	30.921878297812462	0.0	0.0
245-249	32.05824348153679	0.0	0.0
250-254	33.248939593720856	0.0	0.0
255-259	34.36133925511707	0.0	0.0
260-264	35.42567170411658	0.0	0.0
265-269	36.367543965159214	0.0	0.0
270-274	37.32543863033409	0.0	0.0
275-279	38.08770564165643	0.0	0.0
280-284	38.73445701978744	0.0	1.3616774050059801E-4
285-289	39.32156692757919	0.0	2.2694623416766335E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	6690	0.0	287.7242	1
GGGTTAT	6725	0.0	286.4461	2
GGTTATT	6745	0.0	285.59674	3
GTTATTA	6770	0.0	284.32422	4
GGGGGGA	7625	0.0	277.78363	1
TTATTAG	6930	0.0	277.75977	5
TTAGTAG	7095	0.0	271.50812	8
TATTAGT	7165	0.0	268.85553	6
ATTAGTA	7310	0.0	263.52258	7
GGAGCGA	6740	0.0	261.51703	5
GGGAGCG	6750	0.0	260.6926	4
GGGGAGC	8125	0.0	259.96307	3
CGAAAGC	6825	0.0	257.1795	9
TAGTAGA	7560	0.0	255.0033	9
GAGCGAA	7170	0.0	245.83334	6
GCGAAAG	7175	0.0	245.0453	8
AGCGAAA	7300	0.0	241.25343	7
GGGGGAG	8840	0.0	239.93779	2
GGGAGCA	1525	0.0	230.19673	4
AAACGTT	185	0.0	191.35135	295
>>END_MODULE