FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_3522_flu.3510000003bb6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_3522_flu.3510000003bb6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440633
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT150303.4110018995399805No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC142703.238522761572556No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG134323.048341817340054No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT91442.0751963652291137No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT88592.0105166884913297No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA83081.8854693134649472No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA74531.6914302832515948No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG72471.6446793590130564No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC66991.5203128226891767No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG51561.170134783368472No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA44951.0201233225836466No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT39350.8930334314497553No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT36760.8342543568003304No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC32920.7471070028799477No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA27380.6213787891510623No Hit
GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG24050.5458056931732304No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT16470.37378044767414154TruSeq Adapter, Index 1 (97% over 36bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT15150.34382354476400995No Hit
GGGGGGAGCGAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC14600.33134150188478845No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA14400.3268025772014352No Hit
CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG13690.31068939457553113No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG12250.2780091368553876No Hit
GGGGGGAGCGAAAGCAGGAGTGGATAGATTCTATAGGACCTGCAAGTTAG8260.18745758942248994No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT7990.181330041099963No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTGACCGGCTTAGCCAAATA7580.17202524549908882No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA7500.1702096756257475No Hit
GGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGT7200.1634012886007176No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT6880.15613900910735237No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6380.14479169739896922No Hit
GGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCT5830.13230965451974772No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG5710.12958629970973576No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT5380.12209707398220287No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA5090.11551563319134063No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT5000.11347311708383168No Hit
GGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTA4460.10121802043877785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA66900.0287.72421
GGGTTAT67250.0286.44612
GGTTATT67450.0285.596743
GTTATTA67700.0284.324224
GGGGGGA76250.0277.783631
TTATTAG69300.0277.759775
TTAGTAG70950.0271.508128
TATTAGT71650.0268.855536
ATTAGTA73100.0263.522587
GGAGCGA67400.0261.517035
GGGAGCG67500.0260.69264
GGGGAGC81250.0259.963073
CGAAAGC68250.0257.17959
TAGTAGA75600.0255.00339
GAGCGAA71700.0245.833346
GCGAAAG71750.0245.04538
AGCGAAA73000.0241.253437
GGGGGAG88400.0239.937792
GGGAGCA15250.0230.196734
AAACGTT1850.0191.35135295
Produced by FastQC (version 0.11.2)