##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_30515_flu.3510000003ba93.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	385756
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.817980796150934	34.0	34.0	34.0	34.0	34.0
2	33.88301154097409	34.0	34.0	34.0	34.0	34.0
3	33.901637304410045	34.0	34.0	34.0	34.0	34.0
4	33.89956863924346	34.0	34.0	34.0	34.0	34.0
5	33.903187507128855	34.0	34.0	34.0	34.0	34.0
6	37.5804109333361	38.0	38.0	38.0	37.0	38.0
7	37.59506268210993	38.0	38.0	38.0	37.0	38.0
8	37.645560924522236	38.0	38.0	38.0	37.0	38.0
9	37.597063947158304	38.0	38.0	38.0	37.0	38.0
10-14	37.674113688445544	38.0	38.0	38.0	38.0	38.0
15-19	37.622804570764934	38.0	38.0	38.0	37.2	38.0
20-24	37.60974398324329	38.0	38.0	38.0	37.4	38.0
25-29	37.536040917056376	38.0	38.0	38.0	37.0	38.0
30-34	37.49712927342672	38.0	38.0	38.0	37.0	38.0
35-39	37.41877300677112	38.0	38.0	38.0	37.0	38.0
40-44	37.41864442808408	38.0	38.0	38.0	37.0	38.0
45-49	37.41308858449383	38.0	38.0	38.0	37.0	38.0
50-54	37.368492000124434	38.0	38.0	38.0	37.0	38.0
55-59	37.289725629672645	38.0	38.0	38.0	37.0	38.0
60-64	37.11627349931045	38.0	38.0	38.0	36.6	38.0
65-69	37.03462758842377	38.0	38.0	38.0	36.2	38.0
70-74	37.06110391024378	38.0	38.0	38.0	36.4	38.0
75-79	37.000836798390694	38.0	38.0	38.0	36.2	38.0
80-84	36.87454919690167	38.0	38.0	38.0	35.8	38.0
85-89	36.9040476363297	38.0	38.0	38.0	36.0	38.0
90-94	36.75368886031585	38.0	38.0	38.0	35.4	38.0
95-99	36.723819201775214	38.0	38.0	38.0	35.2	38.0
100-104	36.697659142048344	38.0	38.0	38.0	35.0	38.0
105-109	36.59121828305976	38.0	38.0	38.0	35.0	38.0
110-114	36.475248084281255	38.0	37.2	38.0	34.0	38.0
115-119	36.56911674737399	38.0	37.6	38.0	34.4	38.0
120-124	36.37661526975601	38.0	37.0	38.0	33.8	38.0
125-129	36.28710220968695	38.0	37.0	38.0	34.0	38.0
130-134	36.20414458880744	38.0	37.0	38.0	34.0	38.0
135-139	36.01690861580896	38.0	37.0	38.0	32.8	38.0
140-144	35.832926513132655	38.0	37.0	38.0	31.4	38.0
145-149	35.38304887027033	38.0	36.2	38.0	28.8	38.0
150-154	35.10906842667386	38.0	36.0	38.0	27.4	38.0
155-159	35.050475430064594	38.0	35.6	38.0	27.6	38.0
160-164	35.04657451860761	38.0	35.8	38.0	27.4	38.0
165-169	34.74265338711517	38.0	35.2	38.0	25.4	38.0
170-174	34.61919036904157	38.0	35.0	38.0	24.6	38.0
175-179	34.326551498874935	38.0	34.8	38.0	23.6	38.0
180-184	33.85967658312508	37.4	34.0	38.0	22.8	38.0
185-189	33.113286637148875	37.0	32.8	37.8	21.4	38.0
190-194	32.10928877321415	36.8	29.8	37.0	19.6	38.0
195-199	31.760090316158397	35.8	29.0	37.2	18.8	37.8
200-204	31.64877798401062	36.8	28.8	37.0	18.6	37.4
205-209	30.613033109011912	35.2	26.4	37.0	15.6	37.2
210-214	29.685664513319303	34.2	24.4	37.0	14.2	37.0
215-219	29.01426082808822	33.0	23.4	36.8	12.4	37.0
220-224	27.522004583208037	31.2	20.8	36.2	9.2	37.0
225-229	26.51496127085515	29.2	19.6	35.8	9.0	37.0
230-234	26.0173736766246	28.6	19.0	35.6	9.0	37.0
235-239	25.194649986001515	27.2	17.8	34.6	8.6	37.0
240-244	24.310975850019183	25.8	16.6	33.8	8.0	37.0
245-249	23.397668474372402	25.0	15.2	32.8	8.0	36.2
250-254	22.464725888903864	23.2	13.0	31.8	8.0	36.0
255-259	21.886676033554888	22.2	12.6	31.0	8.0	36.0
260-264	20.970783604143552	20.6	11.6	29.6	7.8	35.6
265-269	20.24057020500005	19.8	10.4	28.8	7.2	34.8
270-274	19.353711672663547	19.0	9.0	27.2	7.0	33.8
275-279	18.192613465506692	17.4	8.4	25.4	7.0	32.4
280-284	17.921619884071795	17.2	8.2	24.8	7.0	31.6
285-289	16.799609079314386	15.4	8.0	23.2	7.0	30.2
290-294	16.391314717075044	14.4	8.0	22.4	7.0	29.8
295-299	15.350278932796897	13.0	8.0	21.0	7.0	27.6
300-301	13.671183338690778	11.5	8.0	17.5	7.0	24.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.02122405480825762
1101	2	0.06361754799368669
1101	3	0.04645392938846982
1101	4	-0.011954292729846827
1101	5	0.047914710209695954
1101	6	0.15521319694810387
1101	7	0.13273325289672755
1101	8	0.13947232275655352
1101	9	0.17536628000245003
1101	10-14	0.06978570266991113
1101	15-19	0.04161541115300338
1101	20-24	0.0311727661539436
1101	25-29	0.11811439636716159
1101	30-34	0.12559845796421598
1101	35-39	0.1899950885785202
1101	40-44	0.12872229237819255
1101	45-49	0.17755164909667798
1101	50-54	0.12828073401470164
1101	55-59	0.0958065628286846
1101	60-64	0.2552229290747192
1101	65-69	0.21794916377368878
1101	70-74	0.2418400203705673
1101	75-79	0.14907522605609103
1101	80-84	0.24820362140964392
1101	85-89	0.2013214857025858
1101	90-94	0.29928214906552597
1101	95-99	0.07921278838136914
1101	100-104	0.14899972093237324
1101	105-109	0.06335024436598502
1101	110-114	0.15143305537351637
1101	115-119	0.2282255304347771
1101	120-124	0.22491091416182485
1101	125-129	0.20354689055916708
1101	130-134	0.23476543528895633
1101	135-139	0.22527175880983208
1101	140-144	0.17378849969679777
1101	145-149	0.45813681579392806
1101	150-154	0.6251307025720223
1101	155-159	0.2877090062610179
1101	160-164	0.35595335441141884
1101	165-169	0.2791897540214663
1101	170-174	0.2286016659794683
1101	175-179	0.35430311367904466
1101	180-184	0.12192334943461702
1101	185-189	0.1168797359724465
1101	190-194	0.41117582727503077
1101	195-199	0.07696805151025288
1101	200-204	-0.08379082831859108
1101	205-209	-0.020830562797993224
1101	210-214	-0.2800876794343985
1101	215-219	-0.48075589787507056
1101	220-224	-0.039916970563346865
1101	225-229	0.49547132848411124
1101	230-234	0.060748745958044026
1101	235-239	0.3490944416456756
1101	240-244	0.4268131057661968
1101	245-249	0.37302400269429725
1101	250-254	0.13913351462895562
1101	255-259	-0.017596901944415322
1101	260-264	0.6761987848845088
1101	265-269	-0.007333199941101043
1101	270-274	-0.18196389377032673
1101	275-279	0.3255807528409811
1101	280-284	0.04339014291551635
1101	285-289	0.39683423596035183
1101	290-294	0.09168819536276374
1101	295-299	0.06446417740217036
1101	300-301	0.5034336042055596
1102	1	0.037073358984848426
1102	2	9.576535821764764E-5
1102	3	0.04104290722098369
1102	4	0.021506683255374526
1102	5	0.02184866956930165
1102	6	0.07101522896780921
1102	7	0.09587788035977951
1102	8	0.03418830182059196
1102	9	0.10032105986698525
1102	10-14	0.039531507780750985
1102	15-19	0.0717782036406831
1102	20-24	0.09533552015640367
1102	25-29	0.04867108509868956
1102	30-34	0.0710851035922957
1102	35-39	0.02514691277802683
1102	40-44	0.03532597156539197
1102	45-49	0.055973562581904446
1102	50-54	0.021033389494995447
1102	55-59	0.03506841627696389
1102	60-64	0.1837764620180664
1102	65-69	0.21689928692640592
1102	70-74	0.14829091322771148
1102	75-79	-0.01528476470745943
1102	80-84	0.09814993452540932
1102	85-89	0.15770425971736302
1102	90-94	0.14857209672562277
1102	95-99	0.10478044540106879
1102	100-104	0.1001892991343496
1102	105-109	0.010600667703421607
1102	110-114	0.28394075241784833
1102	115-119	0.15990175426482267
1102	120-124	0.18171183011008907
1102	125-129	0.11707925663059626
1102	130-134	0.27252116958698736
1102	135-139	0.10091542414234311
1102	140-144	0.21951009760320517
1102	145-149	0.48500835212397675
1102	150-154	0.802612806943948
1102	155-159	0.4662511860639711
1102	160-164	0.4893354356922046
1102	165-169	0.524066408885993
1102	170-174	0.5419140890090262
1102	175-179	0.5333325163884055
1102	180-184	0.7037973873040784
1102	185-189	0.698539796009392
1102	190-194	0.7944466862651751
1102	195-199	0.7583467845767551
1102	200-204	0.3587872966814025
1102	205-209	0.22860158775619155
1102	210-214	-0.08402163879400248
1102	215-219	-0.15166222484551284
1102	220-224	1.1270715608405908
1102	225-229	1.1989944581023337
1102	230-234	0.8745624389999165
1102	235-239	1.2776956962639048
1102	240-244	1.2201262985001868
1102	245-249	1.5397113872386292
1102	250-254	1.343137132239793
1102	255-259	0.8622435768117427
1102	260-264	1.146556889872194
1102	265-269	1.1109284225958476
1102	270-274	1.2216090742099688
1102	275-279	1.712544461276945
1102	280-284	0.8957304292455674
1102	285-289	1.2828941189652774
1102	290-294	0.9684390805228595
1102	295-299	1.0963947885474905
1102	300-301	0.7472269000799443
1103	1	-0.012273812089809155
1103	2	0.027031534644173405
1103	3	0.05745210900011699
1103	4	0.02949367450316487
1103	5	0.05986100875338707
1103	6	0.06651477461800681
1103	7	0.15079681478993479
1103	8	0.08145502906723578
1103	9	0.1126937223568163
1103	10-14	0.09244460740291771
1103	15-19	0.040440502172415904
1103	20-24	0.08436034200345688
1103	25-29	0.07448006687686615
1103	30-34	0.13552147070693366
1103	35-39	-0.3296677603292508
1103	40-44	-0.17082640301016028
1103	45-49	-0.12943750442273938
1103	50-54	0.1653396690875084
1103	55-59	-0.24688002697713785
1103	60-64	0.22339002555530385
1103	65-69	0.3403217365892246
1103	70-74	0.18970561503076766
1103	75-79	0.30294279280366254
1103	80-84	0.3345495710455637
1103	85-89	0.31651338497442794
1103	90-94	0.4272243517354326
1103	95-99	0.32649358328428235
1103	100-104	0.3248848847673216
1103	105-109	0.23243815395336043
1103	110-114	0.33318366216963113
1103	115-119	0.14437351283768152
1103	120-124	0.5018993091860935
1103	125-129	0.38151753679702693
1103	130-134	0.4526799528860437
1103	135-139	0.44431491718361826
1103	140-144	0.4856930810074829
1103	145-149	0.9690917611822911
1103	150-154	1.0694385757516258
1103	155-159	0.12329456451344356
1103	160-164	-0.28668924267596196
1103	165-169	0.7706138286573889
1103	170-174	0.8439983589406381
1103	175-179	0.9012190724169074
1103	180-184	1.023540081837531
1103	185-189	0.9952713470161356
1103	190-194	0.7905804085857113
1103	195-199	0.7923082365830645
1103	200-204	0.6401465199823768
1103	205-209	0.9411282509155967
1103	210-214	0.915247575419972
1103	215-219	0.6036259285812378
1103	220-224	1.4127734117182058
1103	225-229	2.244807265984111
1103	230-234	1.1398290705939687
1103	235-239	1.1790424847282033
1103	240-244	1.2178727234846392
1103	245-249	1.5922552678642035
1103	250-254	1.2946910085609922
1103	255-259	0.8713104857983041
1103	260-264	1.7660929748116914
1103	265-269	0.9406696823404559
1103	270-274	1.2202842858413234
1103	275-279	1.5990641417972924
1103	280-284	0.6528235470417307
1103	285-289	1.0265748306205467
1103	290-294	1.2270913355326698
1103	295-299	1.5593447144167332
1103	300-301	-0.546048722857547
2116	1	0.059060254212852215
2116	2	0.016543861573339314
2116	3	0.03588231230593664
2116	4	0.04761789417034379
2116	5	0.06990517326151746
2116	6	-0.38037041629565493
2116	7	-0.20141606932016032
2116	8	0.03172430212315902
2116	9	0.03174036529515689
2116	10-14	0.06308322246358244
2116	15-19	0.10799691907047304
2116	20-24	-0.12607329010805302
2116	25-29	-0.15638663830173982
2116	30-34	0.06039894680778701
2116	35-39	-0.19614522152225078
2116	40-44	-0.16360541275616214
2116	45-49	0.06968554125118942
2116	50-54	0.1986378447392667
2116	55-59	0.2350565028478755
2116	60-64	0.33011383898355007
2116	65-69	0.24053193728616407
2116	70-74	-0.7169846557330786
2116	75-79	0.28751167639198627
2116	80-84	-0.17942691181972492
2116	85-89	0.20357001617283288
2116	90-94	0.3401088345261769
2116	95-99	0.3940857790243584
2116	100-104	0.40474363288055315
2116	105-109	0.32158598392932447
2116	110-114	0.47850539222572763
2116	115-119	0.3369561116487816
2116	120-124	-0.013578394259482707
2116	125-129	0.179486714815404
2116	130-134	0.4237825748043065
2116	135-139	0.5590945605132873
2116	140-144	0.6503577475374911
2116	145-149	0.8361126135386456
2116	150-154	1.0678479080662697
2116	155-159	-0.2311399620691148
2116	160-164	-0.603322677258447
2116	165-169	0.8144108582681042
2116	170-174	0.9901907074861853
2116	175-179	0.9408389222203084
2116	180-184	0.997734964717722
2116	185-189	1.3351427668025835
2116	190-194	1.119582887015909
2116	195-199	1.1550881780697573
2116	200-204	0.880476356182367
2116	205-209	1.0481259961031562
2116	210-214	1.1960984410070559
2116	215-219	1.174848558698823
2116	220-224	1.5677733185441411
2116	225-229	1.2834253082105995
2116	230-234	1.3351808026998846
2116	235-239	1.5356006346639113
2116	240-244	2.023717346409189
2116	245-249	1.3448326489275075
2116	250-254	1.3720247394370126
2116	255-259	1.1552666567168117
2116	260-264	0.6874972943614743
2116	265-269	1.3463413741951769
2116	270-274	1.2721118020070286
2116	275-279	1.8726668103025972
2116	280-284	0.5427655927715627
2116	285-289	-0.8537430792987681
2116	290-294	0.5381332079587331
2116	295-299	0.6051367871670443
2116	300-301	1.3638938819166935
1117	1	0.08591661610261525
1117	2	0.07717464581445199
1117	3	0.06490244799767453
1117	4	0.05234962193714665
1117	5	0.06735413846630678
1117	6	-0.5194394107894098
1117	7	-0.30292074747055864
1117	8	-0.0021741941386466124
1117	9	0.12078877479921601
1117	10-14	0.15071681340693743
1117	15-19	0.114881893155939
1117	20-24	0.07182741692525241
1117	25-29	0.12494268205521308
1117	30-34	0.07612700687460006
1117	35-39	-0.1304492138823008
1117	40-44	-0.033405446970483865
1117	45-49	0.13759025248796775
1117	50-54	0.1671785287624985
1117	55-59	0.2944192893223203
1117	60-64	0.31408085788225293
1117	65-69	0.32899531950337035
1117	70-74	-0.05438993188212038
1117	75-79	0.2272909000276826
1117	80-84	0.10288710420837077
1117	85-89	0.12607777549200705
1117	90-94	0.38175988229275504
1117	95-99	0.363101515732005
1117	100-104	0.29025255210279255
1117	105-109	0.2106761273850779
1117	110-114	0.4821856875968251
1117	115-119	0.34681885958511316
1117	120-124	0.45120961794487613
1117	125-129	0.4168134108211632
1117	130-134	0.2823403847869983
1117	135-139	0.49441257510513026
1117	140-144	0.4179306550591875
1117	145-149	0.9691634594031413
1117	150-154	1.0352986215852482
1117	155-159	-0.06285569746081876
1117	160-164	-0.4962886264896582
1117	165-169	0.4232804509484893
1117	170-174	0.658837418305616
1117	175-179	0.7496393342960843
1117	180-184	0.7889824902891718
1117	185-189	1.0315989527329776
1117	190-194	1.2466377671623903
1117	195-199	0.960611346992625
1117	200-204	1.1025679039292093
1117	205-209	1.4532058827823242
1117	210-214	0.8815208844735309
1117	215-219	0.6367204580260086
1117	220-224	1.2143087425855263
1117	225-229	0.7511931621643235
1117	230-234	1.0426034303973175
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2108	150-154	0.30702072661048163
2108	155-159	0.09912727549178868
2108	160-164	-0.05762837635781182
2108	165-169	0.0801302588291577
2108	170-174	-0.3447096320974552
2108	175-179	-0.3229444824748029
2108	180-184	-0.09707604960384231
2108	185-189	-0.5924321630660145
2108	190-194	-0.2095633554172771
2108	195-199	-1.018287357143592
2108	200-204	-0.5017896263955137
2108	205-209	0.021522201625082005
2108	210-214	0.5989387628732921
2108	215-219	-0.41597825364430463
2108	220-224	-0.03793980710180733
2108	225-229	0.4736534198302671
2108	230-234	0.2583839623041939
2108	235-239	-0.15110688046970822
2108	240-244	0.27858594230465883
2108	245-249	0.5611219594250656
2108	250-254	-0.004201821909507686
2108	255-259	-0.13753380098287593
2108	260-264	0.31015010699989176
2108	265-269	-0.2377358441718691
2108	270-274	-0.2371802399241716
2108	275-279	0.28580611341790174
2108	280-284	-0.006183174392177193
2108	285-289	0.5336491398065029
2108	290-294	-0.045195819060314335
2108	295-299	0.4774179033637065
2108	300-301	-0.11539151598674735
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	7.0
14	25.0
15	108.0
16	311.0
17	850.0
18	1624.0
19	1470.0
20	1328.0
21	1918.0
22	2525.0
23	3645.0
24	5369.0
25	7084.0
26	9317.0
27	12728.0
28	17286.0
29	24295.0
30	33908.0
31	44352.0
32	56704.0
33	65571.0
34	59249.0
35	31790.0
36	4281.0
37	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.79470960918301	19.18933211667479	14.863022221300511	34.152936052841696
2	30.32979396302326	21.63803025746845	15.706042161366252	32.32613361814204
3	49.02166136106762	20.864225054179325	17.410228227169505	12.70388535758355
4	49.59585852196726	20.810564190835656	17.017233691763707	12.576343595433384
5	48.20249069359907	20.776345669283174	18.085266334159417	12.935897302958347
6	27.32478561577785	21.99265857173965	37.04025342444447	13.642302388038036
7	16.370970250624747	32.54077707151671	37.555864328746665	13.532388349111873
8	26.819803191654827	37.81224400916642	20.00254046599405	15.365412333184707
9	13.83517041860658	19.187776729331492	40.09244185443649	26.88461099762544
10-14	24.21354431298541	34.69535146569334	26.463256566326905	14.62784765499435
15-19	24.884642105372308	33.58952291085557	20.273592633685542	21.252242350086583
20-24	24.20618214622715	29.205922915003267	24.183214259791164	22.404680678978423
25-29	19.114310600483204	26.474247970219515	32.763041922873526	21.64839950642375
30-34	20.090679082114082	28.580553510509233	30.358827860098092	20.96993954727859
35-39	23.306753491844585	27.924128205394084	28.72489345596698	20.044224846794346
40-44	20.544074492684494	27.025684629662273	30.709567706011054	21.720673171642176
45-49	21.381961654517365	28.319922438017812	28.951357853150693	21.346758054314126
50-54	19.555366604796813	29.633343356940657	30.27556279098705	20.535727247275478
55-59	22.196777237424694	27.36792169143189	25.3799811279669	25.055319943176517
60-64	19.628729041155548	28.541046671989545	28.966289571646325	22.86393471520858
65-69	20.013220792418004	28.15790292309128	27.876533352689265	23.95234293180145
70-74	19.937110505086117	28.35714804176733	26.555542882029055	25.150198571117492
75-79	18.382708240442145	34.01523242671533	26.112153796700504	21.489905536142018
80-84	21.136780762969337	28.82884517674385	25.04775039143915	24.986623668847667
85-89	22.679206545069942	28.457885295368058	25.61302999823723	23.249878161324773
90-94	21.113553645309473	28.649975632264958	27.52631196922407	22.710158753201508
95-99	22.90852248571636	26.906386420431566	26.76743848443057	23.4176526094215
100-104	21.655606134447684	26.294393347089873	29.23350511722436	22.816495401238086
105-109	23.11134499528199	25.49933118344238	30.765613496614442	20.623710324661186
110-114	21.271788384367323	27.86549010255187	29.255332386275263	21.607389126805547
115-119	19.53685749541161	29.671191115627494	27.112630782152447	23.67932060680845
120-124	24.37359367061044	28.493555511774282	27.02869171185931	20.10415910575597
125-129	22.564367112890015	28.641213616897726	27.876066736486276	20.91835253372598
130-134	22.148612076027334	26.386679662792023	29.498491274277004	21.96621698690364
135-139	20.520899221269403	25.206555439189536	31.128070593846886	23.14447474569417
140-144	20.943757193666464	28.132861186864233	27.170128267609577	23.753253351859723
145-149	20.677785957963067	29.200271674322632	28.206119930733415	21.91582243698089
150-154	20.796876782214664	26.449361772726803	28.778087703107662	23.97567374195087
155-159	22.592519623803646	29.84710542415413	27.847810533083088	19.712564418959136
160-164	20.70930847478717	25.965273385248704	29.445297027136323	23.880121112827798
165-169	23.565362560789723	27.255259801532578	26.190493472557787	22.98888416511992
170-174	18.23650183017244	28.5036655295057	31.673959705098557	21.585872935223303
175-179	19.88904903617831	26.867398044359646	30.54355603023673	22.699996889225314
180-184	23.03440516803368	27.786269040533394	25.410363027405925	23.768962764027002
185-189	21.25540497101795	29.58704466035525	26.954551581828927	22.202998786797874
190-194	20.084250147761797	28.018488370887297	27.285278777258164	24.611982704092743
195-199	21.97923039434254	28.378145770901813	29.259739317081262	20.382884517674384
200-204	21.76728294569624	27.581009757463264	27.881095822229597	22.770611474610895
205-209	22.701189352855174	27.072605481184997	27.896597849417766	22.329607316542063
210-214	20.864795362871867	28.64002115326787	27.46088200831614	23.034301475544126
215-219	22.331370088864464	29.321488194610062	25.833324692292535	22.513817024232935
220-224	20.9597776833024	28.100612822613257	28.825060400875163	22.114549093209177
225-229	21.354586837275377	28.88110619147855	25.93846887669926	23.82583809454681
230-234	19.911705845145637	27.989869243770677	26.176546832712905	25.921878078370785
235-239	20.776812285486162	29.228476031481037	25.417725194164188	24.57698648886861
240-244	19.71235703398003	27.43972874044733	27.608280882215702	25.239633343356942
245-249	21.613662522423503	27.329348085318177	26.803056854592022	24.253932537666298
250-254	21.716629164549612	27.550576011779466	25.55745082383683	25.175343999834094
255-259	20.55314758552038	30.22267962131503	24.34948516678937	24.874687626375223
260-264	20.752963012888976	31.11059840935721	24.194724126131543	23.94171445162227
265-269	21.235962629226766	28.946847229855145	24.13567125333112	25.681518887586975
270-274	19.301682929105446	28.6150312632856	25.957081678574024	26.126204129034935
275-279	20.28987235454536	30.12214975269341	25.168811372992256	24.41916651976897
280-284	19.171548854716452	28.408683209075164	25.713870944327503	26.705896991880877
285-289	20.621221704911914	29.9920675245492	23.74946857599104	25.63724219454785
290-294	19.463443212808095	30.3649975632265	23.092576654672904	27.078982569292503
295-299	20.019857111749396	30.536142017233693	23.36196974253155	26.08203112848536
300-301	17.679828700007256	29.386970001762773	29.954038304005643	22.97916299422433
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.25
18	0.0
19	0.25
20	0.25
21	0.25
22	0.25
23	0.25
24	0.0
25	1.5
26	2.25
27	1.25
28	1.0
29	3.75
30	10.75
31	47.5
32	232.75
33	668.0
34	1626.0
35	3378.75
36	5695.75
37	6023.0
38	6682.75
39	7367.5
40	9264.0
41	10520.5
42	9160.25
43	8289.25
44	7852.75
45	6950.5
46	4862.5
47	3174.75
48	2213.75
49	2189.75
50	2710.75
51	1995.75
52	2701.75
53	4968.0
54	4357.25
55	3501.0
56	3614.0
57	3083.0
58	1363.5
59	884.0
60	876.5
61	712.75
62	363.25
63	199.75
64	141.75
65	154.25
66	221.75
67	171.75
68	89.25
69	46.25
70	31.25
71	25.0
72	18.25
73	15.5
74	12.5
75	11.0
76	9.75
77	6.25
78	5.25
79	3.25
80	0.75
81	1.25
82	0.75
83	0.25
84	0.25
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.25
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	385756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.60745810188691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	45.814850484499374	6.234236584120384
2	9.30856445325217	2.5333180157268544
3	6.208626903424849	2.534508913758045
4	4.907651815658923	2.6712266584091213
5	3.996398624485852	2.7190413420564856
6	3.601417681003652	2.9403684120991147
7	3.452278982800843	3.2883719130141063
8	3.0139644647593493	3.2809916139831077
9	2.6920098745132672	3.296827041957461
>10	16.176137772958178	36.84171043596931
>50	0.4933981137881229	4.532378830800692
>100	0.24004387295437518	6.553594182617905
>500	0.04732847795230619	4.838137957122219
>1k	0.04164906059480426	10.360998276098094
>5k	0.001893139117945648	1.4274065501705506
>10k+	0.003786278235891296	5.946883272096582
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGGGTTATTAGTAGAAACACGAGCAATTAGTATGGGTTAGCTTCACGCG	12668	3.2839411441429296	No Hit
GGGGGGAGCAGAAGCAGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAAT	10417	2.7004116591835254	No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT	5541	1.4364002115326786	No Hit
CCGGGTTATTAGTAGAAACACGTGCAATTAGTATGGGTTAGCTTCACGCG	3381	0.8764607679465776	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA	3193	0.8277252978566763	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT	3104	0.8046537189311378	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG	2853	0.7395866817366419	No Hit
CCGGGTTATTAGTAGAAACACGAGCGTGCCAAACAATTCCTGCAACAGGT	2788	0.7227366521842823	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT	2676	0.6937027551094475	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA	2298	0.5957133524818797	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA	2274	0.5894918031087009	No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAACATAAATCCGTATTTTCT	2102	0.5449040326009187	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT	1805	0.46791235910782986	No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	1262	0.3271498045396572	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG	1257	0.32585364842024495	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT	1217	0.3154843994649467	No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAACATAAATCCGTATTTTCT	1187	0.3077074627484731	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1170	0.3033005319424714	No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT	1156	0.29967129480811705	No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	1153	0.2988936011364697	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG	1122	0.2908574331961136	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA	1100	0.28515434627069963	No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT	1082	0.28048818424081545	No Hit
GGGGGGAGCAGAAGCGGTGCGCAGTTGCTCGACGGTCAGGTCGGGCATAT	1026	0.265971235703398	No Hit
CCGGGTTATTAGTAGAAACACGAGCCAACCTACCCGAACAGCGAGCCACA	1014	0.26286046101680854	No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAACATAAATCCGTATTTTCT	987	0.2558612179719823	No Hit
GGGGGGAGCAGAAGCGGTGCGCAGTTGCTCGACGGTCAGGTCAGGCATAT	973	0.25223198083762793	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCTGATACCTCCAGCACACTTTA	942	0.24419581289727185	No Hit
GGGGGGAGCAGAAGCGGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAAT	903	0.23408579516585615	No Hit
CCGGGTTATTAGTAGAAACACGAGCGGTGTCCCTGGACCAGATGCTCGCC	885	0.22941963313597197	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT	874	0.22656808967326494	TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGAGCAGAAGCAGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAATA	830	0.215161915822437	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA	803	0.20816267277761072	No Hit
CCGGGTTATTAGTAGAAACACGTGCGTGCCAAACAATTCCTGCAACAGGT	803	0.20816267277761072	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT	785	0.20349651074772654	No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	760	0.19701573015066517	No Hit
GGGGGGAGCAGAAGCGGAGCGCAGTTGCTCGACGGTCAGGTCAGGCATAT	752	0.19494188035960555	No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	750	0.19442341791184065	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA	716	0.1856095562998372	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG	696	0.1804249318221881	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA	685	0.17757338835948114	No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	682	0.17679569468783377	No Hit
CCGGGTTATTAGTAGTAACAAGAGCAATTAGTATGGGTTAGCTTCACGCG	665	0.17238876388183205	No Hit
GGGGGGAGCAGAAGCGGTGCGAATGGATGGGGGGTTGGGGTGATGTTGAG	662	0.17161107021018468	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG	641	0.16616721450865315	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT	607	0.1573533528966497	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	605	0.1568348904488848	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTGACAAGA	598	0.1550202718817076	No Hit
CCGGGTTATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGCA	592	0.1534648845384129	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA	585	0.1516502659712357	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC	489	0.1267640684785201	No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCAATTATTAAAGCAACA	485	0.12572714358299028	No Hit
CCGGGTTATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTA	443	0.1148394321799272	No Hit
CCGGGTTATTAGTAGAAACAACAGCAATTAGTATGGGTTAGCTTCACGCG	431	0.11172865749333775	No Hit
GGGGGGAGCAGAAGCGGAGCGCAGTTGCTCGACGGTCAGGTCGGGCATAT	421	0.10913634525451321	No Hit
CCGGGTTATTAGTAGTAACAAGAGCGTGCCAAACAATTCCTGCAACAGGT	420	0.10887711403063077	No Hit
GGGGGGAGCAGAAGCGGAGCGAATGGATGGGGGGTTGGGGTGATGTTGAG	411	0.10654403301568868	No Hit
CCGGGTTATTAGTAGAAACAACAGCGTGCCAAACAATTCCTGCAACAGGT	407	0.10550710812015886	No Hit
GGGGGGAGCAGAAGCGGAGCATCACCAGCAGACCGGCCGCTGCGCAATAC	402	0.10421095200074659	No Hit
GGGGGGAGCAGAAGCGGTGCTAACGTCCGTTGTCAAGAGGGAAACAACCC	395	0.10239633343356941	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.592312238824542E-4	0.0	0.0
2	2.592312238824542E-4	0.0	0.0
3	5.184624477649084E-4	0.0	0.0
4	5.184624477649084E-4	0.0	0.0
5	5.184624477649084E-4	0.0	0.0
6	0.0010369248955298168	0.0	0.0
7	0.001296156119412271	0.0	0.0
8	0.001296156119412271	0.0	0.0
9	0.0015553873432947252	0.0	0.0
10-14	0.0019183110567301612	0.0	0.0
15-19	0.0024367735044950696	0.0	0.0
20-24	0.0037329296239073406	0.0	0.0
25-29	0.004562469540331193	0.0	0.0
30-34	0.007776936716473626	0.0	0.0
35-39	0.01109509638216904	0.0	0.0
40-44	0.05822333288399921	0.0	0.0
45-49	0.12629745227553166	0.0	0.0
50-54	0.1324153091591576	0.0	0.0
55-59	0.1410217857920551	0.0	0.0
60-64	0.15305011458020096	0.0	0.0
65-69	0.17461815240722114	0.0	0.0
70-74	0.2025632783417497	0.0	0.0
75-79	0.23802610976886945	0.0	0.0
80-84	0.2908574331961136	0.0	0.0
85-89	0.38060328290421924	0.0	0.0
90-94	0.4430261616151142	0.0	0.0
95-99	0.520432605066415	0.0	0.0
100-104	0.606186293926731	0.0	0.0
105-109	0.707804933688653	0.0	0.0
110-114	0.8241997532118749	0.0	0.0
115-119	0.9576001410217858	0.0	0.0
120-124	1.0831198996256703	0.0	0.0
125-129	1.2100395068385197	0.0	0.0
130-134	1.352564833729093	0.0	0.0
135-139	1.5083109530376715	0.0	0.0
140-144	1.7273613372183452	0.0	0.0
145-149	1.9911550306411308	0.0	0.0
150-154	2.270761828720746	0.0	0.0
155-159	2.8644531776563427	0.0	0.0
160-164	3.2110453239871832	0.0	0.0
165-169	3.8869648171382947	0.0	0.0
170-174	4.538257344020573	0.0	0.0
175-179	4.925548792500959	0.0	0.0
180-184	5.3091073113574385	0.0	0.0
185-189	5.76576903534877	0.0	0.0
190-194	6.251620195149266	0.0	0.0
195-199	6.811093022532378	0.0	0.0
200-204	7.421219631062122	0.0	0.0
205-209	8.108700836798391	0.0	0.0
210-214	8.881987577639752	0.0	0.0
215-219	9.70198778502473	0.0	0.0
220-224	10.600794284469977	0.0	0.0
225-229	11.558031501778325	0.0	0.0
230-234	12.561930339385517	0.0	0.0
235-239	13.501125063511651	0.0	0.0
240-244	14.473553230539512	0.0	0.0
245-249	15.50026441584836	0.0	0.0
250-254	16.643525959414763	0.0	0.0
255-259	18.59921815862877	0.0	0.0
260-264	19.786600856499962	0.0	0.0
265-269	20.702257385497568	0.0	0.0
270-274	21.61024067026825	0.0	0.0
275-279	22.510084094609027	0.0	0.0
280-284	23.367413598233078	0.0	0.0
285-289	24.178599944006056	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTT	7495	0.0	286.7345	1
CGGGTTA	7540	0.0	284.82758	2
GTTATTA	8010	0.0	267.74655	5
TTATTAG	8035	0.0	266.3628	6
GGGGGGA	5140	0.0	266.0165	1
TATTAGT	8195	0.0	261.3423	7
GGGTTAT	8290	0.0	259.41495	3
TTAGTAG	8645	0.0	247.73859	9
GGTTATT	8825	0.0	243.35411	4
GGGAGCA	5765	0.0	234.87425	4
GGGGAGC	5805	0.0	233.5099	3
GGGGGAG	5845	0.0	232.66893	2
GAGCAGA	5905	0.0	229.05588	6
CAGAAGC	5925	0.0	227.28691	9
ATTAGTA	9955	0.0	215.13812	8
GCAGAAG	6500	0.0	207.40768	8
AGCAGAA	6940	0.0	194.25792	7
GGAGCAG	7045	0.0	192.61887	5
GCTAGGG	145	0.0	172.93103	295
GATCGGG	150	0.0	147.5	295
>>END_MODULE