FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_30515_flu.3510000003ba93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_30515_flu.3510000003ba93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385756
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACACGAGCAATTAGTATGGGTTAGCTTCACGCG126683.2839411441429296No Hit
GGGGGGAGCAGAAGCAGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAAT104172.7004116591835254No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT55411.4364002115326786No Hit
CCGGGTTATTAGTAGAAACACGTGCAATTAGTATGGGTTAGCTTCACGCG33810.8764607679465776No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA31930.8277252978566763No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT31040.8046537189311378No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG28530.7395866817366419No Hit
CCGGGTTATTAGTAGAAACACGAGCGTGCCAAACAATTCCTGCAACAGGT27880.7227366521842823No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT26760.6937027551094475No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA22980.5957133524818797No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA22740.5894918031087009No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAACATAAATCCGTATTTTCT21020.5449040326009187No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT18050.46791235910782986No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC12620.3271498045396572No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG12570.32585364842024495No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT12170.3154843994649467No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAACATAAATCCGTATTTTCT11870.3077074627484731No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA11700.3033005319424714No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT11560.29967129480811705No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT11530.2988936011364697No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG11220.2908574331961136No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA11000.28515434627069963No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT10820.28048818424081545No Hit
GGGGGGAGCAGAAGCGGTGCGCAGTTGCTCGACGGTCAGGTCGGGCATAT10260.265971235703398No Hit
CCGGGTTATTAGTAGAAACACGAGCCAACCTACCCGAACAGCGAGCCACA10140.26286046101680854No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAACATAAATCCGTATTTTCT9870.2558612179719823No Hit
GGGGGGAGCAGAAGCGGTGCGCAGTTGCTCGACGGTCAGGTCAGGCATAT9730.25223198083762793No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCTGATACCTCCAGCACACTTTA9420.24419581289727185No Hit
GGGGGGAGCAGAAGCGGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAAT9030.23408579516585615No Hit
CCGGGTTATTAGTAGAAACACGAGCGGTGTCCCTGGACCAGATGCTCGCC8850.22941963313597197No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT8740.22656808967326494TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGAGCAGAAGCAGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAATA8300.215161915822437No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA8030.20816267277761072No Hit
CCGGGTTATTAGTAGAAACACGTGCGTGCCAAACAATTCCTGCAACAGGT8030.20816267277761072No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT7850.20349651074772654No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC7600.19701573015066517No Hit
GGGGGGAGCAGAAGCGGAGCGCAGTTGCTCGACGGTCAGGTCAGGCATAT7520.19494188035960555No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA7500.19442341791184065No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA7160.1856095562998372No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG6960.1804249318221881No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA6850.17757338835948114No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC6820.17679569468783377No Hit
CCGGGTTATTAGTAGTAACAAGAGCAATTAGTATGGGTTAGCTTCACGCG6650.17238876388183205No Hit
GGGGGGAGCAGAAGCGGTGCGAATGGATGGGGGGTTGGGGTGATGTTGAG6620.17161107021018468No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG6410.16616721450865315No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT6070.1573533528966497No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT6050.1568348904488848No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTGACAAGA5980.1550202718817076No Hit
CCGGGTTATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGCA5920.1534648845384129No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA5850.1516502659712357No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC4890.1267640684785201No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCAATTATTAAAGCAACA4850.12572714358299028No Hit
CCGGGTTATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTA4430.1148394321799272No Hit
CCGGGTTATTAGTAGAAACAACAGCAATTAGTATGGGTTAGCTTCACGCG4310.11172865749333775No Hit
GGGGGGAGCAGAAGCGGAGCGCAGTTGCTCGACGGTCAGGTCGGGCATAT4210.10913634525451321No Hit
CCGGGTTATTAGTAGTAACAAGAGCGTGCCAAACAATTCCTGCAACAGGT4200.10887711403063077No Hit
GGGGGGAGCAGAAGCGGAGCGAATGGATGGGGGGTTGGGGTGATGTTGAG4110.10654403301568868No Hit
CCGGGTTATTAGTAGAAACAACAGCGTGCCAAACAATTCCTGCAACAGGT4070.10550710812015886No Hit
GGGGGGAGCAGAAGCGGAGCATCACCAGCAGACCGGCCGCTGCGCAATAC4020.10421095200074659No Hit
GGGGGGAGCAGAAGCGGTGCTAACGTCCGTTGTCAAGAGGGAAACAACCC3950.10239633343356941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT74950.0286.73451
CGGGTTA75400.0284.827582
GTTATTA80100.0267.746555
TTATTAG80350.0266.36286
GGGGGGA51400.0266.01651
TATTAGT81950.0261.34237
GGGTTAT82900.0259.414953
TTAGTAG86450.0247.738599
GGTTATT88250.0243.354114
GGGAGCA57650.0234.874254
GGGGAGC58050.0233.50993
GGGGGAG58450.0232.668932
GAGCAGA59050.0229.055886
CAGAAGC59250.0227.286919
ATTAGTA99550.0215.138128
GCAGAAG65000.0207.407688
AGCAGAA69400.0194.257927
GGAGCAG70450.0192.618875
GCTAGGG1450.0172.93103295
GATCGGG1500.0147.5295
Produced by FastQC (version 0.11.2)