##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AJTU1_l01n01_30455_flu.3510000003bb35.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 401532 Sequences flagged as poor quality 0 Sequence length 301 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.77411762947909 34.0 34.0 34.0 34.0 34.0 2 33.86277058864549 34.0 34.0 34.0 34.0 34.0 3 33.887453552892424 34.0 34.0 34.0 34.0 34.0 4 33.89626231533228 34.0 34.0 34.0 34.0 34.0 5 33.879020850143945 34.0 34.0 34.0 34.0 34.0 6 37.61037227418985 38.0 38.0 38.0 38.0 38.0 7 37.63199446121355 38.0 38.0 38.0 38.0 38.0 8 37.63592191905004 38.0 38.0 38.0 38.0 38.0 9 37.5523495014096 38.0 38.0 38.0 37.0 38.0 10-14 37.64698355299204 38.0 38.0 38.0 37.8 38.0 15-19 37.60713068945937 38.0 38.0 38.0 37.2 38.0 20-24 37.59521980813484 38.0 38.0 38.0 37.0 38.0 25-29 37.485357082374506 38.0 38.0 38.0 37.0 38.0 30-34 37.40310062460775 38.0 38.0 38.0 37.0 38.0 35-39 37.32955480509648 38.0 38.0 38.0 37.0 38.0 40-44 37.31973989619756 38.0 38.0 38.0 37.0 38.0 45-49 37.287952641383505 38.0 38.0 38.0 37.0 38.0 50-54 37.250209198768715 38.0 38.0 38.0 37.0 38.0 55-59 37.17650398971938 38.0 38.0 38.0 37.0 38.0 60-64 37.028530229222085 38.0 38.0 38.0 36.4 38.0 65-69 36.93456660988414 38.0 38.0 38.0 35.8 38.0 70-74 37.02642379685803 38.0 38.0 38.0 36.0 38.0 75-79 36.93533118157457 38.0 38.0 38.0 36.0 38.0 80-84 36.802440652301684 38.0 38.0 38.0 35.6 38.0 85-89 36.86376179233535 38.0 38.0 38.0 36.0 38.0 90-94 36.69637488419354 38.0 38.0 38.0 35.2 38.0 95-99 36.70300150423877 38.0 38.0 38.0 35.0 38.0 100-104 36.63609226661885 38.0 38.0 38.0 34.8 38.0 105-109 36.47888287857506 38.0 38.0 38.0 34.0 38.0 110-114 36.40357231802198 38.0 37.0 38.0 34.0 38.0 115-119 36.46137094926432 38.0 37.0 38.0 34.0 38.0 120-124 36.335044280406045 38.0 37.0 38.0 33.8 38.0 125-129 36.23277098711934 38.0 37.0 38.0 34.0 38.0 130-134 36.099697159877664 38.0 37.0 38.0 33.2 38.0 135-139 35.812481695107735 38.0 37.0 38.0 31.6 38.0 140-144 35.75877588834763 38.0 37.0 38.0 31.0 38.0 145-149 35.264274827411015 38.0 36.2 38.0 27.6 38.0 150-154 34.84424354721417 38.0 35.2 38.0 26.6 38.0 155-159 34.959600231114834 38.0 35.6 38.0 27.0 38.0 160-164 34.83682197184782 38.0 35.2 38.0 25.6 38.0 165-169 34.476869589472315 38.0 35.0 38.0 24.2 38.0 170-174 34.437031170616535 38.0 35.0 38.0 23.8 38.0 175-179 34.040136278055044 37.8 34.2 38.0 22.8 38.0 180-184 33.73033880238686 37.0 34.0 38.0 22.2 38.0 185-189 32.83470856619149 37.0 31.8 37.8 20.6 38.0 190-194 31.77124762160924 36.2 28.8 37.0 18.6 38.0 195-199 31.449070559756137 35.8 28.0 37.2 18.0 37.8 200-204 31.321327316378273 36.4 28.0 37.0 17.8 37.4 205-209 30.070998575456002 34.4 25.0 37.0 15.2 37.2 210-214 29.16396949682715 33.0 23.4 37.0 12.8 37.0 215-219 28.314454140641345 32.0 22.0 36.6 10.4 37.0 220-224 26.726265403504577 29.6 20.0 36.0 9.0 37.0 225-229 25.740396282239026 28.2 18.6 35.2 9.0 37.0 230-234 25.32905870515924 27.6 18.0 34.6 8.6 37.0 235-239 24.230632676847677 25.8 16.4 33.4 8.0 37.0 240-244 23.24532042278075 24.4 14.6 32.4 8.0 36.6 245-249 22.298101770220057 23.0 13.0 31.0 8.0 36.0 250-254 21.38804976938326 21.0 12.2 30.2 7.0 36.0 255-259 20.829482581712043 20.4 11.4 29.6 7.2 35.8 260-264 19.622065987268762 18.8 10.2 27.8 7.0 34.2 265-269 18.774499168185848 18.0 8.6 26.6 7.0 32.8 270-274 17.960553579789405 17.2 8.0 25.2 7.0 32.0 275-279 16.83344839265613 15.0 8.0 23.4 7.0 30.2 280-284 16.534828606437344 14.6 8.0 22.8 7.0 30.0 285-289 15.487293665262047 13.0 8.0 21.2 7.0 27.8 290-294 14.951551557534643 12.8 8.0 20.4 7.0 26.8 295-299 14.220124921550461 12.0 8.0 19.0 7.0 26.0 300-301 12.607033810505762 10.0 7.5 16.0 7.0 22.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.05629756675996589 1101 2 0.04746271567636029 1101 3 0.03418476795400949 1101 4 0.012768898131341189 1101 5 -0.0023097626593013842 1101 6 0.09274982782632435 1101 7 -0.0096758644902053 1101 8 -0.06371368468989402 1101 9 -0.07558866340885118 1101 10-14 0.029427597990206777 1101 15-19 0.0399539326317111 1101 20-24 0.048535207802437697 1101 25-29 0.07015232522201842 1101 30-34 0.004398328638679061 1101 35-39 0.12353535475052269 1101 40-44 0.08605890091529034 1101 45-49 0.07071519543685412 1101 50-54 0.07832175136537245 1101 55-59 0.14916308856319915 1101 60-64 0.25731500874213964 1101 65-69 0.22435065461865378 1101 70-74 0.12823702144184068 1101 75-79 0.10110448176057218 1101 80-84 0.17463151553415912 1101 85-89 0.11161653266257332 1101 90-94 0.2683315677547782 1101 95-99 0.1412586275289911 1101 100-104 0.15566892712107716 1101 105-109 0.11061662512230441 1101 110-114 0.25430259370182284 1101 115-119 0.12104029191914378 1101 120-124 0.21468093845496838 1101 125-129 0.12642942733062768 1101 130-134 0.13154125867339417 1101 135-139 0.14581693579744837 1101 140-144 0.25791055787993145 1101 145-149 0.32785315476796484 1101 150-154 0.648324184733788 1101 155-159 0.41598830339088977 1101 160-164 0.26780109388199946 1101 165-169 0.0467157440250503 1101 170-174 0.23573684290598607 1101 175-179 0.22959581718545508 1101 180-184 -0.03596160060691744 1101 185-189 0.13696249862589127 1101 190-194 0.4063030797765528 1101 195-199 4.8783512112748895E-4 1101 200-204 -0.22374742427124517 1101 205-209 -0.30514860576834124 1101 210-214 -0.583448435489732 1101 215-219 -0.6187613804989311 1101 220-224 0.35297255197284017 1101 225-229 0.6769997029622274 1101 230-234 0.38991898814516546 1101 235-239 0.4454289120529147 1101 240-244 0.4865254813724249 1101 245-249 0.42514012218336816 1101 250-254 0.03198658332479454 1101 255-259 0.44422821614162444 1101 260-264 0.738845573096711 1101 265-269 0.47620127449617655 1101 270-274 0.23028518214813687 1101 275-279 0.295552127298194 1101 280-284 0.09792974726193293 1101 285-289 0.30875825196132745 1101 290-294 0.14663350793062513 1101 295-299 0.5840348487271569 1101 300-301 0.11225600526059765 1102 1 -0.12326948528979642 1102 2 -0.06016408475672819 1102 3 0.012019071604271403 1102 4 0.0325841429829552 1102 5 0.058650687052519856 1102 6 0.01545287439059706 1102 7 0.14753188297706288 1102 8 0.04836703449905144 1102 9 0.11422647207704273 1102 10-14 0.050837759523375325 1102 15-19 0.09304624823199248 1102 20-24 0.06434712064525883 1102 25-29 0.05037754568506614 1102 30-34 0.08317281288819345 1102 35-39 0.08835732559361986 1102 40-44 0.08126503706101573 1102 45-49 0.1132404133480378 1102 50-54 0.19677320574710677 1102 55-59 0.11884709455644327 1102 60-64 0.26427814155951523 1102 65-69 0.30235028277522247 1102 70-74 0.17370745806623233 1102 75-79 0.006216053017809031 1102 80-84 0.18840995517703618 1102 85-89 0.20095720322022714 1102 90-94 0.3031605380036595 1102 95-99 0.2217568977279214 1102 100-104 0.23513824821516494 1102 105-109 0.16026756621428717 1102 110-114 0.3549968837178312 1102 115-119 0.16046444497280987 1102 120-124 0.2654401187953894 1102 125-129 0.0746564268093195 1102 130-134 0.23175397498967243 1102 135-139 0.1436325379103991 1102 140-144 0.346914928863022 1102 145-149 0.4084367302248495 1102 150-154 0.994588434175121 1102 155-159 0.6625026357678792 1102 160-164 0.703605788587609 1102 165-169 0.6182482128831666 1102 170-174 0.599701386541085 1102 175-179 0.7503404883409743 1102 180-184 0.7099780149361337 1102 185-189 0.8309052784836481 1102 190-194 1.2088059141713394 1102 195-199 0.9928203870971828 1102 200-204 0.48878625125525943 1102 205-209 0.32434349984977473 1102 210-214 0.015476681804994286 1102 215-219 0.016600565335881612 1102 220-224 1.0078079747989221 1102 225-229 1.38560222785204 1102 230-234 0.9804502867901235 1102 235-239 1.1051374888768635 1102 240-244 1.3471038801854434 1102 245-249 1.5890833395039792 1102 250-254 1.0302956070899647 1102 255-259 0.8106709868855653 1102 260-264 1.0518340277226947 1102 265-269 1.320151892230193 1102 270-274 1.347954671665434 1102 275-279 1.805414362952142 1102 280-284 0.7045150215329166 1102 285-289 1.4021979495286718 1102 290-294 1.0594212764690205 1102 295-299 0.8636499907749933 1102 300-301 0.5339715705537529 1103 1 -0.07893777948822844 1103 2 -0.008309016283888582 1103 3 -0.003349806175506842 1103 4 0.013878118997162403 1103 5 0.05272308220735056 1103 6 0.12166167859605537 1103 7 0.08854003475764216 1103 8 0.08091678719188877 1103 9 0.13768976082095463 1103 10-14 0.08406482668289073 1103 15-19 0.026852307169122014 1103 20-24 0.04075232309821075 1103 25-29 0.083800905178947 1103 30-34 0.0744701075138039 1103 35-39 -0.5215330190124021 1103 40-44 -0.2262558543038793 1103 45-49 -0.07926499704769441 1103 50-54 0.09067825815645847 1103 55-59 -0.24891206604669236 1103 60-64 0.2846602408283516 1103 65-69 0.3512969766968652 1103 70-74 0.09355081254688713 1103 75-79 0.33337835782872816 1103 80-84 0.2787713307767632 1103 85-89 0.3505247246058545 1103 90-94 0.4258640765613322 1103 95-99 0.38174785980749704 1103 100-104 0.2358978218795187 1103 105-109 0.1632850312894334 1103 110-114 0.33069105602437077 1103 115-119 0.1567694889091129 1103 120-124 0.4073513565315281 1103 125-129 0.3671581302454925 1103 130-134 0.48695325054386274 1103 135-139 0.4939515129537284 1103 140-144 0.37524351375769527 1103 145-149 0.7870415718670642 1103 150-154 1.0851321568804195 1103 155-159 0.05801085992242605 1103 160-164 -0.5607698005541764 1103 165-169 0.578663886614244 1103 170-174 0.9413268353479651 1103 175-179 0.7628048750509961 1103 180-184 0.90099993542362 1103 185-189 0.7409184823205308 1103 190-194 0.3369253206341618 1103 195-199 0.364724987610515 1103 200-204 0.37172399904785536 1103 205-209 0.8736271740468666 1103 210-214 0.8017113288141822 1103 215-219 0.5684485729333808 1103 220-224 1.2642026565476208 1103 225-229 1.952239062320409 1103 230-234 1.1610655361625888 1103 235-239 1.0793561147599107 1103 240-244 1.1274795893621103 1103 245-249 1.2155167854582736 1103 250-254 1.270975251645499 1103 255-259 0.5009624598194655 1103 260-264 1.5684103151850515 1103 265-269 0.6366289743940676 1103 270-274 0.6494745250816649 1103 275-279 1.3166139703674702 1103 280-284 0.6716367616150372 1103 285-289 0.9463886143453895 1103 290-294 0.9042723096516045 1103 295-299 0.9205534085917009 1103 300-301 -0.7935087384301518 2116 1 -0.005087506334987779 2116 2 0.006310404588042218 2116 3 0.039230142432813864 2116 4 0.01835653665171577 2116 5 -0.016245413398443986 2116 6 -0.30209509625225195 2116 7 -0.11104624064129354 2116 8 0.08874991539037325 2116 9 0.1389784395365936 2116 10-14 0.10849237089484376 2116 15-19 0.10987638352247586 2116 20-24 -0.15323303675150157 2116 25-29 -0.2829534296443157 2116 30-34 0.10398119559222607 2116 35-39 -0.18869546421980488 2116 40-44 -0.1535256606133899 2116 45-49 0.09582826373269171 2116 50-54 0.2258307705185203 2116 55-59 0.16285222321503312 2116 60-64 0.3609436292242023 2116 65-69 0.23776455459412915 2116 70-74 -0.7916268774187856 2116 75-79 0.17553093497023298 2116 80-84 -0.13500803049809207 2116 85-89 0.18720974544324065 2116 90-94 0.29843828139387796 2116 95-99 0.26895824179017325 2116 100-104 0.2488839374892038 2116 105-109 0.3685855430468976 2116 110-114 0.37301810575337413 2116 115-119 0.3255648075159172 2116 120-124 -0.031628410694402476 2116 125-129 -0.04957949148584362 2116 130-134 0.280622486794428 2116 135-139 0.6048623548703347 2116 140-144 0.4997754824044591 2116 145-149 0.7687798197995193 2116 150-154 0.9355841013428545 2116 155-159 -0.37007283509603184 2116 160-164 -0.6603922660591337 2116 165-169 0.5555600883805454 2116 170-174 0.7950652560255591 2116 175-179 0.3829341747854116 2116 180-184 0.5539000239554994 2116 185-189 1.0394834021020074 2116 190-194 0.6615852934268069 2116 195-199 0.7608891818624173 2116 200-204 0.5737444261602072 2116 205-209 0.7206934865860006 2116 210-214 0.7147443447283308 2116 215-219 1.0997941279850991 2116 220-224 1.2192443529048589 2116 225-229 0.9447821730284467 2116 230-234 0.7341924390582264 2116 235-239 0.9005224035465922 2116 240-244 1.3958289663999643 2116 245-249 0.7014175865638457 2116 250-254 1.1626816712866237 2116 255-259 0.7441485711105216 2116 260-264 0.06798700322893225 2116 265-269 0.7046822662685734 2116 270-274 0.7074764684644457 2116 275-279 1.384730659706939 2116 280-284 0.3537940028752118 2116 285-289 -0.7868123962136586 2116 290-294 0.34062704178867875 2116 295-299 0.47970039310941637 2116 300-301 1.2647245106899287 1117 1 -0.10152651385638478 1117 2 0.007358213008551218 1117 3 0.054165735067030596 1117 4 0.04353160365266717 1117 5 -0.029126886554841747 1117 6 -0.5031238850479625 1117 7 -0.3652495777209168 1117 8 -0.110802258898552 1117 9 -0.041034528389403135 1117 10-14 0.08269847446534584 1117 15-19 0.04769379512444516 1117 20-24 -0.021298913815570586 1117 25-29 0.0928804079972565 1117 30-34 0.018394094355961954 1117 35-39 -0.16725775349100758 1117 40-44 -0.13100262093153958 1117 45-49 0.0318991550614669 1117 50-54 0.06553371856036705 1117 55-59 0.21291256729733732 1117 60-64 0.1823389780614164 1117 65-69 0.23463928442900084 1117 70-74 -0.19760439968039378 1117 75-79 0.2113123821907763 1117 80-84 0.005252365455916674 1117 85-89 0.05556022525649951 1117 90-94 0.2531025850162507 1117 95-99 0.2556099232979179 1117 100-104 0.18857443632208515 1117 105-109 0.19623022187460037 1117 110-114 0.11986465196241625 1117 115-119 0.14359800540646717 1117 120-124 0.35470114753159265 1117 125-129 0.22069300786575496 1117 130-134 0.09921831976753026 1117 135-139 0.2767717522940387 1117 140-144 0.30659661147853967 1117 145-149 0.621134795160458 1117 150-154 0.8153532722601256 1117 155-159 -0.27153838363930305 1117 160-164 -0.8893451492613522 1117 165-169 0.11182532569360148 1117 170-174 0.3353643828518855 1117 175-179 0.4239754127900639 1117 180-184 0.36255550938820136 1117 185-189 0.8444302336054221 1117 190-194 0.9829334390133901 1117 195-199 0.6281434396647825 1117 200-204 0.5801682179817611 1117 205-209 1.0202797232938785 1117 210-214 0.6890303307229715 1117 215-219 0.2819227698109792 1117 220-224 0.8166514593283054 1117 225-229 0.41793787459324605 1117 230-234 0.9404853412800698 1117 235-239 0.6706915053015834 1117 240-244 0.554187572780183 1117 245-249 1.1659551503228158 1117 250-254 1.0378825012338844 1117 255-259 1.0690967858593758 1117 260-264 0.09129365145232171 1117 265-269 0.8122596847867669 1117 270-274 1.3096753099963898 1117 275-279 0.6012912199213396 1117 280-284 0.26416419393599 1117 285-289 -0.5387373551485606 1117 290-294 0.404867726495171 1117 295-299 -0.1536645243281214 1117 300-301 -0.2634898059003703 2117 1 0.038107564820862194 2117 2 -0.022964152857284148 2117 3 0.06608453326932562 2117 4 0.042362958033180576 2117 5 0.06172230614338048 2117 6 -0.40011661655770325 2117 7 0.09843918108727223 2117 8 0.12434172379023778 2117 9 0.21834584668688706 2117 10-14 0.11769264857620243 2117 15-19 0.11894858067623204 2117 20-24 0.10042055575109288 2117 25-29 0.2023155741738023 2117 30-34 0.17140205640445316 2117 35-39 0.21018426504086563 2117 40-44 0.2592501116878978 2117 45-49 0.2163162887240091 2117 50-54 0.22997152025819645 2117 55-59 0.3025958886089981 2117 60-64 0.367227037762909 2117 65-69 0.38150370766597064 2117 70-74 0.33719349745105376 2117 75-79 0.22786068158599448 2117 80-84 -0.25325481980735987 2117 85-89 0.2502364722741959 2117 90-94 0.37916927757576246 2117 95-99 0.33415261874262825 2117 100-104 0.376196502570771 2117 105-109 0.3099689168754267 2117 110-114 0.34538690131047645 2117 115-119 0.3874946929723606 2117 120-124 0.4453568374833239 2117 125-129 0.505607422780038 2117 130-134 0.45223876586419465 2117 135-139 0.49803069919523324 2117 140-144 0.7120715601552234 2117 145-149 0.8646736066790766 2117 150-154 1.29948812772259 2117 155-159 0.8668029024950812 2117 160-164 0.7260442143296117 2117 165-169 0.8102017058744764 2117 170-174 0.6231570644254276 2117 175-179 1.0457316409429893 2117 180-184 0.8177620510294332 2117 185-189 0.29324424903015966 2117 190-194 0.8719287505597428 2117 195-199 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2110 185-189 -1.4381068526045269 2110 190-194 -0.40361957921992797 2110 195-199 -2.526672279931603 2110 200-204 -0.8174936690778942 2110 205-209 -0.9207062919300562 2110 210-214 -0.7285823440867318 2110 215-219 -0.8535967911732598 2110 220-224 -0.5548996116577172 2110 225-229 -0.09720675277442936 2110 230-234 -0.45426381786313996 2110 235-239 -0.8222860128432181 2110 240-244 -0.12440580657899147 2110 245-249 -0.7654971421183276 2110 250-254 -0.5364634006956592 2110 255-259 -0.6769056924553709 2110 260-264 -0.44872617506564083 2110 265-269 -0.31230112243574837 2110 270-274 -0.40733194792536764 2110 275-279 -0.3128684543572895 2110 280-284 0.052500536651511 2110 285-289 -0.45635392445286094 2110 290-294 -0.1590628806919394 2110 295-299 0.2603825636520529 2110 300-301 -0.06483252142524343 2109 1 -0.11396474435356652 2109 2 -0.014984872458533971 2109 3 -0.0818401906322137 2109 4 -0.009267628510251313 2109 5 -0.017964037628871665 2109 6 -0.4687537861819848 2109 7 -0.18201280361037675 2109 8 -0.09711646701439491 2109 9 -0.1312145249814307 2109 10-14 -0.08035197396124971 2109 15-19 -0.11140444837867136 2109 20-24 -0.02822527031035804 2109 25-29 -0.09341484761807806 2109 30-34 -0.016177526969606504 2109 35-39 -0.04145679299914917 2109 40-44 -0.09067135208771049 2109 45-49 -0.061583966780503374 2109 50-54 -0.17708993346476376 2109 55-59 -0.08185271728575572 2109 60-64 -0.08071060098441762 2109 65-69 -3.117916694997973 2109 70-74 -0.07432951191744053 2109 75-79 -0.28621370792576073 2109 80-84 -0.24756286331292898 2109 85-89 -0.2304429184243233 2109 90-94 -0.24984305086621816 2109 95-99 -0.12002515934542402 2109 100-104 -0.509808167716713 2109 105-109 -0.3742339578994347 2109 110-114 -0.5014826447850567 2109 115-119 -0.2725851696416015 2109 120-124 -0.3152052375484473 2109 125-129 -0.17874624154566732 2109 130-134 -0.15310501336873727 2109 135-139 -0.28161759813942666 2109 140-144 -0.14871582657808347 2109 145-149 -0.32626575637468846 2109 150-154 -0.012956373345502925 2109 155-159 -0.3793697655026236 2109 160-164 -0.47057017629909836 2109 165-169 -0.35804924005071115 2109 170-174 -0.8842342414400122 2109 175-179 -0.9182465853251145 2109 180-184 -0.6824702416698329 2109 185-189 -1.1531608861917668 2109 190-194 -1.1883900367013247 2109 195-199 -1.1275986463621805 2109 200-204 -0.6792696056452279 2109 205-209 -0.633963898059509 2109 210-214 -0.47813715721871475 2109 215-219 -0.5730052280746847 2109 220-224 -0.6227203609115755 2109 225-229 -0.4663433735593223 2109 230-234 -0.5489010845565367 2109 235-239 -0.8007046506444588 2109 240-244 -0.6840243196530729 2109 245-249 -0.4615894402162368 2109 250-254 -0.6561177282826343 2109 255-259 -0.7013759138460784 2109 260-264 -0.2830585729452828 2109 265-269 -0.9034747159143031 2109 270-274 -1.002715973939889 2109 275-279 -0.4531692249929584 2109 280-284 -0.5020993118940797 2109 285-289 -0.8472169473046591 2109 290-294 -0.12947385668196354 2109 295-299 -0.08530998390017785 2109 300-301 -0.5101570347665945 2108 1 0.04924208919710793 2108 2 -0.08951227528454808 2108 3 -0.004518712229256039 2108 4 -0.018036848749048318 2108 5 -0.043689891778832646 2108 6 0.11888313141911055 2108 7 0.026575819658020805 2108 8 0.07792745367738974 2108 9 0.03226697743091478 2108 10-14 -0.008029117826303889 2108 15-19 -0.004217250580239806 2108 20-24 -0.04896478770049839 2108 25-29 -0.11876476521742063 2108 30-34 -0.15565718304557663 2108 35-39 0.02856270585742493 2108 40-44 -0.015170234984005049 2108 45-49 0.015236789410074891 2108 50-54 -0.1763112077527822 2108 55-59 -0.034318857547646076 2108 60-64 -0.06400248535322106 2108 65-69 -0.23947267216097856 2108 70-74 -0.12362844816794905 2108 75-79 -0.2163147825567151 2108 80-84 -0.022139057157190223 2108 85-89 -0.08249573908923935 2108 90-94 0.09490252503452723 2108 95-99 0.006355161376546903 2108 100-104 -0.19573406504145652 2108 105-109 -0.21350739294507548 2108 110-114 -0.2855765973313993 2108 115-119 -0.3350669827017967 2108 120-124 -0.1447761047473648 2108 125-129 -0.1928429846574531 2108 130-134 -0.4087602960495005 2108 135-139 -0.18610444123769554 2108 140-144 -0.3383631798408473 2108 145-149 -0.09689145730681048 2108 150-154 0.24319699893873548 2108 155-159 -0.33293177309506916 2108 160-164 -0.36247507944541013 2108 165-169 -0.1884104164407745 2108 170-174 -0.6061136784829202 2108 175-179 -0.796093356956824 2108 180-184 -0.49039614952501154 2108 185-189 -0.9938234611991774 2108 190-194 -0.577941387492956 2108 195-199 -1.3549553139707307 2108 200-204 -0.6360191760912031 2108 205-209 -0.22826816095534141 2108 210-214 0.286615368769521 2108 215-219 -0.8007065919415979 2108 220-224 -0.31198493284059126 2108 225-229 0.18527015243418532 2108 230-234 -0.0946447702440949 2108 235-239 -0.3446160405391012 2108 240-244 -0.0318865534743793 2108 245-249 0.12057165087729516 2108 250-254 -0.34655363566394115 2108 255-259 -0.8228293947693217 2108 260-264 0.11442079178864262 2108 265-269 -0.4433287289755512 2108 270-274 -0.4382023133323152 2108 275-279 0.1020172777967332 2108 280-284 -0.24397797670505028 2108 285-289 0.117541545930413 2108 290-294 -0.2909928369145778 2108 295-299 0.2559723072417963 2108 300-301 -0.18206025304321827 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 12.0 13 69.0 14 255.0 15 484.0 16 551.0 17 808.0 18 1511.0 19 1525.0 20 1590.0 21 2103.0 22 3074.0 23 4324.0 24 6120.0 25 8384.0 26 11549.0 27 15320.0 28 20905.0 29 28859.0 30 39943.0 31 52825.0 32 63026.0 33 65362.0 34 50908.0 35 20302.0 36 1721.0 37 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.243527290477466 18.63637269258739 13.80338304294552 29.31671697398962 2 49.51236763196955 21.287718039907155 14.738053256029406 14.461861072093882 3 49.44238566291105 19.891067212575837 16.643256328262755 14.023290796250363 4 49.919309046352474 19.580008567187672 16.200701313967507 14.299981072492354 5 34.92299492942032 20.20710678102866 30.915344231593 13.95455405795802 6 33.19087893368399 20.988613609874182 31.141976231035134 14.678531225406694 7 16.927667035254974 50.120787384318064 18.249105924309895 14.702439656117072 8 32.90123825747388 18.80024506141478 32.528914258390365 15.769602422720979 9 15.27997768546467 17.894713248259166 33.92880268571371 32.89650638056244 10-14 27.422720978651764 36.300867676797864 20.595319924688443 15.681091419861929 15-19 23.492673062171882 34.533138081149204 19.20977655579132 22.764412300887603 20-24 25.24242152555712 29.229700248049966 25.16447007959515 20.363408146797767 25-29 21.770469103334232 24.328422143191577 34.906259027923056 18.994849725551138 30-34 21.633792574439894 26.382006913521217 28.59537969576522 23.38882081627367 35-39 21.4647898548559 28.135839733819473 27.375601446460056 23.02376896486457 40-44 23.696193578593984 26.78939661097995 27.053335724176403 22.461074086249663 45-49 23.86549515356186 26.948288056742676 25.059073747546893 24.12714304214857 50-54 23.459898588406404 27.391291354113743 27.02036201348834 22.128448043991515 55-59 22.80420987617425 25.6741181275714 26.90719544145921 24.614476554795132 60-64 23.274907105784845 25.941095603837304 27.03216680115159 23.751830489226265 65-69 20.197593217975154 30.483946484962594 26.13704511720112 23.18141517986113 70-74 21.764491995656634 26.17111463096341 27.86004602372912 24.20434734965084 75-79 21.563461940766864 24.353675423129413 27.0844166840999 26.998445952003824 80-84 27.424912584800214 24.466792185927897 25.71222218901607 22.39607304025582 85-89 22.63929151350328 28.867138858173195 23.8715220704701 24.62204755785342 90-94 22.733929051731867 28.158751979916918 27.267565225187536 21.839753743163683 95-99 22.441598677066835 27.885747586742777 26.025323012860746 23.64733072332965 100-104 22.543209507585946 29.02822191008438 23.58312662502615 24.845441957303528 105-109 25.462428897323253 26.010081388282874 23.67178705557714 24.855702658816732 110-114 20.995786139087297 28.214986601316955 28.1288166322983 22.660410627297452 115-119 25.95945126112117 26.57648862505467 23.355123233140308 24.108936880683864 120-124 20.720789376687286 25.779265413466423 28.48296026219579 25.0169849476505 125-129 23.384188557823535 23.644690834105376 30.391500552882462 22.579620055188627 130-134 25.875011642913464 26.258592719181895 24.721429286547167 23.144966351357475 135-139 25.116964584125096 26.744830670945614 23.479286073979694 24.658918670949596 140-144 22.881713039060397 25.648665610710975 26.393562655031232 25.076058695197396 145-149 21.591504537620914 25.76541844734666 28.46468027454848 24.178396740483947 150-154 23.310719942619766 24.87223932339141 25.885906976280843 25.93113375770798 155-159 23.700887451504464 24.854170952338023 28.31013633291311 23.134805263244406 160-164 26.2091949923742 27.06138196844283 24.910517628002378 21.818905411180587 165-169 23.02148920708148 24.959916001671598 27.25980856310758 24.75878622813934 170-174 23.79451699989042 27.624348744309295 25.45346323580686 23.127671019993425 175-179 23.258071585826283 27.930924558939264 25.146289710409132 23.664714144825318 180-184 21.846239824591727 27.791821220635576 26.93968447829039 23.42225447648231 185-189 22.28514788360579 25.644581253797956 27.41599673251447 24.654274130081788 190-194 20.981192034507835 25.72766305051652 26.311775898309474 26.979369016666165 195-199 20.740723399740695 29.301888418302113 27.738326080275584 22.21906210168161 200-204 23.823866503225897 27.153117137920336 23.890859951814527 25.13215640703924 205-209 22.579371009035395 26.605949214508435 27.48368747696323 23.33099229949294 210-214 24.323690266280146 26.081059541954314 25.49390833109192 24.10134186067362 215-219 23.666618683750578 24.922808106356705 27.10564891747055 24.30492429242217 220-224 19.116294151546654 26.882503453026636 27.608074877257543 26.393127518169173 225-229 21.94929517413067 27.271214882607058 24.565924791012815 26.213565152249462 230-234 22.01632746580596 25.763326459659503 26.727682974208783 25.492663100325753 235-239 21.565614479351325 26.28793037064561 24.592572742681902 27.55388240732116 240-244 22.512726258430213 28.433449886933047 23.838697787473976 25.215126067162767 245-249 21.302909855254377 27.632915931980516 23.157755795303984 27.906418417461126 250-254 20.911757966986443 25.42691491587221 25.437374854307997 28.223952262833347 255-259 21.794427343275256 26.04205891435801 26.147455246406263 26.016058495960472 260-264 21.845741732037162 27.386324071966406 24.40284928864912 26.365084907347313 265-269 20.830618730263094 27.41246027713856 24.7345168006535 27.022404191944847 270-274 18.889353775041588 29.156630106691374 24.809479692776666 27.144536425490372 275-279 20.614745524640625 28.41995158542781 21.947989201358794 29.017313688572766 280-284 18.074325333970894 28.81204984907803 24.840759889622746 28.272864927328335 285-289 19.189803054302025 27.193947182291822 25.648267136865805 27.96798262654035 290-294 19.572836037974557 30.02784335993146 23.787693135291832 26.611627466802148 295-299 19.080222746879453 30.63008676767979 21.60555074066326 28.684139744777504 300-301 14.591365071775101 32.704367273343095 23.839071356703826 28.86519629817798 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.25 20 0.25 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.5 30 1.0 31 2.25 32 4.0 33 6.0 34 13.75 35 39.0 36 98.0 37 437.5 38 1490.0 39 2745.0 40 5085.75 41 6801.25 42 10332.25 43 13163.75 44 15294.75 45 12980.5 46 9222.5 47 7492.0 48 6933.75 49 5864.5 50 5038.25 51 2916.75 52 1442.0 53 1318.75 54 1418.0 55 1733.5 56 2119.5 57 2412.5 58 3267.0 59 4688.75 60 4420.75 61 2998.25 62 1362.75 63 380.5 64 102.75 65 39.75 66 18.75 67 12.0 68 6.0 69 5.0 70 4.0 71 3.5 72 1.0 73 1.5 74 0.5 75 0.25 76 0.75 77 0.25 78 0.25 79 0.25 80 0.0 81 0.25 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.25 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 9.961846129324687E-5 120-124 0.0 125-129 0.0 130-134 4.9809230646623434E-5 135-139 4.9809230646623434E-5 140-144 0.0 145-149 0.0 150-154 0.0 155-159 4.9809230646623434E-5 160-164 9.961846129324687E-5 165-169 4.9809230646623434E-5 170-174 0.0 175-179 0.0 180-184 4.9809230646623434E-5 185-189 0.0 190-194 0.0 195-199 4.9809230646623434E-5 200-204 4.9809230646623434E-5 205-209 0.0 210-214 0.0 215-219 4.9809230646623434E-5 220-224 4.9809230646623434E-5 225-229 4.9809230646623434E-5 230-234 0.0 235-239 4.9809230646623434E-5 240-244 0.0 245-249 0.0 250-254 0.0 255-259 0.0 260-264 4.9809230646623434E-5 265-269 0.0 270-274 0.0 275-279 0.0 280-284 0.0 285-289 0.0 290-294 0.0 295-299 0.0 300-301 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 301 401532.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.239038730271716 #Duplication Level Percentage of deduplicated Percentage of total 1 43.239019017892815 4.859650094010527 2 9.260119091770806 2.081496742386812 3 5.843189300628696 1.9701549257422561 4 4.829703184278921 2.171248845753097 5 4.13559911323254 2.3240079303248935 6 4.211669920188816 2.8401072811273496 7 3.67045143534772 2.8876642087618105 8 2.9644645723646716 2.6654185714659935 9 2.824917234072911 2.857441878320174 >10 17.848432778595498 35.98331220716623 >50 0.8471510384005931 6.251459390634887 >100 0.24539452559284797 4.749212405451217 >500 0.02662959587797032 2.2164997043937333 >1k 0.03772526082665825 10.23949537110101 >5k 0.011095664949017134 8.917377116023516 >10k+ 0.004438265979606853 6.985453327336429 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC 14343 3.5720689758225994 No Hit GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG 13665 3.403215683930546 No Hit CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT 9020 2.246396302162717 No Hit GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG 7684 1.9136706414432725 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA 6759 1.6833029497026388 No Hit GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC 6588 1.640716057499776 No Hit CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT 5703 1.4203102118884672 No Hit CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA 4160 1.0360319974497674 No Hit CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG 4030 1.003655997529462 No Hit GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC 3358 0.8362969825568074 No Hit GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT 3327 0.8285765518065807 No Hit GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA 3230 0.8044190749429685 No Hit CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT 2800 0.6973292290527281 No Hit GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT 2643 0.6582289829951287 No Hit GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG 2519 0.6273472599942221 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA 2456 0.6116573523405358 No Hit GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA 2072 0.5160236294990188 No Hit GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA 2042 0.5085522449020252 No Hit GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA 1778 0.44280406044848225 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT 1520 0.3785501529143381 TruSeq Adapter, Index 8 (97% over 36bp) GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT 1427 0.3553888606636582 No Hit GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 1316 0.3277447376547822 No Hit GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT 1230 0.3063267684767341 No Hit CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGTTTAGCCAAATA 1147 0.2856559377583854 No Hit GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG 905 0.22538676867597104 No Hit GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG 844 0.21019495332875088 No Hit GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA 842 0.20969686102228466 No Hit GGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGT 836 0.20820258410288595 No Hit GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT 807 0.20098024565912553 No Hit CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG 782 0.19475409182829762 No Hit GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA 768 0.191267445683034 No Hit CGGGTTATTAGTAGAAACAAGGGGTCTTACCGAAAGGAAAACTCATCTTG 663 0.16511759959355668 No Hit GGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCT 652 0.1623780919079924 No Hit CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGCCATGGC 599 0.14917864578663717 No Hit GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG 597 0.14868055348017095 No Hit GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 592 0.14743532271400536 No Hit GGGGGGAGCGAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC 495 0.123277845850393 No Hit GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG 453 0.11281790741460207 No Hit GGGGGGAGCAAAAGCAGGCAGCGGAGGCCATGGAGATTGCTAGTCAGGCC 414 0.10310510743851051 No Hit GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT 408 0.1016108305191118 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 2.4904615323311717E-4 0.0 0.0 4 2.4904615323311717E-4 0.0 0.0 5 2.4904615323311717E-4 0.0 0.0 6 2.4904615323311717E-4 0.0 0.0 7 2.4904615323311717E-4 0.0 0.0 8 7.471384596993514E-4 0.0 0.0 9 9.961846129324687E-4 0.0 0.0 10-14 0.001942559995218314 0.0 0.0 15-19 0.0022414153790980542 0.0 0.0 20-24 0.002341033840391301 0.0 0.0 25-29 0.0026896984549176655 0.0 0.0 30-34 0.008517378440572607 0.0 0.0 35-39 0.009214707669625335 0.0 0.0 40-44 0.011107458434197024 0.0 0.0 45-49 0.01628761842144586 0.0 0.0 50-54 0.02799278762340237 0.0 0.0 55-59 0.032226572228365356 0.0 0.0 60-64 0.03929948298018589 0.0 0.0 65-69 0.047617624498172 0.0 0.0 70-74 0.06614665829871592 0.0 0.0 75-79 0.08826195670581673 0.0 0.0 80-84 0.11167229510972973 0.0 0.0 85-89 0.14628971040913302 0.0 0.0 90-94 0.19121763645238737 0.0 0.0 95-99 0.23759003018439379 0.0 0.0 100-104 0.30079794387495895 0.0 0.0 105-109 0.37207495293027704 0.0 0.0 110-114 0.4602372911748005 0.0 0.0 115-119 0.5615492663100325 0.0 0.0 120-124 0.6760606875666199 0.0 0.0 125-129 0.8177679487562635 0.0 0.0 130-134 0.9978781267744539 0.0 0.0 135-139 1.2102148770210097 0.0 0.0 140-144 1.4488509010489825 0.0 0.0 145-149 1.7650897064243947 0.0 0.0 150-154 2.1215245609316318 0.0 0.0 155-159 2.5293625414661847 0.0 0.0 160-164 3.0058874510624305 0.0 0.0 165-169 3.5719693573613065 0.0 0.0 170-174 4.205243915802476 0.0 0.0 175-179 4.969118276999093 0.0 4.9809230646623434E-5 180-184 5.871910582469143 0.0 2.4904615323311717E-4 185-189 6.9096859029915425 0.0 2.4904615323311717E-4 190-194 8.083838897024396 0.0 2.4904615323311717E-4 195-199 9.244393971090721 0.0 2.4904615323311717E-4 200-204 10.494755088012912 0.0 2.4904615323311717E-4 205-209 11.871781078469462 0.0 2.4904615323311717E-4 210-214 13.375920225536197 0.0 2.4904615323311717E-4 215-219 14.790004283593836 0.0 2.4904615323311717E-4 220-224 16.27760676608589 0.0 2.4904615323311717E-4 225-229 17.77561937778309 0.0 2.4904615323311717E-4 230-234 19.341571780082283 0.0 2.4904615323311717E-4 235-239 20.81781775798691 0.0 2.4904615323311717E-4 240-244 22.247492105236944 0.0 2.4904615323311717E-4 245-249 23.527439905163227 0.0 2.4904615323311717E-4 250-254 24.8495761234472 0.0 2.4904615323311717E-4 255-259 26.055507406632596 0.0 2.4904615323311717E-4 260-264 27.225526234521784 0.0 2.4904615323311717E-4 265-269 28.282029825767314 0.0 2.4904615323311717E-4 270-274 29.43053106601716 0.0 2.4904615323311717E-4 275-279 30.278035125469454 0.0 2.4904615323311717E-4 280-284 30.997280416006696 0.0 2.4904615323311717E-4 285-289 31.682406383551 0.0 2.4904615323311717E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTTA 5615 0.0 291.58505 1 GGGTTAT 5645 0.0 289.77414 2 GTTATTA 5690 0.0 287.48242 4 GGTTATT 5705 0.0 286.72656 3 TTATTAG 5785 0.0 282.76147 5 GGGGGGA 7245 0.0 279.12006 1 TTAGTAG 5985 0.0 273.06598 8 TATTAGT 6045 0.0 270.35565 6 ATTAGTA 6185 0.0 264.47455 7 GGAGCGA 5550 0.0 262.84235 5 GGGAGCG 5580 0.0 261.69354 4 TAGTAGA 6310 0.0 259.0016 9 GGGGAGC 7825 0.0 257.11182 3 CGAAAGC 5690 0.0 255.59753 9 GCGAAAG 5850 0.0 248.60683 8 AGCGAAA 5950 0.0 244.92436 7 GAGCGAA 6060 0.0 240.96533 6 GGGGGAG 8485 0.0 238.3294 2 GGGAGCA 2550 0.0 216.33334 4 AAACGTT 140 0.0 210.71428 295 >>END_MODULE