##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_30429_flu.3510000003bb0e.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398481
Sequences flagged as poor quality	0
Sequence length	301
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.81359462559068	34.0	34.0	34.0	34.0	34.0
2	33.885043452510914	34.0	34.0	34.0	34.0	34.0
3	33.8856457396965	34.0	34.0	34.0	34.0	34.0
4	33.887655873178396	34.0	34.0	34.0	34.0	34.0
5	33.876192340412715	34.0	34.0	34.0	34.0	34.0
6	37.60591094682055	38.0	38.0	38.0	38.0	38.0
7	37.63034373031587	38.0	38.0	38.0	38.0	38.0
8	37.647875306476344	38.0	38.0	38.0	38.0	38.0
9	37.55834280680886	38.0	38.0	38.0	37.0	38.0
10-14	37.642541049635994	38.0	38.0	38.0	37.6	38.0
15-19	37.60540502558466	38.0	38.0	38.0	37.2	38.0
20-24	37.59366695024355	38.0	38.0	38.0	37.0	38.0
25-29	37.50001681385059	38.0	38.0	38.0	37.0	38.0
30-34	37.44789989986976	38.0	38.0	38.0	37.0	38.0
35-39	37.36995139040506	38.0	38.0	38.0	37.0	38.0
40-44	37.38096872874742	38.0	38.0	38.0	37.0	38.0
45-49	37.3520474000015	38.0	38.0	38.0	37.0	38.0
50-54	37.303969825412004	38.0	38.0	38.0	37.0	38.0
55-59	37.24379832413591	38.0	38.0	38.0	37.0	38.0
60-64	37.08347600011042	38.0	38.0	38.0	36.4	38.0
65-69	37.00160308772564	38.0	38.0	38.0	36.0	38.0
70-74	37.08895480587531	38.0	38.0	38.0	36.2	38.0
75-79	37.00909001934848	38.0	38.0	38.0	36.0	38.0
80-84	36.888644627974735	38.0	38.0	38.0	35.8	38.0
85-89	36.926491852811054	38.0	38.0	38.0	36.0	38.0
90-94	36.779041911659526	38.0	38.0	38.0	35.4	38.0
95-99	36.77643300433395	38.0	38.0	38.0	35.2	38.0
100-104	36.73170565221428	38.0	38.0	38.0	35.2	38.0
105-109	36.5656831818832	38.0	38.0	38.0	34.2	38.0
110-114	36.48014585388011	38.0	37.0	38.0	34.0	38.0
115-119	36.554296942639674	38.0	37.2	38.0	34.0	38.0
120-124	36.44202107503243	38.0	37.0	38.0	34.0	38.0
125-129	36.35187926149553	38.0	37.0	38.0	34.0	38.0
130-134	36.1899749297959	38.0	37.0	38.0	33.4	38.0
135-139	35.90677046082498	38.0	37.0	38.0	31.6	38.0
140-144	35.875717035442094	38.0	37.0	38.0	31.6	38.0
145-149	35.4194483551286	38.0	36.2	38.0	28.6	38.0
150-154	35.00860718578803	38.0	35.4	38.0	27.0	38.0
155-159	35.10753636936265	38.0	35.6	38.0	27.6	38.0
160-164	35.01708487983116	38.0	35.8	38.0	27.0	38.0
165-169	34.738830709619776	38.0	35.2	38.0	25.4	38.0
170-174	34.659175719795925	38.0	35.0	38.0	25.0	38.0
175-179	34.26923391579523	37.8	34.4	38.0	23.4	38.0
180-184	33.98085780752408	37.2	34.0	38.0	23.0	38.0
185-189	33.1573299605251	37.0	32.8	37.8	21.6	38.0
190-194	32.158524998682495	36.8	29.8	37.0	20.2	38.0
195-199	31.872138445747726	35.8	29.2	37.2	19.8	37.8
200-204	31.721982729414954	36.8	29.0	37.0	19.4	37.4
205-209	30.599104599717425	34.8	26.4	37.0	16.4	37.2
210-214	29.862594201480125	33.6	25.0	37.0	15.4	37.0
215-219	29.042416576950973	32.6	23.4	36.6	13.4	37.0
220-224	27.514343218371767	31.0	20.8	36.2	9.4	37.0
225-229	26.491418662370354	29.0	19.8	35.4	9.0	37.0
230-234	26.130078974907214	28.6	19.2	35.0	9.0	37.0
235-239	25.01598068665758	27.0	17.8	33.6	8.8	37.0
240-244	24.06200897909813	25.6	16.6	32.8	8.0	37.0
245-249	23.251882021978464	24.0	15.2	32.0	8.0	36.2
250-254	22.343776993131414	22.6	13.4	31.2	8.0	36.0
255-259	21.858364137813346	22.0	13.0	30.4	8.0	36.0
260-264	20.639216926277538	20.2	11.4	29.2	7.8	35.0
265-269	19.842033120776147	19.4	10.2	27.6	7.0	33.8
270-274	18.947743556154496	18.6	8.8	26.6	7.0	32.6
275-279	17.770532095633165	17.0	8.0	24.8	7.0	31.2
280-284	17.549475633719048	16.6	8.0	24.4	7.0	30.6
285-289	16.45036977923665	14.6	8.0	22.6	7.0	29.4
290-294	15.769138804610506	13.4	8.0	21.2	7.0	28.4
295-299	14.979174916746341	13.0	8.0	20.2	7.0	27.0
300-301	13.166172289268498	11.0	8.0	16.5	7.0	22.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.044721498843742324
1101	2	0.07166789334578283
1101	3	0.00873879483557971
1101	4	0.028351748793241427
1101	5	0.049331094868541925
1101	6	0.19791822229301914
1101	7	0.10587432948371145
1101	8	0.1277299348953349
1101	9	0.09311150619060271
1101	10-14	0.06515673748963025
1101	15-19	0.09213525256198096
1101	20-24	0.0657493323707925
1101	25-29	0.09188274765338633
1101	30-34	0.1275349256043512
1101	35-39	0.07390256586456445
1101	40-44	0.10901650874546931
1101	45-49	0.07781503569287906
1101	50-54	0.15835776805084123
1101	55-59	0.10969637277437982
1101	60-64	0.27814568282554575
1101	65-69	0.24524019475057912
1101	70-74	0.20422078784626763
1101	75-79	0.0637413838481109
1101	80-84	0.14944821526894714
1101	85-89	0.17090666839501978
1101	90-94	0.3004103701741698
1101	95-99	0.05363356052843926
1101	100-104	0.16234884373463387
1101	105-109	0.08339995313865245
1101	110-114	0.09559547310333727
1101	115-119	0.11625777280897154
1101	120-124	0.15034234356581777
1101	125-129	0.2196894341694886
1101	130-134	0.08066461882001619
1101	135-139	0.22845473427659613
1101	140-144	0.19835961787815393
1101	145-149	0.5066922529706162
1101	150-154	0.7389309639521642
1101	155-159	0.39328327810834196
1101	160-164	0.25488809138223445
1101	165-169	0.3366545664077236
1101	170-174	0.24465785965880826
1101	175-179	0.19791960926771424
1101	180-184	0.1595126444458188
1101	185-189	0.2899621755731374
1101	190-194	0.4932123232980743
1101	195-199	0.16862810556873242
1101	200-204	-0.1938745893101732
1101	205-209	-0.34616392128324236
1101	210-214	-0.30450542905066413
1101	215-219	-0.19786882995153832
1101	220-224	0.27968014735715485
1101	225-229	0.4872307287596307
1101	230-234	0.31587893238896214
1101	235-239	0.5135805581077157
1101	240-244	0.5648762392149429
1101	245-249	0.5068846195289467
1101	250-254	0.3895844953759635
1101	255-259	-0.09803326174311522
1101	260-264	0.4276480729521559
1101	265-269	0.046525387185862854
1101	270-274	0.1905181646870986
1101	275-279	0.8343225671238876
1101	280-284	0.1358743165352685
1101	285-289	0.1437405771742455
1101	290-294	0.06098266416121412
1101	295-299	0.05153158478756836
1101	300-301	0.0648434476998645
1102	1	-0.11566246165813965
1102	2	-0.05303385949341788
1102	3	0.03170221903442183
1102	4	0.019775822734658277
1102	5	-5.898317502541772E-4
1102	6	-0.1355188891132002
1102	7	0.06751134133645564
1102	8	-0.016191884398566003
1102	9	-0.01660946855183454
1102	10-14	0.030326061058637777
1102	15-19	-0.015052802489996964
1102	20-24	0.013421489173026657
1102	25-29	-0.057566861204300324
1102	30-34	-0.030048863709637885
1102	35-39	-0.03191304713322296
1102	40-44	-0.1406423093600324
1102	45-49	-0.09053467253461633
1102	50-54	-0.04073835248601654
1102	55-59	-0.10596034038118773
1102	60-64	0.05777781251690328
1102	65-69	0.24617511579658924
1102	70-74	0.048035890219189525
1102	75-79	-0.016468964203340875
1102	80-84	0.028532984505083903
1102	85-89	0.1682148672301338
1102	90-94	0.12629831557037363
1102	95-99	0.018767434242462855
1102	100-104	0.09284086200691632
1102	105-109	0.03222211793087837
1102	110-114	0.24316421918869224
1102	115-119	-0.01889213599490347
1102	120-124	0.07069147871794002
1102	125-129	0.11845530165315665
1102	130-134	0.1205089700784896
1102	135-139	0.18409245512643224
1102	140-144	0.25525865558908833
1102	145-149	0.27118380470076886
1102	150-154	0.7597181711464742
1102	155-159	0.4275412465193966
1102	160-164	0.5588748224831548
1102	165-169	0.5108812360867034
1102	170-174	0.43446575083326877
1102	175-179	0.39742018877181806
1102	180-184	0.5013934337469408
1102	185-189	0.5616087203143394
1102	190-194	0.7627575916608222
1102	195-199	0.730509409168274
1102	200-204	0.18566202781853391
1102	205-209	0.00470643694396955
1102	210-214	-0.3735797470083142
1102	215-219	-0.2360051594404844
1102	220-224	0.8534213560686368
1102	225-229	1.1441415218628919
1102	230-234	0.7860393383908217
1102	235-239	1.0558992357730226
1102	240-244	0.940108178615894
1102	245-249	1.2675284844080572
1102	250-254	0.6384219054426161
1102	255-259	0.35954520090871966
1102	260-264	1.0901516776330098
1102	265-269	0.7746701842308497
1102	270-274	0.9772404490537134
1102	275-279	1.327622544531497
1102	280-284	0.5517114014918576
1102	285-289	1.0923709821291467
1102	290-294	1.052855096113106
1102	295-299	1.3238061246282502
1102	300-301	0.5391377108847308
1103	1	-0.07462417229311313
1103	2	0.005503017380846131
1103	3	0.0017719019121358315
1103	4	0.004131582075203255
1103	5	0.011845519633986612
1103	6	0.10099544585717268
1103	7	0.09069248784434336
1103	8	0.1063579873907301
1103	9	0.18268392388880272
1103	10-14	0.08856015786134463
1103	15-19	-0.0029332695536083975
1103	20-24	0.07746708002878222
1103	25-29	0.07149060906095173
1103	30-34	0.15031171983013536
1103	35-39	-0.32275250125987043
1103	40-44	-0.2189066117344609
1103	45-49	-0.20923170413094994
1103	50-54	0.04857706032346698
1103	55-59	-0.3347179279918322
1103	60-64	0.13948605711684792
1103	65-69	0.2785316574397143
1103	70-74	0.09268617199299456
1103	75-79	0.18433905294936892
1103	80-84	0.12751128285211166
1103	85-89	0.23665439089478468
1103	90-94	0.2779842500597809
1103	95-99	0.21607935566451175
1103	100-104	0.18713005319297338
1103	105-109	-0.016716340933037088
1103	110-114	0.11775495904411315
1103	115-119	0.03350692524200838
1103	120-124	0.3352162299022652
1103	125-129	0.21929772563217398
1103	130-134	0.38244379268413553
1103	135-139	0.3044732481494563
1103	140-144	0.25959355352757285
1103	145-149	0.6950096264806547
1103	150-154	0.8620976748189406
1103	155-159	-0.23733843824163614
1103	160-164	-0.6505693080744521
1103	165-169	0.3842528877592386
1103	170-174	0.6840709777734517
1103	175-179	0.5021212477777155
1103	180-184	0.45480622874021037
1103	185-189	0.6080981374989207
1103	190-194	0.4141436434956063
1103	195-199	0.4051718600932652
1103	200-204	0.48821827392513484
1103	205-209	0.9500039292295988
1103	210-214	0.7765854057579915
1103	215-219	0.4348022782288261
1103	220-224	0.9917827943464061
1103	225-229	2.089016353450532
1103	230-234	1.4310021790184067
1103	235-239	1.4892553862648583
1103	240-244	1.4168788625511652
1103	245-249	1.3596507698905462
1103	250-254	1.645001577165278
1103	255-259	0.3779495933928203
1103	260-264	2.108852693177653
1103	265-269	1.0053074710896155
1103	270-274	1.1409568209082046
1103	275-279	1.9862637284160662
1103	280-284	1.0532202313700232
1103	285-289	0.9869415849367371
1103	290-294	1.3147203807118863
1103	295-299	1.3469934281471598
1103	300-301	-0.879855943772144
2116	1	0.0762353197484984
2116	2	0.02096701648056154
2116	3	0.023814848380268927
2116	4	0.06636826592702505
2116	5	0.04698042190369023
2116	6	-0.20635472329817617
2116	7	-0.06519330943651624
2116	8	0.11215182183652672
2116	9	0.21104733709909596
2116	10-14	0.12926804310298223
2116	15-19	0.17925354687942985
2116	20-24	-0.023907439056578994
2116	25-29	-0.09981944812129484
2116	30-34	0.040456046971655724
2116	35-39	-0.04245663765323826
2116	40-44	0.02624731611602016
2116	45-49	0.2197947910124327
2116	50-54	0.32903856253550146
2116	55-59	0.2747642667372787
2116	60-64	0.5103454436561563
2116	65-69	0.397497277758049
2116	70-74	-0.6340989268626558
2116	75-79	0.3676589640461003
2116	80-84	-0.03619680636063549
2116	85-89	0.3174056727476895
2116	90-94	0.445453847814683
2116	95-99	0.45537016922788354
2116	100-104	0.418185709171091
2116	105-109	0.4702739950566084
2116	110-114	0.6153057232324173
2116	115-119	0.5678363174784451
2116	120-124	0.16731249019752426
2116	125-129	0.25272120481656657
2116	130-134	0.4520934195576771
2116	135-139	0.6452725687146668
2116	140-144	0.8253666743558412
2116	145-149	0.961472028978946
2116	150-154	1.2685217096487662
2116	155-159	0.21338741755782564
2116	160-164	-0.2619895799890415
2116	165-169	0.9862892579175693
2116	170-174	1.2380916702905225
2116	175-179	1.0613066293795228
2116	180-184	1.0032200017133306
2116	185-189	1.4367618805797377
2116	190-194	1.4953587837784923
2116	195-199	1.2778673382648442
2116	200-204	1.1903434418496133
2116	205-209	1.2721991506789152
2116	210-214	1.1745474530652231
2116	215-219	1.448981259357982
2116	220-224	1.7650898341629748
2116	225-229	1.5406138340556907
2116	230-234	1.6780755453153482
2116	235-239	1.338486098686701
2116	240-244	2.268812367970469
2116	245-249	1.3172284723318732
2116	250-254	1.5112730205070228
2116	255-259	1.505578534782778
2116	260-264	0.8753583516174821
2116	265-269	1.0884264003116435
2116	270-274	1.4281442703550233
2116	275-279	1.9038771609684453
2116	280-284	0.3314540525928642
2116	285-289	-0.9219084751995723
2116	290-294	0.721696016714894
2116	295-299	1.0915333326324141
2116	300-301	1.8869074919453102
1117	1	-0.014473796353264845
1117	2	0.03369850610265246
1117	3	-0.010446143200645963
1117	4	0.01540977756393147
1117	5	-0.016349969087819716
1117	6	-0.551260193240573
1117	7	-0.4187060083962635
1117	8	-0.15961945621829443
1117	9	-0.013744647539766675
1117	10-14	0.0671315864327724
1117	15-19	0.05139003871706649
1117	20-24	-0.005803596662943278
1117	25-29	0.07712970680531583
1117	30-34	0.016289163439161314
1117	35-39	-0.22500814035760897
1117	40-44	-0.16439533353897673
1117	45-49	0.019527694365287118
1117	50-54	4.5675957159119207E-4
1117	55-59	0.17280086900065328
1117	60-64	0.1763281623800026
1117	65-69	0.18586248450738196
1117	70-74	-0.11257698590173959
1117	75-79	0.1850909326486203
1117	80-84	-0.11064661188473934
1117	85-89	-0.0499997444435607
1117	90-94	0.1811797387815801
1117	95-99	0.19709439325850298
1117	100-104	0.12923531635087215
1117	105-109	0.20226862147297453
1117	110-114	0.1741459364877258
1117	115-119	0.21508587261042322
1117	120-124	0.1970583351654156
1117	125-129	0.21553832713593124
1117	130-134	0.14522574757135942
1117	135-139	0.2559770075479548
1117	140-144	0.2629770121741899
1117	145-149	0.6200096264806518
1117	150-154	1.0023232387287209
1117	155-159	-0.20256400215141923
1117	160-164	-0.7349678043150618
1117	165-169	0.16507995542841059
1117	170-174	0.3579431582245789
1117	175-179	0.6919708718378672
1117	180-184	0.5813099881386989
1117	185-189	0.7302785886267458
1117	190-194	0.7915872525181626
1117	195-199	0.7984049428000368
1117	200-204	0.7511881987371609
1117	205-209	1.066733252537869
1117	210-214	0.7534651050061143
1117	215-219	0.36168197747695174
1117	220-224	0.9056925687825057
1117	225-229	0.5715351504430117
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2108	185-189	-1.09900403958558
2108	190-194	-0.5178056326799023
2108	195-199	-1.6598110262270076
2108	200-204	-1.1577193312819212
2108	205-209	-0.564844292069921
2108	210-214	-0.3302109695085278
2108	215-219	-1.089739019044199
2108	220-224	-0.6776720928716351
2108	225-229	-0.166270651374969
2108	230-234	-0.191838972367524
2108	235-239	-0.729136208393431
2108	240-244	-0.6829093205932608
2108	245-249	-0.25419167203516224
2108	250-254	-0.671723537861098
2108	255-259	-0.8298707971357366
2108	260-264	-0.19608081596834737
2108	265-269	-0.9411042672113581
2108	270-274	-1.0335157410938152
2108	275-279	-0.5101858844220004
2108	280-284	-0.7437470001415925
2108	285-289	-0.06740276474343077
2108	290-294	-0.6910155075161448
2108	295-299	-0.2544794705174116
2108	300-301	-0.3477923426050449
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	9.0
14	39.0
15	110.0
16	246.0
17	481.0
18	1217.0
19	1212.0
20	1325.0
21	1717.0
22	2569.0
23	3716.0
24	5475.0
25	7594.0
26	9921.0
27	13286.0
28	18262.0
29	24958.0
30	35375.0
31	49351.0
32	64208.0
33	73307.0
34	59164.0
35	23083.0
36	1853.0
37	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.83026794251169	19.12161433041977	14.190638951418011	31.857478775650534
2	49.58078302353186	21.307665861107555	15.084282563033119	14.027268552327463
3	49.47789229599404	19.829552726478802	16.956642851227535	13.735912126299624
4	49.77552254687175	19.785134046541742	16.643955420710146	13.795387985876365
5	32.279330758555616	19.9384663258725	34.29548711230899	13.486715803262891
6	30.489534005385448	20.691827213844576	34.51155764013842	14.307081140631547
7	16.23519373619755	51.31248745231881	18.33818510339289	14.114133708090746
8	30.083492061102035	18.989613055578562	35.65941663467016	15.267478248649248
9	14.841861970834245	17.75040717123276	37.236907154920814	30.17082370301219
10-14	27.589972922171945	35.817667592683215	21.491865358699663	15.100494126445174
15-19	22.46696831216545	36.15770889954602	19.668039379543817	21.707283408744708
20-24	25.935540213962522	30.4683033820935	25.676004627573207	17.92015177637077
25-29	20.171400894898376	25.5722606598558	36.77485250237778	17.48148594286804
30-34	19.849628965998377	27.60262095306928	30.839814194403246	21.707935886529096
35-39	20.773537508689248	29.652354817419145	27.418722599069966	22.155385074821634
40-44	21.052697619208946	28.81638020382402	28.185032661532166	21.945889515434864
45-49	22.697192588856183	28.89628363711193	25.825271468401255	22.581252305630635
50-54	22.814136684057708	29.412343373962624	26.589072000923508	21.184447941056163
55-59	22.356950519598172	26.511176191587555	28.76312797849835	22.368745310315923
60-64	22.55013413437529	25.661549735119117	29.03375568722223	22.75456044328337
65-69	20.54075351145977	27.81588080736597	27.4267530948778	24.21661258629646
70-74	21.0974174427388	25.519159006326525	28.8175345875964	24.565888963338278
75-79	20.406995565660598	24.85604081499494	29.715946306097408	25.021017313247057
80-84	24.92279431139753	25.208077674970703	26.475490675841506	23.393637337790256
85-89	21.974447966151462	27.525327430918917	25.915062449652552	24.58516215327707
90-94	20.880644246526185	29.48160640030516	28.89101362423804	20.746735728930616
95-99	22.16607567236581	29.366820500851986	26.62681533122031	21.840288495561897
100-104	22.5495820377885	28.170979293868463	25.69487629272161	23.584562375621424
105-109	23.838878139735645	27.50133632469302	23.50867419023743	25.151111345333906
110-114	21.11779482585117	28.862505364120246	28.763077787899547	21.256622022129037
115-119	24.84680574481594	27.0151901847265	25.94557833372231	22.192425736735252
120-124	19.557419299790958	27.55960760989859	27.601065044506512	25.28190804580394
125-129	20.814994943297172	24.030656417746393	31.346488289278536	23.807860349677902
130-134	25.923243517256783	28.15833126297113	25.384999535736963	20.533425684035123
135-139	25.000790501931082	26.940908098504067	24.42901920041357	23.629282199151277
140-144	21.466970821695387	28.005249936634367	26.72815015019537	23.799629091474873
145-149	21.64063029353972	26.940657145510073	28.658179436409764	22.76053312454044
150-154	23.353133524559514	25.30484514945506	25.797666639061838	25.544354686923594
155-159	22.48443464054748	25.517352144769763	29.246212491938135	22.752000722744622
160-164	25.607243507218662	29.345690258757635	25.18353447215802	19.863531761865687
165-169	21.96265317543371	25.61913867913401	28.00279059729322	24.415417548139057
170-174	23.33732348593785	28.607286169227642	25.6244086920079	22.43098165282661
175-179	20.78613534898778	28.838614639091954	27.813672421018822	22.56157759090145
180-184	20.657195700673306	29.87530145728404	26.658184455469648	22.80931838657301
185-189	22.29867923439261	27.511324253854006	27.231260712555933	22.958735799197452
190-194	20.47018552954846	27.044652066221474	26.657582168284062	25.82758023594601
195-199	20.8041537739566	30.715542271777075	27.08475435466183	21.3955495996045
200-204	22.892032493393664	27.227345845849616	26.58330008206163	23.297321578695094
205-209	21.168788474230894	27.558854750916606	27.89332490131273	23.37903187353977
210-214	23.698846369086606	26.36070477638833	27.828328075868107	22.112120778656948
215-219	23.746878772137194	25.928814673723465	28.22809619530166	22.096210358837688
220-224	18.75346628822955	27.112459565198844	28.888504094298096	25.245570052273507
225-229	21.944885703458887	28.387350965290693	25.885751139953978	23.78201219129645
230-234	21.51148988283005	26.765140621510184	28.610598748748373	23.112770746911394
235-239	23.115129705054947	25.977599935756036	25.376165990348348	25.531104368840673
240-244	22.63661253610586	30.738981281416176	23.636459454779526	22.987946727698436
245-249	21.205477801952917	28.671981851079476	23.93669961679478	26.18584073017283
250-254	21.513447316183207	25.963496377493534	26.128824209937235	26.394232096386027
255-259	21.988551524413964	26.3763642432136	27.09644876418198	24.538635468190453
260-264	21.265455567517648	27.89683824322866	26.421234638539854	24.416471550713837
265-269	20.970886943166676	27.428258812841765	26.630278482537435	24.97057576145412
270-274	19.125077481736895	29.565675653293383	25.31327717005328	25.995969694916443
275-279	21.247939048536818	27.45380582763043	23.731771401898712	27.56648372193404
280-284	18.202574275812395	28.767093035803466	26.716907456064405	26.31342523231973
285-289	20.429882478712912	26.054943648505198	28.349105729005903	25.166068143775988
290-294	20.56098032277574	29.867772867464193	25.443421392738923	24.127825417021135
295-299	19.882553998810483	30.105626115172367	23.07889209272211	26.932927793295036
300-301	15.556701574228132	31.747184934789864	26.728501484386957	25.967612006595044
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.25
6	0.75
7	0.25
8	1.0
9	0.5
10	0.25
11	0.5
12	0.75
13	1.5
14	1.75
15	2.5
16	2.25
17	2.5
18	3.25
19	1.75
20	1.5
21	1.5
22	1.75
23	0.5
24	1.75
25	2.75
26	1.5
27	1.0
28	1.25
29	1.0
30	1.0
31	1.5
32	4.5
33	11.5
34	26.5
35	79.5
36	262.25
37	978.75
38	3137.25
39	3787.25
40	5663.0
41	7085.25
42	10508.0
43	14902.25
44	17844.0
45	13981.25
46	9913.75
47	8686.0
48	7927.5
49	6602.75
50	5774.25
51	3296.5
52	1248.5
53	786.75
54	1075.5
55	1189.25
56	1163.5
57	1085.75
58	989.75
59	1451.0
60	1596.0
61	952.75
62	343.5
63	111.25
64	62.75
65	56.0
66	41.5
67	17.75
68	6.25
69	2.0
70	1.0
71	0.5
72	0.5
73	1.0
74	0.25
75	0.0
76	0.25
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.25
98	0.25
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.5095299399469485E-4
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	398481.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.056514782258207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	40.92748848789637	4.525153814671288
2	9.997919600439161	2.210842917081693
3	8.028895459492055	2.6631480399923912
4	5.875173848034372	2.5983578599651542
5	4.332196895191378	2.3949499505668292
6	4.416595413233286	2.929929148420057
7	3.0831118778719824	2.38619304371332
8	2.5813928896428617	2.283296691452202
9	2.3466974952203237	2.3351665990852633
>10	17.060980321031685	34.616688425845396
>50	1.0295215466118879	7.694321548287503
>100	0.24095521532405315	4.225226821379468
>500	0.022591700005198306	1.5711505752190225
>1k	0.04292423000407277	11.843078253469432
>5k	0.0067775100006430685	5.516761780653531
>10k+	0.0067775100006430685	10.2057345301975
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	19823	4.974641199956836	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	10585	2.6563374414338448	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	10450	2.6224587872445615	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	9871	2.477157003721633	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	6808	1.7084879831158826	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	5407	1.356902838529315	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	4929	1.236947307399851	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	4715	1.1832433666849862	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	4203	1.0547554337597025	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	3680	0.9235070179004771	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3402	0.8537420855699519	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	3072	0.7709275975517026	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2974	0.7463342041402224	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2815	0.706432678095066	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	2592	0.6504701604342491	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC	2525	0.6336563098366045	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1776	0.4456925173345781	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1690	0.4241105598510343	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	1583	0.39725858949360193	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1507	0.37818616195000515	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1430	0.3588627814124136	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT	1294	0.3247331742291351	TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	1105	0.2773030583641378	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1097	0.27529543441218024	No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	1024	0.25697586585056753	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	921	0.23112770746911396	No Hit
GGGGGGAGCGAAAGCATGGGTAGCAAACAGGATTAGATACCCTGGTAGTC	722	0.18118806166416968	No Hit
CGGGTTATTAGTAGAAACAAGGACACCTACCTTAAGGATTTGGTGTGAGG	652	0.16362135208454104	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGGCGTGCT	640	0.16060991615660472	No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG	639	0.16035896316261	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	589	0.14781131346287527	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	537	0.13476175777515112	No Hit
GGGGGGAGCGAAAGCAGGGGTAGCGAACAGGATTAGATACCCTGGTAGTC	535	0.13425985178716177	No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT	529	0.13275413382319357	No Hit
GGGGGGAGCGAAAGCAGGGGTAGCAAACAGGATTAGATACCCTGGTAGTC	526	0.1320012748412095	No Hit
GGGGGGAGCGAAAGCAGGTGGATCATAAAGTCAGCAGTTCAAGACCAGCC	500	0.12547649699734742	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	492	0.12346887304538987	No Hit
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG	491	0.12321792005139517	No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC	433	0.10866264639970288	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	2.5095299399469485E-4	0.0	0.0
9	2.5095299399469485E-4	0.0	0.0
10-14	0.0012045743711745353	0.0	0.0
15-19	0.0016060991615660472	0.0	0.0
20-24	0.002007623951957559	0.0	0.0
25-29	0.002007623951957559	0.0	0.0
30-34	0.007127065029449334	0.0	0.0
35-39	0.007578780418639785	0.0	0.0
40-44	0.009134688981406892	0.0	0.0
45-49	0.012898983891327315	0.0	0.0
50-54	0.02133100448954906	0.0	0.0
55-59	0.025145489998268423	0.0	0.0
60-64	0.029913596884167625	0.0	0.0
65-69	0.03488246616526259	0.0	0.0
70-74	0.04286277137429388	0.0	0.0
75-79	0.05375413131366365	0.0	0.0
80-84	0.07046760071371032	0.0	0.0
85-89	0.0875825949041485	0.0	0.0
90-94	0.12311753885379731	0.0	0.0
95-99	0.15303113573796492	0.0	0.0
100-104	0.19478971393868214	0.0	0.0
105-109	0.23900763148054738	0.0	0.0
110-114	0.27725286776533886	0.0	0.0
115-119	0.3201156391396328	0.0	0.0
120-124	0.3739199610520953	0.0	0.0
125-129	0.43941869248471066	0.0	0.0
130-134	0.5236385172693302	0.0	0.0
135-139	0.6192516079813091	0.0	0.0
140-144	0.7330838860573026	0.0	0.0
145-149	0.8778837635922416	0.0	0.0
150-154	1.0505896140593907	0.0	0.0
155-159	1.280412365959732	0.0	0.0
160-164	1.5250915351045595	0.0	0.0
165-169	1.8042014550254593	0.0	0.0
170-174	2.126977195901436	0.0	0.0
175-179	2.5081747937793772	0.0	0.0
180-184	3.0011468551825558	0.0	0.0
185-189	3.8328552678797734	0.0	0.0
190-194	4.465457575141601	0.0	0.0
195-199	5.117082119348224	0.0	0.0
200-204	5.816287351216244	0.0	0.0
205-209	6.621394746550024	0.0	0.0
210-214	7.469113960264103	0.0	0.0
215-219	8.33254283140225	0.0	0.0
220-224	9.31974171917858	0.0	0.0
225-229	10.399642642936552	0.0	0.0
230-234	11.520950810703647	0.0	0.0
235-239	12.65656329912844	0.0	0.0
240-244	13.8127539330608	0.0	0.0
245-249	14.88793694053167	0.0	0.0
250-254	16.055269887397394	0.0	0.0
255-259	17.19394400234892	0.0	0.0
260-264	18.329205156582123	0.0	0.0
265-269	19.364787781600633	0.0	0.0
270-274	20.360117546382387	0.0	0.0
275-279	21.240611221112175	0.0	0.0
280-284	22.050687485727046	0.0	0.0
285-289	22.808565527591025	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	6740	0.0	292.59274	1
GGGTTAT	6825	0.0	289.16483	2
GGGGGGA	5940	0.0	286.30896	1
GTTATTA	6940	0.0	284.16064	4
GGTTATT	6990	0.0	282.12805	3
TTATTAG	7005	0.0	281.31335	5
TTAGTAG	7065	0.0	278.50674	8
ATTAGTA	7245	0.0	271.99445	7
TAGTAGA	7255	0.0	271.41626	9
TATTAGT	7270	0.0	271.05914	6
GGGGAGC	6415	0.0	264.41934	3
GGGAGCG	5015	0.0	262.35297	4
GGAGCGA	5110	0.0	257.7642	5
CGAAAGC	5225	0.0	250.67943	9
GAGCGAA	5355	0.0	245.97105	6
GCGAAAG	5440	0.0	241.31433	8
AGCGAAA	5460	0.0	240.70055	7
GGGGGAG	7175	0.0	238.05574	2
AAACGTT	200	0.0	236.0	295
GGTCGGG	15	0.0028490007	196.66667	295
>>END_MODULE