##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_30427_flu.3510000003bb1b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350507
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.79512249398728	34.0	34.0	34.0	34.0	34.0
2	33.88550585295015	34.0	34.0	34.0	34.0	34.0
3	33.89239301925497	34.0	34.0	34.0	34.0	34.0
4	33.89249002159729	34.0	34.0	34.0	34.0	34.0
5	33.88025346141446	34.0	34.0	34.0	34.0	34.0
6	37.62382491647813	38.0	38.0	38.0	38.0	38.0
7	37.64909117364275	38.0	38.0	38.0	38.0	38.0
8	37.65006975609617	38.0	38.0	38.0	38.0	38.0
9	37.565338210078544	38.0	38.0	38.0	37.0	38.0
10-14	37.64930686120391	38.0	38.0	38.0	37.6	38.0
15-19	37.61323397250269	38.0	38.0	38.0	37.2	38.0
20-24	37.60770369778635	38.0	38.0	38.0	37.0	38.0
25-29	37.52306344809091	38.0	38.0	38.0	37.0	38.0
30-34	37.46126839121616	38.0	38.0	38.0	37.0	38.0
35-39	37.381362426428	38.0	38.0	38.0	37.0	38.0
40-44	37.397480221507706	38.0	38.0	38.0	37.0	38.0
45-49	37.369448256382896	38.0	38.0	38.0	37.0	38.0
50-54	37.32535612698177	38.0	38.0	38.0	37.0	38.0
55-59	37.26907479736495	38.0	38.0	38.0	37.0	38.0
60-64	37.10927313862491	38.0	38.0	38.0	36.6	38.0
65-69	37.030997954391786	38.0	38.0	38.0	36.2	38.0
70-74	37.11348189907762	38.0	38.0	38.0	36.4	38.0
75-79	37.03612823709655	38.0	38.0	38.0	36.0	38.0
80-84	36.918627017434744	38.0	38.0	38.0	35.8	38.0
85-89	36.94887691258663	38.0	38.0	38.0	36.0	38.0
90-94	36.79472250197571	38.0	38.0	38.0	35.4	38.0
95-99	36.788811065114245	38.0	38.0	38.0	35.2	38.0
100-104	36.75101838194387	38.0	38.0	38.0	35.2	38.0
105-109	36.59442521832659	38.0	38.0	38.0	34.6	38.0
110-114	36.49546514049648	38.0	37.0	38.0	34.0	38.0
115-119	36.57117718048427	38.0	37.0	38.0	34.0	38.0
120-124	36.47232038161862	38.0	37.0	38.0	34.0	38.0
125-129	36.38316838180121	38.0	37.0	38.0	34.0	38.0
130-134	36.21862330852166	38.0	37.0	38.0	33.6	38.0
135-139	35.94890886629939	38.0	37.0	38.0	31.8	38.0
140-144	35.91385050797844	38.0	37.0	38.0	31.6	38.0
145-149	35.4732470392888	38.0	36.2	38.0	29.2	38.0
150-154	35.07788831606787	38.0	35.6	38.0	27.4	38.0
155-159	35.14423449460354	38.0	35.6	38.0	27.6	38.0
160-164	35.07108902247316	38.0	35.8	38.0	27.8	38.0
165-169	34.82877317714054	38.0	35.2	38.0	26.0	38.0
170-174	34.74830516936895	38.0	35.0	38.0	25.6	38.0
175-179	34.358114388585676	37.8	34.6	38.0	23.6	38.0
180-184	34.078429246776814	37.2	34.2	38.0	23.0	38.0
185-189	33.30326584062515	37.0	33.2	37.8	22.0	38.0
190-194	32.33337993249778	36.8	30.2	37.0	20.2	38.0
195-199	32.04012016878407	36.6	29.8	37.2	20.2	37.8
200-204	31.87496740435997	36.8	29.8	37.0	19.8	37.4
205-209	30.802215077017006	35.0	27.0	37.0	17.2	37.2
210-214	30.07551689409912	34.2	25.4	37.0	15.4	37.0
215-219	29.276890333145985	33.0	24.0	36.8	14.2	37.0
220-224	27.824629465317383	31.2	21.4	36.2	9.6	37.0
225-229	26.85293988422485	29.4	20.0	36.0	9.2	37.0
230-234	26.46905596749851	28.8	19.8	35.6	9.0	37.0
235-239	25.374948859794525	27.4	18.4	34.0	9.0	37.0
240-244	24.438189822171886	25.8	16.8	33.0	8.4	37.0
245-249	23.619150544782272	25.0	15.8	32.2	8.0	36.2
250-254	22.808041494178433	23.6	14.4	31.6	8.0	36.0
255-259	22.29433192489736	22.6	13.6	31.0	8.0	36.0
260-264	21.088336609539894	21.2	12.0	29.2	7.8	35.2
265-269	20.347939984080206	19.8	11.4	28.6	7.4	34.0
270-274	19.46208777570776	19.0	9.4	27.2	7.6	33.4
275-279	18.238719340840554	18.0	8.2	25.2	7.0	32.0
280-284	18.11687184564074	17.4	8.2	25.2	7.0	31.4
285-289	16.969171514406277	15.6	8.0	23.2	7.0	30.2
290-294	16.27413375481802	14.6	8.0	22.0	7.0	29.2
295-299	15.439721888578546	13.0	8.0	21.0	7.0	27.0
300-301	13.504925721882874	11.0	8.0	17.0	7.0	23.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.12327041729709975
1101	2	0.024713433565061393
1101	3	0.037277064044857866
1101	4	-0.004357588998175288
1101	5	0.06974397301144108
1101	6	0.008757687217652688
1101	7	0.12216431451656007
1101	8	-0.026662137803640462
1101	9	0.03571184758552448
1101	10-14	0.035341269860353464
1101	15-19	0.008768557286664702
1101	20-24	0.008649281460606062
1101	25-29	-0.0010365467024584518
1101	30-34	0.029937625831372827
1101	35-39	0.09938850510839359
1101	40-44	0.1017077392733654
1101	45-49	0.05580164109650099
1101	50-54	0.06556646622216533
1101	55-59	0.013924847900909754
1101	60-64	0.22597713822732146
1101	65-69	0.20070211958200446
1101	70-74	0.14405931467000244
1101	75-79	0.05947516033689482
1101	80-84	0.14477233371174947
1101	85-89	0.08563792883739296
1101	90-94	0.22583462885604177
1101	95-99	0.07450588127218083
1101	100-104	0.19753527086864864
1101	105-109	0.04969925452012092
1101	110-114	0.07782958548763474
1101	115-119	0.09692081075363745
1101	120-124	0.1581226802172253
1101	125-129	0.18565407735123074
1101	130-134	0.2291920205805411
1101	135-139	0.10001704591965677
1101	140-144	0.17961741170001488
1101	145-149	0.5103539973414328
1101	150-154	0.5656710692535967
1101	155-159	0.20153429793716526
1101	160-164	0.34407640090337566
1101	165-169	0.2828546965147041
1101	170-174	0.3428666206935631
1101	175-179	0.28983584097507276
1101	180-184	-0.09165923206214899
1101	185-189	0.13089116787200084
1101	190-194	0.44420611473499605
1101	195-199	0.17684420530701317
1101	200-204	-0.07624441010311855
1101	205-209	-0.38723487290910796
1101	210-214	-0.19424947186205088
1101	215-219	-0.41402814959189627
1101	220-224	0.2990606665570503
1101	225-229	0.8727257223517348
1101	230-234	0.4878664987618997
1101	235-239	0.5498806140383152
1101	240-244	0.5662728337640672
1101	245-249	0.9664343575573824
1101	250-254	0.5524164602336761
1101	255-259	0.22752621769626558
1101	260-264	0.8820524529037925
1101	265-269	0.394031379908494
1101	270-274	0.41280675601122496
1101	275-279	0.577205148760612
1101	280-284	0.2902974576501549
1101	285-289	0.5333348836981635
1101	290-294	0.19527638414667337
1101	295-299	-0.023557061592967443
1101	300-301	0.18745618788078744
1102	1	0.06033475994087922
1102	2	0.06845607149445243
1102	3	0.07478692435564938
1102	4	0.031118305858193196
1102	5	0.06519850238720437
1102	6	0.08790547337890331
1102	7	0.1599996093000584
1102	8	0.05911460707341831
1102	9	0.1113046158655635
1102	10-14	0.07628375639196605
1102	15-19	0.0265026136505071
1102	20-24	0.06112346778598976
1102	25-29	-0.01162731639035286
1102	30-34	0.08755650669051818
1102	35-39	0.011267924561593645
1102	40-44	0.06757815268127132
1102	45-49	0.13492042114170744
1102	50-54	0.08383819693221994
1102	55-59	0.04785422154566987
1102	60-64	0.11927141942393149
1102	65-69	0.2077385788549435
1102	70-74	0.103889381277952
1102	75-79	-0.0893198477812831
1102	80-84	0.0609701271245342
1102	85-89	0.17510340271596903
1102	90-94	0.3010432429650436
1102	95-99	0.07471387634820559
1102	100-104	0.20073372576236892
1102	105-109	0.20483952738985067
1102	110-114	0.2022453068025385
1102	115-119	0.12096263615804048
1102	120-124	0.20439521743879396
1102	125-129	0.176178981931713
1102	130-134	0.25771422971462954
1102	135-139	0.1220864554329566
1102	140-144	0.17066584130181184
1102	145-149	0.3707786893283114
1102	150-154	0.7786318508622969
1102	155-159	0.5057454907606243
1102	160-164	0.40711730306432514
1102	165-169	0.6613286210775726
1102	170-174	0.39949929360716396
1102	175-179	0.5020198246467515
1102	180-184	0.6836318018643937
1102	185-189	0.7332880635101233
1102	190-194	0.8290910984102169
1102	195-199	0.5628632573729888
1102	200-204	0.21190234669474606
1102	205-209	0.095283282443404
1102	210-214	-0.04463309543341154
1102	215-219	0.11131576743646221
1102	220-224	0.7622204230471645
1102	225-229	1.3626998644604775
1102	230-234	1.1441496833504523
1102	235-239	1.0665849532825256
1102	240-244	1.117622502492999
1102	245-249	1.4222095813820133
1102	250-254	0.7504306024839202
1102	255-259	0.23180497354612584
1102	260-264	1.1034957547902522
1102	265-269	0.8963267541404498
1102	270-274	1.0727199499029574
1102	275-279	1.2068111961820733
1102	280-284	1.0517978520625846
1102	285-289	1.0442111160159264
1102	290-294	0.9845002583025746
1102	295-299	0.9191487845543502
1102	300-301	0.43714401840017203
1103	1	0.0557220800233722
1103	2	-0.03530800266216261
1103	3	-0.005595390403158262
1103	4	-0.025793816222183352
1103	5	0.01722521631261742
1103	6	0.03555297124766099
1103	7	0.08679453959695138
1103	8	0.03443110978478359
1103	9	0.1696882677355731
1103	10-14	0.09086109837053868
1103	15-19	0.07865065803693483
1103	20-24	0.06031058907046827
1103	25-29	0.12693772982487417
1103	30-34	0.11096656473812772
1103	35-39	-0.3445557607008283
1103	40-44	-0.1859664300728312
1103	45-49	-0.11568817669556353
1103	50-54	0.08528779526825048
1103	55-59	-0.25059819604335587
1103	60-64	0.2356234404781219
1103	65-69	0.25064852901394374
1103	70-74	0.14577421284792536
1103	75-79	0.2706219984933824
1103	80-84	0.2787487538618052
1103	85-89	0.28628101565411157
1103	90-94	0.36324084536193624
1103	95-99	0.35381992200100143
1103	100-104	0.3042876824442118
1103	105-109	0.13930268431646908
1103	110-114	0.4553215469024323
1103	115-119	0.1455810465521381
1103	120-124	0.33947316253234305
1103	125-129	0.34085236245304884
1103	130-134	0.44162503237046735
1103	135-139	0.537101719017187
1103	140-144	0.5268842927289512
1103	145-149	0.8535479951978004
1103	150-154	0.9135810370992559
1103	155-159	0.07570364413223274
1103	160-164	-0.6739300299648008
1103	165-169	0.6063273653249937
1103	170-174	0.846189235913279
1103	175-179	0.538067352229092
1103	180-184	0.5713632759764451
1103	185-189	0.6260680167466077
1103	190-194	0.2740024705763773
1103	195-199	0.6139288784045434
1103	200-204	0.3183965330908798
1103	205-209	0.8037619116568067
1103	210-214	0.7178619965195132
1103	215-219	0.6067649908153889
1103	220-224	1.2081710631272529
1103	225-229	1.8022005347847454
1103	230-234	1.0567839692870855
1103	235-239	1.4870724682719683
1103	240-244	1.2644507544500279
1103	245-249	1.308556544052557
1103	250-254	1.3777112083580079
1103	255-259	0.5387802369888739
1103	260-264	1.994163921285356
1103	265-269	0.7121449919127798
1103	270-274	1.0882622758397353
1103	275-279	1.7296167243233143
1103	280-284	0.9153778435022417
1103	285-289	0.6602373488642925
1103	290-294	0.9586204355936161
1103	295-299	1.2797655536267545
1103	300-301	-0.7369811111545825
2116	1	-0.012245583199813836
2116	2	0.004131234755455182
2116	3	0.03148472179496764
2116	4	0.07096452452261559
2116	5	0.055201545244685235
2116	6	-0.24800726683128715
2116	7	-0.1463084683162137
2116	8	0.11018194785005164
2116	9	0.13965702557462123
2116	10-14	0.15663040807840645
2116	15-19	0.1376940093858252
2116	20-24	-0.11448428447859982
2116	25-29	-0.1704075986739042
2116	30-34	0.06060123669543316
2116	35-39	-0.12864734356096363
2116	40-44	-0.12279728911823184
2116	45-49	0.10059628660059161
2116	50-54	0.1829218286996479
2116	55-59	0.15207677146023713
2116	60-64	0.36185340102633745
2116	65-69	0.27802601185356934
2116	70-74	-0.7490434651981843
2116	75-79	0.23689460345106994
2116	80-84	-0.13448150314487606
2116	85-89	0.17170757377137846
2116	90-94	0.2838686531656407
2116	95-99	0.28492594010777594
2116	100-104	0.27827322439308233
2116	105-109	0.3701443983024717
2116	110-114	0.5426408782745753
2116	115-119	0.3513366735617893
2116	120-124	-0.04934328407092892
2116	125-129	0.020799912109843888
2116	130-134	0.33394881061953186
2116	135-139	0.5070105010290007
2116	140-144	0.6683389915955686
2116	145-149	0.8812014124516878
2116	150-154	1.0137885580223838
2116	155-159	-0.2049681763507678
2116	160-164	-0.400335357095301
2116	165-169	0.7838640235539955
2116	170-174	1.0598190277082224
2116	175-179	0.7850341260768943
2116	180-184	0.798660943076392
2116	185-189	1.2338676108265574
2116	190-194	1.1904816842773158
2116	195-199	0.9079328919960261
2116	200-204	0.9416156158940119
2116	205-209	0.7334538229017014
2116	210-214	0.8623636042365526
2116	215-219	1.117897234093764
2116	220-224	1.7329841802739523
2116	225-229	1.4270827495574991
2116	230-234	1.4151479746689475
2116	235-239	1.5253420630360566
2116	240-244	2.1604998753366687
2116	245-249	1.426075414773866
2116	250-254	1.22440387215131
2116	255-259	1.026532169669995
2116	260-264	0.6154902308336041
2116	265-269	1.1671118113695798
2116	270-274	1.3429917004822727
2116	275-279	1.7194222019635
2116	280-284	0.4994983880736328
2116	285-289	-0.742237167110261
2116	290-294	0.5025001646761922
2116	295-299	0.6971327744427018
2116	300-301	1.2724622310725042
1117	1	-6.33551874010152E-4
1117	2	0.0691010871360831
1117	3	0.04710141964882553
1117	4	0.03215033088480368
1117	5	0.047413987779393096
1117	6	-0.3555499470817054
1117	7	-0.19358363176020532
1117	8	0.005352553343620059
1117	9	0.1392481268290382
1117	10-14	0.1159149507817645
1117	15-19	0.09080995643689249
1117	20-24	0.05626004984811317
1117	25-29	0.18027343720292066
1117	30-34	0.09311654152136128
1117	35-39	-0.027268176605524275
1117	40-44	0.05033760126456599
1117	45-49	0.21775547989188482
1117	50-54	0.26774290662142164
1117	55-59	0.36715058114905474
1117	60-64	0.2556212759079415
1117	65-69	0.43674779899519933
1117	70-74	-0.007577205120099961
1117	75-79	0.3227462448214595
1117	80-84	0.10211424153597193
1117	85-89	0.20155764423133604
1117	90-94	0.43982194168182076
1117	95-99	0.29458680826680705
1117	100-104	0.3942276679904637
1117	105-109	0.3727299320654964
1117	110-114	0.4079565619671399
1117	115-119	0.3972758700873129
1117	120-124	0.6389379551001824
1117	125-129	0.49075024361815167
1117	130-134	0.29820759501207306
1117	135-139	0.33620656451226694
1117	140-144	0.5084246986207717
1117	145-149	0.91932579159554
1117	150-154	1.076301552685905
1117	155-159	-0.009796877808483373
1117	160-164	-0.46107876735704423
1117	165-169	0.48124383735489573
1117	170-174	0.7595219408230207
1117	175-179	0.7833620305285791
1117	180-184	0.6618367233337352
1117	185-189	0.9240392558932058
1117	190-194	1.0668230800774339
1117	195-199	0.6059925865925564
1117	200-204	0.9565055366554382
1117	205-209	1.31526730038917
1117	210-214	0.7788239734005096
1117	215-219	0.5741030233607489
1117	220-224	1.0957013932940285
1117	225-229	0.9529572615356905
1117	230-234	1.3616783417537484
1117	235-239	0.5400457847076936
1117	240-244	0.784718462903367
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2108	185-189	-0.9974906759690327
2108	190-194	-0.7659409861431428
2108	195-199	-1.7097156057193956
2108	200-204	-0.795623503504153
2108	205-209	-0.5128811565601517
2108	210-214	-0.19325461702158364
2108	215-219	-0.9665085442788808
2108	220-224	-0.5480681112292523
2108	225-229	-0.15163927010373257
2108	230-234	-0.2548497176742508
2108	235-239	-0.6624328942722926
2108	240-244	-0.5114320949407194
2108	245-249	-0.3789155853645987
2108	250-254	-0.733414007573618
2108	255-259	-0.9293255863645307
2108	260-264	-0.10845678536615111
2108	265-269	-0.9134138728506649
2108	270-274	-1.3784408928624465
2108	275-279	-0.40666908893971865
2108	280-284	-0.8171301253338576
2108	285-289	-0.12436344209064387
2108	290-294	-0.6396002645479726
2108	295-299	0.04286176322748858
2108	300-301	-0.10104125348927084
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	10.0
14	22.0
15	77.0
16	167.0
17	392.0
18	978.0
19	1081.0
20	1085.0
21	1435.0
22	1994.0
23	3049.0
24	4330.0
25	6104.0
26	8382.0
27	11294.0
28	15033.0
29	20597.0
30	29135.0
31	41047.0
32	56127.0
33	66821.0
34	56458.0
35	22926.0
36	1960.0
37	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.60020484612291	20.83496192658064	15.192278613551228	31.37255461374523
2	46.454421737654314	22.659461865240925	16.36771876167951	14.518397635425256
3	46.52745879540209	21.151931345165718	17.977387042198874	14.343222817233322
4	46.84014869888476	20.937384988031624	17.7243250491431	14.49814126394052
5	30.43163189322895	20.837529635642085	34.433549115994836	14.297289355134135
6	28.69528996567829	21.671749779604973	34.724556142958626	14.908404111758111
7	16.731762846391085	48.931119777921694	19.51886838208652	14.8182489936007
8	28.190877785607704	20.237256317277545	35.94222084009734	15.629645057017406
9	15.403401358603395	18.801621650922804	37.42150656049665	28.37347042997715
10-14	26.928135529390286	34.9403007643214	22.43259050461189	15.698973201676427
15-19	21.861360828742367	35.87386271886153	20.71730379136508	21.54747266103102
20-24	25.48428419403892	30.746318903759413	26.31850433800181	17.45089256419986
25-29	19.529424519339127	25.55703595077987	37.482789216763145	17.430750313117855
30-34	19.600692710844577	27.819130573711792	31.063231262143127	21.516945453300504
35-39	20.597306187893537	30.010470546950565	27.44869574644729	21.943527518708613
40-44	20.61219890044992	28.912061670665633	29.010775819027867	21.46496360985658
45-49	22.06209861714602	29.808363313714136	25.525367539022042	22.604170530117802
50-54	22.45803935442088	29.812243407406985	26.722376443266466	21.007340794905666
55-59	21.967606923684833	27.214634800446213	28.958223373570284	21.85953490229867
60-64	22.036820947941123	25.889069262525428	28.929693272887558	23.144416516645887
65-69	19.989786223955587	28.17587095264859	27.913622267172983	23.920720556222843
70-74	20.91570211151275	26.494363878610127	29.10754992054367	23.48238408933345
75-79	20.18110907913394	25.579460609916495	30.02285261064686	24.216577700302704
80-84	24.111187508380716	25.383458818226167	27.023311945267853	23.482041728125257
85-89	21.108965013537535	28.258551184427127	25.966100534368785	24.666383267666554
90-94	20.253518474666695	30.573369433420734	28.68587503245299	20.48723705945958
95-99	22.345345456724118	30.36253198937539	26.23006102588536	21.062061528015132
100-104	22.830243047927716	28.499345234189335	25.912977486897553	22.757434230985403
105-109	23.655904161685786	27.619533989335448	23.629199987446757	25.095361861532012
110-114	21.4360340877643	28.81916766284268	28.93785288168282	20.806945367710203
115-119	24.503419332566825	27.565155617434172	26.24746438730182	21.68396066269718
120-124	19.222782997201197	28.29729506115427	27.618621026113598	24.861300915530933
125-129	20.79695983247125	24.272667878244942	31.645302376272085	23.28506991301172
130-134	25.538662565940196	28.731123772135792	25.424998644820217	20.305215017103794
135-139	24.678365909953296	27.430094120802153	24.454233438989807	23.437306530254745
140-144	20.956785456495876	29.342181468558405	26.775955972348626	22.925077102597093
145-149	20.841067368126744	27.162767077405015	29.06127409723629	22.934891457231952
150-154	23.32638149880031	25.333588202232765	25.89215051339916	25.44787978556776
155-159	22.427112725280807	26.044158889836723	29.446316336050348	22.08241204883212
160-164	25.25050854904467	29.63358791693176	25.645764563903146	19.470138970120427
165-169	21.00528662765651	25.613069068520744	28.61386505832979	24.767779245492957
170-174	22.751043488432472	29.13202874692945	26.02629904680934	22.090628717828746
175-179	20.275829013400585	29.27268214329528	28.563823261732292	21.887665581571838
180-184	20.183163246383096	30.01845897514172	26.74645584824269	23.051921930232492
185-189	21.67745579974152	27.65222948471785	28.13062221296579	22.539692502574844
190-194	19.677210440875644	27.38547304333437	27.47300339222897	25.464313123561013
195-199	20.69824568410902	30.443557475314332	27.82380951022376	21.03438733035289
200-204	22.469622575298068	27.310667119344263	27.332292935661766	22.887417369695896
205-209	21.08414382594356	27.47716878692865	28.3865942762912	23.052093110836587
210-214	23.681353011494778	27.129044498398038	27.70677903722322	21.482823452883963
215-219	23.190692339953266	26.681977820699732	29.14732088089539	20.980008958451617
220-224	18.825758116100392	27.01258462741115	29.382351850319683	24.779305406168778
225-229	21.643847341137267	28.49580750170467	26.964311697055983	22.896033460102082
230-234	21.588727186618243	26.63359076994183	29.314164909687968	22.463517133751964
235-239	22.310139312481635	26.574476401327217	26.329574017066705	24.78581026912444
240-244	23.16672705537978	30.927713283900175	23.737671430242475	22.167888230477566
245-249	20.980864861472096	29.46309203525179	24.82483944685841	24.7312036564177
250-254	21.55540402902082	25.750527093610113	26.78519972496983	25.908869152399237
255-259	22.39133597902467	26.48255241692748	28.07453203502355	23.051579569024298
260-264	21.428273900378596	27.770287041342968	27.637907374175125	23.163531684103315
265-269	21.128650783008613	27.31175126317021	27.945290678930807	23.614307274890372
270-274	19.495759000533514	30.017203650711682	25.857400850767604	24.6296364979872
275-279	21.669581491953085	27.813310433172518	23.932874379113684	26.584233695760712
280-284	18.049682317328898	29.10058857597708	27.897074808777	24.952654297917018
285-289	21.288932888644162	25.72947187930626	29.402494101401683	23.57910113064789
290-294	20.703209921627813	29.93321103430174	26.18486934640392	23.178709697666523
295-299	19.998630555167228	30.414228531812487	23.3861805898313	26.200960323188983
300-301	15.541772346914613	32.265118813604296	27.587466156168066	24.60564268331303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.25
29	0.75
30	1.25
31	2.0
32	2.75
33	4.25
34	16.0
35	41.25
36	144.5
37	709.25
38	2706.5
39	3772.75
40	6076.0
41	7592.0
42	11084.5
43	13614.75
44	16392.5
45	12874.5
46	8768.25
47	7651.5
48	7109.75
49	5960.0
50	5301.75
51	2662.0
52	689.0
53	177.25
54	111.0
55	138.5
56	170.5
57	225.5
58	329.25
59	667.5
60	849.0
61	505.0
62	203.75
63	70.75
64	23.25
65	13.5
66	6.75
67	2.5
68	0.5
69	0.25
70	0.5
71	0.25
72	0.5
73	0.25
74	0.25
75	0.25
76	0.0
77	0.0
78	0.25
79	0.0
80	0.0
81	0.0
82	0.25
83	0.25
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	350507.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.648219697669463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	36.724237232824755	3.5432350905162604
2	6.714966916253255	1.2957495214118684
3	5.4371199402320025	1.5737558311781343
4	4.897977725314235	1.8902706067249229
5	4.941798393037776	2.383977829880918
6	4.789941131515326	2.772864262545799
7	4.535588656926048	3.0632249074196056
8	4.226663873473525	3.2623825311580132
9	3.117973802921864	2.707460663595128
>10	23.24588898932155	40.75818401087773
>50	1.1382987203252666	7.416780929365297
>100	0.16201885188170384	2.4335202188161467
>500	0.008807708605132634	0.6730313646305348
>1k	0.0440385430256565	11.583730902861058
>5k	0.0058718057367542	3.904205092046119
>10k+	0.0088077086051313	10.737626236972519
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	16899	4.8213017143737495	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	10991	3.135743366038339	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	10017	2.8578601853885943	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	7424	2.118074674685527	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	6359	1.8142291024145023	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	4900	1.39797493345354	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	4855	1.3851363881463137	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4796	1.3683036287435058	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	3195	0.9115367168130736	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	3144	0.8969863654648836	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	3058	0.8724504788777401	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	3050	0.870168070823122	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	2845	0.8116813644235351	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2311	0.6593306267777819	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2267	0.6467773824773828	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	1469	0.41910717902923483	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1425	0.40655393472883566	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1374	0.3920035833806458	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT	1134	0.323531341742105	TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1071	0.3055573783119881	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	936	0.26704174239030887	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	829	0.23651453465979283	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	611	0.17431891517145165	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	499	0.14236520240679928	No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT	453	0.12924135609274565	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	438	0.12496184099033684	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.853010068272531E-4	0.0	0.0
2	2.853010068272531E-4	0.0	0.0
3	2.853010068272531E-4	0.0	0.0
4	2.853010068272531E-4	0.0	0.0
5	2.853010068272531E-4	0.0	0.0
6	5.706020136545062E-4	0.0	0.0
7	5.706020136545062E-4	0.0	0.0
8	8.559030204817593E-4	0.0	0.0
9	0.0011412040273090123	0.0	0.0
10-14	0.0023965284573489254	0.0	0.0
15-19	0.002681829464176179	0.0	0.0
20-24	0.003309491679196136	0.0	0.0
25-29	0.003594792686023389	0.0	0.0
30-34	0.007132525170681328	0.0	0.0
35-39	0.007589006781604933	0.0	0.0
40-44	0.007931367989797636	0.0	0.0
45-49	0.008958451614375747	0.0	0.0
50-54	0.01192558208537918	0.0	0.0
55-59	0.015463314570037117	0.0	0.0
60-64	0.018943986853329604	0.0	0.0
65-69	0.023794103969392907	0.0	0.0
70-74	0.031725471959190545	0.0	0.0
75-79	0.040056261358546334	0.0	0.0
80-84	0.05226714445075277	0.0	0.0
85-89	0.06824400083307894	0.0	0.0
90-94	0.08661738567275405	0.0	0.0
95-99	0.11052561004487785	0.0	0.0
100-104	0.14265050341362656	0.0	0.0
105-109	0.1803672965161894	0.0	0.0
110-114	0.22287714653345012	0.0	0.0
115-119	0.27582901340058824	0.0	0.0
120-124	0.3398505593326239	0.0	0.0
125-129	0.4113469916435335	0.0	0.0
130-134	0.4855252534186194	0.0	0.0
135-139	0.5774492378183603	0.0	0.0
140-144	0.6827253093376167	0.0	0.0
145-149	0.8072306687170299	0.0	0.0
150-154	0.9546742290453544	0.0	0.0
155-159	1.123401244482992	0.0	0.0
160-164	1.3161506046954838	0.0	0.0
165-169	1.5327511290787346	0.0	0.0
170-174	1.7809059448170792	0.0	0.0
175-179	2.0700299851358177	0.0	0.0
180-184	2.399267347014468	0.0	0.0
185-189	2.7788318064974455	0.0	0.0
190-194	3.1860134034413012	0.0	0.0
195-199	3.626232856975753	0.0	0.0
200-204	4.0963518560256995	0.0	0.0
205-209	4.6573106956494446	0.0	0.0
210-214	5.2401235923961575	0.0	0.0
215-219	5.837658021095156	0.0	0.0
220-224	6.5007546211630585	0.0	0.0
225-229	7.2165748472926365	0.0	0.0
230-234	8.014276462381636	0.0	0.0
235-239	8.832063268351273	0.0	0.0
240-244	9.668109338757855	0.0	0.0
245-249	10.472886418816172	0.0	0.0
250-254	11.358803105216158	0.0	0.0
255-259	12.239926734701445	0.0	0.0
260-264	13.176341699309859	0.0	0.0
265-269	14.043770880467438	0.0	0.0
270-274	14.905608161891204	0.0	0.0
275-279	15.701312669932411	0.0	0.0
280-284	16.46643291004174	0.0	0.0
285-289	17.1988005945673	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	5795	0.0	293.47284	1
GGGTTAT	5875	0.0	289.72766	2
GTTATTA	5930	0.0	286.79175	4
GGGGGGA	4260	0.0	285.6514	1
GGTTATT	5965	0.0	285.35623	3
TTATTAG	6045	0.0	281.33585	5
TTAGTAG	6085	0.0	279.72882	8
TAGTAGA	6360	0.0	267.63364	9
ATTAGTA	6360	0.0	267.40173	7
TATTAGT	6395	0.0	265.93823	6
GGAGCGA	3675	0.0	263.2925	5
GGGAGCG	3680	0.0	262.93478	4
GGGGAGC	4645	0.0	261.02258	3
CGAAAGC	3735	0.0	258.2731	9
GCGAAAG	3860	0.0	249.90932	8
GAGCGAA	3950	0.0	245.33543	6
AAACGTT	340	0.0	242.94118	295
AGCGAAA	4210	0.0	230.18408	7
GGGGGAG	5340	0.0	228.15544	2
GGGAGCA	1445	0.0	170.46713	4
>>END_MODULE