FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_30427_flu.3510000003bb1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_30427_flu.3510000003bb1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350507
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC168994.8213017143737495No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT109913.135743366038339No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG100172.8578601853885943No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA74242.118074674685527No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA63591.8142291024145023No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT49001.39797493345354No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG48551.3851363881463137No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA47961.3683036287435058No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA31950.9115367168130736No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG31440.8969863654648836No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT30580.8724504788777401No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG30500.870168070823122No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT28450.8116813644235351No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG23110.6593306267777819No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA22670.6467773824773828No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC14690.41910717902923483No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT14250.40655393472883566No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT13740.3920035833806458No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT11340.323531341742105TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG10710.3055573783119881No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT9360.26704174239030887No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA8290.23651453465979283No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA6110.17431891517145165No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC4990.14236520240679928No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT4530.12924135609274565No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4380.12496184099033684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA57950.0293.472841
GGGTTAT58750.0289.727662
GTTATTA59300.0286.791754
GGGGGGA42600.0285.65141
GGTTATT59650.0285.356233
TTATTAG60450.0281.335855
TTAGTAG60850.0279.728828
TAGTAGA63600.0267.633649
ATTAGTA63600.0267.401737
TATTAGT63950.0265.938236
GGAGCGA36750.0263.29255
GGGAGCG36800.0262.934784
GGGGAGC46450.0261.022583
CGAAAGC37350.0258.27319
GCGAAAG38600.0249.909328
GAGCGAA39500.0245.335436
AAACGTT3400.0242.94118295
AGCGAAA42100.0230.184087
GGGGGAG53400.0228.155442
GGGAGCA14450.0170.467134
Produced by FastQC (version 0.11.2)