##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_30337_flu.3510000003ba86.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426457
Sequences flagged as poor quality	0
Sequence length	301
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.82646550531472	34.0	34.0	34.0	34.0	34.0
2	33.88061398921814	34.0	34.0	34.0	34.0	34.0
3	33.894652919286116	34.0	34.0	34.0	34.0	34.0
4	33.89720651789043	34.0	34.0	34.0	34.0	34.0
5	33.89231974149797	34.0	34.0	34.0	34.0	34.0
6	37.6280609768394	38.0	38.0	38.0	38.0	38.0
7	37.64287606956856	38.0	38.0	38.0	38.0	38.0
8	37.65425353552644	38.0	38.0	38.0	38.0	38.0
9	37.59880363084672	38.0	38.0	38.0	37.0	38.0
10-14	37.6674909779884	38.0	38.0	38.0	38.0	38.0
15-19	37.620526805750636	38.0	38.0	38.0	37.2	38.0
20-24	37.62591070143063	38.0	38.0	38.0	37.4	38.0
25-29	37.54668301845204	38.0	38.0	38.0	37.0	38.0
30-34	37.504613126294096	38.0	38.0	38.0	37.0	38.0
35-39	37.4505907981344	38.0	38.0	38.0	37.0	38.0
40-44	37.43327603955381	38.0	38.0	38.0	37.0	38.0
45-49	37.429551396741054	38.0	38.0	38.0	37.0	38.0
50-54	37.382559554656154	38.0	38.0	38.0	37.0	38.0
55-59	37.35064262047521	38.0	38.0	38.0	37.0	38.0
60-64	37.182756526449324	38.0	38.0	38.0	36.6	38.0
65-69	37.13122589147323	38.0	38.0	38.0	36.8	38.0
70-74	37.18698016447145	38.0	38.0	38.0	37.0	38.0
75-79	37.09226299486232	38.0	38.0	38.0	37.0	38.0
80-84	36.97345007820249	38.0	38.0	38.0	36.0	38.0
85-89	37.0076964383279	38.0	38.0	38.0	36.0	38.0
90-94	36.88775984448608	38.0	38.0	38.0	35.8	38.0
95-99	36.9011089042975	38.0	38.0	38.0	36.0	38.0
100-104	36.88327639128916	38.0	38.0	38.0	35.8	38.0
105-109	36.75723226491768	38.0	38.0	38.0	35.6	38.0
110-114	36.66165826800826	38.0	38.0	38.0	35.0	38.0
115-119	36.73456831521115	38.0	38.0	38.0	35.0	38.0
120-124	36.587975341007414	38.0	37.6	38.0	34.8	38.0
125-129	36.510081438456865	38.0	37.0	38.0	34.0	38.0
130-134	36.42578829753059	38.0	37.0	38.0	34.0	38.0
135-139	36.25751435666433	38.0	37.0	38.0	34.0	38.0
140-144	36.17220446610092	38.0	37.0	38.0	33.6	38.0
145-149	35.73060871318796	38.0	37.0	38.0	30.4	38.0
150-154	35.45047777384355	38.0	36.6	38.0	29.0	38.0
155-159	35.503740353658166	38.0	36.4	38.0	29.6	38.0
160-164	35.46677953463069	38.0	36.0	38.0	29.4	38.0
165-169	35.211569278965996	38.0	35.6	38.0	28.0	38.0
170-174	35.11795937222276	38.0	35.4	38.0	28.0	38.0
175-179	34.81405909622775	38.0	35.2	38.0	26.4	38.0
180-184	34.45372827741132	37.6	35.0	38.0	24.0	38.0
185-189	33.77028727398073	37.0	34.0	37.8	23.0	38.0
190-194	32.85075306537353	36.8	31.8	37.0	21.2	38.0
195-199	32.63391807380346	36.8	31.2	37.2	20.8	37.8
200-204	32.49182121526907	37.0	30.8	37.0	20.4	37.4
205-209	31.584413903394715	35.6	28.6	37.0	19.4	37.2
210-214	30.879349148917708	35.0	27.2	37.0	17.0	37.0
215-219	30.136255237925514	34.6	25.8	37.0	15.2	37.0
220-224	28.837054615119463	33.0	23.2	36.4	11.0	37.0
225-229	28.001102573061292	31.6	22.0	36.2	9.8	37.0
230-234	27.633470197464227	31.2	21.4	36.0	9.0	37.0
235-239	26.675350152535895	30.0	19.8	35.8	9.0	37.0
240-244	25.723088142532543	27.8	18.6	34.8	9.0	37.0
245-249	24.886785772070805	27.0	17.2	34.0	8.6	37.0
250-254	23.937708608370833	25.2	15.4	33.2	8.0	36.0
255-259	23.36476549804552	24.6	14.8	32.6	8.0	36.0
260-264	22.368417448886994	22.8	13.4	31.4	8.0	36.0
265-269	21.457583296791938	21.6	12.2	30.4	8.0	36.0
270-274	20.515129075147087	20.2	10.2	29.0	8.0	35.0
275-279	19.207562778896815	18.8	9.0	26.8	7.0	33.8
280-284	18.7661222585161	18.2	9.0	26.2	7.0	32.8
285-289	17.54600674862882	16.4	8.2	24.2	7.0	31.2
290-294	17.142701843327696	15.6	8.0	23.6	7.0	30.6
295-299	16.13153963940092	14.0	8.0	22.0	7.0	29.2
300-301	14.311898972229322	12.0	8.0	18.5	7.0	25.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.07755365561167338
1101	2	0.025742937009397338
1101	3	0.0292944805528208
1101	4	0.027366506942904323
1101	5	0.01569820933173105
1101	6	0.11953913412135364
1101	7	0.11857407837883471
1101	8	0.026209397978725235
1101	9	0.09606845464569602
1101	10-14	0.05626858693766934
1101	15-19	0.08261974394097393
1101	20-24	0.035173442396498444
1101	25-29	0.06403115385861469
1101	30-34	0.056426203731376745
1101	35-39	0.11117852462172806
1101	40-44	0.057096456288292075
1101	45-49	0.07643607543060682
1101	50-54	0.055495847823664235
1101	55-59	0.11865234574488426
1101	60-64	0.2786739093219097
1101	65-69	0.22664444539367423
1101	70-74	0.19245761325922217
1101	75-79	0.10162151153996035
1101	80-84	0.08854068167259044
1101	85-89	0.059589419519419096
1101	90-94	0.24632386642009862
1101	95-99	0.09061283060410119
1101	100-104	0.13361509148627704
1101	105-109	0.08385018863270233
1101	110-114	0.14548901440576145
1101	115-119	0.25398992162793377
1101	120-124	0.23427090504279846
1101	125-129	0.21764880270496434
1101	130-134	0.25103450575598885
1101	135-139	0.168991216247953
1101	140-144	0.16951427304798727
1101	145-149	0.32366720571949514
1101	150-154	0.5206431583926516
1101	155-159	0.28141221213240186
1101	160-164	0.22843101541722177
1101	165-169	0.16701896149982076
1101	170-174	0.25694043570078406
1101	175-179	0.19115126156410156
1101	180-184	-0.05625320767762787
1101	185-189	0.15956801974479617
1101	190-194	0.1286510067578206
1101	195-199	-0.10651434401798099
1101	200-204	-0.33948450636108163
1101	205-209	-0.3901549255526078
1101	210-214	-0.5688515069349229
1101	215-219	-0.4638093881419181
1101	220-224	0.22084248052394528
1101	225-229	0.6221150641560698
1101	230-234	0.083605405242281
1101	235-239	0.1339086294386611
1101	240-244	0.6382589170761968
1101	245-249	0.535599304462945
1101	250-254	-0.12613369305863387
1101	255-259	-0.2830060506159171
1101	260-264	0.2781550425072439
1101	265-269	-0.0035473737118927318
1101	270-274	-0.006559856096330918
1101	275-279	0.2906059443014968
1101	280-284	-0.09174931363745742
1101	285-289	0.46696659648758754
1101	290-294	0.092396218615864
1101	295-299	0.03055888359957848
1101	300-301	0.4519655930771105
1102	1	-0.09704753313627634
1102	2	-0.004870653373252765
1102	3	0.035922948991554904
1102	4	-0.07588681396508434
1102	5	-0.06874155403663451
1102	6	0.014258605267613689
1102	7	0.09545856878195735
1102	8	0.07388195131427722
1102	9	0.017643981893684213
1102	10-14	0.039286149871315956
1102	15-19	0.023743994962053705
1102	20-24	0.016592860071703797
1102	25-29	-0.019081621515255165
1102	30-34	0.07503720226476673
1102	35-39	-0.0018426057448905908
1102	40-44	-0.08027559946720686
1102	45-49	-0.02611136293409544
1102	50-54	0.022673684762054336
1102	55-59	-0.07991404774215738
1102	60-64	0.08874782232669531
1102	65-69	0.12082111664703632
1102	70-74	0.04196921469687709
1102	75-79	-0.14094581061218747
1102	80-84	0.07528940738716017
1102	85-89	0.0637446295489994
1102	90-94	0.20358213643351775
1102	95-99	0.07588491410630382
1102	100-104	0.09423192572521799
1102	105-109	0.028428620790400316
1102	110-114	0.16335335973327147
1102	115-119	-0.017988418148618734
1102	120-124	0.0973623728918156
1102	125-129	0.06691044115169831
1102	130-134	0.14910251025047216
1102	135-139	0.07805290048078461
1102	140-144	0.13524038317893172
1102	145-149	0.36550162667496977
1102	150-154	0.5348365262030867
1102	155-159	0.2161691211013803
1102	160-164	0.28889795275578223
1102	165-169	0.42033485404969895
1102	170-174	0.45341814167149863
1102	175-179	0.4841292429794848
1102	180-184	0.42326107135711766
1102	185-189	0.48096315298963077
1102	190-194	0.5004148960848767
1102	195-199	0.5862939214249181
1102	200-204	0.06413858180628296
1102	205-209	0.1591896294202435
1102	210-214	-0.3368792180424265
1102	215-219	0.2157501916290343
1102	220-224	0.6819526883168052
1102	225-229	1.503685996615097
1102	230-234	0.5852065435850662
1102	235-239	1.0267803159122657
1102	240-244	1.0224305162649863
1102	245-249	1.3285990464105346
1102	250-254	0.840955483867436
1102	255-259	0.5472702110702556
1102	260-264	1.2723460320290236
1102	265-269	1.0850644256737958
1102	270-274	1.0235843863014402
1102	275-279	1.6392175980776784
1102	280-284	1.0106780777762765
1102	285-289	1.391301100359584
1102	290-294	1.194734464652253
1102	295-299	1.3183250153130857
1102	300-301	0.9368962222800707
1103	1	0.07661191162484471
1103	2	0.057475962965206406
1103	3	0.052931071439815014
1103	4	0.06238162121420032
1103	5	0.08227738450451483
1103	6	0.10608442369967719
1103	7	0.1266566259089359
1103	8	0.12541965994400073
1103	9	0.1748038553706266
1103	10-14	0.10364222190834482
1103	15-19	0.1091116127870535
1103	20-24	0.06370353900747716
1103	25-29	0.12998214936367702
1103	30-34	0.20719024981948309
1103	35-39	-0.2800913056262573
1103	40-44	-0.1920168243649769
1103	45-49	-0.05583486119129333
1103	50-54	0.13263186700699237
1103	55-59	-0.2000328746735036
1103	60-64	0.267790245233698
1103	65-69	0.3177249421972306
1103	70-74	0.22717923754574798
1103	75-79	0.27855940183766137
1103	80-84	0.2737490641909659
1103	85-89	0.29721400231461104
1103	90-94	0.3873670983281059
1103	95-99	0.34530797598348784
1103	100-104	0.35510142846276693
1103	105-109	0.14119924265985873
1103	110-114	0.3668795273651497
1103	115-119	0.1513281433748972
1103	120-124	0.41645643330162585
1103	125-129	0.35783332180263727
1103	130-134	0.508088218126538
1103	135-139	0.4419804163720116
1103	140-144	0.44711876520384664
1103	145-149	0.7968628672524005
1103	150-154	0.9296010837795379
1103	155-159	0.1241710179892408
1103	160-164	-0.42798387017260353
1103	165-169	0.4424870872838724
1103	170-174	0.7851196405003122
1103	175-179	0.6820354811538749
1103	180-184	0.783430897108488
1103	185-189	0.6939100513065455
1103	190-194	0.5854602984470532
1103	195-199	0.6035161622753904
1103	200-204	0.5943126707652127
1103	205-209	0.9201301856171469
1103	210-214	0.9711552516672484
1103	215-219	0.6655701629202468
1103	220-224	1.1320645376387617
1103	225-229	1.5695396201020166
1103	230-234	1.0626319813621876
1103	235-239	1.3848356226476604
1103	240-244	1.577138007534998
1103	245-249	1.636149713913884
1103	250-254	1.3065973840125729
1103	255-259	0.8845933957342069
1103	260-264	2.0055509901234494
1103	265-269	0.9989236823428413
1103	270-274	1.155293389652929
1103	275-279	2.1482322861849212
1103	280-284	0.939905479183615
1103	285-289	1.0482225854886593
1103	290-294	1.5714667719416973
1103	295-299	1.6174532133175248
1103	300-301	-0.9747001014618455
2116	1	0.007362347925024437
2116	2	0.03592389781105254
2116	3	0.016463357652732213
2116	4	0.03763234838115892
2116	5	0.009902748087995406
2116	6	-0.3732896309832796
2116	7	-0.1776970680992065
2116	8	0.014762301014386026
2116	9	0.019897514474756406
2116	10-14	0.08539207647495317
2116	15-19	0.051842968226260666
2116	20-24	-0.15846050985815197
2116	25-29	-0.16486834510985204
2116	30-34	0.07595488041867071
2116	35-39	-0.13454761743544452
2116	40-44	-0.10066929673264013
2116	45-49	0.08666985085059054
2116	50-54	0.14993215787377778
2116	55-59	0.1815903946691435
2116	60-64	0.3479619729823895
2116	65-69	0.31657217896278667
2116	70-74	-0.7921436245833959
2116	75-79	0.20189855250665545
2116	80-84	-0.1050055605908824
2116	85-89	0.13890754507202985
2116	90-94	0.19073789530309426
2116	95-99	0.18487749314462576
2116	100-104	0.14654901426848
2116	105-109	0.19314013852955014
2116	110-114	0.39643531561412715
2116	115-119	0.31267450754010895
2116	120-124	-0.26293251376062443
2116	125-129	-0.06247616045448723
2116	130-134	0.1308270197553938
2116	135-139	0.3915883279485115
2116	140-144	0.4544085848664423
2116	145-149	0.5151046357224516
2116	150-154	0.877028657950568
2116	155-159	-0.3044372426274009
2116	160-164	-0.4354440214233364
2116	165-169	0.6463837714025544
2116	170-174	0.7602316823851325
2116	175-179	0.6667945271108238
2116	180-184	0.47001239798107974
2116	185-189	1.0449881199510997
2116	190-194	0.8324364473708457
2116	195-199	0.8607052256843488
2116	200-204	0.7062642705325217
2116	205-209	0.9965164567049811
2116	210-214	0.9329981836044219
2116	215-219	1.0712772018964003
2116	220-224	1.3854076440960021
2116	225-229	1.0499733606488455
2116	230-234	1.314658043025947
2116	235-239	1.3521975772724382
2116	240-244	1.7775103170172528
2116	245-249	1.1019172531808934
2116	250-254	1.2902539285151953
2116	255-259	0.9671027616446217
2116	260-264	0.27769432927411586
2116	265-269	0.7470190866476685
2116	270-274	1.2616058969245998
2116	275-279	1.6077473530842816
2116	280-284	0.4189435245588342
2116	285-289	-0.9835647327196
2116	290-294	0.596853159376252
2116	295-299	0.702530118494952
2116	300-301	1.5507065303008059
1117	1	-0.0030014497679715646
1117	2	0.02541970772922042
1117	3	0.028149840941701143
1117	4	0.03153632156463715
1117	5	0.0287003424880794
1117	6	-0.38086451021032985
1117	7	-0.15282789724246015
1117	8	-0.01305589872927726
1117	9	1.2506056565797508E-4
1117	10-14	0.1099591670793032
1117	15-19	0.11277421144819044
1117	20-24	0.06058710918947696
1117	25-29	0.13116399246285937
1117	30-34	0.11698211102105915
1117	35-39	-0.08896457494309118
1117	40-44	-0.04215611522815266
1117	45-49	0.14074426670389073
1117	50-54	0.19849546261595918
1117	55-59	0.2841094915832727
1117	60-64	0.28793097623586306
1117	65-69	0.33174275965883027
1117	70-74	-0.09543140926248128
1117	75-79	0.2022495334787493
1117	80-84	0.13282372068684367
1117	85-89	0.16401585622257642
1117	90-94	0.36733290664096785
1117	95-99	0.30820984057634604
1117	100-104	0.29838921933600204
1117	105-109	0.2931085108060003
1117	110-114	0.40553905218121855
1117	115-119	0.42844727693772455
1117	120-124	0.38774393304998966
1117	125-129	0.38018025283546564
1117	130-134	0.27266513471152365
1117	135-139	0.35900704036100706
1117	140-144	0.39542737615107626
1117	145-149	0.7496163975286265
1117	150-154	0.8463063864332696
1117	155-159	-0.09769493758944492
1117	160-164	-0.5954432327020456
1117	165-169	0.3437036176019035
1117	170-174	0.5829993842484811
1117	175-179	0.7498609177250444
1117	180-184	0.6809540128879163
1117	185-189	0.9141362615265791
1117	190-194	0.9662485345245457
1117	195-199	0.8766397618176356
1117	200-204	0.8930901011546197
1117	205-209	1.1527053554528948
1117	210-214	0.796295432938944
1117	215-219	0.42549697908263084
1117	220-224	1.06729962871594
1117	225-229	0.632076959189714
1117	230-234	1.2022296096236005
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2108	185-189	-0.44355774187861385
2108	190-194	-0.12047385097388741
2108	195-199	-1.0578643054882129
2108	200-204	-0.7421794602634009
2108	205-209	-0.15464302391747964
2108	210-214	0.3413193254420648
2108	215-219	-0.4800264357287425
2108	220-224	0.029667650043077032
2108	225-229	0.1774831634043217
2108	230-234	0.28755161027741494
2108	235-239	-0.10329763533535541
2108	240-244	0.029126929169095206
2108	245-249	0.049348475304004324
2108	250-254	-0.3967312632778466
2108	255-259	-0.5407336542538843
2108	260-264	0.06709601427758827
2108	265-269	-0.6236674035892236
2108	270-274	-0.5491609977131269
2108	275-279	0.22445085194442882
2108	280-284	-0.2786517177843173
2108	285-289	-0.02980376532937612
2108	290-294	-0.32182611508107684
2108	295-299	0.2177245678952886
2108	300-301	-0.1678923397556904
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	4.0
14	33.0
15	123.0
16	206.0
17	481.0
18	1215.0
19	1162.0
20	989.0
21	1416.0
22	1940.0
23	2789.0
24	4173.0
25	5995.0
26	8445.0
27	11308.0
28	15611.0
29	22456.0
30	31167.0
31	42828.0
32	60422.0
33	78850.0
34	82377.0
35	46292.0
36	6170.0
37	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.123921051829377	22.851307400277168	18.232318850435096	29.79245269745836
2	27.22595713049616	25.673397317900747	18.92805136273997	28.17259418886312
3	40.06828355496568	25.131490396452634	21.14374954567518	13.656476502906505
4	41.03156941966013	24.601307986502743	20.72494999495846	13.642172598878668
5	39.90085753077098	24.63460559915771	21.424199860712804	14.040337009358506
6	24.94155331018133	25.608209033970596	34.79131541984303	14.658922236005036
7	17.20454817250039	32.84363956975733	35.57568523907451	14.376127018667766
8	24.265283486963515	35.81275486156869	23.666629929863973	16.25533172160382
9	14.9311653929939	22.048412852878485	38.361429171053594	24.658992583074028
10-14	22.53713739017064	33.77320573938287	27.914608037856105	15.775048832590391
15-19	22.915229436965507	32.41902466133749	24.1734102148634	20.492335686833606
20-24	21.4288427672661	29.90303828990965	28.945239496596376	19.72287944622787
25-29	17.490110374551243	28.305456353161045	34.38048853694511	19.823944735342604
30-34	17.305050685063208	30.291541702914948	31.920920514846753	20.482487097175095
35-39	18.877260778929646	31.606984994970183	31.109584319169343	18.406169906930828
40-44	18.472905826378742	29.819700462180244	32.33892279878159	19.368470912659426
45-49	19.799745343610258	28.714735600541204	33.08844736984034	18.3970716860082
50-54	18.869991581800743	31.85690468206642	31.43871480594761	17.834388930185224
55-59	20.4751710020002	31.82712442286092	28.108156273668854	19.58954830147002
60-64	18.293614596547837	31.074035600306715	29.658418082010613	20.97393172113484
65-69	18.54250252663223	31.723667333400552	29.73692541100275	19.996904728964466
70-74	19.662896845402937	32.073292266277726	26.91352234809137	21.35028854022797
75-79	18.73215822462757	34.3553980823389	27.827424570355277	19.085019122678254
80-84	19.996857830918476	31.608298140257986	26.655301706854384	21.739542321969154
85-89	19.88158243386789	32.06517890432095	26.85996477956746	21.193273882243695
90-94	19.206907144213837	30.499675231031496	29.850324886213613	20.44309273854105
95-99	20.100783900838774	29.777679812970593	28.382744332957365	21.738791953233267
100-104	19.44824448889337	28.429267194582337	30.45587245607412	21.666615860450175
105-109	19.591752509631686	28.340864377885694	32.53172066585846	19.535662446624162
110-114	18.78365227912779	28.68636228271549	29.96123876498686	22.568746673169862
115-119	17.609887984017146	30.401376926630352	31.04903894179249	20.93969614756001
120-124	20.72143264150899	29.559041122551626	28.5711337837109	21.148392452228478
125-129	20.937163653076393	30.453480655728477	29.386409415251713	19.22294627594341
130-134	20.46208644716818	29.616209840616992	29.9728694803931	19.94883423182173
135-139	19.899591283529173	27.46190119988651	32.20123013574639	20.43727738083793
140-144	18.887578349048088	30.29998335119367	29.90819707496887	20.90424122478937
145-149	18.816715401552795	31.58789749025107	28.76153985044213	20.833847257754005
150-154	19.206391265707914	28.33251652569896	29.60603296463653	22.855059243956603
155-159	18.828158524775066	30.510508679655864	31.210977894606025	19.450354900963053
160-164	20.155279430282537	29.057747908933372	29.06989450284554	21.71707815793855
165-169	20.039488154726033	28.93149836912045	29.61377114222536	21.415242333928155
170-174	18.476845262242147	30.682765202587838	32.14790705745245	18.692482477717565
175-179	18.95750333562352	31.01503785844763	29.598154092909716	20.429304713019132
180-184	21.837934422462286	28.83737399081267	28.86635698323629	20.458334603488744
185-189	18.77338160705534	30.861306063682857	28.82011550988728	21.54519681937452
190-194	19.879378225706226	29.471623164820837	28.74235854963103	21.906640059841905
195-199	19.289869787575302	31.18734127942559	31.37286994937356	18.149918983625547
200-204	20.39919616749168	28.27445674475973	29.580567325662376	21.74577976208621
205-209	21.22314793754118	28.266202688664976	30.131525569987126	20.379123803806714
210-214	20.60615724445841	27.98669033454721	29.62568324590756	21.78146917508682
215-219	19.739059272095428	30.229683180250294	28.555094651981328	21.47616289567295
220-224	20.578252907092626	28.942941492342722	29.983327744649518	20.495477855915134
225-229	18.915576482505855	31.055041891679586	29.265177966360035	20.764203659454527
230-234	17.653503166790554	30.33051397913506	27.72298262192906	24.293000232145328
235-239	19.9285273779068	29.724262938584662	27.66797121397937	22.679238469529167
240-244	17.856618603985865	30.117549952281237	29.3547063361605	22.671125107572394
245-249	20.468145894261742	29.207694659810794	28.34219081510718	21.981968630820283
250-254	20.63917346883742	30.506709937930438	26.979038918343466	21.875077674888676
255-259	19.289025622747427	30.17101372471316	26.921307423726194	23.618653228813223
260-264	17.62545813528679	32.06494441409099	27.5546186368145	22.75497881380772
265-269	19.27973980964083	31.356314939138063	26.785537580576708	22.578407670644403
270-274	18.10780453832391	30.492875014362525	28.234452711527773	23.164867735785787
275-279	18.55413324203847	31.94882485221253	25.30346553110865	24.19357637464035
280-284	18.719448854163492	30.501222866549266	27.371997645718093	23.407330633569153
285-289	19.19490124443965	31.84729058263788	25.874543037164354	23.08326513575812
290-294	17.097245443268605	33.23880250529362	25.176887704973772	24.487064346464006
295-299	18.67611505966604	31.035344712362562	25.400685180451955	24.887855047519444
300-301	13.522230846251789	33.5631728404036	34.144122385140825	18.77047392820378
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.25
21	0.25
22	0.0
23	0.0
24	0.25
25	0.0
26	0.25
27	0.0
28	1.5
29	3.0
30	10.5
31	60.25
32	321.75
33	1041.25
34	2692.5
35	5187.5
36	8245.0
37	9060.5
38	10729.5
39	11723.0
40	14490.75
41	16422.5
42	14461.0
43	12539.25
44	12129.5
45	10115.5
46	6320.0
47	3541.0
48	1932.25
49	739.0
50	227.25
51	89.5
52	39.75
53	24.75
54	16.5
55	11.5
56	8.0
57	9.0
58	8.75
59	11.0
60	9.0
61	6.0
62	7.25
63	9.0
64	3.5
65	2.0
66	0.25
67	0.5
68	0.5
69	0.25
70	0.0
71	0.0
72	0.25
73	0.0
74	0.25
75	0.0
76	0.25
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.25
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	4.6898045992913705E-5
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	426457.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.301574216630467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	39.231959194719984	4.04150939308226
2	5.180515642300986	1.0673493273915733
3	4.71937437183168	1.4585095604246354
4	3.9673566970575336	1.6348007783433638
5	3.238589843714438	1.6681286816124976
6	3.725397147891439	2.302647312325628
7	3.7115338179370223	2.6764248758108447
8	3.4740261613671684	2.8630350665471793
9	3.2568048556325677	3.019519523644323
>10	28.809838009608857	53.89633319560999
>50	0.48075378698699295	3.147016618431457
>100	0.11623453782242346	2.6251567474771944
>500	0.011528412402931076	0.8515149037666829
>1k	0.07147615583345529	15.453154541257499
>5k	0.004611364892480985	3.2948994742748843
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT	8373	1.9633866954933323	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG	5499	1.2894617745751622	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT	4140	0.9707895520533136	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT	4007	0.9396023514680261	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA	3838	0.8999735026040141	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA	3426	0.8033635278586119	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA	3328	0.780383485322084	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT	2974	0.6973739439146267	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT	2743	0.6432067007928114	No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAACATAAATCCGTATTTTCT	2707	0.634765052514087	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG	2522	0.5913843599706419	No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAACATAAATCCGTATTTTCT	2474	0.5801288289323425	No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	2213	0.5189268789115902	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG	2205	0.5170509570718737	No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAACATAAATCCGTATTTTCT	2121	0.4973537777548499	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC	1981	0.4645251455598102	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1807	0.4237238455459753	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA	1797	0.4213789432463297	No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	1790	0.4197375116365777	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT	1729	0.4054336076087389	TruSeq Adapter, Index 6 (97% over 36bp)
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT	1677	0.39324011565058137	No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	1632	0.38268805530217587	No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA	1563	0.3665082294346206	No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1479	0.34681105011759683	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1417	0.3322726558597936	No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA	1362	0.31937569321174236	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG	1358	0.31843773229188405	No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT	1241	0.2910023753860295	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTGACAAGA	1153	0.2703672351491475	No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	1140	0.2673188621596081	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG	1130	0.2649739598599624	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA	1060	0.24855964376244263	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT	1046	0.24527678054293867	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA	920	0.21573101156740307	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	788	0.18477830121208	No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTTCATTTTAATCATTTGTT	679	0.15921886614594202	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA	672	0.15757743453619005	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT	526	0.12334186096136304	No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	496	0.11630715406242599	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG	493	0.11560368337253228	No Hit
GGGGGGAGCAGAAGCAGAGGATCTTCTCAAAAAACTGAGGCAAATAGGCC	481	0.11278980061295744	No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAGAAACAATCAAGTTCAG	436	0.10223774026455187	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	7.034706898937056E-4	0.0	0.0
2	7.034706898937056E-4	0.0	0.0
3	7.034706898937056E-4	0.0	0.0
4	7.034706898937056E-4	0.0	0.0
5	7.034706898937056E-4	0.0	0.0
6	9.379609198582741E-4	0.0	0.0
7	0.0011724511498228426	0.0	0.0
8	0.0016414316097519796	0.0	0.0
9	0.0021104120696811167	0.0	0.0
10-14	0.0029076788515606496	0.0	0.0
15-19	0.004033231955390579	0.0	0.0
20-24	0.005580867473156731	0.0	0.0
25-29	0.006471930347022091	0.0	0.0
30-34	0.01097414276234181	0.0	0.0
35-39	0.012334186096136304	0.0	0.0
40-44	0.05876325162912088	0.0	0.0
45-49	0.13534776073554894	0.0	0.0
50-54	0.14060034188675527	0.0	0.0
55-59	0.150542727637253	0.0	0.0
60-64	0.17127166396612087	0.0	0.0
65-69	0.1987539189179683	0.0	0.0
70-74	0.22754931915761728	0.0	0.0
75-79	0.2654429403198916	0.0	0.0
80-84	0.31027747228911706	0.0	0.0
85-89	0.3674461903544789	0.0	0.0
90-94	0.44449967992083617	0.0	0.0
95-99	0.5322928220195706	0.0	0.0
100-104	0.6294655733168877	0.0	0.0
105-109	0.7370496908246318	0.0	0.0
110-114	0.8623143716717043	0.0	0.0
115-119	0.985796926771046	0.0	0.0
120-124	1.1234895898062407	0.0	0.0
125-129	1.2661065476706912	0.0	0.0
130-134	1.4270137434723782	0.0	0.0
135-139	1.6083684873269752	0.0	0.0
140-144	1.8662608422420077	0.0	0.0
145-149	2.231409028342834	0.0	0.0
150-154	2.570528798917593	0.0	0.0
155-159	3.6791986061900728	0.0	0.0
160-164	3.9896636706631616	0.0	0.0
165-169	4.37624426378275	0.0	0.0
170-174	4.778301212079998	0.0	0.0
175-179	5.250658331320625	0.0	0.0
180-184	5.782106988512323	0.0	0.0
185-189	6.4056165099881115	0.0	0.0
190-194	7.089436918610787	0.0	0.0
195-199	7.879575197499396	0.0	0.0
200-204	8.750002931127876	0.0	0.0
205-209	9.683555434662814	0.0	0.0
210-214	10.722675439727803	0.0	0.0
215-219	11.744818352143358	0.0	0.0
220-224	12.828772889177571	0.0	0.0
225-229	13.936692327714166	0.0	0.0
230-234	15.160684430083219	0.0	0.0
235-239	16.3818626496927	0.0	0.0
240-244	17.62044004436555	0.0	0.0
245-249	18.900428413650147	0.0	0.0
250-254	20.253249448361732	0.0	0.0
255-259	21.498767753841538	0.0	0.0
260-264	22.772284192779107	0.0	0.0
265-269	23.899009747758857	0.0	0.0
270-274	24.962985717200095	0.0	0.0
275-279	26.041875265267073	0.0	0.0
280-284	27.07433574780107	0.0	0.0
285-289	28.01393809926909	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGCTT	10	8.4631634E-4	295.0	295
CCGGGTT	5660	0.0	290.30917	1
CGGGTTA	5770	0.0	284.26343	2
TTATTAG	6465	0.0	253.93271	6
GTTATTA	6480	0.0	252.88965	5
GGGGGGA	4455	0.0	252.28957	1
TATTAGT	6705	0.0	244.84341	7
ATTAGTA	6725	0.0	244.11525	8
GGGTTAT	6955	0.0	236.25449	3
GGGGAGC	4890	0.0	229.24336	3
GAGCAGA	4915	0.0	226.8769	6
GGAGCAG	4990	0.0	223.46693	5
CAGAAGC	5065	0.0	220.44916	9
TTAGTAG	7480	0.0	219.47527	9
GGGAGCA	5155	0.0	217.17265	4
GGTTATT	7830	0.0	210.41826	4
GGGGGAG	5355	0.0	209.61252	2
GCAGAAG	5860	0.0	190.79352	8
GCTCGCA	35	1.0058411E-6	168.57143	295
AGCAGAA	6875	0.0	162.62546	7
>>END_MODULE