##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_30304_flu.3510000003baa0.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	384180
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.79779009839138	34.0	34.0	34.0	34.0	34.0
2	33.879551252017286	34.0	34.0	34.0	34.0	34.0
3	33.88642823676402	34.0	34.0	34.0	34.0	34.0
4	33.88784423967932	34.0	34.0	34.0	34.0	34.0
5	33.88957259617887	34.0	34.0	34.0	34.0	34.0
6	37.647147170597115	38.0	38.0	38.0	38.0	38.0
7	37.636711957936384	38.0	38.0	38.0	38.0	38.0
8	37.651009943255765	38.0	38.0	38.0	38.0	38.0
9	37.572039148315895	38.0	38.0	38.0	37.0	38.0
10-14	37.65218022801811	38.0	38.0	38.0	38.0	38.0
15-19	37.62307512103701	38.0	38.0	38.0	37.4	38.0
20-24	37.611465458899474	38.0	38.0	38.0	37.0	38.0
25-29	37.52146858243532	38.0	38.0	38.0	37.0	38.0
30-34	37.460020823572286	38.0	38.0	38.0	37.0	38.0
35-39	37.38514394294341	38.0	38.0	38.0	37.0	38.0
40-44	37.376514133999684	38.0	38.0	38.0	37.0	38.0
45-49	37.34327815086679	38.0	38.0	38.0	37.0	38.0
50-54	37.28620073923681	38.0	38.0	38.0	37.0	38.0
55-59	37.25045603623302	38.0	38.0	38.0	37.0	38.0
60-64	37.06304544744651	38.0	38.0	38.0	36.4	38.0
65-69	37.03607631839242	38.0	38.0	38.0	36.4	38.0
70-74	37.112895517726066	38.0	38.0	38.0	37.0	38.0
75-79	36.99764225102816	38.0	38.0	38.0	36.2	38.0
80-84	36.87908584517673	38.0	38.0	38.0	35.8	38.0
85-89	36.921978759956275	38.0	38.0	38.0	36.0	38.0
90-94	36.775750950075484	38.0	38.0	38.0	35.4	38.0
95-99	36.75922432193242	38.0	38.0	38.0	35.2	38.0
100-104	36.749660055182474	38.0	38.0	38.0	35.4	38.0
105-109	36.56434744130355	38.0	38.0	38.0	34.6	38.0
110-114	36.46413295850903	38.0	37.0	38.0	34.0	38.0
115-119	36.536301212973086	38.0	37.0	38.0	34.0	38.0
120-124	36.43291790306627	38.0	37.0	38.0	34.0	38.0
125-129	36.38496173668593	38.0	37.0	38.0	34.0	38.0
130-134	36.24204956010203	38.0	37.0	38.0	33.8	38.0
135-139	35.994449476807745	38.0	37.0	38.0	32.2	38.0
140-144	35.92358425737935	38.0	37.0	38.0	31.8	38.0
145-149	35.485371440470615	38.0	36.2	38.0	29.2	38.0
150-154	35.15689208183664	38.0	36.0	38.0	27.8	38.0
155-159	35.17523036076839	38.0	35.8	38.0	28.0	38.0
160-164	35.107114894060075	38.0	35.8	38.0	27.8	38.0
165-169	34.83320839189963	38.0	35.2	38.0	26.0	38.0
170-174	34.710057785413085	38.0	35.0	38.0	25.4	38.0
175-179	34.40662866364725	38.0	34.6	38.0	23.6	38.0
180-184	34.08470716851476	37.6	34.6	38.0	23.0	38.0
185-189	33.29008537664636	37.0	33.4	37.8	21.6	38.0
190-194	32.31588994742047	36.8	30.2	37.0	20.2	38.0
195-199	32.08193971575824	36.8	29.8	37.2	20.0	37.8
200-204	31.947328335675984	36.8	29.8	37.0	19.8	37.4
205-209	30.776941017231508	35.0	27.0	37.0	17.0	37.2
210-214	29.97799156645322	34.0	25.2	37.0	15.4	37.0
215-219	29.282327034202716	33.0	23.8	37.0	13.8	37.0
220-224	27.846234577541775	31.2	21.8	36.2	9.4	37.0
225-229	26.7313061585715	29.4	20.0	35.8	9.0	37.0
230-234	26.474944557238796	29.0	19.8	35.8	9.0	37.0
235-239	25.540115570826178	27.6	18.4	34.6	9.0	37.0
240-244	24.608124837315838	26.4	16.8	33.6	8.2	37.0
245-249	23.657671924618672	25.0	16.0	32.4	8.0	36.2
250-254	22.879907335103333	23.8	14.2	31.8	8.0	36.0
255-259	22.267133114685826	22.8	13.6	31.0	8.0	36.0
260-264	21.28972721120308	21.2	12.4	29.6	8.0	35.8
265-269	20.567491800718415	20.6	11.6	28.8	7.6	34.6
270-274	19.437167473580093	19.0	9.2	27.2	7.0	33.8
275-279	18.432540996407933	18.0	8.6	25.8	7.0	32.2
280-284	18.338555885262117	18.0	8.4	25.6	7.0	32.0
285-289	17.027471497735434	15.8	8.0	23.4	7.0	30.2
290-294	16.34390077567807	14.8	8.0	22.2	7.0	29.4
295-299	15.61933728981207	13.0	8.0	21.0	7.0	27.0
300-301	13.792789838096725	12.0	8.0	18.0	7.0	24.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.10878266749595156
1101	2	0.03345253944477378
1101	3	0.024881610258745468
1101	4	0.03229325411264483
1101	5	0.06843347507559372
1101	6	0.13159287380987905
1101	7	0.07293019270842649
1101	8	0.07540761684683162
1101	9	0.07472158905076753
1101	10-14	0.06262763944509686
1101	15-19	0.004431385925649067
1101	20-24	0.03256541292179804
1101	25-29	0.08718650491237412
1101	30-34	0.06619730532961654
1101	35-39	0.0596082289316584
1101	40-44	-0.04545583149005239
1101	45-49	0.08487281483284193
1101	50-54	0.018419652853367552
1101	55-59	0.029860207965406005
1101	60-64	0.17623392572862429
1101	65-69	0.1662464766762568
1101	70-74	0.06484171277877948
1101	75-79	0.03189621742392035
1101	80-84	0.11052269511507973
1101	85-89	0.1192717606029916
1101	90-94	0.25569336545309795
1101	95-99	0.007910929959010105
1101	100-104	0.11335698427288321
1101	105-109	-0.0023114727014785785
1101	110-114	0.037956634404260114
1101	115-119	0.07438844726058136
1101	120-124	0.029812737531315747
1101	125-129	0.13130891396235
1101	130-134	0.01635423303638106
1101	135-139	0.043486598031783785
1101	140-144	-0.059174418384046135
1101	145-149	0.10082842726129826
1101	150-154	0.5096474136004616
1101	155-159	0.217545741017922
1101	160-164	0.2091177578777348
1101	165-169	0.10159123650198154
1101	170-174	0.21357340432225413
1101	175-179	0.21708065717145786
1101	180-184	-0.19364225974170068
1101	185-189	-0.02180599963948282
1101	190-194	0.22433886462247443
1101	195-199	-0.11964124693454181
1101	200-204	-0.24913293344697252
1101	205-209	-0.2243546704784869
1101	210-214	-0.5158783885092006
1101	215-219	-0.46712248130181067
1101	220-224	0.22970000622822084
1101	225-229	0.5971884784917414
1101	230-234	0.4667288094372104
1101	235-239	0.38731410969076663
1101	240-244	0.40159205495308825
1101	245-249	0.4310167523682189
1101	250-254	0.20515494871686002
1101	255-259	-0.21657243520815328
1101	260-264	0.4254714339487258
1101	265-269	0.2931504343425786
1101	270-274	0.23614958332391467
1101	275-279	0.49783979469457407
1101	280-284	0.2349604914070511
1101	285-289	0.3177465623643485
1101	290-294	0.07939162612778006
1101	295-299	0.30600708656768383
1101	300-301	0.2337074553851366
1102	1	-0.05186253128515972
1102	2	-0.06056684752536512
1102	3	0.007562597146780092
1102	4	0.03012875854681596
1102	5	0.023966809831598823
1102	6	0.08574316703297313
1102	7	0.18232867634209526
1102	8	0.03449153728688259
1102	9	0.10731807931612991
1102	10-14	0.067501514390095
1102	15-19	0.045850075582713146
1102	20-24	-0.005485199691413811
1102	25-29	0.012128708815986045
1102	30-34	0.05655910127154584
1102	35-39	-0.0167915544155548
1102	40-44	-0.007264780713832408
1102	45-49	0.09321150859532423
1102	50-54	-0.03406987271562656
1102	55-59	-0.014197337130788412
1102	60-64	0.04388955346790624
1102	65-69	0.18764510664201595
1102	70-74	0.07789392589927502
1102	75-79	-0.10330569546174218
1102	80-84	0.00470223443318929
1102	85-89	0.04422412645762108
1102	90-94	0.19600694293746557
1102	95-99	-0.026867331188903165
1102	100-104	0.00808201712139578
1102	105-109	0.12110745474524265
1102	110-114	0.1583806316483347
1102	115-119	-0.01503123420290109
1102	120-124	0.07690829950244904
1102	125-129	-0.0430561419334623
1102	130-134	7.243394218363619E-4
1102	135-139	0.09639377978741948
1102	140-144	0.20510764485783994
1102	145-149	0.4282731132214508
1102	150-154	0.7605478132699872
1102	155-159	0.5256477629413183
1102	160-164	0.4714235248865535
1102	165-169	0.5490426699976041
1102	170-174	0.32316150996182813
1102	175-179	0.23444174076061586
1102	180-184	0.2815610554931922
1102	185-189	0.7870029892159067
1102	190-194	0.7916960358604896
1102	195-199	0.6703396790834049
1102	200-204	0.4085595110408349
1102	205-209	0.21729286631540035
1102	210-214	-0.19895193156488133
1102	215-219	0.12845097283679152
1102	220-224	0.9892353205322877
1102	225-229	1.2925516818638876
1102	230-234	0.6871396977644793
1102	235-239	1.238988240310448
1102	240-244	1.4467932260163288
1102	245-249	1.6011026106923332
1102	250-254	0.6440662785809046
1102	255-259	1.0076703216072112
1102	260-264	1.1191347461553676
1102	265-269	0.9119766680288386
1102	270-274	0.9343018357711692
1102	275-279	1.6914204902394694
1102	280-284	0.8128828398745789
1102	285-289	1.0802479469301716
1102	290-294	0.8724904419556303
1102	295-299	1.0563543712435628
1102	300-301	0.607674874137798
1103	1	0.04145650672358414
1103	2	0.05160640219833823
1103	3	0.034031972316967085
1103	4	0.05916588634782016
1103	5	0.045118484426765804
1103	6	0.14873369287668226
1103	7	0.12491012365361343
1103	8	0.14396789594336212
1103	9	0.13645431335902458
1103	10-14	0.0950432391190077
1103	15-19	0.058454787834243405
1103	20-24	0.04319158181836258
1103	25-29	0.00610224845047469
1103	30-34	0.17367344785107974
1103	35-39	-0.34263245586189583
1103	40-44	-0.21535164983753674
1103	45-49	-0.09476740479983192
1103	50-54	0.12320973101957833
1103	55-59	-0.22732460956683553
1103	60-64	0.22866403170858263
1103	65-69	0.3467570746717499
1103	70-74	0.2786209052211106
1103	75-79	0.3510313538551273
1103	80-84	0.37214032806956965
1103	85-89	0.24208082884257465
1103	90-94	0.4045220711549149
1103	95-99	0.35323774144895737
1103	100-104	0.3544841602187887
1103	105-109	0.1821020409416434
1103	110-114	0.33373901583617993
1103	115-119	0.21517394575000992
1103	120-124	0.5046760665487184
1103	125-129	0.3990353801782476
1103	130-134	0.43358556665744175
1103	135-139	0.4900812127155163
1103	140-144	0.5450156262138535
1103	145-149	0.9337325657545605
1103	150-154	0.9859200122673215
1103	155-159	0.17045419543695317
1103	160-164	-0.3024770965796648
1103	165-169	0.7475408600573488
1103	170-174	1.0015559488002594
1103	175-179	0.6237907228694368
1103	180-184	0.8699007556517628
1103	185-189	0.6298082764838568
1103	190-194	0.6472732145960975
1103	195-199	0.8271511810134768
1103	200-204	0.6747766958629597
1103	205-209	0.9666718005328789
1103	210-214	0.9407129892302137
1103	215-219	0.7218659136533496
1103	220-224	1.3575311336439349
1103	225-229	2.213372001246263
1103	230-234	1.0644431670835885
1103	235-239	1.3821871495411493
1103	240-244	1.2105625867710508
1103	245-249	1.4314501432472326
1103	250-254	1.636297350289773
1103	255-259	0.8143955310317175
1103	260-264	1.852601822861594
1103	265-269	0.8984818015319505
1103	270-274	1.25309738459951
1103	275-279	1.7018194379113787
1103	280-284	0.688524127334734
1103	285-289	0.9645803512676245
1103	290-294	1.4701585421590444
1103	295-299	1.5228164185582838
1103	300-301	-0.8419501238600553
2116	1	0.015289840056922799
2116	2	0.024058783150721297
2116	3	0.011452607237600887
2116	4	0.011800806982734002
2116	5	-0.02133389652561135
2116	6	-0.3658774182344331
2116	7	-0.28701844777495467
2116	8	0.013325038800509503
2116	9	0.005160662565373286
2116	10-14	0.09502260419836261
2116	15-19	0.09507066085011928
2116	20-24	-0.08495603722926148
2116	25-29	-0.20767552932730382
2116	30-34	0.03832741610505508
2116	35-39	-0.12229912252856678
2116	40-44	-0.16112149110737306
2116	45-49	0.03385005551763243
2116	50-54	0.18237957228941326
2116	55-59	0.14997380313156583
2116	60-64	0.3852703809149389
2116	65-69	0.2208253286399966
2116	70-74	-0.8167298884296841
2116	75-79	0.2754440522678223
2116	80-84	-0.1460390370097926
2116	85-89	0.13197447963622722
2116	90-94	0.2689157219485736
2116	95-99	0.18053932875054812
2116	100-104	0.22830320783783975
2116	105-109	0.29620997744958544
2116	110-114	0.38506599996316737
2116	115-119	0.287840612416673
2116	120-124	-0.09983821916556224
2116	125-129	0.058994110336975325
2116	130-134	0.15516651903838863
2116	135-139	0.44677803811234185
2116	140-144	0.4176632452614797
2116	145-149	0.6145865340085237
2116	150-154	0.774899377346685
2116	155-159	-0.37060612202336074
2116	160-164	-0.7769897949923603
2116	165-169	0.5484059394224232
2116	170-174	0.7358670599113708
2116	175-179	0.6132637387424538
2116	180-184	0.5975150413660515
2116	185-189	0.7437400225156026
2116	190-194	0.8850605161834011
2116	195-199	0.7818099111722105
2116	200-204	0.47046082284708746
2116	205-209	0.5404908481519293
2116	210-214	0.5410621955794177
2116	215-219	0.6944309930184325
2116	220-224	1.189151768564578
2116	225-229	0.6989212075954647
2116	230-234	0.9987336432740683
2116	235-239	1.0003823876363818
2116	240-244	1.3870117931202586
2116	245-249	0.6644739527710435
2116	250-254	1.0187910010834216
2116	255-259	0.5905415627777479
2116	260-264	0.009100235560001835
2116	265-269	0.5241516428017938
2116	270-274	0.81110849571062
2116	275-279	1.5002051521970934
2116	280-284	0.1922368257474325
2116	285-289	-1.1474894900022168
2116	290-294	0.1447283834288875
2116	295-299	0.974727529669396
2116	300-301	1.5718514634415328
1117	1	0.038123991185479156
1117	2	0.08374867520174689
1117	3	0.06032895659971871
1117	4	0.06750934476487913
1117	5	0.04376620160981304
1117	6	-0.3495830119537615
1117	7	-0.2640478884030202
1117	8	-0.05793698278928616
1117	9	0.0571155202690008
1117	10-14	0.10208776612182646
1117	15-19	0.11689754995708768
1117	20-24	0.09223768210342342
1117	25-29	0.1413274147354997
1117	30-34	0.10561730815607717
1117	35-39	0.014326981494981794
1117	40-44	-0.009357016131914975
1117	45-49	0.19652404962950243
1117	50-54	0.20391774697047538
1117	55-59	0.32602640372038394
1117	60-64	0.3624876527500618
1117	65-69	0.301203738846084
1117	70-74	-0.06170555084381846
1117	75-79	0.2939239345053153
1117	80-84	0.17360904934938048
1117	85-89	0.26984945510236713
1117	90-94	0.3596582145884071
1117	95-99	0.43465120313720007
1117	100-104	0.4386341204973121
1117	105-109	0.4650035005114148
1117	110-114	0.4079052541339152
1117	115-119	0.45887692252462386
1117	120-124	0.5786526542397326
1117	125-129	0.45569067559703313
1117	130-134	0.43770842286566847
1117	135-139	0.5331792157374693
1117	140-144	0.4794459734263441
1117	145-149	0.9498275546169666
1117	150-154	1.1344267571307896
1117	155-159	0.14563372095995675
1117	160-164	-0.2733996085350512
1117	165-169	0.4757651150848403
1117	170-174	0.7091341845433661
1117	175-179	0.9069110400182225
1117	180-184	0.6842337852325855
1117	185-189	1.150435213879689
1117	190-194	1.2315396133374747
1117	195-199	1.0625724234391356
1117	200-204	0.9945846697558238
1117	205-209	1.3221171484463348
1117	210-214	1.0745401151083698
1117	215-219	0.6057206887986055
1117	220-224	1.4060557921170762
1117	225-229	0.8276456433167603
1117	230-234	1.1880698552465887
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2108	190-194	-0.5609701517375747
2108	195-199	-1.110693381395997
2108	200-204	-0.5417598732959021
2108	205-209	-0.14434038420931827
2108	210-214	0.16954573273840268
2108	215-219	-0.8820474641781857
2108	220-224	-0.2838299812002383
2108	225-229	0.17002694607147717
2108	230-234	0.30383919360332357
2108	235-239	0.04647006402396414
2108	240-244	0.06913427665741523
2108	245-249	0.22327639867885196
2108	250-254	-0.10852282770302679
2108	255-259	-0.381257308341727
2108	260-264	0.3595147228600979
2108	265-269	-0.3356246507071461
2108	270-274	-0.24514004615203078
2108	275-279	0.0800162528556072
2108	280-284	-0.27664501809076114
2108	285-289	0.029354724485084915
2108	290-294	-0.09806126849961672
2108	295-299	0.20309498028087347
2108	300-301	-0.1073649053261061
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	28.0
14	145.0
15	308.0
16	419.0
17	581.0
18	1241.0
19	1211.0
20	1155.0
21	1473.0
22	2133.0
23	3238.0
24	4715.0
25	6546.0
26	9104.0
27	12190.0
28	16039.0
29	22388.0
30	31213.0
31	43675.0
32	59669.0
33	72370.0
34	63666.0
35	28093.0
36	2576.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.59211827789057	18.34426570878234	13.853662345775417	33.209953667551666
2	51.14009058253943	20.66297048258629	14.678796397521996	13.518142537352281
3	51.12473319798011	19.207142485293353	16.762975688479358	12.905148628247176
4	51.594825342287464	19.16887917122182	16.193450986516737	13.04284449997397
5	32.44703003800302	19.321411838200845	35.357124264667604	12.874433859128533
6	30.757978031131238	20.00208235722838	35.46462595658285	13.775313655057525
7	15.75797803113124	52.92206778072779	17.75547920245718	13.564474985683795
8	30.19886511531053	18.308084751939198	36.83872143266178	14.6543287000885
9	14.587953563433805	17.116195533343745	38.0860534124629	30.209797490759538
10-14	27.78093601957416	36.35644749856838	21.416575563537926	14.44604091831954
15-19	22.533395804050187	36.95866520901661	18.966838461138007	21.5411005257952
20-24	27.216304857098233	31.295174137123222	24.433390598157114	17.055130407621427
25-29	18.736842105263158	26.66151283252642	37.7409027018585	16.860742360351917
30-34	18.908636576604717	29.133010567962934	29.61148420011453	22.34686865531782
35-39	21.253735228278412	30.687542297881205	27.618564214691034	20.440158259149356
40-44	20.382112551408195	31.448175334478627	27.059034827424643	21.11067728668853
45-49	21.558436149721487	29.081732521214015	26.195689520537247	23.16414180852725
50-54	23.180175959185796	30.95944609297725	24.98323702431152	20.87714092352543
55-59	21.53636316310063	27.219948982247903	29.03815919621011	22.205528658441356
60-64	20.89426831172888	27.256234056952472	28.82612317143006	23.023374459888593
65-69	18.123119371128116	29.407257014940914	28.86069030142121	23.608933312509762
70-74	20.300692383778436	25.974699359675153	31.01650268103493	22.70810557551148
75-79	21.774949242542558	25.41189025977407	27.409495548961427	25.403664948721953
80-84	23.821646103389035	28.060284241761675	24.241449320630952	23.876620334218334
85-89	22.089697537612576	27.748086834296426	25.545057004529127	24.61715862356187
90-94	22.390702274975272	29.829663178718306	27.043573325003905	20.736061221302514
95-99	23.80936019574158	27.512832526419906	27.878182102139622	20.799625175698893
100-104	22.373991358217502	27.61387891092717	27.234941954292257	22.777187776563068
105-109	25.939195168931228	27.922015721797074	24.08610547139362	22.05268363787808
110-114	21.02644593680046	26.6760893331251	29.801395179343015	22.49606955073143
115-119	25.32765890988599	26.737206517778123	26.524545785829556	21.410588786506324
120-124	20.308501223384873	27.643864855015877	27.08782341610703	24.959810505492218
125-129	20.16455827997293	25.531729918267477	29.874277682336402	24.42943411942319
130-134	23.801186943620177	28.81529491384247	26.33015773856005	21.053360403977305
135-139	23.05805771151945	26.7864682109783	24.80952928810937	25.34594478939288
140-144	22.465458899474207	27.459315945650474	26.17044093914311	23.904784215732207
145-149	22.567129991149983	25.249049924514548	29.447186214795146	22.73663386954032
150-154	21.748399187880683	28.43016294445312	25.497839554375552	24.323598313290645
155-159	21.511583112082878	27.468009787078973	30.06793690457598	20.95247019626217
160-164	23.598169398807535	30.372133048119654	24.19762829800005	21.832069255072756
165-169	21.732428409822692	28.54038655858533	26.90641204977461	22.820772981817367
170-174	21.66255401114061	31.177885366234577	26.135405278775576	21.024155343849237
175-179	20.231818418449684	28.182674787859856	27.435056483939817	24.15045030975064
180-184	21.176271539382583	28.54963819043157	27.99630381591962	22.27778645426623
185-189	20.648612967157565	27.407791872451387	28.26056966035174	23.683025500039303
190-194	20.08787547503774	26.180436253839346	28.726898849497633	25.00478942162528
195-199	18.818418449685044	31.99187880680931	26.788588682388465	22.401114061117184
200-204	21.508251340517464	27.139934405747308	25.586964443750325	25.764849809984902
205-209	21.816752563902337	25.964704044978916	29.827580821489924	22.39096256962882
210-214	23.169255645403535	25.79526565425876	29.317418562870824	21.718060137466885
215-219	21.985059086886356	27.25165287105003	29.369045759800095	21.394242282263523
220-224	20.048570982352025	26.869436201780417	28.483263053776874	24.598729762090688
225-229	23.408818782862202	26.401947004008537	27.991097922848663	22.198136290280598
230-234	21.762049956817094	26.357554457574288	28.86841005175181	23.011985533856805
235-239	22.35025248581394	26.701962621687752	26.332812744026235	24.61497214847207
240-244	23.015825914935707	29.859701181737723	26.356968087875476	20.76750481545109
245-249	24.11773035319939	26.650920559030205	25.798038209212564	23.43331087855784
250-254	21.335311572700295	26.043781560726742	25.138893227133114	27.482013639439845
255-259	22.972200531001093	28.178197719818836	25.278046748919774	23.571555000260293
260-264	20.18190441038285	27.40664657537955	27.467919968722974	24.943529045514627
265-269	20.515331355093966	27.36566192930397	26.749544484356292	25.36946223124577
270-274	20.092456660940183	30.172106824925816	25.723827372585767	24.01160914154823
275-279	21.068353376021655	27.332864802956948	25.790306627101877	25.808475193919516
280-284	17.63990837628195	29.280597636524547	29.306158571502944	23.77333541569056
285-289	20.84991410276433	26.740798583997083	26.757353323962725	25.65193398927586
290-294	21.58618355978968	28.513196938934875	26.228070175438596	23.672549325836847
295-299	20.393721692956426	28.02509240460201	25.95804050184809	25.623145400593472
300-301	17.187776563069395	30.33044406267895	27.55427143526472	24.927507938986935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.25
27	0.5
28	0.75
29	1.25
30	1.75
31	2.5
32	11.0
33	59.75
34	191.0
35	325.75
36	494.5
37	974.0
38	2350.5
39	5659.25
40	7455.5
41	7227.0
42	9957.25
43	12224.5
44	14372.25
45	12836.25
46	13741.75
47	12727.75
48	8422.75
49	6476.0
50	4942.5
51	1604.75
52	346.75
53	168.25
54	167.75
55	256.25
56	365.25
57	385.0
58	696.0
59	1087.25
60	1065.75
61	911.25
62	488.5
63	113.5
64	20.25
65	6.5
66	3.0
67	2.5
68	1.0
69	0.25
70	1.5
71	0.5
72	0.0
73	0.5
74	0.25
75	0.25
76	0.0
77	0.0
78	0.0
79	0.0
80	0.25
81	0.0
82	0.0
83	0.25
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.25
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	5.2058930709563226E-5
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	5.2058930709563226E-5
165-169	5.2058930709563226E-5
170-174	0.0
175-179	0.0
180-184	0.0
185-189	5.2058930709563226E-5
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	5.2058930709563226E-5
215-219	0.0
220-224	0.0
225-229	0.0
230-234	5.2058930709563226E-5
235-239	0.0
240-244	0.0
245-249	1.0411786141912645E-4
250-254	0.0
255-259	0.0
260-264	5.2058930709563226E-5
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	384180.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.177001210622146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	40.03243865935616	4.074101767004245
2	9.159602991041456	1.8643458145729426
3	6.544666122554453	1.9981522515706323
4	5.433462505771509	2.2118541799642673
5	5.311304321918556	2.702657525707389
6	4.3302370275658415	2.6441296483091046
7	4.111559310495723	2.929034085632181
8	3.13824796015999	2.555036263182456
9	2.6283530793459633	2.4073877223401685
>10	17.972255195517654	34.77801509741487
>50	1.0977146590778113	7.396612548269791
>100	0.15602193369115122	2.5815928193954742
>500	0.02294624550105404	1.6878593482478652
>1k	0.04079332533512622	11.498460725209318
>5k	0.01529749700067233	11.453053286939847
>10k+	0.005099165666890777	7.217706916239368
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	13957	3.632932479566869	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	13860	3.607683898172732	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	9328	2.428028528294029	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	8645	2.2502472799208704	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	8456	2.201051590400333	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	7074	1.8413243791972511	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	5509	1.4339632463949192	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	5128	1.3347909833932012	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACTAAAGCAACAAAAATGAAGGCAATA	4747	1.2356187203914832	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	4413	1.1486803061065125	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA	3988	1.0380550783486908	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	3816	0.9932843979384663	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	3239	0.8430943828413764	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	3006	0.7824457285647353	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	2889	0.7519912540996407	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	2574	0.6699984382320787	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	2550	0.6637513665469311	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	2410	0.6273101150502369	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	2343	0.6098703732625331	No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	2297	0.5978968191993337	No Hit
GGGGGGAGCAAAAGCAGGGGAAAACTAAAGCAACAAAAATGAAGGCAATA	1959	0.5099172263001718	No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	1477	0.3844552032901244	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT	1397	0.36363163100629914	TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	1210	0.3149565307928575	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	902	0.23478577750013016	No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	849	0.22099016086209589	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT	848	0.2207298662085481	No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA	795	0.2069342495705138	No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	766	0.19938570461762714	No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTACCGAAAGGAAAACTCATCTTG	632	0.1645062210422198	No Hit
GGGGGGAGCGAAAGCAGGAGTGGACAGATTCTACAGGACATGCAAGTTAG	573	0.14914883648289864	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	571	0.148628247175803	No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	569	0.1481076578687074	No Hit
GGGGGGAGCGAAAGCAGGCTTGACTGCGAGGCCTACAAGCCGAGCAGATG	497	0.12936644281326462	No Hit
GGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGT	452	0.1176531834036129	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	7.808839606434484E-4	0.0	0.0
9	7.808839606434484E-4	0.0	0.0
10-14	0.00161382685199646	0.0	0.0
15-19	0.0026029465354781615	0.0	0.0
20-24	0.003383830496121609	0.0	0.0
25-29	0.003592066218959863	0.0	0.0
30-34	0.00499765734811807	0.0	0.0
35-39	0.005518246655213702	0.0	0.0
40-44	0.00676766099224322	0.0	0.0
45-49	0.00838148784423968	0.0	0.0
50-54	0.009943255765526576	0.0	0.0
55-59	0.013431204123067314	0.0	0.0
60-64	0.01780415430267062	0.0	0.0
65-69	0.028632411890259772	0.0	0.0
70-74	0.04107449632984538	0.0	0.0
75-79	0.06267895257431413	0.0	0.0
80-84	0.09391431100005206	0.0	0.0
85-89	0.133374980477901	0.0	0.0
90-94	0.18111301993857046	0.0	0.0
95-99	0.23650372221354576	0.0	0.0
100-104	0.29569472643031913	0.0	0.0
105-109	0.3632151595606226	0.0	0.0
110-114	0.43849237336665103	0.0	0.0
115-119	0.5231922536311104	0.0	0.0
120-124	0.6282471758030089	0.0	0.0
125-129	0.7411629965120516	0.0	0.0
130-134	0.85340205112187	0.0	0.0
135-139	0.9897443906502161	0.0	0.0
140-144	1.1301993857046175	0.0	0.0
145-149	1.2926232495184549	0.0	0.0
150-154	1.4631162475922745	0.0	0.0
155-159	1.6562548805247541	0.0	0.0
160-164	1.880108282575876	0.0	0.0
165-169	2.1448800041647145	0.0	0.0
170-174	2.4322453016815033	0.0	0.0
175-179	2.739861523244312	0.0	0.0
180-184	3.0630954240199904	0.0	0.0
185-189	3.4404185538029046	0.0	0.0
190-194	3.8579832370243117	0.0	0.0
195-199	4.308501223384871	0.0	0.0
200-204	4.791035452131813	0.0	0.0
205-209	5.349471601853298	0.0	0.0
210-214	6.0325888906241865	0.0	0.0
215-219	6.614347441303555	0.0	0.0
220-224	7.255713467645374	0.0	0.0
225-229	7.9672549325836854	0.0	0.0
230-234	8.724035608308606	0.0	0.0
235-239	9.499297204435422	0.0	0.0
240-244	10.270029673590503	0.0	0.0
245-249	11.028476235098129	0.0	0.0
250-254	11.837263782601905	0.0	0.0
255-259	12.647352803373419	0.0	0.0
260-264	13.463636836899369	0.0	0.0
265-269	14.24066843667031	0.0	0.0
270-274	14.996772346296007	0.0	0.0
275-279	15.674163152688843	0.0	0.0
280-284	16.338903638919255	0.0	0.0
285-289	16.98688114946119	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	7155	0.0	291.49548	1
GGGGGGA	6040	0.0	288.89487	1
GGGTTAT	7215	0.0	288.8669	2
ATTAGTA	7355	0.0	283.36847	7
GGTTATT	7430	0.0	280.5081	3
TTATTAG	7520	0.0	277.34708	5
TATTAGT	7550	0.0	276.24503	6
GTTATTA	7585	0.0	275.1648	4
TAGTAGA	7610	0.0	274.06702	9
TTAGTAG	7650	0.0	272.8268	8
GGAGCGA	4790	0.0	266.0543	5
GGGGGAG	6660	0.0	262.44366	2
GAGCGAA	4880	0.0	260.84528	6
CGAAAGC	4960	0.0	256.34073	9
GCGAAAG	5000	0.0	254.29	8
GGGAGCG	5385	0.0	236.65738	4
GGGGAGC	7380	0.0	236.03998	3
AGCGAAA	5725	0.0	222.60262	7
TCGAACC	1185	0.0	180.48523	295
GGCCTGC	1235	0.0	174.37247	295
>>END_MODULE