FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_30304_flu.3510000003baa0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_30304_flu.3510000003baa0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384180
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA139573.632932479566869No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC138603.607683898172732No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA93282.428028528294029No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC86452.2502472799208704No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG84562.201051590400333No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG70741.8413243791972511No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG55091.4339632463949192No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG51281.3347909833932012No Hit
GGGGGGAGCGAAAGCAGGGGAAAACTAAAGCAACAAAAATGAAGGCAATA47471.2356187203914832No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT44131.1486803061065125No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA39881.0380550783486908No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC38160.9932843979384663No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA32390.8430943828413764No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA30060.7824457285647353No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG28890.7519912540996407No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT25740.6699984382320787No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG25500.6637513665469311No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT24100.6273101150502369No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG23430.6098703732625331No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA22970.5978968191993337No Hit
GGGGGGAGCAAAAGCAGGGGAAAACTAAAGCAACAAAAATGAAGGCAATA19590.5099172263001718No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC14770.3844552032901244No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT13970.36363163100629914TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA12100.3149565307928575No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC9020.23478577750013016No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA8490.22099016086209589No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT8480.2207298662085481No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA7950.2069342495705138No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA7660.19938570461762714No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTACCGAAAGGAAAACTCATCTTG6320.1645062210422198No Hit
GGGGGGAGCGAAAGCAGGAGTGGACAGATTCTACAGGACATGCAAGTTAG5730.14914883648289864No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC5710.148628247175803No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA5690.1481076578687074No Hit
GGGGGGAGCGAAAGCAGGCTTGACTGCGAGGCCTACAAGCCGAGCAGATG4970.12936644281326462No Hit
GGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGT4520.1176531834036129No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA71550.0291.495481
GGGGGGA60400.0288.894871
GGGTTAT72150.0288.86692
ATTAGTA73550.0283.368477
GGTTATT74300.0280.50813
TTATTAG75200.0277.347085
TATTAGT75500.0276.245036
GTTATTA75850.0275.16484
TAGTAGA76100.0274.067029
TTAGTAG76500.0272.82688
GGAGCGA47900.0266.05435
GGGGGAG66600.0262.443662
GAGCGAA48800.0260.845286
CGAAAGC49600.0256.340739
GCGAAAG50000.0254.298
GGGAGCG53850.0236.657384
GGGGAGC73800.0236.039983
AGCGAAA57250.0222.602627
TCGAACC11850.0180.48523295
GGCCTGC12350.0174.37247295
Produced by FastQC (version 0.11.2)