##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_30301_flu.3510000003b9e4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	345555
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.81699874115553	34.0	34.0	34.0	34.0	34.0
2	33.881428426733805	34.0	34.0	34.0	34.0	34.0
3	33.88966734673207	34.0	34.0	34.0	34.0	34.0
4	33.90389084226823	34.0	34.0	34.0	34.0	34.0
5	33.893600150482555	34.0	34.0	34.0	34.0	34.0
6	37.676219415143755	38.0	38.0	38.0	38.0	38.0
7	37.68484901101127	38.0	38.0	38.0	38.0	38.0
8	37.684941615661764	38.0	38.0	38.0	38.0	38.0
9	37.600445659880485	38.0	38.0	38.0	38.0	38.0
10-14	37.67837594594203	38.0	38.0	38.0	38.0	38.0
15-19	37.64538496042598	38.0	38.0	38.0	37.8	38.0
20-24	37.64850805226375	38.0	38.0	38.0	37.8	38.0
25-29	37.558465656697194	38.0	38.0	38.0	37.0	38.0
30-34	37.50948879339034	38.0	38.0	38.0	37.0	38.0
35-39	37.42994429251494	38.0	38.0	38.0	37.0	38.0
40-44	37.436692856419384	38.0	38.0	38.0	37.0	38.0
45-49	37.40958168743036	38.0	38.0	38.0	37.0	38.0
50-54	37.37730723039748	38.0	38.0	38.0	37.0	38.0
55-59	37.33381487751588	38.0	38.0	38.0	37.0	38.0
60-64	37.16412727351651	38.0	38.0	38.0	36.6	38.0
65-69	37.09637365976472	38.0	38.0	38.0	36.6	38.0
70-74	37.18024974316678	38.0	38.0	38.0	37.0	38.0
75-79	37.11073374716037	38.0	38.0	38.0	37.0	38.0
80-84	37.01652935133336	38.0	38.0	38.0	36.4	38.0
85-89	37.05606227662746	38.0	38.0	38.0	36.8	38.0
90-94	36.910211688443226	38.0	38.0	38.0	35.8	38.0
95-99	36.92178263952193	38.0	38.0	38.0	36.0	38.0
100-104	36.90584248527731	38.0	38.0	38.0	36.0	38.0
105-109	36.76109968022457	38.0	38.0	38.0	35.8	38.0
110-114	36.66578808004515	38.0	38.0	38.0	35.0	38.0
115-119	36.768415447613265	38.0	38.0	38.0	35.6	38.0
120-124	36.683304249685285	38.0	37.8	38.0	34.8	38.0
125-129	36.59607992938895	38.0	37.4	38.0	34.6	38.0
130-134	36.48501106914963	38.0	37.0	38.0	34.0	38.0
135-139	36.24684464122933	38.0	37.0	38.0	34.0	38.0
140-144	36.21198477811057	38.0	37.0	38.0	34.0	38.0
145-149	35.80900753859733	38.0	37.0	38.0	30.8	38.0
150-154	35.43867748983519	38.0	36.4	38.0	28.8	38.0
155-159	35.554539219516435	38.0	36.4	38.0	29.6	38.0
160-164	35.51331915324623	38.0	36.0	38.0	29.6	38.0
165-169	35.3063466018434	38.0	36.0	38.0	28.4	38.0
170-174	35.16593537931733	38.0	35.6	38.0	28.0	38.0
175-179	34.826093096612695	38.0	35.2	38.0	26.0	38.0
180-184	34.55120603087786	37.6	35.0	38.0	24.4	38.0
185-189	33.789297796298705	37.0	33.8	37.8	22.8	38.0
190-194	32.796015684912675	36.8	31.8	37.0	21.2	38.0
195-199	32.57410484582772	36.8	30.8	37.2	20.6	37.8
200-204	32.380365498979906	37.0	30.6	37.0	20.0	37.4
205-209	31.300293730375774	35.4	28.6	37.0	18.2	37.2
210-214	30.585395667838696	34.6	27.0	37.0	16.6	37.0
215-219	29.706234897483757	33.4	24.8	37.0	14.4	37.0
220-224	28.224555280635503	31.6	22.4	36.2	9.6	37.0
225-229	27.188471299793086	30.4	20.2	36.2	9.2	37.0
230-234	26.784072000115753	29.6	20.0	36.0	9.0	37.0
235-239	25.709626542807946	28.2	18.4	34.6	8.8	37.0
240-244	24.613152754264878	26.6	16.6	33.8	8.0	37.0
245-249	23.711244809075254	25.0	15.2	33.0	8.0	36.4
250-254	22.796316071247706	23.8	14.0	32.0	8.0	36.0
255-259	22.160084501743572	22.4	13.2	31.2	8.0	36.0
260-264	20.853271982752382	20.6	11.6	29.4	7.8	35.2
265-269	20.027995543401197	19.6	10.2	28.4	7.0	34.0
270-274	18.963576854625167	18.2	8.6	26.6	7.0	33.2
275-279	17.667407503870585	16.2	8.0	25.0	7.0	31.2
280-284	17.380305305957084	16.2	8.0	24.4	7.0	30.8
285-289	16.247028692972172	14.0	8.0	22.2	7.0	29.4
290-294	15.536915396970093	13.0	8.0	20.8	7.0	28.4
295-299	14.769765160394149	13.0	8.0	19.8	7.0	27.0
300-301	13.02907207246314	10.0	8.0	16.5	7.0	22.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.018953243623613503
1101	2	0.0741103252727271
1101	3	0.054321738483409376
1101	4	-0.0027224722885392794
1101	5	0.03824440986743127
1101	6	0.17590170952455253
1101	7	0.12468434767227166
1101	8	0.0462414042777155
1101	9	0.020384037689204604
1101	10-14	0.08782319939382432
1101	15-19	0.08683952611080059
1101	20-24	0.07136333699552466
1101	25-29	0.10820828747303324
1101	30-34	0.12556823954420793
1101	35-39	0.18025027561526485
1101	40-44	0.20029707619961812
1101	45-49	0.18554301975209597
1101	50-54	0.2106215362418169
1101	55-59	0.16202304915475452
1101	60-64	0.29229879279843374
1101	65-69	0.29387795905141445
1101	70-74	0.2822362354110126
1101	75-79	0.16197841653860223
1101	80-84	0.29973710920819485
1101	85-89	0.13780444800535463
1101	90-94	0.29672868429346266
1101	95-99	0.23165217015075257
1101	100-104	0.1382589116041828
1101	105-109	0.06378372419491996
1101	110-114	0.11118129313012304
1101	115-119	0.2130397990262196
1101	120-124	0.20920402657662152
1101	125-129	0.33108451004981987
1101	130-134	0.2603312200917358
1101	135-139	0.3061721327455942
1101	140-144	0.27703392977662844
1101	145-149	0.4557856311789621
1101	150-154	0.7178062282088362
1101	155-159	0.3982066679813556
1101	160-164	0.3964502957060816
1101	165-169	0.25761443986699817
1101	170-174	0.32736564064003915
1101	175-179	0.5812477571829291
1101	180-184	0.25336052800732034
1101	185-189	0.4696334357120975
1101	190-194	0.4914922248099032
1101	195-199	0.3576107214928541
1101	200-204	-0.09578856328547403
1101	205-209	-0.041270708768536224
1101	210-214	-0.46453531663547665
1101	215-219	-0.33755346085557036
1101	220-224	0.5554360841457964
1101	225-229	0.6475395851793486
1101	230-234	0.33660029563024096
1101	235-239	0.4186559358229367
1101	240-244	0.4607253773896751
1101	245-249	0.4839744174123304
1101	250-254	0.020325947111036413
1101	255-259	-0.3094547507739698
1101	260-264	0.33572430502547235
1101	265-269	-0.09189057476699247
1101	270-274	0.20848425153063488
1101	275-279	0.4717918914711774
1101	280-284	0.0973063945951047
1101	285-289	0.09725652759064118
1101	290-294	0.07113670703521002
1101	295-299	0.03894970947305154
1101	300-301	0.06409123187615151
1102	1	-0.02600974570368919
1102	2	-0.007075469899454845
1102	3	-0.04700909412034093
1102	4	0.005591720939222
1102	5	0.010394487832073196
1102	6	0.07173679813072908
1102	7	0.17023963052456992
1102	8	0.04151346240990961
1102	9	0.10173467587588192
1102	10-14	0.10841880773170942
1102	15-19	0.12002352388359583
1102	20-24	0.09395098158421433
1102	25-29	0.10756918500050716
1102	30-34	0.11406708136939869
1102	35-39	0.15129578327680093
1102	40-44	0.09157517124931758
1102	45-49	0.12936769516398527
1102	50-54	0.12786643557158328
1102	55-59	0.09461104785066965
1102	60-64	0.28018319755031484
1102	65-69	0.33883492281402994
1102	70-74	0.18722829241983163
1102	75-79	0.10575424957583124
1102	80-84	0.1606318282485404
1102	85-89	0.19671812032918723
1102	90-94	0.33433926648235257
1102	95-99	0.21698822400436057
1102	100-104	0.2031367262986521
1102	105-109	0.21217247530761085
1102	110-114	0.3293237932003379
1102	115-119	0.19219875775563366
1102	120-124	0.3368557706767703
1102	125-129	0.23046995087056388
1102	130-134	0.2899165116990261
1102	135-139	0.3544318178832384
1102	140-144	0.29567511049219775
1102	145-149	0.46351371591070034
1102	150-154	0.9389618143722771
1102	155-159	0.5722383984300592
1102	160-164	0.537957400440007
1102	165-169	0.7584820025470194
1102	170-174	0.5292299540810887
1102	175-179	0.7621862878333587
1102	180-184	0.7281711722260269
1102	185-189	0.7304553796141917
1102	190-194	0.7817453626272126
1102	195-199	0.6017158742412789
1102	200-204	0.4123044960909823
1102	205-209	0.21001744781034404
1102	210-214	-0.09400778235728069
1102	215-219	0.14111450990249708
1102	220-224	0.7651212828304672
1102	225-229	1.2905279570309993
1102	230-234	1.0029519289802984
1102	235-239	1.1217179863967743
1102	240-244	1.0739963390105558
1102	245-249	1.2186142842680212
1102	250-254	0.738365267664765
1102	255-259	0.5547075231316221
1102	260-264	1.3232892806470211
1102	265-269	1.2935751555913484
1102	270-274	0.9056205009979479
1102	275-279	1.0552357917301585
1102	280-284	0.6009406335881664
1102	285-289	1.1541532540525843
1102	290-294	0.9259866937867169
1102	295-299	0.9936496280283915
1102	300-301	0.5606283075122303
1103	1	-0.06845679141160588
1103	2	0.0016759236345507134
1103	3	-0.08003584997318569
1103	4	0.01540220031491657
1103	5	0.07784153353998846
1103	6	0.045538358888812525
1103	7	0.08161086798276074
1103	8	-0.007176615627997762
1103	9	0.15585953652248463
1103	10-14	0.049594950429586504
1103	15-19	0.027983390104331818
1103	20-24	0.04786959919848499
1103	25-29	0.09023362201276797
1103	30-34	0.09567800667597481
1103	35-39	-0.4776841498335713
1103	40-44	-0.36024199708958804
1103	45-49	-0.25980175857068843
1103	50-54	-0.0216597628676638
1103	55-59	-0.2561916277346157
1103	60-64	0.1059858731356016
1103	65-69	0.26239122158771977
1103	70-74	0.042423387180150485
1103	75-79	0.16415447254126292
1103	80-84	0.1836530099653757
1103	85-89	0.14560886726341238
1103	90-94	0.24927237685025005
1103	95-99	0.16570617272230947
1103	100-104	0.14474207746921763
1103	105-109	0.008543712765806788
1103	110-114	0.15906738294392397
1103	115-119	-0.047472368219835914
1103	120-124	0.19109768817956052
1103	125-129	0.08167953838449904
1103	130-134	0.23127102674919797
1103	135-139	0.24517875210842277
1103	140-144	0.30544099677981507
1103	145-149	0.6474211852529663
1103	150-154	0.7876407441381659
1103	155-159	-0.07801243769034727
1103	160-164	-0.6330961112656723
1103	165-169	0.25283540276998906
1103	170-174	0.5550214813943555
1103	175-179	0.44803355650671506
1103	180-184	0.4909192212784248
1103	185-189	0.4261075535272312
1103	190-194	0.382871338101026
1103	195-199	0.4793134216214341
1103	200-204	0.4401138606394852
1103	205-209	0.6400174478103438
1103	210-214	0.87080827115442
1103	215-219	0.5779929937374995
1103	220-224	1.2723509372340374
1103	225-229	2.0341344230287675
1103	230-234	1.0127401118119614
1103	235-239	1.108891899440252
1103	240-244	0.7978090480406586
1103	245-249	1.2931181861632268
1103	250-254	1.5362247994373384
1103	255-259	0.5406216814370843
1103	260-264	1.5034063375032112
1103	265-269	0.6693499604965893
1103	270-274	1.1619137005520166
1103	275-279	1.7308377984191274
1103	280-284	0.4186162188724474
1103	285-289	0.5456359742309544
1103	290-294	1.0228484552136958
1103	295-299	1.2082594385077687
1103	300-301	-0.9341598753194305
2116	1	-0.0012200870067005098
2116	2	-0.035242542734017945
2116	3	0.01718372951497571
2116	4	0.05099348563506112
2116	5	0.012053311677178158
2116	6	-0.2156166216078148
2116	7	-0.13658911298955445
2116	8	0.015896831237192544
2116	9	-0.01859103906499371
2116	10-14	0.06729022204979174
2116	15-19	0.08625377351218333
2116	20-24	-0.1513022207996073
2116	25-29	-0.23345108924209512
2116	30-34	-0.06331875861753389
2116	35-39	-0.1937259156027622
2116	40-44	-0.1691146067366276
2116	45-49	0.09365224200014666
2116	50-54	0.14652356888003482
2116	55-59	0.09297686241855274
2116	60-64	0.3392809925343272
2116	65-69	0.20132281705912902
2116	70-74	-0.8375242866853299
2116	75-79	0.16713658651710261
2116	80-84	-0.15159102009837255
2116	85-89	0.04983634324096187
2116	90-94	0.23960773980334693
2116	95-99	0.18225074507508054
2116	100-104	0.20055408393863416
2116	105-109	0.19722842402067897
2116	110-114	0.37486607003363304
2116	115-119	0.17958071902209127
2116	120-124	-0.2698922271472455
2116	125-129	-0.08589776158695628
2116	130-134	0.09825941986117215
2116	135-139	0.4395085018943732
2116	140-144	0.3817439175295121
2116	145-149	0.4981589837497751
2116	150-154	0.84063332334091
2116	155-159	-0.47086516340625906
2116	160-164	-0.8509055489975808
2116	165-169	0.34301664147229616
2116	170-174	0.6329679184602881
2116	175-179	0.3346461528343667
2116	180-184	0.46495071209756134
2116	185-189	0.7100767286770733
2116	190-194	0.5006308281590535
2116	195-199	0.5662019193856835
2116	200-204	0.6679860897328069
2116	205-209	0.5446288073384657
2116	210-214	0.3530820795456293
2116	215-219	0.605132181255339
2116	220-224	0.9584935497352234
2116	225-229	0.8805291523599443
2116	230-234	0.9150386561940422
2116	235-239	0.7300996817635337
2116	240-244	1.5621816101184827
2116	245-249	0.6154861815965802
2116	250-254	0.7639062759857183
2116	255-259	0.7060298252863859
2116	260-264	0.08562544129923211
2116	265-269	0.43642159973167693
2116	270-274	0.3098839222245502
2116	275-279	0.9950995242301843
2116	280-284	-0.35289600641534236
2116	285-289	-1.4532109480409474
2116	290-294	0.2136147952384313
2116	295-299	0.6118732716349697
2116	300-301	1.3955399629452447
1117	1	-0.04440072733837752
1117	2	0.0751503706215857
1117	3	0.08276926062941214
1117	4	0.06625078917838323
1117	5	0.039273646430906695
1117	6	-0.3450985594941045
1117	7	-0.1814516796907668
1117	8	0.11831918910122141
1117	9	0.15045335803278448
1117	10-14	0.12155909993377634
1117	15-19	0.09444410711424922
1117	20-24	0.058771582417406876
1117	25-29	0.1718888470318518
1117	30-34	0.12057121796506465
1117	35-39	-0.16688323450176057
1117	40-44	-0.033209197699811455
1117	45-49	0.13838092640261834
1117	50-54	0.08670217458466567
1117	55-59	0.23955398630047142
1117	60-64	0.16495040402804761
1117	65-69	0.33268870442525156
1117	70-74	-0.12734587292663946
1117	75-79	0.07698437338031994
1117	80-84	-0.004743252429747713
1117	85-89	0.027061956508262597
1117	90-94	0.15222050804780451
1117	95-99	0.17940754572001794
1117	100-104	0.13788853055847028
1117	105-109	0.2324586631853336
1117	110-114	0.320163874171989
1117	115-119	0.23670383315315036
1117	120-124	0.37641233348848857
1117	125-129	0.255779462106851
1117	130-134	0.11486370409473778
1117	135-139	0.3736398505065992
1117	140-144	0.4209978236295555
1117	145-149	0.7677053195016299
1117	150-154	0.9602799508505981
1117	155-159	-0.03147353608850523
1117	160-164	-0.5089408862466129
1117	165-169	0.30470039132833904
1117	170-174	0.567584410456142
1117	175-179	0.940670856277876
1117	180-184	0.8143326461449405
1117	185-189	0.8994961881572863
1117	190-194	1.1009739315411409
1117	195-199	0.9371585779906226
1117	200-204	0.7690249971764729
1117	205-209	1.2040010481154582
1117	210-214	0.7958788279812694
1117	215-219	0.6054129022043178
1117	220-224	1.163556124114276
1117	225-229	0.6337759180707252
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2108	170-174	-0.5547442243057148
2108	175-179	-0.5305451832810562
2108	180-184	-0.2067749284069933
2108	185-189	-0.8099052176250012
2108	190-194	-0.40981999441846995
2108	195-199	-1.3751315966901423
2108	200-204	-0.7947405857874159
2108	205-209	0.060303859680004734
2108	210-214	0.23582094840111267
2108	215-219	-0.6628333749356123
2108	220-224	0.0033073650676342936
2108	225-229	0.2976281783109549
2108	230-234	-0.003567522707708548
2108	235-239	-0.2062095185332673
2108	240-244	0.07725124918630044
2108	245-249	0.15743314531431807
2108	250-254	-0.024143310244323146
2108	255-259	-0.42415482339905
2108	260-264	-0.03455074766910471
2108	265-269	-0.6957725862214126
2108	270-274	-0.8167523713796179
2108	275-279	-0.18485898062116135
2108	280-284	-0.4749451483920808
2108	285-289	-0.0032343422306801983
2108	290-294	-0.5358561179856665
2108	295-299	-0.05556674235428538
2108	300-301	-0.3335612275590769
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	3.0
13	18.0
14	78.0
15	221.0
16	302.0
17	494.0
18	907.0
19	833.0
20	839.0
21	1109.0
22	1553.0
23	2513.0
24	3728.0
25	5480.0
26	7656.0
27	10537.0
28	14468.0
29	20223.0
30	28508.0
31	40094.0
32	55097.0
33	66436.0
34	58261.0
35	24161.0
36	2032.0
37	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.150366801232803	24.439235432854396	15.797485204960138	31.612912560952672
2	40.62566016987166	25.097017840864694	18.849676607197118	15.42764538206653
3	39.896109157731765	23.89228921589906	20.52176932760342	15.689832298765754
4	40.53074040311962	23.36994110923008	20.31427703259973	15.785041455050571
5	27.07875736134624	23.58785142741387	33.94944364862323	15.38394756261666
6	25.690845162130486	23.319587330526254	34.47671137734948	16.51285612999378
7	18.11694231019664	44.56859255400732	21.368523100519454	15.945942035276584
8	25.274413624459203	22.155952019215466	35.52632721274471	17.043307143580616
9	16.611248571139182	20.59845755379028	37.11189246284962	25.678401412220918
10-14	25.489314291502076	33.311686996281345	24.245460201704503	16.953538510512075
15-19	21.211905485378594	34.65352838187843	22.713084747724675	21.421481385018303
20-24	24.740200546946216	30.555830475611696	26.528859371156543	18.17510960628554
25-29	19.846044768560724	26.01281995630218	35.95473947707312	18.186395798063984
30-34	20.08965287725543	28.311672526804703	30.559129516285395	21.039545079654467
35-39	20.93333912112399	29.80208649853135	27.83238558261348	21.432188797731186
40-44	20.73834845393642	28.970438859226462	28.6894416228965	21.601771063940618
45-49	21.296812374296422	30.003327979626977	26.693579893215265	22.00627975286134
50-54	21.957025654382083	29.563629523520135	27.716571891594683	20.762772930503104
55-59	21.53231757607327	27.390198376524722	29.624574959123727	21.452909088278275
60-64	22.404479749967443	26.204222193283265	28.397447584320872	22.993850472428413
65-69	20.648464065054768	27.920186366859113	28.431364037562762	22.99998553052336
70-74	21.154722113701148	26.955361664568596	29.137648131267092	22.75226809046317
75-79	20.549319211124136	26.747001200966565	29.940125305667692	22.76355428224161
80-84	22.91455774044653	26.47248629017088	27.853221628973678	22.759734340408908
85-89	21.393410600338587	28.063955086744514	26.630782364601874	23.91185194831503
90-94	20.528598920577043	29.881321352606676	28.715891826192646	20.874187900623635
95-99	22.28513550664872	29.97629899726527	26.961901868009434	20.776663628076573
100-104	22.679689195641796	28.305711102429427	26.99315593754974	22.021443764379043
105-109	23.32861628394901	27.730115321728814	24.705589558825658	24.235678835496522
110-114	21.778125045217113	28.159858777908003	29.182503508848086	20.8795126680268
115-119	23.295104976053015	27.806224768850115	27.28295061567623	21.615719639420643
120-124	18.974924396984562	29.088857056040286	27.86433418703245	24.0718843599427
125-129	21.100606271071175	25.789295481182446	29.731591208345993	23.378507039400382
130-134	24.35936392180695	28.958747522102122	26.314711116898902	20.367177439192023
135-139	23.9295625877212	27.68005093255777	25.420613216419962	22.969773263301065
140-144	21.187712520438133	29.186728596026683	27.009419629292008	22.616139254243173
145-149	21.112066096569286	27.14334910506287	28.846117115943915	22.898467682423927
150-154	22.845220008392296	26.178929548118248	26.0385177468131	24.93733269667636
155-159	21.424722547785446	27.254069540304727	29.872060887557694	21.44914702435213
160-164	24.681743861324538	28.924252289794676	26.049225159525978	20.344778689354808
165-169	20.463081130355516	26.345675796906427	28.17467552198637	25.016567550751688
170-174	22.196061408458856	29.18371894488576	26.308286669271176	22.31193297738421
175-179	20.459376944335926	29.264632258251215	28.48611653716485	21.789874260248006
180-184	20.03356918580255	29.786285830041525	26.596287132294428	23.5838578518615
185-189	21.858806846956348	28.286322003733126	27.467523259683695	22.38734788962683
190-194	20.608528309531042	28.46423868848664	26.480126173836293	24.447106828146026
195-199	21.082085340973215	30.08713518832024	27.290069598182633	21.540709872523912
200-204	21.47849112297608	28.602393251436098	26.99182474569895	22.927290879888872
205-209	21.294497258034177	28.291531015323173	27.474873753816325	22.93909797282632
210-214	22.5790395161407	28.155344301196628	27.16974143045246	22.09587475221021
215-219	22.38543791871048	28.1130355515041	27.015033786227953	22.486492743557466
220-224	19.76686779239195	27.969961366497376	27.444082707528466	24.81908813358221
225-229	21.1930372878413	29.338600222829943	26.170074228415157	23.2982882609136
230-234	20.462791740822734	28.286437759546235	27.420179132120793	23.830591367510237
235-239	21.48103775086455	28.11928636541216	25.455745105699528	24.94393077802376
240-244	21.996961409905804	31.495825555989637	23.379838231251178	23.12737480285338
245-249	20.402714473817483	30.167238210993908	24.233595230860498	25.196452084328108
250-254	20.48837377552054	28.04693898221701	25.630941528844904	25.833745713417542
255-259	20.710798570415708	29.191879729710173	26.082736467421974	24.01458523245214
260-264	20.494740345241713	30.020054694621695	25.54904429106799	23.9361606690686
265-269	19.68566508949371	29.575610250177252	25.734890249019692	25.003834411309345
270-274	18.93435198448872	31.389561719552606	24.162810551142368	25.513275744816312
275-279	19.864449942845567	30.214408704837147	23.803678140961644	26.117463211355645
280-284	17.539147169046895	31.38377392889699	25.541867430655035	25.53521147140108
285-289	19.185310587316057	29.75456873724877	26.449335127548434	24.61078554788673
290-294	18.856737711796963	32.087048371460405	24.32070148022746	24.735512436515172
295-299	17.963855247355703	32.989480690483425	22.566190620885244	26.48047344127563
300-301	14.046823226403902	36.36902953220182	26.73727771266513	22.846869528729147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.25
22	0.25
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.5
30	0.25
31	1.0
32	2.75
33	5.0
34	19.5
35	50.5
36	154.5
37	507.75
38	1904.0
39	3649.0
40	5654.0
41	7836.75
42	10986.25
43	15961.0
44	16486.5
45	13891.0
46	11093.0
47	8302.75
48	6941.25
49	4830.5
50	3216.5
51	2578.25
52	816.0
53	154.75
54	48.75
55	37.0
56	31.25
57	19.0
58	24.25
59	25.5
60	23.0
61	21.0
62	10.75
63	3.5
64	0.75
65	1.25
66	2.0
67	1.0
68	0.25
69	0.0
70	0.25
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	345555.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.06220184517824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	34.52344958461192	3.4738191811219994
2	5.402573784998331	1.087235758162436
3	4.253570992035373	1.2840086965396493
4	4.29421014983605	1.728368371730537
5	4.211077470605441	2.1186355747457295
6	4.313299443611234	2.6040773772186743
7	4.505234792432215	3.1732807288960845
8	4.060809418681045	3.2688547220455675
9	4.0948226853749246	3.7082639142411553
>10	29.4893469224089	51.11186745890406
>50	0.7061415775882351	4.555816778105336
>100	0.07875745811565102	1.2931585998032329
>500	0.017401492095846852	1.4687747352205272
>1k	0.03770323287433321	9.721695361494056
>5k	0.00870074604792305	5.864009443576429
>10k+	0.0029002486826410164	3.5381332981945173
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	12124	3.508558695431986	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	9143	2.6458884982130195	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	5726	1.6570444647017117	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5225	1.5120603087786315	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	4687	1.356368740142669	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	4410	1.276207839562443	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3956	1.144824991680051	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	3860	1.1170435965331134	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	2988	0.8646959239484309	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2568	0.7431523201805791	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	1833	0.5304510135868385	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1774	0.5133770311527832	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1678	0.48559563600584565	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1642	0.4751776128257441	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1503	0.4349524677692408	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1401	0.40543473542561964	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1013	0.2931515967067471	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	965	0.27926089913327834	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT	925	0.26768531782205435	TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	858	0.24829621912575423	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	827	0.23932514360955562	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	740	0.21414825425764347	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	718	0.20778168453647033	No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG	496	0.14353720825917726	No Hit
GGGGGGAGCAAAAGCAGAAGAAGTGTCATTCAGGGGGAGGGGAGTTTTCG	358	0.10360145273545454	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	5.787790655611987E-4	0.0	0.0
2	5.787790655611987E-4	0.0	0.0
3	5.787790655611987E-4	0.0	0.0
4	5.787790655611987E-4	0.0	0.0
5	5.787790655611987E-4	0.0	0.0
6	5.787790655611987E-4	0.0	0.0
7	8.68168598341798E-4	0.0	0.0
8	8.68168598341798E-4	0.0	0.0
9	8.68168598341798E-4	0.0	0.0
10-14	0.0011575581311223974	0.0	0.0
15-19	0.0015627034770152363	0.0	0.0
20-24	0.0018520930097958358	0.0	0.0
25-29	0.0020257267294641955	0.0	0.0
30-34	0.00428296508515287	0.0	0.0
35-39	0.005440523216275268	0.0	0.0
40-44	0.0081607848244129	0.0	0.0
45-49	0.010186511553877096	0.0	0.0
50-54	0.013601308040688168	0.0	0.0
55-59	0.01753700568650432	0.0	0.0
60-64	0.02500325563224378	0.0	0.0
65-69	0.03993575552372271	0.0	0.0
70-74	0.060482412351145265	0.0	0.0
75-79	0.09052104585377148	0.0	0.0
80-84	0.12837319674147385	0.0	0.0
85-89	0.18764017305494063	0.0	0.0
90-94	0.2666435155040442	0.0	0.0
95-99	0.36133177062985633	0.0	0.0
100-104	0.47969208953712145	0.0	0.0
105-109	0.6195251117767071	0.0	0.0
110-114	0.7895704012385872	0.0	0.0
115-119	0.983577144014701	0.0	0.0
120-124	1.2114424621261448	0.0	0.0
125-129	1.4834686229399083	0.0	0.0
130-134	1.7997713822691033	0.0	0.0
135-139	2.1704793737610513	0.0	0.0
140-144	2.570184196437615	0.0	0.0
145-149	3.022615791986804	0.0	0.0
150-154	3.5499413986196116	0.0	0.0
155-159	4.162926306955477	0.0	0.0
160-164	4.839808424129299	0.0	0.0
165-169	5.578272633878832	0.0	0.0
170-174	6.412235389445963	0.0	0.0
175-179	7.330410499052249	0.0	0.0
180-184	8.345878369579372	0.0	0.0
185-189	9.524446180781641	0.0	0.0
190-194	10.873464426791683	0.0	0.0
195-199	12.191691626513869	0.0	0.0
200-204	13.611899697587939	0.0	0.0
205-209	15.138487360912157	0.0	0.0
210-214	16.7840141222092	0.0	0.0
215-219	18.441290098537134	0.0	0.0
220-224	20.217794562370678	0.0	0.0
225-229	22.089334548769372	0.0	0.0
230-234	24.03032802303541	0.0	0.0
235-239	25.960151061336113	0.0	0.0
240-244	27.844192675550925	0.0	0.0
245-249	29.646915831054393	0.0	0.0
250-254	31.506532968702523	0.0	0.0
255-259	33.30630435097163	0.0	0.0
260-264	35.030545065184995	0.0	0.0
265-269	36.598746943323064	0.0	0.0
270-274	38.00494856101054	0.0	0.0
275-279	39.26014672049312	0.0	0.0
280-284	40.37516459029676	0.0	0.0
285-289	41.38976429222555	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	5075	0.0	294.4187	1
GGGTTAT	5110	0.0	292.40213	2
GTTATTA	5180	0.0	287.8813	4
GGGGGGA	3005	0.0	286.65558	1
GGTTATT	5245	0.0	284.59488	3
TTATTAG	5325	0.0	280.04227	5
TATTAGT	5335	0.0	279.7938	6
TTAGTAG	5340	0.0	279.53183	8
TAGTAGA	5370	0.0	277.97018	9
GGGGAGC	3100	0.0	277.87094	3
GGGAGCG	1915	0.0	266.50128	4
GGAGCGA	1925	0.0	265.11688	5
ATTAGTA	5635	0.0	264.89795	7
GCGAAAG	2015	0.0	254.00743	8
CGAAAGC	2030	0.0	252.13055	9
AAACGTT	145	0.0	244.13794	295
GAGCGAA	2095	0.0	243.60382	6
AGCGAAA	2110	0.0	241.87204	7
GGGAGCA	1480	0.0	228.22635	4
GGGGGAG	4070	0.0	212.73343	2
>>END_MODULE