FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_30301_flu.3510000003b9e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_30301_flu.3510000003b9e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345555
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC121243.508558695431986No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT91432.6458884982130195No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG57261.6570444647017117No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA52251.5120603087786315No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG46871.356368740142669No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA44101.276207839562443No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT39561.144824991680051No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA38601.1170435965331134No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT29880.8646959239484309No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG25680.7431523201805791No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA18330.5304510135868385No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT17740.5133770311527832No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA16780.48559563600584565No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG16420.4751776128257441No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT15030.4349524677692408No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT14010.40543473542561964No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG10130.2931515967067471No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT9650.27926089913327834No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT9250.26768531782205435TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT8580.24829621912575423No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT8270.23932514360955562No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA7400.21414825425764347No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA7180.20778168453647033No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG4960.14353720825917726No Hit
GGGGGGAGCAAAAGCAGAAGAAGTGTCATTCAGGGGGAGGGGAGTTTTCG3580.10360145273545454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA50750.0294.41871
GGGTTAT51100.0292.402132
GTTATTA51800.0287.88134
GGGGGGA30050.0286.655581
GGTTATT52450.0284.594883
TTATTAG53250.0280.042275
TATTAGT53350.0279.79386
TTAGTAG53400.0279.531838
TAGTAGA53700.0277.970189
GGGGAGC31000.0277.870943
GGGAGCG19150.0266.501284
GGAGCGA19250.0265.116885
ATTAGTA56350.0264.897957
GCGAAAG20150.0254.007438
CGAAAGC20300.0252.130559
AAACGTT1450.0244.13794295
GAGCGAA20950.0243.603826
AGCGAAA21100.0241.872047
GGGAGCA14800.0228.226354
GGGGGAG40700.0212.733432
Produced by FastQC (version 0.11.2)