FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_30295_flu.3510000003b97a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_30295_flu.3510000003b97a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320926
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC117793.6703165215657187No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT76942.3974374154789575No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG69942.1793185968104796No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA47341.475106410823679No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA44671.3919096614172737No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG42901.3367567601253871No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA40141.25075562590753No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT33111.0317020123019014No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT30470.9494400578326468No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG27040.8425618366850925No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT23350.7275820594155662No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA20850.6496824813196812No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG19290.6010731445878489No Hit
GGGGGGAGCGAAAGCAGGTACTGATACAAAATGGAAGATTTTGTGCGACA17450.5437390551092774No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT16910.5269127462405664No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT13060.40694739597290336No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT10710.33372179256277146No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG10090.314402697194992No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT8480.264235368901242No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA7850.244604675221079No Hit
GGGGGGAGCAAAAGCAGGTACTGATACAAAATGGAAGATTTTGTGCGACA7330.2284015629771349No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6440.20066931317499984No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT6430.20035771486261628TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG4770.14863239500694866No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC3900.12152334182958065No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTGG108.462189E-4295.0295
CGGGTTA43050.0292.944241
GGGTTAT43300.0292.274842
GTTATTA44300.0285.677224
GGGGGGA35450.0285.430181
GGTTATT44500.0284.393253
TTATTAG45550.0277.837555
TTAGTAG45700.0276.92568
GGGGAGC36400.0275.954683
TAGTAGA46200.0273.92869
GGGAGCG25200.0270.41674
GGAGCGA25250.0269.88125
TATTAGT47150.0268.722176
ATTAGTA47950.0264.238777
CGAAAGC26400.0257.566289
AGCGAAA28350.0239.850077
GAGCGAA28500.0239.105276
GCGAAAG28800.0237.126748
GGGGGAG43850.0230.079822
GGGAGCA13750.0224.24
Produced by FastQC (version 0.11.2)