##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_30295_flu.3510000003b97a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	320926
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.76952319226239	34.0	34.0	34.0	34.0	34.0
2	33.84525716208721	34.0	34.0	34.0	34.0	34.0
3	33.868212609760505	34.0	34.0	34.0	34.0	34.0
4	33.889008681128985	34.0	34.0	34.0	34.0	34.0
5	33.88152720564865	34.0	34.0	34.0	34.0	34.0
6	37.648735222450036	38.0	38.0	38.0	38.0	38.0
7	37.66189090319887	38.0	38.0	38.0	38.0	38.0
8	37.653072670958416	38.0	38.0	38.0	38.0	38.0
9	37.573359590684454	38.0	38.0	38.0	37.0	38.0
10-14	37.65879299277715	38.0	38.0	38.0	37.8	38.0
15-19	37.626745729545135	38.0	38.0	38.0	37.2	38.0
20-24	37.6259349507363	38.0	38.0	38.0	37.0	38.0
25-29	37.527028660812775	38.0	38.0	38.0	37.0	38.0
30-34	37.45182440811901	38.0	38.0	38.0	37.0	38.0
35-39	37.35720446458062	38.0	38.0	38.0	37.0	38.0
40-44	37.36931566778634	38.0	38.0	38.0	37.0	38.0
45-49	37.33726217258807	38.0	38.0	38.0	37.0	38.0
50-54	37.2951714725513	38.0	38.0	38.0	37.0	38.0
55-59	37.25307267095842	38.0	38.0	38.0	37.0	38.0
60-64	37.08303970385696	38.0	38.0	38.0	36.6	38.0
65-69	37.00915787440095	38.0	38.0	38.0	36.4	38.0
70-74	37.09936246985286	38.0	38.0	38.0	37.0	38.0
75-79	37.02999320715679	38.0	38.0	38.0	36.8	38.0
80-84	36.94281298492488	38.0	38.0	38.0	36.2	38.0
85-89	36.975735839414696	38.0	38.0	38.0	36.0	38.0
90-94	36.82976574038875	38.0	38.0	38.0	35.8	38.0
95-99	36.83553280195434	38.0	38.0	38.0	36.0	38.0
100-104	36.81886603142158	38.0	38.0	38.0	36.0	38.0
105-109	36.66883767597515	38.0	38.0	38.0	35.0	38.0
110-114	36.57765715460885	38.0	38.0	38.0	34.8	38.0
115-119	36.68123368003839	38.0	38.0	38.0	35.0	38.0
120-124	36.590837763222666	38.0	37.8	38.0	34.8	38.0
125-129	36.50505599421673	38.0	37.0	38.0	34.0	38.0
130-134	36.390831531256424	38.0	37.0	38.0	34.0	38.0
135-139	36.15176707402953	38.0	37.0	38.0	33.6	38.0
140-144	36.12287817129183	38.0	37.0	38.0	33.4	38.0
145-149	35.701065043031726	38.0	37.0	38.0	30.4	38.0
150-154	35.3416663031353	38.0	36.4	38.0	28.6	38.0
155-159	35.455401556745166	38.0	36.2	38.0	29.2	38.0
160-164	35.414221346977186	38.0	36.0	38.0	28.8	38.0
165-169	35.20977982463247	38.0	36.0	38.0	28.2	38.0
170-174	35.08070957167696	38.0	35.4	38.0	28.0	38.0
175-179	34.73792151461708	38.0	35.2	38.0	25.4	38.0
180-184	34.440229211718595	37.6	35.0	38.0	23.8	38.0
185-189	33.68601359815035	37.0	33.8	37.8	22.8	38.0
190-194	32.716279765428794	36.8	31.2	37.0	21.2	38.0
195-199	32.49543882390333	36.8	30.8	37.2	20.4	37.8
200-204	32.31580488960071	37.0	30.2	37.0	20.0	37.4
205-209	31.177799866635922	35.4	28.2	37.0	18.2	37.2
210-214	30.476139670827543	34.6	26.6	37.0	16.0	37.0
215-219	29.63599583704655	33.4	24.8	37.0	14.4	37.0
220-224	28.139619725419568	31.6	22.2	36.2	9.6	37.0
225-229	27.099611125306144	30.2	20.0	36.2	9.0	37.0
230-234	26.670055402179948	29.4	20.0	35.8	9.0	37.0
235-239	25.642215339361723	28.2	18.4	34.6	8.8	37.0
240-244	24.510498993537453	26.4	16.6	33.8	8.0	37.0
245-249	23.622954824476672	25.0	15.0	33.0	8.0	36.4
250-254	22.721326411696158	23.6	13.8	31.8	8.0	36.0
255-259	22.099662227429373	22.4	13.2	31.0	8.0	36.0
260-264	20.78924736543627	20.2	11.4	29.2	7.8	35.2
265-269	19.966851548332013	19.6	9.8	28.4	7.0	34.0
270-274	18.977631603547234	18.4	8.8	26.8	7.0	33.4
275-279	17.663371618379315	16.2	8.0	24.8	7.0	31.4
280-284	17.3910421717156	16.2	8.0	24.4	7.0	30.8
285-289	16.293763048179333	14.2	8.0	22.4	7.0	29.4
290-294	15.580920835332757	13.0	8.0	21.0	7.0	28.4
295-299	14.776517327982152	13.0	8.0	20.0	7.0	27.0
300-301	13.014456603703035	10.0	8.0	16.0	7.0	22.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.10086405857175151
1101	2	0.041297569135032575
1101	3	0.0819273552043498
1101	4	0.03457743264367963
1101	5	0.03305318151197412
1101	6	0.14839553259587746
1101	7	0.1061223562797835
1101	8	0.10641404798246157
1101	9	0.07463717245544643
1101	10-14	0.08018380943936165
1101	15-19	0.07544145247077694
1101	20-24	0.10209342508134256
1101	25-29	0.12536168586836993
1101	30-34	0.17188298410691516
1101	35-39	0.16862229040321353
1101	40-44	0.1724968482404421
1101	45-49	0.10395812384088998
1101	50-54	0.11133769903208446
1101	55-59	0.08295105721474982
1101	60-64	0.3182287757804687
1101	65-69	0.27787749027369557
1101	70-74	0.1805294175453085
1101	75-79	0.16486044365332475
1101	80-84	0.23328464742250077
1101	85-89	0.17016586501246422
1101	90-94	0.3475680281379425
1101	95-99	0.06451725143638498
1101	100-104	0.2691435232345327
1101	105-109	0.19523975892634127
1101	110-114	0.2750152832609487
1101	115-119	0.17147886950032643
1101	120-124	0.22975015678298405
1101	125-129	0.3035411003378954
1101	130-134	0.2666748423508096
1101	135-139	0.29204082206757676
1101	140-144	0.12741983003891022
1101	145-149	0.4623164950910805
1101	150-154	0.6189108871280098
1101	155-159	0.3806274539879553
1101	160-164	0.3461961458340639
1101	165-169	0.283028685941197
1101	170-174	0.2936634611059219
1101	175-179	0.35630958240262345
1101	180-184	0.20762255882858938
1101	185-189	0.417214551450634
1101	190-194	0.3701274291533423
1101	195-199	0.29566175530668914
1101	200-204	-0.15457573488060206
1101	205-209	-0.15359367530797385
1101	210-214	-0.34393181247148163
1101	215-219	-0.0749402195213662
1101	220-224	0.6447745232940285
1101	225-229	0.43791001818221886
1101	230-234	0.3120323032792349
1101	235-239	0.5412328202795855
1101	240-244	0.8069093286764506
1101	245-249	0.9006121082014502
1101	250-254	0.1485142307527596
1101	255-259	-0.24092716793945357
1101	260-264	0.48741483997288526
1101	265-269	-0.17460479773170334
1101	270-274	-0.0955441948625122
1101	275-279	0.21979214570765748
1101	280-284	-0.046058730781112445
1101	285-289	-0.20346956936807814
1101	290-294	-0.16080444494912705
1101	295-299	-0.1811212170296681
1101	300-301	0.0033519278795726137
1102	1	-0.029942704958465072
1102	2	0.04113797883640302
1102	3	0.07648403204549226
1102	4	0.03608419510286609
1102	5	0.058570376288336945
1102	6	0.13615740086630268
1102	7	0.19256853912425953
1102	8	0.04921314680048994
1102	9	0.10178765172955195
1102	10-14	0.07938729569663394
1102	15-19	0.07662040783005608
1102	20-24	0.03834932165533189
1102	25-29	0.035351319687705995
1102	30-34	0.024821242789862197
1102	35-39	0.08664636122104952
1102	40-44	0.035158900686695915
1102	45-49	0.12105038859974826
1102	50-54	0.08404143186354673
1102	55-59	-9.405306512064726E-4
1102	60-64	0.19541843375401413
1102	65-69	0.27396105808016813
1102	70-74	0.23759286978538796
1102	75-79	0.09198691249511626
1102	80-84	0.1596899406445047
1102	85-89	0.27438695652383416
1102	90-94	0.2796256934325214
1102	95-99	0.10865647743782603
1102	100-104	0.20583247024049456
1102	105-109	0.13634288732752253
1102	110-114	0.37279870341045296
1102	115-119	0.13654325915369725
1102	120-124	0.1991162957473236
1102	125-129	0.1949532731957646
1102	130-134	0.2847232111510536
1102	135-139	0.2506114680919751
1102	140-144	0.25553805913294525
1102	145-149	0.3285171389157213
1102	150-154	0.9083205611756142
1102	155-159	0.5726660547117248
1102	160-164	0.5859217655404478
1102	165-169	0.6566842095627194
1102	170-174	0.5102864441217889
1102	175-179	0.7059773901638948
1102	180-184	0.3704178907404696
1102	185-189	0.6939915750484289
1102	190-194	0.9088119737674489
1102	195-199	0.7335579813555597
1102	200-204	0.18755624773412904
1102	205-209	0.24104064005683057
1102	210-214	-0.17503840585121822
1102	215-219	0.05963523434050444
1102	220-224	0.831814640844506
1102	225-229	0.9961430804037441
1102	230-234	0.6901807970199876
1102	235-239	1.182711057770078
1102	240-244	1.1902785818082329
1102	245-249	1.7020754505974018
1102	250-254	0.6057004668802612
1102	255-259	0.5646467029591804
1102	260-264	1.1496283142824417
1102	265-269	1.1146562129205897
1102	270-274	1.363434628511282
1102	275-279	1.2253760808593661
1102	280-284	0.9828874987902481
1102	285-289	0.7995068615889913
1102	290-294	0.771221589835811
1102	295-299	0.934220874120836
1102	300-301	0.6221625919621214
1103	1	-0.106608965331219
1103	2	-0.015042662239352467
1103	3	-0.022454139828035125
1103	4	-0.03551319845163192
1103	5	0.01663243838405748
1103	6	0.020311798157308658
1103	7	0.052858072572753656
1103	8	0.052201508581397604
1103	9	-0.0023861075679221244
1103	10-14	0.030070780129264563
1103	15-19	-0.029494875966243228
1103	20-24	-0.006784047824154982
1103	25-29	-0.017308574479734773
1103	30-34	0.04669274463429218
1103	35-39	-0.5058635404418226
1103	40-44	-0.29877266540821523
1103	45-49	-0.22729956775867066
1103	50-54	-0.049100242823925555
1103	55-59	-0.3537022784995969
1103	60-64	0.11133156531339239
1103	65-69	0.2269399630200013
1103	70-74	-0.001064454923302094
1103	75-79	0.2606406365991276
1103	80-84	0.17254367903886703
1103	85-89	0.1610155436437637
1103	90-94	0.3449120816057203
1103	95-99	0.29060189675415415
1103	100-104	0.1514044970446946
1103	105-109	0.07298205209930586
1103	110-114	0.1816152813229195
1103	115-119	0.04961541555162796
1103	120-124	0.1954064120174266
1103	125-129	0.24626174974769555
1103	130-134	0.3340887621906745
1103	135-139	0.3891823502681504
1103	140-144	0.4692293233935132
1103	145-149	0.5874538537352265
1103	150-154	0.8571160398248594
1103	155-159	-0.21310076900855535
1103	160-164	-0.752446810287303
1103	165-169	0.38607985437217707
1103	170-174	0.7978713630521383
1103	175-179	0.5334476221296924
1103	180-184	0.43857943880433936
1103	185-189	0.8374399455996837
1103	190-194	0.5145956376699203
1103	195-199	0.6079554404774967
1103	200-204	0.3173635103078709
1103	205-209	0.5444155414605127
1103	210-214	0.6329000168037275
1103	215-219	0.5799741675247674
1103	220-224	1.2023078956650473
1103	225-229	2.0795064654765056
1103	230-234	1.0369251919152553
1103	235-239	1.4244755502039048
1103	240-244	1.3216261186163578
1103	245-249	1.3040629283388014
1103	250-254	1.4550691001495721
1103	255-259	0.5038746604636444
1103	260-264	1.5618109672135674
1103	265-269	0.4937410428865867
1103	270-274	0.7278175030827505
1103	275-279	1.579439090040207
1103	280-284	0.9277018574973397
1103	285-289	1.0863464413409822
1103	290-294	1.0354451691731015
1103	295-299	1.1078905067330638
1103	300-301	-0.9362379549935458
2116	1	0.058235536863378456
2116	2	-0.01293082741214846
2116	3	-0.009338969653605034
2116	4	0.03246967513346277
2116	5	-0.027215377087649983
2116	6	-0.43635075794908573
2116	7	-0.17333033454375624
2116	8	3.8775834345727844E-4
2116	9	0.0735061460950277
2116	10-14	0.05215304099259299
2116	15-19	0.05917873727141654
2116	20-24	-0.16411422512999962
2116	25-29	-0.21610437343195343
2116	30-34	-0.0146083994256756
2116	35-39	-0.12041878609607437
2116	40-44	-0.06361247770182388
2116	45-49	0.135892985782057
2116	50-54	0.12821249908530774
2116	55-59	0.21504587657611296
2116	60-64	0.3306550608897041
2116	65-69	0.23936682125532371
2116	70-74	-0.6285105831341582
2116	75-79	0.1927270080227359
2116	80-84	-0.08367140533797368
2116	85-89	0.18534426708030338
2116	90-94	0.3310564588282503
2116	95-99	0.21482909762813307
2116	100-104	0.1285086762982175
2116	105-109	0.17912058073944337
2116	110-114	0.33589564360396906
2116	115-119	0.3547668579720593
2116	120-124	-0.13265050682797863
2116	125-129	0.04304916757098454
2116	130-134	0.23930117332395184
2116	135-139	0.3888620576183186
2116	140-144	0.42964904194428044
2116	145-149	0.6872047372298127
2116	150-154	0.9265163670144219
2116	155-159	-0.3112965365108522
2116	160-164	-0.47565630264691094
2116	165-169	0.6782446850805428
2116	170-174	0.5961882403505285
2116	175-179	0.6394421432218351
2116	180-184	0.5857137713624567
2116	185-189	1.0427080837917586
2116	190-194	0.8167891319496192
2116	195-199	0.9381501265437961
2116	200-204	0.61180404650149
2116	205-209	0.603810654583679
2116	210-214	0.3746147549555232
2116	215-219	0.8924126125743044
2116	220-224	1.593970631091178
2116	225-229	1.083096998497549
2116	230-234	1.308415283809726
2116	235-239	1.324682181723901
2116	240-244	1.606548362816742
2116	245-249	1.0221038739331902
2116	250-254	1.3600792937873045
2116	255-259	1.1063100984590086
2116	260-264	0.36497720213346696
2116	265-269	0.7468450676492644
2116	270-274	1.192165979278741
2116	275-279	1.888760406191949
2116	280-284	0.35097342207589577
2116	285-289	-1.140999543689956
2116	290-294	0.35879784145109994
2116	295-299	0.8270906855010587
2116	300-301	1.3133302982390394
1117	1	-0.08667702165088542
1117	2	0.014176727698909986
1117	3	-0.006002441476468334
1117	4	0.031304251677759964
1117	5	-0.015655598782025493
1117	6	-0.480338200823887
1117	7	-0.10821941790139533
1117	8	-0.06917510368096202
1117	9	0.13848232367278257
1117	10-14	0.14317386303979163
1117	15-19	0.09185809996953509
1117	20-24	0.05384802015066015
1117	25-29	0.1370456036485166
1117	30-34	0.15417853227412337
1117	35-39	-0.05966674764682267
1117	40-44	-0.004526480841100522
1117	45-49	0.21367604199742374
1117	50-54	0.2044906449646433
1117	55-59	0.28317772639067584
1117	60-64	0.27160379059203876
1117	65-69	0.41284211677838556
1117	70-74	0.01265928532161098
1117	75-79	0.14888319421267937
1117	80-84	0.05299928961000688
1117	85-89	0.07140696936252766
1117	90-94	0.2426776336973333
1117	95-99	0.27309471416354114
1117	100-104	0.3099105181951245
1117	105-109	0.3536968472171935
1117	110-114	0.2729802791834217
1117	115-119	0.23208541168016694
1117	120-124	0.35900483897913205
1117	125-129	0.39190471895934564
1117	130-134	0.29912003957112177
1117	135-139	0.4181132140128696
1117	140-144	0.3617402755707033
1117	145-149	0.8256281512676722
1117	150-154	1.0973752243715893
1117	155-159	-0.07317371560268526
1117	160-164	-0.6132565582344114
1117	165-169	0.45610858473467175
1117	170-174	0.6282497480389893
1117	175-179	0.7854565061283125
1117	180-184	0.5727082567842459
1117	185-189	1.019884675310145
1117	190-194	1.2543482712864815
1117	195-199	1.042719586615199
1117	200-204	1.1497004502186243
1117	205-209	1.6140285984933378
1117	210-214	1.1730503927435798
1117	215-219	0.8874681056220446
1117	220-224	1.6400844508742018
1117	225-229	1.0436640138189048
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2108	170-174	-0.91219792286239
2108	175-179	-0.8580482327303329
2108	180-184	-0.4736276980453553
2108	185-189	-1.1646121990596967
2108	190-194	-0.8114190205062144
2108	195-199	-1.6015675680530883
2108	200-204	-1.0766274384686128
2108	205-209	-0.677284166176964
2108	210-214	-0.08289637071679223
2108	215-219	-0.8558430458197783
2108	220-224	-0.40223053198524283
2108	225-229	-0.1035188859592715
2108	230-234	-0.39670771288669826
2108	235-239	-0.7568532975673321
2108	240-244	-0.6178500318101676
2108	245-249	-0.3489748384920688
2108	250-254	-0.7159029051369892
2108	255-259	-0.9563343987698119
2108	260-264	-0.09353045615140232
2108	265-269	-1.0152621210366775
2108	270-274	-1.0336242847341417
2108	275-279	-0.4842655036559762
2108	280-284	-0.7066207024770321
2108	285-289	0.05438200544046623
2108	290-294	-0.5544983608040717
2108	295-299	0.10368609326036093
2108	300-301	-0.04375327398909867
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	28.0
13	137.0
14	381.0
15	506.0
16	459.0
17	478.0
18	790.0
19	768.0
20	771.0
21	1014.0
22	1550.0
23	2246.0
24	3468.0
25	4971.0
26	7126.0
27	9805.0
28	13423.0
29	19181.0
30	27155.0
31	38286.0
32	50780.0
33	60429.0
34	52614.0
35	22578.0
36	1977.0
37	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.647644628356694	23.71917513694746	15.665916753394862	30.967263481300982
2	41.98039423418483	24.33395860728018	18.51672971339187	15.168917445143117
3	41.38742264571895	23.040202414263725	20.003676860086124	15.568698079931199
4	41.82210229149399	22.716140169384843	19.83510217308663	15.626655366034537
5	28.39564260919963	22.933012594803788	33.43854969681484	15.232795099181743
6	27.03582757395786	22.86383776945464	33.848924674223966	16.251409982363533
7	17.691928980512643	45.54819491097636	20.847485090020754	15.912391018490244
8	26.51483519565258	21.791939574855263	34.887793447710685	16.80543178178147
9	16.281323420352358	20.223042072004137	36.40745841720521	27.088176090438292
10-14	25.697824420582936	33.85397256688458	23.78616877410992	16.662034238422567
15-19	21.686058468307333	34.287218860422655	22.535849385839725	21.490873285430286
20-24	24.60155923795517	30.81501654587039	26.644023855966797	17.93940036020765
25-29	19.75844898824028	26.24891719586447	35.890579136623394	18.102054679271856
30-34	19.97426197939712	28.355259467914724	30.69480191695282	20.97567663573534
35-39	20.85988670285362	29.560210141901873	27.803543496008427	21.776359659236086
40-44	20.427949122227552	29.038407607984396	29.064145628587273	21.469497641200775
45-49	21.41602737079576	29.930201978026087	26.414001981765267	22.23976866941289
50-54	21.9483619276718	29.47071910658532	27.758112462062908	20.822806503679974
55-59	21.67403077344933	27.11135900487963	29.84532259773281	21.36928762393823
60-64	22.10796258327465	26.062269806746723	29.385777406629565	22.44399020334906
65-69	20.457364002916563	27.1914397711622	28.892828876438802	23.458367349482433
70-74	21.106921844911287	26.373681160142837	29.36714382754903	23.152253167396843
75-79	20.459482871440766	26.098041293008357	30.462910452876983	22.97956538267389
80-84	22.991780036519323	26.085826639162924	27.704455232670462	23.217938091647294
85-89	21.334263973626317	27.60031907667188	26.35729108891146	24.70812586079034
90-94	20.360332288440325	29.796463982351074	28.748870456117608	21.094333273090992
95-99	22.36390943706649	29.61511376454385	26.887506777263294	21.133470021126367
100-104	22.54351470432436	28.32191844848968	26.93518131905798	22.19938552812798
105-109	23.376479312988042	27.60418289574544	24.560677539370445	24.458660251896074
110-114	21.67720907623564	28.177897708506013	29.028311822663166	21.116581392595176
115-119	23.335472975078368	28.02265942927653	27.044739285692028	21.597128309953074
120-124	19.22935505381303	28.505512174146062	28.042040844306786	24.22309192773412
125-129	20.89179437004169	25.360176489284136	30.56978867402454	23.178240466649633
130-134	24.556190523672125	28.70668004462088	26.38327838816425	20.353851043542747
135-139	23.779812168537294	27.740912235219334	25.219334052086772	23.259941544156597
140-144	21.12698877622879	29.166536834036506	26.854726634800546	22.85174775493416
145-149	21.007521983260936	27.193247041374025	29.029308937262794	22.76992203810224
150-154	22.998323601079377	26.061023413497193	26.088506384649417	24.85214660077401
155-159	21.832571994790076	27.114225709353555	29.549304200968447	21.50389809488792
160-164	24.16663031353022	29.433825866399104	26.20610358774297	20.193440232327703
165-169	20.335404423449642	26.223927011211305	28.24532758330581	25.195340982033244
170-174	22.096495765378936	29.366520630924263	26.11349656930258	22.42348703439422
175-179	20.14121635517222	29.526245925852066	28.30565301658326	22.02688470239245
180-184	19.89131450864062	29.764307036513088	26.737378710356907	23.606999744489386
185-189	21.672846699862276	28.06491216043574	27.68688108785203	22.57536005184996
190-194	20.122271177779304	28.3462231168556	26.386519010613043	25.144986694752063
195-199	20.78603790281872	30.27333403962284	27.157288596124964	21.783339461433478
200-204	21.65452471909412	28.475848014807152	26.91330711752865	22.956320148570075
205-209	20.971189620037016	28.275677258931964	27.640889176944217	23.1122439440868
210-214	22.11936708150789	28.528009572300157	27.006537332593805	22.34608601359815
215-219	22.262079108579545	27.82672641044976	27.440095224444267	22.47109925652643
220-224	19.13749587132236	27.94201778603167	27.809401544281236	25.11108479836473
225-229	20.91285841595882	29.208789565195715	26.280824863052544	23.597527155792925
230-234	20.93342390457613	27.524102129462868	27.680898400254268	23.861575565706737
235-239	21.098259411827026	28.19322834547528	25.351825654512254	25.35668658818544
240-244	21.84628232053495	31.691667237930236	23.45082667032275	23.011223771212055
245-249	19.92920486342646	30.52566635299103	24.187569720122397	25.357559063460112
250-254	20.630488025276854	28.12149841396459	25.281217476926145	25.96679608383241
255-259	20.500800807662824	29.041211992795844	26.37891601179088	24.079071187750447
260-264	19.968466250786783	30.272212285698263	25.817353533213268	23.94196793030169
265-269	19.23783052790986	29.793161040239806	26.039024572642916	24.929983859207418
270-274	18.270130808971537	31.698647040127632	24.4521790069985	25.57904314390233
275-279	19.22592747237681	30.455369773717305	24.014632656749534	26.30407009715635
280-284	16.723169827312216	31.687180222231916	25.832933448832442	25.756716501623426
285-289	18.838797729071498	29.867446077912042	26.889376367137597	24.404379825878863
290-294	18.175965798969234	32.42255223945707	24.754055452035672	24.647426509538025
295-299	17.36119853174875	33.34600499803693	22.64353776259948	26.649258707614838
300-301	13.425680686513402	36.70768339118675	26.495827698597186	23.37080822370266
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.25
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.5
31	1.75
32	4.75
33	9.0
34	30.75
35	94.75
36	248.25
37	758.25
38	1769.5
39	2729.25
40	4821.5
41	7137.75
42	10420.5
43	13953.25
44	16812.5
45	13600.0
46	9957.0
47	7640.75
48	6416.5
49	4405.0
50	3723.75
51	1446.75
52	278.5
53	100.25
54	71.25
55	53.75
56	46.5
57	48.0
58	47.75
59	54.25
60	54.0
61	32.0
62	16.5
63	6.0
64	3.25
65	2.25
66	1.75
67	0.0
68	0.5
69	0.5
70	0.75
71	1.25
72	0.25
73	0.25
74	0.5
75	0.0
76	0.0
77	0.0
78	0.25
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.25
91	0.25
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	320926.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.19770603605091
#Duplication Level	Percentage of deduplicated	Percentage of total
1	36.44238401863939	4.0807110349360345
2	6.504008573245526	1.456599521183166
3	4.137986829178014	1.3900788028255746
4	5.04868554353755	2.261347863399736
5	5.420884028494203	3.0350732903300752
6	4.865119073178159	3.268690392710009
7	4.75104152508673	3.724053645409454
8	4.009988980525174	3.59221422493795
9	3.6299977342843666	3.6582882786042465
>10	24.554773349446073	46.78290744178447
>50	0.49624619652150415	3.5687616890000196
>100	0.07512345773340354	1.4957267282997813
>500	0.013861019593459381	1.1339761159393258
>1k	0.041583058780369046	12.335598931067135
>5k	0.00554440783738254	4.559496630417084
>10k+	0.00277220391869127	3.656475409155967
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	11779	3.6703165215657187	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	7694	2.3974374154789575	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	6994	2.1793185968104796	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4734	1.475106410823679	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	4467	1.3919096614172737	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	4290	1.3367567601253871	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	4014	1.25075562590753	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3311	1.0317020123019014	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3047	0.9494400578326468	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2704	0.8425618366850925	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2335	0.7275820594155662	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	2085	0.6496824813196812	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1929	0.6010731445878489	No Hit
GGGGGGAGCGAAAGCAGGTACTGATACAAAATGGAAGATTTTGTGCGACA	1745	0.5437390551092774	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1691	0.5269127462405664	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1306	0.40694739597290336	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1071	0.33372179256277146	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1009	0.314402697194992	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	848	0.264235368901242	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	785	0.244604675221079	No Hit
GGGGGGAGCAAAAGCAGGTACTGATACAAAATGGAAGATTTTGTGCGACA	733	0.2284015629771349	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	644	0.20066931317499984	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT	643	0.20035771486261628	TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG	477	0.14863239500694866	No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC	390	0.12152334182958065	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	3.115983123835401E-4	0.0	0.0
8	6.231966247670802E-4	0.0	0.0
9	9.347949371506204E-4	0.0	0.0
10-14	0.0013087129120108684	0.0	0.0
15-19	0.0019319095367779488	0.0	0.0
20-24	0.002492786499068321	0.0	0.0
25-29	0.002492786499068321	0.0	0.0
30-34	0.003801499411079189	0.0	0.0
35-39	0.0051102123230900584	0.0	0.0
40-44	0.006418925235100926	0.0	0.0
45-49	0.008787072409215831	0.0	0.0
50-54	0.012152334182958066	0.0	0.0
55-59	0.016327751568897507	0.0	0.0
60-64	0.02343219309124222	0.0	0.0
65-69	0.03240622448788817	0.0	0.0
70-74	0.04692670584496114	0.0	0.0
75-79	0.0721661691480279	0.0	0.0
80-84	0.10656662283517074	0.0	0.0
85-89	0.1514367798184005	0.0	0.0
90-94	0.21120133613356348	0.0	0.0
95-99	0.28286894798177775	0.0	0.0
100-104	0.38096009672011616	0.0	0.0
105-109	0.4982457015012806	0.0	0.0
110-114	0.6431389167596269	0.0	0.0
115-119	0.8223702660426391	0.0	0.0
120-124	1.0330107252139122	0.0	0.0
125-129	1.2850314402697194	0.0	0.0
130-134	1.5855991723948824	0.0	0.0
135-139	1.9197572025949907	0.0	0.0
140-144	2.3096290110492763	0.0	0.0
145-149	2.7433115422246876	0.0	0.0
150-154	3.251839988034625	0.0	0.0
155-159	3.8531624112723803	0.0	0.0
160-164	4.506023195378374	0.0	0.0
165-169	5.2460068676268055	0.0	0.0
170-174	6.100721038494855	0.0	0.0
175-179	7.010027233692502	0.0	0.0
180-184	8.005334563108006	0.0	0.0
185-189	9.178128291257172	0.0	0.0
190-194	10.51058499467167	0.0	0.0
195-199	11.825966110567546	0.0	0.0
200-204	13.229716507855395	0.0	0.0
205-209	14.705633074291267	0.0	0.0
210-214	16.227977789272295	0.0	0.0
215-219	17.802234783096416	0.0	0.0
220-224	19.486735259841833	0.0	0.0
225-229	21.264652910639835	0.0	0.0
230-234	23.076410138162693	0.0	0.0
235-239	24.865919246181363	0.0	0.0
240-244	26.550045804951925	0.0	0.0
245-249	28.195721131974352	0.0	0.0
250-254	29.902033490586614	0.0	0.0
255-259	31.500595152776658	0.0	0.0
260-264	33.06874481967806	0.0	0.0
265-269	34.52085527504783	0.0	0.0
270-274	35.873752827754686	0.0	0.0
275-279	37.05576986595041	0.0	0.0
280-284	38.13464786274717	0.0	0.0
285-289	39.11076073611984	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTGG	10	8.462189E-4	295.0	295
CGGGTTA	4305	0.0	292.94424	1
GGGTTAT	4330	0.0	292.27484	2
GTTATTA	4430	0.0	285.67722	4
GGGGGGA	3545	0.0	285.43018	1
GGTTATT	4450	0.0	284.39325	3
TTATTAG	4555	0.0	277.83755	5
TTAGTAG	4570	0.0	276.9256	8
GGGGAGC	3640	0.0	275.95468	3
TAGTAGA	4620	0.0	273.9286	9
GGGAGCG	2520	0.0	270.4167	4
GGAGCGA	2525	0.0	269.8812	5
TATTAGT	4715	0.0	268.72217	6
ATTAGTA	4795	0.0	264.23877	7
CGAAAGC	2640	0.0	257.56628	9
AGCGAAA	2835	0.0	239.85007	7
GAGCGAA	2850	0.0	239.10527	6
GCGAAAG	2880	0.0	237.12674	8
GGGGGAG	4385	0.0	230.07982	2
GGGAGCA	1375	0.0	224.2	4
>>END_MODULE