##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_30292_flu.3510000003b9a1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	297430
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.79579733046431	34.0	34.0	34.0	34.0	34.0
2	33.88048280267626	34.0	34.0	34.0	34.0	34.0
3	33.8888007262213	34.0	34.0	34.0	34.0	34.0
4	33.89428773156709	34.0	34.0	34.0	34.0	34.0
5	33.89012204552331	34.0	34.0	34.0	34.0	34.0
6	37.64915106075379	38.0	38.0	38.0	38.0	38.0
7	37.64733550751437	38.0	38.0	38.0	38.0	38.0
8	37.65613085431866	38.0	38.0	38.0	38.0	38.0
9	37.57168745587197	38.0	38.0	38.0	37.0	38.0
10-14	37.659884342534376	38.0	38.0	38.0	38.0	38.0
15-19	37.63224826009481	38.0	38.0	38.0	37.6	38.0
20-24	37.62696567259523	38.0	38.0	38.0	37.2	38.0
25-29	37.5406852032411	38.0	38.0	38.0	37.0	38.0
30-34	37.480582321890864	38.0	38.0	38.0	37.0	38.0
35-39	37.39612480247454	38.0	38.0	38.0	37.0	38.0
40-44	37.406336953232696	38.0	38.0	38.0	37.0	38.0
45-49	37.37790068251353	38.0	38.0	38.0	37.0	38.0
50-54	37.34019231415795	38.0	38.0	38.0	37.0	38.0
55-59	37.29241502202199	38.0	38.0	38.0	37.0	38.0
60-64	37.125776149009845	38.0	38.0	38.0	36.6	38.0
65-69	37.052162189422724	38.0	38.0	38.0	36.4	38.0
70-74	37.13000033621356	38.0	38.0	38.0	37.0	38.0
75-79	37.057840836499345	38.0	38.0	38.0	36.8	38.0
80-84	36.962526981138424	38.0	38.0	38.0	36.0	38.0
85-89	37.001767138486365	38.0	38.0	38.0	36.0	38.0
90-94	36.85402750226944	38.0	38.0	38.0	35.8	38.0
95-99	36.854751706283835	38.0	38.0	38.0	36.0	38.0
100-104	36.835910970648555	38.0	38.0	38.0	36.0	38.0
105-109	36.69060753790808	38.0	38.0	38.0	35.0	38.0
110-114	36.59014490804559	38.0	38.0	38.0	34.6	38.0
115-119	36.68644050701005	38.0	38.0	38.0	35.0	38.0
120-124	36.60280805567697	38.0	37.8	38.0	34.8	38.0
125-129	36.51290320411526	38.0	37.0	38.0	34.0	38.0
130-134	36.382924385569716	38.0	37.0	38.0	34.0	38.0
135-139	36.137704333792826	38.0	37.0	38.0	33.4	38.0
140-144	36.10677470329153	38.0	37.0	38.0	33.4	38.0
145-149	35.697117304912084	38.0	37.0	38.0	30.4	38.0
150-154	35.31305718992704	38.0	36.2	38.0	28.0	38.0
155-159	35.409943852335005	38.0	36.0	38.0	29.0	38.0
160-164	35.353720203072996	38.0	36.0	38.0	28.6	38.0
165-169	35.1621786638873	38.0	35.6	38.0	28.0	38.0
170-174	35.00763137544969	38.0	35.2	38.0	27.8	38.0
175-179	34.659884342534376	38.0	35.0	38.0	25.0	38.0
180-184	34.35191473624046	37.6	34.8	38.0	23.6	38.0
185-189	33.603680866086144	37.0	33.8	37.8	22.6	38.0
190-194	32.60189153750461	36.8	31.0	37.0	20.4	38.0
195-199	32.364483744074235	36.8	30.4	37.2	20.2	37.8
200-204	32.16533974380527	37.0	30.0	37.0	19.8	37.4
205-209	31.0418727095451	35.4	27.8	37.0	17.2	37.2
210-214	30.349182664828696	34.4	26.0	37.0	15.8	37.0
215-219	29.504156272064016	33.2	24.6	37.0	13.6	37.0
220-224	28.023601519685304	31.6	22.0	36.2	9.4	37.0
225-229	27.01910903405843	30.0	20.0	36.2	9.0	37.0
230-234	26.610077665333016	29.4	20.0	35.8	9.0	37.0
235-239	25.605661836398486	28.0	18.4	34.6	8.8	37.0
240-244	24.47750932992637	26.4	16.6	33.8	8.0	37.0
245-249	23.695363614968226	25.0	15.2	33.0	8.0	36.4
250-254	22.824977305584508	23.8	14.0	32.0	8.0	36.0
255-259	22.251945667888243	22.6	13.2	31.0	8.0	36.0
260-264	20.997168409373636	20.8	11.8	29.6	7.8	35.4
265-269	20.260730928285646	19.6	10.4	28.8	7.0	34.4
270-274	19.302871936253908	18.8	9.0	27.2	7.0	33.6
275-279	18.020478768113506	17.0	8.0	25.2	7.0	32.0
280-284	17.81885149446929	17.2	8.0	25.0	7.0	31.0
285-289	16.674077261876743	14.8	8.0	22.6	7.0	30.2
290-294	15.972431832700133	13.6	8.0	21.6	7.0	29.2
295-299	15.197667350300913	13.0	8.0	21.0	7.0	27.0
300-301	13.312397875130284	10.5	8.0	17.0	7.0	23.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.09055593734953504
1101	2	0.035115318098263515
1101	3	0.06119309874898704
1101	4	0.064164853545158
1101	5	0.06597507410452152
1101	6	0.11637427140279755
1101	7	0.07796135952349914
1101	8	0.1208981890857288
1101	9	0.06309358096360285
1101	10-14	0.01439995375449854
1101	15-19	0.07619199548408773
1101	20-24	0.053158693252271405
1101	25-29	0.13466789769651655
1101	30-34	0.16153376640072992
1101	35-39	0.06839059018672344
1101	40-44	0.10294400735457998
1101	45-49	0.027759890603689996
1101	50-54	0.038657135529824416
1101	55-59	0.14132220869593226
1101	60-64	0.2810843510577925
1101	65-69	0.3251781399399576
1101	70-74	0.1831702684635701
1101	75-79	0.22329346276585937
1101	80-84	0.2622289086649303
1101	85-89	0.26239203671985933
1101	90-94	0.44235768818149523
1101	95-99	0.3090354300135445
1101	100-104	0.3399400958986476
1101	105-109	0.40895881970708814
1101	110-114	0.3997408105307656
1101	115-119	0.23493778620287742
1101	120-124	0.3486468373070366
1101	125-129	0.3975825086296467
1101	130-134	0.3857434401754958
1101	135-139	0.32415697366104723
1101	140-144	0.3241002340846677
1101	145-149	0.6990604762957986
1101	150-154	0.9743330052069297
1101	155-159	0.536095475180808
1101	160-164	0.40350112249450376
1101	165-169	0.2645740703465691
1101	170-174	0.4188457613229488
1101	175-179	0.527025380295413
1101	180-184	0.387505769411689
1101	185-189	0.5796744431669651
1101	190-194	0.6884350347789265
1101	195-199	0.340851204258783
1101	200-204	0.34064172124956116
1101	205-209	0.17281850203960403
1101	210-214	-0.08820565182761797
1101	215-219	-0.10317582890453636
1101	220-224	0.5512714020125031
1101	225-229	1.167803861307192
1101	230-234	0.6543707981141047
1101	235-239	0.8867057785032735
1101	240-244	0.8514475054623922
1101	245-249	0.8129647474417432
1101	250-254	0.3929830079170813
1101	255-259	0.03022453674817882
1101	260-264	0.7637788184196808
1101	265-269	0.586864757319681
1101	270-274	0.47925131226591944
1101	275-279	0.7905021651829856
1101	280-284	0.6264579350587525
1101	285-289	0.5665215881118826
1101	290-294	0.26176160493454503
1101	295-299	0.4865146829482203
1101	300-301	0.49068706236922033
1102	1	-0.11924905060008939
1102	2	-0.11093143831783436
1102	3	0.019651021997127316
1102	4	-0.02522645488360098
1102	5	-0.02735841566457964
1102	6	0.1117163964262673
1102	7	0.0746208971887583
1102	8	-0.06954671423696368
1102	9	0.12569414681412638
1102	10-14	0.054650429766191166
1102	15-19	0.03097256079825428
1102	20-24	0.022272574021393154
1102	25-29	-0.06704607961582099
1102	30-34	-0.00986161829717247
1102	35-39	-0.029627592119041424
1102	40-44	-0.047547424434952745
1102	45-49	-0.03246396897956316
1102	50-54	-0.023626647891568098
1102	55-59	-0.12650912225957
1102	60-64	0.11916126382026704
1102	65-69	0.1422173472128705
1102	70-74	0.08163261660913435
1102	75-79	-0.14268578044259073
1102	80-84	0.05802324479622456
1102	85-89	0.0637798454570202
1102	90-94	0.16598831651693047
1102	95-99	0.07097024993362311
1102	100-104	0.015145585453218757
1102	105-109	-0.015041555738754653
1102	110-114	-0.015189866076276815
1102	115-119	0.07295905252806989
1102	120-124	0.0160008156920739
1102	125-129	0.01909555537265817
1102	130-134	0.13723583739719913
1102	135-139	0.14070240260792843
1102	140-144	0.016508518028992114
1102	145-149	0.3291856472577379
1102	150-154	0.6001462477181221
1102	155-159	0.45380143404266704
1102	160-164	0.5512062097856614
1102	165-169	0.3988157367897571
1102	170-174	0.3346064414162768
1102	175-179	0.41009344326304387
1102	180-184	0.43356895158338205
1102	185-189	0.7141464054078384
1102	190-194	0.8660142136252347
1102	195-199	0.5959012860523387
1102	200-204	0.21454052518637923
1102	205-209	-0.14069184953860514
1102	210-214	-0.3499845946794018
1102	215-219	-0.23737693378801694
1102	220-224	0.4651977609923641
1102	225-229	0.9577734233764339
1102	230-234	0.5457348733105434
1102	235-239	0.5799390376413491
1102	240-244	1.0163403022657356
1102	245-249	0.9146157034877582
1102	250-254	0.3307206785438055
1102	255-259	-0.07305223388122783
1102	260-264	0.5497016106640444
1102	265-269	0.40907003233376216
1102	270-274	0.6272654719376725
1102	275-279	0.9905785952293833
1102	280-284	0.32112224624972185
1102	285-289	0.713685295907748
1102	290-294	0.3436791945722426
1102	295-299	0.3662731237752759
1102	300-301	0.11271701758389518
1103	1	-0.0036248883341798432
1103	2	-0.0182054783054042
1103	3	0.01995692342779165
1103	4	-0.08017852210297605
1103	5	-7.713052983078228E-4
1103	6	0.015768603603767417
1103	7	0.004452700740834814
1103	8	0.04607251413489877
1103	9	0.05615091027831198
1103	10-14	0.06872551376573455
1103	15-19	-0.039899866792744376
1103	20-24	0.017481762905660503
1103	25-29	0.0421699410670584
1103	30-34	0.1399675867373773
1103	35-39	-0.4729340247656424
1103	40-44	-0.26979040555952594
1103	45-49	-0.057648208806291734
1103	50-54	0.051824104445053365
1103	55-59	-0.26732476073013345
1103	60-64	0.1772236578443298
1103	65-69	0.3656579488848095
1103	70-74	0.24375971700894183
1103	75-79	0.24344109628736277
1103	80-84	0.19272940673648264
1103	85-89	0.24175527137542474
1103	90-94	0.26393663891072805
1103	95-99	0.15804854589819683
1103	100-104	0.1676012667499691
1103	105-109	4.5712115532836606E-4
1103	110-114	0.3186739156877181
1103	115-119	0.1813918997631987
1103	120-124	0.2884168942659855
1103	125-129	0.3982261703496519
1103	130-134	0.3878174612732934
1103	135-139	0.43407338703490694
1103	140-144	0.37665285087606293
1103	145-149	0.7824647954702613
1103	150-154	0.9240060659205795
1103	155-159	0.11723420726938372
1103	160-164	-0.5187633151954643
1103	165-169	0.5070854365952044
1103	170-174	1.0758404741016747
1103	175-179	0.7400078073087784
1103	180-184	0.742437955051912
1103	185-189	0.7843761110681697
1103	190-194	0.4533005554552787
1103	195-199	0.6745728844202077
1103	200-204	0.6749751354663189
1103	205-209	0.9453450658566211
1103	210-214	0.9043941540521772
1103	215-219	0.5714231596270416
1103	220-224	1.4368528686417115
1103	225-229	2.0230521308277645
1103	230-234	0.9402977220751794
1103	235-239	1.1462553430099263
1103	240-244	1.2108613773941457
1103	245-249	1.6554882462029497
1103	250-254	1.7102341636985408
1103	255-259	0.7184927163604478
1103	260-264	1.7310472151400695
1103	265-269	0.6100745731609152
1103	270-274	0.7769831709668473
1103	275-279	1.644609339967797
1103	280-284	0.945335166802515
1103	285-289	1.0768511454667404
1103	290-294	1.5247376464149696
1103	295-299	1.2079467664199086
1103	300-301	-0.8447034768251793
2116	1	0.02223199960742761
2116	2	-0.025237783538429426
2116	3	0.008821407498224687
2116	4	0.03578790870781745
2116	5	-0.004481078653611803
2116	6	-0.16978202279804577
2116	7	-0.2650751124910471
2116	8	-0.03362797781667126
2116	9	0.03800012774073025
2116	10-14	0.02639227543564715
2116	15-19	0.013207049030256712
2116	20-24	-0.24729846981097126
2116	25-29	-0.34375956239643557
2116	30-34	-0.0773402485517849
2116	35-39	-0.30917053327256383
2116	40-44	-0.11609519248376898
2116	45-49	0.031490442230271753
2116	50-54	0.10478665664520292
2116	55-59	0.11011640954459523
2116	60-64	0.319480996844824
2116	65-69	0.17402639211210413
2116	70-74	-0.8480456337388915
2116	75-79	0.10774486708996278
2116	80-84	-0.05337525379214014
2116	85-89	0.0533548574917333
2116	90-94	0.2985786419095291
2116	95-99	0.25608786484885826
2116	100-104	0.10108123552281256
2116	105-109	0.1584284095232178
2116	110-114	0.3185577432444404
2116	115-119	0.2660602351824721
2116	120-124	-0.1290734092074146
2116	125-129	0.05444248034110899
2116	130-134	0.1641734260312191
2116	135-139	0.3000940316525629
2116	140-144	0.4880399188694753
2116	145-149	0.6343378050490713
2116	150-154	0.9190137487914924
2116	155-159	-0.31849888731666454
2116	160-164	-0.48306181951397775
2116	165-169	0.6888643310052913
2116	170-174	0.5907542276797884
2116	175-179	0.5567738143224901
2116	180-184	0.569517286889976
2116	185-189	0.7434499533710479
2116	190-194	0.7060143437302244
2116	195-199	0.8747494665339914
2116	200-204	0.7246662716637928
2116	205-209	0.7525189465738613
2116	210-214	0.5746403776732869
2116	215-219	0.8386154420592895
2116	220-224	1.1200428709961407
2116	225-229	0.9644243907070695
2116	230-234	1.0536888421634103
2116	235-239	0.90082479244548
2116	240-244	1.4972825226375797
2116	245-249	1.057521728650066
2116	250-254	1.0465645395396308
2116	255-259	0.8032468644911575
2116	260-264	0.09570445996342869
2116	265-269	0.475319495650659
2116	270-274	0.8466033025669901
2116	275-279	1.6703667842979648
2116	280-284	0.18132507993651004
2116	285-289	-1.3748579835187478
2116	290-294	-0.07731194527761609
2116	295-299	0.4444834645202551
2116	300-301	0.9514998528319403
1117	1	0.007895801836710348
1117	2	0.06030303469433562
1117	3	0.05713232349548747
1117	4	0.043398905656566455
1117	5	0.05275629602724763
1117	6	-0.5117366899628806
1117	7	-0.3596124435826482
1117	8	-0.1968915683266701
1117	9	0.015293234409888612
1117	10-14	0.10210371450408218
1117	15-19	0.07374911267058337
1117	20-24	0.03553483238717092
1117	25-29	0.14974843934714244
1117	30-34	0.1194419296660385
1117	35-39	0.0252793079379785
1117	40-44	-0.007727921794433712
1117	45-49	0.15533626077439777
1117	50-54	0.05372837157140964
1117	55-59	0.275947096386858
1117	60-64	0.25065821834055413
1117	65-69	0.33926426495600026
1117	70-74	-0.10515504279300814
1117	75-79	0.16743413790384665
1117	80-84	-0.06971500097440497
1117	85-89	0.07634788320939379
1117	90-94	0.16311721840868643
1117	95-99	0.22193910091586133
1117	100-104	0.15348424940911798
1117	105-109	0.07182494729020306
1117	110-114	0.1837159100045156
1117	115-119	0.2129155810944212
1117	120-124	0.3255557706917003
1117	125-129	0.30557891473926446
1117	130-134	0.12967875014854258
1117	135-139	0.3216794354759642
1117	140-144	0.20541553378945565
1117	145-149	0.755315449983371
1117	150-154	0.9476727562016691
1117	155-159	-0.3183927163222151
1117	160-164	-0.9083632081434132
1117	165-169	0.3098933490014275
1117	170-174	0.6316194745818393
1117	175-179	0.7423295774459788
1117	180-184	0.5554469836332316
1117	185-189	0.8494876892201049
1117	190-194	0.7623472411709145
1117	195-199	0.38677823544861667
1117	200-204	0.5484619625536382
1117	205-209	0.9894001346926693
1117	210-214	0.5158496684136793
1117	215-219	0.09788501052556953
1117	220-224	0.923244191128159
1117	225-229	0.5969843259458756
1117	230-234	0.8777009226167323
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2108	175-179	-0.5274590450536678
2108	180-184	-0.4179185055614525
2108	185-189	-0.560309445125192
2108	190-194	-0.3624600601011281
2108	195-199	-1.1789975247064888
2108	200-204	-0.5004565601682636
2108	205-209	-0.1825296898687334
2108	210-214	0.3505430055300529
2108	215-219	-0.6673809846510501
2108	220-224	0.23211637157927711
2108	225-229	0.2373616844364399
2108	230-234	-0.18387660611074352
2108	235-239	-0.29522225586837436
2108	240-244	0.0011386977287735078
2108	245-249	-0.13062189774264965
2108	250-254	-0.07798011417386519
2108	255-259	-0.2758934367570127
2108	260-264	0.46749346217191956
2108	265-269	-0.6765750923043754
2108	270-274	-0.560061254866195
2108	275-279	-0.21245164792120264
2108	280-284	-0.33743841295413546
2108	285-289	-0.030475368565525685
2108	290-294	-0.1430629564689596
2108	295-299	0.11845614374306379
2108	300-301	-0.3071557589698397
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	27.0
14	125.0
15	235.0
16	341.0
17	407.0
18	762.0
19	812.0
20	807.0
21	1016.0
22	1511.0
23	2245.0
24	3449.0
25	4916.0
26	6844.0
27	9457.0
28	12607.0
29	17370.0
30	24083.0
31	33499.0
32	46209.0
33	56246.0
34	50594.0
35	21881.0
36	1981.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.320411525400935	23.439464748007936	15.580472716269375	30.65965101032176
2	42.38375416064284	24.349258649093905	18.23218908650775	15.034798103755506
3	41.87170090441448	22.9385065393538	19.950576606260295	15.239215949971422
4	42.33130484483744	22.52126550785059	19.728675654775913	15.418753992536057
5	28.883098544195274	22.730390343946475	33.14729516188683	15.239215949971422
6	27.559089533671788	22.71626937430656	33.56722590189288	16.15741519012877
7	17.795783881921796	45.90290152304744	20.56920956191373	15.732105033117035
8	27.077631711663248	21.628618498470228	34.46760582321891	16.826143966647614
9	16.089500050432033	20.230978717681474	36.22734761120264	27.452173620683855
10-14	25.84386242141008	34.073832498402986	23.570588037521432	16.511717042665502
15-19	21.9619406246848	34.19607974985711	22.453753824429278	21.388225801028813
20-24	24.363043405170963	31.038765423797194	26.68278250344619	17.91540866758565
25-29	19.499781461184142	26.202736778401643	36.36418653128467	17.93329522912954
30-34	19.718118548902265	28.684530813973037	30.57210099855428	21.025249638570422
35-39	21.00305954342198	29.560367145210638	27.627878828631946	21.808694482735433
40-44	20.196348720707395	29.283865111118583	29.08697844871062	21.432807719463405
45-49	21.365699492317518	30.375281578858893	26.170325790942407	22.088693137881183
50-54	21.974246041085298	29.587264230239047	27.639915274182158	20.798574454493494
55-59	21.687590357395017	27.08670947786034	29.72517903372222	21.500521131022428
60-64	22.06959620751101	26.202669535689072	29.36825471539522	22.3594795414047
65-69	20.408566721581547	26.972329623777025	29.095921729482566	23.523181925158863
70-74	21.15630568537135	26.347375853141912	29.120061863295565	23.376256598191173
75-79	20.491006287193624	25.960259556870525	30.526981138419124	23.021753017516726
80-84	23.074605789597552	26.054399354469957	27.41949366237434	23.451501193558148
85-89	20.93353057862354	28.325656456981473	26.089029351444037	24.651783612950947
90-94	20.421477322395184	30.006925999394817	28.50761523719867	21.063981441011332
95-99	22.29257304239653	30.018222775106747	26.45449349426756	21.234710688229164
100-104	22.587297851595334	28.494099451971895	26.64082305080187	22.277779645630904
105-109	23.50954746296437	27.72660977481073	24.333271245853645	24.43057151637126
110-114	21.69606293917897	28.36849006488922	28.6978448710621	21.237602124869717
115-119	23.400127761153886	28.20327472010221	26.816864472312812	21.579733046431095
120-124	19.245536764953098	28.777056786470766	28.001748310526846	23.975658138049287
125-129	20.93207938954294	25.487375836164254	30.368732634546124	23.211812139746684
130-134	24.622062333994556	28.94321352923377	25.99865514574858	20.4360689910231
135-139	23.63272030393706	27.867061157247086	25.035067074605788	23.465151464210066
140-144	21.39113162164652	29.270637979113058	26.59625901641329	22.741971382827142
145-149	20.905019668493427	27.505026392764687	28.886258951686113	22.703694987055776
150-154	23.277544296136906	26.315166593820393	25.793430386981807	24.61385872306089
155-159	21.88777191271896	27.453854688498136	29.364354638066096	21.294018760716806
160-164	24.327673738358605	29.517600780015467	26.06159432471506	20.09313115691087
165-169	20.14154590996201	26.44783646572303	28.316511448071815	25.094106176243148
170-174	22.121440338903273	29.470261910365465	26.02817469656726	22.380123054164006
175-179	20.11007632047877	29.824294792051916	28.19399522576741	21.871633661701914
180-184	19.88333389368927	29.90733954207713	26.749285546178932	23.460041018054667
185-189	21.78610093131157	28.156541034865345	27.620818343811987	22.436539690011095
190-194	19.89779107689204	28.481928520996536	26.66563561174058	24.954644790370846
195-199	20.648031905343714	30.474552314529348	27.15154971021734	21.725866069909607
200-204	21.77762834952762	28.420804895269473	27.20586356453619	22.59570319066671
205-209	20.984904011027805	28.12540765894496	28.055407995158525	22.83428033486871
210-214	22.34757758127963	28.454964193255556	27.236324513330867	21.961133712133947
215-219	22.529148054431666	27.765274360538182	27.757406957877116	21.94817062715303
220-224	18.943953199072048	27.912181017382242	28.18491745957032	24.958948323975388
225-229	20.9498705577783	29.44840802877988	26.458797027872105	23.142924385569714
230-234	21.17936993578321	27.360723531587265	28.063275392529334	23.39663114010019
235-239	21.20559459368591	28.103419291934234	25.691759405574423	24.999226708805434
240-244	22.30635779847359	32.02037454190902	23.507581615842383	22.165686043775008
245-249	20.145311501865983	30.38375416064284	24.71357966580372	24.757354671687455
250-254	21.13936052180345	27.963756177924214	25.524123323134855	25.372759977137477
255-259	20.984635040177523	28.63504017752076	26.977776283495274	23.402548498806443
260-264	20.528796691658542	29.851595333355746	26.51185152809064	23.107756446895067
265-269	19.85281905175608	29.231637179596664	26.888899498301782	24.026644270345475
270-274	18.743179062756994	31.410437017407133	25.06984841465112	24.776535505184754
275-279	19.869885351175064	29.99804996133544	24.423561846484887	25.708502841004606
280-284	17.205325622835623	31.076555828262116	26.75836331237602	24.95975523652624
285-289	19.923007094106175	28.736778401640724	27.93968328682379	23.40053121742931
290-294	19.14662273476112	31.73116363514104	25.55808089298322	23.564132737114615
295-299	18.414282352150085	32.45899875600982	23.18004236290892	25.946676528931178
300-301	14.613522509498033	35.59711528763071	27.23329859126517	22.556063611606092
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.25
19	0.25
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.25
26	0.0
27	0.0
28	0.0
29	0.5
30	0.25
31	0.5
32	4.75
33	9.0
34	29.5
35	80.0
36	204.5
37	677.25
38	1725.0
39	2706.5
40	4934.75
41	7017.75
42	9528.75
43	12898.5
44	15796.25
45	12310.75
46	8688.25
47	6794.0
48	6001.0
49	4187.5
50	3537.75
51	1452.75
52	285.5
53	63.75
54	32.5
55	24.0
56	13.75
57	11.75
58	8.75
59	6.0
60	4.0
61	1.75
62	1.75
63	2.0
64	1.5
65	1.0
66	0.75
67	0.5
68	0.25
69	0.0
70	0.0
71	0.25
72	0.5
73	0.0
74	0.25
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	6.724271257102511E-5
110-114	0.0
115-119	0.0
120-124	0.0
125-129	1.3448542514205022E-4
130-134	0.0
135-139	0.0
140-144	6.724271257102511E-5
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	6.724271257102511E-5
200-204	0.0
205-209	0.0
210-214	0.0
215-219	6.724271257102511E-5
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	6.724271257102511E-5
270-274	6.724271257102511E-5
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	297430.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.716219861206437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	35.04976570579694	4.106507610928904
2	8.095478261799292	1.8969680639371564
3	6.012720494208376	2.113390658223815
4	5.460187197515182	2.5589101475772997
5	5.440712472320721	3.18722917636588
6	5.206753081140401	3.660207831699299
7	4.557302066022106	3.737604708561173
8	4.115582987127936	3.857526010738531
9	3.419006912115831	3.605205302040023
>10	22.07335737532231	44.05829746471057
>50	0.4426652250134465	3.2696312356366177
>100	0.06041176022014846	1.1989581613034312
>500	0.014360100303737687	1.27564187248254
>1k	0.0430803009112128	13.213375111749967
>5k	0.005744040121495041	4.6415731967882
>10k+	0.0028720200607475206	3.6189734472565673
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	10755	3.615976868506875	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	7195	2.4190565847426284	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	6599	2.2186733012809734	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	4632	1.5573412231449417	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	4525	1.5213663719194432	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	3992	1.3421645429176614	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3368	1.1323672796960629	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3224	1.0839525266449248	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	2876	0.9669502067713411	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2765	0.9296305012944222	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	2668	0.897017785697475	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2631	0.8845778838718353	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1675	0.5631577177823354	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1629	0.5476918938909996	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1585	0.5328984971253741	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1397	0.4696903473086105	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1216	0.4088356924318327	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	1085	0.36479171569781127	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	840	0.2824193927983055	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	818	0.2750226944154927	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT	788	0.26493628752983894	TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	706	0.23736677537571868	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	639	0.21484046666442524	No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC	392	0.13179571663920922	No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT	307	0.10321756379652355	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	6.724271257102512E-4	0.0	0.0
2	6.724271257102512E-4	0.0	0.0
3	6.724271257102512E-4	0.0	0.0
4	6.724271257102512E-4	0.0	0.0
5	6.724271257102512E-4	0.0	0.0
6	6.724271257102512E-4	0.0	0.0
7	6.724271257102512E-4	0.0	0.0
8	6.724271257102512E-4	0.0	0.0
9	6.724271257102512E-4	0.0	0.0
10-14	8.069125508523014E-4	0.0	0.0
15-19	0.0014120969639915275	0.0	0.0
20-24	0.001748310526846653	0.0	0.0
25-29	0.0026224657902699797	0.0	0.0
30-34	0.005850115993679185	0.0	0.0
35-39	0.0073294556702417375	0.0	0.0
40-44	0.009481222472514542	0.0	0.0
45-49	0.012843358101065797	0.0	0.0
50-54	0.017752076118750633	0.0	0.0
55-59	0.026897085028410046	0.0	0.0
60-64	0.037454190902060994	0.0	0.0
65-69	0.05285277208082574	0.0	0.0
70-74	0.07860673099552837	0.0	0.0
75-79	0.11949030023871163	0.0	0.0
80-84	0.17920182900178194	0.0	0.0
85-89	0.2514877450156339	0.0	0.0
90-94	0.34206367884880473	0.0	0.0
95-99	0.4425915341424873	0.0	0.0
100-104	0.564838785596611	0.0	0.0
105-109	0.7154624617557073	0.0	0.0
110-114	0.9025316881282992	0.0	0.0
115-119	1.12759304710352	0.0	0.0
120-124	1.3755841710654608	0.0	0.0
125-129	1.6477154288403995	0.0	0.0
130-134	1.9668493427024845	0.0	0.0
135-139	2.2799986551457483	0.0	0.0
140-144	2.6607268937228925	0.0	0.0
145-149	3.0952493023568564	0.0	0.0
150-154	3.56971388225801	0.0	0.0
155-159	4.09380358403658	0.0	0.0
160-164	4.658776855058333	0.0	0.0
165-169	5.267726860101536	0.0	0.0
170-174	5.919913929327909	0.0	0.0
175-179	6.616951887839155	0.0	0.0
180-184	7.341626601217092	0.0	0.0
185-189	8.193591769491983	0.0	0.0
190-194	9.089197458225465	0.0	0.0
195-199	9.992872272467471	0.0	0.0
200-204	10.922166560199038	0.0	0.0
205-209	11.943717849578054	0.0	0.0
210-214	12.992771408398616	0.0	0.0
215-219	14.050835490703696	0.0	0.0
220-224	15.18663214874088	0.0	0.0
225-229	16.430622331304843	0.0	0.0
230-234	17.70009750193323	0.0	0.0
235-239	18.966277779645633	0.0	0.0
240-244	20.165148102074436	0.0	0.0
245-249	21.35453720203073	0.0	0.0
250-254	22.6490939044481	0.0	0.0
255-259	23.933295229129545	0.0	1.3448542514205025E-4
260-264	25.198332380728242	0.0	3.362135628551256E-4
265-269	26.36660726893723	0.0	3.362135628551256E-4
270-274	27.46750495915005	0.0	3.362135628551256E-4
275-279	28.495444306223312	0.0	3.362135628551256E-4
280-284	29.508321285680665	0.0	3.362135628551256E-4
285-289	30.420804895269477	0.0	3.362135628551256E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	4015	0.0	293.16312	1
GGGTTAT	4055	0.0	290.99875	2
GGGGGGA	3435	0.0	288.55896	1
GTTATTA	4115	0.0	286.3973	4
GGTTATT	4190	0.0	281.62292	3
TTATTAG	4265	0.0	276.32474	5
TTAGTAG	4310	0.0	274.46634	8
GGGGAGC	3605	0.0	273.31485	3
TAGTAGA	4335	0.0	272.88348	9
GGGAGCG	2455	0.0	270.9674	4
GGAGCGA	2485	0.0	267.69617	5
TATTAGT	4510	0.0	262.62198	6
CGAAAGC	2540	0.0	260.7382	9
ATTAGTA	4555	0.0	260.02747	7
GCGAAAG	2690	0.0	246.19888	8
GAGCGAA	2725	0.0	243.57797	6
AGCGAAA	2750	0.0	240.29091	7
GGGGGAG	4160	0.0	238.26923	2
AAACGTT	180	0.0	221.25002	295
AACCGTT	55	0.0	214.54547	295
>>END_MODULE