##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_30291_flu.3510000003ba0f.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	233962
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.822248912216516	34.0	34.0	34.0	34.0	34.0
2	33.88898197143126	34.0	34.0	34.0	34.0	34.0
3	33.89400415452082	34.0	34.0	34.0	34.0	34.0
4	33.898979321428264	34.0	34.0	34.0	34.0	34.0
5	33.8863362426377	34.0	34.0	34.0	34.0	34.0
6	37.64841726434207	38.0	38.0	38.0	38.0	38.0
7	37.66746309229704	38.0	38.0	38.0	38.0	38.0
8	37.67100212855079	38.0	38.0	38.0	38.0	38.0
9	37.59437002590164	38.0	38.0	38.0	38.0	38.0
10-14	37.66305297441465	38.0	38.0	38.0	38.0	38.0
15-19	37.63334729571469	38.0	38.0	38.0	37.4	38.0
20-24	37.62604440037271	38.0	38.0	38.0	37.2	38.0
25-29	37.55104076730409	38.0	38.0	38.0	37.0	38.0
30-34	37.497132867730656	38.0	38.0	38.0	37.0	38.0
35-39	37.41837905300861	38.0	38.0	38.0	37.0	38.0
40-44	37.42957745274873	38.0	38.0	38.0	37.0	38.0
45-49	37.4018703892085	38.0	38.0	38.0	37.0	38.0
50-54	37.36667749463588	38.0	38.0	38.0	37.0	38.0
55-59	37.32236517041229	38.0	38.0	38.0	37.0	38.0
60-64	37.142813790273635	38.0	38.0	38.0	36.6	38.0
65-69	37.07688000615485	38.0	38.0	38.0	36.4	38.0
70-74	37.16764175378908	38.0	38.0	38.0	37.0	38.0
75-79	37.11050512476385	38.0	38.0	38.0	36.8	38.0
80-84	37.020484523127685	38.0	38.0	38.0	36.2	38.0
85-89	37.06098340756192	38.0	38.0	38.0	36.0	38.0
90-94	36.910331592309866	38.0	38.0	38.0	35.8	38.0
95-99	36.912814046725536	38.0	38.0	38.0	36.0	38.0
100-104	36.89191150699686	38.0	38.0	38.0	36.0	38.0
105-109	36.735559620793126	38.0	38.0	38.0	35.0	38.0
110-114	36.63885160838085	38.0	37.8	38.0	34.8	38.0
115-119	36.738213897983435	38.0	38.0	38.0	35.0	38.0
120-124	36.65256494644429	38.0	37.8	38.0	34.8	38.0
125-129	36.54279754831981	38.0	37.0	38.0	34.0	38.0
130-134	36.42174968584642	38.0	37.0	38.0	34.0	38.0
135-139	36.18664911395867	38.0	37.0	38.0	33.6	38.0
140-144	36.132540327061655	38.0	37.0	38.0	33.0	38.0
145-149	35.717394277703214	38.0	36.8	38.0	30.4	38.0
150-154	35.34767697318368	38.0	36.2	38.0	28.6	38.0
155-159	35.42509467349398	38.0	36.0	38.0	29.2	38.0
160-164	35.37065762816184	38.0	36.0	38.0	28.6	38.0
165-169	35.17456680999478	38.0	35.6	38.0	28.2	38.0
170-174	35.03506637830075	38.0	35.0	38.0	28.0	38.0
175-179	34.67205358135082	38.0	35.0	38.0	25.2	38.0
180-184	34.39610022140347	37.6	35.0	38.0	23.8	38.0
185-189	33.61660526068336	37.0	33.8	37.8	22.8	38.0
190-194	32.63526384626563	36.8	30.8	37.0	21.2	38.0
195-199	32.36482249254152	36.8	30.2	37.2	20.4	37.8
200-204	32.18885630999906	37.0	30.0	37.0	20.0	37.4
205-209	31.024726237594138	35.4	27.8	37.0	17.8	37.2
210-214	30.32155478240056	34.4	26.2	37.0	16.0	37.0
215-219	29.499217821697545	33.2	24.6	37.0	14.0	37.0
220-224	27.95421478701669	31.4	22.0	36.2	9.4	37.0
225-229	26.96930954599465	29.8	20.0	36.0	9.0	37.0
230-234	26.517648165086637	29.4	19.8	35.6	9.0	37.0
235-239	25.470567869995982	27.6	18.4	34.6	8.8	37.0
240-244	24.315697420948702	25.8	16.6	33.2	8.0	37.0
245-249	23.550245766406512	25.0	15.2	32.6	8.0	36.2
250-254	22.64610492302169	23.6	13.8	31.6	8.0	36.0
255-259	22.04878399056257	22.2	13.2	30.8	8.0	36.0
260-264	20.77144579034202	20.2	11.6	29.2	7.8	35.2
265-269	20.08799463160684	19.6	10.2	28.4	7.2	34.0
270-274	19.069323223429443	18.6	8.8	26.8	7.0	33.0
275-279	17.814095451398092	17.0	8.0	25.0	7.0	31.4
280-284	17.59487865550816	16.8	8.0	24.6	7.0	30.8
285-289	16.472505791538797	14.6	8.0	22.6	7.0	29.4
290-294	15.802606406168522	13.4	8.0	21.4	7.0	28.6
295-299	15.013135466443268	13.0	8.0	20.2	7.0	27.0
300-301	13.219110368350416	10.5	8.0	16.5	7.0	23.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.10897518309216281
1101	2	0.06552052001441666
1101	3	0.017174115253538957
1101	4	0.047615708250724254
1101	5	0.057054606332030744
1101	6	0.1712354987848883
1101	7	0.11320481979601738
1101	8	0.056454723877607194
1101	9	0.14259515858465477
1101	10-14	0.021858376071570262
1101	15-19	0.057708169480690685
1101	20-24	0.14808082554877444
1101	25-29	0.20747592694067407
1101	30-34	0.18568123824071847
1101	35-39	0.18084583247606645
1101	40-44	0.20152684642717134
1101	45-49	0.1273145784106049
1101	50-54	0.1708956993669304
1101	55-59	0.1794432428048367
1101	60-64	0.2924337154908585
1101	65-69	0.390547021244096
1101	70-74	0.32204150251000385
1101	75-79	0.23145887489744155
1101	80-84	0.3650571620014773
1101	85-89	0.2430146892327656
1101	90-94	0.3818457972883067
1101	95-99	0.2921834422735756
1101	100-104	0.43079902602423203
1101	105-109	0.39950862554922395
1101	110-114	0.41453137958679065
1101	115-119	0.44612169109988287
1101	120-124	0.2851395204034688
1101	125-129	0.3970814249791843
1101	130-134	0.40737232312400806
1101	135-139	0.33849051507168326
1101	140-144	0.42977202795639613
1101	145-149	0.7913407083473842
1101	150-154	1.02074255368332
1101	155-159	0.6642877788149733
1101	160-164	0.6486376359753834
1101	165-169	0.6679323152465386
1101	170-174	0.5990133525889405
1101	175-179	0.8734440639184413
1101	180-184	0.5444256020007572
1101	185-189	0.7174292055132909
1101	190-194	0.686959474951685
1101	195-199	0.534383525672169
1101	200-204	0.28024872435798187
1101	205-209	0.5350472596027096
1101	210-214	0.6831945160794
1101	215-219	0.48082805231488024
1101	220-224	1.5238001620071842
1101	225-229	1.2876470627984737
1101	230-234	1.2695821467558304
1101	235-239	1.2916468291901921
1101	240-244	1.3933123352318866
1101	245-249	1.4109718816588028
1101	250-254	1.1385929324951967
1101	255-259	0.8092643761291463
1101	260-264	1.1703353323412529
1101	265-269	0.5197529124238294
1101	270-274	0.5764573126473422
1101	275-279	0.7943932620844407
1101	280-284	0.5992411157105906
1101	285-289	0.7990342955000465
1101	290-294	0.8282552617586258
1101	295-299	0.6669734441713224
1101	300-301	0.6269672418258843
1102	1	-0.059946323455669415
1102	2	-0.10010383020390634
1102	3	-0.05195465747882366
1102	4	-0.06540764923449416
1102	5	-0.05073317697278412
1102	6	-0.04789354052456929
1102	7	0.11112818421224091
1102	8	0.0022516027929597726
1102	9	0.004606855987660197
1102	10-14	0.03770958339924135
1102	15-19	0.028101583960086884
1102	20-24	0.07424696705855638
1102	25-29	0.06995786634236367
1102	30-34	0.1076925091203691
1102	35-39	0.11774223528472305
1102	40-44	0.03577400604820724
1102	45-49	0.17391172177043757
1102	50-54	0.17181005880882338
1102	55-59	0.06004815955281373
1102	60-64	0.22691599611123792
1102	65-69	0.3014974752248847
1102	70-74	0.11461626405679226
1102	75-79	-0.07243954856561174
1102	80-84	0.10050765063729727
1102	85-89	0.14879781276992787
1102	90-94	0.24368368642422666
1102	95-99	0.09198297629680496
1102	100-104	0.19031129656799806
1102	105-109	0.1669041139984344
1102	110-114	0.3049020816975485
1102	115-119	0.12840604355746166
1102	120-124	0.2579006726563051
1102	125-129	0.08851257078087116
1102	130-134	0.149245187175012
1102	135-139	0.22629727866578975
1102	140-144	0.40295330571373
1102	145-149	0.24016110469420937
1102	150-154	0.9211029659181449
1102	155-159	0.6692002615541028
1102	160-164	0.46980279114991674
1102	165-169	0.5473269719752096
1102	170-174	0.5099883316626332
1102	175-179	0.5585717276900795
1102	180-184	0.7719864748807979
1102	185-189	0.9054652254106017
1102	190-194	0.8994774022614394
1102	195-199	0.9248736543222762
1102	200-204	0.374560502267272
1102	205-209	0.20565286945113215
1102	210-214	-0.05756298939876103
1102	215-219	-0.19083533545349596
1102	220-224	0.6518515367452729
1102	225-229	1.3424483828882536
1102	230-234	1.209684405730144
1102	235-239	1.2222066944088112
1102	240-244	1.1835609450323297
1102	245-249	1.0084346008518246
1102	250-254	0.4567686921066354
1102	255-259	0.25654982155282013
1102	260-264	0.7524879957130253
1102	265-269	0.8835831987809435
1102	270-274	0.9611312622002934
1102	275-279	1.0783005359071076
1102	280-284	0.3998275853234645
1102	285-289	1.2930272472016142
1102	290-294	0.45964631436495473
1102	295-299	0.8585704009271868
1102	300-301	0.5456465655731044
1103	1	0.02907158588163128
1103	2	0.012451306108999916
1103	3	-0.024479502034282064
1103	4	-0.005432626648534722
1103	5	-0.008949151889076745
1103	6	-0.15236821767820885
1103	7	0.04451288953190158
1103	8	-0.010436392105269476
1103	9	0.19340422007332592
1103	10-14	0.06833295588156574
1103	15-19	-0.023561274093800932
1103	20-24	0.03814450651756118
1103	25-29	0.10277606265421468
1103	30-34	0.09537014104894581
1103	35-39	-0.6246662251499941
1103	40-44	-0.2718127506080066
1103	45-49	-0.11924978112588036
1103	50-54	0.05263909638123465
1103	55-59	-0.33971428929663716
1103	60-64	0.1700493329874604
1103	65-69	0.25220481433314745
1103	70-74	0.13139842912029565
1103	75-79	0.30707950335552425
1103	80-84	0.2747878228220131
1103	85-89	0.2191113590312881
1103	90-94	0.2837708416561924
1103	95-99	0.2873217656893772
1103	100-104	0.1132846717074969
1103	105-109	0.036456114848739674
1103	110-114	0.30433929273012694
1103	115-119	0.14162122132224653
1103	120-124	0.4188445000464398
1103	125-129	0.3350290186737297
1103	130-134	0.40921489541756983
1103	135-139	0.47095423779848744
1103	140-144	0.4878010497749514
1103	145-149	0.8098937932205388
1103	150-154	0.7840508322092603
1103	155-159	0.131683122796602
1103	160-164	-0.5209656629075923
1103	165-169	0.38790517253853807
1103	170-174	0.7913705923843253
1103	175-179	0.6712820428306401
1103	180-184	0.7064404260951349
1103	185-189	0.8292362834963711
1103	190-194	0.5516520315859808
1103	195-199	0.42331974030836506
1103	200-204	0.43645187708791866
1103	205-209	0.8386374047118004
1103	210-214	0.38540560283167125
1103	215-219	0.4421476055041431
1103	220-224	1.0269006944218404
1103	225-229	1.749915566817684
1103	230-234	0.8491885774897234
1103	235-239	1.3419547945310448
1103	240-244	0.7548135357747441
1103	245-249	1.104121479737934
1103	250-254	0.9579968368693486
1103	255-259	0.2223722161959607
1103	260-264	1.5650665151369516
1103	265-269	0.48704177347404
1103	270-274	0.7323232694278019
1103	275-279	1.7249997972133713
1103	280-284	0.6901044626152775
1103	285-289	0.5551768991121193
1103	290-294	0.9044563265879457
1103	295-299	1.3472375635992382
1103	300-301	-0.8823108665361588
2116	1	-0.1313557821068656
2116	2	-0.011731969009140641
2116	3	0.06474383883278279
2116	4	0.06600768565884607
2116	5	0.0274182202087232
2116	6	-0.0344516366221157
2116	7	-0.049127956985458354
2116	8	0.10046354625511356
2116	9	0.07015581243001634
2116	10-14	0.08471060124832519
2116	15-19	0.1737348015174618
2116	20-24	-0.08185960279002558
2116	25-29	-0.13992786173452743
2116	30-34	0.10006913426857977
2116	35-39	-0.058465280009244225
2116	40-44	-0.08088199244075867
2116	45-49	0.13949688006171357
2116	50-54	0.18236788208083965
2116	55-59	0.16003504294088344
2116	60-64	0.3599630375282459
2116	65-69	0.212743133626347
2116	70-74	-0.6919609298277152
2116	75-79	0.17353522556633294
2116	80-84	-0.02417046770603548
2116	85-89	0.14094411021270759
2116	90-94	0.2881636914610084
2116	95-99	0.1528980960777062
2116	100-104	0.22045746809130407
2116	105-109	0.23762521285571836
2116	110-114	0.37154496357458555
2116	115-119	0.3532053593949769
2116	120-124	-0.016316790276142967
2116	125-129	0.11338324098726815
2116	130-134	0.2311175048278713
2116	135-139	0.4239006113345454
2116	140-144	0.4576222948951525
2116	145-149	0.6427970190269932
2116	150-154	1.3287929731585066
2116	155-159	-0.1851938034413294
2116	160-164	-0.36469075023038044
2116	165-169	0.7951868655732639
2116	170-174	1.0243436764196616
2116	175-179	0.5414340872111723
2116	180-184	0.9136070585175631
2116	185-189	0.8658399407801269
2116	190-194	0.6212698659808851
2116	195-199	0.9221259864558817
2116	200-204	0.8150095101267425
2116	205-209	0.8311132625297617
2116	210-214	1.0896505175635376
2116	215-219	1.2481697957650866
2116	220-224	1.5193601150094729
2116	225-229	1.4743227991990224
2116	230-234	1.5367476487862142
2116	235-239	1.2434855116052184
2116	240-244	2.0032704907778225
2116	245-249	1.388302700202285
2116	250-254	1.563994371284803
2116	255-259	1.5000833318729683
2116	260-264	0.8446453111098293
2116	265-269	0.9789813666525937
2116	270-274	1.5331204750986487
2116	275-279	1.9104446297590911
2116	280-284	0.5665601848260842
2116	285-289	-0.7875229159690438
2116	290-294	0.8435789894192549
2116	295-299	0.718277218417402
2116	300-301	1.9338746687011525
1117	1	0.03252635335898901
1117	2	0.09040490334111695
1117	3	0.03199755098292201
1117	4	-0.02355107581918503
1117	5	0.009138769144804826
1117	6	-0.3067998855320795
1117	7	-0.19706643274346192
1117	8	-0.03625810778655847
1117	9	0.05428750083042644
1117	10-14	0.07598127174602354
1117	15-19	0.08783996738376487
1117	20-24	0.05110268297018905
1117	25-29	0.169760900346283
1117	30-34	0.09744300153535335
1117	35-39	-0.07300244169623937
1117	40-44	-0.06056211460516181
1117	45-49	0.11160602430813071
1117	50-54	0.11639609241257176
1117	55-59	0.20937800743685386
1117	60-64	0.19113002902898302
1117	65-69	0.3839581469880713
1117	70-74	-0.09529232085426287
1117	75-79	0.20950038460107123
1117	80-84	0.1241487671590491
1117	85-89	0.21468579680476552
1117	90-94	0.23539188571847092
1117	95-99	0.21724035732466973
1117	100-104	0.19093909319930447
1117	105-109	0.2945552295327687
1117	110-114	0.41618319219079325
1117	115-119	0.29734829977956423
1117	120-124	0.38478731067023375
1117	125-129	0.3709301075106026
1117	130-134	0.1760460748212438
1117	135-139	0.3839480304106786
1117	140-144	0.21277510085869977
1117	145-149	0.6349105836957634
1117	150-154	0.9822710418357943
1117	155-159	-0.24820290549280344
1117	160-164	-0.7962083615948785
1117	165-169	0.3080985174047157
1117	170-174	0.5423759856884871
1117	175-179	0.6682017538309566
1117	180-184	0.6027750144740835
1117	185-189	0.6631123392611116
1117	190-194	1.1019043975165843
1117	195-199	0.8087110092612377
1117	200-204	0.9423427898791985
1117	205-209	1.5413676377432495
1117	210-214	0.5982844061287089
1117	215-219	0.30597786906372093
1117	220-224	0.9808642641786314
1117	225-229	0.5889905642736721
1117	230-234	1.413821731046255
1117	235-239	1.1360119839072524
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2108	170-174	-0.9760300921919551
2108	175-179	-1.0826345633305365
2108	180-184	-0.8235834303512917
2108	185-189	-1.3355598994721873
2108	190-194	-0.7911453960667494
2108	195-199	-1.896354567336079
2108	200-204	-0.8446838972093573
2108	205-209	-0.7979884639740185
2108	210-214	-0.15387662930192647
2108	215-219	-1.2091458287869017
2108	220-224	-0.6317550466838533
2108	225-229	-0.4258369484815603
2108	230-234	-0.966572235703925
2108	235-239	-1.0611154264003986
2108	240-244	-0.4091611556473076
2108	245-249	-0.12744371059501702
2108	250-254	-0.6937851359550464
2108	255-259	-0.7980447529981056
2108	260-264	-0.26203337076699995
2108	265-269	-0.9383850494696375
2108	270-274	-1.0378935130636435
2108	275-279	-0.3732078575942239
2108	280-284	-0.8491985142543435
2108	285-289	-0.11958816675241835
2108	290-294	-1.063241007713481
2108	295-299	-0.19423634989521155
2108	300-301	-0.30953573947465074
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	18.0
15	73.0
16	138.0
17	217.0
18	413.0
19	477.0
20	570.0
21	806.0
22	1177.0
23	1762.0
24	2687.0
25	4020.0
26	5577.0
27	7539.0
28	10417.0
29	14178.0
30	19863.0
31	27368.0
32	37281.0
33	44840.0
34	38142.0
35	15145.0
36	1252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.610629076516698	22.275839666270592	14.999016934373957	34.11451432283875
2	44.13366273155469	23.641446046793924	17.427616450534703	14.797274771116678
3	43.4314119386909	22.857130645147503	19.005223070413145	14.706234345748456
4	43.74513809934947	22.53998512578966	18.92358588146793	14.791290893392944
5	27.16894196493448	22.601533582376625	35.685282225318645	14.544242227370258
6	25.836674331729082	22.483138287414196	36.137492413297885	15.542694967558834
7	17.23698720305007	47.471811661722846	20.20926475239569	15.081936382831401
8	25.516109453671966	21.120951265590136	37.176977457877776	16.185961822860122
9	15.696993528863661	19.59933664441234	38.72851146767424	25.97515835904976
10-14	26.381634624426187	34.039117463519716	23.510142672741726	16.069105239312368
15-19	20.91177199716193	35.981826108513346	21.71703097084142	21.389370923483302
20-24	25.221189765859414	30.76311537771091	26.235969943837034	17.77972491259264
25-29	19.26928304596473	25.46216907019089	37.88059599422128	17.387951889623103
30-34	19.59882374060745	28.683375932843795	30.764568605158104	20.95323172139065
35-39	20.857404193843447	30.033595199220386	27.724331301664375	21.384669305271796
40-44	20.593087766389413	29.12763611184722	29.108829639001204	21.17044648276216
45-49	20.78021217120729	31.056410870141306	26.36496525076722	21.798411707884185
50-54	21.975192552636752	29.859635325394724	27.68193125379335	20.483240868175173
55-59	21.52700011112916	27.29024371479129	29.866986946598168	21.315769227481386
60-64	22.20505894119558	26.20348603619391	28.639693625460545	22.951761397149966
65-69	20.582829690291586	27.363247023020833	29.163881314059548	22.890041972628033
70-74	21.16437712107094	26.3064942170096	29.38588317761004	23.143245484309418
75-79	20.84680418187569	26.159205341038287	30.183961498021045	22.810028979064974
80-84	23.41611030851164	25.682546738359218	27.49292620169087	23.408416751438267
85-89	21.277130474179568	28.572503227019773	26.212547336746994	23.937818962053665
90-94	20.790897667142527	30.113351740881	28.649951701558372	20.4457988904181
95-99	21.981176430360485	30.754823432865162	26.490028295193234	20.773971841581112
100-104	22.532718988553697	28.836734170506322	26.361973311905352	22.26857352903463
105-109	23.855412417401116	27.57199887161163	24.094853010317916	24.47773570066934
110-114	21.473829083355415	28.063189748762618	29.38733640505723	21.075644762824734
115-119	23.38379736880348	27.830331421341924	27.083457997452577	21.702413212402014
120-124	18.557799984612885	29.39998803224455	27.89982988690471	24.14238209623785
125-129	20.920491361845087	25.926261529650112	29.829972388678506	23.323274719826298
130-134	24.459527615595693	29.343483129739017	25.66946769133449	20.527521563330797
135-139	24.017831955616725	27.550969815611083	24.935245894632462	23.495952334139734
140-144	21.398603191971347	29.13926193142476	26.630563937733477	22.831570938870417
145-149	20.81449124216753	27.343158290662583	28.678161410827403	23.164189056342483
150-154	23.060155067917012	26.2616151340816	25.618946666552688	25.059283131448694
155-159	21.938348962652054	27.756815209307494	29.36878638411366	20.93604944392679
160-164	24.23410639334593	29.052410220463155	26.19399731580342	20.5194860703875
165-169	19.710807738008736	25.829408194493126	28.19970764483121	26.260076422666927
170-174	22.38611398432224	28.88887939067028	26.69100110274318	22.034005522264298
175-179	20.468452141800807	29.446491310554705	28.339388447696635	21.745668099947853
180-184	19.870406305297443	29.833306263410297	26.545678358023956	23.750609073268308
185-189	22.057513613321824	28.063104264795136	27.571485967806737	22.307896154076303
190-194	20.622066831365775	28.378796556705787	26.40608303912601	24.59305357280242
195-199	20.874159051469896	30.192509894769238	27.43248903668117	21.500842017079698
200-204	21.418948376232038	28.442396628512324	27.4852326446175	22.653422350638138
205-209	21.382019302279858	27.761516827519	27.55815046887956	23.298313401321582
210-214	22.110513673160597	28.427265966268028	27.11636932493311	22.34585103563827
215-219	22.444841469982304	27.89871859532745	27.21971944161872	22.436720493071522
220-224	19.591386635436525	27.519597199545228	28.052589736794864	24.836426428223387
225-229	20.974346261358683	29.184739402125132	26.89599165676477	22.94492267975141
230-234	21.1848932732666	26.96326753917303	28.350244911566836	23.501594275993536
235-239	21.378770911515545	27.878202443131787	25.979859977261267	24.7631666680914
240-244	22.39064463459878	31.95510382027851	23.35533120763201	22.298920337490706
245-249	20.13019208247493	30.15566630478454	24.750344072969117	24.963797539771416
250-254	21.41168223899608	27.741428095160753	25.372838324172303	25.474051341670865
255-259	21.401167710995804	28.465648267667397	26.838460946649455	23.294723074687344
260-264	21.064788298954532	29.51060428616613	26.42847983860627	22.99612757627307
265-269	20.087193646831537	28.828955129465466	26.7088672519469	24.374983971756098
270-274	19.308605671006404	30.989647891537942	24.88378454620836	24.817961891247297
275-279	20.553850625315224	29.62617861020166	24.290183876014055	25.52978688846907
280-284	17.85375402843197	30.65600396645609	26.90471102144793	24.585530983664015
285-289	20.17908891187458	28.37324009881947	27.978304169053093	23.469366820252862
290-294	19.583522110428188	31.224386866243236	25.613903112471252	23.578187910857316
295-299	18.89024713414999	32.26942836870945	23.18744069549756	25.652883801643
300-301	14.898145852745317	35.191826023029385	27.712619998119354	22.197408126105948
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.25
25	0.25
26	0.0
27	0.0
28	0.0
29	0.25
30	0.25
31	0.75
32	1.25
33	3.25
34	12.0
35	44.0
36	118.5
37	495.0
38	1239.75
39	1999.75
40	3633.5
41	5174.75
42	7319.75
43	10201.25
44	12682.25
45	10252.0
46	7293.25
47	5521.75
48	4678.0
49	3202.5
50	2528.0
51	1181.75
52	239.25
53	46.0
54	16.75
55	9.75
56	6.0
57	4.25
58	3.5
59	1.5
60	3.0
61	1.5
62	0.5
63	0.5
64	0.5
65	0.75
66	0.5
67	0.0
68	0.75
69	0.5
70	0.0
71	0.25
72	0.0
73	0.0
74	0.0
75	0.25
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	233962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.586641881404752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	35.5032951315046	4.823705565615738
2	8.050372941503735	2.187550683359244
3	7.8748657954741175	3.209789442816912
4	7.371430149443308	4.006119263771045
5	6.5508598739720245	4.450209356146107
6	5.77465356272572	4.707488996755945
7	4.604006562167505	4.378709186586512
8	4.274992083367107	4.646622918603943
9	2.907662733101571	3.5554835039901853
>10	16.822964524179728	37.440716755754075
>50	0.15443837220066725	1.36947358897199
>100	0.04734317234036581	1.3640033349054996
>500	0.01577877450486689	1.4655054980773512
>1k	0.03786905881168053	13.242715861210375
>5k	0.009467264702920133	9.151906043435067
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	9021	3.8557543532710437	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	6951	2.970995289833392	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	5373	2.2965267864012104	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4503	1.9246715278549507	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	4168	1.7814858823227704	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	4141	1.769945546712714	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3321	1.419461280036929	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	2691	1.1501867824689478	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	2505	1.070686692710782	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	2416	1.032646327181337	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1653	0.7065249912378934	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1612	0.6890007779041041	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1464	0.625742641967499	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1355	0.5791538796898641	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1057	0.451782768141835	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	830	0.3547584650498799	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	799	0.34150845009018554	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	676	0.28893581008881786	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	561	0.23978252878672607	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	552	0.23593575025004063	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	448	0.19148408715945323	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	414	0.17695181268753046	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT	300	0.12822595122284816	TruSeq Adapter, Index 7 (97% over 36bp)
GGGAGCAAAAGCTATACTAAGAAAAGCAACCAGAAGATTGGTTCAACTCA	236	0.10087108162864056	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	4.2741983740949386E-4	0.0	0.0
8	4.2741983740949386E-4	0.0	0.0
9	4.2741983740949386E-4	0.0	0.0
10-14	0.001453227447192279	0.0	0.0
15-19	0.0017096793496379757	0.0	0.0
20-24	0.002137099187047469	0.0	0.0
25-29	0.002137099187047469	0.0	0.0
30-34	0.0031629067968302546	0.0	0.0
35-39	0.004017746471649242	0.0	0.0
40-44	0.004616134244022534	0.0	0.0
45-49	0.00624032962617861	0.0	0.0
50-54	0.009916140227900258	0.0	0.0
55-59	0.01239517528487532	0.0	0.0
60-64	0.017695181268753045	0.0	0.0
65-69	0.027782289431617098	0.0	0.0
70-74	0.04385327531821408	0.0	0.0
75-79	0.06556620305861635	0.0	0.0
80-84	0.0966823672220275	0.0	0.0
85-89	0.14292919362973472	0.0	0.0
90-94	0.21294056299740985	0.0	0.0
95-99	0.2916712970482386	0.0	0.0
100-104	0.38254075448149705	0.0	0.0
105-109	0.5008505654764449	0.0	0.0
110-114	0.667971721903557	0.0	0.0
115-119	0.8553525786238791	0.0	0.0
120-124	1.0813721886460193	0.0	0.0
125-129	1.345261196262641	0.0	0.0
130-134	1.6621502637180394	0.0	0.0
135-139	2.0146006616459085	0.0	0.0
140-144	2.3794462348586523	0.0	0.0
145-149	2.8063531684632546	0.0	0.0
150-154	3.283952094784623	0.0	0.0
155-159	3.831818842376112	0.0	0.0
160-164	4.416272728049854	0.0	0.0
165-169	5.058428291773877	0.0	0.0
170-174	5.740846804181876	0.0	0.0
175-179	6.466605688103195	0.0	0.0
180-184	7.253400124806592	0.0	0.0
185-189	8.154059206195878	0.0	0.0
190-194	9.152682914319419	0.0	0.0
195-199	10.08915977808362	0.0	0.0
200-204	11.075046375052358	0.0	0.0
205-209	12.142741128901275	0.0	0.0
210-214	13.271984339337157	0.0	0.0
215-219	14.380369461707456	0.0	0.0
220-224	15.5943272839179	0.0	0.0
225-229	16.90932715569195	0.0	0.0
230-234	18.237149622588284	0.0	0.0
235-239	19.568049512313966	0.0	0.0
240-244	20.87783486207162	0.0	0.0
245-249	22.140774997649192	0.0	0.0
250-254	23.55237175267779	0.0	0.0
255-259	24.894555526111073	0.0	0.0
260-264	26.209213462015203	0.0	0.0
265-269	27.40855352578624	0.0	0.0
270-274	28.541985450628733	0.0	0.0
275-279	29.583522110428184	0.0	0.0
280-284	30.544960292697102	0.0	0.0
285-289	31.455279062411847	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCTGG	10	8.460726E-4	295.0	295
CGGGTTA	3980	0.0	293.14697	1
GGGTTAT	4005	0.0	291.68542	2
GGGGGGA	2360	0.0	288.125	1
GTTATTA	4055	0.0	287.725	4
GGTTATT	4070	0.0	286.6646	3
TTATTAG	4160	0.0	280.10815	5
TTAGTAG	4190	0.0	278.45465	8
TAGTAGA	4195	0.0	278.12277	9
GGGGAGC	2445	0.0	275.6953	3
TATTAGT	4320	0.0	270.07523	6
ATTAGTA	4350	0.0	268.21265	7
AAACGTT	165	0.0	268.18182	295
GGGAGCG	1770	0.0	264.1667	4
GGAGCGA	1790	0.0	261.21506	5
CGAAAGC	1820	0.0	256.90933	9
GCGAAAG	1950	0.0	239.78206	8
AGCGAAA	2010	0.0	233.3582	7
GAGCGAA	2005	0.0	233.20448	6
GGGGGAG	2990	0.0	226.92307	2
>>END_MODULE