FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_30291_flu.3510000003ba0f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_30291_flu.3510000003ba0f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233962
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC90213.8557543532710437No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT69512.970995289833392No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG53732.2965267864012104No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA45031.9246715278549507No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA41681.7814858823227704No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG41411.769945546712714No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT33211.419461280036929No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA26911.1501867824689478No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA25051.070686692710782No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT24161.032646327181337No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT16530.7065249912378934No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA16120.6890007779041041No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT14640.625742641967499No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG13550.5791538796898641No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG10570.451782768141835No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT8300.3547584650498799No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA7990.34150845009018554No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT6760.28893581008881786No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5610.23978252878672607No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA5520.23593575025004063No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG4480.19148408715945323No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT4140.17695181268753046No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT3000.12822595122284816TruSeq Adapter, Index 7 (97% over 36bp)
GGGAGCAAAAGCTATACTAAGAAAAGCAACCAGAAGATTGGTTCAACTCA2360.10087108162864056No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGG108.460726E-4295.0295
CGGGTTA39800.0293.146971
GGGTTAT40050.0291.685422
GGGGGGA23600.0288.1251
GTTATTA40550.0287.7254
GGTTATT40700.0286.66463
TTATTAG41600.0280.108155
TTAGTAG41900.0278.454658
TAGTAGA41950.0278.122779
GGGGAGC24450.0275.69533
TATTAGT43200.0270.075236
ATTAGTA43500.0268.212657
AAACGTT1650.0268.18182295
GGGAGCG17700.0264.16674
GGAGCGA17900.0261.215065
CGAAAGC18200.0256.909339
GCGAAAG19500.0239.782068
AGCGAAA20100.0233.35827
GAGCGAA20050.0233.204486
GGGGGAG29900.0226.923072
Produced by FastQC (version 0.11.2)