##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_30191_flu.3510000003b9d7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	280371
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.788972468621935	34.0	34.0	34.0	34.0	34.0
2	33.8771128255062	34.0	34.0	34.0	34.0	34.0
3	33.88490607088465	34.0	34.0	34.0	34.0	34.0
4	33.89226061183218	34.0	34.0	34.0	34.0	34.0
5	33.89144383691609	34.0	34.0	34.0	34.0	34.0
6	37.674192409343334	38.0	38.0	38.0	38.0	38.0
7	37.67958526381116	38.0	38.0	38.0	38.0	38.0
8	37.67291909648287	38.0	38.0	38.0	38.0	38.0
9	37.591402106494606	38.0	38.0	38.0	37.0	38.0
10-14	37.672468978603355	38.0	38.0	38.0	38.0	38.0
15-19	37.64164910065592	38.0	38.0	38.0	37.6	38.0
20-24	37.63422750569781	38.0	38.0	38.0	37.6	38.0
25-29	37.53817406222469	38.0	38.0	38.0	37.0	38.0
30-34	37.48525489440777	38.0	38.0	38.0	37.0	38.0
35-39	37.39606093354876	38.0	38.0	38.0	37.0	38.0
40-44	37.40113492479607	38.0	38.0	38.0	37.0	38.0
45-49	37.367733467441354	38.0	38.0	38.0	37.0	38.0
50-54	37.33410730781715	38.0	38.0	38.0	37.0	38.0
55-59	37.28798556198751	38.0	38.0	38.0	37.0	38.0
60-64	37.121670928876384	38.0	38.0	38.0	36.6	38.0
65-69	37.05646589697223	38.0	38.0	38.0	36.4	38.0
70-74	37.132146334677984	38.0	38.0	38.0	37.0	38.0
75-79	37.05310178299467	38.0	38.0	38.0	36.8	38.0
80-84	36.96221934508205	38.0	38.0	38.0	36.2	38.0
85-89	36.99779078435359	38.0	38.0	38.0	36.0	38.0
90-94	36.849055715462725	38.0	38.0	38.0	35.8	38.0
95-99	36.85609781325458	38.0	38.0	38.0	36.0	38.0
100-104	36.83496866651687	38.0	38.0	38.0	36.0	38.0
105-109	36.6906534555999	38.0	38.0	38.0	35.2	38.0
110-114	36.60556048949428	38.0	38.0	38.0	34.8	38.0
115-119	36.70419836573682	38.0	38.0	38.0	35.0	38.0
120-124	36.61256834694031	38.0	37.8	38.0	34.8	38.0
125-129	36.51411165919443	38.0	37.0	38.0	34.0	38.0
130-134	36.39784214487232	38.0	37.0	38.0	34.0	38.0
135-139	36.17008321117376	38.0	37.0	38.0	33.6	38.0
140-144	36.12106886946225	38.0	37.0	38.0	33.4	38.0
145-149	35.7175135802205	38.0	37.0	38.0	30.6	38.0
150-154	35.364331546415286	38.0	36.4	38.0	28.4	38.0
155-159	35.424973338897395	38.0	36.0	38.0	29.0	38.0
160-164	35.40557903634827	38.0	36.0	38.0	28.6	38.0
165-169	35.20870560792664	38.0	36.0	38.0	28.2	38.0
170-174	35.06439182369075	38.0	35.4	38.0	28.0	38.0
175-179	34.680879976887766	38.0	35.0	38.0	25.0	38.0
180-184	34.40773974483809	37.6	35.0	38.0	23.8	38.0
185-189	33.6472359837501	37.0	33.8	37.8	22.8	38.0
190-194	32.67678682888031	36.8	31.2	37.0	21.0	38.0
195-199	32.43045322091086	36.8	30.4	37.2	20.4	37.8
200-204	32.2024674449212	37.0	30.0	37.0	19.8	37.4
205-209	31.165764647556273	35.4	28.2	37.0	17.8	37.2
210-214	30.425285068712526	34.6	26.4	37.0	16.0	37.0
215-219	29.583566774024415	33.4	24.6	37.0	13.8	37.0
220-224	28.10620570601096	31.6	22.0	36.2	9.4	37.0
225-229	27.11696430800618	30.4	20.0	36.2	9.0	37.0
230-234	26.728506157912193	29.6	20.0	35.8	9.0	37.0
235-239	25.653608254776707	28.2	18.4	34.6	8.8	37.0
240-244	24.570304346740567	26.6	16.6	33.8	8.2	37.0
245-249	23.692754956825063	25.0	15.2	33.0	8.0	36.2
250-254	22.821326028726226	23.8	14.0	31.8	8.0	36.0
255-259	22.16270156328579	22.4	13.2	31.0	8.0	36.0
260-264	20.88454298055077	20.6	11.6	29.4	7.8	35.2
265-269	20.100342046788008	19.6	10.2	28.6	7.0	34.0
270-274	19.133667176705153	18.6	9.0	27.0	7.0	33.4
275-279	17.86655039215896	17.0	8.0	25.0	7.0	31.8
280-284	17.57470209115779	16.4	8.0	24.6	7.0	31.0
285-289	16.397074590453364	14.6	8.0	22.6	7.0	29.4
290-294	15.77379472199336	13.2	8.0	21.4	7.0	28.6
295-299	14.950911470872523	13.0	8.0	20.2	7.0	27.0
300-301	13.155506453948519	10.5	8.0	16.5	7.0	23.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.029059314082005017
1101	2	0.057690065322667294
1101	3	0.05067231599212363
1101	4	0.006094151491005562
1101	5	0.05694758696947133
1101	6	0.1651486764369281
1101	7	0.17180881167934103
1101	8	0.186856507683558
1101	9	0.14727413093580566
1101	10-14	0.12051035428176249
1101	15-19	0.10124893531826729
1101	20-24	0.07443406975455957
1101	25-29	0.17127579618465205
1101	30-34	0.1643628069983052
1101	35-39	0.21116699191455268
1101	40-44	0.1965992703541204
1101	45-49	0.19284488544227685
1101	50-54	0.1756032098785667
1101	55-59	0.2039744815182516
1101	60-64	0.2834047619106883
1101	65-69	0.4159700296694524
1101	70-74	0.3036406300960195
1101	75-79	0.24404076419780552
1101	80-84	0.25049288292407823
1101	85-89	0.3256670446800243
1101	90-94	0.4070044300299074
1101	95-99	0.2946362945825385
1101	100-104	0.3004670204283997
1101	105-109	0.26666879174506875
1101	110-114	0.2897336589145212
1101	115-119	0.22095690426466774
1101	120-124	0.3700393848064323
1101	125-129	0.34936602350638424
1101	130-134	0.28744215929533823
1101	135-139	0.2999701401674173
1101	140-144	0.30299453213048366
1101	145-149	0.5612822101046149
1101	150-154	0.9146334932977069
1101	155-159	0.5125890740833015
1101	160-164	0.5900843955591952
1101	165-169	0.479474206302676
1101	170-174	0.5084961244220594
1101	175-179	0.6458729879479961
1101	180-184	0.11013472691832504
1101	185-189	0.4786179739934795
1101	190-194	0.8171857476508109
1101	195-199	0.39039706488782144
1101	200-204	0.48889686480977446
1101	205-209	0.3111679049595182
1101	210-214	0.3205193286587473
1101	215-219	0.016716270446067938
1101	220-224	0.7029577609975917
1101	225-229	1.2446860969774747
1101	230-234	0.7383795799879422
1101	235-239	0.8965704605340186
1101	240-244	1.135762774599094
1101	245-249	1.47156793646813
1101	250-254	0.7707914764100039
1101	255-259	-0.037484396495933936
1101	260-264	0.885774480361178
1101	265-269	0.46667041444647595
1101	270-274	0.551377690704534
1101	275-279	1.0056777835238577
1101	280-284	0.23642909780872756
1101	285-289	0.6316189515713972
1101	290-294	0.3733360935808996
1101	295-299	0.0720737857649727
1101	300-301	0.07961266366551278
1102	1	0.017936787729503578
1102	2	0.021520990951344743
1102	3	-0.028860283564554834
1102	4	0.018467078189772224
1102	5	-0.00265839025287562
1102	6	0.020566011910439386
1102	7	0.03464417054198776
1102	8	6.664136684193522E-4
1102	9	0.02235056600421359
1102	10-14	0.035775201793796896
1102	15-19	-0.018978841185585793
1102	20-24	0.034954592570421994
1102	25-29	-0.02716645712116872
1102	30-34	0.01078297056934474
1102	35-39	0.010841760698468761
1102	40-44	-0.06336480516372944
1102	45-49	0.06732436262641528
1102	50-54	0.029355407386780996
1102	55-59	-0.005993797928368849
1102	60-64	0.13859164565294435
1102	65-69	0.151391569071734
1102	70-74	0.08454906088405068
1102	75-79	-0.12599630681036444
1102	80-84	0.06648539228737604
1102	85-89	0.058124049954038526
1102	90-94	0.21651257036469218
1102	95-99	0.08201954001553702
1102	100-104	0.15830237929105095
1102	105-109	0.05715071485138168
1102	110-114	0.28175804170134455
1102	115-119	0.05935635393217353
1102	120-124	0.08804137211821228
1102	125-129	0.16380766533166025
1102	130-134	0.30057714362614973
1102	135-139	0.24201095649395654
1102	140-144	0.27855671205863786
1102	145-149	0.32517199073597425
1102	150-154	0.7956217987322418
1102	155-159	0.618335080350974
1102	160-164	0.39626302431406657
1102	165-169	0.45607437445982413
1102	170-174	0.3649720855930454
1102	175-179	0.3418559429277437
1102	180-184	0.6363404901224854
1102	185-189	0.6071790838306796
1102	190-194	0.7399591941080459
1102	195-199	0.7133512802818487
1102	200-204	0.13457102211314265
1102	205-209	-0.07550564086330169
1102	210-214	-0.16165677773886244
1102	215-219	0.011863025777408609
1102	220-224	0.9042219970290866
1102	225-229	0.9669806777311294
1102	230-234	0.739646109071483
1102	235-239	1.2384714006853663
1102	240-244	1.1269073897485313
1102	245-249	1.5046941307660724
1102	250-254	0.649854382471311
1102	255-259	0.5562613040314695
1102	260-264	1.327572615345506
1102	265-269	1.0825994827029923
1102	270-274	1.0603760091330336
1102	275-279	1.7545786472401268
1102	280-284	0.7011741868555816
1102	285-289	0.9822499230611186
1102	290-294	0.9805653223510582
1102	295-299	1.1769102147185766
1102	300-301	0.9416530978166993
1103	1	-0.10354641763054673
1103	2	0.02306259956063883
1103	3	0.03171085277886476
1103	4	-0.022678307360607164
1103	5	0.035099903304981694
1103	6	-0.0024728840606229596
1103	7	-0.012015430418557571
1103	8	-0.15408366785895566
1103	9	0.09443188585835571
1103	10-14	0.015406698043534561
1103	15-19	0.004945738577838199
1103	20-24	0.04555463407527327
1103	25-29	-0.10195522709537386
1103	30-34	-0.0016677424246367423
1103	35-39	-0.7204536352281252
1103	40-44	-0.40116534472674914
1103	45-49	-0.33146829101563924
1103	50-54	-0.0957313080974771
1103	55-59	-0.4755803800042031
1103	60-64	-0.028063656591271524
1103	65-69	0.11726272616758138
1103	70-74	-0.0013815370822101158
1103	75-79	0.05811252482818219
1103	80-84	0.06475849418177404
1103	85-89	0.08225096584742886
1103	90-94	0.13976195520356782
1103	95-99	0.06849266562699086
1103	100-104	0.16170874139119462
1103	105-109	-0.037847209906253454
1103	110-114	0.16503529408046091
1103	115-119	-0.11484838354967053
1103	120-124	0.18716680569563238
1103	125-129	0.2933205763037563
1103	130-134	0.2843391133276043
1103	135-139	0.29794763195568663
1103	140-144	0.16183322327977123
1103	145-149	0.4814807275063089
1103	150-154	0.7372407842351905
1103	155-159	-0.0046815808805362735
1103	160-164	-0.5144000156195219
1103	165-169	0.572430248878014
1103	170-174	0.6842073587838868
1103	175-179	0.3987227716609567
1103	180-184	0.316653851718641
1103	185-189	0.35205397636276103
1103	190-194	0.25041174517383524
1103	195-199	0.22371711586336573
1103	200-204	0.26367259040345203
1103	205-209	0.514217462513411
1103	210-214	0.47079556580268545
1103	215-219	0.15874200950158368
1103	220-224	0.861400853274084
1103	225-229	1.76701625331442
1103	230-234	0.896292813883079
1103	235-239	0.7569484692572992
1103	240-244	1.155426407862425
1103	245-249	1.344937527048426
1103	250-254	1.0991651055450404
1103	255-259	0.3836937845390125
1103	260-264	1.2895208232254944
1103	265-269	0.8018399439997239
1103	270-274	1.0492578981965046
1103	275-279	1.6935176054684646
1103	280-284	0.8893334468834482
1103	285-289	0.4384141340243275
1103	290-294	0.9623239420184291
1103	295-299	0.9604068498613216
1103	300-301	-0.7604569789672677
2116	1	-0.019636762923518347
2116	2	-0.02173515537454307
2116	3	0.006400317612254014
2116	4	0.036379608028155985
2116	5	0.04781408241745311
2116	6	-0.17357160193152055
2116	7	-0.07496150673219404
2116	8	0.04290577448093558
2116	9	0.13514153770906745
2116	10-14	0.09970231252648176
2116	15-19	0.10920379249794365
2116	20-24	-0.17942028389489195
2116	25-29	-0.16805681913481862
2116	30-34	0.05238983377653739
2116	35-39	-0.06888676707212937
2116	40-44	-0.12344675617557499
2116	45-49	0.12496716872636426
2116	50-54	0.22978416778270372
2116	55-59	0.13207883286636246
2116	60-64	0.34035762153897764
2116	65-69	0.2547015385111493
2116	70-74	-0.8055214109044257
2116	75-79	0.1378141111549951
2116	80-84	-0.017021776525623977
2116	85-89	0.11023858371512318
2116	90-94	0.20736454159822415
2116	95-99	0.16451524480944357
2116	100-104	0.2446033325987358
2116	105-109	0.256979286714369
2116	110-114	0.41918677168872875
2116	115-119	0.3546886432390082
2116	120-124	-0.06599460118309963
2116	125-129	0.10585659267044889
2116	130-134	0.14066281968398187
2116	135-139	0.408722729159706
2116	140-144	0.49807595306150887
2116	145-149	0.6147882349773539
2116	150-154	0.860844313214308
2116	155-159	-0.4149725324266953
2116	160-164	-0.16647245180762837
2116	165-169	0.8688748488330305
2116	170-174	0.8476122309458631
2116	175-179	0.8137991245731442
2116	180-184	0.7389731093619645
2116	185-189	0.9198286558304929
2116	190-194	0.8917719231223558
2116	195-199	0.8757077899897752
2116	200-204	0.6797493681927236
2116	205-209	0.6160126574748759
2116	210-214	0.8769491428036211
2116	215-219	1.1383743301516809
2116	220-224	1.6368990127350997
2116	225-229	1.0181111151579536
2116	230-234	1.4078973140098334
2116	235-239	1.4333984962137905
2116	240-244	1.836499383180282
2116	245-249	1.0361297178789357
2116	250-254	1.047547894809572
2116	255-259	0.8133841241193522
2116	260-264	0.1933322206426702
2116	265-269	0.54689833782151
2116	270-274	0.7526351354957157
2116	275-279	1.4795220113367655
2116	280-284	0.2694352745690125
2116	285-289	-1.004516660995682
2116	290-294	0.4233455750460138
2116	295-299	0.6832066827668015
2116	300-301	1.3672757126469453
1117	1	-0.007329009056768143
1117	2	0.07070849850239114
1117	3	0.03674319595700837
1117	4	0.07310644027748481
1117	5	0.03888745969275931
1117	6	-0.3408421069732128
1117	7	-0.2966956868942816
1117	8	-0.03438553752162221
1117	9	0.05765925272645234
1117	10-14	0.1423536722541101
1117	15-19	0.1211776164681524
1117	20-24	0.08828309323492078
1117	25-29	0.20672565354695394
1117	30-34	0.10585062793312972
1117	35-39	-0.133872507756287
1117	40-44	-0.10233423853330947
1117	45-49	0.039533496370523835
1117	50-54	0.028277118151564196
1117	55-59	0.153511457598988
1117	60-64	0.2573272986673274
1117	65-69	0.3011861806459706
1117	70-74	-0.07936902537907997
1117	75-79	0.18909672206483208
1117	80-84	0.014793958192889534
1117	85-89	0.058592214748053095
1117	90-94	0.26520170894147554
1117	95-99	0.3413314887892511
1117	100-104	0.22965069389778847
1117	105-109	0.22653273732329637
1117	110-114	0.2473252762066025
1117	115-119	0.23515374429100433
1117	120-124	0.3735131614138396
1117	125-129	0.30669760129879364
1117	130-134	0.17426024136687346
1117	135-139	0.2511507414401848
1117	140-144	0.13864188784697262
1117	145-149	0.7115740687835768
1117	150-154	0.903964193319652
1117	155-159	0.10095861544822782
1117	160-164	-0.6983092844934689
1117	165-169	0.1852214084093191
1117	170-174	0.5206806756619073
1117	175-179	0.8379686649284679
1117	180-184	0.5315742704776838
1117	185-189	0.6328828412306962
1117	190-194	1.0687558595665436
1117	195-199	0.6257470758599553
1117	200-204	0.7612624562076249
1117	205-209	1.1527413085144858
1117	210-214	0.7561425456874993
1117	215-219	0.5027202204131456
1117	220-224	1.3679588582106739
1117	225-229	0.7717861628102618
1117	230-234	0.9750605104246333
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2108	165-169	-0.1717678389025039
2108	170-174	-0.9005558834778711
2108	175-179	-0.9623001522758301
2108	180-184	-0.7100923961889833
2108	185-189	-1.253138664145638
2108	190-194	-0.8992548931216291
2108	195-199	-1.5688765800540274
2108	200-204	-0.9959956083085117
2108	205-209	-0.5913447129908818
2108	210-214	-0.11858863710819989
2108	215-219	-0.9421722527051308
2108	220-224	-0.6411809267355615
2108	225-229	-0.20832136407145896
2108	230-234	-0.25182121000547397
2108	235-239	-0.6577832809625797
2108	240-244	-0.6407831986289025
2108	245-249	-0.014083808100661344
2108	250-254	-0.13958925652832832
2108	255-259	-0.535224137553648
2108	260-264	-0.2883631101589046
2108	265-269	-1.1432099893279357
2108	270-274	-0.6923846529426001
2108	275-279	-0.013429085030018939
2108	280-284	-0.4753571650835262
2108	285-289	-0.03045697557365301
2108	290-294	-0.41536919498613933
2108	295-299	-0.04128478346917319
2108	300-301	-0.1146928004556198
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	10.0
13	68.0
14	223.0
15	330.0
16	371.0
17	440.0
18	818.0
19	742.0
20	706.0
21	859.0
22	1337.0
23	2082.0
24	3031.0
25	4522.0
26	6214.0
27	8578.0
28	11743.0
29	16276.0
30	22966.0
31	32433.0
32	43965.0
33	53698.0
34	47215.0
35	19930.0
36	1813.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.875921546807625	23.92686832803678	16.588734212882216	31.60847591227338
2	40.142882109775975	25.355689425796534	18.782613037725014	15.718815426702475
3	39.27795670736274	24.328835721240786	20.77532983083129	15.61787774056518
4	40.011984121039625	23.811307160868992	20.358025616058722	15.818683102032663
5	27.250678565186842	23.86159766880312	33.33476001440948	15.552963751600558
6	25.863231218635306	23.689682599127586	33.8947323367966	16.552353845440507
7	18.188757039779436	44.16398272289217	21.59032139557943	16.05693884174897
8	25.393853144583428	22.319712095758835	35.11097795421067	17.175456805447066
9	16.65150818023262	20.66654539877519	36.757367916082615	25.92457850490957
10-14	25.4092613002058	33.27469674110375	24.096429373936676	17.219612584753776
15-19	21.378459255771816	34.16508840072618	22.818907804302157	21.637544539199848
20-24	24.491120693652338	30.4077811185893	26.617303501432033	18.483794686326334
25-29	19.825873574656438	26.103840982127252	35.722524797500455	18.347760645715855
30-34	19.937297366703405	28.45843543019784	30.350927877704898	21.253339325393853
35-39	20.999176091678525	29.82070185575541	27.78247393632009	21.397648116245975
40-44	20.727179344511377	28.874027627679038	28.819385742462668	21.579407285346917
45-49	21.189424013182535	30.060455610601664	26.7561909041948	21.993929472021
50-54	22.104497255422277	29.20487496923719	27.640091164920765	21.050536610419766
55-59	21.524694066076734	27.19182797079584	29.464316922934252	21.819161040193173
60-64	21.699890502227408	26.80962011049645	28.532622846157413	22.957866541118733
65-69	20.636442428068523	27.568329106790646	29.040877979534258	22.754350485606572
70-74	21.28593898798378	26.82167556558988	28.977390671645786	22.91499477478056
75-79	20.84916057652182	26.366778304460876	30.114598157441392	22.669462961575913
80-84	23.47917580634231	25.749738739027933	27.59693406236736	23.174151392262395
85-89	21.06722877901067	28.650324034939416	26.606104054984286	23.676343131065625
90-94	20.608693481137493	30.11145945907387	28.499880515459875	20.779966544328765
95-99	21.871591569741522	30.017084505886842	26.637633706767105	21.47369021760453
100-104	22.452464769894178	28.79755752199764	26.591480573953795	22.15849713415439
105-109	23.386869540715693	27.83882783882784	24.686861337299508	24.08744128315696
110-114	21.692257758470028	28.052473329980632	28.85647945044245	21.39878946110689
115-119	23.087837187155593	27.97265052377029	27.01320750006242	21.926304789011702
120-124	19.658095880101722	28.522850080785815	28.096700443341145	23.722353595771317
125-129	21.25612135349234	25.986068459291438	29.774976727264946	22.98283345995128
130-134	24.211633870835428	28.616012355058118	26.0004066041067	21.17194716999975
135-139	23.558499274176004	27.82941174372528	25.327655142650272	23.284433839448447
140-144	21.25797603889133	29.345617057398947	26.702476361677917	22.693930542031808
145-149	20.66426270905336	27.73139875379408	28.61265965452918	22.991678882623383
150-154	22.879256413823114	26.46043991710983	26.052979801762667	24.60732386730439
155-159	21.94050026571935	27.59029999536329	28.96726123600515	21.501938502912214
160-164	23.909106148638767	29.076545006437897	26.476632747324082	20.53771609759925
165-169	20.237970403501077	26.4474571193169	28.168747837686492	25.145824639495522
170-174	21.83599587689169	28.99950422832604	26.78572320247101	22.37877669231126
175-179	21.015297587838973	28.83029985269518	28.066740140742084	22.087662418723763
180-184	20.182401175585206	29.491352529327212	26.88181017294941	23.444436122138168
185-189	22.03851325565055	27.919863323952903	27.601570775864836	22.44005264453171
190-194	20.20829543711725	28.2550620427933	27.562337046270834	23.974305473818617
195-199	20.985551287401336	29.762992606225325	27.417243580826835	21.83421252554651
200-204	21.85197470494452	28.262766120604482	27.453409946107122	22.43184922834387
205-209	21.190422689935833	28.05575469645577	27.810008881089697	22.9438137325187
210-214	22.333122897874603	28.249355318488718	27.37251712908967	22.04500465454701
215-219	22.075892299845563	27.8688594754807	27.850241287437004	22.205006937236732
220-224	19.502730310909474	27.878988911121333	27.872711514386296	24.745569263582894
225-229	20.93155140866923	28.99921889211081	26.50659304992314	23.562636649296824
230-234	20.760349679531764	27.881057598681746	27.87549354248478	23.48309917930171
235-239	21.111384558317372	28.0884256930995	26.280321431246456	24.51986831733667
240-244	22.230615862553545	31.274917876670553	23.7760681382882	22.718398122487706
245-249	20.268929382853436	29.774834059157328	24.833024813550615	25.123211744438617
250-254	20.833039080361377	28.09798445630968	25.55200074187416	25.516975721454788
255-259	21.1116698945326	28.526630785637604	26.516080479079505	23.84561884075029
260-264	20.830043050101473	29.57131800364517	25.960031529651783	23.638607416601573
265-269	20.005064717820318	28.96690456573611	26.209843386084863	24.818187330358704
270-274	19.245499712880434	30.971676813935822	24.737508515502675	25.045314957681075
275-279	20.091378922927124	30.004529712416762	23.68112251267071	26.222968851985407
280-284	17.913193589921924	30.75232459847844	26.207846032578264	25.126635779021367
285-289	19.703393004269344	29.231553905361114	26.510873093151577	24.55417999721797
290-294	19.09198882908717	31.449329638229344	25.14496863084984	24.313712901833643
295-299	18.492069436567977	32.451002421791124	22.935039643900403	26.121888497740493
300-301	14.51594494437727	35.63563991996319	27.242296813864485	22.606118321795048
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.25
30	0.25
31	0.75
32	3.25
33	4.0
34	13.25
35	49.75
36	140.0
37	470.25
38	1494.0
39	2686.75
40	4827.0
41	6638.75
42	10152.0
43	12688.5
44	13762.75
45	10619.5
46	7406.25
47	6138.75
48	5438.75
49	4376.5
50	3481.75
51	1670.5
52	472.5
53	145.25
54	102.5
55	93.25
56	97.25
57	91.0
58	83.25
59	103.25
60	90.0
61	39.75
62	16.0
63	6.25
64	2.75
65	2.0
66	0.75
67	1.5
68	1.25
69	1.5
70	0.25
71	0.25
72	0.5
73	0.75
74	0.25
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.25
82	0.25
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	280371.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.9635244901429
#Duplication Level	Percentage of deduplicated	Percentage of total
1	38.31605903235389	4.967111696316807
2	7.43988958662001	1.928943817202153
3	5.119425662677431	1.9909739986075474
4	4.952525744050476	2.568087550842461
5	4.5185912910209725	2.92884344310484
6	4.566820719066192	3.5521255340223954
7	4.293692168908016	3.896296850934169
8	4.523785475267312	4.691536303742443
9	3.4309897856499703	4.002994810053318
>10	22.48230269691958	46.952268648523976
>50	0.259326711043551	2.130114252187466
>100	0.038684055700640034	0.9913515525974301
>500	0.016544877349219795	1.573567699562221
>1k	0.03584723425647564	12.339029953047248
>5k	0.0055149591163808675	5.486753889255525
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	8431	3.007087038245753	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	6918	2.4674449211937044	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	4792	1.709163929222352	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	4553	1.6239197349226562	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	4278	1.5258354109376504	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	3504	1.2497726227034893	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	2999	1.0696541368401153	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	2559	0.9127192184641065	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	2309	0.8235516512050105	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2136	0.7618476946617161	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1723	0.6145428735496895	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	1715	0.6116895113973985	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1676	0.5977793709049795	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1251	0.4461945065645163	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1023	0.36487368522422076	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	954	0.34026343666071024	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	913	0.3256399556302185	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT	834	0.2974630043763442	TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	603	0.21507217222893948	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	594	0.21186213980761204	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	504	0.1797618155943375	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	469	0.16727835617806405	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	408	0.14552146976684466	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	293	0.10450438882766049	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	3.5667026903638395E-4	0.0	0.0
2	7.133405380727679E-4	0.0	0.0
3	7.133405380727679E-4	0.0	0.0
4	7.133405380727679E-4	0.0	0.0
5	7.133405380727679E-4	0.0	0.0
6	0.0010700108071091518	0.0	0.0
7	0.0010700108071091518	0.0	0.0
8	0.0014266810761455358	0.0	0.0
9	0.0017833513451819196	0.0	0.0
10-14	0.003281366475134732	0.0	0.0
15-19	0.004066041067014777	0.0	0.0
20-24	0.005350054035545759	0.0	0.0
25-29	0.005350054035545759	0.0	0.0
30-34	0.006776735111691296	0.0	0.0
35-39	0.007347407542149509	0.0	0.0
40-44	0.009130758887331428	0.0	0.0
45-49	0.011698784824393392	0.0	0.0
50-54	0.017904847505626473	0.0	0.0
55-59	0.026179597747270578	0.0	0.0
60-64	0.035096354473180186	0.0	0.0
65-69	0.048435822535140935	0.0	0.0
70-74	0.07219006245296411	0.0	0.0
75-79	0.1026497034286713	0.0	0.0
80-84	0.14701948489679745	0.0	0.0
85-89	0.21150546953857569	0.0	0.0
90-94	0.30081570490528625	0.0	0.0
95-99	0.40539142778675397	0.0	0.0
100-104	0.5286566727657283	0.0	0.0
105-109	0.681668218182337	0.0	0.0
110-114	0.847876563553292	0.0	0.0
115-119	1.0526052979801763	0.0	0.0
120-124	1.2984937814538593	0.0	0.0
125-129	1.5663531535001838	0.0	0.0
130-134	1.8800089880907798	0.0	0.0
135-139	2.2211284333971775	0.0	0.0
140-144	2.598057573714828	0.0	0.0
145-149	3.010439738774695	0.0	0.0
150-154	3.4441507859229383	0.0	0.0
155-159	3.9454865160804786	0.0	0.0
160-164	4.516729618969152	0.0	0.0
165-169	5.162088803763585	0.0	0.0
170-174	5.877712031558186	0.0	0.0
175-179	6.661102610469699	0.0	0.0
180-184	7.513758555628078	0.0	0.0
185-189	8.470419551237468	0.0	0.0
190-194	9.504406661173944	0.0	0.0
195-199	10.594319669295327	0.0	0.0
200-204	11.766052837133655	0.0	0.0
205-209	13.093222908218042	0.0	0.0
210-214	14.457272685120785	0.0	0.0
215-219	15.834447927924074	0.0	0.0
220-224	17.337670443804814	0.0	0.0
225-229	18.892895484911065	0.0	0.0
230-234	20.515745209026612	0.0	0.0
235-239	22.150935724450818	0.0	0.0
240-244	23.74896119784143	0.0	0.0
245-249	25.274083268241007	0.0	0.0
250-254	26.940946103555643	0.0	0.0
255-259	28.512863313252797	0.0	0.0
260-264	30.07900246459156	0.0	0.0
265-269	31.488848704038578	0.0	0.0
270-274	32.801751964361515	0.0	0.0
275-279	33.99838071697857	0.0	2.1400216142183036E-4
280-284	35.07288556947758	0.0	3.5667026903638395E-4
285-289	36.06435758334492	0.0	3.5667026903638395E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	3235	0.0	293.63214	1
GGGTTAT	3265	0.0	290.93414	2
GGTTATT	3330	0.0	285.6982	3
GGGGGGA	2425	0.0	285.26804	1
GTTATTA	3355	0.0	283.56927	4
TTAGTAG	3490	0.0	272.60028	8
GGGGAGC	2530	0.0	272.26285	3
GGGAGCG	1855	0.0	271.9407	4
GGAGCGA	1875	0.0	269.04	5
TTATTAG	3565	0.0	267.27908	5
TATTAGT	3615	0.0	263.1743	6
CGAAAGC	1920	0.0	261.1979	9
ATTAGTA	3660	0.0	259.93854	7
TAGTAGA	3685	0.0	258.17505	9
GCGAAAG	2115	0.0	239.20804	8
GAGCGAA	2120	0.0	237.9481	6
AGCGAAA	2190	0.0	230.34247	7
GGGAGCA	815	0.0	228.0368	4
GGGGGAG	3195	0.0	215.59468	2
AACCGTT	30	4.663998E-7	196.66666	295
>>END_MODULE