FastQCFastQC Report
Fri 30 Oct 2015
000000000-AJTU1_l01n01_30191_flu.3510000003b9d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTU1_l01n01_30191_flu.3510000003b9d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280371
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC84313.007087038245753No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT69182.4674449211937044No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA47921.709163929222352No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG45531.6239197349226562No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG42781.5258354109376504No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA35041.2497726227034893No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT29991.0696541368401153No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT25590.9127192184641065No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA23090.8235516512050105No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT21360.7618476946617161No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT17230.6145428735496895No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA17150.6116895113973985No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG16760.5977793709049795No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA12510.4461945065645163No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT10230.36487368522422076No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT9540.34026343666071024No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG9130.3256399556302185No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT8340.2974630043763442TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA6030.21507217222893948No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5940.21186213980761204No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5040.1797618155943375No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA4690.16727835617806405No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT4080.14552146976684466No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG2930.10450438882766049No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA32350.0293.632141
GGGTTAT32650.0290.934142
GGTTATT33300.0285.69823
GGGGGGA24250.0285.268041
GTTATTA33550.0283.569274
TTAGTAG34900.0272.600288
GGGGAGC25300.0272.262853
GGGAGCG18550.0271.94074
GGAGCGA18750.0269.045
TTATTAG35650.0267.279085
TATTAGT36150.0263.17436
CGAAAGC19200.0261.19799
ATTAGTA36600.0259.938547
TAGTAGA36850.0258.175059
GCGAAAG21150.0239.208048
GAGCGAA21200.0237.94816
AGCGAAA21900.0230.342477
GGGAGCA8150.0228.03684
GGGGGAG31950.0215.594682
AACCGTT304.663998E-7196.66666295
Produced by FastQC (version 0.11.2)