##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_30112_flu.3510000003bb28.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421526
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.78691468616408	34.0	34.0	34.0	34.0	34.0
2	33.870477740400354	34.0	34.0	34.0	34.0	34.0
3	33.880313432623375	34.0	34.0	34.0	34.0	34.0
4	33.88261934020677	34.0	34.0	34.0	34.0	34.0
5	33.87808818435874	34.0	34.0	34.0	34.0	34.0
6	37.62008986397043	38.0	38.0	38.0	38.0	38.0
7	37.621143179780134	38.0	38.0	38.0	38.0	38.0
8	37.63053524574997	38.0	38.0	38.0	38.0	38.0
9	37.54347774514502	38.0	38.0	38.0	37.0	38.0
10-14	37.635170784245815	38.0	38.0	38.0	37.6	38.0
15-19	37.60942338076418	38.0	38.0	38.0	37.0	38.0
20-24	37.60380427304603	38.0	38.0	38.0	37.0	38.0
25-29	37.50515934960121	38.0	38.0	38.0	37.0	38.0
30-34	37.43736044751688	38.0	38.0	38.0	37.0	38.0
35-39	37.344582777812036	38.0	38.0	38.0	37.0	38.0
40-44	37.35216333037583	38.0	38.0	38.0	37.0	38.0
45-49	37.31690619321228	38.0	38.0	38.0	37.0	38.0
50-54	37.27866228892168	38.0	38.0	38.0	37.0	38.0
55-59	37.21468663854661	38.0	38.0	38.0	37.0	38.0
60-64	37.05533466500287	38.0	38.0	38.0	36.4	38.0
65-69	36.975870527559394	38.0	38.0	38.0	36.0	38.0
70-74	37.05466709052348	38.0	38.0	38.0	36.2	38.0
75-79	36.96058985685343	38.0	38.0	38.0	36.0	38.0
80-84	36.84816167923213	38.0	38.0	38.0	35.8	38.0
85-89	36.89145295901083	38.0	38.0	38.0	36.0	38.0
90-94	36.727594501881256	38.0	38.0	38.0	35.4	38.0
95-99	36.73429491893738	38.0	38.0	38.0	35.0	38.0
100-104	36.68927325953796	38.0	38.0	38.0	35.0	38.0
105-109	36.525935292247695	38.0	38.0	38.0	34.0	38.0
110-114	36.432849219265236	38.0	37.0	38.0	34.0	38.0
115-119	36.51874332781371	38.0	37.2	38.0	34.0	38.0
120-124	36.409007748039265	38.0	37.0	38.0	34.0	38.0
125-129	36.30362112894578	38.0	37.0	38.0	34.0	38.0
130-134	36.16983531264975	38.0	37.0	38.0	33.4	38.0
135-139	35.9025198920114	38.0	37.0	38.0	31.6	38.0
140-144	35.859336316146575	38.0	37.0	38.0	31.2	38.0
145-149	35.41847335632915	38.0	36.2	38.0	28.6	38.0
150-154	35.01528161963912	38.0	35.6	38.0	27.0	38.0
155-159	35.09495831811087	38.0	35.6	38.0	27.6	38.0
160-164	35.01353890388731	38.0	35.8	38.0	27.4	38.0
165-169	34.76078960728401	38.0	35.2	38.0	25.4	38.0
170-174	34.64754866840954	38.0	35.0	38.0	24.6	38.0
175-179	34.25182740803651	37.8	34.4	38.0	23.4	38.0
180-184	33.9579086462045	37.2	34.0	38.0	23.0	38.0
185-189	33.158786883845835	37.0	32.8	37.8	21.4	38.0
190-194	32.1811219236773	36.8	29.8	37.0	20.0	38.0
195-199	31.8920488890365	36.2	29.4	37.2	19.4	37.8
200-204	31.702269373656662	36.8	29.0	37.0	19.0	37.4
205-209	30.612914031400198	35.0	26.2	37.0	16.0	37.2
210-214	29.839708108159403	33.6	24.8	37.0	14.8	37.0
215-219	29.042831047195193	32.8	23.4	36.8	12.4	37.0
220-224	27.57026660277183	31.2	21.0	36.2	9.2	37.0
225-229	26.600559396098937	29.2	20.0	35.8	9.0	37.0
230-234	26.169528332771883	28.8	19.2	35.6	9.0	37.0
235-239	25.14454719281847	27.4	17.8	33.8	8.8	37.0
240-244	24.140857266218454	25.8	16.4	33.0	8.0	37.0
245-249	23.34412301969511	24.4	15.0	32.0	8.0	36.2
250-254	22.475375184448886	23.0	13.6	31.6	8.0	36.0
255-259	21.90638394784663	22.2	13.0	30.6	8.0	36.0
260-264	20.665128604166767	20.2	11.4	29.2	7.8	34.8
265-269	19.929375174959553	19.4	10.2	28.0	7.0	34.0
270-274	19.093208485360332	18.6	9.0	26.8	7.0	33.2
275-279	17.865353026859555	17.0	8.0	25.0	7.0	31.6
280-284	17.64939197107652	16.8	8.0	24.6	7.0	30.8
285-289	16.498417653952544	14.6	8.0	22.6	7.0	29.4
290-294	15.922516286065392	13.6	8.0	21.6	7.0	28.8
295-299	15.127325004863284	13.0	8.0	20.8	7.0	27.0
300-301	13.263799860506825	11.0	8.0	16.5	7.0	23.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.1890021400265809
1101	2	0.015786643915582488
1101	3	0.03018617407062152
1101	4	0.03390622064257798
1101	5	0.07777779457652656
1101	6	0.2365327440747862
1101	7	0.1619043901360655
1101	8	0.14050066775308068
1101	9	0.12491525600847808
1101	10-14	0.09910046679947015
1101	15-19	0.15166733712882774
1101	20-24	0.12615325109530318
1101	25-29	0.11579974610895505
1101	30-34	0.17306182428333017
1101	35-39	0.21776437810105875
1101	40-44	0.2657494605328168
1101	45-49	0.2027392829906205
1101	50-54	0.1741678173919894
1101	55-59	0.22153088938442522
1101	60-64	0.3561430952067468
1101	65-69	0.3932634040676035
1101	70-74	0.33041879373786287
1101	75-79	0.359601697217407
1101	80-84	0.3520293087754567
1101	85-89	0.3696698873107209
1101	90-94	0.45731740774409246
1101	95-99	0.33723065239758654
1101	100-104	0.3847594260774869
1101	105-109	0.40519437891578747
1101	110-114	0.3831028039330704
1101	115-119	0.3630639517617027
1101	120-124	0.49185658232732266
1101	125-129	0.502906428759843
1101	130-134	0.46423577885851586
1101	135-139	0.5119769920916397
1101	140-144	0.4984851351590649
1101	145-149	0.7800328314263041
1101	150-154	0.8862558966035365
1101	155-159	0.5625213139988361
1101	160-164	0.6237708033857317
1101	165-169	0.5582734817417787
1101	170-174	0.5453697144250711
1101	175-179	0.7495497662322705
1101	180-184	0.2887175315509296
1101	185-189	0.5540736817624747
1101	190-194	0.6338958598846816
1101	195-199	0.6000515623637313
1101	200-204	0.01722272007796022
1101	205-209	-0.013760784742885335
1101	210-214	-0.26440286963450177
1101	215-219	0.09066384766214597
1101	220-224	0.6665289421207419
1101	225-229	0.6469879678607882
1101	230-234	0.301047223158303
1101	235-239	0.3893937066227693
1101	240-244	0.7320596983709997
1101	245-249	0.8754125406565194
1101	250-254	0.6595604757497178
1101	255-259	0.2777713588335864
1101	260-264	0.8959593427957984
1101	265-269	0.20270883380385385
1101	270-274	0.06468481795003811
1101	275-279	0.7549751213151019
1101	280-284	0.42505019368207186
1101	285-289	0.44121925032745324
1101	290-294	0.02135491740850526
1101	295-299	0.10934719662801484
1101	300-301	0.0684039458314114
1102	1	-0.09936178593870437
1102	2	0.01914688630039052
1102	3	0.029447361322368693
1102	4	0.02322563176137038
1102	5	0.08106601966362348
1102	6	0.07667044114800348
1102	7	0.10843281748162781
1102	8	-0.007285605241619919
1102	9	0.14115812205970002
1102	10-14	0.0939979141675309
1102	15-19	0.06959659411366204
1102	20-24	0.09035048698669357
1102	25-29	0.06869229968982893
1102	30-34	0.1324150920046776
1102	35-39	0.10691806470889276
1102	40-44	0.04480039116575796
1102	45-49	0.12406962040437008
1102	50-54	0.15237487474824718
1102	55-59	0.062110010129472926
1102	60-64	0.3146418141460998
1102	65-69	0.34716538598363655
1102	70-74	0.24239298273837306
1102	75-79	0.09780187480358649
1102	80-84	0.2524858137166959
1102	85-89	0.24889365945816877
1102	90-94	0.426439948988822
1102	95-99	0.20764645485667188
1102	100-104	0.2774995741111681
1102	105-109	0.24205910162377364
1102	110-114	0.2567085490494492
1102	115-119	0.16810838219807778
1102	120-124	0.3068971831379841
1102	125-129	0.3028181440254727
1102	130-134	0.41502186884455483
1102	135-139	0.33978041367581113
1102	140-144	0.3616116009228918
1102	145-149	0.5547037547389024
1102	150-154	1.0267874012058584
1102	155-159	0.6457846634640418
1102	160-164	0.6726271686981349
1102	165-169	0.8389620009983574
1102	170-174	0.7936267263680747
1102	175-179	0.6933776296400325
1102	180-184	0.9479235450800161
1102	185-189	1.062913339061815
1102	190-194	1.0571437213945671
1102	195-199	1.0808424309092928
1102	200-204	0.8159445401329997
1102	205-209	0.7483264290519003
1102	210-214	0.14671151755760548
1102	215-219	0.30029942319050207
1102	220-224	1.1414801398107706
1102	225-229	1.4490264810170785
1102	230-234	0.8671328373218188
1102	235-239	1.3351945050827858
1102	240-244	1.475016067750257
1102	245-249	1.3159760717747702
1102	250-254	0.8118943445528437
1102	255-259	0.9881845517247143
1102	260-264	1.6779636604446146
1102	265-269	1.06734867953433
1102	270-274	1.166273773716167
1102	275-279	1.5868686170516746
1102	280-284	0.6551144500568746
1102	285-289	0.9647441666179404
1102	290-294	1.2318594451783742
1102	295-299	1.0918551270033845
1102	300-301	1.0497061371906913
1103	1	-0.024295938704291586
1103	2	-0.035616063655716346
1103	3	0.00969319099752397
1103	4	0.03233449918894138
1103	5	0.03645451834668023
1103	6	-0.04465308826375747
1103	7	0.13669225558522413
1103	8	0.0943386265406474
1103	9	0.20003652416680495
1103	10-14	0.07503391065567655
1103	15-19	0.06131081711629349
1103	20-24	0.12275830085850714
1103	25-29	0.08421029793390034
1103	30-34	0.16537163282995238
1103	35-39	-0.28482908191094936
1103	40-44	-0.16630584237243085
1103	45-49	-0.08969245158861128
1103	50-54	0.1736215823865308
1103	55-59	-0.12138428311020988
1103	60-64	0.2290623584832403
1103	65-69	0.3237786432268379
1103	70-74	0.24063705648727307
1103	75-79	0.3855433146631171
1103	80-84	0.2626043387386474
1103	85-89	0.2693502002834549
1103	90-94	0.38472572598618626
1103	95-99	0.3879691063053201
1103	100-104	0.35913917024812747
1103	105-109	0.05803803050876155
1103	110-114	0.44777527424699315
1103	115-119	0.1474213760523284
1103	120-124	0.48187523405984933
1103	125-129	0.37284667782705583
1103	130-134	0.5372453104600083
1103	135-139	0.4889682977846732
1103	140-144	0.4874676149702992
1103	145-149	0.7368372051339804
1103	150-154	0.95045948197847
1103	155-159	0.027490106835415418
1103	160-164	-0.45058398143356015
1103	165-169	0.4130181906383896
1103	170-174	0.8260455147789614
1103	175-179	0.5278376823178164
1103	180-184	0.5280376802863316
1103	185-189	0.7905033302821778
1103	190-194	0.46818410769834173
1103	195-199	0.5467774616380012
1103	200-204	0.7381899308265893
1103	205-209	0.8490836722476836
1103	210-214	0.8805350469693671
1103	215-219	0.7017590368867239
1103	220-224	1.409061351399881
1103	225-229	1.9300844265833668
1103	230-234	1.0775037767423612
1103	235-239	1.4564960854164113
1103	240-244	1.355514311824006
1103	245-249	1.4472754572005826
1103	250-254	1.5275550118048145
1103	255-259	0.8488957018564065
1103	260-264	1.7654417113664778
1103	265-269	0.49260109393292595
1103	270-274	1.089923905410636
1103	275-279	1.6765635248655464
1103	280-284	0.8114357845608247
1103	285-289	0.8942327530973024
1103	290-294	1.1194472414909296
1103	295-299	1.0910539856513246
1103	300-301	-0.7998054958207224
2116	1	0.018492591547740744
2116	2	0.01846269544785173
2116	3	0.029861213350244498
2116	4	0.03876844542115521
2116	5	0.032039573184036385
2116	6	-0.34883118755997344
2116	7	-0.09238387277015647
2116	8	0.05800288967200373
2116	9	0.08074740729719565
2116	10-14	0.11035174430129757
2116	15-19	0.09779827355188786
2116	20-24	-0.15108844981409675
2116	25-29	-0.22635415853782348
2116	30-34	0.06818692603665966
2116	35-39	-0.1484232964637826
2116	40-44	-0.1753221624347603
2116	45-49	0.008361265933430673
2116	50-54	0.14257058670723666
2116	55-59	0.08993154645499857
2116	60-64	0.277909776659115
2116	65-69	0.2176200224613538
2116	70-74	-0.8274251893706719
2116	75-79	0.15666665199099583
2116	80-84	-0.16150839235491077
2116	85-89	0.07985513116938137
2116	90-94	0.279688353656006
2116	95-99	0.1703360257855664
2116	100-104	0.2680640816272515
2116	105-109	0.29422581724068664
2116	110-114	0.4317058790363717
2116	115-119	0.3391564290835092
2116	120-124	-0.1646698475404591
2116	125-129	0.01662578293290551
2116	130-134	0.15924763180078827
2116	135-139	0.5184143014818687
2116	140-144	0.44925878539409325
2116	145-149	0.6403991596463854
2116	150-154	0.9957788705140587
2116	155-159	-0.40015191037516473
2116	160-164	-0.6478027701585631
2116	165-169	0.6312897184041759
2116	170-174	0.7237598265202649
2116	175-179	0.6639313903534543
2116	180-184	0.6315844000182054
2116	185-189	0.9605416999407765
2116	190-194	1.0299396255945013
2116	195-199	0.9524254816662392
2116	200-204	0.8449105108162449
2116	205-209	0.9353084465137833
2116	210-214	0.7330510631213834
2116	215-219	1.2089539368476174
2116	220-224	1.4026614870993726
2116	225-229	0.9277618293202821
2116	230-234	1.2033005007933681
2116	235-239	1.2809835606991555
2116	240-244	1.7025415663751993
2116	245-249	1.1540064031233932
2116	250-254	0.8659717882823124
2116	255-259	0.7551765149853331
2116	260-264	0.23909527873954417
2116	265-269	0.7683002054468098
2116	270-274	0.5870721743783314
2116	275-279	1.141353905279587
2116	280-284	0.05845063140416329
2116	285-289	-1.1924899000789893
2116	290-294	0.19853601314355807
2116	295-299	0.38686787631585773
2116	300-301	1.3411739435079415
1117	1	-0.08089650657495184
1117	2	0.019541515315530944
1117	3	0.007650405507391156
1117	4	4.6309431359503606E-4
1117	5	0.05213299576422514
1117	6	-0.37777173814455267
1117	7	-0.338383785820497
1117	8	-0.048323664271400446
1117	9	-0.005322290906974558
1117	10-14	0.11204001688527399
1117	15-19	0.0713233899275707
1117	20-24	0.0030335276788164833
1117	25-29	0.11083968607024985
1117	30-34	0.07643973700648132
1117	35-39	-0.1745360508519198
1117	40-44	-0.17402290907471496
1117	45-49	0.036289013985523866
1117	50-54	0.06055225965091893
1117	55-59	0.18470764302708886
1117	60-64	0.16780992946509343
1117	65-69	0.3063944991934662
1117	70-74	-0.1939787141328111
1117	75-79	0.16408023771450786
1117	80-84	-0.007612322962643248
1117	85-89	0.11134781532150839
1117	90-94	0.25879499419483665
1117	95-99	0.3021439829676922
1117	100-104	0.24918183562089524
1117	105-109	0.16953871525677755
1117	110-114	0.29324112021701154
1117	115-119	0.2897073517965225
1117	120-124	0.3921813831997838
1117	125-129	0.3880222427935607
1117	130-134	0.17294719495925648
1117	135-139	0.3422183813531703
1117	140-144	0.3976776702163747
1117	145-149	0.8051027498622929
1117	150-154	0.983183246014228
1117	155-159	-0.052922464711464556
1117	160-164	-0.586966346623548
1117	165-169	0.38934890536043554
1117	170-174	0.8660030311627338
1117	175-179	0.8056087781280112
1117	180-184	0.4869518508956716
1117	185-189	0.8641341701001437
1117	190-194	1.220350527149776
1117	195-199	0.7491350810698307
1117	200-204	0.6855209940470424
1117	205-209	1.4013180528411375
1117	210-214	1.0049429750317138
1117	215-219	0.3733388726578326
1117	220-224	1.0550798953087082
1117	225-229	0.79999542119468
1117	230-234	0.9501599273442523
1117	235-239	0.9865014827954788
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2108	165-169	-0.1869255256540896
2108	170-174	-0.591911653162839
2108	175-179	-0.3846154407224418
2108	180-184	-0.40709244639120357
2108	185-189	-0.7889203496375501
2108	190-194	-0.4800977498207786
2108	195-199	-1.5964123665320713
2108	200-204	-1.037874756242271
2108	205-209	-0.2974305005856266
2108	210-214	0.09456332992434469
2108	215-219	-0.6677123038883899
2108	220-224	-0.37956730155383767
2108	225-229	0.06986603325588447
2108	230-234	-0.41753134178266293
2108	235-239	-0.6581823605888601
2108	240-244	-0.017174763806242055
2108	245-249	-0.0982696638329088
2108	250-254	-0.572026231140125
2108	255-259	-0.6201256828168624
2108	260-264	0.2637898864574062
2108	265-269	-0.9697968428537322
2108	270-274	-0.4628386996313836
2108	275-279	-0.13543148955813322
2108	280-284	-0.5694617759559151
2108	285-289	0.11326495553051785
2108	290-294	-0.6193026017299399
2108	295-299	0.03040256836799138
2108	300-301	0.09189030563418932
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	8.0
13	41.0
14	188.0
15	382.0
16	535.0
17	677.0
18	1450.0
19	1426.0
20	1385.0
21	1717.0
22	2536.0
23	3724.0
24	5298.0
25	7827.0
26	10810.0
27	14310.0
28	19211.0
29	26333.0
30	36466.0
31	50366.0
32	65977.0
33	76656.0
34	65026.0
35	26794.0
36	2378.0
37	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.16871082685291	20.464692569378876	15.386002286928921	29.980594316839294
2	45.48236644951913	23.238186968300887	16.34157798095491	14.937868601225073
3	45.342873274720894	21.83234248895679	18.57062197824096	14.254162258081351
4	45.94829263200847	21.542917874579505	18.117743626727652	14.39104586668438
5	31.5456223340909	21.695458880353762	32.561455283897075	14.19746350165826
6	29.771354554641942	22.439185245987197	32.85301499788862	14.936445201482234
7	17.173317897353897	48.128703804747516	19.921428334195284	14.776549963703307
8	29.16546073077343	20.43669904110304	34.24201591360912	16.15582431451441
9	15.506991265070244	18.982458970502414	35.909054245764196	29.601495518663146
10-14	26.52453229456783	35.1738208319297	22.31667797478685	15.984968898715618
15-19	22.41228299084754	34.69351831203769	21.24789455454705	21.64630414256772
20-24	24.463307126962512	30.84094456806935	26.382239766942018	18.313508538026124
25-29	19.605813164549755	25.823887494484328	36.59660376821358	17.973695572752334
30-34	20.3229219549921	28.11475448726769	30.083458671588463	21.478864886151744
35-39	21.13278896200946	29.5417127294639	27.478447355560508	21.847050952966125
40-44	21.158220370748186	28.56896134520765	28.781712160104	21.49110612394016
45-49	22.019946575062985	29.777522620194247	25.954223464270292	22.248307340472472
50-54	22.427608261412107	28.909248777062384	27.44300470196382	21.22013825956169
55-59	21.92818473830796	26.705683635173155	28.62328776872601	22.742843857792877
60-64	21.995701332776626	26.79436143915203	28.321764256534593	22.88817297153675
65-69	20.197425544331786	27.924066368385343	28.62632435484407	23.252183732438805
70-74	21.46667109502142	26.337212888410207	28.743754833628294	23.45236118294008
75-79	20.77997561241774	25.41987920080849	29.669771259661324	24.130373927112444
80-84	24.699876164222374	25.176477844783	26.839293424367654	23.28435256662697
85-89	21.136347461366558	28.95973202127508	25.70175979654873	24.20216072080963
90-94	20.931140665107254	30.12065685153466	28.11475448726769	20.833447996090396
95-99	21.877132134198128	29.84489687468863	26.41825178043585	21.859719210677394
100-104	22.223350398314697	29.06691402191087	25.584139531132127	23.125596048642315
105-109	24.18712013019363	27.250703396706257	24.07120794446843	24.490968528631683
110-114	20.872544042360378	28.657971275793187	28.708644306638263	21.760840375208172
115-119	23.946945146918576	28.101754102949762	25.58665420401114	22.36464654612052
120-124	19.62080630850766	28.18535511451251	28.02740518971546	24.16643338726437
125-129	21.3890009157205	25.017436646849777	30.573155629783216	23.020406807646506
130-134	24.913433572306335	28.38762021796995	25.299886602487152	21.399059607236566
135-139	23.902819754890565	27.803362070192584	24.682415794043546	23.611402380873304
140-144	21.780720524949825	28.29006988892737	26.53155439996584	23.397655186156964
145-149	20.377722845091405	27.604608019434153	28.83826857655281	23.179400558921632
150-154	23.20824812704317	26.00888201439532	25.928270142292526	24.854599716268986
155-159	22.494840175932207	26.636791087619745	29.16161755146776	21.706751184980284
160-164	25.080113682192795	28.658255955741758	25.982122099229944	20.279508262835506
165-169	20.567035010889008	26.09532982544375	28.324468716045985	25.01316644762126
170-174	22.406304711927618	29.035504334252217	26.181967423124554	22.376223530695615
175-179	21.10455820044315	29.39258788307246	27.300427494389435	22.20242642209496
180-184	20.631182892632957	29.36644477446231	27.079420960984613	22.92295137192012
185-189	22.26904153005983	27.193957193625067	27.92046042237015	22.61654085394495
190-194	20.097512417982482	27.42517777085056	27.668769029084338	24.808540782082616
195-199	20.479021460123455	30.034066700511953	27.821534140242832	21.665377699121763
200-204	22.399851966426745	27.901386865816107	26.72997632411761	22.96878484363954
205-209	21.383781783330093	27.523094660827564	28.412434820153443	22.680688735688904
210-214	23.16170295545233	27.44680043461139	27.25331296290146	22.138183647034822
215-219	22.96237954479676	26.693347504068548	28.615459070140393	21.72881388099429
220-224	18.67248995317015	27.552748822136714	28.634864753301102	25.13989647139204
225-229	21.402855339884137	28.47065186963556	26.44795338840309	23.678539402077213
230-234	21.27380042986672	26.713369993784486	28.632871993661123	23.379957582687663
235-239	21.397304080887064	27.11268106830895	26.151127095363037	25.338887755440947
240-244	22.75883338157077	30.5609143919948	24.160787234951105	22.519464991483325
245-249	20.49472630395278	28.943315477574338	24.9199337644653	25.642024454007583
250-254	21.09340823579091	26.43827427015178	26.234633213609598	26.233684280447704
255-259	21.43369566764565	26.985239344666756	27.41686159335367	24.16420339433392
260-264	21.261464298762117	28.25524404188591	26.68779624507148	23.795495414280495
265-269	20.762040775657965	27.843454496282554	26.85765528105028	24.5368494470092
270-274	19.392777669704834	29.79166172430645	25.649948045909387	25.16561256007933
275-279	20.75274123067142	28.447023433904434	23.889819370572635	26.910415964851513
280-284	17.902288352319903	29.33873592613504	27.067559296460953	25.6914164250841
285-289	20.552373993537763	26.82439517372594	27.741681414669557	24.88154941806674
290-294	19.809738901040504	29.7655660623544	26.211052224536562	24.21364281206853
295-299	19.374795386286966	30.651537508955556	23.403728358393078	26.569938746364404
300-301	15.23108894824993	33.05074894549803	27.002604821529395	24.71555728472265
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.25
29	0.25
30	0.75
31	2.75
32	4.75
33	16.75
34	37.5
35	76.75
36	191.75
37	735.75
38	2368.5
39	4305.0
40	7135.5
41	9453.25
42	14380.0
43	17949.75
44	19587.75
45	15097.0
46	10162.75
47	8202.5
48	7535.0
49	6291.0
50	5342.75
51	2814.75
52	906.75
53	477.25
54	460.0
55	584.0
56	679.5
57	671.75
58	782.5
59	1273.75
60	1469.5
61	900.75
62	334.0
63	78.0
64	17.0
65	6.0
66	4.25
67	4.0
68	1.75
69	1.0
70	1.5
71	0.5
72	0.5
73	0.0
74	0.25
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.25
83	0.25
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	4.74466580946371E-5
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	421526.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.700795867329553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.69385757836704	4.9966143820426465
2	6.39946960646221	1.369588358358638
3	3.5790906466940617	1.1489735520282502
4	3.3956703743950616	1.4534550203656027
5	3.2346969058721515	1.730691564121021
6	2.976384067950448	1.9109807000345802
7	3.19893263482557	2.396178758302497
8	2.563011548144111	2.1941010705838737
9	2.596240089280132	2.5003651696167735
>10	24.3258089480198	46.92867686223818
>50	0.8413660083511765	5.994259701762468
>100	0.13153535352966148	2.458457380959794
>500	0.015432885144879324	1.1173181571499153
>1k	0.030865770067744873	8.470715416592068
>5k	0.015432885033872436	11.632046333799522
>10k+	0.0022046978619817766	3.6975775720442132
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	15673	3.718157361586236	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	9750	2.3130245821135587	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	9002	2.135574080839616	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	7595	1.8017868411438442	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	6840	1.6226757068365891	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5779	1.3709711856445392	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	5209	1.2357482100748234	No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG	5130	1.2170067801274418	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	4656	1.1045582004431518	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	4300	1.0201031490346977	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	4102	0.973130957521007	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3778	0.896267371407695	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	3107	0.7370838335001875	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2528	0.599725758316213	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2515	0.5966417255400616	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1784	0.423224190204163	No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	1749	0.4149210250376015	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT	1588	0.3767264652714186	TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1584	0.37577753210952586	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1467	0.34802123712416316	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	1403	0.3328383065338793	No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC	1344	0.31884154239596135	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	938	0.22252482646384802	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	886	0.21018869535924237	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	704	0.1670122364931226	No Hit
CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG	644	0.15277823906473148	No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT	542	0.12858044343646655	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	518	0.1228868444651101	No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT	504	0.1195655783984855	No Hit
GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG	463	0.1098390134890849	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.372332904731855E-4	0.0	0.0
2	2.372332904731855E-4	0.0	0.0
3	4.74466580946371E-4	0.0	0.0
4	7.116998714195565E-4	0.0	0.0
5	7.116998714195565E-4	0.0	0.0
6	7.116998714195565E-4	0.0	0.0
7	7.116998714195565E-4	0.0	0.0
8	0.0011861664523659277	0.0	0.0
9	0.0011861664523659277	0.0	0.0
10-14	0.002277439588542581	0.0	0.0
15-19	0.0026570128532996777	0.0	0.0
20-24	0.0031314794342460492	0.0	0.0
25-29	0.003321266066624597	0.0	0.0
30-34	0.0058359389456403635	0.0	0.0
35-39	0.006405298842776008	0.0	0.0
40-44	0.007022105398006291	0.0	0.0
45-49	0.014044210796012582	0.0	0.0
50-54	0.024862048841589844	0.0	0.0
55-59	0.02837310154059299	0.0	0.0
60-64	0.03378202056338162	0.0	0.0
65-69	0.04170561246518602	0.0	0.0
70-74	0.056698756423091336	0.0	0.0
75-79	0.07577231297713546	0.0	0.0
80-84	0.10333882133011962	0.0	0.0
85-89	0.13773764844873151	0.0	0.0
90-94	0.18039219407581025	0.0	0.0
95-99	0.23571499741415716	0.0	0.0
100-104	0.30394329175424534	0.0	0.0
105-109	0.38256240421705895	0.0	0.0
110-114	0.47679146719300824	0.0	0.0
115-119	0.5875794138439859	0.0	0.0
120-124	0.7011667133225472	0.0	0.0
125-129	0.8350611824656131	0.0	0.0
130-134	0.9848028354122877	0.0	0.0
135-139	1.1606875969691077	0.0	0.0
140-144	1.3466310500419902	0.0	0.0
145-149	1.5672105635239582	0.0	0.0
150-154	1.8077176734056735	0.0	0.0
155-159	2.086846362976424	0.0	0.0
160-164	2.3913590146278048	0.0	0.0
165-169	2.751953616147047	0.0	0.0
170-174	3.148085764579172	0.0	0.0
175-179	3.6140119470685077	0.0	0.0
180-184	4.1075520845689235	0.0	0.0
185-189	4.716055474632645	0.0	0.0
190-194	5.414043261862851	0.0	0.0
195-199	6.116111461689196	0.0	0.0
200-204	6.853290188505573	0.0	0.0
205-209	7.659598696165835	0.0	0.0
210-214	8.523412553436799	0.0	0.0
215-219	9.401412961478057	0.0	0.0
220-224	10.348258470414637	0.0	0.0
225-229	11.34999976276671	0.0	1.423399742839113E-4
230-234	12.392070714499223	0.0	2.3723329047318549E-4
235-239	13.435280386026012	0.0	2.3723329047318549E-4
240-244	14.542353259348179	0.0	2.3723329047318549E-4
245-249	15.494844920598018	1.423399742839113E-4	2.3723329047318549E-4
250-254	16.566048120400637	2.3723329047318549E-4	2.3723329047318549E-4
255-259	17.628283901823373	2.3723329047318549E-4	2.3723329047318549E-4
260-264	18.664851041216913	2.3723329047318549E-4	2.3723329047318549E-4
265-269	19.61117463691445	2.3723329047318549E-4	2.3723329047318549E-4
270-274	20.515175813591572	2.3723329047318549E-4	2.3723329047318549E-4
275-279	21.36361695363987	2.3723329047318549E-4	2.3723329047318549E-4
280-284	22.124803689452133	2.3723329047318549E-4	2.3723329047318549E-4
285-289	22.848744798660103	2.3723329047318549E-4	2.3723329047318549E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTGGG	10	8.4631285E-4	295.0	295
CGGGTTA	5900	0.0	293.0	1
GGGTTAT	5980	0.0	288.83362	2
GTTATTA	6005	0.0	287.13986	4
GGTTATT	6030	0.0	286.43863	3
GGGGGGA	5720	0.0	285.71677	1
TTATTAG	6210	0.0	277.66104	5
TTAGTAG	6325	0.0	272.61264	8
GGGGAGC	6105	0.0	267.45703	3
GGGAGCG	4725	0.0	265.3439	4
ATTAGTA	6550	0.0	263.47327	7
TATTAGT	6575	0.0	262.47147	6
GGAGCGA	4780	0.0	262.2908	5
CGAAAGC	4790	0.0	261.43527	9
TAGTAGA	6750	0.0	255.44815	9
GCGAAAG	5140	0.0	243.63327	8
GAGCGAA	5140	0.0	243.63327	6
GGGGGAG	6845	0.0	239.18916	2
AGCGAAA	5415	0.0	231.26039	7
GGGAGCA	1735	0.0	218.48703	4
>>END_MODULE