##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJTU1_l01n01_30082_flu.3510000003b987.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	297291
Sequences flagged as poor quality	0
Sequence length	301
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.81377169170947	34.0	34.0	34.0	34.0	34.0
2	33.886121678759196	34.0	34.0	34.0	34.0	34.0
3	33.89028594878419	34.0	34.0	34.0	34.0	34.0
4	33.89592688645132	34.0	34.0	34.0	34.0	34.0
5	33.88723842968674	34.0	34.0	34.0	34.0	34.0
6	37.63519581823869	38.0	38.0	38.0	38.0	38.0
7	37.65581198219926	38.0	38.0	38.0	38.0	38.0
8	37.664937720953546	38.0	38.0	38.0	38.0	38.0
9	37.58444083406494	38.0	38.0	38.0	37.0	38.0
10-14	37.65550252109885	38.0	38.0	38.0	37.6	38.0
15-19	37.62590458506985	38.0	38.0	38.0	37.2	38.0
20-24	37.62116512104302	38.0	38.0	38.0	37.0	38.0
25-29	37.53567716479813	38.0	38.0	38.0	37.0	38.0
30-34	37.485165713055565	38.0	38.0	38.0	37.0	38.0
35-39	37.40661304916732	38.0	38.0	38.0	37.0	38.0
40-44	37.41819765818676	38.0	38.0	38.0	37.0	38.0
45-49	37.39608598982142	38.0	38.0	38.0	37.0	38.0
50-54	37.35512679495847	38.0	38.0	38.0	37.0	38.0
55-59	37.309594303224785	38.0	38.0	38.0	37.0	38.0
60-64	37.13879599449698	38.0	38.0	38.0	36.6	38.0
65-69	37.072407170079146	38.0	38.0	38.0	36.4	38.0
70-74	37.14755239815534	38.0	38.0	38.0	37.0	38.0
75-79	37.08558415828263	38.0	38.0	38.0	36.8	38.0
80-84	36.986740264589244	38.0	38.0	38.0	36.2	38.0
85-89	37.03113918685732	38.0	38.0	38.0	36.0	38.0
90-94	36.873633577874884	38.0	38.0	38.0	35.8	38.0
95-99	36.88199037306881	38.0	38.0	38.0	36.0	38.0
100-104	36.86286433158084	38.0	38.0	38.0	36.0	38.0
105-109	36.72332764866748	38.0	38.0	38.0	35.0	38.0
110-114	36.618157293695404	38.0	37.8	38.0	34.8	38.0
115-119	36.70405965871823	38.0	38.0	38.0	35.0	38.0
120-124	36.61745831525341	38.0	37.8	38.0	34.8	38.0
125-129	36.522257316904984	38.0	37.0	38.0	34.0	38.0
130-134	36.39726463296904	38.0	37.0	38.0	34.0	38.0
135-139	36.157883689718155	38.0	37.0	38.0	33.4	38.0
140-144	36.1038060351642	38.0	37.0	38.0	33.0	38.0
145-149	35.69078243202788	38.0	36.8	38.0	30.4	38.0
150-154	35.32419548523164	38.0	36.2	38.0	28.4	38.0
155-159	35.41679902856124	38.0	36.0	38.0	29.2	38.0
160-164	35.33932544207527	38.0	36.0	38.0	28.6	38.0
165-169	35.14373929920516	38.0	35.6	38.0	28.0	38.0
170-174	35.00413736036408	38.0	35.0	38.0	28.0	38.0
175-179	34.6349341217864	38.0	35.0	38.0	25.0	38.0
180-184	34.34043613832912	37.6	34.8	38.0	23.6	38.0
185-189	33.570459919741936	37.0	33.8	37.8	22.6	38.0
190-194	32.53959521142585	36.8	30.6	37.0	20.6	38.0
195-199	32.30917451251467	36.8	30.0	37.2	20.2	37.8
200-204	32.12201109350771	37.0	30.0	37.0	19.8	37.4
205-209	31.01070197214177	35.4	27.8	37.0	17.4	37.2
210-214	30.27304492904259	34.4	26.0	37.0	15.6	37.0
215-219	29.394473428391713	33.2	24.2	37.0	13.2	37.0
220-224	27.90569576610123	31.4	21.6	36.2	9.2	37.0
225-229	26.8766952245443	29.8	20.0	36.0	9.0	37.0
230-234	26.45390879643178	29.2	19.8	35.8	9.0	37.0
235-239	25.413594087947498	27.6	18.2	34.6	8.8	37.0
240-244	24.319148578328978	25.8	16.2	33.6	8.0	37.0
245-249	23.47032907151579	25.0	15.0	32.8	8.0	36.2
250-254	22.606971620398866	23.2	13.8	31.8	8.0	36.0
255-259	22.003506328815874	22.2	12.8	31.0	8.0	36.0
260-264	20.753985825336116	20.2	11.4	29.2	7.8	35.2
265-269	20.014615982320354	19.6	9.8	28.4	7.0	34.0
270-274	19.027720314439385	18.4	8.8	27.0	7.0	33.4
275-279	17.739136401707416	16.4	8.0	25.0	7.0	31.8
280-284	17.54722006384317	16.4	8.0	24.6	7.0	31.0
285-289	16.42776942457054	14.6	8.0	22.6	7.0	29.6
290-294	15.748967846318925	13.2	8.0	21.4	7.0	28.6
295-299	14.97031931676371	13.0	8.0	20.2	7.0	27.0
300-301	13.145251622147995	10.5	8.0	16.5	7.0	22.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.05163834732883288
1101	2	0.03306968872900029
1101	3	0.07846001673019032
1101	4	0.06126589031623553
1101	5	0.05836488055589939
1101	6	-0.019165409141045586
1101	7	0.12357030243517642
1101	8	0.04424505434047887
1101	9	9.228971317796208E-4
1101	10-14	0.026694728819222746
1101	15-19	0.07457743973236575
1101	20-24	0.05723517000802758
1101	25-29	0.025426451423882668
1101	30-34	0.0847933962405989
1101	35-39	0.1233290894690029
1101	40-44	0.1233749759672591
1101	45-49	0.15623462336178306
1101	50-54	0.11555334908471337
1101	55-59	0.20187768421182994
1101	60-64	0.2648270598675708
1101	65-69	0.3792238160678707
1101	70-74	0.17877491625568354
1101	75-79	0.09908875603753131
1101	80-84	0.3100828418298889
1101	85-89	0.19627144323381174
1101	90-94	0.3899776718057524
1101	95-99	0.26798938541045203
1101	100-104	0.2889316946876974
1101	105-109	0.2519050583609186
1101	110-114	0.217084337687659
1101	115-119	0.185267699827385
1101	120-124	0.13539475330873074
1101	125-129	0.2172505527469326
1101	130-134	0.2720929842287845
1101	135-139	0.41634946419916474
1101	140-144	0.3034854101467559
1101	145-149	0.614233470131488
1101	150-154	0.8144908622066183
1101	155-159	0.5361660045295764
1101	160-164	0.4298522372315432
1101	165-169	0.2572283844270302
1101	170-174	0.3887229491184385
1101	175-179	0.3488652261887921
1101	180-184	0.3020174766954611
1101	185-189	0.3550325794785536
1101	190-194	0.5403915292380361
1101	195-199	0.2554626653286718
1101	200-204	0.055515566471321165
1101	205-209	-0.028697743216362426
1101	210-214	-0.3187044263236132
1101	215-219	-0.06907824403365481
1101	220-224	0.31589184494854905
1101	225-229	0.5135254577528592
1101	230-234	0.21042627532469282
1101	235-239	0.4750423478710424
1101	240-244	0.30932020183973563
1101	245-249	0.32046396690573076
1101	250-254	0.8769596306595986
1101	255-259	0.2548223926002855
1101	260-264	0.4383012915095357
1101	265-269	0.26879578269566906
1101	270-274	0.17062125069911005
1101	275-279	0.3306607817567375
1101	280-284	-0.14148682709768323
1101	285-289	0.4200592392478839
1101	290-294	0.2697480584347858
1101	295-299	0.24868271370007378
1101	300-301	0.16937285991437356
1102	1	-0.03409526949163677
1102	2	-0.03815728690974396
1102	3	0.042913373830600676
1102	4	0.011206472609757157
1102	5	-0.02603797149401288
1102	6	-0.018734630769088767
1102	7	0.05654712952964758
1102	8	-0.09676801755495035
1102	9	-0.004008772505777358
1102	10-14	0.020262762093139486
1102	15-19	0.044809168787622866
1102	20-24	0.01505751106566322
1102	25-29	0.0077585964030788546
1102	30-34	0.014897377226930075
1102	35-39	-0.015126050162592719
1102	40-44	-0.004331500562749113
1102	45-49	0.03585137915500525
1102	50-54	0.02705067528791716
1102	55-59	0.026815295620032487
1102	60-64	0.16215920484678037
1102	65-69	0.20677877744635964
1102	70-74	0.14866202261338657
1102	75-79	0.008016266435625141
1102	80-84	0.12261997789644141
1102	85-89	0.07326193640078316
1102	90-94	0.2029218191616664
1102	95-99	0.06309633728211139
1102	100-104	0.02996259189505679
1102	105-109	0.036596086287246976
1102	110-114	0.14364999425331604
1102	115-119	0.08309301177035167
1102	120-124	0.2236954069034951
1102	125-129	0.05287740955025555
1102	130-134	0.29385471922580564
1102	135-139	0.10097216176303903
1102	140-144	0.29191084092512654
1102	145-149	0.468137213447001
1102	150-154	0.9707000125334204
1102	155-159	0.6001856123727123
1102	160-164	0.5322764796557848
1102	165-169	0.6171214325553791
1102	170-174	0.5561085700334658
1102	175-179	0.5705942814472493
1102	180-184	0.7529741017697376
1102	185-189	0.8197265782901937
1102	190-194	0.8425008578179458
1102	195-199	0.8846664680618872
1102	200-204	0.6859968498937832
1102	205-209	0.23764212606468504
1102	210-214	-0.02248042157019725
1102	215-219	0.22815586172986357
1102	220-224	1.0364741384720233
1102	225-229	1.3617845189530975
1102	230-234	0.8904648968338975
1102	235-239	1.3306959426422758
1102	240-244	1.3228674389163828
1102	245-249	1.6684378231148784
1102	250-254	0.8405603436720774
1102	255-259	1.14371722325982
1102	260-264	1.500743359245721
1102	265-269	1.4625866323688683
1102	270-274	1.1905440076806855
1102	275-279	1.4548497300633372
1102	280-284	1.0907027153859765
1102	285-289	1.3386302434071204
1102	290-294	1.4915959491062072
1102	295-299	1.0711218105509346
1102	300-301	0.5476036977040337
1103	1	-0.001816509316810766
1103	2	0.04794079240378579
1103	3	0.029400402179774687
1103	4	0.04053403185431392
1103	5	0.02810213035449749
1103	6	-0.021509401208966494
1103	7	0.06018801328601597
1103	8	-0.018689505986721144
1103	9	0.01218273477198295
1103	10-14	0.06280336115484886
1103	15-19	0.006273541857915177
1103	20-24	0.040771453084175846
1103	25-29	-0.04927175766083991
1103	30-34	-0.04905707162828321
1103	35-39	-0.5115485209914254
1103	40-44	-0.4032328197816355
1103	45-49	-0.2633089721020596
1103	50-54	-0.039174915152756284
1103	55-59	-0.3942585245010619
1103	60-64	0.0857968807760443
1103	65-69	0.23278302781725557
1103	70-74	0.060376205218943824
1103	75-79	0.1555314033697428
1103	80-84	0.17412324587029815
1103	85-89	0.13955489121051556
1103	90-94	0.25708277188631
1103	95-99	0.1598363829936531
1103	100-104	0.22568976620036807
1103	105-109	-0.05305466153744476
1103	110-114	0.2004461769390673
1103	115-119	0.01619538155261324
1103	120-124	0.2837587614886914
1103	125-129	0.2074321230511984
1103	130-134	0.28098652082170616
1103	135-139	0.24459139268649466
1103	140-144	0.2941082024873296
1103	145-149	0.6596396794640782
1103	150-154	0.6694738206251571
1103	155-159	-0.29607325927410244
1103	160-164	-0.8834835748772747
1103	165-169	0.18753885105652301
1103	170-174	0.6252260166031007
1103	175-179	0.48930954669445725
1103	180-184	0.40885765764606674
1103	185-189	0.3261350148754616
1103	190-194	0.23563290000087989
1103	195-199	0.5016775751632068
1103	200-204	0.3664395300333254
1103	205-209	0.8611250243364665
1103	210-214	0.3896227100710057
1103	215-219	0.11867753461169528
1103	220-224	0.8920497429469378
1103	225-229	1.4987779830053825
1103	230-234	0.7546535170672719
1103	235-239	1.0087199211498437
1103	240-244	0.5522302842590214
1103	245-249	0.7447039123726675
1103	250-254	1.1581977790143085
1103	255-259	0.46884172704906746
1103	260-264	1.687296148050411
1103	265-269	0.4775142557699681
1103	270-274	0.7089288667367839
1103	275-279	1.7016535313384438
1103	280-284	0.6286392806051104
1103	285-289	0.7173799927956317
1103	290-294	0.970906266280112
1103	295-299	0.8561893754091905
1103	300-301	-0.6260359831171698
2116	1	0.13286233146901338
2116	2	0.053724759784813614
2116	3	0.02894046859781696
2116	4	0.01800031221711862
2116	5	0.0424629512754251
2116	6	-0.3798203556467925
2116	7	-0.14102385118486893
2116	8	0.059709501525020414
2116	9	0.08425311671354052
2116	10-14	0.057196105097418126
2116	15-19	0.07666365328589819
2116	20-24	-0.1899926569670285
2116	25-29	-0.17318512674996356
2116	30-34	0.08917475607817948
2116	35-39	-0.0704670718980509
2116	40-44	-0.04267454513774993
2116	45-49	0.11116773997800067
2116	50-54	0.27867096170910344
2116	55-59	0.23218009176454757
2116	60-64	0.3820155912977796
2116	65-69	0.3084236441887711
2116	70-74	-0.6599750993130726
2116	75-79	0.2329125052435188
2116	80-84	-0.008811402711700111
2116	85-89	0.033077543695895884
2116	90-94	0.3525961500530812
2116	95-99	0.33453368164032327
2116	100-104	0.21002206821333402
2116	105-109	0.27089925432799333
2116	110-114	0.3804659964453947
2116	115-119	0.36313266264919264
2116	120-124	-0.05155551385116297
2116	125-129	0.04246695848122073
2116	130-134	0.17781948474566178
2116	135-139	0.45897672322593763
2116	140-144	0.44289429554823556
2116	145-149	0.702332711621608
2116	150-154	1.0469473630971535
2116	155-159	-0.33211389682676895
2116	160-164	-0.541519930811404
2116	165-169	0.6063334922251116
2116	170-174	0.9733715311159727
2116	175-179	0.6116851641628571
2116	180-184	0.7108107111360482
2116	185-189	1.041824045308239
2116	190-194	0.8066764499619197
2116	195-199	0.7090646531476779
2116	200-204	0.8379129060819821
2116	205-209	0.9961618265877235
2116	210-214	0.6982926093848896
2116	215-219	1.0320635686679616
2116	220-224	1.2887436677387214
2116	225-229	1.2467927795528553
2116	230-234	1.144482933148307
2116	235-239	0.9209972732032767
2116	240-244	1.6404016700358746
2116	245-249	0.7901447450945192
2116	250-254	1.157073967617336
2116	255-259	1.1101319617816188
2116	260-264	0.18778316324788236
2116	265-269	0.6116578185909951
2116	270-274	1.090081575276571
2116	275-279	1.9473349214271778
2116	280-284	0.2110785928228509
2116	285-289	-1.0761927379843659
2116	290-294	0.37085966774614754
2116	295-299	0.71526935695133
2116	300-301	1.5557232628338262
1117	1	0.0012182859181351091
1117	2	0.027347973434793005
1117	3	0.04830249354292704
1117	4	0.018787200068032917
1117	5	0.03218298727363589
1117	6	-0.3674578127308479
1117	7	-0.2983815581783915
1117	8	-0.07672768419731568
1117	9	0.08640598075612616
1117	10-14	0.14149608813731618
1117	15-19	0.14279493062642956
1117	20-24	0.06146614143230522
1117	25-29	0.16883544845742193
1117	30-34	0.1410445696850786
1117	35-39	-0.10332781413956127
1117	40-44	-0.03145398276504352
1117	45-49	0.07944347770446569
1117	50-54	0.13101840860181824
1117	55-59	0.21575812430045715
1117	60-64	0.2241695554554255
1117	65-69	0.2983209209456419
1117	70-74	-0.022907333753693138
1117	75-79	0.1886319575695765
1117	80-84	-0.005951103572087391
1117	85-89	0.18006758307085136
1117	90-94	0.31944051585967514
1117	95-99	0.35582029675437354
1117	100-104	0.19692519849903078
1117	105-109	0.179972911614648
1117	110-114	0.2834599901227932
1117	115-119	0.2225550716872462
1117	120-124	0.3818011969270714
1117	125-129	0.3653336138411447
1117	130-134	0.24636826491670405
1117	135-139	0.35206723671991114
1117	140-144	0.4451999533482791
1117	145-149	0.766018983255293
1117	150-154	1.0041232455193025
1117	155-159	-0.01758244652370422
1117	160-164	-0.4739853956023694
1117	165-169	0.4017704594523863
1117	170-174	0.5723041139263643
1117	175-179	0.6710855708998409
1117	180-184	0.7513209200509507
1117	185-189	1.11181978236241
1117	190-194	1.012377185052685
1117	195-199	0.7735262003067049
1117	200-204	0.6890291408770963
1117	205-209	1.4651271225415883
1117	210-214	0.9017200789521951
1117	215-219	0.4769329834831524
1117	220-224	1.1823069251182794
1117	225-229	0.7422476236497495
1117	230-234	1.339415986563477
1117	235-239	1.0502488740998714
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2108	190-194	-0.9756491593378698
2108	195-199	-1.7625697717178497
2108	200-204	-1.088359806981515
2108	205-209	-0.7097121122589058
2108	210-214	-0.18756557300165966
2108	215-219	-1.0903792825882128
2108	220-224	-0.3425090655650358
2108	225-229	-0.25789295169371584
2108	230-234	-0.25854868555168053
2108	235-239	-0.8336822217690241
2108	240-244	-0.6179240718834116
2108	245-249	-0.24678599752733277
2108	250-254	-0.4837317905952041
2108	255-259	-0.706402532709145
2108	260-264	-0.28670226518416086
2108	265-269	-1.0766231958219024
2108	270-274	-1.0037776680660073
2108	275-279	-0.07375663181560554
2108	280-284	-0.7892283265997477
2108	285-289	-0.23344410404419236
2108	290-294	-0.7147803239809196
2108	295-299	-0.028358828482311083
2108	300-301	-0.22628263873408372
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	17.0
15	93.0
16	187.0
17	293.0
18	675.0
19	700.0
20	737.0
21	1035.0
22	1560.0
23	2297.0
24	3546.0
25	5045.0
26	7197.0
27	9950.0
28	13512.0
29	18425.0
30	25462.0
31	34918.0
32	46361.0
33	55234.0
34	48394.0
35	19950.0
36	1697.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.93225829238019	23.971462304610633	15.633840244070624	31.462439158938547
2	41.63361823936816	24.717532653191654	18.416971923132554	15.231877184307633
3	40.89932086743292	23.565126424950638	20.06317042897363	15.47238227864281
4	41.172117554853656	23.27382934565796	20.10454403261451	15.44950906687387
5	27.41455341735875	23.197473182841055	34.109004308909455	15.278969090890742
6	26.128271626117172	23.19646407055713	34.607842147929134	16.067422155396564
7	17.915503380537523	45.40549631672777	21.12213663426284	15.556863668471863
8	25.810064885919857	21.98922940822292	35.398313437002805	16.802392268854422
9	16.259826230864707	20.35682210359547	37.25238907333219	26.130962592207634
10-14	25.607704235916994	33.69520099834842	24.106077883286073	16.59101688244851
15-19	21.38107107177816	34.756518024427244	22.557763268985607	21.304647634808994
20-24	24.612584975663577	30.743345745414423	26.67541230646067	17.968656972461325
25-29	19.458644896750997	26.099343740644688	36.44086097460064	18.001150388003673
30-34	19.6859642572429	28.56110679435301	30.72484535354249	21.0280835948616
35-39	20.96121308751358	29.818662522578887	27.9959366412034	21.224187748704132
40-44	20.557299077335	29.263381669811732	28.47324675149937	21.706072501353894
45-49	20.849268898150296	30.486358483775156	26.70232196736531	21.962050650709237
50-54	21.87540154259631	29.40331190651584	28.049957785469452	20.671328765418394
55-59	21.21174203053574	27.554618202367376	29.753742965646452	21.47989680145043
60-64	22.067200150694102	26.51085973002883	28.26012223713466	23.16181788214241
65-69	20.525747499924314	27.707397802153444	28.893710203134304	22.873144494787937
70-74	21.171848458244614	26.85812890400315	29.137175360168992	22.832847277583245
75-79	20.486055750089978	26.396090026270556	30.423053506497	22.694800717142463
80-84	22.935911278847996	26.235102979908575	27.706254141565	23.122731599678428
85-89	21.087621219613105	28.702180691645555	26.416204997796772	23.79399309094456
90-94	20.499846951303606	29.972114863887573	28.765552943075978	20.762485241732847
95-99	22.038810458439713	30.256549979649566	26.712884009270375	20.991755552640342
100-104	22.520762485241733	28.898217571335827	26.59320329239701	21.987816651025426
105-109	23.330608730166738	28.071620062497686	24.560178411051798	24.03759279628378
110-114	21.702372422979504	28.23489443003656	28.98977769256385	21.072955454420082
115-119	23.14304839366143	28.082182104402758	27.105361413564488	21.669408088371327
120-124	18.847661045911245	29.365369284640302	27.80211980853776	23.98484986091069
125-129	20.951794706196956	26.00495810502168	30.139896599627974	22.903350589153387
130-134	24.351224894127302	29.14423914615646	26.049157223057545	20.455378736658695
135-139	23.72537345563774	27.822772973282074	25.3978761550131	23.053977416067088
140-144	21.229367858428276	29.587777632017115	26.6476953557289	22.535159153825713
145-149	20.81805369150092	27.56168198835485	28.683142106555533	22.937122213588708
150-154	22.742228994486883	26.517519871102724	25.787393496607702	24.95285763780269
155-159	21.18126683956124	27.830509500792154	29.665142907117943	21.323080752528668
160-164	24.358894147485124	29.003165248863912	26.19541123007424	20.442529373576733
165-169	19.994618067819072	26.533598393493246	28.219825019929967	25.251958518757718
170-174	21.886636326023996	29.262574379984596	26.388891691978568	22.461897602012844
175-179	20.224964765162753	29.50873050310975	28.405568954324213	21.860735777403285
180-184	19.780215344561388	29.840526622063905	26.66168837939931	23.7175696539754
185-189	21.73130030845199	28.177375029852904	27.658153122697964	22.433171538997144
190-194	20.132395531650808	28.653137834646863	26.939396079935147	24.275070553767183
195-199	20.660363078599755	30.282585076574804	27.382934565795804	21.674117279029637
200-204	21.346761254124747	28.667669051535366	27.559932860396042	22.425636833943845
205-209	21.04799674393103	28.17798049722326	27.84833715114147	22.925685607704235
210-214	22.331654843234404	28.124564820327556	27.593502662374576	21.95027767406346
215-219	22.074196662529307	28.368097251514506	27.230693159227826	22.327012926728358
220-224	19.275793750903997	28.226552435156126	27.83299864442583	24.664655169514045
225-229	20.831777618562285	29.547614963116946	26.545842289204852	23.074765129115917
230-234	20.34511640110195	28.361571658745135	27.821158393627794	23.47215354652512
235-239	20.85734179642169	28.40065794120912	26.18088001318573	24.56112024918346
240-244	21.93978290631065	31.982737452529676	23.69610919940395	22.38137044175572
245-249	19.75734213279245	30.47431641052033	24.978018170748527	24.790323285938694
250-254	20.18076564712689	28.366684494317017	26.076941447941582	25.375608410614515
255-259	20.261023710774964	29.500993975599666	26.81588073638287	23.4221015772425
260-264	20.303742797461073	30.113996051007263	26.47870268524779	23.10355846628388
265-269	19.513136960082882	29.633927700468565	26.601343464820665	24.25159187462789
270-274	18.469513036048856	31.839510782364755	25.00560057317578	24.685375608410613
275-279	19.745367333689888	30.380468968115416	24.416615370125566	25.45754832806913
280-284	17.21781015906973	31.444947879350536	26.653750029432445	24.683491932147287
285-289	19.33701322946204	29.39153892987006	27.640392746500904	23.631055094166996
290-294	18.832726184109173	31.912435963416314	25.447793575991202	23.80704427648331
295-299	18.340413937858866	32.70775099145282	23.277933068945917	25.6739020017424
300-301	14.288525384219502	35.9423931434184	27.650685691796927	22.11839578056517
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.25
29	0.75
30	1.0
31	1.5
32	3.5
33	8.25
34	25.25
35	77.75
36	219.75
37	647.75
38	2012.0
39	3410.0
40	5842.0
41	7737.5
42	10402.75
43	14502.0
44	14685.75
45	11347.0
46	8468.75
47	6333.0
48	5230.75
49	3464.5
50	2293.0
51	1850.5
52	484.0
53	79.75
54	27.5
55	12.0
56	8.25
57	7.75
58	4.25
59	5.0
60	3.25
61	1.5
62	1.75
63	0.75
64	1.0
65	1.0
66	0.25
67	0.5
68	0.25
69	0.0
70	0.0
71	0.25
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.25
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	3.363707613079441E-4
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	297291.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.923387206485494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	32.95185088924304	3.5994582643357504
2	4.823782912121099	1.053840970982539
3	4.972119517190735	1.629371601695943
4	5.016313820102565	2.191805528248989
5	5.762774008188761	3.1474505937458117
6	4.724882304991692	3.0967031353497565
7	5.007594171867023	3.8289923078570496
8	4.784323681353039	4.18088160740619
9	4.46359782810442	4.388184666937104
>10	27.07941204989503	48.36318567434531
>50	0.29877353448389954	2.059178768571832
>100	0.04343710968256342	0.8307020348765441
>500	0.01855778420256125	1.4392684240239746
>1k	0.04639446050609536	14.153819738814988
>5k	0.006185928067479381	6.037156682808226
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	9583	3.2234410056140277	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	8286	2.787168128197625	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	4822	1.6219798110269061	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	4676	1.5728696798759465	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG	4412	1.4840677988906492	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4297	1.4453851613402358	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	3650	1.227753278773996	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	3482	1.1712429908742612	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3006	1.01113050849168	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2268	0.7628888866464172	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	2256	0.7588524375107218	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2141	0.7201697999603083	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1851	0.6226222791810044	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1397	0.46990995354719783	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1275	0.4288727206676287	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1249	0.4201270808736221	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1111	0.37370791581312585	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA	818	0.27515128274989825	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	805	0.270778462852895	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	744	0.2502598464131104	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT	694	0.2334413083477132	TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	694	0.2334413083477132	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	505	0.16986723446051175	No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG	462	0.15540329172427017	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	3.3637076130794405E-4	0.0	0.0
2	3.3637076130794405E-4	0.0	0.0
3	3.3637076130794405E-4	0.0	0.0
4	3.3637076130794405E-4	0.0	0.0
5	3.3637076130794405E-4	0.0	0.0
6	6.727415226158881E-4	0.0	0.0
7	6.727415226158881E-4	0.0	0.0
8	6.727415226158881E-4	0.0	0.0
9	6.727415226158881E-4	0.0	0.0
10-14	0.0011436605884470098	0.0	0.0
15-19	0.001412757197493365	0.0	0.0
20-24	0.0016818538065397203	0.0	0.0
25-29	0.0016818538065397203	0.0	0.0
30-34	0.004036449135695329	0.0	0.0
35-39	0.005449206333188694	0.0	0.0
40-44	0.007534705053297947	0.0	0.0
45-49	0.010696590209592623	0.0	0.0
50-54	0.01567487747695019	0.0	0.0
55-59	0.022873211768940197	0.0	0.0
60-64	0.03356980197853282	0.0	0.0
65-69	0.04998469513036048	0.0	0.0
70-74	0.07238698783346956	0.0	0.0
75-79	0.10198761482856863	0.0	0.0
80-84	0.14315939601266098	0.0	0.0
85-89	0.2032352139822598	0.0	0.0
90-94	0.28806791998412334	0.0	0.0
95-99	0.38292447467296353	0.0	0.0
100-104	0.507516204661426	0.0	0.0
105-109	0.6599594336861863	0.0	0.0
110-114	0.8416669189447378	0.0	0.0
115-119	1.072148164592941	0.0	0.0
120-124	1.3371410503513392	0.0	0.0
125-129	1.633618239368161	0.0	0.0
130-134	1.9966295649716943	0.0	0.0
135-139	2.398996269648257	0.0	0.0
140-144	2.824370734398283	0.0	0.0
145-149	3.2973080247972524	0.0	0.0
150-154	3.8458614623382474	0.0	0.0
155-159	4.512211940489285	0.0	0.0
160-164	5.180984288121739	0.0	0.0
165-169	5.894090302094581	0.0	0.0
170-174	6.672115872999855	0.0	0.0
175-179	7.515128274989825	0.0	0.0
180-184	8.411892724636804	0.0	0.0
185-189	9.409702950980689	0.0	0.0
190-194	10.497391444746057	0.0	0.0
195-199	11.544917269611256	0.0	0.0
200-204	12.63536400361935	0.0	0.0
205-209	13.803512383489576	0.0	0.0
210-214	15.013370737761992	0.0	0.0
215-219	16.235136616984704	0.0	0.0
220-224	17.49753608417342	0.0	0.0
225-229	18.823778721858382	0.0	0.0
230-234	20.152241406567978	0.0	0.0
235-239	21.450497996912116	0.0	0.0
240-244	22.737990722894402	0.0	0.0
245-249	23.978660638902625	0.0	0.0
250-254	25.320779976521322	0.0	0.0
255-259	26.615672859252385	0.0	0.0
260-264	27.861321062527963	3.3637076130794405E-4	0.0
265-269	29.000743379382488	3.3637076130794405E-4	2.0182245678476642E-4
270-274	30.08876824390917	3.3637076130794405E-4	3.3637076130794405E-4
275-279	31.09619867402646	3.3637076130794405E-4	3.3637076130794405E-4
280-284	32.057949954758136	3.3637076130794405E-4	3.3637076130794405E-4
285-289	32.95343619551214	3.3637076130794405E-4	3.3637076130794405E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	10	8.461875E-4	295.0	295
GGTTAGG	10	8.461875E-4	295.0	295
CGGGTTA	4000	0.0	292.7875	1
GGGTTAT	4025	0.0	291.70187	2
GGGGGGA	2775	0.0	287.55856	1
GTTATTA	4085	0.0	287.4174	4
GGTTATT	4125	0.0	284.6303	3
TTAGTAG	4240	0.0	276.5625	8
TTATTAG	4255	0.0	275.93417	5
TAGTAGA	4265	0.0	274.9414	9
GGGGAGC	2950	0.0	271.0	3
TATTAGT	4385	0.0	267.41733	6
GGGAGCG	1900	0.0	263.94736	4
GGAGCGA	1910	0.0	262.56543	5
GCGAAAG	1975	0.0	252.43037	8
ATTAGTA	4655	0.0	251.90656	7
CGAAAGC	2010	0.0	247.30101	9
GAGCGAA	2140	0.0	233.65654	6
AGCGAAA	2215	0.0	225.74492	7
GGGGGAG	3565	0.0	224.24963	2
>>END_MODULE