FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_plasmids_flu.3520000003ec2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_plasmids_flu.3520000003ec2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536546
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGG6025111.229419285578498No Hit
GGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAACAAAG103411.9273277594092586No Hit
GGCCGCGCTCCCCTCGCGCACACTGCGGAGCGCACAAAGCCCCGCGCCGC36380.6780406526187875No Hit
GGCCGCCCGTGCCACACGCAGGGGCCGGCCCGTCTAGAATTGACCTGCAT24510.45681078602766584No Hit
CGCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGG12620.2352081648171825No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT7660.1427650192155006Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGT67150.0268.860788
GGGCGTC72100.0253.061729
GAGGGCG76100.0243.636667
GGAGGGC90200.0206.042146
GGCGGTG18100.0184.986198
GCCGGGA102800.0181.218382
CGGGAGG106050.0174.830284
CCGGGAG109850.0169.453813
GGGAGGG116400.0159.284795
GGCCGGG138150.0156.20161
CGTGCCA5100.0147.58
GGGCGGT25700.0130.856037
CGGGGGC29900.0114.941474
CGCGCTC10750.0109.767454
CCGCGCT11100.0106.3063053
CCCGTGC8050.0100.77646
GGGGGCG38500.089.2662355
CGGCCAT2700.087.4074295
CTCCCCT13550.085.9963158
GCGGTGC39650.084.817159
Produced by FastQC (version 0.11.2)